Cucsa.100530 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.100530 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.2e-98
Identity = 182/393 (46.31%), Postives = 252/393 (64.12%), Query Frame = 1

Query: 9   VTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSG-VDVTEALKDAWNDACASVPPSAVII 68
           V +  ++LL F   S+   D    + K+G    G  D+++   DAW +ACASV PS V+I
Sbjct: 8   VPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVI 67

Query: 69  PKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHP---QGDSWITFVYVDRMKL-SGGVFD 128
           PKG + + +   +GPCK+PIE+ V+GT+QA   P   +  +W+ F  V+  K+  GG+FD
Sbjct: 68  PKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFD 127

Query: 129 GQGKEGWR-----------------------------ITSLDSKNSHVNILGCNNLTFQG 188
           GQG   +                              ITS DSK  H+N+  C N+T + 
Sbjct: 128 GQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLER 187

Query: 189 VNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPGHGIS 248
           + I AP++SPNTD IH+G+S  ++I+ S I  GDDCIS+G+G+K + + ++TCGPGHGIS
Sbjct: 188 LKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGIS 247

Query: 249 IGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMINVSNP 308
           IGSLGK+ NEEPVEG+ + NCTI NT+NG RIKTW       ++ I HF DITM NVS+P
Sbjct: 248 IGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEI-HFEDITMNNVSSP 307

Query: 309 ILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKVANID 368
           ILIDQ+YCPWN+C +   SK+K++ +SFKNIRG SA   A+K ICS + PC+ V++A+ID
Sbjct: 308 ILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADID 367

BLAST of Cucsa.100530 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.1e-97
Identity = 181/393 (46.06%), Postives = 251/393 (63.87%), Query Frame = 1

Query: 9   VTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSG-VDVTEALKDAWNDACASVPPSAVII 68
           V +  ++LL F   S+   D    + K+G    G  D+++   DAW +ACASV PS V+I
Sbjct: 8   VPSMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVI 67

Query: 69  PKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHP---QGDSWITFVYVDRMKL-SGGVFD 128
           PKG + + +   +GPCK+PIE+ V+GT+QA   P   +  +W+ F  V+  K+  GG+FD
Sbjct: 68  PKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFD 127

Query: 129 GQGKEGWR-----------------------------ITSLDSKNSHVNILGCNNLTFQG 188
           GQG   +                              ITS DSK  H+N+  C N+T + 
Sbjct: 128 GQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLER 187

Query: 189 VNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPGHGIS 248
           + I AP++SPNTD IH+G+S  ++I+ S I  GDDCIS+G+G+K + + ++TCGPGHGIS
Sbjct: 188 LKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGIS 247

Query: 249 IGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMINVSNP 308
           IGSLGK+ NEEPVEG+ + NCTI NT+NG RIKTW       ++ I HF DITM NVS+P
Sbjct: 248 IGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEI-HFEDITMNNVSSP 307

Query: 309 ILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKVANID 368
           ILIDQ+YCPWN+C +   SK+K++ +SFKNIRG SA   A+K ICS + PC+ V++A+ID
Sbjct: 308 ILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADID 367

BLAST of Cucsa.100530 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 7.0e-97
Identity = 180/356 (50.56%), Postives = 231/356 (64.89%), Query Frame = 1

Query: 44  DVTEALKDAWNDACASVPPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHP-- 103
           D T+AL  AW +ACAS  PS +++PKG F V     +GPCKS I ++++GTL+A   P  
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 104 -QGDSWITFVYVDRMKL-SGGVFDGQGKEGW----------------------------- 163
            +G  WI    +D + +  GGVFDGQGK  W                             
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 164 -RITSLDSKNSHVNILGCNNLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDD 223
             +T+LDSKN HVN++GC NLTF+   I A E S NTD IHIGRS  ++I+ + I  GDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 224 CISLGNGSKRVKVTDVTCGPGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTW 283
           CISLG+GSK + +T++TCGPGHGIS+GSLG+Y NEE V G+ VKNCTI  + NGVRIKTW
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 284 SASPVAGIAIIMHFSDITMINVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCS 343
             S   G A  MHF DITM +V  PILIDQ YCP+NQC  ++PS +K++K+SFKNI+G S
Sbjct: 241 PKSE-PGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 344 ATSVAVKLICSSNLPCEEVKVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICS 366
            T  AVKL+CS + PC  V++A+IDL Y+G  G  TS C N+KP + G Q P+ICS
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICS 355

BLAST of Cucsa.100530 vs. Swiss-Prot
Match: PGLR_MEDSA (Polygalacturonase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.7e-96
Identity = 180/404 (44.55%), Postives = 252/404 (62.38%), Query Frame = 1

Query: 2   IKYSSLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASVP 61
           +K+S+  + +FL I      +S      +  + K+G   +  D+ +AL  AWN+ACAS  
Sbjct: 1   MKFSTAIIVSFLFIADFCAAQSG-----VLDISKFGGKPNS-DIGQALTSAWNEACASTT 60

Query: 62  PSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHPQ----GDSWITFVYVDRMKL 121
            + ++IP G +++   E KGPCK+PIE++V GT+QA   P      + W  F+Y+D + L
Sbjct: 61  AAKIVIPAGTYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTL 120

Query: 122 SG-GVFDGQGKEGWR---------------------------------ITSLDSKNSHVN 181
           SG GVFDGQG   ++                                 +TS DSKN HV 
Sbjct: 121 SGKGVFDGQGATVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVM 180

Query: 182 ILGCNNLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVT 241
           + GC N+TF G  I AP DSPNTD IH+G+S  + IL + I  GDDC+S+G+GSK++ V 
Sbjct: 181 VFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQ 240

Query: 242 DVTCGPGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHF 301
            V CGPGHG+S+GSLGK+T EE VEG+ VKNCT+  T NGVRIKTW  +P       +HF
Sbjct: 241 GVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHF 300

Query: 302 SDITMINVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNL 361
            DITM NV NP++IDQEY PWNQC+++ PSKIK++K+SFKN++G S T+  V LICSS +
Sbjct: 301 EDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAV 360

Query: 362 PCEEVKVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSSP 368
           PC+ V++ N+DL +NG     T++C NVKP+++G     +C +P
Sbjct: 361 PCDGVELNNVDLKFNG--APTTAKCTNVKPLVTG--TAPVCQAP 394

BLAST of Cucsa.100530 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 4.0e-92
Identity = 173/385 (44.94%), Postives = 245/385 (63.64%), Query Frame = 1

Query: 12  FLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASVPPSAVIIPKGI 71
           F ++LL       S   V F + KYG   S  D++EAL +A+ +AC S  PS ++IPKG 
Sbjct: 9   FALVLLFLAHFGESQTGV-FDITKYG-ANSNADISEALLNAFKEACQSTSPSTIVIPKGT 68

Query: 72  FKVREGEFKGPCKSPIEMRVRGTLQALNHPQG---DSWITFVYVDRMKLSGG-VFDGQGK 131
           F + + + +GPCKSP+E++++ TL+A + P       W+T   +D+  +SGG + DGQ  
Sbjct: 69  FTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAA 128

Query: 132 EGWR--------------------------ITSLDSKNSHVNILGCNNLTFQGVNIIAPE 191
             W                           IT+LDSK+ HVN+  C NLTF   N+ AP 
Sbjct: 129 AAWECKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPA 188

Query: 192 DSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPGHGISIGSLGKY 251
           +SPNTD IH+ RS  ++I  S  + GDDCIS+G+ ++++ +T VTCGPGHGIS+GSLG  
Sbjct: 189 NSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGN 248

Query: 252 TNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMINVSNPILIDQEY 311
            +E+PV GV V+NCT  NT NGVRIKTW AS   G+   +HF DI + NVSNP++IDQ Y
Sbjct: 249 PDEKPVVGVFVRNCTFTNTDNGVRIKTWPASH-PGVVNDVHFEDIIVQNVSNPVVIDQVY 308

Query: 312 CPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKVANIDLVYNGTK 367
           CP+N+CN+ +PS++KI+KVSF+NI+G S T  AV L+ S  +PCE ++V +ID+ Y+G +
Sbjct: 309 CPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKE 368

BLAST of Cucsa.100530 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 2.5e-178
Identity = 323/403 (80.15%), Postives = 338/403 (83.87%), Query Frame = 1

Query: 1   MIKYSSLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASV 60
           +IK+  L VTTFLM LLSFTIE+RSHVD IF LRKYGNIVSG DVTEALKDAWNDACAS 
Sbjct: 3   VIKHLILLVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASD 62

Query: 61  PPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHPQGDSWITFVYVDRMKLSGG 120
            PSAV+IPKG FKVREGEFKGPCKSPIE RV GTLQA  HP GDSWITF YVDRM+LSGG
Sbjct: 63  RPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGG 122

Query: 121 -VFDGQGKEGW-----------------------------RITSLDSKNSHVNILGCNNL 180
            VFDGQGK GW                             RITSLDSKN HVNILGCNNL
Sbjct: 123 GVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNFHVNILGCNNL 182

Query: 181 TFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPG 240
           TFQGVNIIAPE+SPNTD IHIGRSI ISILKSRIA GDDCISLG+GSKR+KV +V CGPG
Sbjct: 183 TFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRIKVNNVICGPG 242

Query: 241 HGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMIN 300
           HGISIGSLGKY NEEPVEGV+V NCTI+NTTNGVRIKTW ASPVAGIA  MHFSDITM+N
Sbjct: 243 HGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATNMHFSDITMVN 302

Query: 301 VSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKV 360
           VSNPILIDQEYCPWNQCNR+IPS IKINKVSFKNIRG SAT VAVKLICSSNLPCEEVKV
Sbjct: 303 VSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKV 362

Query: 361 ANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSSPYTIASK 374
           ANIDLVYNG KGSITS+CMNVKPI+SGIQNP ICSS YT ASK
Sbjct: 363 ANIDLVYNGIKGSITSECMNVKPIISGIQNPPICSSSYTTASK 404

BLAST of Cucsa.100530 vs. TrEMBL
Match: W9SC16_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_020041 PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 3.8e-110
Identity = 206/396 (52.02%), Postives = 267/396 (67.42%), Query Frame = 1

Query: 2   IKYSSLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASVP 61
           +K S+  +   L+++ S    ++    V+F + + G I+   D+T+AL  AWN+ACAS  
Sbjct: 3   LKLSNFVIAALLLLVSSNIANAQI---VLFDVTENGTILPQSDITDALLKAWNNACASTI 62

Query: 62  PSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQAL----NHPQGDSWITFVYVDRMKL 121
           PS V++PKGIFK+     +GPC +PIE  ++G LQA     N   GD+W+ F YVD + +
Sbjct: 63  PSKVVVPKGIFKLSGATLEGPCNAPIEFDLQGILQAPPEDGNFTGGDTWVAFEYVDLLTI 122

Query: 122 S-GGVFDGQGKEGW----------------------------RITSLDSKNSHVNILGCN 181
           S GG+FDGQGK  W                             ITSLDSK  H+N+LGC 
Sbjct: 123 SGGGIFDGQGKPTWGKDCANDEYCGDLPINIRFNYVTNSIIQEITSLDSKQFHINVLGCQ 182

Query: 182 NLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCG 241
           N+TFQ V IIA E+SP+TD IHIGRS  I +L S I  GDDCISLG+GSK++ +  VTCG
Sbjct: 183 NVTFQHVKIIAAENSPHTDGIHIGRSTDIKVLDSNIQTGDDCISLGDGSKQITIAKVTCG 242

Query: 242 PGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITM 301
           PGHGISIGSLGKY NEE V+GV VK+CT+ NT NGVRIKTW  S   G A  +HF DI M
Sbjct: 243 PGHGISIGSLGKYLNEEAVKGVDVKSCTLKNTQNGVRIKTWPDSQ-EGKASDLHFEDIIM 302

Query: 302 INVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEV 361
            NV NP++IDQEYCPWNQC  Q+PS+++I+ V FKNIRG S+T++AVKL+CS  LPC++V
Sbjct: 303 ENVGNPVMIDQEYCPWNQCVSQLPSRVQISGVRFKNIRGTSSTALAVKLVCSGGLPCQDV 362

Query: 362 KVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSIC 365
           ++ +IDL Y+G +G ITS+C NVKP +SGIQNP  C
Sbjct: 363 EMTDIDLKYSGDEGQITSECKNVKPTVSGIQNPPAC 394

BLAST of Cucsa.100530 vs. TrEMBL
Match: E3VSV7_CUCPE (Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.3e-107
Identity = 203/390 (52.05%), Postives = 259/390 (66.41%), Query Frame = 1

Query: 9   VTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASVPPSAVIIP 68
           +   L+++ + T+ + S V   F +  +G      D T+AL DAW +ACAS  PS V++P
Sbjct: 21  IPLLLLLVSNATVGATSGV---FDITTFG-AKPNADATQALADAWKEACASATPSKVLVP 80

Query: 69  KGIFKVREGEFKGPCKSPIEMRVRGTLQAL-NHPQGDSWITFVYVDRMKLSG-GVFDGQG 128
           KG +++ E   KGPCKSPIE+ V GTLQA     +GD  +   Y+D + LSG GVFDGQG
Sbjct: 81  KGTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYIDHLTLSGTGVFDGQG 140

Query: 129 KEGWR-----------------------------ITSLDSKNSHVNILGCNNLTFQGVNI 188
           K GW                              ITSLDSKN H+N+L   NLTF  + I
Sbjct: 141 KAGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKI 200

Query: 189 IAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPGHGISIGS 248
            AP +SPNTD IH+G S  + IL + IA GDDCIS+G  ++++ ++DVTCGPGHGISIGS
Sbjct: 201 TAPHNSPNTDGIHLGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGS 260

Query: 249 LGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMINVSNPILI 308
           LGKY  E+ V GV VK C ++ TTNGVRIK+W  S V+  A  MHF DI M NV+NPI+I
Sbjct: 261 LGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIII 320

Query: 309 DQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKVANIDLVY 368
           DQEYCPWNQCNR++PS IKI+KVSFKNIRG S+T VAVKL+CS + PCE V++A+IDL Y
Sbjct: 321 DQEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVCSKSNPCEGVEIADIDLTY 380

BLAST of Cucsa.100530 vs. TrEMBL
Match: W9SBW1_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_020040 PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.2e-106
Identity = 200/388 (51.55%), Postives = 263/388 (67.78%), Query Frame = 1

Query: 12  FLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASVPPSAVIIPKGI 71
           FL +LL+    + +   ++F +  YG  ++ +D+T+AL +AW DACAS     V+IP+G 
Sbjct: 12  FLSLLLAL---ASARKPLVFDVTTYGAQLN-MDITQALSNAWKDACASPIRGKVLIPRGT 71

Query: 72  FKVREGEFKGPCKSPIEMRVRGTLQALNHP----QGDSWITFVYVDRMKLSGG-VFDGQG 131
           +K+     +GPCK PI+++++GTL+A   P    +GD W+TF  VD + L+GG  FDGQG
Sbjct: 72  YKLSRATLEGPCKVPIKVQLQGTLRAPTDPSGFNEGDGWVTFRNVDGLTLAGGGTFDGQG 131

Query: 132 KEGWR----------------------------ITSLDSKNSHVNILGCNNLTFQGVNII 191
              W                             I SL+SK  H+N+LG  N+TF  V II
Sbjct: 132 SAVWGKNCEQQQYCGKLPINFRFDFVTNSLIRDIISLNSKQFHINVLGGQNVTFSRVKII 191

Query: 192 APEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPGHGISIGSL 251
           APEDSPNTD IHIGRS  ISI  + I  GDDC+SLG+G K + +  VTCGPGHGIS+GSL
Sbjct: 192 APEDSPNTDGIHIGRSSGISITDTVIQTGDDCVSLGDGCKEISIRRVTCGPGHGISVGSL 251

Query: 252 GKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMINVSNPILID 311
           GKY+NE+PVEGV+V+NCTI NT+NGVR+KTW  S   G+A  +HF DI M NV NP+LID
Sbjct: 252 GKYSNEDPVEGVIVRNCTIRNTSNGVRVKTWPDSH-DGLASNLHFEDIVMENVGNPVLID 311

Query: 312 QEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKVANIDLVYN 367
           QEYCPWNQCN ++PS+IKI+ VSFKNIRG S T++AV L+CSS  PC+ V++A+IDL Y+
Sbjct: 312 QEYCPWNQCNAKVPSRIKISDVSFKNIRGTSTTALAVNLVCSSGFPCQNVEIADIDLQYS 371

BLAST of Cucsa.100530 vs. TrEMBL
Match: A0A0D2NBK5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G193800 PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.2e-106
Identity = 199/398 (50.00%), Postives = 261/398 (65.58%), Query Frame = 1

Query: 6   SLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSG-VDVTEALKDAWNDACASVPPSA 65
           ++++T  L++     I   + +DV+    K+G +  G  D++  L +AW +ACAS  P  
Sbjct: 2   NIAITALLLLFTLIPIVECTDIDVV---AKFGAVADGQTDLSLPLMNAWKEACASPEPVN 61

Query: 66  VIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHP---QGDSWITFVYVDRMKL-SGG 125
           ++IP+G + + E    GPC++PI ++++GTL A   P   +  +W++  YVD + +  GG
Sbjct: 62  IVIPEGTYLLSEATLNGPCQAPISLQLQGTLMAPEDPTVFKEPTWVSISYVDSLTMFGGG 121

Query: 126 VFDGQGKEGW-----------------------------RITSLDSKNSHVNILGCNNLT 185
           VFDGQG   W                              IT+ DSK  HVN+LGC+N+T
Sbjct: 122 VFDGQGATAWGQNDCSKNKNCVKLPINIRFHNVKNSLIQDITTKDSKQFHVNVLGCSNIT 181

Query: 186 FQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPGH 245
           FQ   + APE+S NTD IHIGRS  + IL S+I  GDDC+SLG+GS  VKV  VTCGPGH
Sbjct: 182 FQSFTVSAPEESLNTDGIHIGRSDGVYILDSKIETGDDCVSLGDGSNNVKVQGVTCGPGH 241

Query: 246 GISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMINV 305
           GISIGSLGKY NEEP+ GV V  CT+ NT NGVRIKTW AS   G A  +HF DITM NV
Sbjct: 242 GISIGSLGKYKNEEPISGVFVTQCTLANTMNGVRIKTWPASQ-PGSATDIHFEDITMDNV 301

Query: 306 SNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKVA 365
            NPIL+DQEYCPWNQC+  +PS+++++K+SFKNIRG S T +AVKLICSS LPC+EV++A
Sbjct: 302 GNPILVDQEYCPWNQCDLSVPSRVQLSKLSFKNIRGTSKTQIAVKLICSSGLPCDEVELA 361

Query: 366 NIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSSPYT 370
           +IDL YNG +G   SQC NV P LSG+QNP+ CSS  T
Sbjct: 362 DIDLTYNGPEGPAISQCSNVHPALSGVQNPAACSSQPT 395

BLAST of Cucsa.100530 vs. TAIR10
Match: AT3G07820.1 (AT3G07820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 357.8 bits (917), Expect = 7.9e-99
Identity = 178/359 (49.58%), Postives = 234/359 (65.18%), Query Frame = 1

Query: 41  SGVDVTEALKDAWNDACASVPPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQA-LN 100
           SG D+T+AL  A+  AC S  PS V+IPKG FK+ E E +GPCK+PIE+ ++GT++A  N
Sbjct: 31  SGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGN 90

Query: 101 HPQG-DSWITFVYVDRMKLS-GGVFDGQGKEGWR-------------------------- 160
             QG + W+ F  +D  KL+ GG FDG+G   WR                          
Sbjct: 91  AIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSE 150

Query: 161 ---ITSLDSKNSHVNILGCNNLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGD 220
              I+S+D+KN H+N+LG  N+T   + I+APEDSPNTD IH+GRS  + IL S I+ GD
Sbjct: 151 IRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGD 210

Query: 221 DCISLGNGSKRVKVTDVTCGPGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKT 280
           DCIS+G+G K + V  VTCGPGHGIS+GSLG+Y +E+ V G+ V NCT+  T NG+RIKT
Sbjct: 211 DCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKT 270

Query: 281 WSASPVAGIAIIMHFSDITMINVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGC 340
           W ++  +  A  +HF DI + +VSNPILIDQEYCPWNQCN+Q  S IK+  +SFKNIRG 
Sbjct: 271 WPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGT 330

Query: 341 SATSVAVKLICSSNLPCEEVKVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSSP 368
           S    AVKL+CS   PC+ V++ +ID+ YNG  G  T  C NV P + G Q+P  CS+P
Sbjct: 331 SGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQSPKACSAP 389

BLAST of Cucsa.100530 vs. TAIR10
Match: AT3G07840.1 (AT3G07840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 345.1 bits (884), Expect = 5.3e-95
Identity = 180/398 (45.23%), Postives = 243/398 (61.06%), Query Frame = 1

Query: 1   MIKYSSLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASV 60
           M  Y  +S   F++ LL F+  +      +F++        G D+T+AL  A+  AC + 
Sbjct: 1   MGSYLGISAVLFVISLLGFSANAE-----VFNVGGS----PGSDITQALLKAFTSACQAP 60

Query: 61  PPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHP-QG-DSWITFVYVDRMKLS 120
             S V+I KG FK+ E E  GPCK+P+E+ ++GTL+A     QG + W+ F+ ++  KL+
Sbjct: 61  TASKVVITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLN 120

Query: 121 -GGVFDGQGKEGWRITSLD-----------------------------SKNSHVNILGCN 180
            GG+FDG+G   WR+ +                               +KN H+N+LG  
Sbjct: 121 GGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAK 180

Query: 181 NLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCG 240
           N+TF  V +IAP +SPNTD IH+GRS  + IL S+IA GDDCIS+G+G K + V +V CG
Sbjct: 181 NMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCG 240

Query: 241 PGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITM 300
           PGHGIS+GSLG+Y +E+ V G+ V NCT+  T NG+RIKTW ++  A  A  +HF +I +
Sbjct: 241 PGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIIL 300

Query: 301 INVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEV 360
            NVSNPILIDQEYCPWNQCN+Q PS IK+  +SFKNIRG S    AVKL+CS   PC  V
Sbjct: 301 NNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANV 360

Query: 361 KVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSS 367
           ++ NI+L Y G  G  T  C NV P L G QNP  CS+
Sbjct: 361 EIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPKACSA 389

BLAST of Cucsa.100530 vs. TAIR10
Match: AT5G48140.1 (AT5G48140.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 342.0 bits (876), Expect = 4.5e-94
Identity = 171/363 (47.11%), Postives = 228/363 (62.81%), Query Frame = 1

Query: 38  NIVSGVDVTEALKDAWNDACASVPPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQA 97
           N   G D+T AL  A+N+AC     S V+IPKG +K+ E    GPCK+PI + + GT++A
Sbjct: 29  NSPPGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKA 88

Query: 98  LNHPQG-DSWITFVYVDRMKLS-GGVFDGQGKEGWRITSL-------------------- 157
             +  G + W+ F  ++  KL+ GGVFDG+G   WR+ +                     
Sbjct: 89  DGNANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTD 148

Query: 158 ---------DSKNSHVNILGCNNLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAI 217
                    D+K+ H+N++G  N+TF+ V IIAP +SPNTD IH+GRS  I I+ S I+ 
Sbjct: 149 AKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFIST 208

Query: 218 GDDCISLGNGSKRVKVTDVTCGPGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRI 277
           GDDC+S+G+G K + V  VTCGPGHGISIGSLG+Y++EE V G+ + NCT+  T NG+RI
Sbjct: 209 GDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRI 268

Query: 278 KTWSASPVAGIAIIMHFSDITMINVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIR 337
           KTW ++     A  +HF +I + NVSNPILIDQEYCPWNQCN+Q PS IK+  +SFK IR
Sbjct: 269 KTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIR 328

Query: 338 GCSATSVAVKLICSSNLPCEEVKVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSS 370
           G S    AVKL+CS   PC+ V+V ++++ Y G  G  T QC NV P L G Q P  CSS
Sbjct: 329 GTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKACSS 388

BLAST of Cucsa.100530 vs. TAIR10
Match: AT3G14040.1 (AT3G14040.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 311.6 bits (797), Expect = 6.5e-85
Identity = 163/358 (45.53%), Postives = 220/358 (61.45%), Query Frame = 1

Query: 44  DVTEALKDAWNDACASVPPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQA-----L 103
           D + A   AW +ACA+   S + +PKG + V   EFKGPCK P+ + + G  +A      
Sbjct: 85  DDSAAFAAAWKEACAA--GSTITVPKGEYLVESLEFKGPCKGPVTLELNGNFKAPATVKT 144

Query: 104 NHPQGDSWITFVYVDRMKLSG--GVFDGQGKEGWR------------------------- 163
             P    WI F  +    L+G   +FDGQG   W+                         
Sbjct: 145 TKPHA-GWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNS 204

Query: 164 ----ITSLDSKNSHVNILGCNNLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIG 223
               ITS +SK  H+NIL C N+T   + I AP +S NTD IHIGRS  ++++ ++I  G
Sbjct: 205 KINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTG 264

Query: 224 DDCISLGNGSKRVKVTDVTCGPGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIK 283
           DDC+S+G+G++ + V +V CGPGHGISIGSLG+Y NE+PV+GV V+ C I NT NGVRIK
Sbjct: 265 DDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIK 324

Query: 284 TWSASPVAGIAIIMHFSDITMINVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRG 343
           TW  SP  GIA  + F DITM NVS P+LIDQEYCP+  C   +PSK+K++ V+ KNI+G
Sbjct: 325 TWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKG 384

Query: 344 CSATSVAVKLICSSNLPCEEVKVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICS 366
            SAT VAVKL+CS  +PC  + +++I+LV+NG +G   S C N+KPILSG   P+ C+
Sbjct: 385 TSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAACT 438

BLAST of Cucsa.100530 vs. TAIR10
Match: AT3G07850.1 (AT3G07850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 310.1 bits (793), Expect = 1.9e-84
Identity = 162/361 (44.88%), Postives = 220/361 (60.94%), Query Frame = 1

Query: 41  SGVDVTEALKDAWNDACASVPPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQA--- 100
           S  D + A   AW +ACA+   S + +PKG + V   EFKGPCK P+ + + G  +A   
Sbjct: 81  SKTDDSAAFAAAWKEACAA--GSTITVPKGEYMVESLEFKGPCKGPVTLELNGNFKAPAT 140

Query: 101 --LNHPQGDSWITFVYVDRMKLSG--GVFDGQGKEGWR---------------------- 160
                P    WI F  +    L+G   +FDGQG   W+                      
Sbjct: 141 VKTTKPHA-GWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGL 200

Query: 161 -------ITSLDSKNSHVNILGCNNLTFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRI 220
                  ITS +SK  H+NIL C N+T   + I AP +S NTD IHIGRS  ++++ ++I
Sbjct: 201 TNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKI 260

Query: 221 AIGDDCISLGNGSKRVKVTDVTCGPGHGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGV 280
             GDDC+S+G+G++ + V +V CGPGHGISIGSLG+Y NE+PV+GV V+ C I NT NGV
Sbjct: 261 KTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGV 320

Query: 281 RIKTWSASPVAGIAIIMHFSDITMINVSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKN 340
           RIKTW  SP  GIA  + F DITM NVS P+LIDQEYCP+  C   +PS++K++ V+ K 
Sbjct: 321 RIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIKG 380

Query: 341 IRGCSATSVAVKLICSSNLPCEEVKVANIDLVYNGTKGSITSQCMNVKPILSGIQNPSIC 366
           I+G SAT VAVKL+CS  +PC  + +++I+LV+NG +G   S C N+KPILSG   P+ C
Sbjct: 381 IKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAAC 437

BLAST of Cucsa.100530 vs. NCBI nr
Match: gi|659111053|ref|XP_008455553.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 651.7 bits (1680), Expect = 7.5e-184
Identity = 327/399 (81.95%), Postives = 342/399 (85.71%), Query Frame = 1

Query: 1   MIKYSSLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASV 60
           +IKYSSL V TFLM LLSFT+ESRSHVDVIFHLRKYGNIVSG DVTEALKDAWNDACAS+
Sbjct: 3   VIKYSSLLVITFLMALLSFTVESRSHVDVIFHLRKYGNIVSGADVTEALKDAWNDACASL 62

Query: 61  PPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHPQGDSWITFVYVDRMKLSGG 120
            PSAV+IPKG FKVREGEFKGPCKSP+E+RVRGTLQA  HP GDSWITF YVDRM LSGG
Sbjct: 63  RPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWITFAYVDRMTLSGG 122

Query: 121 -VFDGQGKEGW-----------------------------RITSLDSKNSHVNILGCNNL 180
            VFDGQGK GW                             RITSLDSKN HVNILGCNNL
Sbjct: 123 GVFDGQGKAGWEKNDCHKHISCARLPISLRFNFITNSIVRRITSLDSKNFHVNILGCNNL 182

Query: 181 TFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPG 240
           TF GVNIIAPEDSPNTD IHIGRSI ISILKSRIA GDDCISLG+GSKRVK+T+VTCGPG
Sbjct: 183 TFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRVKITNVTCGPG 242

Query: 241 HGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMIN 300
           HGISIGSLGKYTNEEPVEGV+VKNCTIMNTTNGVRIKTW ASPVAGIA  MHF DITM+N
Sbjct: 243 HGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVAGIATNMHFLDITMVN 302

Query: 301 VSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKV 360
           VSNPILIDQEYCPWNQCNR+IPSKIKINKVSFKNIRG SAT VAVKLICSSNLPCEEVKV
Sbjct: 303 VSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKV 362

Query: 361 ANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSSPYT 370
           ANIDLVYNG KGS+TS+CMNVKPI+SG QNP ICSS YT
Sbjct: 363 ANIDLVYNGIKGSLTSECMNVKPIISGTQNPPICSSSYT 401

BLAST of Cucsa.100530 vs. NCBI nr
Match: gi|700188231|gb|KGN43464.1| (hypothetical protein Csa_7G037910 [Cucumis sativus])

HSP 1 Score: 632.9 bits (1631), Expect = 3.6e-178
Identity = 323/403 (80.15%), Postives = 338/403 (83.87%), Query Frame = 1

Query: 1   MIKYSSLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASV 60
           +IK+  L VTTFLM LLSFTIE+RSHVD IF LRKYGNIVSG DVTEALKDAWNDACAS 
Sbjct: 3   VIKHLILLVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASD 62

Query: 61  PPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHPQGDSWITFVYVDRMKLSGG 120
            PSAV+IPKG FKVREGEFKGPCKSPIE RV GTLQA  HP GDSWITF YVDRM+LSGG
Sbjct: 63  RPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGG 122

Query: 121 -VFDGQGKEGW-----------------------------RITSLDSKNSHVNILGCNNL 180
            VFDGQGK GW                             RITSLDSKN HVNILGCNNL
Sbjct: 123 GVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNFHVNILGCNNL 182

Query: 181 TFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPG 240
           TFQGVNIIAPE+SPNTD IHIGRSI ISILKSRIA GDDCISLG+GSKR+KV +V CGPG
Sbjct: 183 TFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRIKVNNVICGPG 242

Query: 241 HGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMIN 300
           HGISIGSLGKY NEEPVEGV+V NCTI+NTTNGVRIKTW ASPVAGIA  MHFSDITM+N
Sbjct: 243 HGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATNMHFSDITMVN 302

Query: 301 VSNPILIDQEYCPWNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKV 360
           VSNPILIDQEYCPWNQCNR+IPS IKINKVSFKNIRG SAT VAVKLICSSNLPCEEVKV
Sbjct: 303 VSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKV 362

Query: 361 ANIDLVYNGTKGSITSQCMNVKPILSGIQNPSICSSPYTIASK 374
           ANIDLVYNG KGSITS+CMNVKPI+SGIQNP ICSS YT ASK
Sbjct: 363 ANIDLVYNGIKGSITSECMNVKPIISGIQNPPICSSSYTTASK 404

BLAST of Cucsa.100530 vs. NCBI nr
Match: gi|778730103|ref|XP_011659699.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 619.8 bits (1597), Expect = 3.2e-174
Identity = 315/390 (80.77%), Postives = 328/390 (84.10%), Query Frame = 1

Query: 14  MILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASVPPSAVIIPKGIFK 73
           M LLSFTIE+RSHVD IF LRKYGNIVSG DVTEALKDAWNDACAS  PSAV+IPKG FK
Sbjct: 1   MSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVIPKGTFK 60

Query: 74  VREGEFKGPCKSPIEMRVRGTLQALNHPQGDSWITFVYVDRMKLSGG-VFDGQGKEGW-- 133
           VREGEFKGPCKSPIE RV GTLQA  HP GDSWITF YVDRM+LSGG VFDGQGK GW  
Sbjct: 61  VREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGGGVFDGQGKAGWEK 120

Query: 134 ---------------------------RITSLDSKNSHVNILGCNNLTFQGVNIIAPEDS 193
                                      RITSLDSKN HVNILGCNNLTFQGVNIIAPE+S
Sbjct: 121 NDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNFHVNILGCNNLTFQGVNIIAPENS 180

Query: 194 PNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPGHGISIGSLGKYTN 253
           PNTD IHIGRSI ISILKSRIA GDDCISLG+GSKR+KV +V CGPGHGISIGSLGKY N
Sbjct: 181 PNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNN 240

Query: 254 EEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMINVSNPILIDQEYCP 313
           EEPVEGV+V NCTI+NTTNGVRIKTW ASPVAGIA  MHFSDITM+NVSNPILIDQEYCP
Sbjct: 241 EEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCP 300

Query: 314 WNQCNRQIPSKIKINKVSFKNIRGCSATSVAVKLICSSNLPCEEVKVANIDLVYNGTKGS 373
           WNQCNR+IPS IKINKVSFKNIRG SAT VAVKLICSSNLPCEEVKVANIDLVYNG KGS
Sbjct: 301 WNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGS 360

BLAST of Cucsa.100530 vs. NCBI nr
Match: gi|778730075|ref|XP_011659692.1| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 482.6 bits (1241), Expect = 6.0e-133
Identity = 247/321 (76.95%), Postives = 261/321 (81.31%), Query Frame = 1

Query: 1   MIKYSSLSVTTFLMILLSFTIESRSHVDVIFHLRKYGNIVSGVDVTEALKDAWNDACASV 60
           MIKYS+LS+TTFLM LLSFTIES+SHVDVIF LRKYGNI        ALKDAWNDACASV
Sbjct: 3   MIKYSNLSITTFLMSLLSFTIESQSHVDVIFPLRKYGNI--------ALKDAWNDACASV 62

Query: 61  PPSAVIIPKGIFKVREGEFKGPCKSPIEMRVRGTLQALNHPQGDSWITFVYVDRMKLSG- 120
            PSAV+I  GIFKVREGEFKGPCKSPIE+RVRGTLQA  HP GDSWITF YVDRM +SG 
Sbjct: 63  RPSAVVIRDGIFKVREGEFKGPCKSPIEIRVRGTLQAPKHPHGDSWITFAYVDRMTISGK 122

Query: 121 GVFDGQGKEGWR-----------------------------ITSLDSKNSHVNILGCNNL 180
           GVFDGQGK GW                              ITSLDSKN HVNILGCNNL
Sbjct: 123 GVFDGQGKAGWEKNDCHKRIDCVNLPISLRFDFITNSIVRGITSLDSKNFHVNILGCNNL 182

Query: 181 TFQGVNIIAPEDSPNTDEIHIGRSIRISILKSRIAIGDDCISLGNGSKRVKVTDVTCGPG 240
           TFQGVNIIAPEDSPNTD IHIGRSI ISILKSRIA GDDCISLG+GSK+VKV +VTCGPG
Sbjct: 183 TFQGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKQVKVANVTCGPG 242

Query: 241 HGISIGSLGKYTNEEPVEGVVVKNCTIMNTTNGVRIKTWSASPVAGIAIIMHFSDITMIN 292
           HGISIGSLGKYTNEEPV+ V VKNCTIMNTTNGVRIKTW +SPVAGIA  MHFSDITM+N
Sbjct: 243 HGISIGSLGKYTNEEPVDDVEVKNCTIMNTTNGVRIKTWPSSPVAGIATNMHFSDITMVN 302

BLAST of Cucsa.100530 vs. NCBI nr
Match: gi|659119537|ref|XP_008459709.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 433.3 bits (1113), Expect = 4.2e-118
Identity = 212/370 (57.30%), Postives = 265/370 (71.62%), Query Frame = 1

Query: 30  IFHLRKYGNIVSGVDVTEALKDAWNDACASVPPSAVIIPKGIFKVREGEFKGPCKSPIEM 89
           IF + KYG      D+T+AL DAW +ACAS  P  ++IP G +K+   E KG CKSPIE+
Sbjct: 54  IFDVTKYG-AKPNADITQALADAWKEACASTSPCKIVIPTGTYKLGTIELKGECKSPIEI 113

Query: 90  RVRGTLQALNHPQGDSWITFVYVDRMKL-SGGVFDGQGKEGWR----------------- 149
           +V+GTLQA     G+ W+ F Y+D + + SGGVFDGQGK+ W                  
Sbjct: 114 QVQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFDGQGKQAWESNDCDKNPKCARIPMNL 173

Query: 150 ------------ITSLDSKNSHVNILGCNNLTFQGVNIIAPEDSPNTDEIHIGRSIRISI 209
                       ITS DSKN H+ +LGCNNLTFQ VN+ APE S NTD IHIGRS+ I+I
Sbjct: 174 KFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINTDGIHIGRSLGINI 233

Query: 210 LKSRIAIGDDCISLGNGSKRVKVTDVTCGPGHGISIGSLGKYTNEEPVEGVVVKNCTIMN 269
           L + I  GDDCISLG+GSK+V +T+VTCGPGHGIS+GSLG+Y  EEPVEG+ VKNCT+  
Sbjct: 234 LNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQVKNCTLTG 293

Query: 270 TTNGVRIKTWSASPVAGIAIIMHFSDITMINVSNPILIDQEYCPWNQCNRQIPSKIKINK 329
           TTNG+RIKTW ASP AG+A  MH+ D+ M NV NPILIDQEYCP+NQCN+Q+PSK+KI+K
Sbjct: 294 TTNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVPSKVKISK 353

Query: 330 VSFKNIRGCSATSVAVKLICSSNLPCEEVKVANIDLVYNGTKGSITSQCMNVKPILSGIQ 370
           VSFKNIRG S  + AV L+CSS+ PC+ V++A+IDL Y+G++G ITS+C NVKP +SG Q
Sbjct: 354 VSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVKPTVSGKQ 413