BLAST of ClCG03G008430 vs. Swiss-Prot
Match:
CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)
HSP 1 Score: 226.5 bits (576), Expect = 5.2e-58
Identity = 118/248 (47.58%), Postives = 162/248 (65.32%), Query Frame = 1
Query: 154 NFTRQEGKINPTAL---FLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMG 213
N T +GK N TA+ F S+++G V+ LV ++N +FPELG+ K C + SW++ T+I
Sbjct: 296 NITDNQGK-NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWID-TIIFY 355
Query: 214 TGL----QPGKALEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQL 273
+G+ E LL +++ N + KIK DY+K+PIP + I ++L EDI
Sbjct: 356 SGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMY 415
Query: 274 LFSPYGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPF 333
PYGGIM +ISES PF HR G LY + Y+ +WE+Q E +KHL+WIR +Y++MTP+
Sbjct: 416 ALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPY 475
Query: 334 ASKSPRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFK 393
SK+PR AY+NY+DLDIG N+ +Y QA IWG KYFG NF+RLV +KT VDP +FF+
Sbjct: 476 VSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFR 535
Query: 394 HEQSIPTL 395
+EQSIP L
Sbjct: 536 NEQSIPPL 539
HSP 2 Score: 161.8 bits (408), Expect = 1.6e-38
Identity = 75/134 (55.97%), Postives = 99/134 (73.88%), Query Frame = 1
Query: 22 VASNSSEKHENFLQCLSHHSPNISN---VIYTPFNSSYSSILNFTLRNLRFSTPNTPKPL 81
+ ++ + ENFL+C S + PN + ++YT N Y S+LN T+ NLRF++ TPKPL
Sbjct: 23 IQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPL 82
Query: 82 LIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLPFIIVDLINLNSISIEV 141
+I+TPSH+SHIQ ++CSK GLQIRTRSGGHD EG+SY+S +PF+IVDL N+ SI I+V
Sbjct: 83 VIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDV 142
Query: 142 ETNTAWVESGATLG 153
+ TAWVE+GATLG
Sbjct: 143 HSQTAWVEAGATLG 156
BLAST of ClCG03G008430 vs. Swiss-Prot
Match:
CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)
HSP 1 Score: 224.6 bits (571), Expect = 2.0e-57
Identity = 113/247 (45.75%), Postives = 160/247 (64.78%), Query Frame = 1
Query: 154 NFTRQEGKINPT--ALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGT 213
N T +GK T + F S++ G V+ LV ++N +FPELG+ K C + SW++ T+I +
Sbjct: 297 NITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWID-TIIFYS 356
Query: 214 GLQPGKAL----EDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLL 273
G+ E LL ++ A+ IK DY+K+PIP A+ I ++L ED+ + +
Sbjct: 357 GVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFV 416
Query: 274 FSPYGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFA 333
F PYGGIM +ISES PF HR G +Y I Y+ +WE+Q E +KH++WIR +Y++ TP+
Sbjct: 417 FYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYV 476
Query: 334 SKSPRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKH 393
S++PR AY+NY+DLD+G N +Y QA IWG KYFG NFNRLV +KT+VDP +FF++
Sbjct: 477 SQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRN 536
Query: 394 EQSIPTL 395
EQSIP L
Sbjct: 537 EQSIPPL 540
HSP 2 Score: 157.9 bits (398), Expect = 2.3e-37
Identity = 77/146 (52.74%), Postives = 107/146 (73.29%), Query Frame = 1
Query: 10 LVFILLSSSSPWVASNSSEKHENFLQCLSHHSP-NISN--VIYTPFNSSYSSILNFTLRN 69
++F LS + + + + ENFL+C S + P N++N ++YT + Y SILN T++N
Sbjct: 14 IIFFFLSFN---IQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQN 73
Query: 70 LRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLPFIIV 129
LRF++ TPKPL+IITP ++SHIQ ++CSK GLQIRTRSGGHD EG+SY+S +PF+IV
Sbjct: 74 LRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIV 133
Query: 130 DLINLNSISIEVETNTAWVESGATLG 153
DL N++S+ I+V + TAWVE+GATLG
Sbjct: 134 DLRNMHSVKIDVHSQTAWVEAGATLG 156
BLAST of ClCG03G008430 vs. Swiss-Prot
Match:
CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)
HSP 1 Score: 223.4 bits (568), Expect = 4.4e-57
Identity = 113/247 (45.75%), Postives = 159/247 (64.37%), Query Frame = 1
Query: 154 NFTRQEGKINPT--ALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGT 213
N T +GK T + F S++ G V+ LV ++N +FPELG+ K C + SW++ T+I +
Sbjct: 297 NITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWID-TIIFYS 356
Query: 214 GLQPGKAL----EDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLL 273
G+ E LL ++ A+ IK DY+K+PIP A+ I ++L ED+ + +
Sbjct: 357 GVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFV 416
Query: 274 FSPYGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFA 333
F PYGGIM +ISES PF HR G Y I Y+ +WE+Q E +KH++WIR +Y++ TP+
Sbjct: 417 FYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYV 476
Query: 334 SKSPRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKH 393
S++PR AY+NY+DLD+G N +Y QA IWG KYFG NFNRLV +KT+VDP +FF++
Sbjct: 477 SQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRN 536
Query: 394 EQSIPTL 395
EQSIP L
Sbjct: 537 EQSIPPL 540
HSP 2 Score: 158.3 bits (399), Expect = 1.8e-37
Identity = 78/146 (53.42%), Postives = 107/146 (73.29%), Query Frame = 1
Query: 10 LVFILLSSSSPWVASNSSEKHENFLQCLSHHSP-NISN--VIYTPFNSSYSSILNFTLRN 69
++F LS + + + + ENFL+CLS + P N++N ++YT + Y SILN T++N
Sbjct: 14 IIFFFLSFN---IQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQN 73
Query: 70 LRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLPFIIV 129
LRF++ TPKPL+I TP ++SHIQ ++CSK GLQIRTRSGGHD EG+SY+S +PF+IV
Sbjct: 74 LRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIV 133
Query: 130 DLINLNSISIEVETNTAWVESGATLG 153
DL N++S+ I+V + TAWVESGATLG
Sbjct: 134 DLRNMHSVKIDVHSQTAWVESGATLG 156
BLAST of ClCG03G008430 vs. Swiss-Prot
Match:
THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)
HSP 1 Score: 220.7 bits (561), Expect = 2.9e-56
Identity = 109/246 (44.31%), Postives = 154/246 (62.60%), Query Frame = 1
Query: 154 NFTRQEGKINPTA--LFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGT 213
N T GK T F S++ G V+ LV ++N +FPELG+ K C E SW+++T+
Sbjct: 297 NITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSG 356
Query: 214 GLQPGKA---LEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLF 273
+ A E LL +++ + IK DY+K+PIP A+ I ++L ED+ +
Sbjct: 357 VVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVL 416
Query: 274 SPYGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFAS 333
PYGGIM +ISES PF HR G +Y + Y +WE+Q E +KH++W+R +Y++ TP+ S
Sbjct: 417 YPYGGIMEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVS 476
Query: 334 KSPRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHE 393
++PR AY+NY+DLD+G N +Y QA IWG KYFG NFNRLV +KT+VDP +FF++E
Sbjct: 477 QNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNE 536
Query: 394 QSIPTL 395
QSIP L
Sbjct: 537 QSIPPL 540
HSP 2 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 78/146 (53.42%), Postives = 105/146 (71.92%), Query Frame = 1
Query: 10 LVFILLSSSSPWVASNSSEKHENFLQCLSHHSPN-ISN--VIYTPFNSSYSSILNFTLRN 69
++F LS + + + ENFL+C S H PN ++N ++YT + Y SILN T++N
Sbjct: 14 IIFFFLSFH---IQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQN 73
Query: 70 LRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLPFIIV 129
LRF + TPKPL+I+TPS+ SHIQ ++CSK GLQIRTRSGGHD EG+SY+S +PF++V
Sbjct: 74 LRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVV 133
Query: 130 DLINLNSISIEVETNTAWVESGATLG 153
DL N++SI I+V + TAWVE+GATLG
Sbjct: 134 DLRNMHSIKIDVHSQTAWVEAGATLG 156
BLAST of ClCG03G008430 vs. Swiss-Prot
Match:
THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)
HSP 1 Score: 216.1 bits (549), Expect = 7.1e-55
Identity = 106/246 (43.09%), Postives = 153/246 (62.20%), Query Frame = 1
Query: 154 NFTRQEGKINPTA--LFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGT 213
N T GK T F S+++G V+ LV ++N +FPELG+ K C E SW+++T+
Sbjct: 297 NITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSG 356
Query: 214 GLQPGKA---LEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLF 273
+ A E LL +++ + IK DY+K+ IP A+ I ++L E++ + +
Sbjct: 357 VVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVL 416
Query: 274 SPYGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFAS 333
PYGGIM +ISES PF HR G +Y + Y WE+Q E +KH++W+R +Y++ TP+ S
Sbjct: 417 YPYGGIMDEISESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVS 476
Query: 334 KSPRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHE 393
++PR AY+NY+DLD+G N +Y QA IWG KYFG NFNRLV +KT+ DP +FF++E
Sbjct: 477 QNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNE 536
Query: 394 QSIPTL 395
QSIP L
Sbjct: 537 QSIPPL 540
HSP 2 Score: 156.8 bits (395), Expect = 5.1e-37
Identity = 74/146 (50.68%), Postives = 104/146 (71.23%), Query Frame = 1
Query: 10 LVFILLSSSSPWVASNSSEKHENFLQCLSHHSPNIS---NVIYTPFNSSYSSILNFTLRN 69
++F LS + + + + ENFL+C S + PN IYT + Y S+LN T++N
Sbjct: 14 IIFFFLSFN---IQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQN 73
Query: 70 LRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLPFIIV 129
LRF++ TPKPL+I+TPS++SHIQ +++CSK GLQIRTRSGGHD EGLSY+S +PF IV
Sbjct: 74 LRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIV 133
Query: 130 DLINLNSISIEVETNTAWVESGATLG 153
DL N++++ +++ + TAWVE+GATLG
Sbjct: 134 DLRNMHTVKVDIHSQTAWVEAGATLG 156
BLAST of ClCG03G008430 vs. TrEMBL
Match:
A0A067KWX9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09769 PE=4 SV=1)
HSP 1 Score: 349.4 bits (895), Expect = 6.0e-93
Identity = 188/409 (45.97%), Postives = 261/409 (63.81%), Query Frame = 1
Query: 8 LSLVFILLSSSSPWVASNSSEKHENFLQCL---SHHSPNISNVIYTPFNSSYSSILNFTL 67
L +F + S WV + S+ HE+FL+CL S S +IS VIYTP NSSY S+L +
Sbjct: 7 LPFLFAVFLFSFSWVTTTSAHTHEDFLECLKLQSEDSSSISKVIYTPINSSYLSVLQLGI 66
Query: 68 RNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLPFI 127
N RF+T TPKP +I+TP ++SHI+ + CSK + LQIR RSGGHD+EGLSYVS PF+
Sbjct: 67 NNERFNTSTTPKPQVIVTPLNLSHIEATITCSKKHDLQIRIRSGGHDYEGLSYVSPNPFV 126
Query: 128 IVDLINLNSISIEVETNTAWVESGATLGGNF----TRQEGKINPTALFLSLYIG------ 187
I+DLI L SI+++ TAWV+ GAT G + + + P ++ ++ IG
Sbjct: 127 ILDLITLQSITVDATNKTAWVQVGATTGKLYYSIAEKSQNLAFPASVCTTVGIGGHFSGG 186
Query: 188 ------KVEELV--SILNTTFPEL-GLAKKHCIETSWVESTLIMGTGLQPGKALEDLLSK 247
+ L +I++ ++ GL K+ E SW+ES L G +LE LL +
Sbjct: 187 GYGTLLRKYGLAADNIIDAQLTDVDGLTKEDSTEMSWIESVLYF-AGFS-NSSLEILLER 246
Query: 248 ASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQISESETPF 307
K KSDY+++P+P A+EGIW+RL ++ QL+F+ YGG +S+ISES PF
Sbjct: 247 TQQNVRYFKAKSDYVQQPMPEVALEGIWKRLFEVEVGSGQLMFNLYGGRVSEISESSIPF 306
Query: 308 SHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNYKDLDIGS 367
HR G LY I + + W +G E H+SWIR LYS++ P+ SK+PR+AY+NY+DLDIG
Sbjct: 307 PHRAGNLYKIQHSVYWNDEGIEESITHISWIRRLYSFLAPYVSKNPRSAYINYRDLDIGI 366
Query: 368 NNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTL 395
NN G TSY+QASIWG+KYF NF+RLV +KT+VDP +FF++EQSIP+L
Sbjct: 367 NN-IGNTSYEQASIWGIKYFKINFDRLVHVKTQVDPANFFRNEQSIPSL 412
BLAST of ClCG03G008430 vs. TrEMBL
Match:
A0A0A0LY37_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G539350 PE=4 SV=1)
HSP 1 Score: 331.6 bits (849), Expect = 1.3e-87
Identity = 155/250 (62.00%), Postives = 193/250 (77.20%), Query Frame = 1
Query: 151 LGGNFTRQEGKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMG 210
+GG + Q KINP ALF SL++GK +EL++ILN TFP+LGL K+ C ETSW+ES + G
Sbjct: 7 IGGKISAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTG 66
Query: 211 TGLQ-PGKALEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFS 270
GLQ + LE LL++ L + K+KSDY+K PIP A +E IWQRL+ +DIE L+F
Sbjct: 67 NGLQIEDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFV 126
Query: 271 PYGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASK 330
PYGG MSQIS+SE PFSHR G LY IGY+ W + G AEK HL+WIR++Y YMTPF SK
Sbjct: 127 PYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSK 186
Query: 331 SPRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQ 390
SPRAAYVNY+DLDIGSN++YGKTSYK+A +WGLKYFGNNFNR+V +K +VDPY+FF+HEQ
Sbjct: 187 SPRAAYVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQ 246
Query: 391 SIPTLSNKKV 400
SIP L K+
Sbjct: 247 SIPVLLKHKI 256
BLAST of ClCG03G008430 vs. TrEMBL
Match:
A0A151RZF5_CAJCA (Reticuline oxidase-like protein OS=Cajanus cajan GN=KK1_030435 PE=4 SV=1)
HSP 1 Score: 328.2 bits (840), Expect = 1.4e-86
Identity = 176/401 (43.89%), Postives = 252/401 (62.84%), Query Frame = 1
Query: 6 IFLSLVFILLSSSSPWVASNSSEKHENFLQCL---SHHSPNISNVIYTPFNSSYSSILNF 65
++ ++V I L S A ++ + H+NF+ CL H++ +IS +YT NSSYS L+F
Sbjct: 6 LYFTVVAIALLFS---FAYSTPDTHKNFVGCLYNYPHNTTSISKAVYTQTNSSYSFTLDF 65
Query: 66 TLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLP 125
++RNLRF ++ KPL+I+TP +SHIQ +ICS+ +G+QIRTRSGGHD+EGLSYV+ +P
Sbjct: 66 SIRNLRFYNVSS-KPLVIVTPLDVSHIQATIICSQRHGMQIRTRSGGHDYEGLSYVAEVP 125
Query: 126 FIIVDLINLNSISIEVETNTAWVESGATLGGNFTRQEGKINPTALFLSLYIGKVEELVSI 185
F++VDLINL I+++VE +TAWV++GAT+G EL
Sbjct: 126 FVVVDLINLREINVDVENSTAWVQAGATIG-------------------------ELYYS 185
Query: 186 LNTTFPELGLAKKHCIETSWVESTLIMGTGL---QPGKALEDLLS------KASLTNAST 245
++ LG C G G + G A ++++ K ++ + +
Sbjct: 186 ISQKSKTLGFPAGMCPPVGTGGHFSGGGYGFLMRRYGLAADNIIDAHIIDVKGNVLDRAG 245
Query: 246 KIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQISESETPFSHRYGYLY 305
KSDY++ PIP ++ +W ++ + + F+PYGG M +ISESE PF HR G ++
Sbjct: 246 MAKSDYVRDPIPDVGLQCLWPFFYEDEAQAAYVQFTPYGGRMDEISESEIPFPHRSGNIF 305
Query: 306 SIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNYKDLDIGSNNRYGKTS 365
I Y + W+++G EA ++H+ WIR +YSYM + SKSPRAAYVNY+DLDIG NN G TS
Sbjct: 306 HIQYGVEWQEEGEEAAQRHVEWIRRVYSYMEAYVSKSPRAAYVNYRDLDIGVNND-GYTS 365
Query: 366 YKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTL 395
Y QASIWGLKYF NNFNRL +KTRVDP +FF++EQSIPT+
Sbjct: 366 YSQASIWGLKYFKNNFNRLARVKTRVDPLNFFRNEQSIPTI 376
BLAST of ClCG03G008430 vs. TrEMBL
Match:
A0A0A0LVX6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G539340 PE=4 SV=1)
HSP 1 Score: 324.3 bits (830), Expect = 2.1e-85
Identity = 156/228 (68.42%), Postives = 186/228 (81.58%), Query Frame = 1
Query: 168 FLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGTGL-QPGKALEDLLSKA 227
FL +Y ELV+ L+T FPELGL K+ C+E WVESTLI+ +G+ Q ++LE LL++
Sbjct: 11 FLDIY-----ELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIESLEPLLNRT 70
Query: 228 SLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQISESETPFS 287
T STKIKSDYIK+PIP AA+EGIWQRLK DIE PQ +F+PYG MSQIS+SETPFS
Sbjct: 71 PTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSETPFS 130
Query: 288 HRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNYKDLDIGSN 347
HR GYLY IGY++ W+ Q +A+ +H+SWIRELY YM PF SKSPRAAY NY+DLDIGSN
Sbjct: 131 HRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYTNYRDLDIGSN 190
Query: 348 NRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTL 395
N+YGKTSYKQASIWGLKYFGNNF RLV +KT+VDP+DFF+HEQSIPTL
Sbjct: 191 NKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 233
BLAST of ClCG03G008430 vs. TrEMBL
Match:
A0A0A0LYL7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G539330 PE=4 SV=1)
HSP 1 Score: 316.6 bits (810), Expect = 4.3e-83
Identity = 146/215 (67.91%), Postives = 178/215 (82.79%), Query Frame = 1
Query: 180 VSILNTTFPELGLAKKHCIETSWVESTLIMGTGLQPGKALEDLLSKASLTNASTKIKSDY 239
++ L+TTFPELGL K+ C+E SWVESTLI+ G+QP ++LE LL++ STKIKSDY
Sbjct: 1 MATLSTTFPELGLIKQDCVEASWVESTLIIPIGVQPIESLEPLLNRTPTYLDSTKIKSDY 60
Query: 240 IKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQISESETPFSHRYGYLYSIGYVI 299
+K PI A +EGIWQRLK +DIE Q++F PYGG MSQISESETPF HR GYL+ I YV+
Sbjct: 61 VKEPISEATIEGIWQRLKAQDIETSQVIFVPYGGRMSQISESETPFPHRAGYLFKIAYVV 120
Query: 300 NWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNYKDLDIGSNNRYGKTSYKQASI 359
W+ Q +A+K H+SWIRE+Y YM PF SKSPRAAY NY+DLDIGSNN+YGKTSYK+ASI
Sbjct: 121 GWKDQSLKAKKTHISWIREIYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKRASI 180
Query: 360 WGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTL 395
WG+KYFG+NF+RLV +KT+VDPYDFF+HEQSIPTL
Sbjct: 181 WGMKYFGDNFDRLVYVKTKVDPYDFFRHEQSIPTL 215
BLAST of ClCG03G008430 vs. TAIR10
Match:
AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)
HSP 1 Score: 219.2 bits (557), Expect = 4.7e-57
Identity = 108/236 (45.76%), Postives = 155/236 (65.68%), Query Frame = 1
Query: 160 GKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGTGLQPGKAL 219
G T + +L++G L++++ +FPELGL K CIE SW+ES + +G
Sbjct: 305 GNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYI-SGFPTHTPT 364
Query: 220 EDLLS-KASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQI 279
LL K+ S K KSD++K PIP + ++GI+++L EDI P ++++PYGG+M++I
Sbjct: 365 NVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKI 424
Query: 280 SESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNY 339
ES+ PF HR G L+ + YV +W ++ +H++WIR+LYSYMTP+ S +PR AYVNY
Sbjct: 425 PESQIPFPHRKGVLFKVQYVTSW-LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNY 484
Query: 340 KDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTL 395
+DLD+G N + KT KQA +WG YF NNFNRL+ IK +VDP +FF+HEQSIP +
Sbjct: 485 RDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536
HSP 2 Score: 117.5 bits (293), Expect = 1.9e-26
Identity = 69/185 (37.30%), Postives = 107/185 (57.84%), Query Frame = 1
Query: 2 KSSPIFLSLV-FILLSSSSPWVASNSSEKHENFLQCLSHHSP---NISNVIYTPFN--SS 61
K P+F LV + L + +P S+ + + F+ C+ ++ + + P S
Sbjct: 5 KPLPLFSILVLYFSLYTITP--TSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSM 64
Query: 62 YSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGL 121
+S +L T +NLRF + PKP I +P H SH+Q ++ICSK + +R RSGGHD+EGL
Sbjct: 65 FSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGL 124
Query: 122 SYVSYL--PFIIVDLINLNSISIEVETNTAWVESGATLGGNFTR--QEGKIN--PTALFL 175
SYVS + PFI++DL + ++I ++ N+AWV+SGAT+G + R ++ K++ P L
Sbjct: 125 SYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCS 184
BLAST of ClCG03G008430 vs. TAIR10
Match:
AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)
HSP 1 Score: 218.4 bits (555), Expect = 8.0e-57
Identity = 110/233 (47.21%), Postives = 152/233 (65.24%), Query Frame = 1
Query: 166 ALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGTGLQPGKALEDLLSK 225
ALF LYIG V L++++ FPELGL K+ C E SW+ES L G++L +L+
Sbjct: 300 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESL-GVLTN 359
Query: 226 ASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQISESETPF 285
T+ S K K D+++ PIP AA++ IW+RL+ + + +++ +P+GG MS+++E ETPF
Sbjct: 360 RERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 419
Query: 286 SHRYGYLYSIGYVINW--EQQGNEAEK-KHLSWIRELYSYMTPFASKSPRAAYVNYKDLD 345
HR G LY I YV W E+ N+ E K+L W+ +Y +MTP+ SKSPR AYVN+KD+D
Sbjct: 420 PHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMD 479
Query: 346 IGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTLS 396
+G KT Y++ WG+KYF NNF RLV +KTRVDP DFF EQSIP ++
Sbjct: 480 LGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530
HSP 2 Score: 129.0 bits (323), Expect = 6.4e-30
Identity = 70/154 (45.45%), Postives = 104/154 (67.53%), Query Frame = 1
Query: 3 SSPIFLSLVFILLSSSSPWVASNSSEKHENFLQCLSHHSPNIS---NVIYTPFNSSYSSI 62
SS +F L+F L + SP V+++ S HE+FL+CLS+ + + VI+T +SS+ SI
Sbjct: 4 SSFLFTILLFSL--NISPLVSAHGSN-HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSI 63
Query: 63 LNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVS 122
L+ +++N RFS TPKP+ IITP S +Q + C++ +G+ +RTRS GH +EGLSY++
Sbjct: 64 LDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIA 123
Query: 123 Y-LPFIIVDLINLNSISIEVETNTAWVESGATLG 153
Y PF ++DL NL SIS++V+ T WV++GAT G
Sbjct: 124 YNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAG 154
BLAST of ClCG03G008430 vs. TAIR10
Match:
AT1G30760.1 (AT1G30760.1 FAD-binding Berberine family protein)
HSP 1 Score: 213.8 bits (543), Expect = 2.0e-55
Identity = 106/222 (47.75%), Postives = 146/222 (65.77%), Query Frame = 1
Query: 172 YIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGTGLQPGKALEDLLSKASLTNA 231
++G L+ ++ +FP+LGL KK C+ETSW++S + + G E LL SL
Sbjct: 319 FLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA-GFPSTAPSEALLDGKSLFKN 378
Query: 232 STKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQISESETPFSHRYGY 291
K KSDY++ PIP+ +EG+W++L ED P +++PYGG+M++I E+ETPF HR G
Sbjct: 379 YFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGMMAKIPETETPFPHRSGT 438
Query: 292 LYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNYKDLDIGSNNRYGK 351
L+ I ++ W Q G +E KH+ W+RE+YSYM + SKSPR+AYVNY+DLD+G N GK
Sbjct: 439 LFKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN---GK 498
Query: 352 TSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPT 394
S A WG +YF NF RLV+IK + DP +FF+HEQSIPT
Sbjct: 499 GS--DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531
HSP 2 Score: 114.8 bits (286), Expect = 1.3e-25
Identity = 72/176 (40.91%), Postives = 105/176 (59.66%), Query Frame = 1
Query: 10 LVFILLSSSSPWVASNSSEKHENFLQCLSHHS----PNISNVIYTPFN-SSYSSILNFTL 69
LV +LL S S ++ + S ++F+ CL +S P S+ N SS+ L +
Sbjct: 13 LVTLLLLSLS-YIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 72
Query: 70 RNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYL--P 129
+NLR+ P+ PKP I P + +H+Q AV+C+K L +R RSGGHD+EGLSYVS +
Sbjct: 73 QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 132
Query: 130 FIIVDLINLNSISIEVETNTAWVESGATLGGNFTR--QEGKIN--PTALFLSLYIG 175
F+IVDL L IS+++E+N+AWV +GA++G + R ++ KI+ P L SL IG
Sbjct: 133 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIG 187
BLAST of ClCG03G008430 vs. TAIR10
Match:
AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)
HSP 1 Score: 213.4 bits (542), Expect = 2.6e-55
Identity = 107/238 (44.96%), Postives = 152/238 (63.87%), Query Frame = 1
Query: 156 TRQEGKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGTGLQP 215
T + G + + + ++G L+ ++ +FPELGL KK C E SW++S + + G
Sbjct: 301 TTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPN 360
Query: 216 GKALEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIM 275
A E LL+ SL K KSD++K PIP+ +EG+W+R ED P +++PYGG+M
Sbjct: 361 SAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMM 420
Query: 276 SQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAY 335
S+ISESE PF HR G L+ I ++ W Q G +E++H+ WIRE+YSYM + SK+PR AY
Sbjct: 421 SRISESEIPFPHRNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAY 480
Query: 336 VNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPT 394
VNY+DLD+G+N G+T ++ WG KY+ NF RLV IK DP +FF+HEQS+PT
Sbjct: 481 VNYRDLDLGTNE--GETDARE---WGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529
HSP 2 Score: 113.6 bits (283), Expect = 2.8e-25
Identity = 68/178 (38.20%), Postives = 104/178 (58.43%), Query Frame = 1
Query: 8 LSLVFILLSSSSPWVASNSSEKHENFLQCLSHHSP---NISNVIYTPFNSS--YSSILNF 67
L LV +LL S V +SS ++F++CL +S I+ ++P ++ + L
Sbjct: 12 LFLVTLLLIS----VPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELES 71
Query: 68 TLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVS--Y 127
T +NLR+ TP+ PKP+ I P + +H+Q AV+C+K L +R RSGGHD+EGLS+V+
Sbjct: 72 TAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDE 131
Query: 128 LPFIIVDLINLNSISIEVETNTAWVESGATLGGNFTRQEGKIN----PTALFLSLYIG 175
PF+IVDL L + +++++N+AW +GAT+G + R + K P L SL IG
Sbjct: 132 TPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIG 185
BLAST of ClCG03G008430 vs. TAIR10
Match:
AT5G44380.1 (AT5G44380.1 FAD-binding Berberine family protein)
HSP 1 Score: 208.0 bits (528), Expect = 1.1e-53
Identity = 105/237 (44.30%), Postives = 148/237 (62.45%), Query Frame = 1
Query: 159 EGKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGTGLQPGKA 218
+G T +L ++G+ L+ ++ FPELGL +K C E SW+E+ L G G G
Sbjct: 307 DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG-GFPTGSP 366
Query: 219 LEDLLS-KASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQ 278
+E LL K+ L K SD++K PIP+ ++GI++RL + L ++PYGG+MS+
Sbjct: 367 IEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYGGMMSK 426
Query: 279 ISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVN 338
I ES PF HR G L+ I Y NW + + +K ++WI+E+Y+YM P+ S +PR AYVN
Sbjct: 427 IPESAIPFPHRNGTLFKILYYANWLENDKTSSRK-INWIKEIYNYMAPYVSSNPRQAYVN 486
Query: 339 YKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTL 395
Y+DLD G N K ++ +A IWG KYF NF+RLV IKT+VDP +FF+HEQSIP +
Sbjct: 487 YRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539
HSP 2 Score: 119.8 bits (299), Expect = 3.9e-27
Identity = 70/182 (38.46%), Postives = 107/182 (58.79%), Query Frame = 1
Query: 4 SPIFLSLVFILLSSSSPWVASNSSEKHENFLQCL---SHHSPNISNVIYTPFN--SSYSS 63
S I +S+++ +P S+S+ + F+ C+ +H S + ++TP S ++
Sbjct: 11 SCIIVSVLYFSFYCITP--TSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQ 70
Query: 64 ILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYV 123
+L T +NL+F + PKP I P H S +Q ++ICSK G+ R RSGGHDFE LSYV
Sbjct: 71 VLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYV 130
Query: 124 SYL--PFIIVDLINLNSISIEVETNTAWVESGATLGGNFTR--QEGKIN--PTALFLSLY 175
S + PFI++DL L I++++E+N+AWV+ GATLG + R ++ KI+ P L S+
Sbjct: 131 SRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVG 190
BLAST of ClCG03G008430 vs. NCBI nr
Match:
gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])
HSP 1 Score: 352.1 bits (902), Expect = 1.3e-93
Identity = 168/244 (68.85%), Postives = 198/244 (81.15%), Query Frame = 1
Query: 152 GGNFTR-QEGKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMG 211
GGN T QEG NP A F SL++GKV ELV+ L+TTFPELGL K+ CIETSW+ESTLI
Sbjct: 294 GGNITTTQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIAS 353
Query: 212 TGLQPGKALEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSP 271
TG+Q ++LE LL++ + KIKSDYIK PI +A +EGIWQRLK +DIE QL+F P
Sbjct: 354 TGVQTVESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIP 413
Query: 272 YGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKS 331
YGG MSQISESETPFSHR G LY IGY++ W++Q +A+KKH+SWIRE+Y YMTPF SKS
Sbjct: 414 YGGRMSQISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKS 473
Query: 332 PRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQS 391
PR+AY NY+DLDIG N +YGKTS KQASIWGLKYFGNNF RLV +KT+VDPYDFF+HEQS
Sbjct: 474 PRSAYANYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQS 533
Query: 392 IPTL 395
IPTL
Sbjct: 534 IPTL 537
BLAST of ClCG03G008430 vs. NCBI nr
Match:
gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])
HSP 1 Score: 215.7 bits (548), Expect = 1.5e-52
Identity = 110/159 (69.18%), Postives = 131/159 (82.39%), Query Frame = 1
Query: 3 SSPIF-LSLVFILLSSSSPWVASNSSEKHENFLQCLSHHSPN---ISNVIYTPFNSSYSS 62
SSP+ SL+ I++SSSS WV++ S KH NFLQCLS HS IS V++TP NSSYSS
Sbjct: 4 SSPLIPFSLILIVVSSSSLWVSA-SPHKHHNFLQCLSEHSSKSYPISKVVHTPINSSYSS 63
Query: 63 ILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYV 122
+LNF++RNLRFS P TPKPLLIITPSH+SHI+ AVICSKS+GLQIRTRSGGHD+EGLSYV
Sbjct: 64 VLNFSIRNLRFSKPETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGLSYV 123
Query: 123 SYLPFIIVDLINLNSISIEVETNTAWVESGATLGGNFTR 158
+ PFII+DLINL S+ I+VE+NTAWVESG TLG + R
Sbjct: 124 ASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYR 161
HSP 2 Score: 352.1 bits (902), Expect = 1.3e-93
Identity = 164/243 (67.49%), Postives = 200/243 (82.30%), Query Frame = 1
Query: 152 GGNFTRQEGKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGT 211
GGN + Q G NPTA F SL++G+ +ELV+ L+T FPELGL K+ C+E SWVESTLI+
Sbjct: 294 GGNASTQAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPI 353
Query: 212 GLQPGKALEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPY 271
G+QP + LE LL++ T STKIKSDYI PIP+AA++ IWQRLK +DIE QL+F PY
Sbjct: 354 GVQPIETLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPY 413
Query: 272 GGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSP 331
GG MSQISESETPFSHR YL+ IGYV+ W+ Q +A+K+H+SW+RE+Y YM PF SKSP
Sbjct: 414 GGRMSQISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSP 473
Query: 332 RAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSI 391
RAAY NY+DLDIGSNN+YGKTSYK+AS+WGLKYFGNNF+RLV +KT+VDPYDFF+HEQSI
Sbjct: 474 RAAYTNYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSI 533
Query: 392 PTL 395
PTL
Sbjct: 534 PTL 536
BLAST of ClCG03G008430 vs. NCBI nr
Match:
gi|659068687|ref|XP_008445684.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])
HSP 1 Score: 219.9 bits (559), Expect = 7.8e-54
Identity = 113/158 (71.52%), Postives = 132/158 (83.54%), Query Frame = 1
Query: 3 SSPIFLSLVFILLSSSSPWVASNSSEKHENFLQCLSHHSPN---ISNVIYTPFNSSYSSI 62
S+ I LSLVFI++SS+S AS S +K++NFLQCLS HS IS V+YTP NSSYSS+
Sbjct: 5 STLIPLSLVFIVVSSAS-LCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSV 64
Query: 63 LNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVS 122
L+F++RNL FS P TPKPLLIITPSH+SHIQ AVICSKS+GLQIRTRSGGHDFEGLSYV+
Sbjct: 65 LDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA 124
Query: 123 YLPFIIVDLINLNSISIEVETNTAWVESGATLGGNFTR 158
Y PFI+VDLINL S+ +EVE NTAWVESGATLG + R
Sbjct: 125 YRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYR 161
HSP 2 Score: 350.1 bits (897), Expect = 5.0e-93
Identity = 167/244 (68.44%), Postives = 199/244 (81.56%), Query Frame = 1
Query: 152 GGNFTRQEGKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGT 211
GGN + Q G PTA F SL++GKV+ELV+ L+TTFPE GL KK C+E W+ESTLI+ +
Sbjct: 296 GGNTSTQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIAS 355
Query: 212 GL-QPGKALEDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSP 271
G+ Q ++L+ LL++ T STKIKSDYIK+PIP AA+EGIWQRLK DIE PQL+F P
Sbjct: 356 GVFQTVESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVP 415
Query: 272 YGGIMSQISESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKS 331
YG MSQISESETPFSHR GYLY IGY++ W+ Q +A+ +H+SW RELY YMTPF SKS
Sbjct: 416 YGARMSQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKS 475
Query: 332 PRAAYVNYKDLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQS 391
PR AYVNY+DLDIGSNN+YGKTSYKQASIWGLKYFGNNF RLV +K +VDP+DFF+HEQS
Sbjct: 476 PRTAYVNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQS 535
Query: 392 IPTL 395
IPTL
Sbjct: 536 IPTL 539
BLAST of ClCG03G008430 vs. NCBI nr
Match:
gi|659068690|ref|XP_008445695.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])
HSP 1 Score: 215.3 bits (547), Expect = 1.9e-52
Identity = 109/164 (66.46%), Postives = 130/164 (79.27%), Query Frame = 1
Query: 1 MKSS----PIFLSLVFILLSSSSPWVASNSSEKHENFLQCLSHHSPN---ISNVIYTPFN 60
MKSS P+F L+ +++SSS WV++ E H+NFL CL HS IS V+YTP N
Sbjct: 1 MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHE-HQNFLHCLPEHSSKSYPISKVVYTPIN 60
Query: 61 SSYSSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFE 120
SSYSS+LNF++RN RFS P TPKPLLIITPSH+SHIQ AVICSKS+GLQIRTRSGGHD+E
Sbjct: 61 SSYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYE 120
Query: 121 GLSYVSYLPFIIVDLINLNSISIEVETNTAWVESGATLGGNFTR 158
GLSYV+Y PFIIVDLIN+ SIS+++ NTAWVESGATLG + R
Sbjct: 121 GLSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYR 163
HSP 2 Score: 349.4 bits (895), Expect = 8.6e-93
Identity = 188/409 (45.97%), Postives = 261/409 (63.81%), Query Frame = 1
Query: 8 LSLVFILLSSSSPWVASNSSEKHENFLQCL---SHHSPNISNVIYTPFNSSYSSILNFTL 67
L +F + S WV + S+ HE+FL+CL S S +IS VIYTP NSSY S+L +
Sbjct: 7 LPFLFAVFLFSFSWVTTTSAHTHEDFLECLKLQSEDSSSISKVIYTPINSSYLSVLQLGI 66
Query: 68 RNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVSYLPFI 127
N RF+T TPKP +I+TP ++SHI+ + CSK + LQIR RSGGHD+EGLSYVS PF+
Sbjct: 67 NNERFNTSTTPKPQVIVTPLNLSHIEATITCSKKHDLQIRIRSGGHDYEGLSYVSPNPFV 126
Query: 128 IVDLINLNSISIEVETNTAWVESGATLGGNF----TRQEGKINPTALFLSLYIG------ 187
I+DLI L SI+++ TAWV+ GAT G + + + P ++ ++ IG
Sbjct: 127 ILDLITLQSITVDATNKTAWVQVGATTGKLYYSIAEKSQNLAFPASVCTTVGIGGHFSGG 186
Query: 188 ------KVEELV--SILNTTFPEL-GLAKKHCIETSWVESTLIMGTGLQPGKALEDLLSK 247
+ L +I++ ++ GL K+ E SW+ES L G +LE LL +
Sbjct: 187 GYGTLLRKYGLAADNIIDAQLTDVDGLTKEDSTEMSWIESVLYF-AGFS-NSSLEILLER 246
Query: 248 ASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQISESETPF 307
K KSDY+++P+P A+EGIW+RL ++ QL+F+ YGG +S+ISES PF
Sbjct: 247 TQQNVRYFKAKSDYVQQPMPEVALEGIWKRLFEVEVGSGQLMFNLYGGRVSEISESSIPF 306
Query: 308 SHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNYKDLDIGS 367
HR G LY I + + W +G E H+SWIR LYS++ P+ SK+PR+AY+NY+DLDIG
Sbjct: 307 PHRAGNLYKIQHSVYWNDEGIEESITHISWIRRLYSFLAPYVSKNPRSAYINYRDLDIGI 366
Query: 368 NNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTL 395
NN G TSY+QASIWG+KYF NF+RLV +KT+VDP +FF++EQSIP+L
Sbjct: 367 NN-IGNTSYEQASIWGIKYFKINFDRLVHVKTQVDPANFFRNEQSIPSL 412
BLAST of ClCG03G008430 vs. NCBI nr
Match:
gi|302143452|emb|CBI22013.3| (unnamed protein product [Vitis vinifera])
HSP 1 Score: 347.1 bits (889), Expect = 4.3e-92
Identity = 190/421 (45.13%), Postives = 261/421 (62.00%), Query Frame = 1
Query: 3 SSPIFLSLVFILLSSSSPWVASNSSEKHENFLQCLS---HHSPNISNVIYTPFNSSYSSI 62
SS IFL V +++ S W S+S HENFLQCLS +H+ ISNV+YTP N SYS+I
Sbjct: 16 SSAIFLPFVSLVVLVS--WATSSSI--HENFLQCLSLNSNHTTPISNVLYTPKNLSYSAI 75
Query: 63 LNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYVS 122
L T+ NLRFS+ TPKPLLI+TP H+SHIQ AVICSK YG+ IR RSGGHD+EGLSY+S
Sbjct: 76 LESTIENLRFSSSATPKPLLILTPLHVSHIQAAVICSKKYGMNIRVRSGGHDYEGLSYLS 135
Query: 123 YLP-------FIIVDLINL--------NSISIEVETNTAW-----VESGATLGGNFTRQE 182
Y + + D N + +E +T++ + LG T++
Sbjct: 136 YCRSGWALQRWGLWDFDEKRGWRSKFWNHLVMENQTSSCSAYCDRLHCWQDLGTRCTQEV 195
Query: 183 GKINPTALFLSLYIGKVEELVSILNTTFPELGLAKKHCIETSWVESTLIMGTGLQPGKAL 242
F SL++G +L+S++ +FPELGL K C+E SW+E
Sbjct: 196 S-------FKSLFLGNTSQLLSLMKKSFPELGLEAKDCLEMSWIEIPQFKNY-------- 255
Query: 243 EDLLSKASLTNASTKIKSDYIKRPIPMAAVEGIWQRLKLEDIEMPQLLFSPYGGIMSQIS 302
K KSDY++ PI ++G+W+ L E+ + ++ SPYGG M++IS
Sbjct: 256 -------------FKAKSDYVQEPISETGLQGVWKMLYQEEAGI--MILSPYGGRMNEIS 315
Query: 303 ESETPFSHRYGYLYSIGYVINWEQQGNEAEKKHLSWIRELYSYMTPFASKSPRAAYVNYK 362
E+E PF HR G LY I Y+++W+++G+ +K ++WIR+LY+YM P+ SK PRAAY+NY+
Sbjct: 316 ETEVPFPHRKGNLYKIQYLVSWDEEGDRVSQKRINWIRKLYAYMAPYVSKFPRAAYLNYR 375
Query: 363 DLDIGSNNRYGKTSYKQASIWGLKYFGNNFNRLVDIKTRVDPYDFFKHEQSIPTLSNKKV 401
DLD+G N G TSY QASIWG+KYF NFNRLV +KT+VDP +FF++EQSIP+LS +
Sbjct: 376 DLDLGINKLKGNTSYAQASIWGIKYFSCNFNRLVHVKTKVDPSNFFRNEQSIPSLSPCSM 402
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| CBDAS_CANSA | 5.2e-58 | 47.58 | Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1 | [more] |
| CASL1_CANSA | 2.0e-57 | 45.75 | Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1 | [more] |
| CASL2_CANSA | 4.4e-57 | 45.75 | Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1 | [more] |
| THCAS_CANSA | 2.9e-56 | 44.31 | Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1 | [more] |
| THCAI_CANSA | 7.1e-55 | 43.09 | Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A067KWX9_JATCU | 6.0e-93 | 45.97 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09769 PE=4 SV=1 | [more] |
| A0A0A0LY37_CUCSA | 1.3e-87 | 62.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_1G539350 PE=4 SV=1 | [more] |
| A0A151RZF5_CAJCA | 1.4e-86 | 43.89 | Reticuline oxidase-like protein OS=Cajanus cajan GN=KK1_030435 PE=4 SV=1 | [more] |
| A0A0A0LVX6_CUCSA | 2.1e-85 | 68.42 | Uncharacterized protein OS=Cucumis sativus GN=Csa_1G539340 PE=4 SV=1 | [more] |
| A0A0A0LYL7_CUCSA | 4.3e-83 | 67.91 | Uncharacterized protein OS=Cucumis sativus GN=Csa_1G539330 PE=4 SV=1 | [more] |
