CSPI01G25210 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G25210 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.9e-147
Identity = 261/538 (48.51%), Postives = 365/538 (67.84%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSIL 72
           +I F +   + +S A P        E+F++CL  Y  ++ TN  A L+YT  +  Y SIL
Sbjct: 14  IIFFFLSFNIQISIANPQ-------ENFLKCLSQYIPTNVTN--AKLVYTQHDQFYMSIL 73

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           N ++QNLRF +   PKP  I TPL+VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS 
Sbjct: 74  NSTVQNLRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQ 133

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PFV+VDL N+ S+ IDV +Q AWV SGATLGE+YY I+E ++ L+FPAG CPTVG GGH
Sbjct: 134 VPFVIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGH 193

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
            SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 194 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 253

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-E 312
           K++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL        +T+++ +
Sbjct: 254 KIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGK 313

Query: 313 GKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL----LMGWFQKE 372
            K    + FSS+F G  + ++ ++  +FP+LG+ K DC ++SWI +++    ++ +    
Sbjct: 314 NKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTN 373

Query: 373 DPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMS 432
              E+LL+RS        IK DYVK+ IP  A+  + E+L  +DV +   +F PYGG M 
Sbjct: 374 FKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMD 433

Query: 433 EVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYV 492
           E+ +S  PF HR+  +Y + YIA W+    E  + H+NWIR +Y++ TP+VS +PR AY+
Sbjct: 434 EISESAIPFPHRAGITYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYL 493

Query: 493 NYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 494 NYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G25210 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 263/542 (48.52%), Postives = 365/542 (67.34%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSIL 72
           +I F +   + +S A P        E+F++C   Y  ++ TN  A L+YT  +  Y SIL
Sbjct: 14  IIFFFLSFNIQISIANPQ-------ENFLKCFSQYIPTNVTN--AKLVYTQHDQFYMSIL 73

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           N +IQNLRF +   PKP  IITPL+VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS 
Sbjct: 74  NSTIQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQ 133

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PFV+VDL N+ S+ IDV +Q AWV +GATLGE+YY I+E ++ L+FPAG CPTVG GGH
Sbjct: 134 VPFVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGH 193

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
            SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 194 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 253

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-E 312
           K++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL        +T+++ +
Sbjct: 254 KIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGK 313

Query: 313 GKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMG--------W 372
            K    + FSS+F G  + ++ ++  +FP+LG+ K DC ++SWI +++           +
Sbjct: 314 NKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTY 373

Query: 373 FQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYG 432
           F+K    E+LL+RS        IK DYVK+ IP  A+  + E+L  +DV +   +F PYG
Sbjct: 374 FKK----EILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYG 433

Query: 433 GKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPR 492
           G M E+ +S  PF HR+   Y + YIA W+    E  + H+NWIR +Y++ TP+VS +PR
Sbjct: 434 GIMDEISESAIPFPHRAGIMYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPR 493

Query: 493 AAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIP 545
            AY+NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTKVDP +FFR+EQSIP
Sbjct: 494 MAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 540

BLAST of CSPI01G25210 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 4.6e-145
Identity = 261/539 (48.42%), Postives = 360/539 (66.79%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIAN--LIYTPTNSSYSSI 72
           +I F +   + +S A P        E+F++C   +     N +AN  L+YT  +  Y SI
Sbjct: 14  IIFFFLSFHIQISIANPR-------ENFLKC---FSKHIPNNVANPKLVYTQHDQLYMSI 73

Query: 73  LNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS 132
           LN +IQNLRF++   PKP  I+TP + SHIQA ++CSK  G QIRTRSGGHD EG+SYIS
Sbjct: 74  LNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYIS 133

Query: 133 DIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGG 192
            +PFVVVDL N+ SI IDV +Q AWV +GATLGE+YY I+EK++ L+FP G CPTVGVGG
Sbjct: 134 QVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGG 193

Query: 193 HLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVA 252
           H SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 194 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 253

Query: 253 WKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE- 312
           WK+KLV VP++ TI    + +E   ++KL ++WQ +A K D +L+L        +T++  
Sbjct: 254 WKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHG 313

Query: 313 EGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL----LMGWFQK 372
           + K      FSS+F G  + ++ ++  +FP+LG+ K DC E SWI + +    ++ +   
Sbjct: 314 KNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTA 373

Query: 373 EDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKM 432
               E+LL+RS        IK DYVK+ IP  A+  + E+L  +DV     +  PYGG M
Sbjct: 374 NFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIM 433

Query: 433 SEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAY 492
            E+ +S  PF HR+   Y + Y A W+    E  + H+NW+R +Y++ TP+VS +PR AY
Sbjct: 434 EEISESAIPFPHRAGIMYELWYTASWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAY 493

Query: 493 VNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           +NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTKVDP++FFR+EQSIP L
Sbjct: 494 LNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G25210 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.3e-144
Identity = 253/538 (47.03%), Postives = 360/538 (66.91%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSIL 72
           +I F +   + +S A P        E+F++C  +Y  ++  NP    IYT  +  Y S+L
Sbjct: 14  IIFFFLSFNIQISIANPQ-------ENFLKCFSEYIPNNPANP--KFIYTQHDQLYMSVL 73

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           N +IQNLRF +   PKP  I+TP +VSHIQA+++CSK  G QIRTRSGGHD EGLSYIS 
Sbjct: 74  NSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQ 133

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PF +VDL N+ ++ +D+ +Q AWV +GATLGE+YY I+E ++  +FP G CPTVGVGGH
Sbjct: 134 VPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGH 193

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
            SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 194 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 253

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-E 312
           K+KLV VP++ TI    + +E   ++KL ++WQ +A K D +L+L        +T++  +
Sbjct: 254 KIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGK 313

Query: 313 GKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL----LMGWFQKE 372
            K      FSS+FLG  + ++ ++  +FP+LG+ K DC E+SWI + +    ++ +    
Sbjct: 314 NKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTAN 373

Query: 373 DPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMS 432
              E+LL+RS        IK DYVK+ IP  A+  + E+L  ++V +   +  PYGG M 
Sbjct: 374 FKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMD 433

Query: 433 EVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYV 492
           E+ +S  PF HR+   Y + Y A W+    E  + H+NW+R +Y++ TP+VS +PR AY+
Sbjct: 434 EISESAIPFPHRAGIMYELWYTATWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYL 493

Query: 493 NYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTK DP++FFR+EQSIP L
Sbjct: 494 NYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CSPI01G25210 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 5.1e-144
Identity = 254/513 (49.51%), Postives = 349/513 (68.03%), Query Frame = 1

Query: 38  ESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLH 97
           E+F++C   Y  +++TN    L+YT  N  Y S+LN +I NLRF +   PKP  I+TP H
Sbjct: 32  ENFLKCFSQYIPNNATN--LKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSH 91

Query: 98  VSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWV 157
           VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS +PFV+VDL N++SI IDV +Q AWV
Sbjct: 92  VSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWV 151

Query: 158 HSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYL 217
            +GATLGE+YY ++EK++ L+  AG CPTV  GGH  GGGYG L+R YGLAAD++IDA+L
Sbjct: 152 EAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHL 211

Query: 218 VDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRI 277
           V+ +GKV +++SMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+    + +E   +
Sbjct: 212 VNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVP-KSTMFSVKKIMEIHEL 271

Query: 278 LKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-EGKINPIASFSSMFLGKAEEVLTILK 337
           +KL+++WQ +A K D +LLL        +T+++ + K      FSS+FLG  + ++ ++ 
Sbjct: 272 VKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMN 331

Query: 338 PAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDP----LEVLLNRSRLYSEISKIKSDYVK 397
            +FP+LG+ K DC ++SWI +++        D      E+LL+RS   +   KIK DYVK
Sbjct: 332 KSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVK 391

Query: 398 ERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKW 457
           + IP      + E+L  +D+        PYGG M E+ +S  PF HR+   Y + YI  W
Sbjct: 392 KPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSW 451

Query: 458 KNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWG 517
           +    E  + HLNWIR IY++MTP+VS +PR AY+NYRDLDIG N+     +Y +AR+WG
Sbjct: 452 E--KQEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWG 511

Query: 518 LKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
            KYFGKNFDRLV VKT VDP++FFR+EQSIP L
Sbjct: 512 EKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of CSPI01G25210 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 4.3e-174
Identity = 301/535 (56.26%), Postives = 388/535 (72.52%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILN 72
           +++F+  ++   S  T    S    ESF+QCL    S +++ I+ LIYTP NSSYSSIL 
Sbjct: 1   MLSFLFFLIFSFSWVT----SAHTHESFLQCL---DSQNSHSISKLIYTPINSSYSSILQ 60

Query: 73  FSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
           FSIQNLRF     PKP  I+TP ++SHIQAA+ICS+ HG QIR RSGGHD+EGLSY+S  
Sbjct: 61  FSIQNLRFNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTF 120

Query: 133 PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
            FVV+DLINL++I+++ EN+ AWV SG T+GE+YYRI+EKS+TLAFPA  CPTVGVGGH 
Sbjct: 121 SFVVIDLINLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHF 180

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG L+RKYGLAADH+IDA LVD  G++ ++ESMGEDLFWAIRGGGG +FG+V+AWK
Sbjct: 181 SGGGYGMLMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWK 240

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGK 312
           + LV  P  VT+    RTLE++   KL+H WQ+VA KL  +L + I L   +V   EEGK
Sbjct: 241 ISLVSAPPTVTVFNVQRTLEQN-ASKLVHRWQFVADKLHEDLFIRIIL--NRVNSTEEGK 300

Query: 313 INPI--ASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLE 372
            N    ASF+S+FLG  + +L +++ +FP+LGL KEDC+EMSWIQSVL    F     L+
Sbjct: 301 SNTTIQASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLD 360

Query: 373 VLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDD 432
           VLL+R+       K KSDYVKE IP + ++G+WER   +D+    ++F PYGGKMSE+ +
Sbjct: 361 VLLDRTPSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISE 420

Query: 433 SETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRD 492
           S  PF HR+   Y + ++  W    + A K H++WIR +Y Y+ P+VS +PRAAYVNYRD
Sbjct: 421 SSIPFPHRAGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRD 480

Query: 493 LDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLS 546
           LDIG NN  G TSY++A +WGLKYF  NFDRLV VKT VDP++FFR+EQSIP LS
Sbjct: 481 LDIGINNHAGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLS 525

BLAST of CSPI01G25210 vs. TrEMBL
Match: G8A1B3_MEDTR (Reticuline oxidase OS=Medicago truncatula GN=MTR_4g094488 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 2.6e-171
Identity = 290/536 (54.10%), Postives = 386/536 (72.01%), Query Frame = 1

Query: 10  PLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSS 69
           PL L   I+++ +  S  + +  ++   ++F+QCL+ Y  + T+ I+ ++YT TNSSYSS
Sbjct: 3   PLRLYLTIVLIAIAFSFTSFAIDTSPHEDNFLQCLYSYSHNITS-ISKVVYTKTNSSYSS 62

Query: 70  ILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYI 129
           IL FSIQNLRF   + PKP  IITP  +SHIQ A+ICS+ HG QIR RSGGHDFEGLS++
Sbjct: 63  ILKFSIQNLRFATNETPKPLVIITPTQISHIQTAIICSQHHGMQIRIRSGGHDFEGLSFV 122

Query: 130 SDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVG 189
           S++PFV++DL N + I +DVEN+ AWV SGATLGELYY+I++KS+TL FP G CPTVGVG
Sbjct: 123 SNVPFVIIDLTNFRGIDVDVENRTAWVQSGATLGELYYKIAQKSKTLGFPGGVCPTVGVG 182

Query: 190 GHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVV 249
           GH SGGGYG L+RKYGLAAD+VIDA+++D  G+  ++E+MGEDLFWAIRGGGG SFG++V
Sbjct: 183 GHFSGGGYGTLLRKYGLAADNVIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIV 242

Query: 250 AWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE 309
           +WK+KLV+VP+ VT+    RTLE++   KL+H+WQ+VA KL+ NL + I L    +   +
Sbjct: 243 SWKIKLVQVPSTVTVFTVPRTLEQN-ATKLVHKWQFVAHKLEENLAINIILQRLDLNSSK 302

Query: 310 EG--KINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKED 369
           +G  K   +A F S+FLG  + +L +++  FP+LGL +EDC+EMSWI+SVL +  F + +
Sbjct: 303 QGEPKSTVLALFQSLFLGSVDNLLPLMEEKFPELGLVREDCVEMSWIESVLYLFRFPEGE 362

Query: 370 PLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSE 429
           PLE LLNR+    + SK KSD+VK  IP   ++G+W        E   ++  PYGG M +
Sbjct: 363 PLETLLNRTLAAKDNSKAKSDFVKIPIPETGLEGLWPLFDEDGAEDVLMVLFPYGGIMDK 422

Query: 430 VDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVN 489
           + +SE PF HR    Y + Y   W     E EK H+NWIR++Y YM PFVS SPRAAY+N
Sbjct: 423 ISESEIPFPHRYGTLYKIQYAVHWHQEGDEVEKLHINWIRKLYSYMEPFVSKSPRAAYIN 482

Query: 490 YRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPT 544
           YRDLDIG NN  G TSYK+A +WG+KYF  NF RL  VKTKVDP +FFR+EQSIP+
Sbjct: 483 YRDLDIGVNNINGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPS 536

BLAST of CSPI01G25210 vs. TrEMBL
Match: V7CLG8_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G200600g PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 4.4e-171
Identity = 291/533 (54.60%), Postives = 394/533 (73.92%), Query Frame = 1

Query: 17  IIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQ 76
           ++  + +  + TPS   T E   FVQCL++Y S +TN I+N++YT TNSSYSS+L+ +IQ
Sbjct: 10  VVTAISLFFSFTPSSADTHEK--FVQCLYNY-SHNTNSISNVVYTQTNSSYSSVLDLTIQ 69

Query: 77  NLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVV 136
           NLRF N  + KP  I+TPL +SHIQ  +ICS+ HG QIRTRSGGHD+EGLSY++ +PFV+
Sbjct: 70  NLRFSNSSL-KPHVIVTPLQISHIQTTIICSQRHGLQIRTRSGGHDYEGLSYVAGVPFVI 129

Query: 137 VDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGG 196
           +DL+NL+ I++D ENQ AWV SGATLGELYY IS+KS+TL FPAG C TVG+GGH SGGG
Sbjct: 130 LDLLNLRQITVDAENQTAWVQSGATLGELYYSISQKSKTLGFPAGVCATVGIGGHFSGGG 189

Query: 197 YGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 256
           YG+L+RKYGLAAD+++DA+++D NG + ++++MGEDLFWAIRGGGG SFG++VAWK+KLV
Sbjct: 190 YGFLMRKYGLAADNILDAHIIDVNGNLLDRKAMGEDLFWAIRGGGGASFGVIVAWKIKLV 249

Query: 257 EVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPI 316
            VP+ VT+    RTLEE+   ++I +WQ VA KLD  +++ + +   +V   + GK+   
Sbjct: 250 PVPSTVTVFRLERTLEEN-ATEIIQKWQLVANKLDERIMIRMDM--ARVNSSQHGKLTIQ 309

Query: 317 ASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL-----LMGWFQKEDPLEV 376
           ASF SMFLG  EE++ +++ +FP+LGL ++DC E SWI SV+     L+G    E P EV
Sbjct: 310 ASFVSMFLGGVEELIPLMRKSFPELGLVRKDCNETSWIGSVVFMNAGLIGSTGNEAP-EV 369

Query: 377 LLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDS 436
           LLNR+++ S   K KSDYV++ IP+  ++G+W  L    +E  Q+ F PYGG+MS++ +S
Sbjct: 370 LLNRTQIRSGKYKGKSDYVRKPIPIDGLRGLWRLLYDDKIEYGQLQFAPYGGRMSDISES 429

Query: 437 ETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDL 496
           E PFSHRS Y   + Y+  W     EA + H++WIR +Y YM P+VS+SPRAAY+NYRDL
Sbjct: 430 EIPFSHRSGYINHIHYVVVWGEEGNEATQRHMDWIRRLYKYMEPYVSSSPRAAYINYRDL 489

Query: 497 DIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           D+G NN  G T+Y +A +WG KYF  NF RL  VKTKVDP +FFR+EQSIPTL
Sbjct: 490 DVGVNNNNGYTNYTQASIWGFKYFNNNFKRLARVKTKVDPHNFFRNEQSIPTL 534

BLAST of CSPI01G25210 vs. TrEMBL
Match: V7CLG0_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G199800g PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 1.1e-169
Identity = 291/528 (55.11%), Postives = 391/528 (74.05%), Query Frame = 1

Query: 18  IIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQN 77
           +  + +L + TPS  S    E FVQCL++Y  ++T+ I+N++YT TNSSYSS+L+ SIQN
Sbjct: 11  VTAIALLFSLTPS--SADSHEKFVQCLYNYPHNTTS-ISNVVYTQTNSSYSSVLDVSIQN 70

Query: 78  LRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVV 137
           LRF N    KP  ++TPL VSHIQA +ICS+ HG QIRTRSGGHD+EGLSY++ +PFVVV
Sbjct: 71  LRFFNAT-SKPLVVVTPLTVSHIQATIICSQRHGLQIRTRSGGHDYEGLSYVAGVPFVVV 130

Query: 138 DLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGY 197
           DLI L+ I +DVEN  AWV +GAT+GELYYRIS+KS+ L FPAG CP VGVGGH SGGGY
Sbjct: 131 DLIKLRGIVVDVENSTAWVEAGATIGELYYRISQKSEILGFPAGVCPPVGVGGHFSGGGY 190

Query: 198 GWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVE 257
           G+L+RK+GLAAD+VIDA++VD NG + +KE+MGEDLFWAIRGGGG SFG++VAWK+KLV 
Sbjct: 191 GFLMRKFGLAADNVIDAHIVDVNGNLLDKETMGEDLFWAIRGGGGASFGVIVAWKIKLVP 250

Query: 258 VPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIA 317
           VP+ VT+    RTLEE+   ++IH+WQ VA KL+ +L + I++  G+V   + G +   A
Sbjct: 251 VPSTVTVFRVPRTLEENAT-EIIHKWQRVANKLNESLTIRINM--GRVNSSQNGNLTVQA 310

Query: 318 SFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSR 377
            F S++LG+ ++++ +++ +FP+LGL +EDC EMSWI+S+L M  F   +  +VLLNR++
Sbjct: 311 QFESLYLGRVDDLIPLMQNSFPELGLVREDCTEMSWIESILYMAGFTNGESTDVLLNRTQ 370

Query: 378 LYS-EISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFS 437
           L      K KSDYVK+ IP V ++G+W  L   + E + + F PYGG+M+E+ +SETPF 
Sbjct: 371 LNGLSFFKAKSDYVKDPIPDVGLQGLWPFLYEDEAEDAFVQFTPYGGRMNEISESETPFP 430

Query: 438 HRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDLDIGTN 497
           HRS   + + Y   W+     A + H+NWIR +Y+Y   +VS SPR+AY+NYRDLDIG N
Sbjct: 431 HRSGNIFHIQYGVSWQEKGDVAAQRHVNWIRRVYNYTEAYVSKSPRSAYLNYRDLDIGVN 490

Query: 498 NKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           N  G TS+ +AR+WG KYF  NF+RL  VKTKVDP +FFR+EQSIP L
Sbjct: 491 NN-GYTSFSQARIWGTKYFNNNFNRLARVKTKVDPRNFFRNEQSIPPL 530

BLAST of CSPI01G25210 vs. TrEMBL
Match: I1KRA7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G079900 PE=4 SV=2)

HSP 1 Score: 602.8 bits (1553), Expect = 4.2e-169
Identity = 300/538 (55.76%), Postives = 388/538 (72.12%), Query Frame = 1

Query: 16  FIIIVVVVLSTATPSFCSTQESESFVQCLFDYG-SSSTNPIANLIYTPTNSSYSSILNFS 75
           F  IVV +L +  PS   TQE+  FVQCL++Y   ++T  I+ ++YT TNSSYSSIL+FS
Sbjct: 8   FAAIVVALLFSFIPSSADTQEN--FVQCLYNYPHDNTTTSISKVVYTQTNSSYSSILDFS 67

Query: 76  IQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPF 135
           IQNLRF N    KP  I+TPL VSH+QA +ICS+ H  QIR RSGGHD+EGLSY+S +PF
Sbjct: 68  IQNLRFYNVT-SKPLVIVTPLEVSHVQATIICSQRHNMQIRIRSGGHDYEGLSYVSQVPF 127

Query: 136 VVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSG 195
           VV+DLINL+ I +DVEN+ AWV +GAT+GELY+ IS+KS TL FPAG CPTVG GG++ G
Sbjct: 128 VVLDLINLREIKVDVENRTAWVQAGATIGELYFSISQKSNTLGFPAGVCPTVGTGGNIGG 187

Query: 196 GGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVK 255
           GGYG+++RKYGLAAD+VIDA +VD NG + ++++MGEDLFWAIRGGGG SFG++VAWKVK
Sbjct: 188 GGYGFMLRKYGLAADNVIDAEIVDVNGNLLDRKAMGEDLFWAIRGGGGASFGVIVAWKVK 247

Query: 256 LVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKIN 315
           LV VP+ VT+    RTLE++   ++IH+WQ VA KLD NL++ I L   +VT  + GK  
Sbjct: 248 LVPVPSTVTVFRVPRTLEQN-ATEIIHKWQLVANKLDDNLMIRIHL--ARVTSSKNGKPT 307

Query: 316 PIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEVLLN 375
             A F S +LG  ++++ +++  FP+LGL KEDC E SWI SVL MG F    P EVLLN
Sbjct: 308 VEAQFESTYLGGVDQLIPLMQKRFPELGLVKEDCTETSWIGSVLFMGNFTISGPPEVLLN 367

Query: 376 RSRLYSEIS-KIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSET 435
           R++L   ++ K KSDYV++ IP V ++ +W      + + + + F PYGG+M E+ +SE 
Sbjct: 368 RTQLVGVLNYKAKSDYVRDPIPDVGLEVLWPLFYEDEAQAAFVQFSPYGGRMYEISESEI 427

Query: 436 PFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDLDI 495
           PF HRS   + + Y   WK    E  + H+NWIR +Y YM P+VS SPRAAY NYRDLDI
Sbjct: 428 PFPHRSGNLFHIQYGVYWKGEGNEEAQKHINWIRRMYSYMEPYVSKSPRAAYFNYRDLDI 487

Query: 496 GTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGISKEL 552
           G NN  G TSY +A VWGLKYF  NF RL  VKTKVDP +FFR+EQSIP+L    ++L
Sbjct: 488 GANNNNGYTSYDQASVWGLKYFLNNFKRLATVKTKVDPLNFFRNEQSIPSLISKGRKL 539

BLAST of CSPI01G25210 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 515.8 bits (1327), Expect = 3.4e-146
Identity = 253/532 (47.56%), Postives = 359/532 (67.48%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILN 72
           ++ F     ++LS   P   +     SF+QCL     + +N ++ +I+TP ++S+SS+L 
Sbjct: 10  VLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSSVLA 69

Query: 73  FSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
            SIQN RF   D+PKP  I+TP+  S +Q+AV C++  G  IRTRSGGHD+EGLSY++  
Sbjct: 70  SSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK 129

Query: 133 PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
           PFV++DL NL+SI++DV+N++ WV +GAT+GELYY I +K++TLAFPAG CPTVGVGGH 
Sbjct: 130 PFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHF 189

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG L+RK+GLAADHVIDA +VDA G++  +  MGED FWAIRGGGG SF +V++WK
Sbjct: 190 SGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWK 249

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGK 312
           + L+ VP+ VT+    +   E   LK+IH WQ+VA K+  +L + + L        +  K
Sbjct: 250 IGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVML--------QRYK 309

Query: 313 INPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQK--EDPLE 372
               ASF  ++LG  + +L ++   FP+LGL ++DC EMSWI+SV+   WF +  E+P+ 
Sbjct: 310 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEPIN 369

Query: 373 VLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDD 432
           VL  R+R  S   K KSD+V+E +P  A+  +W RL+  + E +Q++F P+GGKMSE+ D
Sbjct: 370 VLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIAD 429

Query: 433 SETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRD 492
            ETPF HR    Y + Y+  W+    + ++ ++ W+  +YD M+ FV+ SPR AY+N RD
Sbjct: 430 YETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRD 489

Query: 493 LDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIP 543
           LD+G      R+ Y+  + WG+KYF  NF+RLV VKT VDPSDFF  EQSIP
Sbjct: 490 LDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25210 vs. TAIR10
Match: AT1G30760.1 (AT1G30760.1 FAD-binding Berberine family protein)

HSP 1 Score: 501.9 bits (1291), Expect = 5.1e-142
Identity = 253/534 (47.38%), Postives = 365/534 (68.35%), Query Frame = 1

Query: 15  AFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTP--TNSSYSSILN 74
           AF++ ++++  +  P   ST + + FV CL D  S ++ P+ +  +T     SS+   L 
Sbjct: 11  AFLVTLLLLSLSYIPLSFSTIQQD-FVMCLVD-NSDASFPMDSSFFTHDLNASSFKLALE 70

Query: 75  FSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 134
            S QNLR+L    PKP  I  PL+ +H+QAAV+C+K     +R RSGGHD+EGLSY+S++
Sbjct: 71  TSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEM 130

Query: 135 P--FVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGG 194
              FV+VDL  L+ IS+D+E+ +AWVH+GA++GE+YYRI EKS+   FPAG C ++G+GG
Sbjct: 131 ETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGG 190

Query: 195 HLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVA 254
           H+ GG YG ++RK+GL AD+V+DA +VDA+GK+ N+ +MGED+FWAIRGGGGGSFG+++A
Sbjct: 191 HIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILA 250

Query: 255 WKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL-TGGKVTEDE 314
           WK+KLV VP  VT+    RTLE+D   KL+++WQ VA KLD +L + + +    K  + +
Sbjct: 251 WKIKLVPVPEIVTVFTVTRTLEQDG-TKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSK 310

Query: 315 EGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPL 374
           E  I+   S+   FLG A  +L +++ +FPQLGL K+DCLE SWI+SV+ +  F    P 
Sbjct: 311 ERTIS--TSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPS 370

Query: 375 EVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVD 434
           E LL+   L+    K KSDYV+E IP+  ++G+WE+L  +D  L+  ++ PYGG M+++ 
Sbjct: 371 EALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIP 430

Query: 435 DSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYR 494
           ++ETPF HRS   + + ++  W++G   +E  H+ W+RE+Y YM  +VS SPR+AYVNYR
Sbjct: 431 ETETPFPHRSGTLFKIQWLTLWQDGKT-SEAKHMGWMREMYSYMEQYVSKSPRSAYVNYR 490

Query: 495 DLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPT 544
           DLD+G N K        AR WG +YF  NF+RLV +K K DP +FFRHEQSIPT
Sbjct: 491 DLDLGMNGK-----GSDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of CSPI01G25210 vs. TAIR10
Match: AT5G44380.1 (AT5G44380.1 FAD-binding Berberine family protein)

HSP 1 Score: 500.7 bits (1288), Expect = 1.1e-141
Identity = 253/543 (46.59%), Postives = 360/543 (66.30%), Query Frame = 1

Query: 10  PLGLIAFIIIVVVVLS--TATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTN--S 69
           PL  I+ II+ V+  S    TP+  S    + F+ C+    +  + P+   ++TP    S
Sbjct: 6   PLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCV-KRNTHVSFPLEKTLFTPAKNVS 65

Query: 70  SYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEG 129
            ++ +L  + QNL+FL K +PKP  I  P+H S +QA++ICSK  G   R RSGGHDFE 
Sbjct: 66  LFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEA 125

Query: 130 LSYISDI--PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSC 189
           LSY+S I  PF+++DL  LK I++D+E+ +AWV  GATLGELYYRI+EKS+   FPAG C
Sbjct: 126 LSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLC 185

Query: 190 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 249
            +VG+GG+++GGGYG L+RKYGLA D+V+D  +VDANGK+ ++ +MGEDLFWAIRGGGG 
Sbjct: 186 TSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGA 245

Query: 250 SFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 309
           SFGIV+AWK+KLV VP  VT+    +TLE+D  LK I +WQ ++ K+   + + + L   
Sbjct: 246 SFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAA 305

Query: 310 KVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWF 369
                 +G      ++   FLG+   +L +++ AFP+LGL ++DC EMSWI++ L  G F
Sbjct: 306 ----GNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGF 365

Query: 370 QKEDPLEVLLN-RSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYG 429
               P+E+LL  +S L  +  K  SD+VKE IP++ +KG+++RL   +     + + PYG
Sbjct: 366 PTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYG 425

Query: 430 GKMSEVDDSETPFSHRSDYSYLVGYIAKW-KNGSVEAEKSHLNWIREIYDYMTPFVSNSP 489
           G MS++ +S  PF HR+   + + Y A W +N    + K  +NWI+EIY+YM P+VS++P
Sbjct: 426 GMMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRK--INWIKEIYNYMAPYVSSNP 485

Query: 490 RAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSI 545
           R AYVNYRDLD G N    + ++  A++WG KYF  NFDRLV +KTKVDP +FFRHEQSI
Sbjct: 486 RQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSI 539

BLAST of CSPI01G25210 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 498.0 bits (1281), Expect = 7.3e-141
Identity = 247/544 (45.40%), Postives = 365/544 (67.10%), Query Frame = 1

Query: 10  PLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTN--SSY 69
           PL L + I+++   L T TP+       + F+ C+    +    P+    + PT   S +
Sbjct: 6   PLPLFS-ILVLYFSLYTITPTSSLASLQDQFINCV-QRNTHVYFPLEKTFFAPTKNVSMF 65

Query: 70  SSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLS 129
           S +L  + QNLRFL K +PKP  I +P+H SH+QA++ICSK     +R RSGGHD+EGLS
Sbjct: 66  SQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 125

Query: 130 YISDI--PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPT 189
           Y+S I  PF+++DL  ++ ++I++++ +AWV SGAT+GELYYRI+EKS+   FPAG C +
Sbjct: 126 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 185

Query: 190 VGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSF 249
           +G+GGH++GG YG ++RKYGL AD+V+DA +VDANGK+ ++ +MGED FWAIRGG GGSF
Sbjct: 186 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 245

Query: 250 GIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANL----LLGISLT 309
           GI++AWK+KLV VP  VT+    +TL++D   K+I +WQ VA KL   L    L  ++ T
Sbjct: 246 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT 305

Query: 310 GGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMG 369
           GG  T           S++++FLG    ++ ++K +FP+LGL  +DC+EMSW++S+  + 
Sbjct: 306 GGNKT--------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYIS 365

Query: 370 WFQKEDPLEVLLNRSRLYSEIS-KIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 429
            F    P  VLL     + ++S K KSD+VK  IP   ++G++++L  +D+ L  +++ P
Sbjct: 366 GFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNP 425

Query: 430 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 489
           YGG M+++ +S+ PF HR    + V Y+  W + S +    H+NWIR++Y YMTP+VS++
Sbjct: 426 YGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSN 485

Query: 490 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 545
           PR AYVNYRDLD+G N K  +T  K+A+VWG  YF  NF+RL+ +K KVDP +FFRHEQS
Sbjct: 486 PREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQS 536

BLAST of CSPI01G25210 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 496.9 bits (1278), Expect = 1.6e-140
Identity = 244/533 (45.78%), Postives = 361/533 (67.73%), Query Frame = 1

Query: 18  IIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSS--YSSILNFSI 77
           + +V +L  + P   ST + + FV+CL D  S  + PI    ++P  ++  +   L  + 
Sbjct: 12  LFLVTLLLISVPLSSSTLQQD-FVKCLVD-NSDVSFPITASFFSPDQNATLFKEELESTA 71

Query: 78  QNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD--IP 137
           QNLR+L    PKP  I  PL+ +H+QAAV+C+K     +R RSGGHD+EGLS++++   P
Sbjct: 72  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 131

Query: 138 FVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLS 197
           FV+VDL  L+ + +D+++ +AW H+GAT+GE+YYRI EKSQT  FPAG C ++G+GGHL 
Sbjct: 132 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 191

Query: 198 GGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKV 257
           GG YG ++RK+GL AD+V+DA +VDANG++ ++ +MGED+FWAIRGGGGGSFG+++AWK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 258 KLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKI 317
           KLV VPA VT+    +TLE+D   K++++W+ +A KLD +L + + ++    T  + G  
Sbjct: 252 KLVPVPATVTVFTVTKTLEQDG-TKVLYKWEQIADKLDDDLFIRVIISPASKT-TKPGNR 311

Query: 318 NPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEVLL 377
               S+ + FLG +  +L +++ +FP+LGL K+DC EMSWI+SV+ +  F      E LL
Sbjct: 312 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL 371

Query: 378 NRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSET 437
               L+    K KSD+VKE IP+  ++G+WER   +D  L+  ++ PYGG MS + +SE 
Sbjct: 372 AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISESEI 431

Query: 438 PFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDLDI 497
           PF HR+   + + +++ W++G V +E+ H+ WIRE+Y YM  +VS +PR AYVNYRDLD+
Sbjct: 432 PFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 491

Query: 498 GTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSG 547
           GTN   G T    AR WG KY+  NF+RLV +K + DP +FFRHEQS+PT  G
Sbjct: 492 GTNE--GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKIG 532

BLAST of CSPI01G25210 vs. NCBI nr
Match: gi|778665253|ref|XP_004135742.2| (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis sativus])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 548/556 (98.56%), Postives = 550/556 (98.92%), Query Frame = 1

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           MKCFPILPAP GLIAFIII+VVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY
Sbjct: 1   MKCFPILPAPFGLIAFIIIIVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYISDIPFV+VDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA
Sbjct: 121 HDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASFS MFLGKAEEVLTILKP FPQLGL KEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKE IPMVAVKGMWERLKSQDVELSQIMFVP
Sbjct: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVS S
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540

Query: 541 IPTLSGISKELFSASL 557
           IPTLSGISKELFSASL
Sbjct: 541 IPTLSGISKELFSASL 556

BLAST of CSPI01G25210 vs. NCBI nr
Match: gi|659068701|ref|XP_008445757.1| (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo])

HSP 1 Score: 1036.2 bits (2678), Expect = 2.1e-299
Identity = 514/556 (92.45%), Postives = 532/556 (95.68%), Query Frame = 1

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           MKCFPIL AP GLIAFII  VVVLSTAT SFC TQE +SFVQCLFDYGS ++NPIAN+IY
Sbjct: 1   MKCFPILAAPFGLIAFII--VVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+I KP AIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASFS MFLGKAEEVLTILKP FPQL L KEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQK DPLEVLLNR+RLYSEISKIKSDYVKE IP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDYMTPFVS S
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFD+LV+VKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540

Query: 541 IPTLSGISKELFSASL 557
           IPTLSGIS+E FSASL
Sbjct: 541 IPTLSGISEESFSASL 553

BLAST of CSPI01G25210 vs. NCBI nr
Match: gi|778665234|ref|XP_011648520.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 760.0 bits (1961), Expect = 2.9e-216
Identity = 360/527 (68.31%), Postives = 433/527 (82.16%), Query Frame = 1

Query: 22  VVLSTATPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRF 81
           +VLS  +PS+  ST+  E+F+QCL +   ++   I+NLIYTP NSS+ S+LNFSIQNLRF
Sbjct: 14  IVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLRF 73

Query: 82  LNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLI 141
             K  PKP AIITP HVSHIQA ++CSKSH  QIR RSGGHDFEGLSY+SD+PF++VDLI
Sbjct: 74  SRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDLI 133

Query: 142 NLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWL 201
           NL+SI+IDVEN+NAWV SGATLGE YYRI EKSQTLAFPAGSCPTVG+GGHLSGGG+GWL
Sbjct: 134 NLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGWL 193

Query: 202 IRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPA 261
           +RKYGLAAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGGSFGI+VAWKVKLV VPA
Sbjct: 194 MRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVPA 253

Query: 262 RVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFS 321
            VTIC + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG  T+ E GKINP A FS
Sbjct: 254 TVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ-ESGKINPTALFS 313

Query: 322 SMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYS 381
           S FLGK  E++ IL   FP+L L+KE+C EMSWI++VL M  F  ++P EVLLNR+  + 
Sbjct: 314 SFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTPPFG 373

Query: 382 EISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSD 441
             +KIKSDY+K+ +   A K M +RLK+QD+E++QIMF+PYGG+MSE+ +SE PF HR+ 
Sbjct: 374 LSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPHRAG 433

Query: 442 YSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDLDIGTNNKYG 501
             Y +GY  KWK+ S++AEK HLNWIR+IYDYMTPFVS SPRA Y NYRDLDIG NNKYG
Sbjct: 434 NIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIGMNNKYG 493

Query: 502 RTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGI 548
           + +Y  ARVWG KYFGKNFDRLVH+KTK+DP+DFFR+EQSIP L  I
Sbjct: 494 KATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 539

BLAST of CSPI01G25210 vs. NCBI nr
Match: gi|659068681|ref|XP_008445658.1| (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis melo])

HSP 1 Score: 751.9 bits (1940), Expect = 8.0e-214
Identity = 360/536 (67.16%), Postives = 436/536 (81.34%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSIL 72
           L+    +  +VL+  +PS+  ST++ E+F+QCLF+   ++   I+NLIYTP NSS+ S+L
Sbjct: 7   LLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSFYSVL 66

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           NFSIQNLRF  K  PKP AIITP HVSHIQAA+ICSKSH  QIR RSGGHDFEGLSY+SD
Sbjct: 67  NFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLSYVSD 126

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PF+VVDLINL+SI+IDVE +NAWV SGATLGE YYRI EKS+TLAFPAGSCPTVG+GGH
Sbjct: 127 VPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVGIGGH 186

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
           LSGGG+GWL+RKYGLAAD+VIDA  +DANGKV+++ESMGEDLFWAIRGGGGGSFGI+VAW
Sbjct: 187 LSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGIIVAW 246

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEG 312
           KVKLV VPA VTIC + R+LEE+  +KLIH+WQY+   LD NLLLGISLTGG +T+ E G
Sbjct: 247 KVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ-ESG 306

Query: 313 KINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEV 372
           K NP A FSS FLGK  E++ IL   FP+L L+KE+C EMSWI++VL+M  F   +P EV
Sbjct: 307 KTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEPFEV 366

Query: 373 LLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDS 432
           LLNR+ L+   +KIKSDY+K+ +   A K M +RLK++D+E++QIMF+PYGG+MSE+  S
Sbjct: 367 LLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEISKS 426

Query: 433 ETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDL 492
           ETPF HR    Y +GY  KWK+ S++AEK HL+WIR+IYDYMTPFVS SPRA Y NYRDL
Sbjct: 427 ETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNYRDL 486

Query: 493 DIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGI 548
           DIG NNKYG+ +Y  ARVWG KYFGKNFDRLVHVKT +DP+DFFR+EQSIP L  I
Sbjct: 487 DIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDI 541

BLAST of CSPI01G25210 vs. NCBI nr
Match: gi|659068687|ref|XP_008445684.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 725.3 bits (1871), Expect = 8.0e-206
Identity = 349/533 (65.48%), Postives = 432/533 (81.05%), Query Frame = 1

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILN 72
           LI   ++ +VV S +  +  S Q+ ++F+QCL ++ SS + PI+ ++YTP NSSYSS+L+
Sbjct: 7   LIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEH-SSKSYPISKVVYTPINSSYSSVLD 66

Query: 73  FSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
           FSI+NL F   + PKP  IITP HVSHIQAAVICSKSHG QIRTRSGGHDFEGLSY++  
Sbjct: 67  FSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYR 126

Query: 133 PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
           PF+VVDLINL+S+ ++VEN  AWV SGATLGELYYRI EKS+TLAFPAG CPTVG+GGH 
Sbjct: 127 PFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHF 186

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG ++RK+GLAAD+VIDAYLVDANGKV +KESMGEDLFWAIRGGGGGSFGIVVAWK
Sbjct: 187 SGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWK 246

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGK 312
           +KLV VP  VTIC  +R LEED I KLIH WQYV  KLD N+ LGI LTGG  +  + G 
Sbjct: 247 IKLVPVPETVTICSTDRNLEEDAI-KLIHRWQYVGNKLDENIYLGIILTGGNAS-TQAGI 306

Query: 313 INPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMG-WFQKEDPLEV 372
            NP A F S+FLG+A+E++T L   FP+LGL K+DC+E SW++S L++    Q  + LE 
Sbjct: 307 TNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEP 366

Query: 373 LLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDS 432
           LLNR+    + +KIKSDY+ E IP+ A+K +W+RLK+QD+E SQ++FVPYGG+MS++ +S
Sbjct: 367 LLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISES 426

Query: 433 ETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDL 492
           ETPFSHR+ Y + +GY+  WK+ S++A+K H++W+RE+Y+YM PFVS SPRAAY NYRDL
Sbjct: 427 ETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDL 486

Query: 493 DIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           DIG+NNKYG+TSYKRA VWGLKYFG NFDRLV+VKTKVDP DFFRHEQSIPTL
Sbjct: 487 DIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536