Cla015661 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla015661
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Polygalacturonase (AHRD V1 -- E3VSV7_CUCPE); contains Interpro domain(s) IPR000743 Glycoside hydrolase, family 28
Location	Chr2 : 2441132 .. 2442769 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTGATCATATCGAACTTCTTAGTTACTTCATTCTTGATGTCATTATTATCATTTACTATTGAATCAAAATCTCATGTTGATGTTATTTTTTCTTTGAGAAAATATGGTGACGTTGTGCCTGGTGTCGATATCACTGAGGTAAATACACAATAAAATGTATATCAAAGTGATTACTTATTGAATTGATCAATTGTGTTTTCGATAATTTTTGTTACTTTTGAAACATAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGTTCGTCCAAGTGCAATTGTTATTCCAAGAGGAATATTTAAAGTAAGTGAAGGAAAATTTAAAGGCCCTTGCAAGAGTCCTATTGAAATTCGACTTCGAGGAACTTTGCAAGCTCCAAAACACCCTCATGGAGATAGTTGGATTACTTTTGCATATGTTGATAGATTGACACTATCAGGTGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGGTTGGGAAAAGAATGATTGCCATAAAAACATAAATTGTGCTGACCTCCCTATTGTAAGCTTTATACCTTATATAGTTTTCCTTTCCAATCTCATTCATCTTTATTGATTCTTACAATTTGGTAGATGTTCTTGATACTTTATTACAATGTTTAAGGAATTATGTTTTAAAATAAATTTTAATCCTTAGAAAAATTAGGTAAGAAAGCTAAATTTTCAAAATATAAAGATTATATATATTAAGAGAAAACTCATGATTTTCCCCTCCCTATAAAAATATTTGGCTTCACTAAACTCTAACACAACATATTGATCATAAAAAATGAGCAGAGTTTAAGGTTCAACTTCATTACCAATTCAATTGTGAAGAGAATAACATCACTCGATAGCAAGAACTTCCATGTCAACATCCTAGGTTGTAACAACCTCACATTTCATGGTGTCAACATAATTGCACCGGAAAATAGTCCCAATACAGATGGAATACACATTGGCCGATCAATTGGAATCTCAATCACAAAATCTCGAATTTCAACCGGAGATGATTGCATCTCCCTTGGAGATGGAAGCAAACGAGTAAAAGTCACCAACGTGACATGCGGACCTGGACATGGAATAAGCATAGGAAGTCTTGGAAAATACACTAATGAAGAACCTGTTGAAGGTATCATAGTAAAGAATTGCACCAATTATGTTTTGAAGAAAAAAGCGAGGGCCAGCCTCTCCCGTTGCTGGCACTGCCACTAACATGCATTTTTCGGATATTATAATGGTCAATGTTAGCAACCCAATTCTCATAGACCAAGAATATTGCCCATGGAATCAGTGCAATCGCAAGGTATTAATCGCATTTTTAAAACTTTGATTTTTAAATTTCTTCATGTTAATTAACTTCACAAATGAACTTTTCATTTTAATTTTTCAGATTCCGTCAAAGATTAAGATTAGTAAAGTGAGCTTCAAGAATATTAAAGGGAGTTCTGCAACTCCAATGGCAGTGAAACTTATTTGTAGTAGTGACTTGCCATGTGACGAAGTAAAAGTAGCCAACATTGACCTGGTTTACAATGGAAAAGAAGGTCTCATTACATCTCAATGTACAAATGTGAGACCCATTATTTCGGGGATACAAAATCCTCAAACTTGCTCTTCCCCTTAA

mRNA sequence

ATGGCTGTGATCATATCGAACTTCTTAGTTACTTCATTCTTGATGTCATTATTATCATTTACTATTGAATCAAAATCTCATGTTGATGTTATTTTTTCTTTGAGAAAATATGGTGACGTTGTGCCTGGTGTCGATATCACTGAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGTTCGTCCAAGTGCAATTGTTATTCCAAGAGGAATATTTAAAGTAAGTGAAGGAAAATTTAAAGGCCCTTGCAAGAGTCCTATTGAAATTCGACTTCGAGGAACTTTGCAAGCTCCAAAACACCCTCATGGAGATAGTTGGATTACTTTTGCATATGTTGATAGATTGACACTATCAGGTGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGGTTGGGAAAAGAATGATTGCCATAAAAACATAAATTGTGCTGACCTCCCTATTCGAGGGCCAGCCTCTCCCGTTGCTGGCACTGCCACTAACATGCATTTTTCGGATATTATAATGGTCAATGTTAGCAACCCAATTCTCATAGACCAAGAATATTGCCCATGGAATCAGTGCAATCGCAAGATTCCGTCAAAGATTAAGATTAGTAAAGTGAGCTTCAAGAATATTAAAGGGAGTTCTGCAACTCCAATGGCAGTGAAACTTATTTGTAGTAGTGACTTGCCATGTGACGAAGTAAAAGTAGCCAACATTGACCTGGTTTACAATGGAAAAGAAGGTCTCATTACATCTCAATGTACAAATGTGAGACCCATTATTTCGGGGATACAAAATCCTCAAACTTGCTCTTCCCCTTAA

Coding sequence (CDS)

ATGGCTGTGATCATATCGAACTTCTTAGTTACTTCATTCTTGATGTCATTATTATCATTTACTATTGAATCAAAATCTCATGTTGATGTTATTTTTTCTTTGAGAAAATATGGTGACGTTGTGCCTGGTGTCGATATCACTGAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGTTCGTCCAAGTGCAATTGTTATTCCAAGAGGAATATTTAAAGTAAGTGAAGGAAAATTTAAAGGCCCTTGCAAGAGTCCTATTGAAATTCGACTTCGAGGAACTTTGCAAGCTCCAAAACACCCTCATGGAGATAGTTGGATTACTTTTGCATATGTTGATAGATTGACACTATCAGGTGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGGTTGGGAAAAGAATGATTGCCATAAAAACATAAATTGTGCTGACCTCCCTATTCGAGGGCCAGCCTCTCCCGTTGCTGGCACTGCCACTAACATGCATTTTTCGGATATTATAATGGTCAATGTTAGCAACCCAATTCTCATAGACCAAGAATATTGCCCATGGAATCAGTGCAATCGCAAGATTCCGTCAAAGATTAAGATTAGTAAAGTGAGCTTCAAGAATATTAAAGGGAGTTCTGCAACTCCAATGGCAGTGAAACTTATTTGTAGTAGTGACTTGCCATGTGACGAAGTAAAAGTAGCCAACATTGACCTGGTTTACAATGGAAAAGAAGGTCTCATTACATCTCAATGTACAAATGTGAGACCCATTATTTCGGGGATACAAAATCCTCAAACTTGCTCTTCCCCTTAA

Protein sequence

MAVIISNFLVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASVRPSAIVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDSWITFAYVDRLTLSGGGVFDGQGKAGWEKNDCHKNINCADLPIRGPASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSSATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSSP

Homology

[Close]

BLAST of Cla015661 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 9.6e-32
Identity = 63/110 (57.27%), Postives = 78/110 (70.91%), Query Frame = 1


Query: 157 GTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSSATPMAV 216
           G A+ MHF DI M +V  PILIDQ YCP+NQC  ++PS +K+SK+SFKNIKG+S T  AV
Sbjct: 246 GEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKEAV 305

Query: 217 KLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCS 267
           KL+CS   PC+ V++A+IDL Y+GK G  TS C N++P I G Q P  CS
Sbjct: 306 KLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICS 355

HSP 2 Score: 112.5 bits (280), Expect = 7.4e-24
Identity = 53/99 (53.54%), Postives = 65/99 (65.66%), Query Frame = 1


Query: 45  DITEALKDAWNDACASVRPSAIVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHP-- 104
           D T+AL  AW +ACAS  PS I++P+G F V     +GPCKS I ++L+GTL+AP  P  
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 105 -HGDSWITFAYVDRLTLSGGGVFDGQGKAGWEKNDCHKN 141
             G  WI    +D LT+ GGGVFDGQGK+ W +NDCHKN
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKN 99

[Close]

BLAST of Cla015661 vs. Swiss-Prot
Match: PGLR_BRANA (Polygalacturonase OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 9.6e-32
Identity = 71/158 (44.94%), Postives = 97/158 (61.39%), Query Frame = 1


Query: 120 GGGVFDGQ-GKAGWEKNDCHKNI-NCA------DLPIRG-PASPVAGTATNMHFSDIIMV 179
           G G+  G  G+ GWE++     + NC        L I+  P++    TA  +HF DII+ 
Sbjct: 232 GHGISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTTAAGIHFEDIILN 291

Query: 180 NVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSSATPMAVKLICSSDLPCDEVK 239
            VSNPILIDQEYCPWNQCN+  PS IK+  ++F+NI+G+S    AVKL+CS   PC+ V+
Sbjct: 292 KVSNPILIDQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDAVKLLCSKGHPCENVE 351

Query: 240 VANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSSP 269
           + +I++ Y G +G  T +CTNV P + G QNP+ C  P
Sbjct: 352 IGDINIEYTGPDGPPTFECTNVTPKLVGAQNPKACVGP 389

HSP 2 Score: 102.4 bits (254), Expect = 7.6e-21
Identity = 47/109 (43.12%), Postives = 62/109 (56.88%), Query Frame = 1


Query: 42  PGVDITEALKDAWNDACASVRPSAIVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAP-- 101
           P  DIT A+  A+  AC +  PS ++IP+G FK+ E    GPCKSPIE  L+G ++    
Sbjct: 31  PNSDITAAVLKAFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQGNVKTDGG 90

Query: 102 KHPHGDSWITFAYVDRLTLSGGGVFDGQGKAGWEKNDCHKNINCADLPI 149
                D W+ F  ++   L+GGG FDG+G A W+ N+CHK   C  LPI
Sbjct: 91  STQGKDRWVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPI 139

[Close]

BLAST of Cla015661 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.1e-31
Identity = 63/112 (56.25%), Postives = 79/112 (70.54%), Query Frame = 1


Query: 157 GTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSSATPMAV 216
           G  + +HF DI M NVS+PILIDQ+YCPWN+C +   SK+K+S +SFKNI+G+SA P A+
Sbjct: 288 GAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAI 347

Query: 217 KLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSSP 269
           K ICS   PC  V++A+ID+ +NG E   TSQC NV+PI SG  NP  CS P
Sbjct: 348 KFICSGSSPCQNVELADIDIKHNGAEP-ATSQCLNVKPITSGKLNPIPCSGP 398

HSP 2 Score: 115.9 bits (289), Expect = 6.7e-25
Identity = 58/144 (40.28%), Postives = 82/144 (56.94%), Query Frame = 1


Query: 9   LVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPG-VDITEALKDAWNDACASVRPSAIV 68
           +V S  + LL F   SK   D    + K+G    G  D+++   DAW +ACASV PS +V
Sbjct: 7   IVPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 69  IPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHG---DSWITFAYVDRLTLSGGGVF 128
           IP+G + +S+   +GPCK+PIEI ++GT+QAP  P      +W+ F  V+   + GGG+F
Sbjct: 67  IPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIF 126

Query: 129 DGQGKAGWEKNDCHKNINCADLPI 149
           DGQG   +EKN C      + LP+
Sbjct: 127 DGQGSIAYEKNTCENREFRSKLPV 150

[Close]

BLAST of Cla015661 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.1e-31
Identity = 58/111 (52.25%), Postives = 83/111 (74.77%), Query Frame = 1


Query: 157 GTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSSATPMAV 216
           G   ++HF DII+ NVSNP++IDQ YCP+N+CN+ +PS++KISKVSF+NIKG+S T  AV
Sbjct: 280 GVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAV 339

Query: 217 KLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSS 268
            L+ S  +PC+ ++V +ID+ Y+GKEG   S C N++P + G QNP  C++
Sbjct: 340 SLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENIKPSLKGKQNPPVCTA 390

HSP 2 Score: 106.7 bits (265), Expect = 4.0e-22
Identity = 52/120 (43.33%), Postives = 74/120 (61.67%), Query Frame = 1


Query: 31  IFSLRKYGDVVPGVDITEALKDAWNDACASVRPSAIVIPRGIFKVSEGKFKGPCKSPIEI 90
           +F + KYG      DI+EAL +A+ +AC S  PS IVIP+G F +++ K +GPCKSP+E+
Sbjct: 26  VFDITKYG-ANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKSPLEL 85

Query: 91  RLRGTLQAPKHPHG---DSWITFAYVDRLTLSGGGVFDGQGKAGWEKNDCHKNINCADLP 148
           +++ TL+AP  P       W+T   +D+ T+SGGG+ DGQ  A WE   C ++  C  LP
Sbjct: 86  QIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAWE---CKQSKKCNKLP 141

[Close]

BLAST of Cla015661 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 62/112 (55.36%), Postives = 78/112 (69.64%), Query Frame = 1


Query: 157 GTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSSATPMAV 216
           G  + +HF DI M NVS+PILIDQ+YCPWN+C +   SK+K+S +SFKNI+G+SA P A+
Sbjct: 288 GAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAI 347

Query: 217 KLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSSP 269
           K ICS   PC  V++A+ID+ +NG E   TSQC NV+PI  G  NP  CS P
Sbjct: 348 KFICSGSSPCQNVELADIDIQHNGAEP-ATSQCLNVKPITIGKLNPIPCSGP 398

HSP 2 Score: 115.9 bits (289), Expect = 6.7e-25
Identity = 58/144 (40.28%), Postives = 82/144 (56.94%), Query Frame = 1


Query: 9   LVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPG-VDITEALKDAWNDACASVRPSAIV 68
           +V S  + LL F   SK   D    + K+G    G  D+++   DAW +ACASV PS +V
Sbjct: 7   IVPSMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 69  IPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHG---DSWITFAYVDRLTLSGGGVF 128
           IP+G + +S+   +GPCK+PIEI ++GT+QAP  P      +W+ F  V+   + GGG+F
Sbjct: 67  IPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIF 126

Query: 129 DGQGKAGWEKNDCHKNINCADLPI 149
           DGQG   +EKN C      + LP+
Sbjct: 127 DGQGSIAYEKNTCENREFRSKLPV 150

[Close]

BLAST of Cla015661 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.9e-63
Identity = 117/140 (83.57%), Postives = 129/140 (92.14%), Query Frame = 1


Query: 9   LVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASVRPSAIVI 68
           LVT+FLMSLLSFTIE++SHVD IFSLRKYG++V G D+TEALKDAWNDACAS RPSA+VI
Sbjct: 10  LVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVI 69

Query: 69  PRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDSWITFAYVDRLTLSGGGVFDGQG 128
           P+G FKV EG+FKGPCKSPIE R+ GTLQAPKHPHGDSWITFAYVDR+ LSGGGVFDGQG
Sbjct: 70  PKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGGGVFDGQG 129

Query: 129 KAGWEKNDCHKNINCADLPI 149
           KAGWEKNDCHK I+CA LPI
Sbjct: 130 KAGWEKNDCHKRIDCAKLPI 148

[Close]

BLAST of Cla015661 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.8e-51
Identity = 100/117 (85.47%), Postives = 109/117 (93.16%), Query Frame = 1


Query: 151 PASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSS 210
           PASPVAG ATNMHFSDI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNI+GSS
Sbjct: 281 PASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSS 340

Query: 211 ATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSS 268
           ATP+AVKLICSS+LPC+EVKVANIDLVYNG +G ITS+C NV+PIISGIQNP  CSS
Sbjct: 341 ATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICSS 397

HSP 2 Score: 171.0 bits (432), Expect = 1.9e-39
Identity = 77/118 (65.25%), Postives = 97/118 (82.20%), Query Frame = 1


Query: 151 PASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSS 210
           P S V+  A++MHF DI M NV+NPI+IDQEYCPWNQCNRK+PS IKISKVSFKNI+G+S
Sbjct: 289 PDSAVSYPASDMHFEDIEMENVANPIIIDQEYCPWNQCNRKVPSAIKISKVSFKNIRGTS 348

Query: 211 ATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSSP 269
           +TP+AVKL+CS   PC+ V++A+IDL Y+GKEG   S+C+NV+P ISG QNP  C+ P
Sbjct: 349 STPVAVKLVCSKSNPCEGVEIADIDLTYSGKEGPAVSECSNVKPTISGKQNPPICAKP 406

[Close]

BLAST of Cla015661 vs. TrEMBL
Match: E3VSV7_CUCPE (Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.8e-30
Identity = 71/148 (47.97%), Postives = 95/148 (64.19%), Query Frame = 1


Query: 4   IISNFLVTSFLMSLL--SFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASV 63
           ++S F+    L+ LL  + T+ + S V   F +  +G   P  D T+AL DAW +ACAS 
Sbjct: 13  LLSKFMALIPLLLLLVSNATVGATSGV---FDITTFG-AKPNADATQALADAWKEACASA 72

Query: 64  RPSAIVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQA-PKHPHGDSWITFAYVDRLTLSG 123
            PS +++P+G +++ E + KGPCKSPIE+ + GTLQA P    GD  +   Y+D LTLSG
Sbjct: 73  TPSKVLVPKGTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYIDHLTLSG 132

Query: 124 GGVFDGQGKAGWEKNDCHKNINCADLPI 149
            GVFDGQGKAGWEKNDCH    C  LP+
Sbjct: 133 TGVFDGQGKAGWEKNDCHLKKECKKLPM 156

HSP 2 Score: 169.1 bits (427), Expect = 7.4e-39
Identity = 98/251 (39.04%), Postives = 145/251 (57.77%), Query Frame = 1


Query: 12  SFLMSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASVRPSAIVIPRG 71
           +F++ LL   + S      +  ++K+G   P  DIT+AL  AW++ACAS   + IVIP G
Sbjct: 7   TFVLFLLLTILGSTQ--SAVIDIKKFGGA-PNADITQALTKAWDEACASTTATKIVIPGG 66

Query: 72  IFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDS----WITFAYVDRLTLSGGGVFDGQ 131
            +K++  + KGPCK+PIEI + G +QAP  P+  +    WI   +VD LT+SG GVFDGQ
Sbjct: 67  TYKMNAIQVKGPCKAPIEINVAGIIQAPPDPNAMNDAYEWIKIEHVDFLTISGHGVFDGQ 126

Query: 132 GKAGWEKNDCHKNINCADLPIRGPASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQ 191
           G   W++NDC  N  C    ++                   +  ++N + +      W  
Sbjct: 127 GAKAWKENDCGTNQKCKKRSLQH------------------LTKLANELNLF-----W-- 186

Query: 192 CNRKIPSKIKISKVSFKNIKGSSATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITS 251
                PSKIKISKV+FKNIKG+S T   V L+CSS +PCD V++++I+L +NG    I +
Sbjct: 187 ----APSKIKISKVTFKNIKGTSKTKEGVILMCSSGVPCDGVELSDINLTFNGAP--IDA 223

Query: 252 QCTNVRPIISG 259
           +C NV+P+I+G
Sbjct: 247 KCANVKPLITG 223

[Close]

BLAST of Cla015661 vs. TrEMBL
Match: A0A0D2NBK5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G193800 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.8e-38
Identity = 76/117 (64.96%), Postives = 96/117 (82.05%), Query Frame = 1


Query: 151 PASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSS 210
           PAS   G+AT++HF DI M NV NPIL+DQEYCPWNQC+  +PS++++SK+SFKNI+G+S
Sbjct: 277 PASQ-PGSATDIHFEDITMDNVGNPILVDQEYCPWNQCDLSVPSRVQLSKLSFKNIRGTS 336

Query: 211 ATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSS 268
            T +AVKLICSS LPCDEV++A+IDL YNG EG   SQC+NV P +SG+QNP  CSS
Sbjct: 337 KTQIAVKLICSSGLPCDEVELADIDLTYNGPEGPAISQCSNVHPALSGVQNPAACSS 392

[Close]

BLAST of Cla015661 vs. TrEMBL
Match: A0A0D2NBK5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G193800 PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-26
Identity = 64/146 (43.84%), Postives = 89/146 (60.96%), Query Frame = 1


Query: 7   NFLVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPG-VDITEALKDAWNDACASVRPSA 66
           N  +T+ L+      I   + +DV+    K+G V  G  D++  L +AW +ACAS  P  
Sbjct: 2   NIAITALLLLFTLIPIVECTDIDVV---AKFGAVADGQTDLSLPLMNAWKEACASPEPVN 61

Query: 67  IVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHP---HGDSWITFAYVDRLTLSGGG 126
           IVIP G + +SE    GPC++PI ++L+GTL AP+ P      +W++ +YVD LT+ GGG
Sbjct: 62  IVIPEGTYLLSEATLNGPCQAPISLQLQGTLMAPEDPTVFKEPTWVSISYVDSLTMFGGG 121

Query: 127 VFDGQGKAGWEKNDCHKNINCADLPI 149
           VFDGQG   W +NDC KN NC  LPI
Sbjct: 122 VFDGQGATAWGQNDCSKNKNCVKLPI 144

HSP 2 Score: 162.5 bits (410), Expect = 6.9e-37
Identity = 71/111 (63.96%), Postives = 89/111 (80.18%), Query Frame = 1


Query: 157 GTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSSATPMAV 216
           G A+N+HF DI+M NV NP+LIDQEYCPWNQCN K+PS+IKIS VSFKNI+G+S T +AV
Sbjct: 284 GLASNLHFEDIVMENVGNPVLIDQEYCPWNQCNAKVPSRIKISDVSFKNIRGTSTTALAV 343

Query: 217 KLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSS 268
            L+CSS  PC  V++A+IDL Y+G +G I SQC NV+P ISG QNP  C++
Sbjct: 344 NLVCSSGFPCQNVEIADIDLQYSGNDGPIMSQCKNVKPTISGKQNPNPCAT 394

[Close]

BLAST of Cla015661 vs. NCBI nr
Match: gi|659111053|ref|XP_008455553.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 261.2 bits (666), Expect = 2.1e-66
Identity = 120/149 (80.54%), Postives = 139/149 (93.29%), Query Frame = 1


Query: 1   MAVI-ISNFLVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACA 60
           M+VI  S+ LV +FLM+LLSFT+ES+SHVDVIF LRKYG++V G D+TEALKDAWNDACA
Sbjct: 1   MSVIKYSSLLVITFLMALLSFTVESRSHVDVIFHLRKYGNIVSGADVTEALKDAWNDACA 60

Query: 61  SVRPSAIVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDSWITFAYVDRLTLS 120
           S+RPSA+VIP+G FKV EG+FKGPCKSP+E+R+RGTLQAPKHPHGDSWITFAYVDR+TLS
Sbjct: 61  SLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWITFAYVDRMTLS 120

Query: 121 GGGVFDGQGKAGWEKNDCHKNINCADLPI 149
           GGGVFDGQGKAGWEKNDCHK+I+CA LPI
Sbjct: 121 GGGVFDGQGKAGWEKNDCHKHISCARLPI 149

[Close]

BLAST of Cla015661 vs. NCBI nr
Match: gi|659111053|ref|XP_008455553.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 208.4 bits (529), Expect = 1.6e-50
Identity = 99/117 (84.62%), Postives = 108/117 (92.31%), Query Frame = 1


Query: 151 PASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSS 210
           PASPVAG ATNMHF DI MVNVSNPILIDQEYCPWNQCNRKIPSKIKI+KVSFKNI+GSS
Sbjct: 282 PASPVAGIATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSS 341

Query: 211 ATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSS 268
           ATP+AVKLICSS+LPC+EVKVANIDLVYNG +G +TS+C NV+PIISG QNP  CSS
Sbjct: 342 ATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPICSS 398

HSP 2 Score: 250.8 bits (639), Expect = 2.8e-63
Identity = 117/140 (83.57%), Postives = 129/140 (92.14%), Query Frame = 1


Query: 9   LVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASVRPSAIVI 68
           LVT+FLMSLLSFTIE++SHVD IFSLRKYG++V G D+TEALKDAWNDACAS RPSA+VI
Sbjct: 10  LVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVI 69

Query: 69  PRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDSWITFAYVDRLTLSGGGVFDGQG 128
           P+G FKV EG+FKGPCKSPIE R+ GTLQAPKHPHGDSWITFAYVDR+ LSGGGVFDGQG
Sbjct: 70  PKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGGGVFDGQG 129

Query: 129 KAGWEKNDCHKNINCADLPI 149
           KAGWEKNDCHK I+CA LPI
Sbjct: 130 KAGWEKNDCHKRIDCAKLPI 148

[Close]

BLAST of Cla015661 vs. NCBI nr
Match: gi|700188231|gb|KGN43464.1| (hypothetical protein Csa_7G037910 [Cucumis sativus])

HSP 1 Score: 209.9 bits (533), Expect = 5.4e-51
Identity = 100/117 (85.47%), Postives = 109/117 (93.16%), Query Frame = 1


Query: 151 PASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSS 210
           PASPVAG ATNMHFSDI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNI+GSS
Sbjct: 281 PASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSS 340

Query: 211 ATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSS 268
           ATP+AVKLICSS+LPC+EVKVANIDLVYNG +G ITS+C NV+PIISGIQNP  CSS
Sbjct: 341 ATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICSS 397

HSP 2 Score: 241.5 bits (615), Expect = 1.7e-60
Identity = 112/134 (83.58%), Postives = 123/134 (91.79%), Query Frame = 1


Query: 15  MSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASVRPSAIVIPRGIFK 74
           MSLLSFTIE++SHVD IFSLRKYG++V G D+TEALKDAWNDACAS RPSA+VIP+G FK
Sbjct: 1   MSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVIPKGTFK 60

Query: 75  VSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDSWITFAYVDRLTLSGGGVFDGQGKAGWEK 134
           V EG+FKGPCKSPIE R+ GTLQAPKHPHGDSWITFAYVDR+ LSGGGVFDGQGKAGWEK
Sbjct: 61  VREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGGGVFDGQGKAGWEK 120

Query: 135 NDCHKNINCADLPI 149
           NDCHK I+CA LPI
Sbjct: 121 NDCHKRIDCAKLPI 133

[Close]

BLAST of Cla015661 vs. NCBI nr
Match: gi|778730103|ref|XP_011659699.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 209.9 bits (533), Expect = 5.4e-51
Identity = 100/117 (85.47%), Postives = 109/117 (93.16%), Query Frame = 1


Query: 151 PASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIKGSS 210
           PASPVAG ATNMHFSDI MVNVSNPILIDQEYCPWNQCNR+IPS IKI+KVSFKNI+GSS
Sbjct: 266 PASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSS 325

Query: 211 ATPMAVKLICSSDLPCDEVKVANIDLVYNGKEGLITSQCTNVRPIISGIQNPQTCSS 268
           ATP+AVKLICSS+LPC+EVKVANIDLVYNG +G ITS+C NV+PIISGIQNP  CSS
Sbjct: 326 ATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICSS 382

HSP 2 Score: 239.6 bits (610), Expect = 6.4e-60
Identity = 114/143 (79.72%), Postives = 126/143 (88.11%), Query Frame = 1


Query: 6   SNFLVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASVRPSA 65
           SN  +T+FLMSLLSFTIES+SHVDVIF LRKYG++        ALKDAWNDACASVRPSA
Sbjct: 7   SNLSITTFLMSLLSFTIESQSHVDVIFPLRKYGNI--------ALKDAWNDACASVRPSA 66

Query: 66  IVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDSWITFAYVDRLTLSGGGVFD 125
           +VI  GIFKV EG+FKGPCKSPIEIR+RGTLQAPKHPHGDSWITFAYVDR+T+SG GVFD
Sbjct: 67  VVIRDGIFKVREGEFKGPCKSPIEIRVRGTLQAPKHPHGDSWITFAYVDRMTISGKGVFD 126

Query: 126 GQGKAGWEKNDCHKNINCADLPI 149
           GQGKAGWEKNDCHK I+C +LPI
Sbjct: 127 GQGKAGWEKNDCHKRIDCVNLPI 141

[Close]

BLAST of Cla015661 vs. NCBI nr
Match: gi|778730075|ref|XP_011659692.1| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 86.3 bits (212), Expect = 9.0e-14
Identity = 37/42 (88.10%), Postives = 40/42 (95.24%), Query Frame = 1


Query: 151 PASPVAGTATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKI 193
           P+SPVAG ATNMHFSDI MVNVSNPILIDQEYCPWNQCNR++
Sbjct: 274 PSSPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREV 315

HSP 2 Score: 234.2 bits (596), Expect = 2.7e-58
Identity = 108/127 (85.04%), Postives = 119/127 (93.70%), Query Frame = 1


Query: 6   SNFLVTSFLMSLLSFTIESKSHVDVIFSLRKYGDVVPGVDITEALKDAWNDACASVRPSA 65
           SN LVT+FLMSLLSFTIES+SH+DVIF L KYG++V G D+TEALKDAWNDACASV PSA
Sbjct: 7   SNLLVTTFLMSLLSFTIESRSHIDVIFPLEKYGNIVSGADVTEALKDAWNDACASVHPSA 66

Query: 66  IVIPRGIFKVSEGKFKGPCKSPIEIRLRGTLQAPKHPHGDSWITFAYVDRLTLSGGGVFD 125
           +VIP+GIFKV EGKFKGPCKSPIEIR+RGTLQAPKHP+GDSWITFAYVDR+TLSGGGVFD
Sbjct: 67  VVIPKGIFKVREGKFKGPCKSPIEIRVRGTLQAPKHPYGDSWITFAYVDRMTLSGGGVFD 126

Query: 126 GQGKAGW 133
           GQGK GW
Sbjct: 127 GQGKEGW 133

The following BLAST results are available for this feature:

BLAST of Cla015661 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR_OENOR	9.6e-32	57.27	Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1	[more]
PGLR_BRANA	9.6e-32	44.94	Polygalacturonase OS=Brassica napus PE=2 SV=1	[more]
PGLR_GOSHI	2.1e-31	56.25	Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1	[more]
PGLR_TOBAC	8.1e-31	52.25	Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1	[more]
PGLR_GOSBA	1.1e-30	55.36	Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1	[more]

BLAST of Cla015661 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3B7_CUCSA	1.9e-63	83.57	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1	[more]
A0A0A0K3B7_CUCSA	3.8e-51	85.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1	[more]
E3VSV7_CUCPE	2.8e-30	47.97	Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1	[more]
A0A0D2NBK5_GOSRA	2.8e-38	64.96	Uncharacterized protein OS=Gossypium raimondii GN=B456_001G193800 PE=3 SV=1	[more]
A0A0D2NBK5_GOSRA	1.1e-26	43.84	Uncharacterized protein OS=Gossypium raimondii GN=B456_001G193800 PE=3 SV=1	[more]

BLAST of Cla015661 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659111053\|ref\|XP_008455553.1\|	2.1e-66	80.54	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
gi\|659111053\|ref\|XP_008455553.1\|	1.6e-50	84.62	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
gi\|700188231\|gb\|KGN43464.1\|	5.4e-51	85.47	hypothetical protein Csa_7G037910 [Cucumis sativus]	[more]
gi\|778730103\|ref\|XP_011659699.1\|	5.4e-51	85.47	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|778730075\|ref\|XP_011659692.1\|	9.0e-14	88.10	PREDICTED: polygalacturonase-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000743	Glyco_hydro_28
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla015661	Cla015661.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 155..253 score: 7.9E-25coord: 58..147 score: 1.5
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 18..266 score: 1.11
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 44..144 score: 2.9E-20coord: 157..266 score: 1.1
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 8..267 score: 3.2
None	No IPR available	PANTHER	PTHR31375:SF37	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 8..267 score: 3.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla015661	MELO3C024218	Melon (DHL92) v3.5.1	mewmB241
Cla015661	Cucsa.100530	Cucumber (Gy14) v1	cgywmB130
Cla015661	Lsi11G013700	Bottle gourd (USVL1VR-Ls)	lsiwmB106
Cla015661	MELO3C024218.2	Melon (DHL92) v3.6.1	medwmB233

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla015661	Cucurbita pepo (Zucchini)	cpewmB268
Cla015661	Cucurbita pepo (Zucchini)	cpewmB305
Cla015661	Cucurbita pepo (Zucchini)	cpewmB675
Cla015661	Bottle gourd (USVL1VR-Ls)	lsiwmB080
Cla015661	Bottle gourd (USVL1VR-Ls)	lsiwmB105
Cla015661	Cucumber (Gy14) v2	cgybwmB484
Cla015661	Cucumber (Gy14) v2	cgybwmB565
Cla015661	Cucumber (Gy14) v2	cgybwmB566
Cla015661	Melon (DHL92) v3.6.1	medwmB109
Cla015661	Silver-seed gourd	carwmB0194
Cla015661	Silver-seed gourd	carwmB0334
Cla015661	Silver-seed gourd	carwmB1066
Cla015661	Cucumber (Chinese Long) v3	cucwmB541
Cla015661	Cucumber (Chinese Long) v3	cucwmB633
Cla015661	Cucumber (Chinese Long) v3	cucwmB634
Cla015661	Watermelon (97103) v2	wmwmbB317
Cla015661	Watermelon (97103) v2	wmwmbB333
Cla015661	Wax gourd	wgowmB272
Cla015661	Wax gourd	wgowmB403
Cla015661	Cucumber (Gy14) v1	cgywmB186
Cla015661	Cucurbita maxima (Rimu)	cmawmB121
Cla015661	Cucurbita maxima (Rimu)	cmawmB503
Cla015661	Cucurbita maxima (Rimu)	cmawmB527
Cla015661	Cucurbita moschata (Rifu)	cmowmB117
Cla015661	Cucurbita moschata (Rifu)	cmowmB496
Cla015661	Melon (DHL92) v3.5.1	mewmB110
Cla015661	Watermelon (Charleston Gray)	wcgwmB079
Cla015661	Watermelon (Charleston Gray)	wcgwmB198
Cla015661	Watermelon (Charleston Gray)	wcgwmB215
Cla015661	Cucumber (Chinese Long) v2	cuwmB603
Cla015661	Cucumber (Chinese Long) v2	cuwmB604