CmaCh01G003390 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh01G003390 vs. Swiss-Prot
Match: NICA_ARATH (Nicastrin OS=Arabidopsis thaliana GN=At3g52640/At3g52650 PE=2 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.5e-229
Identity = 400/644 (62.11%), Postives = 488/644 (75.78%), Query Frame = 1

Query: 8   SMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIFQPSAI 67
           S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV P+I  KD  ++ QP  I
Sbjct: 33  SIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVAPIIKLKDVKDLVQPHTI 92

Query: 68  LVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKID 127
           LV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFSP ++FPQA+F+PY+ ++
Sbjct: 93  LVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFSPDKRFPQAQFSPYENVE 152

Query: 128 YEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKA 187
           Y+WN   S +MW  YNFPV+L+SES IS V E  SK       YTS+VAEF++VM+TTKA
Sbjct: 153 YKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKA 212

Query: 188 GTHNSMSCLKEETCLPLGGYSVWSSLPPINISLDKS-KPIILTVASMDSASFFRDKSIGA 247
           GTHNS +CL+E TCLPLGGYSVWSSLPPI++S   + KP++LTVASMD+ASFFRDKS GA
Sbjct: 213 GTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGA 272

Query: 248 DSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDSVS 307
           DSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYLGSRRFL ELDL SD+V+
Sbjct: 273 DSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYLGSRRFLHELDLHSDAVA 332

Query: 308 GLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFENIKVS 367
           GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +ALK+AQ+SL  +NIK+ 
Sbjct: 333 GLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLDALKIAQDSLASKNIKIL 392

Query: 368 PASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSAIEAAA 427
            A T NPGIPPSSLMAF+ K                      SHLDDL NINSS++ AAA
Sbjct: 393 SADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHSHLDDLSNINSSSVVAAA 452

Query: 428 LLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYISPINV 487
            +VARTLYILA++ K+ S+SAL +I +N S VEE++ CLL C+PGLSC LVK YISP N 
Sbjct: 453 SVVARTLYILASDNKDTSNSALGSIHVNASFVEELLTCLLACEPGLSCNLVKDYISPTNT 512

Query: 488 CPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQN-CDDKSEVC 547
           CP +Y GVIL EPSS PY GYV DVSRF+WNFLAD+TS+ K NT+SVCS+  C    EVC
Sbjct: 513 CPGNYAGVILGEPSSKPYLGYVGDVSRFLWNFLADKTSVQKGNTTSVCSKGVCSKTDEVC 572

Query: 548 IGAETGK-GTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNWNTIG 607
           I AE+ K GTCVVSTTRYVPAYSTRL +  G+W +LP NSSD MG VDPVWTESNW+T+ 
Sbjct: 573 IKAESNKEGTCVVSTTRYVPAYSTRLKYNDGAWTILPQNSSDSMGMVDPVWTESNWDTLR 632

Query: 608 LRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           +  YTVQ +AYD  VL+ GIT T L+Y  I+A +  I KALK+D
Sbjct: 633 VHVYTVQHSAYDNAVLVAGITVTTLAYIGILAAKSIITKALKQD 676

BLAST of CmaCh01G003390 vs. Swiss-Prot
Match: NICA_DICDI (Nicastrin OS=Dictyostelium discoideum GN=ncstn PE=3 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 4.5e-32
Identity = 98/305 (32.13%), Postives = 162/305 (53.11%), Query Frame = 1

Query: 3   STDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP-GREKVVVPMINFKDADEI 62
           STD  S +S   +++ MY  ++ YPC R++ L+G+IGCS+  G +  ++ +I   D+DE 
Sbjct: 17  STDVISSQS--SIEDKMYTSLNSYPCTRIMTLNGQIGCSSSHGGDSGILYLI---DSDES 76

Query: 63  F-------QPSAILVSMDDISSFFAR-LQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQ 122
           +       Q   I+V  D  S++F + L  +      + G L+   T+I K T  +SP  
Sbjct: 77  YHNYFSYNQQKDIIVVFD--SNYFNKTLVLEMYSKKKMNGALVL--TDIGK-TYPYSPED 136

Query: 123 KFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTS 182
           ++P  +F  Y   +  WNP G G  +  + FP+F +   +   ++  ++ N   K  Y +
Sbjct: 137 QYPIKQFGLYPDSNLNWNPNGDGFTYMNFPFPMFALELKTSIIIRNLSTINRDGK--YPA 196

Query: 183 NVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISLDKSKPIILTVASM 242
             AE D  MQ    G  N+ +CL+   C P+GG S+WSS   +   +D+SKPIIL +  +
Sbjct: 197 YGAELDSFMQ----GAINAETCLRRGFCEPVGGQSIWSSFSEV---IDQSKPIILVMLPI 256

Query: 243 DSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRR 299
           D+ +FFRD + G D     L  LL+ ++ L  VD      K+++F ++  E WGY+GS  
Sbjct: 257 DATAFFRDLATGTDQSGYALTVLLSMLNTLQGVD-KTKWDKEVIFAMWNSERWGYVGSTN 301

BLAST of CmaCh01G003390 vs. Swiss-Prot
Match: NICA_RAT (Nicastrin OS=Rattus norvegicus GN=Ncstn PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 8.5e-23
Identity = 98/391 (25.06%), Postives = 180/391 (46.04%), Query Frame = 1

Query: 27  PCIRLLNLSGEIGC-SNPGREKVVVPMINFKD------ADEIFQPSAILVSMDDIS-SFF 86
           PC+RLLN + +IGC S+   +  V+ ++  +D       D    P  +L+     +    
Sbjct: 48  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEDDLKWVLTDGPNPPYMVLLEGKLFTRDIM 107

Query: 87  ARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKI---------DYE 146
            +L+ +++  + +   L KP +     T  FSP+ + P   F  Y               
Sbjct: 108 EKLKGETSRIAGLAVTLAKPNS-----TSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTL 167

Query: 147 WNPIGSGVMWNQYNFPVFLISESSISPVQE-------------AASKNVKDKKVYTSNVA 206
           WN +G+G+ ++ ++FP+FL+ + + + V +             A S  +   ++++   A
Sbjct: 168 WNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHA 227

Query: 207 EFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINIS--LDKSKPIILTVASMD 266
                    ++   ++ S   E  C PL  Y+VWS L PIN S  L+    +++    +D
Sbjct: 228 VISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLD 287

Query: 267 SASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRF 326
           S SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R 
Sbjct: 288 SRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRM 347

Query: 327 LLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS-KNETWNALKL 385
           + +++     V  L N  ID+  E+G V  +++         M++ + S KN+  + L  
Sbjct: 348 VYDMENGKFPVR-LEN--IDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVT 407

BLAST of CmaCh01G003390 vs. Swiss-Prot
Match: NICA_HUMAN (Nicastrin OS=Homo sapiens GN=NCSTN PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 1.1e-22
Identity = 95/394 (24.11%), Postives = 169/394 (42.89%), Query Frame = 1

Query: 27  PCIRLLNLSGEIGCSNP--GREKVVVPMINFKDADEIFQPS------AILVSMDDISSFF 86
           PC+RLLN + +IGC +   G   V+  +   +D   +           +L S        
Sbjct: 49  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLM 108

Query: 87  ARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKI---------DYE 146
            +L+  ++  + +   L KP         GFSP+ + P   F  Y            + +
Sbjct: 109 EKLKGRTSRIAGLAVSLTKPSP-----ASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQ 168

Query: 147 WNPIGSGVMWNQYNFPVFLISESSISPV--QEAASKNVKDK-----------KVYTSNVA 206
           WN +G+G+ +  ++FP+FL+ + + + V  Q     N+              ++++   A
Sbjct: 169 WNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHA 228

Query: 207 EFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINIS--LDKSKPIILTVASMD 266
                    ++   ++ S   E  C PL  Y+VWS L PIN +  L     +++    +D
Sbjct: 229 VISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLD 288

Query: 267 SASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRF 326
           S SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R 
Sbjct: 289 SRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRM 348

Query: 327 LLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLA 385
           + +++     V  L N  +D+  E+G V  +++      + H   VS       N ++  
Sbjct: 349 VYDMEKGKFPVQ-LEN--VDSFVELGQVALRTS---LELWMHTDPVSQKNESVRNQVEDL 408

BLAST of CmaCh01G003390 vs. Swiss-Prot
Match: NICA_MOUSE (Nicastrin OS=Mus musculus GN=Ncstn PE=1 SV=3)

HSP 1 Score: 105.1 bits (261), Expect = 2.8e-21
Identity = 150/673 (22.29%), Postives = 271/673 (40.27%), Query Frame = 1

Query: 27  PCIRLLNLSGEIGC-SNPGREKVVVPMINFKD------ADEIFQPSAILVSMDDIS-SFF 86
           PC+RLLN + +IGC S+   +  V+ ++  ++       D    P  +L+     +    
Sbjct: 48  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLKWVLTDGPNPPYMVLLEGKLFTRDVM 107

Query: 87  ARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKI---------DYE 146
            +L+  ++  + +   L KP +     T  FSP+ + P   F  Y               
Sbjct: 108 EKLKGTTSRIAGLAVTLAKPNS-----TSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTL 167

Query: 147 WNPIGSGVMWNQYNFPVFLISESSISPVQE-------------AASKNVKDKKVYTSNVA 206
           WN +G+G+ +  ++FP+FL+ + + + V +             A S  +   ++++   A
Sbjct: 168 WNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHA 227

Query: 207 EFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINIS--LDKSKPIILTVASMD 266
                    ++   ++ S   E  C PL  Y+VWS L PIN S  L+    +++    +D
Sbjct: 228 VISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAATRLD 287

Query: 267 SASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRF 326
           S SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R 
Sbjct: 288 SRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIGSSRM 347

Query: 327 LLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS-KNETWNALKL 386
           + +++     V  L N  ID+  E+G V  +++         M++ + S KN+  + L  
Sbjct: 348 VYDMENGKFPVR-LEN--IDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLAT 407

Query: 387 AQESLPFENIKVSPASTTNPGIPPSSLMAFLA---------------------KSHLDDL 446
            ++S       V      +  +PPSSL  FL                      +S  D  
Sbjct: 408 LEKSGAGVPEVVLRRLAQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTA 467

Query: 447 HNIN-------------------SSAIEAAALLVARTLYILATNKKELSSSALNAIKLNT 506
            NIN                   + A+   A ++AR LY LA      SS     I+ + 
Sbjct: 468 ENINVTYPEWQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSSS-----IQADP 527

Query: 507 SLVEEII-GCLLNCDPGLSCELVKRYI-SPINVCP-NHYVGVILDEPSSTPYPGYVHDVS 566
             V  ++ G L+  +      ++K  + S ++  P  HY+ V    P++T Y      V 
Sbjct: 528 QTVTRLLYGFLVRANNSWFQSILKHDLRSYLDDRPLQHYIAV--SSPTNTTYV-----VQ 587

Query: 567 RFVWNFLADRTSIPKENTSSVCSQNCDDKSEVCIGAET------GKGTCVVSTTRYVP-- 609
             + N     T++ +E         C D S+V   ++        +G    + T  +P  
Sbjct: 588 YALANLTGKATNLTRE--------QCQDPSKVPNESKDLYEYSWVQGPWNSNRTERLPQC 647

BLAST of CmaCh01G003390 vs. TrEMBL
Match: A0A061FP76_THECC (Zn-dependent exopeptidases superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_043862 PE=4 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 3.5e-257
Identity = 453/648 (69.91%), Postives = 524/648 (80.86%), Query Frame = 1

Query: 3   STDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIF 62
           S   +SMESVPDLQ SMY+VVDGYPC+RL+NLSGEIGCSNPGR+KVV P++ +KD  E+ 
Sbjct: 21  SDQTNSMESVPDLQKSMYMVVDGYPCVRLVNLSGEIGCSNPGRDKVVAPIVKYKDTKELG 80

Query: 63  QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAP 122
           QPSAIL+SMDD+  FF+R+ +DS+FA NVGGVL++ G EIQ + KGFSPAQKFPQA+FAP
Sbjct: 81  QPSAILLSMDDVQGFFSRVSNDSSFARNVGGVLVESGIEIQNKLKGFSPAQKFPQAEFAP 140

Query: 123 YQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVM 182
           Y    YEWNPIG+G MW  YNFPVFL+SESS S +QE   KN K +K YT+NVAEFDLVM
Sbjct: 141 YHNTSYEWNPIGNGDMWKSYNFPVFLLSESSTSTLQEVTMKNEKTEKAYTTNVAEFDLVM 200

Query: 183 QTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPIN-ISLDKSKPIILTVASMDSASFFRD 242
           QTTK GTH+S SCLKEETCLPLGGYSVWS++PPIN  S ++SKPII+TVASMD+ASFFRD
Sbjct: 201 QTTKVGTHDSESCLKEETCLPLGGYSVWSAVPPINSSSSNQSKPIIITVASMDAASFFRD 260

Query: 243 KSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQ 302
           KS+GADSPISG+I+LLAAVDALS VDGLDDL+KQLVF+VFTGE+WGYLGSRRFLLELD Q
Sbjct: 261 KSLGADSPISGVISLLAAVDALSRVDGLDDLNKQLVFLVFTGEAWGYLGSRRFLLELDQQ 320

Query: 303 SDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFE 362
           SD+V GLN+TL++ V EIGS GK  + G   FFAH TEVSS  NE  +ALKLAQESL  E
Sbjct: 321 SDAVRGLNSTLVELVMEIGSTGKGFSQGNKTFFAH-TEVSSGANEALDALKLAQESLKSE 380

Query: 363 NIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSA 422
            + +S A+++NPGIPPSSLMAFL K                      SHLDD  NINSSA
Sbjct: 381 GVTISTANSSNPGIPPSSLMAFLRKNSSTSGIVLEDFDSIFVNKFYHSHLDDSSNINSSA 440

Query: 423 IEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYI 482
           I AAA LVARTLYILA+N K+L+SSA++ I +N SLVEE+I C+L+C+PGLSCELVK YI
Sbjct: 441 IVAAASLVARTLYILASNNKDLTSSAISTISVNASLVEELISCMLDCNPGLSCELVKSYI 500

Query: 483 SPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDK 542
           S  N CP+HYVGV+L EPSSTPYP  V DVSRF+WNFLADRTSIPK NT SVCS +C   
Sbjct: 501 SSTNTCPSHYVGVVLGEPSSTPYPSQVDDVSRFLWNFLADRTSIPKGNT-SVCSHDCGKN 560

Query: 543 SEVCIGAET-GKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNW 602
             +CI AET GKG CV+STTRYVPAYSTRL F+SG+W VLPPNS+DPMG +DPVWTESNW
Sbjct: 561 GGMCIRAETDGKGVCVISTTRYVPAYSTRLKFDSGTWKVLPPNSTDPMGMLDPVWTESNW 620

Query: 603 NTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           NTIGLR YTVQ  AYDR VLLGG+  T+LSYFAIV  R  I KALK+D
Sbjct: 621 NTIGLRVYTVQDPAYDRLVLLGGVAVTVLSYFAIVLTRAYITKALKQD 666

BLAST of CmaCh01G003390 vs. TrEMBL
Match: A0A0B2PHZ8_GLYSO (Nicastrin OS=Glycine soja GN=glysoja_032210 PE=4 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 2.1e-254
Identity = 439/648 (67.75%), Postives = 514/648 (79.32%), Query Frame = 1

Query: 3   STDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIF 62
           S    SMESVPDLQN+MY  +DGYPC+RL+NLSG IGCSNPGR+KVV P++ F++ D I 
Sbjct: 25  SGQSSSMESVPDLQNTMYASIDGYPCVRLMNLSGTIGCSNPGRDKVVAPIVRFENVDRIA 84

Query: 63  QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAP 122
           +PSA+LVS+D+  + F R+ DDS+FAS VGGVL++P T+ QK+ KGFSP QKFPQA+FA 
Sbjct: 85  EPSAVLVSLDEFPTLFTRISDDSSFASKVGGVLVEPSTDFQKKLKGFSPDQKFPQAQFAL 144

Query: 123 YQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVM 182
           Y    YEWNPIGSG+MW  YNFPVFL++ES    +QE  +KN   KK YTSNVAEFDLVM
Sbjct: 145 YHNTSYEWNPIGSGIMWKSYNFPVFLLTESGSKTLQEFVTKNEDTKKSYTSNVAEFDLVM 204

Query: 183 QTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINI-SLDKSKPIILTVASMDSASFFRD 242
           QT K+GTH+S SCLKEETCLPLGGYSVWSSLPPINI SL +SKPI+LTVASMDSASFFRD
Sbjct: 205 QTVKSGTHDSESCLKEETCLPLGGYSVWSSLPPINISSLQRSKPILLTVASMDSASFFRD 264

Query: 243 KSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQ 302
           KS+GADSPISGLIALLAAVDALSH+DGL DL KQLVF VFTGE+WGYLGSRRFL+ELD+ 
Sbjct: 265 KSLGADSPISGLIALLAAVDALSHLDGLGDLSKQLVFAVFTGEAWGYLGSRRFLVELDMH 324

Query: 303 SDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFE 362
           SD+V GLN TLI+TV EIGSVGK  + G  NFFAH    SS+ N+T  ALK AQESL  E
Sbjct: 325 SDAVHGLNQTLIETVIEIGSVGKGLSQGVKNFFAHTKGDSSATNQTVAALKRAQESLISE 384

Query: 363 NIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSA 422
           NIK++ AS +NPGIPPSSLM+FL K                      SHLDDL N+NSSA
Sbjct: 385 NIKIASASASNPGIPPSSLMSFLEKNPAISGVVLEDFDSVFVNKFYHSHLDDLSNVNSSA 444

Query: 423 IEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYI 482
           + AAA L+ARTLY+LA+  +++ +S L AI +N SLVE+++GCLL+CDPGLSCELVK+YI
Sbjct: 445 VVAAASLIARTLYMLASETEDVQNSTLAAINVNVSLVEQLLGCLLDCDPGLSCELVKKYI 504

Query: 483 SPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDK 542
           SP++ CP+HYVGVILDEPSS PY GY++DV RF+WNFLADRTSIP+EN  S C   C+ +
Sbjct: 505 SPMSTCPSHYVGVILDEPSSAPYAGYINDVPRFIWNFLADRTSIPRENNISDCQHGCNGR 564

Query: 543 SEVCIGAET-GKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNW 602
            EVC+ AET GKG CV+STTRYVPAYSTRL FESG WNVLPPNSSD MG VDPVWTESNW
Sbjct: 565 DEVCVKAETDGKGVCVLSTTRYVPAYSTRLKFESGVWNVLPPNSSDKMGVVDPVWTESNW 624

Query: 603 NTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           NTIG+R YTVQ  AYDR VL GGIT T+ +Y AI   R    KA+K+D
Sbjct: 625 NTIGMRVYTVQNAAYDRLVLFGGITLTVFAYLAIATARAFFNKAMKRD 672

BLAST of CmaCh01G003390 vs. TrEMBL
Match: D7TSK0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0006g00380 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 2.8e-254
Identity = 442/643 (68.74%), Postives = 526/643 (81.80%), Query Frame = 1

Query: 8   SMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIFQPSAI 67
           S+ESVPDL+ SMY+VVDGYPC+RLLNLSGEIGCSNPGREKVV P++ FK+ + + Q SAI
Sbjct: 29  SLESVPDLEKSMYMVVDGYPCVRLLNLSGEIGCSNPGREKVVAPIVRFKNVNVLAQSSAI 88

Query: 68  LVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKID 127
           LVS+D+I SFF RL  DSNFA NVGGVL++  +  Q + KGFSP +KFPQA+FAPYQ I+
Sbjct: 89  LVSLDEIQSFFTRLSHDSNFARNVGGVLVESVSASQNKLKGFSPVEKFPQAEFAPYQSIN 148

Query: 128 YEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKA 187
           YEWNPIGSG+MWN YNFP+FL+S+SS   +QE A KN K+KK YT++VAEFDLVMQTTK+
Sbjct: 149 YEWNPIGSGIMWNTYNFPMFLLSQSSTLTLQEVAIKNEKNKKAYTADVAEFDLVMQTTKS 208

Query: 188 GTHNSMSCLKEETCLPLGGYSVWSSLPPINI-SLDKSKPIILTVASMDSASFFRDKSIGA 247
           GTH+S SCLKEETCLPLGGYSVWSSLPPIN+ S D+SKP+ILTVASMDSASFFRDKS+GA
Sbjct: 209 GTHDSESCLKEETCLPLGGYSVWSSLPPINVSSSDQSKPVILTVASMDSASFFRDKSLGA 268

Query: 248 DSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDSVS 307
           DSPISGLI+L+AAVDALSH+DGL+DL KQLVF+VFTGE+WGYLGSRRFLLELDL SD V 
Sbjct: 269 DSPISGLISLMAAVDALSHLDGLNDLSKQLVFLVFTGEAWGYLGSRRFLLELDLHSDFVK 328

Query: 308 GLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFENIKVS 367
           G+++TLI+ V EIGSVGK  + G   FFAH+ EVSS  NET NAL+ A++SL  E+I +S
Sbjct: 329 GIDSTLIEMVMEIGSVGKGFSQGVKTFFAHVAEVSSVTNETLNALQQAKDSLKSESIMIS 388

Query: 368 PASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSAIEAAA 427
            A+++NPGIPPSSLM+FL K                      SHLDDL N+NSSAI AAA
Sbjct: 389 TANSSNPGIPPSSLMSFLRKNSSTSGIVLEDFDATFANQFYHSHLDDLSNVNSSAIVAAA 448

Query: 428 LLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYISPINV 487
            LVARTLYILA++ K+LS+SAL+AI +N SLVE ++GCLLNCDPGLSC+LVK+YI+P   
Sbjct: 449 SLVARTLYILASDDKDLSTSALSAINVNASLVEALLGCLLNCDPGLSCDLVKKYIAPRTN 508

Query: 488 CPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDKSEVCI 547
           CP++YVGV+L EPS+T YPGYV DVSRF+WNFLADRTSIP+EN +S C ++C ++ EVCI
Sbjct: 509 CPSNYVGVLLGEPSATLYPGYVSDVSRFIWNFLADRTSIPRENATSACPKDCSNEGEVCI 568

Query: 548 GAE-TGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNWNTIGL 607
           G E  GKG CV+STTRYVPAYSTRLMFESG W V+P NSS+ MG  DPVWTESNW+ IGL
Sbjct: 569 GEELDGKGVCVISTTRYVPAYSTRLMFESGIWKVMPLNSSNSMGTEDPVWTESNWDAIGL 628

Query: 608 RTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           R YTVQ  AYDR VLL G+  T+L+Y AIV  R  I KALK+D
Sbjct: 629 RVYTVQNAAYDRLVLLAGLVVTVLAYLAIVVARAFITKALKQD 671

BLAST of CmaCh01G003390 vs. TrEMBL
Match: A0A0L9U8X5_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g262000 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 3.7e-251
Identity = 435/648 (67.13%), Postives = 513/648 (79.17%), Query Frame = 1

Query: 3   STDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIF 62
           S    SMESVPDLQ++MY  ++GYPC+RLLNLSG IGCSNPGR+KVV P++ F + D+I 
Sbjct: 22  SGQSSSMESVPDLQHTMYASIEGYPCVRLLNLSGNIGCSNPGRDKVVAPIVRFGNVDKIA 81

Query: 63  QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAP 122
           +PSA+LVS++D  + F R+ DDS FAS V GVL++P T++Q +  GFSP QKFPQ +FAP
Sbjct: 82  EPSAVLVSVEDFPTLFTRISDDSRFASKVSGVLVEPSTDLQNKINGFSPDQKFPQGQFAP 141

Query: 123 YQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVM 182
           Y    YEWNPIGSGVMW  YNFPVFL++ES    +QE  +KN   KK YTSNVAEFDLVM
Sbjct: 142 YHNTGYEWNPIGSGVMWKSYNFPVFLLTESGSKTLQEFVTKNEDKKKAYTSNVAEFDLVM 201

Query: 183 QTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINI-SLDKSKPIILTVASMDSASFFRD 242
           QT K+GT +S SCLKEETCLPLGGYSVWSSLPPIN  S  +SKPI++TVASMDSASFFRD
Sbjct: 202 QTVKSGTLDSESCLKEETCLPLGGYSVWSSLPPINTSSSQQSKPILMTVASMDSASFFRD 261

Query: 243 KSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQ 302
           K++GADSPISGLIALLAAVDALSH+DGL DL KQLVF VFTGE+WGYLGSRRFL+ELDL 
Sbjct: 262 KTLGADSPISGLIALLAAVDALSHLDGLGDLSKQLVFAVFTGEAWGYLGSRRFLVELDLH 321

Query: 303 SDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFE 362
           SD+V GLN+ LI+ V EIGSVGK  + G  NFFAH    SS+ N+T  ALK AQESL +E
Sbjct: 322 SDAVHGLNHGLIEKVIEIGSVGKGLSGGVNNFFAHTEGDSSATNQTMGALKRAQESLLYE 381

Query: 363 NIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSA 422
           NIK++ AS++NPGIPPSSLM+FL K                      SHLDDL N+NSSA
Sbjct: 382 NIKIASASSSNPGIPPSSLMSFLEKNPAISGVVLEDFDSVFVNKFYHSHLDDLSNVNSSA 441

Query: 423 IEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYI 482
           + AAA LVARTLYILA+  +++  S L+AI +N SLVE+++GCLL+C+PGLSCELVK+YI
Sbjct: 442 VVAAASLVARTLYILASKNEDVHDSTLSAINVNVSLVEQLMGCLLDCNPGLSCELVKKYI 501

Query: 483 SPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDK 542
           SP++ CP+HYVGVILDEPSSTPY GY++DV RF+WNFLADRTSIP+E  SS C   C+D+
Sbjct: 502 SPMSTCPSHYVGVILDEPSSTPYTGYINDVPRFIWNFLADRTSIPRETNSSGCQHGCNDR 561

Query: 543 SEVCIGAET-GKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNW 602
            EVCI AET GKG CV+STTRYVPAYSTRL FESG WNVLPPNSS+ MG VDPVWTESNW
Sbjct: 562 DEVCIKAETDGKGVCVLSTTRYVPAYSTRLKFESGVWNVLPPNSSEKMGVVDPVWTESNW 621

Query: 603 NTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           N+IG+R YTVQ TAYDR VL GGIT TI+SY AI   R    KA+K+D
Sbjct: 622 NSIGMRVYTVQNTAYDRLVLFGGITLTIISYLAIATARTLFSKAMKRD 669

BLAST of CmaCh01G003390 vs. TrEMBL
Match: A0A0S3RZR2_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G369400 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 3.7e-251
Identity = 434/643 (67.50%), Postives = 512/643 (79.63%), Query Frame = 1

Query: 8   SMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIFQPSAI 67
           SMESVPDLQ++MY  ++GYPC+RLLNLSG IGCSNPGR+KVV P++ F + D+I +PSA+
Sbjct: 58  SMESVPDLQHTMYASIEGYPCVRLLNLSGNIGCSNPGRDKVVAPIVRFGNVDKIAEPSAV 117

Query: 68  LVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKID 127
           LVS++D  + F R+ DDS FAS V GVL++P T++Q +  GFSP QKFPQ +FAPY    
Sbjct: 118 LVSVEDFPTLFTRISDDSRFASKVSGVLVEPSTDLQNKINGFSPDQKFPQGQFAPYHNTG 177

Query: 128 YEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKA 187
           YEWNPIGSGVMW  YNFPVFL++ES    +QE  +KN   KK YTSNVAEFDLVMQT K+
Sbjct: 178 YEWNPIGSGVMWKSYNFPVFLLTESGSKTLQEFVTKNEDKKKAYTSNVAEFDLVMQTVKS 237

Query: 188 GTHNSMSCLKEETCLPLGGYSVWSSLPPINI-SLDKSKPIILTVASMDSASFFRDKSIGA 247
           GT +S SCLKEETCLPLGGYSVWSSLPPIN  S  +SKPI++TVASMDSASFFRDK++GA
Sbjct: 238 GTLDSESCLKEETCLPLGGYSVWSSLPPINTSSSQQSKPILMTVASMDSASFFRDKTLGA 297

Query: 248 DSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDSVS 307
           DSPISGLIALLAAVDALSH+DGL DL KQLVF VFTGE+WGYLGSRRFL+ELDL SD+V 
Sbjct: 298 DSPISGLIALLAAVDALSHLDGLGDLSKQLVFAVFTGEAWGYLGSRRFLVELDLHSDAVH 357

Query: 308 GLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFENIKVS 367
           GLN+ LI+ V EIGSVGK  + G  NFFAH    SS+ N+T  ALK AQESL +ENIK++
Sbjct: 358 GLNHGLIEKVIEIGSVGKGLSGGVNNFFAHTEGDSSATNQTMGALKRAQESLLYENIKIA 417

Query: 368 PASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSAIEAAA 427
            AS++NPGIPPSSLM+FL K                      SHLDDL N+NSSA+ AAA
Sbjct: 418 SASSSNPGIPPSSLMSFLEKNPAISGVVLEDFDSVFVNKFYHSHLDDLSNVNSSAVVAAA 477

Query: 428 LLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYISPINV 487
            LVARTLYILA+  +++  S L+AI +N SLVE+++GCLL+C+PGLSCELVK+YISP++ 
Sbjct: 478 SLVARTLYILASKNEDVHDSTLSAINVNVSLVEQLMGCLLDCNPGLSCELVKKYISPMST 537

Query: 488 CPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDKSEVCI 547
           CP+HYVGVILDEPSSTPY GY++DV RF+WNFLADRTSIP+E  SS C   C+D+ EVCI
Sbjct: 538 CPSHYVGVILDEPSSTPYTGYINDVPRFIWNFLADRTSIPRETNSSGCQHGCNDRDEVCI 597

Query: 548 GAET-GKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNWNTIGL 607
            AET GKG CV+STTRYVPAYSTRL FESG WNVLPPNSS+ MG VDPVWTESNWN+IG+
Sbjct: 598 KAETDGKGVCVLSTTRYVPAYSTRLKFESGVWNVLPPNSSEKMGVVDPVWTESNWNSIGM 657

Query: 608 RTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           R YTVQ TAYDR VL GGIT TI+SY AI   R    KA+K+D
Sbjct: 658 RVYTVQNTAYDRLVLFGGITLTIISYLAIATARTLFSKAMKRD 700

BLAST of CmaCh01G003390 vs. TAIR10
Match: AT3G52640.2 (AT3G52640.2 Zn-dependent exopeptidases superfamily protein)

HSP 1 Score: 781.2 bits (2016), Expect = 4.9e-226
Identity = 400/673 (59.44%), Postives = 488/673 (72.51%), Query Frame = 1

Query: 8   SMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIFQPSAI 67
           S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV P+I  KD  ++ QP  I
Sbjct: 33  SIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVAPIIKLKDVKDLVQPHTI 92

Query: 68  LVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKID 127
           LV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFSP ++FPQA+F+PY+ ++
Sbjct: 93  LVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFSPDKRFPQAQFSPYENVE 152

Query: 128 YEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKA 187
           Y+WN   S +MW  YNFPV+L+SES IS V E  SK       YTS+VAEF++VM+TTKA
Sbjct: 153 YKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKA 212

Query: 188 GTHNSMSCLKEETCLPLGGYSVWSSLPPINISLDKS-KPIILTVASMDSASFFRDKSIGA 247
           GTHNS +CL+E TCLPLGGYSVWSSLPPI++S   + KP++LTVASMD+ASFFRDKS GA
Sbjct: 213 GTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGA 272

Query: 248 DSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSDSVS 307
           DSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYLGSRRFL ELDL SD+V+
Sbjct: 273 DSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYLGSRRFLHELDLHSDAVA 332

Query: 308 GLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFENIKVS 367
           GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +ALK+AQ+SL  +NIK+ 
Sbjct: 333 GLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLDALKIAQDSLASKNIKIL 392

Query: 368 PASTTNPGIPPSSLMAFLAK----------------------SHLDDL------------ 427
            A T NPGIPPSSLMAF+ K                      SHLDDL            
Sbjct: 393 SADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHSHLDDLCKKSHSLSFSSF 452

Query: 428 -----------------HNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSL 487
                             NINSS++ AAA +VARTLYILA++ K+ S+SAL +I +N S 
Sbjct: 453 RSKPHFALLIPFWCCIAANINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASF 512

Query: 488 VEEIIGCLLNCDPGLSCELVKRYISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWN 547
           VEE++ CLL C+PGLSC LVK YISP N CP +Y GVIL EPSS PY GYV DVSRF+WN
Sbjct: 513 VEELLTCLLACEPGLSCNLVKDYISPTNTCPGNYAGVILGEPSSKPYLGYVGDVSRFLWN 572

Query: 548 FLADRTSIPKENTSSVCSQN-CDDKSEVCIGAETGK-GTCVVSTTRYVPAYSTRLMFESG 607
           FLAD+TS+ K NT+SVCS+  C    EVCI AE+ K GTCVVSTTRYVPAYSTRL +  G
Sbjct: 573 FLADKTSVQKGNTTSVCSKGVCSKTDEVCIKAESNKEGTCVVSTTRYVPAYSTRLKYNDG 632

Query: 608 SWNVLPPNSSDPMGAVDPVWTESNWNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIV 627
           +W +LP NSSD MG VDPVWTESNW+T+ +  YTVQ +AYD  VL+ GIT T L+Y  I+
Sbjct: 633 AWTILPQNSSDSMGMVDPVWTESNWDTLRVHVYTVQHSAYDNAVLVAGITVTTLAYIGIL 692

BLAST of CmaCh01G003390 vs. NCBI nr
Match: gi|659114563|ref|XP_008457115.1| (PREDICTED: nicastrin isoform X1 [Cucumis melo])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 559/649 (86.13%), Postives = 591/649 (91.06%), Query Frame = 1

Query: 1   MHSTDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADE 60
           + S+DE  MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNPGREKVV+PMINFKDADE
Sbjct: 35  LSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPGREKVVLPMINFKDADE 94

Query: 61  IFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKF 120
           I +PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ RT+GFSPAQKFPQAKF
Sbjct: 95  ILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKF 154

Query: 121 APYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDL 180
           APY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKNVK KK Y SNVAEFDL
Sbjct: 155 APYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFDL 214

Query: 181 VMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISL-DKSKPIILTVASMDSASFF 240
           VMQTTKAGTH+SMSCLKEETCLPLGGYSVWSSLPPIN S  D+SKP+ILTVASMDSASFF
Sbjct: 215 VMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFF 274

Query: 241 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELD 300
           RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELD
Sbjct: 275 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELD 334

Query: 301 LQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLP 360
           LQSD+VSGL+N LID VFEIGSVGK SN G GNFFAHMTEVSSSKNETWNALKLA+ESLP
Sbjct: 335 LQSDAVSGLSNRLIDMVFEIGSVGKSSNHGSGNFFAHMTEVSSSKNETWNALKLARESLP 394

Query: 361 FENIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINS 420
            ENIKVSPASTTNPGIPPSSLMAFLAK                      SHLDDLHNINS
Sbjct: 395 LENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINS 454

Query: 421 SAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKR 480
           SAIEAAALLVARTLYILA NK ELSSS L AIK+NTSLVEE+IGCLLNCDPGLSCELVKR
Sbjct: 455 SAIEAAALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKR 514

Query: 481 YISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD 540
           YI+P +VCPNHYVGVILDEPSS PYP YVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD
Sbjct: 515 YITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD 574

Query: 541 DKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESN 600
           D+SEVCIGAETGKGTCV+STTRY+PAYSTRL FESG W+VLPPNSSD +GAVDPVWTESN
Sbjct: 575 DRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESN 634

Query: 601 WNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           WNTIGLR YT+QA AYDRFVLLGGITTTIL+YFAIVAVR SI+KALK+D
Sbjct: 635 WNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIKALKRD 683

BLAST of CmaCh01G003390 vs. NCBI nr
Match: gi|449445945|ref|XP_004140732.1| (PREDICTED: nicastrin [Cucumis sativus])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 560/649 (86.29%), Postives = 587/649 (90.45%), Query Frame = 1

Query: 1   MHSTDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADE 60
           + S+DEHSMESVPDLQNSMYL VD YPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADE
Sbjct: 17  LSSSDEHSMESVPDLQNSMYLAVDAYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADE 76

Query: 61  IFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKF 120
           I QPSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ RT+GFSPAQKFPQAKF
Sbjct: 77  ILQPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKF 136

Query: 121 APYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDL 180
           APY+K DYEWNP GSG+MWNQYNFPVFLISESSIS +QEAASKNVK KK Y SNVAEFDL
Sbjct: 137 APYEKSDYEWNPSGSGIMWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDL 196

Query: 181 VMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISL-DKSKPIILTVASMDSASFF 240
           VMQTTKAGTH+SMSCLKEETCLPLGGYSVWSSLPPIN S  D+SKP+ILTVASMDSASFF
Sbjct: 197 VMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFF 256

Query: 241 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELD 300
           RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVF VFTGESWGYLGSRRFLLELD
Sbjct: 257 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFAVFTGESWGYLGSRRFLLELD 316

Query: 301 LQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLP 360
           LQSD+VSGL N LIDTVFEIGSVGK S  G GNFFAHMTEVSSS NETWNALKLA+ESLP
Sbjct: 317 LQSDAVSGLENRLIDTVFEIGSVGKSSKHGSGNFFAHMTEVSSSNNETWNALKLARESLP 376

Query: 361 FENIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINS 420
            ENIKVSPASTTNPGIPPSSLMAFLAK                      S+LDDLHNINS
Sbjct: 377 LENIKVSPASTTNPGIPPSSLMAFLAKNPQVSGVVLEDFDTGFTNQFYQSYLDDLHNINS 436

Query: 421 SAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKR 480
           SAIEAAALLVARTLYILA NKKELSSS L AIK+NTSLVEE+IGCLLNCDPGLSCELVKR
Sbjct: 437 SAIEAAALLVARTLYILAINKKELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKR 496

Query: 481 YISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD 540
           YISP +VCPNHYVGVILDEPSS PYP YVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD
Sbjct: 497 YISPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD 556

Query: 541 DKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESN 600
           DKSEVCIGAETGKGTC +STTRY+PAYSTRL FESG W+VLPPNSSD +G VDPVWTESN
Sbjct: 557 DKSEVCIGAETGKGTCAISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGTVDPVWTESN 616

Query: 601 WNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           WNTIGLR YT+QA AYDRFVLLGGITTTIL+YFAIVAVR SI+KALK+D
Sbjct: 617 WNTIGLRVYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIKALKRD 665

BLAST of CmaCh01G003390 vs. NCBI nr
Match: gi|659114565|ref|XP_008457117.1| (PREDICTED: nicastrin isoform X2 [Cucumis melo])

HSP 1 Score: 1055.0 bits (2727), Expect = 4.9e-305
Identity = 538/649 (82.90%), Postives = 570/649 (87.83%), Query Frame = 1

Query: 1   MHSTDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADE 60
           + S+DE  MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNPGREKVV+PMINFKDADE
Sbjct: 35  LSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPGREKVVLPMINFKDADE 94

Query: 61  IFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKF 120
           I +PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ RT+GFSPAQKFPQAKF
Sbjct: 95  ILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQNRTEGFSPAQKFPQAKF 154

Query: 121 APYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDL 180
           APY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKNVK KK Y SNVAEFDL
Sbjct: 155 APYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFDL 214

Query: 181 VMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISL-DKSKPIILTVASMDSASFF 240
           VMQTTKAGTH+SMSCLKEETCLPLGGYSVWSSLPPIN S  D+SKP+ILTVASMDSASFF
Sbjct: 215 VMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFF 274

Query: 241 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELD 300
           RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELD
Sbjct: 275 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELD 334

Query: 301 LQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLP 360
           LQSD+VSGL+N LID V                        SSSKNETWNALKLA+ESLP
Sbjct: 335 LQSDAVSGLSNRLIDMV------------------------SSSKNETWNALKLARESLP 394

Query: 361 FENIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINS 420
            ENIKVSPASTTNPGIPPSSLMAFLAK                      SHLDDLHNINS
Sbjct: 395 LENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVVLDDFDTGFTNQFYQSHLDDLHNINS 454

Query: 421 SAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKR 480
           SAIEAAALLVARTLYILA NK ELSSS L AIK+NTSLVEE+IGCLLNCDPGLSCELVKR
Sbjct: 455 SAIEAAALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIGCLLNCDPGLSCELVKR 514

Query: 481 YISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD 540
           YI+P +VCPNHYVGVILDEPSS PYP YVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD
Sbjct: 515 YITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCD 574

Query: 541 DKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESN 600
           D+SEVCIGAETGKGTCV+STTRY+PAYSTRL FESG W+VLPPNSSD +GAVDPVWTESN
Sbjct: 575 DRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFESGYWSVLPPNSSDHLGAVDPVWTESN 634

Query: 601 WNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           WNTIGLR YT+QA AYDRFVLLGGITTTIL+YFAIVAVR SI+KALK+D
Sbjct: 635 WNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIKALKRD 659

BLAST of CmaCh01G003390 vs. NCBI nr
Match: gi|590566684|ref|XP_007010305.1| (Zn-dependent exopeptidases superfamily protein isoform 1 [Theobroma cacao])

HSP 1 Score: 895.6 bits (2313), Expect = 5.0e-257
Identity = 453/648 (69.91%), Postives = 524/648 (80.86%), Query Frame = 1

Query: 3   STDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIF 62
           S   +SMESVPDLQ SMY+VVDGYPC+RL+NLSGEIGCSNPGR+KVV P++ +KD  E+ 
Sbjct: 21  SDQTNSMESVPDLQKSMYMVVDGYPCVRLVNLSGEIGCSNPGRDKVVAPIVKYKDTKELG 80

Query: 63  QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAP 122
           QPSAIL+SMDD+  FF+R+ +DS+FA NVGGVL++ G EIQ + KGFSPAQKFPQA+FAP
Sbjct: 81  QPSAILLSMDDVQGFFSRVSNDSSFARNVGGVLVESGIEIQNKLKGFSPAQKFPQAEFAP 140

Query: 123 YQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVM 182
           Y    YEWNPIG+G MW  YNFPVFL+SESS S +QE   KN K +K YT+NVAEFDLVM
Sbjct: 141 YHNTSYEWNPIGNGDMWKSYNFPVFLLSESSTSTLQEVTMKNEKTEKAYTTNVAEFDLVM 200

Query: 183 QTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPIN-ISLDKSKPIILTVASMDSASFFRD 242
           QTTK GTH+S SCLKEETCLPLGGYSVWS++PPIN  S ++SKPII+TVASMD+ASFFRD
Sbjct: 201 QTTKVGTHDSESCLKEETCLPLGGYSVWSAVPPINSSSSNQSKPIIITVASMDAASFFRD 260

Query: 243 KSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQ 302
           KS+GADSPISG+I+LLAAVDALS VDGLDDL+KQLVF+VFTGE+WGYLGSRRFLLELD Q
Sbjct: 261 KSLGADSPISGVISLLAAVDALSRVDGLDDLNKQLVFLVFTGEAWGYLGSRRFLLELDQQ 320

Query: 303 SDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFE 362
           SD+V GLN+TL++ V EIGS GK  + G   FFAH TEVSS  NE  +ALKLAQESL  E
Sbjct: 321 SDAVRGLNSTLVELVMEIGSTGKGFSQGNKTFFAH-TEVSSGANEALDALKLAQESLKSE 380

Query: 363 NIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSA 422
            + +S A+++NPGIPPSSLMAFL K                      SHLDD  NINSSA
Sbjct: 381 GVTISTANSSNPGIPPSSLMAFLRKNSSTSGIVLEDFDSIFVNKFYHSHLDDSSNINSSA 440

Query: 423 IEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYI 482
           I AAA LVARTLYILA+N K+L+SSA++ I +N SLVEE+I C+L+C+PGLSCELVK YI
Sbjct: 441 IVAAASLVARTLYILASNNKDLTSSAISTISVNASLVEELISCMLDCNPGLSCELVKSYI 500

Query: 483 SPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDK 542
           S  N CP+HYVGV+L EPSSTPYP  V DVSRF+WNFLADRTSIPK NT SVCS +C   
Sbjct: 501 SSTNTCPSHYVGVVLGEPSSTPYPSQVDDVSRFLWNFLADRTSIPKGNT-SVCSHDCGKN 560

Query: 543 SEVCIGAET-GKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNW 602
             +CI AET GKG CV+STTRYVPAYSTRL F+SG+W VLPPNS+DPMG +DPVWTESNW
Sbjct: 561 GGMCIRAETDGKGVCVISTTRYVPAYSTRLKFDSGTWKVLPPNSTDPMGMLDPVWTESNW 620

Query: 603 NTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           NTIGLR YTVQ  AYDR VLLGG+  T+LSYFAIV  R  I KALK+D
Sbjct: 621 NTIGLRVYTVQDPAYDRLVLLGGVAVTVLSYFAIVLTRAYITKALKQD 666

BLAST of CmaCh01G003390 vs. NCBI nr
Match: gi|734338420|gb|KHN08795.1| (Nicastrin [Glycine soja])

HSP 1 Score: 886.3 bits (2289), Expect = 3.0e-254
Identity = 439/648 (67.75%), Postives = 514/648 (79.32%), Query Frame = 1

Query: 3   STDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEIF 62
           S    SMESVPDLQN+MY  +DGYPC+RL+NLSG IGCSNPGR+KVV P++ F++ D I 
Sbjct: 25  SGQSSSMESVPDLQNTMYASIDGYPCVRLMNLSGTIGCSNPGRDKVVAPIVRFENVDRIA 84

Query: 63  QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAP 122
           +PSA+LVS+D+  + F R+ DDS+FAS VGGVL++P T+ QK+ KGFSP QKFPQA+FA 
Sbjct: 85  EPSAVLVSLDEFPTLFTRISDDSSFASKVGGVLVEPSTDFQKKLKGFSPDQKFPQAQFAL 144

Query: 123 YQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVM 182
           Y    YEWNPIGSG+MW  YNFPVFL++ES    +QE  +KN   KK YTSNVAEFDLVM
Sbjct: 145 YHNTSYEWNPIGSGIMWKSYNFPVFLLTESGSKTLQEFVTKNEDTKKSYTSNVAEFDLVM 204

Query: 183 QTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINI-SLDKSKPIILTVASMDSASFFRD 242
           QT K+GTH+S SCLKEETCLPLGGYSVWSSLPPINI SL +SKPI+LTVASMDSASFFRD
Sbjct: 205 QTVKSGTHDSESCLKEETCLPLGGYSVWSSLPPINISSLQRSKPILLTVASMDSASFFRD 264

Query: 243 KSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQ 302
           KS+GADSPISGLIALLAAVDALSH+DGL DL KQLVF VFTGE+WGYLGSRRFL+ELD+ 
Sbjct: 265 KSLGADSPISGLIALLAAVDALSHLDGLGDLSKQLVFAVFTGEAWGYLGSRRFLVELDMH 324

Query: 303 SDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLAQESLPFE 362
           SD+V GLN TLI+TV EIGSVGK  + G  NFFAH    SS+ N+T  ALK AQESL  E
Sbjct: 325 SDAVHGLNQTLIETVIEIGSVGKGLSQGVKNFFAHTKGDSSATNQTVAALKRAQESLISE 384

Query: 363 NIKVSPASTTNPGIPPSSLMAFLAK----------------------SHLDDLHNINSSA 422
           NIK++ AS +NPGIPPSSLM+FL K                      SHLDDL N+NSSA
Sbjct: 385 NIKIASASASNPGIPPSSLMSFLEKNPAISGVVLEDFDSVFVNKFYHSHLDDLSNVNSSA 444

Query: 423 IEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGCLLNCDPGLSCELVKRYI 482
           + AAA L+ARTLY+LA+  +++ +S L AI +N SLVE+++GCLL+CDPGLSCELVK+YI
Sbjct: 445 VVAAASLIARTLYMLASETEDVQNSTLAAINVNVSLVEQLLGCLLDCDPGLSCELVKKYI 504

Query: 483 SPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTSIPKENTSSVCSQNCDDK 542
           SP++ CP+HYVGVILDEPSS PY GY++DV RF+WNFLADRTSIP+EN  S C   C+ +
Sbjct: 505 SPMSTCPSHYVGVILDEPSSAPYAGYINDVPRFIWNFLADRTSIPRENNISDCQHGCNGR 564

Query: 543 SEVCIGAET-GKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNW 602
            EVC+ AET GKG CV+STTRYVPAYSTRL FESG WNVLPPNSSD MG VDPVWTESNW
Sbjct: 565 DEVCVKAETDGKGVCVLSTTRYVPAYSTRLKFESGVWNVLPPNSSDKMGVVDPVWTESNW 624

Query: 603 NTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD 627
           NTIG+R YTVQ  AYDR VL GGIT T+ +Y AI   R    KA+K+D
Sbjct: 625 NTIGMRVYTVQNAAYDRLVLFGGITLTVFAYLAIATARAFFNKAMKRD 672