Cla023353 (gene) Watermelon (97103) v1

Overview
NameCla023353
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionE3 SUMO-protein ligase NSE2 (AHRD V1 ***- C3KJP6_ANOFI); contains Interpro domain(s) IPR004181 Zinc finger, MIZ-type
LocationChr11 : 19918254 .. 19920632 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCCGCTTCCGATTCCCGTTCAAGCGGCGTTTCCGGTAGAATCAAATCCGCCGCCACTATTATGCACTCCGAGAATCAATCCCTCCTTGCCGTAACTTGCTCAATACTACTCTCTTACTTCTATCTCTCCTTTCAATTTCTTCTTCTGATTGTTCGAGAAAATGAAGTTGCTGTTGCTAAATAAACGTTGTTTTTAGCTATAACATCTTGATTTCCTATTATTGAGTGTCAGTTCTAATGCTGTATTCCCTTCATTTTGCGCTTTGAACTGGGGTTTTCTAGGTTTCTGAAAGCTGAAACGTCAGGGCCGAGATTATCCTATTGAAAAGAATGATTCATAAGGAATTTGACTAATGATATGATGTATCTGTTAATTTTTATGATTGGATTTTATTGTCTTAAATTCAGGAATTGCGGAAGGCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAAAGGGATAACCAATCCAGGATGGTATTGTGAATTATGGACCTTTATTTGGAGCATCATAAATGATTTTCAATTGTTACTTTGGAAAAGAATGTTCTTGGGTTAGGATAAGATCTTGTAATAAAACACTAAAAAGCAAAGTCAACATAAATTTCAAAGATGTTCTATAGAACTCTTTGCATAAGGCTTTATTTCTACAGCTATGGAGTTTATTTCTTAGTTTATTTCAGTAGGTGTACATTTAGTTTAACCTATACCACATGCACTTTTTGGTGGATTTTCTGCTTCTGTCAAGATCCAATCTAAAGATAATTGTGAATCACTGTTGTTTGGAACTCTACCAATAATTTACTTGGTATTTGAACATTAACTTAGGTCAAGGAGCTTGAAAATTCTGTTGTCGAGCTGTTGAGTACTTATGAAGACTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAATATGTATGAACCAAAAGAAGAGGTGCTTAAATCTCTTTCCATTTTCCACAACACATTCTTTTGTTAATTTTCATTGTGTAGGTTCCTTCCTTTTCAGACGTAATATTTTATGTTGATATTTTAGTTAACAGATTTTGGGAAACTACTTGACGATGAAGTTGCAAAACTCAGTGAAAATTCCACTTCAAATTTGCTGAACCATTCAATAATTCGGCAATTTAGAGAAGCTATTTGGGTATGCATCTTCATCTCCTTTTCTATGGTCTGGGAAGGGCCTTGGTACCCATTAGAAAATAGATCGGAAAGCCAGGGGAAAATATTTCCCTTCCCTTTTATATCAGATCTGATTATAGTAAGTAAACCGTTTATTGCGTTATCTCACTTTTGTGACTTTCTATCAATTGGGCTACAGCACTGGTTATGCATGGCAGCAGCAAAAAAATCCTCAAATCTGTGCAAATAATTATCCTAAGACTGTGAAAATGCTCAAAGAAAGTACATAAGTTGTAAACAAGAGAAACTATTTTACCTGTTTGCAATTTACAAATGTTTGGAAAGCATTTTATTACTCTTCAATTTTAGATTTAATTCTTTCCAGTGCTTTAGCCGATCTCAGTATTCCTGCAGAATGTTCATCATTCAGGACAACCAATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATATCACTTGCCCGTTAAGTGGAAAGCCTGTCACCGAATTAGCAGAACCCATTCGCAGGTATAGTGCAACTAAATATATTTTGATGAATAATAAAGAACTGTTATCATTTCCGTTACTATTGAAAAAAATGTCTGCCCATCCATTCGCTGTGTAGTGTGTACTGACAGCATGGATGTTTAAAGGGAATGGCCACATGCTTGTGCCATTTTCTCTGGTTACATTTTTCAAGTGGGTGCCTGAAAATGGAATGAACTAGTCTGTCGCTTTGATCTTCTTCTTCCTCTTCTTCTTATGTTTGAGAGCTGAGTCTTGAAATTGTATTTTTGTTCTTCAAACTATTAATCGTTTTAGGTTGTGGAGAAGGTTCATTCAAATGGATTAGGCCATCGTGTATTTAAACACTTCTATTAAGTCTCTTTTTGTCTCTACCTTCTGGAATTTGTAGTATGGAATGCAAGCACATATATGAAAAGGCAGCCATAATGCAGTATCTTAAATCCAAGAAATCTCGCGCGCAATGCCCGGTTGCAGGTATTCAGATCTACTACATTACATATACTACATACAGAATGTTTACATTGGTTCGTCTCACTTGACCTCTCTGTCCTTGTAGCCTGTCCTAAGATGTTGCAGCCCGAAAAGGTTGTAGCTGATCCGTTCTTACAGATTGAAATTGATGAACTCCGAAAAATGTCTAGGCATTCTGCGAGGATACAAGACTTCACAGAGCTTGATGCAGATTAG

mRNA sequence

ATGGCATCCGCTTCCGATTCCCGTTCAAGCGGCGTTTCCGGTAGAATCAAATCCGCCGCCACTATTATGCACTCCGAGAATCAATCCCTCCTTGCCGAATTGCGGAAGGCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAAAGGGATAACCAATCCAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTCGAGCTGTTGAGTACTTATGAAGACTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAATATGTATGAACCAAAAGAAGAGTTAACAGATTTTGGGAAACTACTTGACGATGAAGTTGCAAAACTCAGTGAAAATTCCACTTCAAATTTGCTGAACCATTCAATAATTCGGCAATTTAGAGAAGCTATTTGGAATGTTCATCATTCAGGACAACCAATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATATCACTTGCCCGTTAAGTGGAAAGCCTGTCACCGAATTAGCAGAACCCATTCGCAGTATGGAATGCAAGCACATATATGAAAAGGCAGCCATAATGCAGTATCTTAAATCCAAGAAATCTCGCGCGCAATGCCCGGTTGCAGCCTGTCCTAAGATGTTGCAGCCCGAAAAGGTTGTAGCTGATCCGTTCTTACAGATTGAAATTGATGAACTCCGAAAAATGTCTAGGCATTCTGCGAGGATACAAGACTTCACAGAGCTTGATGCAGATTAG

Coding sequence (CDS)

ATGGCATCCGCTTCCGATTCCCGTTCAAGCGGCGTTTCCGGTAGAATCAAATCCGCCGCCACTATTATGCACTCCGAGAATCAATCCCTCCTTGCCGAATTGCGGAAGGCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAAAGGGATAACCAATCCAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTCGAGCTGTTGAGTACTTATGAAGACTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAATATGTATGAACCAAAAGAAGAGTTAACAGATTTTGGGAAACTACTTGACGATGAAGTTGCAAAACTCAGTGAAAATTCCACTTCAAATTTGCTGAACCATTCAATAATTCGGCAATTTAGAGAAGCTATTTGGAATGTTCATCATTCAGGACAACCAATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATATCACTTGCCCGTTAAGTGGAAAGCCTGTCACCGAATTAGCAGAACCCATTCGCAGTATGGAATGCAAGCACATATATGAAAAGGCAGCCATAATGCAGTATCTTAAATCCAAGAAATCTCGCGCGCAATGCCCGGTTGCAGCCTGTCCTAAGATGTTGCAGCCCGAAAAGGTTGTAGCTGATCCGTTCTTACAGATTGAAATTGATGAACTCCGAAAAATGTCTAGGCATTCTGCGAGGATACAAGACTTCACAGAGCTTGATGCAGATTAG

Protein sequence

MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKELENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHSIIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMECKHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSARIQDFTELDAD
Homology
[Close]
BLAST of Cla023353 vs. Swiss-Prot
Match: NSE2_ARATH (E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana GN=MMS21 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-76
Identity = 140/244 (57.38%), Postives = 184/244 (75.41%), Query Frame = 1 

Query: 4   ASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKELENS 63
           AS S S GV+GRI++A+ ++ S+N S LA++RKA+ MMK I V LE++NQ+  VK+LENS
Sbjct: 2   ASASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENS 61

Query: 64  VVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHSIIR 123
           V ELL  + DCN+ S+AIQSV N Y+P E+LTDF KLLDDE  KL    +S   N  ++R
Sbjct: 62  VAELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMR 121

Query: 124 QFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMECKHI 183
           QFREA+WNVHH+G+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTELA+P+RSM+C+H+
Sbjct: 122 QFREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHV 181

Query: 184 YEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSAR---IQ 243
           YEK+ I+ Y+ +  + A CPVA C   LQ  KV+ D  L+ EI+E+R +++ S R   I+
Sbjct: 182 YEKSVILHYIVNNPN-ANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIE 241

Query: 244 DFTE 245
           DFTE
Sbjct: 242 DFTE 244

[Close]
BLAST of Cla023353 vs. Swiss-Prot
Match: NSE2_HUMAN (E3 SUMO-protein ligase NSE2 OS=Homo sapiens GN=NSMCE2 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 3.6e-09
Identity = 62/248 (25.00%), Postives = 120/248 (48.39%), Query Frame = 1 

Query: 7   SRSSGVSGRIK-SAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKE--LENS 66
           S +SG +G I  S      S  ++  A +   +     + +DL  ++Q+ +  E  ++ +
Sbjct: 6   SSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLV-ESQTEVSSEYSMDKA 65

Query: 67  VVELLSTYEDCNNFSSAIQSVGNMY--EPKEELTDFGKLLDDEVAKL-SENSTSNLLNHS 126
           +VE  +     N++  A+QS  N    E  E++ D   L++ +   L S+NS ++  N+ 
Sbjct: 66  MVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNE 125

Query: 127 IIRQFREAIWNVH-----HSGQPMPGEE--QEDIVMTSTQCNLLNITCPLSGKPVTELAE 186
              QF++ +  +       + +   G E   EDI++T +Q N    TCP++ +   E+ +
Sbjct: 126 KFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNF---TCPITKE---EMKK 185

Query: 187 PIRSMECKHIYEKAAIMQYLKS---KKSRAQCPVAACPKM-LQPEKVVADPFLQIEID-E 237
           P+++  C H YE+ AI++ ++S   +K +A CP   C    ++   ++ D  L+  I+  
Sbjct: 186 PVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENH 245

[Close]
BLAST of Cla023353 vs. Swiss-Prot
Match: NSE2_BOVIN (E3 SUMO-protein ligase NSE2 OS=Bos taurus GN=NSMCE2 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-08
Identity = 57/249 (22.89%), Postives = 114/249 (45.78%), Query Frame = 1 

Query: 4   ASDSRSSGVSGRIK-SAATIMHSENQSLLAELRKALIMMKEIGVDL-ERDNQSRMVKELE 63
           ++ S SSG +G I  S      S  ++  + +   +     + +DL E   +      ++
Sbjct: 5   STSSSSSGSTGFISFSGVESALSSLKTFQSCISSGMDTASSVALDLVETQTEVSSEYSMD 64

Query: 64  NSVVELLSTYEDCNNFSSAIQSVGNMY--EPKEELTDFGKLLDDEVAKL-SENSTSNLLN 123
            ++VE      + N++  A+QS  N    E  E++ D   L++ +   L ++NS ++  N
Sbjct: 65  KAMVEFAMMDRELNHYLKAVQSTINHVKEERSEKIPDLKLLVEKKFLALQNKNSDADFQN 124

Query: 124 HSIIRQFREAIWNVHHSGQPMPGEE-------QEDIVMTSTQCNLLNITCPLSGKPVTEL 183
           +    QF++ +  +          E        ED+++T +Q N +   CP++     E+
Sbjct: 125 NEKFVQFKQQLKELKKQYGLQSDREADITEGVDEDMIVTQSQTNFI---CPITQ---LEM 184

Query: 184 AEPIRSMECKHIYEKAAIMQYLKSK---KSRAQCPVAACPKM-LQPEKVVADPFLQIEID 237
            +P+++  C H YE+ AI++ ++SK   K +A CP   C  + ++   ++ D  L+  I+
Sbjct: 185 KKPVKNKVCGHTYEEEAIVRMIESKHERKKKACCPKIGCSHVDMRMSDLIQDEALRRAIE 244

[Close]
BLAST of Cla023353 vs. Swiss-Prot
Match: NSE2_RAT (E3 SUMO-protein ligase NSE2 OS=Rattus norvegicus GN=Nsmce2 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 9.0e-08
Identity = 62/252 (24.60%), Postives = 116/252 (46.03%), Query Frame = 1 

Query: 8   RSSGVSGRIKSAATIMHSENQSLLAELR-------KALIMMKEIGVDL-ERDNQSRMVKE 67
           RSS  SG   S   I  S  +S L+ L+         +  +  + +DL E   +      
Sbjct: 4   RSSTNSG---STRYISFSGVESALSSLKTFQSCISSGMDTVSSVALDLVETQTEVSSEYS 63

Query: 68  LENSVVELLSTYEDCNNFSSAIQSVGNMY--EPKEELTDFGKLLDDEVAKLSE-NSTSNL 127
           ++ ++VE      + N++  A+QS  N    E  E++ D   L++ +   L + NS ++ 
Sbjct: 64  MDKAMVEFAKMDRELNHYVKAVQSTINHVKEERPEKVPDLKLLVEKKFLALQDKNSDADF 123

Query: 128 LNHSIIRQFREAIWNVH-----HSGQPMPGEE--QEDIVMTSTQCNLLNITCPLSGKPVT 187
             +    QF++ +  +      H+ +   G E   ED+++T +Q N +   CP++     
Sbjct: 124 KENEKFVQFKQQLRELKKQYGIHADRENDGIEGMDEDMIVTQSQTNFI---CPITQ---L 183

Query: 188 ELAEPIRSMECKHIYEKAAIMQYLKSK---KSRAQCPVAACPKM-LQPEKVVADPFLQIE 237
           E+ +P+++  C H YE+ AI++ ++SK   K +A CP   C    ++   ++ D  L+  
Sbjct: 184 EMKKPVKNKMCGHTYEEEAIVRMIESKHKRKKKACCPKIGCSHTDMRMSDLIPDEALRRA 243

[Close]
BLAST of Cla023353 vs. Swiss-Prot
Match: NSE2_XENLA (E3 SUMO-protein ligase NSE2 OS=Xenopus laevis GN=nsmce2 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-07
Identity = 53/202 (26.24%), Postives = 91/202 (45.05%), Query Frame = 1 

Query: 48  LERDNQSRMVKELENSVVELLSTYEDCNNFSSAIQSVGNMY--EPKEELTDFGKLLDDEV 107
           LE   +S  V  +E+ ++E  +   D   +  A++        E  E++ D   L+ ++ 
Sbjct: 43  LETGCESTEVDAMESVMLEYSAMERDLKQYIHAVEETVQKLRREQMEQVPDLQSLVQEKY 102

Query: 108 AKLSE-NSTSNLLNHSIIRQFREAIWNVHHS-GQPMPGEE-----QEDIVMTSTQCNLLN 167
           A + + N   +L  +    QF++ +  +    G+   G+       EDI +  +Q NL  
Sbjct: 103 ATIQKKNDDEDLKKNDRFVQFKDQLREMRKQMGEKEEGDAAFENVDEDIAVLPSQQNL-- 162

Query: 168 ITCPLSGKPVTELAEPIRSMECKHIYEKAAIMQYLK---SKKSRAQCPVAACPKM-LQPE 227
            TCP++     E+  P+++  C H YEK AI + ++    KK R +CP   C    +Q  
Sbjct: 163 -TCPITQM---EMTNPVKNKVCGHTYEKEAIERMIQDRHQKKKRVKCPKVGCVHSDMQIS 222

Query: 228 KVVADPFLQIEIDELRKM-SRH 236
            +V D  L+  ID L K   RH
Sbjct: 223 DLVPDTALKRTIDILNKQKGRH 238

[Close]
BLAST of Cla023353 vs. TrEMBL
Match: A0A0A0L3C3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G628940 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 3.9e-119
Identity = 215/248 (86.69%), Postives = 233/248 (93.95%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MASASDSRS+GV+GRIKSAATIMHS+NQSLLAELRK LIMMKEIGVDLE++ Q +MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSDNQSLLAELRKTLIMMKEIGVDLEKEKQYKMVKEL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           E S+VELLS YE+CNNFSSAIQSVGN YEPKEELTDF KLLDDEVAK+SE+S+SN  NH 
Sbjct: 61  EKSIVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSESSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           IIRQFREAIWNVHH+GQ M GEEQED+VMTSTQCNLLN+TCPLSGKPVTELAEP+RS+EC
Sbjct: 121 IIRQFREAIWNVHHAGQAMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSVEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSARIQ 240
           KHIYEKAAIMQYL SKKSRAQCPVAACPKMLQP+KVV DPFL+IEIDELRKMSRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSGRIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]
BLAST of Cla023353 vs. TrEMBL
Match: A0A067LBT9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15301 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.0e-86
Identity = 160/249 (64.26%), Postives = 200/249 (80.32%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS S SR  G +GRI++AA+   SE QSL+AE+RKAL MMKEI V  E+DN+S MVK+L
Sbjct: 1   MASTSASRDGGAAGRIRNAASTFSSEAQSLIAEIRKALNMMKEIAVCFEKDNESAMVKDL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           EN+V ELL +YEDC + +SAI+SVGN Y+P  ELTDF KLL+ E  KL  +S+    +H 
Sbjct: 61  ENAVAELLESYEDCQHHASAIESVGNSYQPGAELTDFKKLLEAEFEKLKSSSSFASQSHP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           ++RQFREAIWNVHHSGQPMPGEEQEDI+MTSTQ NLLNITCP+SGKPV ELAEP+RS++C
Sbjct: 121 LMRQFREAIWNVHHSGQPMPGEEQEDIIMTSTQSNLLNITCPVSGKPVIELAEPVRSVQC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSAR-- 240
           KHIYEK AIM ++ S K++A+CP+  CPK+LQ +KVV DP L +EI+E+R MSR +AR  
Sbjct: 181 KHIYEKKAIMHHIASGKAQAKCPITGCPKILQADKVVCDPLLLVEIEEMRSMSRQTARNN 240

Query: 241 -IQDFTELD 247
            ++DFTELD
Sbjct: 241 VVEDFTELD 249

[Close]
BLAST of Cla023353 vs. TrEMBL
Match: M5WIK7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010465mg PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.3e-85
Identity = 156/251 (62.15%), Postives = 198/251 (78.88%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS S SR+ G +GRI++A   ++ +NQSL++++R+A+  MKEI VDLERDNQ   VKEL
Sbjct: 1   MASTSASRTHGATGRIRTATATLYEDNQSLISDIRRAVGAMKEIAVDLERDNQFERVKEL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           EN+V+ELL T+EDC++FSS IQSVG  Y+P  ELTDF +L  +EVA L  NS S+  NH 
Sbjct: 61  ENAVIELLGTHEDCSHFSSTIQSVGEKYQPGPELTDFDQLFKNEVAMLKANSFSDPQNHP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           ++RQFREA+WNVHHSG+PMPGEEQEDIVMTS QCN+LN+TCPLSGKP+TEL  P+RS+ C
Sbjct: 121 LMRQFREAVWNVHHSGEPMPGEEQEDIVMTSNQCNILNVTCPLSGKPITELQHPVRSVVC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSAR-- 240
           KH+Y+K +IM YL+SK +R  CPVAACPK+LQ +KVV DP L +EIDELR M   +    
Sbjct: 181 KHVYDKGSIMHYLRSKNTR--CPVAACPKLLQADKVVCDPLLLVEIDELRAMKEQTVMTD 240

Query: 241 -IQDFTELDAD 249
            I+DFTELD +
Sbjct: 241 VIEDFTELDEE 249

[Close]
BLAST of Cla023353 vs. TrEMBL
Match: F6H212_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0014g02100 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 7.0e-84
Identity = 156/225 (69.33%), Postives = 189/225 (84.00%), Query Frame = 1 

Query: 27  NQSLLAELRKALIMMKEIGVDLERDNQSRMVKELENSVVELLSTYEDCNNFSSAIQSVGN 86
           N SLL + RKA+I+MKE+ VDLERD QS+MVKELE++VVELL T EDC  FS+AIQSVGN
Sbjct: 19  NLSLL-KFRKAMILMKEVAVDLERDGQSQMVKELEDAVVELLGTSEDCTQFSAAIQSVGN 78

Query: 87  MYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHSIIRQFREAIWNVHHSGQPMPGEEQED 146
            ++P+ ELTDF KL +DEVAKL  +S+    +H ++RQFREA+WNVHH+GQPMPG+EQED
Sbjct: 79  EHQPRPELTDFKKLFEDEVAKLKASSSFAPQSHPLLRQFREAVWNVHHAGQPMPGDEQED 138

Query: 147 IVMTSTQCNLLNITCPLSGKPVTELAEPIRSMECKHIYEKAAIMQYLKSKKSRAQCPVAA 206
           IVMTSTQCNLLNITCPLSG+PVTELA+P+RS++CKHIYEK AI+ Y+KSK  RAQCPVAA
Sbjct: 139 IVMTSTQCNLLNITCPLSGRPVTELADPVRSVDCKHIYEKKAILHYIKSKHGRAQCPVAA 198

Query: 207 CPKMLQPEKVVADPFLQIEIDELRKMSRHSAR---IQDFTELDAD 249
           CPK+LQ E+VV D  LQIEIDE++ MS+   R   I+DFTELD D
Sbjct: 199 CPKILQAERVVCDALLQIEIDEMKSMSKQPGRTDVIEDFTELDDD 242

[Close]
BLAST of Cla023353 vs. TrEMBL
Match: A0A061F8U4_THECC (RING/U-box superfamily protein, putative isoform 1 OS=Theobroma cacao GN=TCM_031645 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.2e-83
Identity = 153/249 (61.45%), Postives = 198/249 (79.52%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS S S S GVSGRI+ A+  + SENQ+L+ ++RKAL +MKEI VDLERDNQS MVK+L
Sbjct: 1   MASTSASLSDGVSGRIRRASPNLFSENQALVTDIRKALNLMKEIAVDLERDNQSEMVKQL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           E +V EL+  +E+C + SSAIQSVG  YEP  ELTDF KLLD E  K+  +S+S+  NH 
Sbjct: 61  EEAVAELVEAHENCLHHSSAIQSVGEAYEPGTELTDFKKLLDSEFEKVKASSSSSPQNHP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           +I QFR+A+WNVHH+GQPMPGEEQED+VMTST+ N+ N+ CPL+GKP+TEL EP+RS++C
Sbjct: 121 LIHQFRQAVWNVHHTGQPMPGEEQEDVVMTSTESNIKNLKCPLTGKPITELTEPVRSLDC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSAR-- 240
           KHIYEK AI+ ++KSK+  A+CPV+ACPKMLQ ++VV DP L  EI+E R +SR +AR  
Sbjct: 181 KHIYEKNAILDFIKSKRGNAKCPVSACPKMLQAKRVVCDPLLLFEIEEQRSLSRQTARTD 240

Query: 241 -IQDFTELD 247
            I+DFTE++
Sbjct: 241 VIEDFTEME 249

[Close]
BLAST of Cla023353 vs. NCBI nr
Match: gi|659127444|ref|XP_008463706.1| (PREDICTED: E3 SUMO-protein ligase MMS21 [Cucumis melo])

HSP 1 Score: 440.7 bits (1132), Expect = 1.7e-120
Identity = 218/248 (87.90%), Postives = 234/248 (94.35%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MASASDSRS+GV+GRIKSAATIMHSENQSLLAELRK LIMMKEIGVDLE++NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           E S+VELLS YE+CNNFSSAIQSVGN YEPKEELTDF KLLDDEVAK+S NS+SN  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           IIRQFREAIWNVHH+GQPM GEEQED+VMTSTQCNLLN+TCPLSGKPVTELAEP+RS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSARIQ 240
           KHIYEKAAIMQYL SKKSRAQCPVAACPKMLQP+KVV DPFL+IEIDELRKMSRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]
BLAST of Cla023353 vs. NCBI nr
Match: gi|449456611|ref|XP_004146042.1| (PREDICTED: E3 SUMO-protein ligase MMS21 [Cucumis sativus])

HSP 1 Score: 435.6 bits (1119), Expect = 5.6e-119
Identity = 215/248 (86.69%), Postives = 233/248 (93.95%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MASASDSRS+GV+GRIKSAATIMHS+NQSLLAELRK LIMMKEIGVDLE++ Q +MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSDNQSLLAELRKTLIMMKEIGVDLEKEKQYKMVKEL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           E S+VELLS YE+CNNFSSAIQSVGN YEPKEELTDF KLLDDEVAK+SE+S+SN  NH 
Sbjct: 61  EKSIVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSESSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           IIRQFREAIWNVHH+GQ M GEEQED+VMTSTQCNLLN+TCPLSGKPVTELAEP+RS+EC
Sbjct: 121 IIRQFREAIWNVHHAGQAMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSVEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSARIQ 240
           KHIYEKAAIMQYL SKKSRAQCPVAACPKMLQP+KVV DPFL+IEIDELRKMSRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSGRIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]
BLAST of Cla023353 vs. NCBI nr
Match: gi|731432107|ref|XP_010644169.1| (PREDICTED: E3 SUMO-protein ligase MMS21 isoform X1 [Vitis vinifera])

HSP 1 Score: 349.4 bits (895), Expect = 5.3e-93
Identity = 168/249 (67.47%), Postives = 211/249 (84.74%), Query Frame = 1 

Query: 3   SASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKELEN 62
           SA+ SR +GV GR+++AA+ ++S+NQSL+A +RKA+I+MKE+ VDLERD QS+MVKELE+
Sbjct: 5   SAAASRDNGVPGRLRNAASTLYSDNQSLIAVIRKAMILMKEVAVDLERDGQSQMVKELED 64

Query: 63  SVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHSII 122
           +VVELL T EDC  FS+AIQSVGN ++P+ ELTDF KL +DEVAKL  +S+    +H ++
Sbjct: 65  AVVELLGTSEDCTQFSAAIQSVGNEHQPRPELTDFKKLFEDEVAKLKASSSFAPQSHPLL 124

Query: 123 RQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMECKH 182
           RQFREA+WNVHH+GQPMPG+EQEDIVMTSTQCNLLNITCPLSG+PVTELA+P+RS++CKH
Sbjct: 125 RQFREAVWNVHHAGQPMPGDEQEDIVMTSTQCNLLNITCPLSGRPVTELADPVRSVDCKH 184

Query: 183 IYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSAR---I 242
           IYEK AI+ Y+KSK  RAQCPVAACPK+LQ E+VV D  LQIEIDE++ MS+   R   I
Sbjct: 185 IYEKKAILHYIKSKHGRAQCPVAACPKILQAERVVCDALLQIEIDEMKSMSKQPGRTDVI 244

Query: 243 QDFTELDAD 249
           +DFTELD D
Sbjct: 245 EDFTELDDD 253

[Close]
BLAST of Cla023353 vs. NCBI nr
Match: gi|1009161917|ref|XP_015899154.1| (PREDICTED: E3 SUMO-protein ligase MMS21 [Ziziphus jujuba])

HSP 1 Score: 340.1 bits (871), Expect = 3.2e-90
Identity = 162/251 (64.54%), Postives = 210/251 (83.67%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS+S SR  G++GRI+++A+ ++S++QSL+A++RKA  MMKE+ VDLERDNQS MVKEL
Sbjct: 1   MASSSASRRDGITGRIRNSASTLYSDSQSLIADIRKACSMMKEVAVDLERDNQSEMVKEL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           EN+VVELL TY +C +FSSAIQSVG  Y+P++ELTDF KL++DE+ KL  NS S   N+ 
Sbjct: 61  ENAVVELLDTYNNCMHFSSAIQSVGEKYQPEQELTDFKKLIEDEITKLKANSPSVPQNNP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           +IRQF+EA+WNVHH+GQ MPG+EQEDIVMTSTQCNLLNITCPL+GKPVTELA+P+RS+ C
Sbjct: 121 LIRQFKEAVWNVHHAGQLMPGDEQEDIVMTSTQCNLLNITCPLTGKPVTELADPVRSVGC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSAR-- 240
           KH+Y++ AI+QY++SK SR QCPVA CPK+LQ E VV D  L I+I E+R M+R +AR  
Sbjct: 181 KHVYDRKAILQYIRSKNSRGQCPVAGCPKILQEENVVCDTLLPIDIAEMRTMTRQTARTD 240

Query: 241 -IQDFTELDAD 249
            I+DFTE+D +
Sbjct: 241 VIEDFTEVDEE 251

[Close]
BLAST of Cla023353 vs. NCBI nr
Match: gi|802540405|ref|XP_012076268.1| (PREDICTED: E3 SUMO-protein ligase MMS21 [Jatropha curcas])

HSP 1 Score: 327.0 bits (837), Expect = 2.8e-86
Identity = 160/249 (64.26%), Postives = 200/249 (80.32%), Query Frame = 1 

Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS S SR  G +GRI++AA+   SE QSL+AE+RKAL MMKEI V  E+DN+S MVK+L
Sbjct: 1   MASTSASRDGGAAGRIRNAASTFSSEAQSLIAEIRKALNMMKEIAVCFEKDNESAMVKDL 60

Query: 61  ENSVVELLSTYEDCNNFSSAIQSVGNMYEPKEELTDFGKLLDDEVAKLSENSTSNLLNHS 120
           EN+V ELL +YEDC + +SAI+SVGN Y+P  ELTDF KLL+ E  KL  +S+    +H 
Sbjct: 61  ENAVAELLESYEDCQHHASAIESVGNSYQPGAELTDFKKLLEAEFEKLKSSSSFASQSHP 120

Query: 121 IIRQFREAIWNVHHSGQPMPGEEQEDIVMTSTQCNLLNITCPLSGKPVTELAEPIRSMEC 180
           ++RQFREAIWNVHHSGQPMPGEEQEDI+MTSTQ NLLNITCP+SGKPV ELAEP+RS++C
Sbjct: 121 LMRQFREAIWNVHHSGQPMPGEEQEDIIMTSTQSNLLNITCPVSGKPVIELAEPVRSVQC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVADPFLQIEIDELRKMSRHSAR-- 240
           KHIYEK AIM ++ S K++A+CP+  CPK+LQ +KVV DP L +EI+E+R MSR +AR  
Sbjct: 181 KHIYEKKAIMHHIASGKAQAKCPITGCPKILQADKVVCDPLLLVEIEEMRSMSRQTARNN 240

Query: 241 -IQDFTELD 247
            ++DFTELD
Sbjct: 241 VVEDFTELD 249

The following BLAST results are available for this feature:
BLAST of Cla023353 vs. Swiss-Prot
Match NameE-valueIdentityDescription
NSE2_ARATH2.0e-7657.38E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana GN=MMS21 PE=1 SV=1[more]
NSE2_HUMAN3.6e-0925.00E3 SUMO-protein ligase NSE2 OS=Homo sapiens GN=NSMCE2 PE=1 SV=2[more]
NSE2_BOVIN1.4e-0822.89E3 SUMO-protein ligase NSE2 OS=Bos taurus GN=NSMCE2 PE=2 SV=1[more]
NSE2_RAT9.0e-0824.60E3 SUMO-protein ligase NSE2 OS=Rattus norvegicus GN=Nsmce2 PE=2 SV=1[more]
NSE2_XENLA1.2e-0726.24E3 SUMO-protein ligase NSE2 OS=Xenopus laevis GN=nsmce2 PE=2 SV=1[more]
BLAST of Cla023353 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L3C3_CUCSA3.9e-11986.69Uncharacterized protein OS=Cucumis sativus GN=Csa_4G628940 PE=4 SV=1[more]
A0A067LBT9_JATCU2.0e-8664.26Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15301 PE=4 SV=1[more]
M5WIK7_PRUPE1.3e-8562.15Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010465mg PE=4 SV=1[more]
F6H212_VITVI7.0e-8469.33Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0014g02100 PE=4 SV=... [more]
A0A061F8U4_THECC1.2e-8361.45RING/U-box superfamily protein, putative isoform 1 OS=Theobroma cacao GN=TCM_031... [more]
BLAST of Cla023353 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659127444|ref|XP_008463706.1|1.7e-12087.90PREDICTED: E3 SUMO-protein ligase MMS21 [Cucumis melo][more]
gi|449456611|ref|XP_004146042.1|5.6e-11986.69PREDICTED: E3 SUMO-protein ligase MMS21 [Cucumis sativus][more]
gi|731432107|ref|XP_010644169.1|5.3e-9367.47PREDICTED: E3 SUMO-protein ligase MMS21 isoform X1 [Vitis vinifera][more]
gi|1009161917|ref|XP_015899154.1|3.2e-9064.54PREDICTED: E3 SUMO-protein ligase MMS21 [Ziziphus jujuba][more]
gi|802540405|ref|XP_012076268.1|2.8e-8664.26PREDICTED: E3 SUMO-protein ligase MMS21 [Jatropha curcas][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004181Znf_MIZ
IPR013083Znf_RING/FYVE/PHD
IPR026846Nse2(Mms21)
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0019789SUMO transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0000724double-strand break repair via homologous recombination
Vocabulary: Cellular Component
TermDefinition
GO:0030915Smc5-Smc6 complex
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0060250 germ-line stem-cell niche homeostasis
biological_process GO:0032876 negative regulation of DNA endoreduplication
biological_process GO:0008284 positive regulation of cell proliferation
biological_process GO:0080038 positive regulation of cytokinin-activated signaling pathway
biological_process GO:0045931 positive regulation of mitotic cell cycle
biological_process GO:0016925 protein sumoylation
biological_process GO:0010082 regulation of root meristem growth
cellular_component GO:0005634 nucleus
cellular_component GO:0030915 Smc5-Smc6 complex
molecular_function GO:0019789 SUMO transferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016874 ligase activity
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
WMU54895watermelon EST collection version 2.0transcribed_cluster
WMU60832watermelon EST collection version 2.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla023353Cla023353.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
WMU54895WMU54895transcribed_cluster
WMU60832WMU60832transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004181Zinc finger, MIZ-typePFAMPF11789zf-Nsecoord: 145..207
score: 2.1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 158..234
score: 4.
IPR026846E3 SUMO-protein ligase Nse2 (Mms21)PANTHERPTHR21330UNCHARACTERIZEDcoord: 1..248
score: 7.3E
NoneNo IPR availablePANTHERPTHR21330:SF2E3 SUMO-PROTEIN LIGASE MMS21coord: 1..248
score: 7.3E
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 159..231
score: 3.7