Carg26411 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg26411
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	transcription factor bHLH95-like
Location	Cucurbita_argyrosperma_scaffold_257 : 127305 .. 129371 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCCCTCACAATATTTGGGCCTTCCTCAACTCCGGCAGTGATGTCGATATCGACATCGATCTTGACGATGATAATGACAACGACAATAGCAACGACAAATCCCCTGCCAACACCGACCTCTTACCTCCCCTGCCTTTGTCGGGAAGGAAGAGGGAGGCAGCGGAGGGGAGACCGGGTGGAGGTGGAGAGTCGACCGAACATGAAATTCATATTTGGACGGAGAGGGAGAGGAGGAAGAGGATGAGGAATATGTTCTCTAATCTTCATGCTTTGATTCCTCACCTTCCTCCTAAGGTAACTTTTTTTTCTTTTTTTTTCTTTTTTTTTTTTTTTTGTTTTTGTTTTTGTTTTGTTCATCTCATCCATTGAACAAAACTCTTGTTTTTGTAAATTTTGAACTTTTATATATTTTTTTAATTGTTTAATTGTGATATTATATGTCAAAATCAGCTTGGTTGAACTATAATTAACATATATTTTTAATTAAAAAGTCAATGGTTCAAGGAGTTTATTAATTTTTTTTTTTTTTTAAAAAAAAAAAAAATTCTGGGTCCATATAGAGTGATGTAACCTAACAAACTGCCATGTACACGAGTTGAGTTGAGTTGAAGAAAGTTTTTGGATTGGTTGGATTAGTGATTCAAGTACTCTGCATAGAATTCACAAACCAACCGCTCGGTTAAATTGGGTTGTCGGGTTATTTTTTTTAATTATTTTTTTAAAAAAATCTACTTATCTACCCTACACGATACATTAATAATTAAAATCTCAAAAAAAAAAATCAAGTATGAAATATTCAGAAGTTATAATGAGTCATGAATGATACAAACATCAGATATTTTTAATGTTTATAATATCTGTAGAATAGGGTAGGGTTGGGTTTTAACCTCTGATTGAAATTTTTTTTTTAAATCTTAAATTTATGTTATGTATATGATATTTATTTTGTGAAATAATAATTCAATAAGGGAACGAATGAAATGGCAGGCAGACAAATCAACTATAGTGGACGAAGCAGTAAACTACATAAAAACTGTACAACAAACTCTTCAAACACTCTACAACCAAAAGCTCGAAAAGCTCCAAAACAACAATCCAGCTGTGAGCTATAAGGCTGCTCATTATCATCCTCAACAACAACAGCAACAACAACAATTCAGCTCAACCACCACAACCCACCAAGCTTTCTTGGCCGATCAAGCTTCTTCCTCCAACGGCATGTCCTCCACTCTTCTTCCACCACCACTGCCACCACTGCCGCCGCTGCCGCCTCCCTTTTCTGATACTACTACGACGTTTCAGACTTGGACTTCCTCCCACCTCGTATTATGTATTTGTGGCGGTGAAGCCCATTTCTGCATCTGCTCCGTCAAGAAGTCTGGCTTGTTCGCCGCCGTTTGTTATATTCTTGATAAGCATCAGATCGAGGTTGTTTCGGCTCATGTCTCCTCGGATTTCCATCGCACGTTCTTTATGATTCAAGGCCATGTAAGTGCGACCTCTCTATGCATCTTTGTTTTTCATTTTTTCCTTTACAAAGTTTGAATTTTAGTGTGGGATCGCCCGTCCATTGGAGAGAAAAACAAAACATTCCTTATAAGGGTGTGGAAACCTCTCATTAGTATACGCGTTTTAAAAACTTTGAGGGAAAGCCCGAAAGGAAAAGTACAAAGAAGACAATATCTACTCGCAGAAGTGGGCTTGGACGGTTACATTTAGAAATCTCCTCCATTCTTTTCTTGATTTGAACAAAGATTTCGTTCATTGTTTCATGTTTTATTTTCTAATTATAAAATTGCACGCTTTATTTTCTAATTGTAAAATTGTAATGAATGGTGCAAATTATAATGGAATAGTTTTTTATCGAAAGAGGTAAAAAGAGAATCGAAACCGAACTCTAATAGATTCTTATCGAGTTAATTTCTAAGAGTTGTTATTTGAAATGAGTTGTTTTGACGAGAGCAGGTGAATGGAAGTTGGGACGAATTCGGGGCAGCGATGGTGGCAGAAGAAATGTTCAAGCAAGCTGCGGGGGAGATCAACTTCTGGTTGTCTTCTTGA

mRNA sequence

ATGGCTGCCCCTCACAATATTTGGGCCTTCCTCAACTCCGGCAGTGATGTCGATATCGACATCGATCTTGACGATGATAATGACAACGACAATAGCAACGACAAATCCCCTGCCAACACCGACCTCTTACCTCCCCTGCCTTTGTCGGGAAGGAAGAGGGAGGCAGCGGAGGGGAGACCGGGTGGAGGTGGAGAGTCGACCGAACATGAAATTCATATTTGGACGGAGAGGGAGAGGAGGAAGAGGATGAGGAATATGTTCTCTAATCTTCATGCTTTGATTCCTCACCTTCCTCCTAAGGCAGACAAATCAACTATAGTGGACGAAGCAGTAAACTACATAAAAACTGTACAACAAACTCTTCAAACACTCTACAACCAAAAGCTCGAAAAGCTCCAAAACAACAATCCAGCTGTGAGCTATAAGGCTGCTCATTATCATCCTCAACAACAACAGCAACAACAACAATTCAGCTCAACCACCACAACCCACCAAGCTTTCTTGGCCGATCAAGCTTCTTCCTCCAACGGCATGTCCTCCACTCTTCTTCCACCACCACTGCCACCACTGCCGCCGCTGCCGCCTCCCTTTTCTGATACTACTACGACGTTTCAGACTTGGACTTCCTCCCACCTCGTATTATGTATTTGTGGCGGTGAAGCCCATTTCTGCATCTGCTCCGTCAAGAAGTCTGGCTTGTTCGCCGCCGTTTGTTATATTCTTGATAAGCATCAGATCGAGGTTGTTTCGGCTCATGTCTCCTCGGATTTCCATCGCACGTTCTTTATGATTCAAGGCCATGTGAATGGAAGTTGGGACGAATTCGGGGCAGCGATGGTGGCAGAAGAAATGTTCAAGCAAGCTGCGGGGGAGATCAACTTCTGGTTGTCTTCTTGA

Coding sequence (CDS)

ATGGCTGCCCCTCACAATATTTGGGCCTTCCTCAACTCCGGCAGTGATGTCGATATCGACATCGATCTTGACGATGATAATGACAACGACAATAGCAACGACAAATCCCCTGCCAACACCGACCTCTTACCTCCCCTGCCTTTGTCGGGAAGGAAGAGGGAGGCAGCGGAGGGGAGACCGGGTGGAGGTGGAGAGTCGACCGAACATGAAATTCATATTTGGACGGAGAGGGAGAGGAGGAAGAGGATGAGGAATATGTTCTCTAATCTTCATGCTTTGATTCCTCACCTTCCTCCTAAGGCAGACAAATCAACTATAGTGGACGAAGCAGTAAACTACATAAAAACTGTACAACAAACTCTTCAAACACTCTACAACCAAAAGCTCGAAAAGCTCCAAAACAACAATCCAGCTGTGAGCTATAAGGCTGCTCATTATCATCCTCAACAACAACAGCAACAACAACAATTCAGCTCAACCACCACAACCCACCAAGCTTTCTTGGCCGATCAAGCTTCTTCCTCCAACGGCATGTCCTCCACTCTTCTTCCACCACCACTGCCACCACTGCCGCCGCTGCCGCCTCCCTTTTCTGATACTACTACGACGTTTCAGACTTGGACTTCCTCCCACCTCGTATTATGTATTTGTGGCGGTGAAGCCCATTTCTGCATCTGCTCCGTCAAGAAGTCTGGCTTGTTCGCCGCCGTTTGTTATATTCTTGATAAGCATCAGATCGAGGTTGTTTCGGCTCATGTCTCCTCGGATTTCCATCGCACGTTCTTTATGATTCAAGGCCATGTGAATGGAAGTTGGGACGAATTCGGGGCAGCGATGGTGGCAGAAGAAATGTTCAAGCAAGCTGCGGGGGAGATCAACTTCTGGTTGTCTTCTTGA

Protein sequence

MAAPHNIWAFLNSGSDVDIDIDLDDDNDNDNSNDKSPANTDLLPPLPLSGRKREAAEGRPGGGGESTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQTLQTLYNQKLEKLQNNNPAVSYKAAHYHPQQQQQQQQFSSTTTTHQAFLADQASSSNGMSSTLLPPPLPPLPPLPPPFSDTTTTFQTWTSSHLVLCICGGEAHFCICSVKKSGLFAAVCYILDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS

Homology

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BLAST of Carg26411 vs. NCBI nr
Match: XP_022927859.1 (transcription factor bHLH95-like [Cucurbita moschata])

HSP 1 Score: 380.6 bits (976), Expect = 5.0e-102
Identity = 286/298 (95.97%), Postives = 286/298 (95.97%), Query Frame = 0


Query: 1   MAAPHNIWAFLNSGXXXXXXXXXXXXXXXXXXXXXXXXXXXLLPPLPLSGRKREAAEGRP 60
           MAAPHNIWAFLNSGXXXXXXXXXXXXXXXXXXXXXXXX   LLPPLPLSGRKREA EGRP
Sbjct: 1   MAAPHNIWAFLNSGXXXXXXXXXXXXXXXXXXXXXXXXNTDLLPPLPLSGRKREAVEGRP 60

Query: 61  GGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT 120
           GG   STEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT
Sbjct: 61  GGGGESTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT 120

Query: 121 LQTLYNQKLEKLQNNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           LQTLYNQKLEKLQNNNPAVSY   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 121 LQTLYNQKLEKLQNNNPAVSY---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICSVKKSGLFAAVCYI 240
           XXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVL ICGGEAHFCICSVKKSGLFAAVCYI
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLSICGGEAHFCICSVKKSGLFAAVCYI 240

Query: 241 LDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS 299
           LDKHQIEVVSAHVSSDFHRTFFMIQGHVNG WDEFGAAMVAEEMFKQAAGEINFWLSS
Sbjct: 241 LDKHQIEVVSAHVSSDFHRTFFMIQGHVNGRWDEFGAAMVAEEMFKQAAGEINFWLSS 295

[Close]

BLAST of Carg26411 vs. NCBI nr
Match: XP_023531698.1 (transcription factor bHLH95-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 373.6 bits (958), Expect = 6.1e-100
Identity = 281/298 (94.30%), Postives = 283/298 (94.97%), Query Frame = 0


Query: 1   MAAPHNIWAFLNSGXXXXXXXXXXXXXXXXXXXXXXXXXXXLLPPLPLSGRKREAAEGRP 60
           M+APHNIWAFLNS  XXXXXXXXXXXXXXXXXXXXXXX   LLPPLPLSGRKREA EGRP
Sbjct: 1   MSAPHNIWAFLNSASXXXXXXXXXXXXXXXXXXXXXXXNTDLLPPLPLSGRKREAVEGRP 60

Query: 61  GGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT 120
           GG   STEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT
Sbjct: 61  GGGGESTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT 120

Query: 121 LQTLYNQKLEKLQNNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           LQTLYNQKLEKLQNNNPAVSY    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 121 LQTLYNQKLEKLQNNNPAVSY--KAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICSVKKSGLFAAVCYI 240
           XXXXXXXXXXXXXXXXXXXXXXX QTWTSS+LVL ICGGEAHFCICSVKKSGLFAAVCYI
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXQTWTSSNLVLSICGGEAHFCICSVKKSGLFAAVCYI 240

Query: 241 LDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS 299
           LDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS
Sbjct: 241 LDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS 296

[Close]

BLAST of Carg26411 vs. NCBI nr
Match: XP_022988868.1 (transcription factor bHLH95-like [Cucurbita maxima])

HSP 1 Score: 356.3 bits (913), Expect = 1.0e-94
Identity = 265/298 (88.93%), Postives = 269/298 (90.27%), Query Frame = 0


Query: 1   MAAPHNIWAFLNSGXXXXXXXXXXXXXXXXXXXXXXXXXXXLLPPLPLSGRKREAAEGRP 60
           MAAPHNIWAFLNS XXXXXXXXXXXXXXXXXXXXXX     LLPPLPLSGRKREA EGRP
Sbjct: 1   MAAPHNIWAFLNSXXXXXXXXXXXXXXXXXXXXXXXPANTDLLPPLPLSGRKREAVEGRP 60

Query: 61  GGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT 120
           G    STEHEIHIWTERERRK+MRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT
Sbjct: 61  GRGVESTEHEIHIWTERERRKKMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQT 120

Query: 121 LQTLYNQKLEKLQNNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           LQ LY+QKLEKLQNNNPA SY          XXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 121 LQKLYSQKLEKLQNNNPAGSY-------KAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICSVKKSGLFAAVCYI 240
           XXXXXXXXXXXXXXXXXXXXXX FQTWTSS+LVL ICGGEAHFCICSVKK GLFAAVCYI
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXAFQTWTSSNLVLSICGGEAHFCICSVKKPGLFAAVCYI 240

Query: 241 LDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS 299
           +DKHQIEVVSAHVSSDFHR F MIQ HVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS
Sbjct: 241 IDKHQIEVVSAHVSSDFHRMFIMIQAHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS 291

[Close]

BLAST of Carg26411 vs. NCBI nr
Match: XP_004137111.2 (PREDICTED: transcription factor bHLH95 [Cucumis sativus])

HSP 1 Score: 246.5 bits (628), Expect = 1.1e-61
Identity = 131/251 (52.19%), Postives = 159/251 (63.35%), Query Frame = 0


Query: 49  SGRKRE-AAEGRPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIV 108
           +G+KRE   EG+  G   S++H+IHIWTERERRK+MRNMFSNLHAL+PHLPPKADKS+IV
Sbjct: 3   AGKKRELETEGKSAGATESSDHDIHIWTERERRKKMRNMFSNLHALLPHLPPKADKSSIV 62

Query: 109 DEAVNYIKTVQQTLQTLYNQKLEKLQNNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXX 168
           DEAVNYIKT+Q+T Q L+ QK+EKL         XX                        
Sbjct: 63  DEAVNYIKTLQETFQRLHKQKVEKLXXXXXXXXXXXMGCSKIVHHPLPTTREAFLADQAS 122

Query: 169 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICS 228
                                               FQTWTSS+LVL +CG  AHFC+CS
Sbjct: 123 SNDDMSSSATLLSHLSTTITPENVLSNLPLPSPPSAFQTWTSSNLVLSVCGRHAHFCVCS 182

Query: 229 VKKSGLFAAVCYILDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQ 288
            KK GLFAA+CY+LDK++I+VVSAHVSSD H++FFMIQ HV+  ++EFGAA VAE+ FK+
Sbjct: 183 AKKPGLFAALCYVLDKYRIDVVSAHVSSDVHQSFFMIQAHVSRGYNEFGAATVAEDTFKE 242

Query: 289 AAGEINFWLSS 299
           AAGEI FWLSS
Sbjct: 243 AAGEIIFWLSS 253

[Close]

BLAST of Carg26411 vs. NCBI nr
Match: XP_022972450.1 (transcription factor bHLH95-like [Cucurbita maxima])

HSP 1 Score: 243.0 bits (619), Expect = 1.2e-60
Identity = 132/256 (51.56%), Postives = 160/256 (62.50%), Query Frame = 0


Query: 44  PPLPLSGRKREAAEGRPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADK 103
           PP   SG+KR+AAE +  G   S  H+IHIWTERERRK+MR MF+NLHAL+PHLP K DK
Sbjct: 23  PPPTQSGKKRDAAERKSCGGAASA-HDIHIWTERERRKKMRTMFANLHALLPHLPAKVDK 82

Query: 104 STIVDEAVNYIKTVQQTLQTLYNQKLEKLQNN-NPAVSYXXXXXXXXXXXXXXXXXXXXX 163
           S++VDEAVN+IKT+QQTLQ L+ QKLE+L N+ NP++S+                     
Sbjct: 83  SSLVDEAVNHIKTLQQTLQNLHKQKLERLNNDTNPSLSF---SKIVHHPSARSSAATTRE 142

Query: 164 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAH 223
                                                    FQTW SS+LVL +CG  AH
Sbjct: 143 AFLANQASSTNDMSSTLFPQLSTAAAAGDNVVSILPSPPSSFQTWLSSNLVLNVCGHHAH 202

Query: 224 FCICSVKKSGLFAAVCYILDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAE 283
           FCICSVKK GLF  +CY+L+KHQI VVSAHVSSD+HR+FFMI  H+NG +DEF AA VAE
Sbjct: 203 FCICSVKKPGLFPTLCYVLEKHQIHVVSAHVSSDYHRSFFMIHAHMNGGYDEFEAATVAE 262

Query: 284 EMFKQAAGEINFWLSS 299
           + FK+AA EI FWLSS
Sbjct: 263 DTFKEAAREIIFWLSS 274

[Close]

BLAST of Carg26411 vs. TAIR10
Match: AT1G49770.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 73.9 bits (180), Expect = 1.8e-13
Identity = 39/98 (39.80%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 204 FQTWTSSHLVLCICGGEAHFCICSVK-KSGLFAAVCYILDKHQIEVVSAHVSSDFHRTFF 263
           F TW+S ++VL ICG EA F +C  K K G+F +VCY+ +K+ +EV+ A+VSS+   + +
Sbjct: 210 FDTWSSRNVVLTICGNEAFFNLCVPKHKPGVFTSVCYLFEKYNMEVLFANVSSNVFWSTY 269

Query: 264 MIQGHVNGSWDE--FGAAMVAEEMFKQAAGEINFWLSS 299
           +IQ  VN S +    G  +   ++FKQ + E+  + SS
Sbjct: 270 VIQAQVNPSCENQLLGNGLGVVDVFKQVSQELVLYFSS 307

[Close]

BLAST of Carg26411 vs. TAIR10
Match: AT3G61950.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 50.4 bits (119), Expect = 2.2e-06
Identity = 26/69 (37.68%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 72  HIWTERERRKRMRNMFSNLHALI-PHLPPKADKSTIVDEAVNYIKTVQQTLQTLYNQKLE 131
           HI  ER RR++M    ++L AL+ P    + D+++IV  A+NY+K ++Q +Q+L +QK  
Sbjct: 180 HIAVERNRRRQMNEHINSLRALLPPSYIQRGDQASIVGGAINYVKVLEQIIQSLESQKRT 239

Query: 132 KLQNNNPAV 140
           + Q+N+  V
Sbjct: 240 QQQSNSEVV 248

[Close]

BLAST of Carg26411 vs. TAIR10
Match: AT2G43010.1 (phytochrome interacting factor 4)

HSP 1 Score: 48.5 bits (114), Expect = 8.2e-06
Identity = 25/60 (41.67%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 59  RPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQ 118
           R G    S   E+H  +ER RR R+      L  LIPH   K DK++I+DEA++Y+K++Q
Sbjct: 249 RSGSNRRSRAAEVHNLSERRRRDRINERMKALQELIPHC-SKTDKASILDEAIDYLKSLQ 307

[Close]

BLAST of Carg26411 vs. TAIR10
Match: AT2G22760.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-05
Identity = 25/57 (43.86%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 72  HIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQTLQTLYNQK 129
           H+  ER+RR+++   F  L AL+P L  KADK TI+D+A++ +K +Q+ L+TL  +K
Sbjct: 120 HVLAERKRREKLSEKFIALSALLPGL-KKADKVTILDDAISRMKQLQEQLRTLKEEK 175

[Close]

BLAST of Carg26411 vs. TAIR10
Match: AT3G59060.2 (phytochrome interacting factor 3-like 6)

HSP 1 Score: 47.4 bits (111), Expect = 1.8e-05
Identity = 24/60 (40.00%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 59  RPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQ 118
           R G    S   E+H  +ER RR R+      L  LIPH   + DK++I+DEA++Y+K++Q
Sbjct: 248 RSGSTRRSRAAEVHNLSERRRRDRINERMKALQELIPHC-SRTDKASILDEAIDYLKSLQ 306

[Close]

BLAST of Carg26411 vs. Swiss-Prot
Match: sp|Q9FXA3|BH095_ARATH (Transcription factor bHLH95 OS=Arabidopsis thaliana OX=3702 GN=BHLH95 PE=2 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 3.3e-12
Identity = 39/98 (39.80%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 204 FQTWTSSHLVLCICGGEAHFCICSVK-KSGLFAAVCYILDKHQIEVVSAHVSSDFHRTFF 263
           F TW+S ++VL ICG EA F +C  K K G+F +VCY+ +K+ +EV+ A+VSS+   + +
Sbjct: 210 FDTWSSRNVVLTICGNEAFFNLCVPKHKPGVFTSVCYLFEKYNMEVLFANVSSNVFWSTY 269

Query: 264 MIQGHVNGSWDE--FGAAMVAEEMFKQAAGEINFWLSS 299
           +IQ  VN S +    G  +   ++FKQ + E+  + SS
Sbjct: 270 VIQAQVNPSCENQLLGNGLGVVDVFKQVSQELVLYFSS 307

[Close]

BLAST of Carg26411 vs. Swiss-Prot
Match: sp|Q700E4|BH067_ARATH (Transcription factor bHLH67 OS=Arabidopsis thaliana OX=3702 GN=BHLH67 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.9e-05
Identity = 26/69 (37.68%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 72  HIWTERERRKRMRNMFSNLHALI-PHLPPKADKSTIVDEAVNYIKTVQQTLQTLYNQKLE 131
           HI  ER RR++M    ++L AL+ P    + D+++IV  A+NY+K ++Q +Q+L +QK  
Sbjct: 180 HIAVERNRRRQMNEHINSLRALLPPSYIQRGDQASIVGGAINYVKVLEQIIQSLESQKRT 239

Query: 132 KLQNNNPAV 140
           + Q+N+  V
Sbjct: 240 QQQSNSEVV 248

[Close]

BLAST of Carg26411 vs. Swiss-Prot
Match: sp|Q8W2F3|PIF4_ARATH (Transcription factor PIF4 OS=Arabidopsis thaliana OX=3702 GN=PIF4 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.5e-04
Identity = 25/60 (41.67%), Postives = 36/60 (60.00%), Query Frame = 0


Query: 59  RPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQ 118
           R G    S   E+H  +ER RR R+      L  LIPH   K DK++I+DEA++Y+K++Q
Sbjct: 249 RSGSNRRSRAAEVHNLSERRRRDRINERMKALQELIPHC-SKTDKASILDEAIDYLKSLQ 307

[Close]

BLAST of Carg26411 vs. Swiss-Prot
Match: sp|Q10CH5|PIL13_ORYSJ (Transcription factor PHYTOCHROME INTERACTING FACTOR-LIKE 13 OS=Oryza sativa subsp. japonica OX=39947 GN=PIL13 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.5e-04
Identity = 25/72 (34.72%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 54  EAAEGRPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNY 113
           +++  R G    +   E+H  +ER RR R+      L  LIPH   K DK++I+DEA+ Y
Sbjct: 207 KSSSRRYGSKRRTRAAEVHNLSERRRRDRINEKMRALQELIPHC-NKTDKASILDEAIEY 266

Query: 114 IKTVQQTLQTLY 126
           +K++Q  +Q ++
Sbjct: 267 LKSLQMQVQIMW 277

[Close]

BLAST of Carg26411 vs. Swiss-Prot
Match: sp|Q1PF16|BH019_ARATH (Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.9e-04
Identity = 25/57 (43.86%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 72  HIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQTLQTLYNQK 129
           H+  ER+RR+++   F  L AL+P L  KADK TI+D+A++ +K +Q+ L+TL  +K
Sbjct: 120 HVLAERKRREKLSEKFIALSALLPGL-KKADKVTILDDAISRMKQLQEQLRTLKEEK 175

[Close]

BLAST of Carg26411 vs. TrEMBL
Match: tr|A0A0A0K4R5|A0A0A0K4R5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073650 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.4e-60
Identity = 127/242 (52.48%), Postives = 153/242 (63.22%), Query Frame = 0


Query: 57  EGRPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKT 116
           EG+  G   S++H+IHIWTERERRK+MRNMFSNLHAL+PHLPPKADKS+IVDEAVNYIKT
Sbjct: 56  EGKSAGATESSDHDIHIWTERERRKKMRNMFSNLHALLPHLPPKADKSSIVDEAVNYIKT 115

Query: 117 VQQTLQTLYNQKLEKLQNNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 176
           +Q+T Q L+ QK+EKL         XX                                 
Sbjct: 116 LQETFQRLHKQKVEKLXXXXXXXXXXXMGCSKIVHHPLPTTREAFLADQASSNDDMSSSA 175

Query: 177 XXXXXXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICSVKKSGLFAA 236
                                      FQTWTSS+LVL +CG  AHFC+CS KK GLFAA
Sbjct: 176 TLLSHLSTTITPENVLSNLPLPSPPSAFQTWTSSNLVLSVCGRHAHFCVCSAKKPGLFAA 235

Query: 237 VCYILDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWL 296
           +CY+LDK++I+VVSAHVSSD H++FFMIQ HV+  ++EFGAA VAE+ FK+AAGEI FWL
Sbjct: 236 LCYVLDKYRIDVVSAHVSSDVHQSFFMIQAHVSRGYNEFGAATVAEDTFKEAAGEIIFWL 295

Query: 297 SS 299
           SS
Sbjct: 296 SS 297

[Close]

BLAST of Carg26411 vs. TrEMBL
Match: tr|A0A1S4E152|A0A1S4E152_CUCME (transcription factor bHLH95-like OS=Cucumis melo OX=3656 GN=LOC103495238 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 4.1e-52
Identity = 154/223 (69.06%), Postives = 179/223 (80.27%), Query Frame = 0


Query: 50  GRKR--EAAEGRPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIV 109
           G+KR  E  EG+  G   S++H+IHIWTERERRK+MRNMFSNLHAL+PHLPPKADKS+IV
Sbjct: 50  GKKRELETHEGKSAGATESSDHDIHIWTERERRKKMRNMFSNLHALLPHLPPKADKSSIV 109

Query: 110 DEAVNYIKTVQQTLQTLYNQKLEKLQNN-NPAVSYXXXXXXXXXXXXXXXXXXXXXXXXX 169
           DEAVNYIKT+Q+T + L+ QK+EKL NN NP++S                       XXX
Sbjct: 110 DEAVNYIKTLQETFERLHKQKVEKLNNNSNPSMS-CSKIVHNPALPTTREAFLADQAXXX 169

Query: 170 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCIC 229
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQTWTSS+LVL +CG  AHFC+C
Sbjct: 170 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQTWTSSNLVLSVCGRHAHFCVC 229

Query: 230 SVKKSGLFAAVCYILDKHQIEVVSAHVSSDFHRTFFMIQGHVN 270
           S KK GLFAA+CY+LDKH+I+VVSAHVSSD H++FFMIQ HV+
Sbjct: 230 SAKKPGLFAALCYVLDKHRIDVVSAHVSSDLHQSFFMIQAHVS 271

[Close]

BLAST of Carg26411 vs. TrEMBL
Match: tr|A0A2H5PRD2|A0A2H5PRD2_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_160480 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 2.3e-50
Identity = 112/232 (48.28%), Postives = 138/232 (59.48%), Query Frame = 0


Query: 67  TEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQTLQTLYN 126
           +EHEIHIWTERERRK+MRNMF+NLH+L+P LPPKADKS+IVDEAV+YIKT+QQTL+ L  
Sbjct: 98  SEHEIHIWTERERRKKMRNMFANLHSLLPQLPPKADKSSIVDEAVSYIKTLQQTLRKLQK 157

Query: 127 QKLEKLQNNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 186
           QKLE+LQ                                                     
Sbjct: 158 QKLERLQG-----------VASFGFEASAAITPQNKLAIVAHDHQQGSSSNLLGANSTDA 217

Query: 187 XXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICSVKKSGLFAAVCYILDKHQI 246
                            FQTWTSS++VL ICG EAH  ICS KK G+F+ +CY+L+KH+I
Sbjct: 218 TNNSSNLLSVSPTYPVIFQTWTSSNVVLNICGDEAHISICSPKKPGMFSTICYVLEKHKI 277

Query: 247 EVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS 299
           EV+SA VSSD  R  +MIQ HVNG+ D+F  A+  EEM+KQAA E+ FW+SS
Sbjct: 278 EVISAQVSSDLTRRMYMIQVHVNGASDQFSEALPVEEMYKQAASELMFWISS 318

[Close]

BLAST of Carg26411 vs. TrEMBL
Match: tr|V4UAF0|V4UAF0_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10030106mg PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.8e-50
Identity = 111/232 (47.84%), Postives = 138/232 (59.48%), Query Frame = 0


Query: 67  TEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVNYIKTVQQTLQTLYN 126
           +EHEIHIWTERERRK+MRNMF+NLH+L+P LPPKADKS+IVDEAV+YIKT+QQTL+ L  
Sbjct: 98  SEHEIHIWTERERRKKMRNMFANLHSLLPQLPPKADKSSIVDEAVSYIKTLQQTLRKLQK 157

Query: 127 QKLEKLQNNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 186
           QKLE+LQ                                                     
Sbjct: 158 QKLERLQG-----------VASFGFEASAAITPQNKLAIVAHDRQQESSSNLLGANSTDA 217

Query: 187 XXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICSVKKSGLFAAVCYILDKHQI 246
                            FQTW+SS++VL ICG EAH  ICS KK G+F+ +CY+L+KH+I
Sbjct: 218 TNNSSNLLSVSPTYPVIFQTWSSSNVVLNICGDEAHISICSPKKPGMFSTICYLLEKHKI 277

Query: 247 EVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQAAGEINFWLSS 299
           EV+SA VSSD  R  +MIQ HVNG+ D+F  A+  EEM+KQAA E+ FW+SS
Sbjct: 278 EVISAQVSSDLTRRMYMIQAHVNGASDQFSEALPVEEMYKQAASELMFWISS 318

[Close]

BLAST of Carg26411 vs. TrEMBL
Match: tr|A0A1J6INZ6|A0A1J6INZ6_NICAT (Transcription factor bhlh95 OS=Nicotiana attenuata OX=49451 GN=BHLH95_1 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 5.2e-47
Identity = 110/250 (44.00%), Postives = 142/250 (56.80%), Query Frame = 0


Query: 53  REAAEGRPGGXXXSTEHEIHIWTERERRKRMRNMFSNLHALIPHLPPKADKSTIVDEAVN 112
           RE  EG+  G   S +HE+HIWTERERRK+MRNMFSNLHAL+P LPPKADKSTIVDEAVN
Sbjct: 75  REVNEGKVNGAGES-DHEMHIWTERERRKKMRNMFSNLHALLPQLPPKADKSTIVDEAVN 134

Query: 113 YIKTVQQTLQTLYNQKLEKLQ----NNNPAVSYXXXXXXXXXXXXXXXXXXXXXXXXXXX 172
           YIKT+Q TLQ L  QKLE+L     + NP++                             
Sbjct: 135 YIKTLQHTLQKLQRQKLERLHGMATSMNPSI-----------------ITSQKLSADSII 194

Query: 173 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQTWTSSHLVLCICGGEAHFCICSV 232
                                              FQ+WTS +++L +CG EAH  +C  
Sbjct: 195 SREAFLADQGSTNNAAISPTNNAANPISMPHFPAVFQSWTSPNVILNVCGEEAHISVCCP 254

Query: 233 KKSGLFAAVCYILDKHQIEVVSAHVSSDFHRTFFMIQGHVNGSWDEFGAAMVAEEMFKQA 292
           KK GL +++CY+L+KH+IEVVSA +SS+ HR  +MIQ H +G+ D+F      EE++KQA
Sbjct: 255 KKPGLLSSICYVLEKHKIEVVSAQISSNDHRNMYMIQAHASGASDKFSETFPVEEIYKQA 306

Query: 293 AGEINFWLSS 299
           AGEI   ++S
Sbjct: 315 AGEIIMCVTS 306

The following BLAST results are available for this feature:

BLAST of Carg26411 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927859.1	5.0e-102	95.97	transcription factor bHLH95-like [Cucurbita moschata]	[more]
XP_023531698.1	6.1e-100	94.30	transcription factor bHLH95-like [Cucurbita pepo subsp. pepo]	[more]
XP_022988868.1	1.0e-94	88.93	transcription factor bHLH95-like [Cucurbita maxima]	[more]
XP_004137111.2	1.1e-61	52.19	PREDICTED: transcription factor bHLH95 [Cucumis sativus]	[more]
XP_022972450.1	1.2e-60	51.56	transcription factor bHLH95-like [Cucurbita maxima]	[more]

BLAST of Carg26411 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G49770.1	1.8e-13	39.80	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT3G61950.1	2.2e-06	37.68	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G43010.1	8.2e-06	41.67	phytochrome interacting factor 4	[more]
AT2G22760.1	1.1e-05	43.86	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT3G59060.2	1.8e-05	40.00	phytochrome interacting factor 3-like 6	[more]

BLAST of Carg26411 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FXA3\|BH095_ARATH	3.3e-12	39.80	Transcription factor bHLH95 OS=Arabidopsis thaliana OX=3702 GN=BHLH95 PE=2 SV=2	[more]
sp\|Q700E4\|BH067_ARATH	3.9e-05	37.68	Transcription factor bHLH67 OS=Arabidopsis thaliana OX=3702 GN=BHLH67 PE=2 SV=1	[more]
sp\|Q8W2F3\|PIF4_ARATH	1.5e-04	41.67	Transcription factor PIF4 OS=Arabidopsis thaliana OX=3702 GN=PIF4 PE=1 SV=1	[more]
sp\|Q10CH5\|PIL13_ORYSJ	1.5e-04	34.72	Transcription factor PHYTOCHROME INTERACTING FACTOR-LIKE 13 OS=Oryza sativa subs...	[more]
sp\|Q1PF16\|BH019_ARATH	1.9e-04	43.86	Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1	[more]

BLAST of Carg26411 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0K4R5\|A0A0A0K4R5_CUCSA	1.4e-60	52.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073650 PE=4 SV=1	[more]
tr\|A0A1S4E152\|A0A1S4E152_CUCME	4.1e-52	69.06	transcription factor bHLH95-like OS=Cucumis melo OX=3656 GN=LOC103495238 PE=4 SV...	[more]
tr\|A0A2H5PRD2\|A0A2H5PRD2_CITUN	2.3e-50	48.28	Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_160480 PE=4 SV=1	[more]
tr\|V4UAF0\|V4UAF0_9ROSI	3.8e-50	47.84	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10030106mg PE=4 ...	[more]
tr\|A0A1J6INZ6\|A0A1J6INZ6_NICAT	5.2e-47	44.00	Transcription factor bhlh95 OS=Nicotiana attenuata OX=49451 GN=BHLH95_1 PE=4 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0046983	protein dimerization activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036638	HLH_DNA-bd_sf
IPR011598	bHLH_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046983	protein dimerization activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26411-RA	Carg26411-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 75..123 e-value: 2.4E-8 score: 43.7
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 75..118 e-value: 6.5E-10 score: 38.7
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 67..117 score: 13.173
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	CDD	cd00083	HLH	coord: 72..122 e-value: 3.22278E-13 score: 62.2315
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	G3DSA:4.10.280.10		coord: 64..136 e-value: 4.3E-15 score: 57.4
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	SSF47459	HLH, helix-loop-helix DNA-binding domain	coord: 68..128
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 142..194
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 142..181
None	No IPR available	PANTHER	PTHR11514	MYC	coord: 55..297
None	No IPR available	PANTHER	PTHR11514:SF96	SUBFAMILY NOT NAMED	coord: 55..297

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg26411	Cla016013	Watermelon (97103) v1	carwmB0799
Carg26411	Csa7G073650	Cucumber (Chinese Long) v2	carcuB0817
Carg26411	MELO3C018451	Melon (DHL92) v3.5.1	carmeB0798
Carg26411	ClCG02G005950	Watermelon (Charleston Gray)	carwcgB0774
Carg26411	CSPI07G07620	Wild cucumber (PI 183967)	carcpiB0837
Carg26411	Cucsa.219850	Cucumber (Gy14) v1	carcgyB0914
Carg26411	CmaCh19G006030	Cucurbita maxima (Rimu)	carcmaB1114
Carg26411	CmaCh11G017490	Cucurbita maxima (Rimu)	carcmaB1113
Carg26411	CmoCh19G006210	Cucurbita moschata (Rifu)	carcmoB1086
Carg26411	CmoCh11G013630	Cucurbita moschata (Rifu)	carcmoB1085
Carg26411	Lsi03G004950	Bottle gourd (USVL1VR-Ls)	carlsiB736
Carg26411	Cp4.1LG15g04870	Cucurbita pepo (Zucchini)	carcpeB1125
Carg26411	Cp4.1LG04g08060	Cucurbita pepo (Zucchini)	carcpeB1126
Carg26411	CsGy7G007190	Cucumber (Gy14) v2	carcgybB0791
Carg26411	MELO3C018451.2	Melon (DHL92) v3.6.1	carmedB0810
Carg26411	CsaV3_7G008580	Cucumber (Chinese Long) v3	carcucB0841
Carg26411	Cla97C02G032920	Watermelon (97103) v2	carwmbB0838
Carg26411	Bhi05G000921	Wax gourd	carwgoB0937

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg26411	Carg22068	Silver-seed gourd	carcarB246

The following block(s) are covering this gene:


Gene	Organism	Block
Carg26411	Bottle gourd (USVL1VR-Ls)	carlsiB737
Carg26411	Watermelon (Charleston Gray)	carwcgB0775