Carg14280 (gene) Silver-seed gourd

NameCarg14280
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionmitotic spindle checkpoint protein MAD2
LocationCucurbita_argyrosperma_scaffold_040 : 110874 .. 113138 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTTCTCAGATCCACTCGTTTTTCTCTTTGTCAATTTGATCACTCGATCGATTTCAGTTTCCAATTTGGTTACCACTGTATCGATTACAAGCCCTAAATCCGCTCCTCGTCTTAGAATCGAAGAAATTCAGGAACAATTCTTATTGTTTCAGAAGAGATTTGTAGAAGATTTGGTGGAATGGCTTCGAAAACAGCGTCCAAAGACATCATCACTCTTCGCGGTTCTGCGGCGATTGTCAGCGAGTTCTTCGGTATGTTATGCTTTAGTGCCTTCTGAAACCCTATTTATCGCAATTTTCTTTTTACTTCTGGCGATTATCGATGAATATCCTTGGCTGATTAGTTCTTTTTCGTTGTCCTGAAATTCATCATTTAGGGTATGCTGCTAATAGGTATGAATACTGGATGCGCTTTCTCTGTTCTTATTTTTGTTTTATGATCATGTGAATTTGCATTCTCTTACTTACAAACGATGTCGATTGTCAATGTGCAGCATCTTGTACAATCGTGGACTTTATCCTGAAGAAAGTTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTCACTGCTCAGCTTTCTGGTAAGTTCCCCTTCTCTAAATGAAAGATTTGTATGCTTTTATTTACTTTCCTGAGAAGTGTATTTATTGAGAAGTTTTTGAATCGTGAAATTGGTTTTAGAATGGCTGGAAGCTGGGAAGCTACAAAGGGTTGTGCTGGTTATAATGAGCAAAGCCAACAATGAGGTGCTTGAGAGGTGGAATTTCAGAATTGAAACTGATGGAGAGGTGGTCGAGCAAGGGTAAGAAATTCATGGAAAACCTTCTCTTCCCTTGCATAGACTTCTGGATTTGGTGTTTGATTAGATAAACATTGTTGCAGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTAAGTGTTGCCAAAATTATCATAATCTATCATTTATTGATTTTGGGATTGGTGTAACATAATGTGATATTGTTAAGTCGGATTCTGAATCCATGGGATGTCCTGAAGTCATTATGCAGAACCTTGCAAGCGATGCTTCAAGTATGTGTCACTCCCATGTTCAGCCTAGATGATGAACCCTTTATGTGTTGGCAAAATTACCACGATGTGCCATATAATTTAACATATCATCATGAAATCAATACTAGCCAACTAGAACATAGCTTGAATGGTTGGGCATGTGTCACTTTCTTATGGGGTCTATGTTCTAATCATTCCCCTTGTTTGCATTCTAAAAAGGAATCATTAGCTTATTATAATGATTCGTGAGTGTTTTAAAAAGATAATCGAGATGTTTGAGCTCTTTTGCTTTTTGGACGATATTGATCCATGATGCTCAATCAAGGCACTCTTGATGTTTTTGTTGTGTCAGGTGTATTTGATGTGTTAGCATACACAGATAAAGATCTTGCAGTTCCATTTACTTGGATCGAGAGTGACCCTAAGCTGATTGCTAATCCACAGATGGTGAAGCTGCATTCATTTGATACAAAGGTGAGGGAATGGGGGGACTCCGTGGAGGAATATCAAAACTTATTTGGTAAGTTAATTAACTTTTAAATGGTGTTATTGCAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGACGACGAGTAAAAAACAAACTCGCTTTCCTGGAATTTGTTGTTGGGTGTTCTGGGATCCCTCCAACCCGATCGCTTTCAAGGTGGCATTTTGTGAATCTTTTATTCACATTTGGTGTTGTACTGTAATGTAATGTGTAATGTAGGAACAATGTTTTGTGGGAGTGAGTGGTGAATATAGGGTAGAGGATGGGTTCACTTAGAATTCCACCTGTGAATGGTTTTGGGTTTGTTTGTTTGTTGTGTGTAGTTGGGGGAACTTTTGAAGACCATATGACCTGACCATGTTTGTACGGACAAAGGTTTTATCTTAAGAACAAGGGTGGACCAAGACATAGTTCAGACAGACATATTCACAGTAAGAGGTTCAAATCACAATTCCAAATAAACTGAAGGAATTACAATTAAACGTCTAAGAAATTGTAACTGATGGAAATTGAAACACCAAACAGGACAGATAAAATCGGAACTTTATAAGAATCTTCATTTATTTCATTATTGAAACTTCCCAACA

mRNA sequence

AAATTTTCTCAGATCCACTCGTTTTTCTCTTTGTCAATTTGATCACTCGATCGATTTCAGTTTCCAATTTGGTTACCACTGTATCGATTACAAGCCCTAAATCCGCTCCTCGTCTTAGAATCGAAGAAATTCAGGAACAATTCTTATTGTTTCAGAAGAGATTTGTAGAAGATTTGGTGGAATGGCTTCGAAAACAGCGTCCAAAGACATCATCACTCTTCGCGGTTCTGCGGCGATTGTCAGCGAGTTCTTCGTTCTTTTTCGTTGTCCTGAAATTCATCATTTAGGGTATGCTGCTAATAGCATCTTGTACAATCGTGGACTTTATCCTGAAGAAAGTTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTCACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGCTACAAAGGGTTGTGCTGGTTATAATGAGCAAAGCCAACAATGAGGTGCTTGAGAGGTGGAATTTCAGAATTGAAACTGATGGAGAGGTGGTCGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATACACAGATAAAGATCTTGCAGTTCCATTTACTTGGATCGAGAGTGACCCTAAGCTGATTGCTAATCCACAGATGGTGAAGCTGCATTCATTTGATACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGACGACGAGTAAAAAACAAACTCGCTTTCCTGGAATTTGTTGTTGGGTGTTCTGGGATCCCTCCAACCCGATCGCTTTCAAGGTGGCATTTTGTGAATCTTTTATTCACATTTGGTGTTGTACTGTAATGTAATGTGTAATGTAGGAACAATGTTTTGTGGGAGTGAGTGGTGAATATAGGGTAGAGGATGGGTTCACTTAGAATTCCACCTGTGAATGGTTTTGGGTTTGTTTGTTTGTTGTGTGTAGTTGGGGGAACTTTTGAAGACCATATGACCTGACCATGTTTGTACGGACAAAGGTTTTATCTTAAGAACAAGGGTGGACCAAGACATAGTTCAGACAGACATATTCACAGTAAGAGGTTCAAATCACAATTCCAAATAAACTGAAGGAATTACAATTAAACGTCTAAGAAATTGTAACTGATGGAAATTGAAACACCAAACAGGACAGATAAAATCGGAACTTTATAAGAATCTTCATTTATTTCATTATTGAAACTTCCCAACA

Coding sequence (CDS)

ATGGCTTCGAAAACAGCGTCCAAAGACATCATCACTCTTCGCGGTTCTGCGGCGATTGTCAGCGAGTTCTTCGTTCTTTTTCGTTGTCCTGAAATTCATCATTTAGGGTATGCTGCTAATAGCATCTTGTACAATCGTGGACTTTATCCTGAAGAAAGTTTTGCAAGGGTGAAGAAATATGGACTTCCAATGTTGCTTACTCAAGATGAAGGTGTTAAGACCTTCATTTCTAATCTCACTGCTCAGCTTTCTGAATGGCTGGAAGCTGGGAAGCTACAAAGGGTTGTGCTGGTTATAATGAGCAAAGCCAACAATGAGGTGCTTGAGAGGTGGAATTTCAGAATTGAAACTGATGGAGAGGTGGTCGAGCAAGGTGTGTCGAGGGAAAAGAGTGATAAAGAAATAATGAGGGAGATCCAAGCAATTATGAGACAGATTGCTTCAAGCATCACTTACTTGCCCTGCCTAGATGAACCCTGTGTATTTGATGTGTTAGCATACACAGATAAAGATCTTGCAGTTCCATTTACTTGGATCGAGAGTGACCCTAAGCTGATTGCTAATCCACAGATGGTGAAGCTGCATTCATTTGATACAAAGATTCACAAAGTGGACACTCTTGTTTCTTACAAGAACGATGACTGCGACGACGAGTAA

Protein sequence

MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKYGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGEVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE
BLAST of Carg14280 vs. NCBI nr
Match: XP_022956917.1 (mitotic spindle checkpoint protein MAD2 [Cucurbita moschata] >XP_022967135.1 mitotic spindle checkpoint protein MAD2 [Cucurbita maxima] >XP_023534396.1 mitotic spindle checkpoint protein MAD2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.0 bits (1029), Expect = 2.6e-108
Identity = 207/218 (94.95%), Postives = 207/218 (94.95%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MASKTASKDIITLRGSAAIVSEFF           GYAANSILYNRGLYPEESFARVKKY
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFF-----------GYAANSILYNRGLYPEESFARVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE
Sbjct: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE
Sbjct: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 207

BLAST of Carg14280 vs. NCBI nr
Match: XP_023542211.1 (mitotic spindle checkpoint protein MAD2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.8 bits (1026), Expect = 5.8e-108
Identity = 206/218 (94.50%), Postives = 207/218 (94.95%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MASKTASKDIITLRGSAAIVSEFF           GYAANSILYNRGLYPEESFARVKKY
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFF-----------GYAANSILYNRGLYPEESFARVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE
Sbjct: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VV+QGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE
Sbjct: 121 VVDQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 207

BLAST of Carg14280 vs. NCBI nr
Match: XP_022998654.1 (mitotic spindle checkpoint protein MAD2-like [Cucurbita maxima])

HSP 1 Score: 398.3 bits (1022), Expect = 1.7e-107
Identity = 206/218 (94.50%), Postives = 206/218 (94.50%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MASKTASKDIITLRGSAAIVSEFF           GYAANSILYNRGLYPEESFARVKKY
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFF-----------GYAANSILYNRGLYPEESFARVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDG 
Sbjct: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGV 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE
Sbjct: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 207

BLAST of Carg14280 vs. NCBI nr
Match: XP_022944941.1 (mitotic spindle checkpoint protein MAD2-like [Cucurbita moschata])

HSP 1 Score: 396.4 bits (1017), Expect = 6.5e-107
Identity = 206/218 (94.50%), Postives = 206/218 (94.50%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MASKTASKDIITLRGSAAIVSEFF           GYAANSILYNRGLYPEESFARVKKY
Sbjct: 1   MASKTASKDIITLRGSAAIVSEFF-----------GYAANSILYNRGLYPEESFARVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE
Sbjct: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEP VFDVLAYTDKDLAVPFTWIE
Sbjct: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPRVFDVLAYTDKDLAVPFTWIE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 207

BLAST of Carg14280 vs. NCBI nr
Match: XP_004135301.1 (PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis sativus] >KGN51700.1 hypothetical protein Csa_5G590170 [Cucumis sativus])

HSP 1 Score: 391.3 bits (1004), Expect = 2.1e-105
Identity = 202/218 (92.66%), Postives = 205/218 (94.04%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MASKTA+KDIITLRGSAAIVSEFF           GYAANSILYNRGLYPEESF RVKKY
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFF-----------GYAANSILYNRGLYPEESFVRVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDGE
Sbjct: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+E
Sbjct: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWME 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDD DDE
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDDDE 207

BLAST of Carg14280 vs. TAIR10
Match: AT3G25980.1 (DNA-binding HORMA family protein)

HSP 1 Score: 347.8 bits (891), Expect = 4.8e-96
Identity = 179/219 (81.74%), Postives = 194/219 (88.58%), Query Frame = 0

Query: 1   MASKT-ASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKK 60
           MASKT A+KDIITL GSAAIVSEFF            YAANSILYNR +YPEESF +VKK
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFC-----------YAANSILYNRAVYPEESFVKVKK 60

Query: 61  YGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDG 120
           YGLPMLL +DE VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSKA  EVLERWNFRIETD 
Sbjct: 61  YGLPMLLIEDESVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDN 120

Query: 121 EVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWI 180
           EVV++GVSREKSDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTWI
Sbjct: 121 EVVDKGVSREKSDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWI 180

Query: 181 ESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           ESDPKLIANPQMVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 ESDPKLIANPQMVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

BLAST of Carg14280 vs. Swiss-Prot
Match: sp|Q9LU93|MAD2_ARATH (Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 8.6e-95
Identity = 179/219 (81.74%), Postives = 194/219 (88.58%), Query Frame = 0

Query: 1   MASKT-ASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKK 60
           MASKT A+KDIITL GSAAIVSEFF            YAANSILYNR +YPEESF +VKK
Sbjct: 1   MASKTAAAKDIITLHGSAAIVSEFFC-----------YAANSILYNRAVYPEESFVKVKK 60

Query: 61  YGLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDG 120
           YGLPMLL +DE VK+F+SNLT+Q+SEWLEAGKLQRVVLVIMSKA  EVLERWNFRIETD 
Sbjct: 61  YGLPMLLIEDESVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDN 120

Query: 121 EVVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWI 180
           EVV++GVSREKSDKEIMREIQAIMRQ+ASS+TYLPCLDE CVFDVLAYTD D+AVPFTWI
Sbjct: 121 EVVDKGVSREKSDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWI 180

Query: 181 ESDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           ESDPKLIANPQMVKLH FDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 ESDPKLIANPQMVKLHGFDTKIHKVDTLVSYKNDEWDEE 208

BLAST of Carg14280 vs. Swiss-Prot
Match: sp|Q9XFH3|MAD2_MAIZE (Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 1.5e-91
Identity = 173/218 (79.36%), Postives = 189/218 (86.70%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MAS++ASKDIITLRGSAAIVSEFF           GYAANSILYNR +YPEESF++VKKY
Sbjct: 1   MASRSASKDIITLRGSAAIVSEFF-----------GYAANSILYNRAVYPEESFSKVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GL MLLTQDEGVK FI++LT+QLSEWLEAGKLQR+VLVIMSKA +EVLERWNF I TD E
Sbjct: 61  GLTMLLTQDEGVKNFIASLTSQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFNIVTDAE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVE+G  +EKSDKEIMREIQAIMRQIAS ITYLPCLDEPCVFDVLAYTD D+  P TW+E
Sbjct: 121 VVEKGAIKEKSDKEIMREIQAIMRQIASCITYLPCLDEPCVFDVLAYTDTDVDAPGTWVE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SD KLI NPQMVKLHSFDTKIHKVDTLVSYK D+ D+E
Sbjct: 181 SDAKLIDNPQMVKLHSFDTKIHKVDTLVSYKKDEWDEE 207

BLAST of Carg14280 vs. Swiss-Prot
Match: sp|Q556Y9|MD2L1_DICDI (Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX=44689 GN=mad2l1-1 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-54
Identity = 112/212 (52.83%), Postives = 145/212 (68.40%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           M +  ASK  I+L+GS  IV+EFF            Y+ N+IL+ RGLYP ESF RV KY
Sbjct: 1   MQAAVASKTNISLKGSTEIVTEFF-----------SYSINTILFQRGLYPPESFTRVAKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLP+L+T D+ +K ++ N+  QLSEWL +G +Q++VLVI      EVLERW F + TD  
Sbjct: 61  GLPILVTNDQSLKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTD-- 120

Query: 121 VVEQG-VSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWI 180
           + ++G   R+K +KEIM EIQAI+RQI +S+T+LP L   C FD+L YT KDLAVP  W 
Sbjct: 121 IPKEGEAPRQKPEKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWE 180

Query: 181 ESDPKLIANPQMVKLHSFDTKIHKVDTLVSYK 212
           ESDPK I N Q VKL SF T IHKV+++V+YK
Sbjct: 181 ESDPKFITNSQQVKLRSFTTTIHKVESMVAYK 199

BLAST of Carg14280 vs. Swiss-Prot
Match: sp|Q9Z1B5|MD2L1_MOUSE (Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Mad2l1 PE=1 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 5.0e-50
Identity = 103/211 (48.82%), Postives = 140/211 (66.35%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MA + A +  ITLRGSA IV+EFF            +  NSILY RG+YP E+F RV+KY
Sbjct: 1   MAQQLAREQGITLRGSAEIVAEFF-----------SFGINSILYQRGIYPSETFTRVQKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GL +L T D  +  +++N+  QL EWL    +Q++V+VI +  + EVLERW F IE D  
Sbjct: 61  GLTLLTTTDPELIKYLNNVVEQLKEWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKT 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
             E+GV REKS K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W E
Sbjct: 121 AKEEGVRREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYK 212
           S P+ I N + V+L SF T IHKV+++V+YK
Sbjct: 181 SGPQFITNCEEVRLRSFTTTIHKVNSMVAYK 200

BLAST of Carg14280 vs. Swiss-Prot
Match: sp|Q13257|MD2L1_HUMAN (Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD2L1 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-47
Identity = 98/211 (46.45%), Postives = 138/211 (65.40%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MA + + +  ITLRGSA IV+EFF            +  NSILY RG+YP E+F RV+KY
Sbjct: 1   MALQLSREQGITLRGSAEIVAEFF-----------SFGINSILYQRGIYPSETFTRVQKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GL +L+T D  +  +++N+  QL +WL    +Q++V+VI +  + EVLERW F IE D  
Sbjct: 61  GLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKT 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
             +    REKS K I  EI++++RQI +++T+LP L+  C FD+L YTDKDL VP  W E
Sbjct: 121 AKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYK 212
           S P+ I N + V+L SF T IHKV+++V+YK
Sbjct: 181 SGPQFITNSEEVRLRSFTTTIHKVNSMVAYK 200

BLAST of Carg14280 vs. TrEMBL
Match: tr|A0A0A0KPY5|A0A0A0KPY5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-105
Identity = 202/218 (92.66%), Postives = 205/218 (94.04%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MASKTA+KDIITLRGSAAIVSEFF           GYAANSILYNRGLYPEESF RVKKY
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFF-----------GYAANSILYNRGLYPEESFVRVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDGE
Sbjct: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+E
Sbjct: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWME 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDD DDE
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDDDE 207

BLAST of Carg14280 vs. TrEMBL
Match: tr|A0A1S3BDR9|A0A1S3BDR9_CUCME (mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 5.8e-104
Identity = 199/218 (91.28%), Postives = 204/218 (93.58%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MASKTA+KDIITLRGSAAIVSEFF           GYAANSILYNRGLYPEESF RVKKY
Sbjct: 1   MASKTATKDIITLRGSAAIVSEFF-----------GYAANSILYNRGLYPEESFVRVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVK+FISNLTAQLSEWLEAGKLQRVVLVIMSK+NNEVLERWNFRIETDGE
Sbjct: 61  GLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLVIMSKSNNEVLERWNFRIETDGE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTW+E
Sbjct: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWME 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDD  D+
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDDGDD 207

BLAST of Carg14280 vs. TrEMBL
Match: tr|A0A1R3IFP4|A0A1R3IFP4_9ROSI (DNA-binding HORMA OS=Corchorus olitorius OX=93759 GN=COLO4_23607 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.0e-100
Identity = 192/218 (88.07%), Postives = 202/218 (92.66%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MAS+T +KDIITLRGSAAIVSEFF           GYAANSILYNRG+YPEESF++VKKY
Sbjct: 1   MASRTVAKDIITLRGSAAIVSEFF-----------GYAANSILYNRGVYPEESFSKVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVK+FISNLTAQLSEWLEAGKLQRVVLVIMSKA NEVLERWNF IETD E
Sbjct: 61  GLPMLLTQDEGVKSFISNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDNE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVE+GVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+AVPFTWIE
Sbjct: 121 VVEKGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKND+ D+E
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDEWDEE 207

BLAST of Carg14280 vs. TrEMBL
Match: tr|A0A061E776|A0A061E776_THECC (DNA-binding HORMA family protein OS=Theobroma cacao OX=3641 GN=TCM_010752 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.7e-100
Identity = 191/218 (87.61%), Postives = 202/218 (92.66%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MAS+TA+KDIITLRGSAAIVSEFF           GYAANSILYNRG+YPEESF +VKKY
Sbjct: 1   MASRTAAKDIITLRGSAAIVSEFF-----------GYAANSILYNRGVYPEESFVKVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLT+DEGVK+FI+NLTAQLSEWLEAGKLQRVVLVIMSKA NEVLERWNF IETD E
Sbjct: 61  GLPMLLTEDEGVKSFITNLTAQLSEWLEAGKLQRVVLVIMSKATNEVLERWNFSIETDSE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVE+GVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKD+AVPFTWIE
Sbjct: 121 VVEKGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDVAVPFTWIE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDDE 219
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDD D++
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDWDEQ 207

BLAST of Carg14280 vs. TrEMBL
Match: tr|A0A2G9I9H0|A0A2G9I9H0_9LAMI (Spindle assembly checkpoint protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_00861 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.3e-100
Identity = 191/217 (88.02%), Postives = 201/217 (92.63%), Query Frame = 0

Query: 1   MASKTASKDIITLRGSAAIVSEFFVLFRCPEIHHLGYAANSILYNRGLYPEESFARVKKY 60
           MAS+TA+KDIITLRGSAAIVSEFF           GYAANSILYNRG+YPEESFA+VKKY
Sbjct: 1   MASRTAAKDIITLRGSAAIVSEFF-----------GYAANSILYNRGVYPEESFAKVKKY 60

Query: 61  GLPMLLTQDEGVKTFISNLTAQLSEWLEAGKLQRVVLVIMSKANNEVLERWNFRIETDGE 120
           GLPMLLTQDEGVK+FISNLT+QLSEWLEAGKLQRVVLVIMSKA  EVLERWNF IETDGE
Sbjct: 61  GLPMLLTQDEGVKSFISNLTSQLSEWLEAGKLQRVVLVIMSKATGEVLERWNFSIETDGE 120

Query: 121 VVEQGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCVFDVLAYTDKDLAVPFTWIE 180
           VVE+GVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPC+FDVLAYTDKD AVPFTWIE
Sbjct: 121 VVEKGVSREKSDKEIMREIQAIMRQIASSITYLPCLDEPCIFDVLAYTDKDCAVPFTWIE 180

Query: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDDCDD 218
           SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKND+ D+
Sbjct: 181 SDPKLIANPQMVKLHSFDTKIHKVDTLVSYKNDEWDE 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022956917.12.6e-10894.95mitotic spindle checkpoint protein MAD2 [Cucurbita moschata] >XP_022967135.1 mit... [more]
XP_023542211.15.8e-10894.50mitotic spindle checkpoint protein MAD2 [Cucurbita pepo subsp. pepo][more]
XP_022998654.11.7e-10794.50mitotic spindle checkpoint protein MAD2-like [Cucurbita maxima][more]
XP_022944941.16.5e-10794.50mitotic spindle checkpoint protein MAD2-like [Cucurbita moschata][more]
XP_004135301.12.1e-10592.66PREDICTED: mitotic spindle checkpoint protein MAD2 [Cucumis sativus] >KGN51700.1... [more]
Match NameE-valueIdentityDescription
AT3G25980.14.8e-9681.74DNA-binding HORMA family protein[more]
Match NameE-valueIdentityDescription
sp|Q9LU93|MAD2_ARATH8.6e-9581.74Mitotic spindle checkpoint protein MAD2 OS=Arabidopsis thaliana OX=3702 GN=MAD2 ... [more]
sp|Q9XFH3|MAD2_MAIZE1.5e-9179.36Mitotic spindle checkpoint protein MAD2 OS=Zea mays OX=4577 GN=MAD2 PE=2 SV=1[more]
sp|Q556Y9|MD2L1_DICDI1.5e-5452.83Mitotic spindle assembly checkpoint protein MAD2A OS=Dictyostelium discoideum OX... [more]
sp|Q9Z1B5|MD2L1_MOUSE5.0e-5048.82Mitotic spindle assembly checkpoint protein MAD2A OS=Mus musculus OX=10090 GN=Ma... [more]
sp|Q13257|MD2L1_HUMAN1.3e-4746.45Mitotic spindle assembly checkpoint protein MAD2A OS=Homo sapiens OX=9606 GN=MAD... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KPY5|A0A0A0KPY5_CUCSA1.4e-10592.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G590170 PE=4 SV=1[more]
tr|A0A1S3BDR9|A0A1S3BDR9_CUCME5.8e-10491.28mitotic spindle checkpoint protein MAD2 OS=Cucumis melo OX=3656 GN=LOC103488916 ... [more]
tr|A0A1R3IFP4|A0A1R3IFP4_9ROSI1.0e-10088.07DNA-binding HORMA OS=Corchorus olitorius OX=93759 GN=COLO4_23607 PE=4 SV=1[more]
tr|A0A061E776|A0A061E776_THECC1.7e-10087.61DNA-binding HORMA family protein OS=Theobroma cacao OX=3641 GN=TCM_010752 PE=4 S... [more]
tr|A0A2G9I9H0|A0A2G9I9H0_9LAMI2.3e-10088.02Spindle assembly checkpoint protein OS=Handroanthus impetiginosus OX=429701 GN=C... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0007094mitotic spindle assembly checkpoint
Vocabulary: INTERPRO
TermDefinition
IPR027097Mad2
IPR003511HORMA_dom
IPR036570HORMA_dom_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007094 mitotic spindle assembly checkpoint
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14280-RACarg14280-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036570HORMA domain superfamilyGENE3DG3DSA:3.30.900.10coord: 2..216
e-value: 6.8E-71
score: 239.9
IPR036570HORMA domain superfamilySUPERFAMILYSSF56019The spindle assembly checkpoint protein mad2coord: 10..215
IPR003511HORMA domainPFAMPF02301HORMAcoord: 35..202
e-value: 7.1E-18
score: 64.9
IPR003511HORMA domainPROSITEPS50815HORMAcoord: 25..208
score: 40.141
IPR027097Mitotic spindle checkpoint protein Mad2PANTHERPTHR11842:SF11MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2Acoord: 1..213
NoneNo IPR availablePANTHERPTHR11842MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2coord: 1..213

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg14280Carg21251Silver-seed gourdcarcarB285