Cucsa.167330 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.167330 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.1e-145
Identity = 255/513 (49.71%), Postives = 356/513 (69.40%), Query Frame = 1

Query: 11  ESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLH 70
           E+F++CL  Y  ++ TN  A L+YT  +  Y SILN ++QNLRF +   PKP  I TPL+
Sbjct: 32  ENFLKCLSQYIPTNVTN--AKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLN 91

Query: 71  VSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWV 130
           VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS +PFV+VDL N+ S+ IDV +Q AWV
Sbjct: 92  VSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWV 151

Query: 131 HSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYL 190
            SGATLGE+YY I+E ++ L+FPAG CPTVG GGH SGGGYG L+R YGLAAD++IDA+L
Sbjct: 152 ESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHL 211

Query: 191 VDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRI 250
           V+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI    + +E   +
Sbjct: 212 VNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHEL 271

Query: 251 LKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-EGKINPIASFSLMFLGKAEEVLTILK 310
           +KL+++WQ +A   +  LLL        +T+++ + K    + FS +F G  + ++ ++ 
Sbjct: 272 VKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMN 331

Query: 311 PTFPQLGLTKEDCLEMSWIQSVL----LMGWFQKEDPLEVLLNRSRLYSEISKIKSDYVK 370
            +FP+LG+ K DC ++SWI +++    ++ +       E+LL+RS        IK DYVK
Sbjct: 332 KSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVK 391

Query: 371 EHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKW 430
           + IP  A+  + E+L  +DV +   +F PYGG M E+ +S  PF HR+  +Y + YIA W
Sbjct: 392 KPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASW 451

Query: 431 KNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWG 490
           +    E  + H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +AR+WG
Sbjct: 452 E--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWG 511

Query: 491 LKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
            KYFGKNF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 EKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Cucsa.167330 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.9e-145
Identity = 257/517 (49.71%), Postives = 356/517 (68.86%), Query Frame = 1

Query: 11  ESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLH 70
           E+F++C   Y  ++ TN  A L+YT  +  Y SILN +IQNLRF +   PKP  IITPL+
Sbjct: 32  ENFLKCFSQYIPTNVTN--AKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLN 91

Query: 71  VSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWV 130
           VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS +PFV+VDL N+ S+ IDV +Q AWV
Sbjct: 92  VSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWV 151

Query: 131 HSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYL 190
            +GATLGE+YY I+E ++ L+FPAG CPTVG GGH SGGGYG L+R YGLAAD++IDA+L
Sbjct: 152 EAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHL 211

Query: 191 VDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRI 250
           V+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI    + +E   +
Sbjct: 212 VNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHEL 271

Query: 251 LKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-EGKINPIASFSLMFLGKAEEVLTILK 310
           +KL+++WQ +A   +  LLL        +T+++ + K    + FS +F G  + ++ ++ 
Sbjct: 272 VKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMN 331

Query: 311 PTFPQLGLTKEDCLEMSWIQSVLLMG--------WFQKEDPLEVLLNRSRLYSEISKIKS 370
            +FP+LG+ K DC ++SWI +++           +F+K    E+LL+RS        IK 
Sbjct: 332 KSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKK----EILLDRSGGRKAAFSIKL 391

Query: 371 DYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGY 430
           DYVK+ IP  A+  + E+L  +DV +   +F PYGG M E+ +S  PF HR+   Y + Y
Sbjct: 392 DYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWY 451

Query: 431 IAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRA 490
           IA W+    E  + H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +A
Sbjct: 452 IASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQA 511

Query: 491 RVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
           R+WG KYFGKNF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 RIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Cucsa.167330 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.4e-143
Identity = 254/514 (49.42%), Postives = 351/514 (68.29%), Query Frame = 1

Query: 11  ESFVQCLFDYGSSSTNPIAN--LIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPL 70
           E+F++C   +     N +AN  L+YT  +  Y SILN +IQNLRF++   PKP  I+TP 
Sbjct: 32  ENFLKC---FSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPS 91

Query: 71  HVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQNAW 130
           + SHIQA ++CSK  G QIRTRSGGHD EG+SYIS +PFV+VDL N+ SI IDV +Q AW
Sbjct: 92  NNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAW 151

Query: 131 VHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAY 190
           V +GATLGE+YY I+EK++ L+FP G CPTVGVGGH SGGGYG L+R YGLAAD++IDA+
Sbjct: 152 VEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAH 211

Query: 191 LVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDR 250
           LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP++ TI    + +E   
Sbjct: 212 LVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHG 271

Query: 251 ILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-EGKINPIASFSLMFLGKAEEVLTIL 310
           ++KL ++WQ +A K D +L+L        +T++  + K      FS +F G  + ++ ++
Sbjct: 272 LVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLM 331

Query: 311 KPTFPQLGLTKEDCLEMSWIQSVL----LMGWFQKEDPLEVLLNRSRLYSEISKIKSDYV 370
             +FP+LG+ K DC E SWI + +    ++ +       E+LL+RS        IK DYV
Sbjct: 332 NKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYV 391

Query: 371 KEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAK 430
           K+ IP  A+  + E+L  +DV     +  PYGG M E+ +S  PF HR+   Y + Y A 
Sbjct: 392 KKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTAS 451

Query: 431 WKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVW 490
           W+    E  + H+NW+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +AR+W
Sbjct: 452 WE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIW 511

Query: 491 GLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
           G KYFGKNF+RLV VKTKVDP++FFR+EQSIP L
Sbjct: 512 GEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Cucsa.167330 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 6.8e-143
Identity = 247/513 (48.15%), Postives = 351/513 (68.42%), Query Frame = 1

Query: 11  ESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLH 70
           E+F++C  +Y  ++  NP    IYT  +  Y S+LN +IQNLRF +   PKP  I+TP +
Sbjct: 32  ENFLKCFSEYIPNNPANP--KFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSN 91

Query: 71  VSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWV 130
           VSHIQA+++CSK  G QIRTRSGGHD EGLSYIS +PF +VDL N+ ++ +D+ +Q AWV
Sbjct: 92  VSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWV 151

Query: 131 HSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYL 190
            +GATLGE+YY I+E ++  +FP G CPTVGVGGH SGGGYG L+R YGLAAD++IDA+L
Sbjct: 152 EAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHL 211

Query: 191 VDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRI 250
           V+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP++ TI    + +E   +
Sbjct: 212 VNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGL 271

Query: 251 LKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-EGKINPIASFSLMFLGKAEEVLTILK 310
           +KL ++WQ +A K D +L+L        +T++  + K      FS +FLG  + ++ ++ 
Sbjct: 272 VKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMN 331

Query: 311 PTFPQLGLTKEDCLEMSWIQSVL----LMGWFQKEDPLEVLLNRSRLYSEISKIKSDYVK 370
            +FP+LG+ K DC E+SWI + +    ++ +       E+LL+RS        IK DYVK
Sbjct: 332 KSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVK 391

Query: 371 EHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKW 430
           + IP  A+  + E+L  ++V +   +  PYGG M E+ +S  PF HR+   Y + Y A W
Sbjct: 392 KLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATW 451

Query: 431 KNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWG 490
           +    E  + H+NW+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +AR+WG
Sbjct: 452 E--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWG 511

Query: 491 LKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
            KYFGKNF+RLV VKTK DP++FFR+EQSIP L
Sbjct: 512 EKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Cucsa.167330 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.2e-142
Identity = 254/513 (49.51%), Postives = 349/513 (68.03%), Query Frame = 1

Query: 11  ESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLH 70
           E+F++C   Y  +++TN    L+YT  N  Y S+LN +I NLRF +   PKP  I+TP H
Sbjct: 32  ENFLKCFSQYIPNNATN--LKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSH 91

Query: 71  VSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWV 130
           VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS +PFV+VDL N++SI IDV +Q AWV
Sbjct: 92  VSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWV 151

Query: 131 HSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYL 190
            +GATLGE+YY ++EK++ L+  AG CPTV  GGH  GGGYG L+R YGLAAD++IDA+L
Sbjct: 152 EAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHL 211

Query: 191 VDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRI 250
           V+ +GKV +++SMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+    + +E   +
Sbjct: 212 VNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVP-KSTMFSVKKIMEIHEL 271

Query: 251 LKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-EGKINPIASFSLMFLGKAEEVLTILK 310
           +KL+++WQ +A K D +LLL        +T+++ + K      FS +FLG  + ++ ++ 
Sbjct: 272 VKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMN 331

Query: 311 PTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDP----LEVLLNRSRLYSEISKIKSDYVK 370
            +FP+LG+ K DC ++SWI +++        D      E+LL+RS   +   KIK DYVK
Sbjct: 332 KSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVK 391

Query: 371 EHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKW 430
           + IP      + E+L  +D+        PYGG M E+ +S  PF HR+   Y + YI  W
Sbjct: 392 KPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSW 451

Query: 431 KNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWG 490
           +    E  + HLNWIR IY++MTP+VSK+PR AY+NYRDLDIG N+     +Y +AR+WG
Sbjct: 452 E--KQEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWG 511

Query: 491 LKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
            KYFGKNFDRLV VKT VDP++FFR+EQSIP L
Sbjct: 512 EKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Cucsa.167330 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 7.6e-173
Identity = 297/515 (57.67%), Postives = 379/515 (73.59%), Query Frame = 1

Query: 6   STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAII 65
           S    ESF+QCL    S +++ I+ LIYTP NSSYSSIL FSIQNLRF     PKP  I+
Sbjct: 17  SAHTHESFLQCL---DSQNSHSISKLIYTPINSSYSSILQFSIQNLRFNTSSTPKPLVIV 76

Query: 66  TPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQ 125
           TP ++SHIQAA+ICS+ HG QIR RSGGHD+EGLSY+S   FV++DLINL++I+++ EN+
Sbjct: 77  TPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVIDLINLRTINVNAENK 136

Query: 126 NAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVI 185
            AWV SG T+GE+YYRI+EKS+TLAFPA  CPTVGVGGH SGGGYG L+RKYGLAADH+I
Sbjct: 137 TAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYGMLMRKYGLAADHII 196

Query: 186 DAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLE 245
           DA LVD  G++ ++ESMGEDLFWAIRGGGG +FG+V+AWK+ LV  P  VT+    RTLE
Sbjct: 197 DAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSAPPTVTVFNVQRTLE 256

Query: 246 EDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPI--ASFSLMFLGKAEEV 305
           ++   KL+H WQ+VA KL  +L + I L   +V   EEGK N    ASF+ +FLG  + +
Sbjct: 257 QN-ASKLVHRWQFVADKLHEDLFIRIIL--NRVNSTEEGKSNTTIQASFNSLFLGGIDRL 316

Query: 306 LTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKSDYV 365
           L +++ +FP+LGL KEDC+EMSWIQSVL    F     L+VLL+R+       K KSDYV
Sbjct: 317 LPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLDVLLDRTPSTRRNFKAKSDYV 376

Query: 366 KEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAK 425
           KE IP + ++G+WER   +D+    ++F PYGGKMSE+ +S  PF HR+   Y + ++  
Sbjct: 377 KEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFPHRAGNIYKIQHLIY 436

Query: 426 WKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVW 485
           W    + A K H++WIR +Y Y+ P+VSK+PRAAYVNYRDLDIG NN  G TSY++A +W
Sbjct: 437 WDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIGINNHAGNTSYRQASIW 496

Query: 486 GLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLS 519
           GLKYF  NFDRLV VKT VDP++FFR+EQSIP LS
Sbjct: 497 GLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLS 525

BLAST of Cucsa.167330 vs. TrEMBL
Match: V7CLG8_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G200600g PE=4 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 2.3e-169
Identity = 290/522 (55.56%), Postives = 387/522 (74.14%), Query Frame = 1

Query: 1   TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPK 60
           TPS   T E   FVQCL++Y S +TN I+N++YT TNSSYSS+L+ +IQNLRF N  + K
Sbjct: 21  TPSSADTHEK--FVQCLYNY-SHNTNSISNVVYTQTNSSYSSVLDLTIQNLRFSNSSL-K 80

Query: 61  PFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISI 120
           P  I+TPL +SHIQ  +ICS+ HG QIRTRSGGHD+EGLSY++ +PFV++DL+NL+ I++
Sbjct: 81  PHVIVTPLQISHIQTTIICSQRHGLQIRTRSGGHDYEGLSYVAGVPFVILDLLNLRQITV 140

Query: 121 DVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA 180
           D ENQ AWV SGATLGELYY IS+KS+TL FPAG C TVG+GGH SGGGYG+L+RKYGLA
Sbjct: 141 DAENQTAWVQSGATLGELYYSISQKSKTLGFPAGVCATVGIGGHFSGGGYGFLMRKYGLA 200

Query: 181 ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEA 240
           AD+++DA+++D NG + ++++MGEDLFWAIRGGGG SFG++VAWK+KLV VP+ VT+   
Sbjct: 201 ADNILDAHIIDVNGNLLDRKAMGEDLFWAIRGGGGASFGVIVAWKIKLVPVPSTVTVFRL 260

Query: 241 NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA 300
            RTLEE+   ++I +WQ VA KLD  +++ + +   +V   + GK+   ASF  MFLG  
Sbjct: 261 ERTLEEN-ATEIIQKWQLVANKLDERIMIRMDM--ARVNSSQHGKLTIQASFVSMFLGGV 320

Query: 301 EEVLTILKPTFPQLGLTKEDCLEMSWIQSVL-----LMGWFQKEDPLEVLLNRSRLYSEI 360
           EE++ +++ +FP+LGL ++DC E SWI SV+     L+G    E P EVLLNR+++ S  
Sbjct: 321 EELIPLMRKSFPELGLVRKDCNETSWIGSVVFMNAGLIGSTGNEAP-EVLLNRTQIRSGK 380

Query: 361 SKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYS 420
            K KSDYV++ IP+  ++G+W  L    +E  Q+ F PYGG+MS++ +SE PFSHRS Y 
Sbjct: 381 YKGKSDYVRKPIPIDGLRGLWRLLYDDKIEYGQLQFAPYGGRMSDISESEIPFSHRSGYI 440

Query: 421 YLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRT 480
             + Y+  W     EA + H++WIR +Y YM P+VS SPRAAY+NYRDLD+G NN  G T
Sbjct: 441 NHIHYVVVWGEEGNEATQRHMDWIRRLYKYMEPYVSSSPRAAYINYRDLDVGVNNNNGYT 500

Query: 481 SYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
           +Y +A +WG KYF  NF RL  VKTKVDP +FFR+EQSIPTL
Sbjct: 501 NYTQASIWGFKYFNNNFKRLARVKTKVDPHNFFRNEQSIPTL 534

BLAST of Cucsa.167330 vs. TrEMBL
Match: G8A1B3_MEDTR (Reticuline oxidase OS=Medicago truncatula GN=MTR_4g094488 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.1e-168
Identity = 285/513 (55.56%), Postives = 375/513 (73.10%), Query Frame = 1

Query: 6   STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAII 65
           ++   ++F+QCL+ Y  + T+ I+ ++YT TNSSYSSIL FSIQNLRF   + PKP  II
Sbjct: 26  TSPHEDNFLQCLYSYSHNITS-ISKVVYTKTNSSYSSILKFSIQNLRFATNETPKPLVII 85

Query: 66  TPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQ 125
           TP  +SHIQ A+ICS+ HG QIR RSGGHDFEGLS++S++PFV++DL N + I +DVEN+
Sbjct: 86  TPTQISHIQTAIICSQHHGMQIRIRSGGHDFEGLSFVSNVPFVIIDLTNFRGIDVDVENR 145

Query: 126 NAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVI 185
            AWV SGATLGELYY+I++KS+TL FP G CPTVGVGGH SGGGYG L+RKYGLAAD+VI
Sbjct: 146 TAWVQSGATLGELYYKIAQKSKTLGFPGGVCPTVGVGGHFSGGGYGTLLRKYGLAADNVI 205

Query: 186 DAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLE 245
           DA+++D  G+  ++E+MGEDLFWAIRGGGG SFG++V+WK+KLV+VP+ VT+    RTLE
Sbjct: 206 DAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIVSWKIKLVQVPSTVTVFTVPRTLE 265

Query: 246 EDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEG--KINPIASFSLMFLGKAEEV 305
           ++   KL+H+WQ+VA KL+ NL + I L    +   ++G  K   +A F  +FLG  + +
Sbjct: 266 QN-ATKLVHKWQFVAHKLEENLAINIILQRLDLNSSKQGEPKSTVLALFQSLFLGSVDNL 325

Query: 306 LTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKSDYV 365
           L +++  FP+LGL +EDC+EMSWI+SVL +  F + +PLE LLNR+    + SK KSD+V
Sbjct: 326 LPLMEEKFPELGLVREDCVEMSWIESVLYLFRFPEGEPLETLLNRTLAAKDNSKAKSDFV 385

Query: 366 KEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAK 425
           K  IP   ++G+W        E   ++  PYGG M ++ +SE PF HR    Y + Y   
Sbjct: 386 KIPIPETGLEGLWPLFDEDGAEDVLMVLFPYGGIMDKISESEIPFPHRYGTLYKIQYAVH 445

Query: 426 WKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVW 485
           W     E EK H+NWIR++Y YM PFVSKSPRAAY+NYRDLDIG NN  G TSYK+A +W
Sbjct: 446 WHQEGDEVEKLHINWIRKLYSYMEPFVSKSPRAAYINYRDLDIGVNNINGYTSYKQASIW 505

Query: 486 GLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPT 517
           G+KYF  NF RL  VKTKVDP +FFR+EQSIP+
Sbjct: 506 GVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPS 536

BLAST of Cucsa.167330 vs. TrEMBL
Match: V7CLG0_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G199800g PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.6e-167
Identity = 289/518 (55.79%), Postives = 386/518 (74.52%), Query Frame = 1

Query: 1   TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPK 60
           TPS  S    E FVQCL++Y  ++T+ I+N++YT TNSSYSS+L+ SIQNLRF N    K
Sbjct: 21  TPS--SADSHEKFVQCLYNYPHNTTS-ISNVVYTQTNSSYSSVLDVSIQNLRFFNAT-SK 80

Query: 61  PFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISI 120
           P  ++TPL VSHIQA +ICS+ HG QIRTRSGGHD+EGLSY++ +PFV+VDLI L+ I +
Sbjct: 81  PLVVVTPLTVSHIQATIICSQRHGLQIRTRSGGHDYEGLSYVAGVPFVVVDLIKLRGIVV 140

Query: 121 DVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA 180
           DVEN  AWV +GAT+GELYYRIS+KS+ L FPAG CP VGVGGH SGGGYG+L+RK+GLA
Sbjct: 141 DVENSTAWVEAGATIGELYYRISQKSEILGFPAGVCPPVGVGGHFSGGGYGFLMRKFGLA 200

Query: 181 ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEA 240
           AD+VIDA++VD NG + +KE+MGEDLFWAIRGGGG SFG++VAWK+KLV VP+ VT+   
Sbjct: 201 ADNVIDAHIVDVNGNLLDKETMGEDLFWAIRGGGGASFGVIVAWKIKLVPVPSTVTVFRV 260

Query: 241 NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA 300
            RTLEE+   ++IH+WQ VA KL+ +L + I++  G+V   + G +   A F  ++LG+ 
Sbjct: 261 PRTLEEN-ATEIIHKWQRVANKLNESLTIRINM--GRVNSSQNGNLTVQAQFESLYLGRV 320

Query: 301 EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYS-EISKIK 360
           ++++ +++ +FP+LGL +EDC EMSWI+S+L M  F   +  +VLLNR++L      K K
Sbjct: 321 DDLIPLMQNSFPELGLVREDCTEMSWIESILYMAGFTNGESTDVLLNRTQLNGLSFFKAK 380

Query: 361 SDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVG 420
           SDYVK+ IP V ++G+W  L   + E + + F PYGG+M+E+ +SETPF HRS   + + 
Sbjct: 381 SDYVKDPIPDVGLQGLWPFLYEDEAEDAFVQFTPYGGRMNEISESETPFPHRSGNIFHIQ 440

Query: 421 YIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKR 480
           Y   W+     A + H+NWIR +Y+Y   +VSKSPR+AY+NYRDLDIG NN  G TS+ +
Sbjct: 441 YGVSWQEKGDVAAQRHVNWIRRVYNYTEAYVSKSPRSAYLNYRDLDIGVNNN-GYTSFSQ 500

Query: 481 ARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
           AR+WG KYF  NF+RL  VKTKVDP +FFR+EQSIP L
Sbjct: 501 ARIWGTKYFNNNFNRLARVKTKVDPRNFFRNEQSIPPL 530

BLAST of Cucsa.167330 vs. TrEMBL
Match: U5G2R0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16560g PE=4 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 2.8e-167
Identity = 291/514 (56.61%), Postives = 388/514 (75.49%), Query Frame = 1

Query: 6   STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAII 65
           S +  E F+QCL  +   ST  I+ +IYTP NSSYSSIL+F+I+N RF + ++ KPF I+
Sbjct: 24  SARPHEDFLQCLSLHFEDST-AISKVIYTPKNSSYSSILHFAIRNPRFNSSEL-KPFVIV 83

Query: 66  TPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQ 125
           TP   SHIQAA+ CS+ H  +IR RSGGHDFEGLSY+S +PFV+VDLINL+SI++D  N+
Sbjct: 84  TPTDASHIQAAIHCSQKHKLEIRIRSGGHDFEGLSYMSTVPFVIVDLINLRSITVDATNK 143

Query: 126 NAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVI 185
            AWV +GATLGELYYRI+EKS+TLAFPAGSCPT+GVGGH SGGGY  + RKYGLA+D+VI
Sbjct: 144 TAWVQAGATLGELYYRIAEKSRTLAFPAGSCPTIGVGGHFSGGGYSTISRKYGLASDNVI 203

Query: 186 DAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLE 245
           DA L+DA G++ ++ESMGEDLFWAIRGGGG SFG+V+AW+++LVEVP +VT+  A RTLE
Sbjct: 204 DAQLIDAKGRILDRESMGEDLFWAIRGGGGQSFGVVIAWRIELVEVPPKVTVFTAARTLE 263

Query: 246 EDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAEEVLT 305
           ++   KLIH WQYVA +L  ++++ + +   +V   EEGK    A+F  +FLG+ +++L 
Sbjct: 264 QN-ATKLIHRWQYVANQLPEDIIIDVLV--NRVNSSEEGKSTIQAAFFSLFLGEVDQLLL 323

Query: 306 ILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEIS-KIKSDYVK 365
           +++ +FP+LGL K++C EMSWI+SV+ +  F     L VLL+R+     +  K KSDYV+
Sbjct: 324 LMQESFPELGLAKDECTEMSWIESVIYIIGFPSNASLNVLLDRTPQPPSLQFKAKSDYVQ 383

Query: 366 EHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKW 425
           E IP +A +G+W+R   +D+E+ +   +PYGGKM E+ +S TPF HR+   Y+V  +  W
Sbjct: 384 EPIPEIAFEGIWKRFFEKDIEVPEFFMLPYGGKMDEISESSTPFPHRAGNRYIVAPVVYW 443

Query: 426 KNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWG 485
              + EA + HL WIR +Y YMTP+VSK+PRAAYVNYRDLD+G NN  G TSYK+A +WG
Sbjct: 444 SEETKEASQRHLAWIRRLYRYMTPYVSKNPRAAYVNYRDLDLGVNN-LGYTSYKQASIWG 503

Query: 486 LKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLS 519
            KYF  NFDRLV VKT+VDP++FFR+EQSIP LS
Sbjct: 504 RKYFKNNFDRLVRVKTEVDPTNFFRNEQSIPPLS 531

BLAST of Cucsa.167330 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 511.9 bits (1317), Expect = 4.6e-145
Identity = 251/516 (48.64%), Postives = 353/516 (68.41%), Query Frame = 1

Query: 2   PSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKP 61
           P   +     SF+QCL     + +N ++ +I+TP ++S+SS+L  SIQN RF   D+PKP
Sbjct: 26  PVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKP 85

Query: 62  FAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISID 121
             I+TP+  S +Q+AV C++  G  IRTRSGGHD+EGLSY++  PFV++DL NL+SI++D
Sbjct: 86  VLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVD 145

Query: 122 VENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAA 181
           V+N++ WV +GAT+GELYY I +K++TLAFPAG CPTVGVGGH SGGGYG L+RK+GLAA
Sbjct: 146 VDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAA 205

Query: 182 DHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEAN 241
           DHVIDA +VDA G++  +  MGED FWAIRGGGG SF +V++WK+ L+ VP+ VT+    
Sbjct: 206 DHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVT 265

Query: 242 RTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAE 301
           +   E   LK+IH WQ+VA K+  +L + + L        +  K    ASF  ++LG  +
Sbjct: 266 K-FSEQSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMVRASFPGLYLGSVK 325

Query: 302 EVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQK--EDPLEVLLNRSRLYSEISKIK 361
            +L ++   FP+LGL ++DC EMSWI+SV+   WF +  E+P+ VL  R+R  S   K K
Sbjct: 326 NLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEPINVLTKRTRA-SLAFKAK 385

Query: 362 SDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVG 421
           SD+V+E +P  A+  +W RL+  + E +Q++F P+GGKMSE+ D ETPF HR    Y + 
Sbjct: 386 SDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQ 445

Query: 422 YIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKR 481
           Y+  W+    + ++ ++ W+  +YD M+ FV+KSPR AY+N RDLD+G      R+ Y+ 
Sbjct: 446 YLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEE 505

Query: 482 ARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIP 516
            + WG+KYF  NF+RLV VKT VDPSDFF  EQSIP
Sbjct: 506 GKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Cucsa.167330 vs. TAIR10
Match: AT1G30760.1 (AT1G30760.1 FAD-binding Berberine family protein)

HSP 1 Score: 499.6 bits (1285), Expect = 2.3e-141
Identity = 253/519 (48.75%), Postives = 358/519 (68.98%), Query Frame = 1

Query: 3   SFCSTQESESFVQCLFDYGSSSTNPIANLIYTP--TNSSYSSILNFSIQNLRFLNKDIPK 62
           SF + Q+   FV CL D  S ++ P+ +  +T     SS+   L  S QNLR+L    PK
Sbjct: 27  SFSTIQQD--FVMCLVD-NSDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPSNPK 86

Query: 63  PFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIP--FVLVDLINLKSI 122
           P  I  PL+ +H+QAAV+C+K     +R RSGGHD+EGLSY+S++   FV+VDL  L+ I
Sbjct: 87  PEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQI 146

Query: 123 SIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYG 182
           S+D+E+ +AWVH+GA++GE+YYRI EKS+   FPAG C ++G+GGH+ GG YG ++RK+G
Sbjct: 147 SVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFG 206

Query: 183 LAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTIC 242
           L AD+V+DA +VDA+GK+ N+ +MGED+FWAIRGGGGGSFG+++AWK+KLV VP  VT+ 
Sbjct: 207 LGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVF 266

Query: 243 EANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL-TGGKVTEDEEGKINPIASFSLMFL 302
              RTLE+D   KL+++WQ VA KLD +L + + +    K  + +E  I+   S+   FL
Sbjct: 267 TVTRTLEQDG-TKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTIS--TSYQGQFL 326

Query: 303 GKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISK 362
           G A  +L +++ +FPQLGLTK+DCLE SWI+SV+ +  F    P E LL+   L+    K
Sbjct: 327 GDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLFKNYFK 386

Query: 363 IKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYL 422
            KSDYV+E IP+  ++G+WE+L  +D  L+  ++ PYGG M+++ ++ETPF HRS   + 
Sbjct: 387 AKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETETPFPHRSGTLFK 446

Query: 423 VGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSY 482
           + ++  W++G   +E  H+ W+RE+Y YM  +VSKSPR+AYVNYRDLD+G N K      
Sbjct: 447 IQWLTLWQDGKT-SEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGK-----G 506

Query: 483 KRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPT 517
             AR WG +YF  NF+RLV +K K DP +FFRHEQSIPT
Sbjct: 507 SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of Cucsa.167330 vs. TAIR10
Match: AT5G44380.1 (AT5G44380.1 FAD-binding Berberine family protein)

HSP 1 Score: 495.4 bits (1274), Expect = 4.4e-140
Identity = 247/523 (47.23%), Postives = 349/523 (66.73%), Query Frame = 1

Query: 1   TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTN--SSYSSILNFSIQNLRFLNKDI 60
           TP+  S    + F+ C+    +  + P+   ++TP    S ++ +L  + QNL+FL K +
Sbjct: 26  TPTSSSASIQDQFINCV-KRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSM 85

Query: 61  PKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI--PFVLVDLINLK 120
           PKP  I  P+H S +QA++ICSK  G   R RSGGHDFE LSY+S I  PF+L+DL  LK
Sbjct: 86  PKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLK 145

Query: 121 SISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRK 180
            I++D+E+ +AWV  GATLGELYYRI+EKS+   FPAG C +VG+GG+++GGGYG L+RK
Sbjct: 146 QINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRK 205

Query: 181 YGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVT 240
           YGLA D+V+D  +VDANGK+ ++ +MGEDLFWAIRGGGG SFGIV+AWK+KLV VP  VT
Sbjct: 206 YGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVT 265

Query: 241 ICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMF 300
           +    +TLE+D  LK I +WQ ++ K+   + + + L         +G      ++   F
Sbjct: 266 VFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAA----GNDGNKTVTMTYLGQF 325

Query: 301 LGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLN-RSRLYSEI 360
           LG+   +L +++  FP+LGLT++DC EMSWI++ L  G F    P+E+LL  +S L  + 
Sbjct: 326 LGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGKDY 385

Query: 361 SKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYS 420
            K  SD+VKE IP++ +KG+++RL   +     + + PYGG MS++ +S  PF HR+   
Sbjct: 386 FKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYGGMMSKIPESAIPFPHRNGTL 445

Query: 421 YLVGYIAKW-KNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGR 480
           + + Y A W +N    + K  +NWI+EIY+YM P+VS +PR AYVNYRDLD G N    +
Sbjct: 446 FKILYYANWLENDKTSSRK--INWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAK 505

Query: 481 TSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
            ++  A++WG KYF  NFDRLV +KTKVDP +FFRHEQSIP +
Sbjct: 506 VNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

BLAST of Cucsa.167330 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 494.6 bits (1272), Expect = 7.5e-140
Identity = 243/526 (46.20%), Postives = 354/526 (67.30%), Query Frame = 1

Query: 1   TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTN--SSYSSILNFSIQNLRFLNKDI 60
           TP+       + F+ C+    +    P+    + PT   S +S +L  + QNLRFL K +
Sbjct: 23  TPTSSLASLQDQFINCV-QRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSM 82

Query: 61  PKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI--PFVLVDLINLK 120
           PKP  I +P+H SH+QA++ICSK     +R RSGGHD+EGLSY+S I  PF+L+DL  ++
Sbjct: 83  PKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMR 142

Query: 121 SISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRK 180
            ++I++++ +AWV SGAT+GELYYRI+EKS+   FPAG C ++G+GGH++GG YG ++RK
Sbjct: 143 QVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRK 202

Query: 181 YGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVT 240
           YGL AD+V+DA +VDANGK+ ++ +MGED FWAIRGG GGSFGI++AWK+KLV VP  VT
Sbjct: 203 YGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVT 262

Query: 241 ICEANRTLEEDRILKLIHEWQYVAPKLDANL----LLGISLTGGKVTEDEEGKINPIASF 300
           +    +TL++D   K+I +WQ VA KL   L    L  ++ TGG  T           S+
Sbjct: 263 VFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKT--------VTTSY 322

Query: 301 SLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLY 360
           + +FLG    ++ ++K +FP+LGLT +DC+EMSW++S+  +  F    P  VLL     +
Sbjct: 323 NALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPF 382

Query: 361 SEIS-KIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHR 420
            ++S K KSD+VK  IP   ++G++++L  +D+ L  +++ PYGG M+++ +S+ PF HR
Sbjct: 383 PKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHR 442

Query: 421 SDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNK 480
               + V Y+  W + S +    H+NWIR++Y YMTP+VS +PR AYVNYRDLD+G N K
Sbjct: 443 KGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTK 502

Query: 481 YGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
             +T  K+A+VWG  YF  NF+RL+ +K KVDP +FFRHEQSIP +
Sbjct: 503 DVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Cucsa.167330 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-139
Identity = 241/515 (46.80%), Postives = 353/515 (68.54%), Query Frame = 1

Query: 6   STQESESFVQCLFDYGSSSTNPIANLIYTPTNSS--YSSILNFSIQNLRFLNKDIPKPFA 65
           S+   + FV+CL D  S  + PI    ++P  ++  +   L  + QNLR+L    PKP  
Sbjct: 26  SSTLQQDFVKCLVD-NSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVF 85

Query: 66  IITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD--IPFVLVDLINLKSISID 125
           I  PL+ +H+QAAV+C+K     +R RSGGHD+EGLS++++   PFV+VDL  L+ + +D
Sbjct: 86  IFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVD 145

Query: 126 VENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAA 185
           +++ +AW H+GAT+GE+YYRI EKSQT  FPAG C ++G+GGHL GG YG ++RK+GL A
Sbjct: 146 LDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGA 205

Query: 186 DHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEAN 245
           D+V+DA +VDANG++ ++ +MGED+FWAIRGGGGGSFG+++AWK+KLV VPA VT+    
Sbjct: 206 DNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVT 265

Query: 246 RTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAE 305
           +TLE+D   K++++W+ +A KLD +L + + ++    T  + G      S+   FLG + 
Sbjct: 266 KTLEQDG-TKVLYKWEQIADKLDDDLFIRVIISPASKT-TKPGNRTISMSYQAQFLGDSN 325

Query: 306 EVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKSD 365
            +L +++ +FP+LGLTK+DC EMSWI+SV+ +  F      E LL    L+    K KSD
Sbjct: 326 RLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSLFKNHFKAKSD 385

Query: 366 YVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYI 425
           +VKE IP+  ++G+WER   +D  L+  ++ PYGG MS + +SE PF HR+   + + ++
Sbjct: 386 FVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISESEIPFPHRNGTLFKIQWL 445

Query: 426 AKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRAR 485
           + W++G V +E+ H+ WIRE+Y YM  +VSK+PR AYVNYRDLD+GTN   G T    AR
Sbjct: 446 STWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE--GETD---AR 505

Query: 486 VWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPT 517
            WG KY+  NF+RLV +K + DP +FFRHEQS+PT
Sbjct: 506 EWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Cucsa.167330 vs. NCBI nr
Match: gi|778665253|ref|XP_004135742.2| (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis sativus])

HSP 1 Score: 1053.5 bits (2723), Expect = 1.2e-304
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 1

Query: 1   TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPK 60
           TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPK
Sbjct: 28  TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPK 87

Query: 61  PFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISI 120
           PFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISI
Sbjct: 88  PFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISI 147

Query: 121 DVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA 180
           DVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA
Sbjct: 148 DVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA 207

Query: 181 ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEA 240
           ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEA
Sbjct: 208 ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEA 267

Query: 241 NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA 300
           NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA
Sbjct: 268 NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA 327

Query: 301 EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKS 360
           EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKS
Sbjct: 328 EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKS 387

Query: 361 DYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGY 420
           DYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGY
Sbjct: 388 DYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGY 447

Query: 421 IAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRA 480
           IAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRA
Sbjct: 448 IAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRA 507

Query: 481 RVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLS 519
           RVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLS
Sbjct: 508 RVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLS 545

BLAST of Cucsa.167330 vs. NCBI nr
Match: gi|659068701|ref|XP_008445757.1| (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo])

HSP 1 Score: 994.2 bits (2569), Expect = 8.6e-287
Identity = 487/518 (94.02%), Postives = 504/518 (97.30%), Query Frame = 1

Query: 1   TPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPK 60
           T SFC TQE +SFVQCLFDYGS ++NPIAN+IYTP+NSSYSSILNFSIQNLRFLNK+I K
Sbjct: 26  TTSFC-TQECDSFVQCLFDYGSPNSNPIANVIYTPSNSSYSSILNFSIQNLRFLNKNISK 85

Query: 61  PFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISI 120
           P AIITP HVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS +PFV+VDLINL SISI
Sbjct: 86  PLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISGVPFVMVDLINLNSISI 145

Query: 121 DVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA 180
           DVENQNAWV SGATLGELYYRI+EKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA
Sbjct: 146 DVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLA 205

Query: 181 ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEA 240
           ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG FGIVVAWKVKLVEVPARVTICEA
Sbjct: 206 ADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGCFGIVVAWKVKLVEVPARVTICEA 265

Query: 241 NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA 300
           NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA
Sbjct: 266 NRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKA 325

Query: 301 EEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKS 360
           EEVLTILKPTFPQL LTKEDCLEMSWIQSVLLMGWFQK DPLEVLLNR+RLYSEISKIKS
Sbjct: 326 EEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKS 385

Query: 361 DYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGY 420
           DYVKEHIP+VA+KGMWERLKS+DVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGY
Sbjct: 386 DYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGY 445

Query: 421 IAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRA 480
           IAKWKNGSVEAE+ HLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRA
Sbjct: 446 IAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRA 505

Query: 481 RVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLS 519
           RVWGLKYFGKNFD+LV+VKTKVDPSDFFRH+QSIPTLS
Sbjct: 506 RVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPTLS 542

BLAST of Cucsa.167330 vs. NCBI nr
Match: gi|778665234|ref|XP_011648520.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 750.4 bits (1936), Expect = 2.2e-213
Identity = 356/518 (68.73%), Postives = 428/518 (82.63%), Query Frame = 1

Query: 1   TPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIP 60
           +PS+  ST+  E+F+QCL +   ++   I+NLIYTP NSS+ S+LNFSIQNLRF  K  P
Sbjct: 20  SPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLRFSRKQTP 79

Query: 61  KPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSIS 120
           KP AIITP HVSHIQA ++CSKSH  QIR RSGGHDFEGLSY+SD+PF++VDLINL+SI+
Sbjct: 80  KPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDLINLRSIT 139

Query: 121 IDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGL 180
           IDVEN+NAWV SGATLGE YYRI EKSQTLAFPAGSCPTVG+GGHLSGGG+GWL+RKYGL
Sbjct: 140 IDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGWLMRKYGL 199

Query: 181 AADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICE 240
           AAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGGSFGI+VAWKVKLV VPA VTIC 
Sbjct: 200 AADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVPATVTICG 259

Query: 241 ANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGK 300
           + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG  T+ E GKINP A FS  FLGK
Sbjct: 260 SQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ-ESGKINPTALFSSFFLGK 319

Query: 301 AEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIK 360
             E++ IL   FP+L L+KE+C EMSWI++VL M  F  ++P EVLLNR+  +   +KIK
Sbjct: 320 VNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTPPFGLSTKIK 379

Query: 361 SDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVG 420
           SDY+K+ +   A K M +RLK+QD+E++QIMF+PYGG+MSE+ +SE PF HR+   Y +G
Sbjct: 380 SDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPHRAGNIYKLG 439

Query: 421 YIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKR 480
           Y  KWK+ S++AEK HLNWIR+IYDYMTPFVSKSPRA Y NYRDLDIG NNKYG+ +Y  
Sbjct: 440 YYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIGMNNKYGKATYSH 499

Query: 481 ARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
           ARVWG KYFGKNFDRLVH+KTK+DP+DFFR+EQSIP L
Sbjct: 500 ARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 536

BLAST of Cucsa.167330 vs. NCBI nr
Match: gi|659068681|ref|XP_008445658.1| (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis melo])

HSP 1 Score: 745.7 bits (1924), Expect = 5.3e-212
Identity = 355/518 (68.53%), Postives = 428/518 (82.63%), Query Frame = 1

Query: 1   TPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIP 60
           +PS+  ST++ E+F+QCLF+   ++   I+NLIYTP NSS+ S+LNFSIQNLRF  K  P
Sbjct: 22  SPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSFYSVLNFSIQNLRFSRKQTP 81

Query: 61  KPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSIS 120
           KP AIITP HVSHIQAA+ICSKSH  QIR RSGGHDFEGLSY+SD+PF++VDLINL+SI+
Sbjct: 82  KPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLSYVSDVPFIVVDLINLRSIT 141

Query: 121 IDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGL 180
           IDVE +NAWV SGATLGE YYRI EKS+TLAFPAGSCPTVG+GGHLSGGG+GWL+RKYGL
Sbjct: 142 IDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVGIGGHLSGGGFGWLMRKYGL 201

Query: 181 AADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICE 240
           AAD+VIDA  +DANGKV+++ESMGEDLFWAIRGGGGGSFGI+VAWKVKLV VPA VTIC 
Sbjct: 202 AADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGIIVAWKVKLVRVPATVTICG 261

Query: 241 ANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGK 300
           + R+LEE+  +KLIH+WQY+   LD NLLLGISLTGG +T+ E GK NP A FS  FLGK
Sbjct: 262 SKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ-ESGKTNPNALFSSFFLGK 321

Query: 301 AEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIK 360
             E++ IL   FP+L L+KE+C EMSWI++VL+M  F   +P EVLLNR+ L+   +KIK
Sbjct: 322 VNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEPFEVLLNRTPLFGLSTKIK 381

Query: 361 SDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVG 420
           SDY+K+ +   A K M +RLK++D+E++QIMF+PYGG+MSE+  SETPF HR    Y +G
Sbjct: 382 SDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEISKSETPFPHRVGNIYKLG 441

Query: 421 YIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKR 480
           Y  KWK+ S++AEK HL+WIR+IYDYMTPFVSKSPRA Y NYRDLDIG NNKYG+ +Y  
Sbjct: 442 YYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNYRDLDIGINNKYGKATYSH 501

Query: 481 ARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
           ARVWG KYFGKNFDRLVHVKT +DP+DFFR+EQSIP L
Sbjct: 502 ARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPAL 538

BLAST of Cucsa.167330 vs. NCBI nr
Match: gi|659068687|ref|XP_008445684.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 716.8 bits (1849), Expect = 2.6e-203
Identity = 343/513 (66.86%), Postives = 422/513 (82.26%), Query Frame = 1

Query: 6   STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAII 65
           S Q+ ++F+QCL ++ SS + PI+ ++YTP NSSYSS+L+FSI+NL F   + PKP  II
Sbjct: 27  SPQKYQNFLQCLSEH-SSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLLII 86

Query: 66  TPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLVDLINLKSISIDVENQ 125
           TP HVSHIQAAVICSKSHG QIRTRSGGHDFEGLSY++  PF++VDLINL+S+ ++VEN 
Sbjct: 87  TPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVEVENN 146

Query: 126 NAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVI 185
            AWV SGATLGELYYRI EKS+TLAFPAG CPTVG+GGH SGGGYG ++RK+GLAAD+VI
Sbjct: 147 TAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 206

Query: 186 DAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLE 245
           DAYLVDANGKV +KESMGEDLFWAIRGGGGGSFGIVVAWK+KLV VP  VTIC  +R LE
Sbjct: 207 DAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPETVTICSTDRNLE 266

Query: 246 EDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAEEVLT 305
           ED I KLIH WQYV  KLD N+ LGI LTGG  +  + G  NP A F  +FLG+A+E++T
Sbjct: 267 EDAI-KLIHRWQYVGNKLDENIYLGIILTGGNAS-TQAGITNPTARFFSLFLGRADELVT 326

Query: 306 ILKPTFPQLGLTKEDCLEMSWIQSVLLMG-WFQKEDPLEVLLNRSRLYSEISKIKSDYVK 365
            L   FP+LGL K+DC+E SW++S L++    Q  + LE LLNR+    + +KIKSDY+ 
Sbjct: 327 TLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPLLNRTLTTLDSTKIKSDYIT 386

Query: 366 EHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKW 425
           E IP+ A+K +W+RLK+QD+E SQ++FVPYGG+MS++ +SETPFSHR+ Y + +GY+  W
Sbjct: 387 EPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESETPFSHRARYLFKIGYVVGW 446

Query: 426 KNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWG 485
           K+ S++A+K H++W+RE+Y+YM PFVSKSPRAAY NYRDLDIG+NNKYG+TSYKRA VWG
Sbjct: 447 KDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLDIGSNNKYGKTSYKRASVWG 506

Query: 486 LKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 518
           LKYFG NFDRLV+VKTKVDP DFFRHEQSIPTL
Sbjct: 507 LKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536