CmaCh03G001010 (gene) Cucurbita maxima (Rimu)

NameCmaCh03G001010
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionE3 ubiquitin-protein ligase
LocationCma_Chr03 : 1054954 .. 1056707 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAAATCAGAGAATAATTCTACATCTTCAAAAGTCCTACTTGGTTCGAGTGGGAAGCTTGGAGTTTATTCAACCAATGGCGTGCAAGAATTGCTCGAGTGTCCTGTCTGCACAAATCTGATGTACCCTCCAATTTACCAGGTATGTACCTTATATCCTGTTTTTCCTCTTGTACTTCTCGTTATAAATTAAGCTATGCTCGGGAGTGTTCGGAGTGCTTTCAGCGTGGAAAAAAGTACTTTTTCTGTATGGCTTAATTTCTTAATTCTTGATTCAAAGGTTGATTTGGACTCTGTACCGTGGCGAATTCTCCATGTTGCTGGTTCTTTAGTTCTTTTCTTAGTATTAAGGTTGTATTGAAGACATAGAACATCTCTTTATTCTTGAATCTTTACGTCGTGGAGGTGTGATATATTTTTCCGCTTCTGCTTTGTTTCGGACTTTCTTGACTGGCTTTTGAACTTTAGTGCCCAAACGGCCACACACTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGCTGCCCGACCTGTCGTCATGAACTCGGAAACATAAGGTGCTTGGCATTGGAGAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAGATACCAGAATTTGGGTTGCCAAGATATTTTCCCTTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAACTGCCCTTATGCTGGATCCGAGTGCTCGGTCACGGGCGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCACGACGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAACGCCACATGGATGCTAACGGTAGGTGCTTGCTCTATATAGAATCAGTATACTTTCTCACCATGCTTCACTTCACATAACTTAACTATGTAAGCTATATATACAAGTTTTCTTGAAAATCAAATGTATAGTTAAATGGTTCGTCCCGTGAGTATATATAAATATATATTTCGATAAACCGTGTAATCTGAACGATGATAATTGCTAGCTAAAGGTCTGAAAAACCGGGTTGTCTTCCATCTTTGAGCATTGGAATATTTTATAGTAGCTTGCAATGGCAGGTTTTCAACTGCTTTGGAAGACAGTTCTGCTTGCATTTCGAGGCGTTCCAGCTAGGAACAGCGCCTGTTTACATGGCGTTCTTACGATTCATGGGCGAAGATAACGAAGCAAAAAAGTTCAGCTACAGTTTGGAAGTGGGTCACAACGGGCGTAAACTGATATGGCAAGGAATTCCGAGAGGCATCCGTGACAGTCACCGAAAAGTTCGTGATAGTCAAGACGGTCTCATCATTCAAAGAAATCTGGCACTGTATTTTTCGGGGGGAGAAAGACGAGAGCTGAAACTGAGAATCACGGGTCGAATATGGAAAGAAGAATGAGAGCAGGAGCAAGGGCCTAAGCTAAGCTCTTGTGGTGTTCAGGTGAATGTATCATTGTACAGGTAAAACAAGTTTGTTCTCCAAGTGCTGAAAAAGCTCTTGGAGAAACTTCCAAGCTAATATAAAAAATGTAACCTACAAAATGAATCAAAGAAGCTTTTATGTATTTAAAGCCAAGTTTCTCTTAAGTCTTTCTTTTGTTAGTATGATTCATTAGCTCTGCATTTCAAATTGTTCGTTTGGCAGGTGTTTTATGGCTGTCCATGGAATGGAGTTTTGGCACTTGGAAATATTAAAATTACTAATCGGTCCTTAT

mRNA sequence

ATGTCTAAATCAGAGAATAATTCTACATCTTCAAAAGTCCTACTTGGTTCGAGTGGGAAGCTTGGAGTTTATTCAACCAATGGCGTGCAAGAATTGCTCGAGTGTCCTGTCTGCACAAATCTGATGTACCCTCCAATTTACCAGTGCCCAAACGGCCACACACTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGCTGCCCGACCTGTCGTCATGAACTCGGAAACATAAGGTGCTTGGCATTGGAGAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAGATACCAGAATTTGGGTTGCCAAGATATTTTCCCTTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAACTGCCCTTATGCTGGATCCGAGTGCTCGGTCACGGGCGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCACGACGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAACGCCACATGGATGCTAACGGTTTTCAACTGCTTTGGAAGACAGTTCTGCTTGCATTTCGAGGCGTTCCAGCTAGGAACAGCGCCTGTTTACATGGCGTTCTTACGATTCATGGGCGAAGATAACGAAGCAAAAAAGTTCAGCTACAGTTTGGAAGTGGGTCACAACGGGCGTAAACTGATATGGCAAGGAATTCCGAGAGGCATCCGTGACAGTCACCGAAAAGTTCGTGATAGTCAAGACGGTCTCATCATTCAAAGAAATCTGGCACTGTATTTTTCGGGGGGAGAAAGACGAGAGCTGAAACTGAGAATCACGGGTCGAATATGGAAAGAAGAATGAGAGCAGGAGCAAGGGCCTAAGCTAAGCTCTTGTGGTGTTCAGGTGAATGTATCATTGTACAGGTAAAACAAGTTTGTTCTCCAAGTGCTGAAAAAGCTCTTGGAGAAACTTCCAAGCTAATATAAAAAATGTAACCTACAAAATGAATCAAAGAAGCTTTTATGTATTTAAAGCCAAGTTTCTCTTAAGTCTTTCTTTTGTTAGTATGATTCATTAGCTCTGCATTTCAAATTGTTCGTTTGGCAGGTGTTTTATGGCTGTCCATGGAATGGAGTTTTGGCACTTGGAAATATTAAAATTACTAATCGGTCCTTAT

Coding sequence (CDS)

ATGTCTAAATCAGAGAATAATTCTACATCTTCAAAAGTCCTACTTGGTTCGAGTGGGAAGCTTGGAGTTTATTCAACCAATGGCGTGCAAGAATTGCTCGAGTGTCCTGTCTGCACAAATCTGATGTACCCTCCAATTTACCAGTGCCCAAACGGCCACACACTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGCTGCCCGACCTGTCGTCATGAACTCGGAAACATAAGGTGCTTGGCATTGGAGAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAGATACCAGAATTTGGGTTGCCAAGATATTTTCCCTTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAACTGCCCTTATGCTGGATCCGAGTGCTCGGTCACGGGCGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCACGACGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCACACGAAGTTGAAAACGCCACATGGATGCTAACGGTTTTCAACTGCTTTGGAAGACAGTTCTGCTTGCATTTCGAGGCGTTCCAGCTAGGAACAGCGCCTGTTTACATGGCGTTCTTACGATTCATGGGCGAAGATAACGAAGCAAAAAAGTTCAGCTACAGTTTGGAAGTGGGTCACAACGGGCGTAAACTGATATGGCAAGGAATTCCGAGAGGCATCCGTGACAGTCACCGAAAAGTTCGTGATAGTCAAGACGGTCTCATCATTCAAAGAAATCTGGCACTGTATTTTTCGGGGGGAGAAAGACGAGAGCTGAAACTGAGAATCACGGGTCGAATATGGAAAGAAGAATGA

Protein sequence

MSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE
BLAST of CmaCh03G001010 vs. Swiss-Prot
Match: SINA2_ARATH (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 2.4e-150
Identity = 242/278 (87.05%), Postives = 257/278 (92.45%), Query Frame = 1

Query: 6   NNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN 65
           NN+  +K      GK G++S NGV ELLECPVCTNLMYPPI+QCPNGHTLCSNCK+RV N
Sbjct: 31  NNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQN 90

Query: 66  CCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPY 125
            CPTCR+ELGNIRCLALEKVAESLE+PCRYQNLGC DIFPYYSKLKHEQHCRFRPY CPY
Sbjct: 91  TCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPY 150

Query: 126 AGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ 185
           AGSECSVTGDIP+LV HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ
Sbjct: 151 AGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ 210

Query: 186 FCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRK 245
           FCLHFEAFQLG APVYMAFLRFMG++NEAKKFSYSLEVG +GRKL WQGIPR IRDSHRK
Sbjct: 211 FCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRK 270

Query: 246 VRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           VRDSQDGLII RNLALYFSGG+R+ELKLR+TGRIWKEE
Sbjct: 271 VRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of CmaCh03G001010 vs. Swiss-Prot
Match: SINA1_ARATH (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 4.3e-147
Identity = 242/283 (85.51%), Postives = 259/283 (91.52%), Query Frame = 1

Query: 1   MSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCK 60
           M+K E NS  +K   GS GK   +S+NGV ELLECPVCTNLMYPPI+QCPNGHTLCS+CK
Sbjct: 25  MAKVEANSKPTKSGSGSIGKF--HSSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCK 84

Query: 61  IRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRP 120
           +RV N CPTCR+ELGNIRCLALEKVAESLE+PCRYQNLGCQDIFPYYSKLKHEQHCRFR 
Sbjct: 85  LRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRS 144

Query: 121 YNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFN 180
           Y+CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFN
Sbjct: 145 YSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFN 204

Query: 181 CFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIR 240
           CFGRQFCLHFEAFQLG APVYMAFLRFMG++NEAKKFSYSLEVG + RKL WQGIPR IR
Sbjct: 205 CFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIR 264

Query: 241 DSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           DSHRKVRDSQDGLII RNLALYFSG ++ ELKLR+TGRIWKEE
Sbjct: 265 DSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

BLAST of CmaCh03G001010 vs. Swiss-Prot
Match: SINA3_ARATH (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 2.8e-130
Identity = 212/277 (76.53%), Postives = 241/277 (87.00%), Query Frame = 1

Query: 7   NSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNC 66
           N+ ++ V   +SG L   +T  V ELLECPVCTN MYPPI+QC NGHTLCS CK RV N 
Sbjct: 37  NNNTTNVNAAASGLLP--TTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNR 96

Query: 67  CPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYA 126
           CPTCR ELG+IRCLALEKVAESLE+PC++ +LGC +IFPYYSKLKHE  C FRPY+CPYA
Sbjct: 97  CPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYA 156

Query: 127 GSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQF 186
           GSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ F
Sbjct: 157 GSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYF 216

Query: 187 CLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKV 246
           CLHFEAFQLG APVYMAFLRFMG++ EA+ ++YSLEVG  GRKLIW+G PR +RDSHRKV
Sbjct: 217 CLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKV 276

Query: 247 RDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           RDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 277 RDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

BLAST of CmaCh03G001010 vs. Swiss-Prot
Match: SINA4_ARATH (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 3.2e-126
Identity = 203/282 (71.99%), Postives = 235/282 (83.33%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           +K+   + ++ V+    G         V ELLECPVCT  MYPPI+QC NGHTLCS CK+
Sbjct: 31  TKTHGGAAAAAVVTNIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKV 90

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV N CPTCR ELG+IRCLALEKVAESLE+PC++ NLGC +IFPYYSKLKHE  C FRPY
Sbjct: 91  RVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPY 150

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSEC + GDIP LVAHL+DDHKVDMH G TFNHRYVKSNP EVENATWMLTVF+C
Sbjct: 151 SCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHC 210

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FG+ FCLHFEAFQLG  PVYMAFLRFMG++ +A+ +SYSLEVG +GRKL W+G PR IRD
Sbjct: 211 FGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRD 270

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TG+IWKE+
Sbjct: 271 SHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of CmaCh03G001010 vs. Swiss-Prot
Match: SINA5_ARATH (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 452.2 bits (1162), Expect = 4.1e-126
Identity = 200/255 (78.43%), Postives = 225/255 (88.24%), Query Frame = 1

Query: 29  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES 88
           V ELLECPVCTN MYPPI+QC NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 40  VYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAES 99

Query: 89  LEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHK 148
           LE+PC+Y NLGC  IFPYYSKLKHE  C FRPY+CPYAGSEC+  GDI  LVAHL+DDHK
Sbjct: 100 LELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHK 159

Query: 149 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFM 208
           VDMH GCTFNHRYVKSNP EVENATWMLTVF CFG+ FCLHFEAFQLG APVYMAFLRFM
Sbjct: 160 VDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFM 219

Query: 209 GEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 268
           G++++A+ ++YSLEVG +GRK  W+G PR +RDSHRKVRDS DGLIIQRN+AL+FSGG++
Sbjct: 220 GDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDK 279

Query: 269 RELKLRITGRIWKEE 284
           +ELKLR+TGRIWKE+
Sbjct: 280 KELKLRVTGRIWKEQ 294

BLAST of CmaCh03G001010 vs. TrEMBL
Match: B9HA10_POPTR (E3 ubiquitin-protein ligase OS=Populus trichocarpa GN=POPTR_0006s208602g PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 1.4e-152
Identity = 248/282 (87.94%), Postives = 267/282 (94.68%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           SKSENN T +K  +   GK GVYS NGV ELLECPVCTNLMYPPI+QCPNGHTLCSNCK+
Sbjct: 26  SKSENNITPTKGTVVLGGKHGVYSNNGVHELLECPVCTNLMYPPIHQCPNGHTLCSNCKL 85

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV NCCPTCR++LGNIRCLALEKVAESLE+PC++Q+LGC DIFPYYSKLKHEQHCRFRPY
Sbjct: 86  RVHNCCPTCRYDLGNIRCLALEKVAESLELPCKFQSLGCLDIFPYYSKLKHEQHCRFRPY 145

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSECSVTGDIP+LV+HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC
Sbjct: 146 SCPYAGSECSVTGDIPALVSHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 205

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKL+WQGIPR IRD
Sbjct: 206 FGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLVWQGIPRSIRD 265

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDSQDGLIIQRNLALYFSGG+R+ELKLR+TGR+WKEE
Sbjct: 266 SHRKVRDSQDGLIIQRNLALYFSGGDRQELKLRVTGRVWKEE 307

BLAST of CmaCh03G001010 vs. TrEMBL
Match: B9T806_RICCO (E3 ubiquitin-protein ligase OS=Ricinus communis GN=RCOM_0446280 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 5.2e-152
Identity = 249/282 (88.30%), Postives = 267/282 (94.68%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           +K+ENNST +K   G  GK G+YS NGV ELLECPVCTNLMYPPI+QCPNGHTLCSNCK 
Sbjct: 26  AKTENNSTVTK---GLGGKQGLYSNNGVHELLECPVCTNLMYPPIHQCPNGHTLCSNCKS 85

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV NCCPTCR++LGNIRCLALEKVAESLE+PC+YQ+LGC DIFPYYSKLKHEQHCRFRPY
Sbjct: 86  RVHNCCPTCRYDLGNIRCLALEKVAESLELPCKYQDLGCHDIFPYYSKLKHEQHCRFRPY 145

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSECS+TGDIP+LVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC
Sbjct: 146 SCPYAGSECSITGDIPTLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 205

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FGRQFCLHFEAFQ+G APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKLIWQGIPR IRD
Sbjct: 206 FGRQFCLHFEAFQIGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRSIRD 265

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDSQDGLIIQR+LALYFSGG+R+ELKLRITGRIWKEE
Sbjct: 266 SHRKVRDSQDGLIIQRSLALYFSGGDRQELKLRITGRIWKEE 304

BLAST of CmaCh03G001010 vs. TrEMBL
Match: A0A0D2QH79_GOSRA (E3 ubiquitin-protein ligase OS=Gossypium raimondii GN=B456_002G223600 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 6.8e-152
Identity = 249/289 (86.16%), Postives = 269/289 (93.08%), Query Frame = 1

Query: 2   SKSENNSTSS-------KVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHT 61
           +KSE+N+ +S       K  +   GK G++S NGV ELLECPVCTNLMYPPI+QCPNGHT
Sbjct: 26  AKSESNNATSTTTTATMKTSVNLVGKHGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHT 85

Query: 62  LCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQ 121
           LCSNCKIRV NCCPTCR++LGNIRCLALEKVAESLE+PC+YQNLGCQDIFPYYSKLKHEQ
Sbjct: 86  LCSNCKIRVHNCCPTCRYDLGNIRCLALEKVAESLELPCKYQNLGCQDIFPYYSKLKHEQ 145

Query: 122 HCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATW 181
           HCRFRPYNCPYAGS+CSVTGDIP+LV+HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATW
Sbjct: 146 HCRFRPYNCPYAGSDCSVTGDIPTLVSHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATW 205

Query: 182 MLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQG 241
           MLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKLIWQG
Sbjct: 206 MLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGANGRKLIWQG 265

Query: 242 IPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           IPR IRDSHRKVRDSQDGL+IQRNLALYFSGG+R+ELKLR+TGRIWKEE
Sbjct: 266 IPRSIRDSHRKVRDSQDGLVIQRNLALYFSGGDRQELKLRVTGRIWKEE 314

BLAST of CmaCh03G001010 vs. TrEMBL
Match: B9IIA7_POPTR (E3 ubiquitin-protein ligase OS=Populus trichocarpa GN=POPTR_0016s05970g PE=3 SV=2)

HSP 1 Score: 544.3 bits (1401), Expect = 8.9e-152
Identity = 247/282 (87.59%), Postives = 265/282 (93.97%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           SKSENN   +K  +  SGK GVYS NGV ELLECPVCTNLMYPPI+QCPNGHTLCS CK+
Sbjct: 23  SKSENNIAPTKGTVILSGKHGVYSNNGVHELLECPVCTNLMYPPIHQCPNGHTLCSACKL 82

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV NCCPTCR++LGNIRCLALEKVAESLE+PC+YQ+LGC D+FPYYSKLKHEQHCRFRPY
Sbjct: 83  RVHNCCPTCRYDLGNIRCLALEKVAESLELPCKYQSLGCLDVFPYYSKLKHEQHCRFRPY 142

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSECSVTGDIP+L AHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC
Sbjct: 143 SCPYAGSECSVTGDIPALAAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 202

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKL+WQGIPR IRD
Sbjct: 203 FGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLVWQGIPRSIRD 262

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDSQDGLIIQRNLALYFSGG+R+ELKLR+TGR+WKEE
Sbjct: 263 SHRKVRDSQDGLIIQRNLALYFSGGDRKELKLRVTGRVWKEE 304

BLAST of CmaCh03G001010 vs. TrEMBL
Match: A0A067JYP2_JATCU (E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_25565 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 2.0e-151
Identity = 248/282 (87.94%), Postives = 264/282 (93.62%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           +K ENN T++K      GK GVY+ NGV ELLECPVC+NLMYPPI+QCPNGHTLCSNCKI
Sbjct: 26  AKVENNITTAKGAPSLGGKHGVYTNNGVHELLECPVCSNLMYPPIHQCPNGHTLCSNCKI 85

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV NCCPTCR+ELGNIRCLALEKVAESLE+PC+YQ+LGC DIFPYYSKLKHEQHCRFRPY
Sbjct: 86  RVHNCCPTCRYELGNIRCLALEKVAESLELPCKYQDLGCHDIFPYYSKLKHEQHCRFRPY 145

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
            CPYAGSECSVTGDIP+LVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC
Sbjct: 146 TCPYAGSECSVTGDIPALVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 205

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKL WQGIPR IRD
Sbjct: 206 FGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLTWQGIPRSIRD 265

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDSQDGLIIQR+LALYFSGG+R+ELKLR+TGRIWKEE
Sbjct: 266 SHRKVRDSQDGLIIQRSLALYFSGGDRQELKLRVTGRIWKEE 307

BLAST of CmaCh03G001010 vs. TAIR10
Match: AT3G58040.1 (AT3G58040.1 seven in absentia of Arabidopsis 2)

HSP 1 Score: 532.7 bits (1371), Expect = 1.4e-151
Identity = 242/278 (87.05%), Postives = 257/278 (92.45%), Query Frame = 1

Query: 6   NNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSN 65
           NN+  +K      GK G++S NGV ELLECPVCTNLMYPPI+QCPNGHTLCSNCK+RV N
Sbjct: 31  NNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQN 90

Query: 66  CCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPY 125
            CPTCR+ELGNIRCLALEKVAESLE+PCRYQNLGC DIFPYYSKLKHEQHCRFRPY CPY
Sbjct: 91  TCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPY 150

Query: 126 AGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ 185
           AGSECSVTGDIP+LV HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ
Sbjct: 151 AGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQ 210

Query: 186 FCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRK 245
           FCLHFEAFQLG APVYMAFLRFMG++NEAKKFSYSLEVG +GRKL WQGIPR IRDSHRK
Sbjct: 211 FCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRK 270

Query: 246 VRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           VRDSQDGLII RNLALYFSGG+R+ELKLR+TGRIWKEE
Sbjct: 271 VRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of CmaCh03G001010 vs. TAIR10
Match: AT2G41980.1 (AT2G41980.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 521.9 bits (1343), Expect = 2.4e-148
Identity = 242/283 (85.51%), Postives = 259/283 (91.52%), Query Frame = 1

Query: 1   MSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCK 60
           M+K E NS  +K   GS GK   +S+NGV ELLECPVCTNLMYPPI+QCPNGHTLCS+CK
Sbjct: 25  MAKVEANSKPTKSGSGSIGKF--HSSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCK 84

Query: 61  IRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRP 120
           +RV N CPTCR+ELGNIRCLALEKVAESLE+PCRYQNLGCQDIFPYYSKLKHEQHCRFR 
Sbjct: 85  LRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRS 144

Query: 121 YNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFN 180
           Y+CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFN
Sbjct: 145 YSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFN 204

Query: 181 CFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIR 240
           CFGRQFCLHFEAFQLG APVYMAFLRFMG++NEAKKFSYSLEVG + RKL WQGIPR IR
Sbjct: 205 CFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIR 264

Query: 241 DSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           DSHRKVRDSQDGLII RNLALYFSG ++ ELKLR+TGRIWKEE
Sbjct: 265 DSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWKEE 305

BLAST of CmaCh03G001010 vs. TAIR10
Match: AT3G61790.1 (AT3G61790.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 466.1 bits (1198), Expect = 1.6e-131
Identity = 212/277 (76.53%), Postives = 241/277 (87.00%), Query Frame = 1

Query: 7   NSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNC 66
           N+ ++ V   +SG L   +T  V ELLECPVCTN MYPPI+QC NGHTLCS CK RV N 
Sbjct: 37  NNNTTNVNAAASGLLP--TTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNR 96

Query: 67  CPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYA 126
           CPTCR ELG+IRCLALEKVAESLE+PC++ +LGC +IFPYYSKLKHE  C FRPY+CPYA
Sbjct: 97  CPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYA 156

Query: 127 GSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQF 186
           GSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ F
Sbjct: 157 GSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYF 216

Query: 187 CLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKV 246
           CLHFEAFQLG APVYMAFLRFMG++ EA+ ++YSLEVG  GRKLIW+G PR +RDSHRKV
Sbjct: 217 CLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKV 276

Query: 247 RDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           RDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 277 RDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

BLAST of CmaCh03G001010 vs. TAIR10
Match: AT4G27880.1 (AT4G27880.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 452.6 bits (1163), Expect = 1.8e-127
Identity = 203/282 (71.99%), Postives = 235/282 (83.33%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           +K+   + ++ V+    G         V ELLECPVCT  MYPPI+QC NGHTLCS CK+
Sbjct: 31  TKTHGGAAAAAVVTNIVGPTATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKV 90

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV N CPTCR ELG+IRCLALEKVAESLE+PC++ NLGC +IFPYYSKLKHE  C FRPY
Sbjct: 91  RVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPY 150

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSEC + GDIP LVAHL+DDHKVDMH G TFNHRYVKSNP EVENATWMLTVF+C
Sbjct: 151 SCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHC 210

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FG+ FCLHFEAFQLG  PVYMAFLRFMG++ +A+ +SYSLEVG +GRKL W+G PR IRD
Sbjct: 211 FGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRD 270

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TG+IWKE+
Sbjct: 271 SHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIWKEQ 312

BLAST of CmaCh03G001010 vs. TAIR10
Match: AT5G53360.1 (AT5G53360.1 TRAF-like superfamily protein)

HSP 1 Score: 360.9 bits (925), Expect = 7.1e-100
Identity = 158/204 (77.45%), Postives = 182/204 (89.22%), Query Frame = 1

Query: 80  LALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSL 139
           +ALEKVAESLE+PC+Y NLGC  IFPYYSKLKHE  C FRPY+CPYAGSEC+  GDI  L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 140 VAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAP 199
           VAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF CFG+ FCLHFEAFQLG AP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 200 VYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNL 259
           VYMAFLRFMG++++A+ ++YSLEVG +GRK  W+G PR +RDSHRKVRDS DGLIIQRN+
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 260 ALYFSGGERRELKLRITGRIWKEE 284
           AL+FSGG+++ELKLR+TGRIWKE+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

BLAST of CmaCh03G001010 vs. NCBI nr
Match: gi|224088178|ref|XP_002308357.1| (Ubiquitin ligase SINAT2 family protein [Populus trichocarpa])

HSP 1 Score: 547.0 bits (1408), Expect = 2.0e-152
Identity = 248/282 (87.94%), Postives = 267/282 (94.68%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           SKSENN T +K  +   GK GVYS NGV ELLECPVCTNLMYPPI+QCPNGHTLCSNCK+
Sbjct: 26  SKSENNITPTKGTVVLGGKHGVYSNNGVHELLECPVCTNLMYPPIHQCPNGHTLCSNCKL 85

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV NCCPTCR++LGNIRCLALEKVAESLE+PC++Q+LGC DIFPYYSKLKHEQHCRFRPY
Sbjct: 86  RVHNCCPTCRYDLGNIRCLALEKVAESLELPCKFQSLGCLDIFPYYSKLKHEQHCRFRPY 145

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSECSVTGDIP+LV+HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC
Sbjct: 146 SCPYAGSECSVTGDIPALVSHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 205

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKL+WQGIPR IRD
Sbjct: 206 FGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLVWQGIPRSIRD 265

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDSQDGLIIQRNLALYFSGG+R+ELKLR+TGR+WKEE
Sbjct: 266 SHRKVRDSQDGLIIQRNLALYFSGGDRQELKLRVTGRVWKEE 307

BLAST of CmaCh03G001010 vs. NCBI nr
Match: gi|743922429|ref|XP_011005289.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Populus euphratica])

HSP 1 Score: 546.6 bits (1407), Expect = 2.6e-152
Identity = 251/284 (88.38%), Postives = 270/284 (95.07%), Query Frame = 1

Query: 2   SKSENNSTSSK--VLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNC 61
           SKSENN T +K  V+LG  GK GVYS NGV ELLECPVCTNLMYPPI+QCPNGHTLCSNC
Sbjct: 26  SKSENNITPTKGTVVLGG-GKHGVYSNNGVHELLECPVCTNLMYPPIHQCPNGHTLCSNC 85

Query: 62  KIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFR 121
           K+RV NCCPTCR++LGNIRCLALEKVAESLE+PC++Q+LGC DIFPYYSKLKHEQHCRFR
Sbjct: 86  KLRVHNCCPTCRYDLGNIRCLALEKVAESLELPCKFQSLGCLDIFPYYSKLKHEQHCRFR 145

Query: 122 PYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVF 181
           PY+CPYAGSECSVTGDIP+LV+HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVF
Sbjct: 146 PYSCPYAGSECSVTGDIPALVSHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVF 205

Query: 182 NCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGI 241
           NCFGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKL+WQGIPR I
Sbjct: 206 NCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLVWQGIPRSI 265

Query: 242 RDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           RDSHRKVRDSQDGLIIQRNLALYFSGG+R+ELKLR+TGR+WKEE
Sbjct: 266 RDSHRKVRDSQDGLIIQRNLALYFSGGDRQELKLRVTGRVWKEE 308

BLAST of CmaCh03G001010 vs. NCBI nr
Match: gi|255587731|ref|XP_002534375.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Ricinus communis])

HSP 1 Score: 545.0 bits (1403), Expect = 7.5e-152
Identity = 249/282 (88.30%), Postives = 267/282 (94.68%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           +K+ENNST +K   G  GK G+YS NGV ELLECPVCTNLMYPPI+QCPNGHTLCSNCK 
Sbjct: 26  AKTENNSTVTK---GLGGKQGLYSNNGVHELLECPVCTNLMYPPIHQCPNGHTLCSNCKS 85

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV NCCPTCR++LGNIRCLALEKVAESLE+PC+YQ+LGC DIFPYYSKLKHEQHCRFRPY
Sbjct: 86  RVHNCCPTCRYDLGNIRCLALEKVAESLELPCKYQDLGCHDIFPYYSKLKHEQHCRFRPY 145

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSECS+TGDIP+LVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC
Sbjct: 146 SCPYAGSECSITGDIPTLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 205

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FGRQFCLHFEAFQ+G APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKLIWQGIPR IRD
Sbjct: 206 FGRQFCLHFEAFQIGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLIWQGIPRSIRD 265

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDSQDGLIIQR+LALYFSGG+R+ELKLRITGRIWKEE
Sbjct: 266 SHRKVRDSQDGLIIQRSLALYFSGGDRQELKLRITGRIWKEE 304

BLAST of CmaCh03G001010 vs. NCBI nr
Match: gi|823136405|ref|XP_012467963.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Gossypium raimondii])

HSP 1 Score: 544.7 bits (1402), Expect = 9.8e-152
Identity = 249/289 (86.16%), Postives = 269/289 (93.08%), Query Frame = 1

Query: 2   SKSENNSTSS-------KVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHT 61
           +KSE+N+ +S       K  +   GK G++S NGV ELLECPVCTNLMYPPI+QCPNGHT
Sbjct: 26  AKSESNNATSTTTTATMKTSVNLVGKHGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHT 85

Query: 62  LCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQ 121
           LCSNCKIRV NCCPTCR++LGNIRCLALEKVAESLE+PC+YQNLGCQDIFPYYSKLKHEQ
Sbjct: 86  LCSNCKIRVHNCCPTCRYDLGNIRCLALEKVAESLELPCKYQNLGCQDIFPYYSKLKHEQ 145

Query: 122 HCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATW 181
           HCRFRPYNCPYAGS+CSVTGDIP+LV+HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATW
Sbjct: 146 HCRFRPYNCPYAGSDCSVTGDIPTLVSHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATW 205

Query: 182 MLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQG 241
           MLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKLIWQG
Sbjct: 206 MLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGANGRKLIWQG 265

Query: 242 IPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           IPR IRDSHRKVRDSQDGL+IQRNLALYFSGG+R+ELKLR+TGRIWKEE
Sbjct: 266 IPRSIRDSHRKVRDSQDGLVIQRNLALYFSGGDRQELKLRVTGRIWKEE 314

BLAST of CmaCh03G001010 vs. NCBI nr
Match: gi|566208943|ref|XP_002323331.2| (hypothetical protein POPTR_0016s05970g [Populus trichocarpa])

HSP 1 Score: 544.3 bits (1401), Expect = 1.3e-151
Identity = 247/282 (87.59%), Postives = 265/282 (93.97%), Query Frame = 1

Query: 2   SKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCTNLMYPPIYQCPNGHTLCSNCKI 61
           SKSENN   +K  +  SGK GVYS NGV ELLECPVCTNLMYPPI+QCPNGHTLCS CK+
Sbjct: 23  SKSENNIAPTKGTVILSGKHGVYSNNGVHELLECPVCTNLMYPPIHQCPNGHTLCSACKL 82

Query: 62  RVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPY 121
           RV NCCPTCR++LGNIRCLALEKVAESLE+PC+YQ+LGC D+FPYYSKLKHEQHCRFRPY
Sbjct: 83  RVHNCCPTCRYDLGNIRCLALEKVAESLELPCKYQSLGCLDVFPYYSKLKHEQHCRFRPY 142

Query: 122 NCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 181
           +CPYAGSECSVTGDIP+L AHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC
Sbjct: 143 SCPYAGSECSVTGDIPALAAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNC 202

Query: 182 FGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRD 241
           FGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKKFSYSLEVG NGRKL+WQGIPR IRD
Sbjct: 203 FGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKKFSYSLEVGGNGRKLVWQGIPRSIRD 262

Query: 242 SHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 284
           SHRKVRDSQDGLIIQRNLALYFSGG+R+ELKLR+TGR+WKEE
Sbjct: 263 SHRKVRDSQDGLIIQRNLALYFSGGDRKELKLRVTGRVWKEE 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SINA2_ARATH2.4e-15087.05E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1[more]
SINA1_ARATH4.3e-14785.51Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE... [more]
SINA3_ARATH2.8e-13076.53E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1[more]
SINA4_ARATH3.2e-12671.99E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1[more]
SINA5_ARATH4.1e-12678.43E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
B9HA10_POPTR1.4e-15287.94E3 ubiquitin-protein ligase OS=Populus trichocarpa GN=POPTR_0006s208602g PE=3 SV... [more]
B9T806_RICCO5.2e-15288.30E3 ubiquitin-protein ligase OS=Ricinus communis GN=RCOM_0446280 PE=3 SV=1[more]
A0A0D2QH79_GOSRA6.8e-15286.16E3 ubiquitin-protein ligase OS=Gossypium raimondii GN=B456_002G223600 PE=3 SV=1[more]
B9IIA7_POPTR8.9e-15287.59E3 ubiquitin-protein ligase OS=Populus trichocarpa GN=POPTR_0016s05970g PE=3 SV=... [more]
A0A067JYP2_JATCU2.0e-15187.94E3 ubiquitin-protein ligase OS=Jatropha curcas GN=JCGZ_25565 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G58040.11.4e-15187.05 seven in absentia of Arabidopsis 2[more]
AT2G41980.12.4e-14885.51 Protein with RING/U-box and TRAF-like domains[more]
AT3G61790.11.6e-13176.53 Protein with RING/U-box and TRAF-like domains[more]
AT4G27880.11.8e-12771.99 Protein with RING/U-box and TRAF-like domains[more]
AT5G53360.17.1e-10077.45 TRAF-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|224088178|ref|XP_002308357.1|2.0e-15287.94Ubiquitin ligase SINAT2 family protein [Populus trichocarpa][more]
gi|743922429|ref|XP_011005289.1|2.6e-15288.38PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Populus euphratica][more]
gi|255587731|ref|XP_002534375.1|7.5e-15288.30PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Ricinus communis][more]
gi|823136405|ref|XP_012467963.1|9.8e-15286.16PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Gossypium raimondii][more]
gi|566208943|ref|XP_002323331.2|1.3e-15187.59hypothetical protein POPTR_0016s05970g [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR004162SINA-like
IPR008974TRAF-like
IPR013010Znf_SIAH
IPR013083Znf_RING/FYVE/PHD
IPR013323SIAH-type domain
IPR0181217-in-absentia-prot_TRAF-dom
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0004842ubiquitin-protein transferase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Biological Process
TermDefinition
GO:0006511ubiquitin-dependent protein catabolic process
GO:0007275multicellular organism development
GO:0016567protein ubiquitination
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh03G001010.1CmaCh03G001010.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 35..71
score: 9
IPR004162E3 ubiquitin-protein ligase SIN-likePANTHERPTHR10315SEVEN IN ABSENTIA HOMOLOGcoord: 23..283
score: 8.7E
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 153..280
score: 7.4
IPR008974TRAF-likeunknownSSF49599TRAF domain-likecoord: 88..280
score: 7.19
IPR013010Zinc finger, SIAH-typePROFILEPS51081ZF_SIAHcoord: 88..148
score: 1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 28..101
score: 4.8
IPR013323SIAH-type domainGENE3DG3DSA:3.90.890.10coord: 102..143
score: 1.8
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 77..276
score: 3.4
NoneNo IPR availablePANTHERPTHR10315:SF34E3 UBIQUITIN-PROTEIN LIGASE SINAT3-RELATEDcoord: 23..283
score: 8.7E
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 29..76
score: 4.0