Carg26127 (gene) Silver-seed gourd

NameCarg26127
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionE3 ubiquitin-protein ligase RING1-like
LocationCucurbita_argyrosperma_scaffold_220 : 326791 .. 327711 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAATGGTCGGAACACCCATTGGTGTTATAGATGCAGAAGTTCGGTACGTCCTCGAGGTAGAGATGCAGTTTGCCCCAACTGCAATGGAGGATTTATCCACGAACTCGATGATATGGTGCATGGAAACCCATTTGAATTCTTTGGTTTGGATGATGATGATGAAGTTCACAATCATGGTATGGGAGCATTCTCTGCTTTTATGAGACAGCGGTTAGCTGAAAGAAACGACATGAGGGGAAGATCAGAGTCGGTTTTCGAGCTTAGCCCGGGATTTGGACCGCTATTAGTATTTGGTGGTCAAGTTCCAATGAGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCTCCTGGCGTTGGCCTTACACGAGGCAATAGCGGCGACTACTTTATAGGCCCTGGACTGGAGGAGCTATTTGAACAGCTTTCAGGAAATGGTCATCGCCGCCCTCCGCCAGCATCTAGATCATCAATTGATGCAATACCAACCGTTAGGATCACGCAACAACATCTTCACTCGGATTCACATTGTCCAGTTTGCAAAGAAAAATTTGAACTCGAGTCCAAAGCTAGGCAGATGCCCTGTAGTCACATGTACCACTCAGATTGTATTGTTCCATGGCTAGTCCAACACAACTCTTGCCCTGTCTGTCGCCAGGAACTGCCACCACAGGGAGTAGGGGTCGGGGGCGGGGGCGGAGGCGGAGGCGGGGGACATATCGCCAATGGTCAAAACGAAAGCAGCAGCTACAACAACGGTAATGGGTCCAGTGTGAATCCAGGCAGACGCAATCCATTCTCTAATCTGTGGCCATTTAGGTCGTCTAGTTCAAGGACCAACCATGGCGGAGCAGGGAGTAGTGAAGCGACGAACCACCAGATGGGGTATTCGGGATATCGATACACTAATTCACCATGA

mRNA sequence

ATGTCGAATGGTCGGAACACCCATTGGTGTTATAGATGCAGAAGTTCGGTACGTCCTCGAGGTAGAGATGCAGTTTGCCCCAACTGCAATGGAGGATTTATCCACGAACTCGATGATATGGTGCATGGAAACCCATTTGAATTCTTTGGTTTGGATGATGATGATGAAGTTCACAATCATGGTATGGGAGCATTCTCTGCTTTTATGAGACAGCGGTTAGCTGAAAGAAACGACATGAGGGGAAGATCAGAGTCGGTTTTCGAGCTTAGCCCGGGATTTGGACCGCTATTAGTATTTGGTGGTCAAGTTCCAATGAGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCTCCTGGCGTTGGCCTTACACGAGGCAATAGCGGCGACTACTTTATAGGCCCTGGACTGGAGGAGCTATTTGAACAGCTTTCAGGAAATGGTCATCGCCGCCCTCCGCCAGCATCTAGATCATCAATTGATGCAATACCAACCGTTAGGATCACGCAACAACATCTTCACTCGGATTCACATTGTCCAGTTTGCAAAGAAAAATTTGAACTCGAGTCCAAAGCTAGGCAGATGCCCTGTAGTCACATGTACCACTCAGATTGTATTGTTCCATGGCTAGTCCAACACAACTCTTGCCCTGTCTGTCGCCAGGAACTGCCACCACAGGGAGTAGGGGTCGGGGGCGGGGGCGGAGGCGGAGGCGGGGGACATATCGCCAATGGTCAAAACGAAAGCAGCAGCTACAACAACGGTAATGGGTCCAGTGTGAATCCAGGCAGACGCAATCCATTCTCTAATCTGTGGCCATTTAGGTCGTCTAGTTCAAGGACCAACCATGGCGGAGCAGGGAGTAGTGAAGCGACGAACCACCAGATGGGGTATTCGGGATATCGATACACTAATTCACCATGA

Coding sequence (CDS)

ATGTCGAATGGTCGGAACACCCATTGGTGTTATAGATGCAGAAGTTCGGTACGTCCTCGAGGTAGAGATGCAGTTTGCCCCAACTGCAATGGAGGATTTATCCACGAACTCGATGATATGGTGCATGGAAACCCATTTGAATTCTTTGGTTTGGATGATGATGATGAAGTTCACAATCATGGTATGGGAGCATTCTCTGCTTTTATGAGACAGCGGTTAGCTGAAAGAAACGACATGAGGGGAAGATCAGAGTCGGTTTTCGAGCTTAGCCCGGGATTTGGACCGCTATTAGTATTTGGTGGTCAAGTTCCAATGAGAAACAGCAGGTTAGAAGCTCTCTTCAATGGGGCTCCTGGCGTTGGCCTTACACGAGGCAATAGCGGCGACTACTTTATAGGCCCTGGACTGGAGGAGCTATTTGAACAGCTTTCAGGAAATGGTCATCGCCGCCCTCCGCCAGCATCTAGATCATCAATTGATGCAATACCAACCGTTAGGATCACGCAACAACATCTTCACTCGGATTCACATTGTCCAGTTTGCAAAGAAAAATTTGAACTCGAGTCCAAAGCTAGGCAGATGCCCTGTAGTCACATGTACCACTCAGATTGTATTGTTCCATGGCTAGTCCAACACAACTCTTGCCCTGTCTGTCGCCAGGAACTGCCACCACAGGGAGTAGGGGTCGGGGGCGGGGGCGGAGGCGGAGGCGGGGGACATATCGCCAATGGTCAAAACGAAAGCAGCAGCTACAACAACGGTAATGGGTCCAGTGTGAATCCAGGCAGACGCAATCCATTCTCTAATCTGTGGCCATTTAGGTCGTCTAGTTCAAGGACCAACCATGGCGGAGCAGGGAGTAGTGAAGCGACGAACCACCAGATGGGGTATTCGGGATATCGATACACTAATTCACCATGA

Protein sequence

MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNHGMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGVGLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVGVGGGGGGGGGGHIANGQNESSSYNNGNGSSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGYRYTNSP
BLAST of Carg26127 vs. NCBI nr
Match: XP_022950677.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita moschata] >XP_022950678.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita moschata])

HSP 1 Score: 564.3 bits (1453), Expect = 2.5e-157
Identity = 297/306 (97.06%), Postives = 299/306 (97.71%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH
Sbjct: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60

Query: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120
           GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV
Sbjct: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120

Query: 121 GLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPV 180
           GLTRGNSGDYFIGPGLEELFEQ+SGNGHR PPPASRSSIDAIPTVRI QQHL SDSHCPV
Sbjct: 121 GLTRGNSGDYFIGPGLEELFEQISGNGHRGPPPASRSSIDAIPTVRIAQQHLRSDSHCPV 180

Query: 181 CKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240
           CKEKFELES+ARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGV    XXXXXXXXX
Sbjct: 181 CKEKFELESEARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGV----XXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300
           XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY
Sbjct: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300

Query: 301 RYTNSP 307
           RYTNSP
Sbjct: 301 RYTNSP 302

BLAST of Carg26127 vs. NCBI nr
Match: XP_023543475.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023543476.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023543477.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.1 bits (1437), Expect = 1.8e-155
Identity = 290/306 (94.77%), Postives = 295/306 (96.41%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH
Sbjct: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60

Query: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120
           GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV
Sbjct: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120

Query: 121 GLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPV 180
           GLTRGNSGDY IGPGLEELFEQLSGNGHR PPPASRSSIDAIPTVRI QQHL SDSHCPV
Sbjct: 121 GLTRGNSGDYLIGPGLEELFEQLSGNGHRGPPPASRSSIDAIPTVRIAQQHLRSDSHCPV 180

Query: 181 CKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240
           CKEKFEL S+ARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX
Sbjct: 181 CKEKFELGSEARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300
           XXXXXXXXXXXX     S+NPGRRNPFSNLWPFRSS+SR+NHGGAGSS+ TNHQMGYSGY
Sbjct: 241 XXXXXXXXXXXXSGNGSSINPGRRNPFSNLWPFRSSNSRSNHGGAGSSDPTNHQMGYSGY 300

Query: 301 RYTNSP 307
           RYTNSP
Sbjct: 301 RYTNSP 306

BLAST of Carg26127 vs. NCBI nr
Match: XP_022967862.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita maxima] >XP_022967863.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita maxima])

HSP 1 Score: 547.0 bits (1408), Expect = 4.2e-152
Identity = 275/306 (89.87%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH
Sbjct: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60

Query: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120
           GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV
Sbjct: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120

Query: 121 GLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPV 180
           GLTRGNSG+YFIGPGLEELFEQLS NGHR PPPASRSSIDAIPTV I QQHL SDSHCPV
Sbjct: 121 GLTRGNSGNYFIGPGLEELFEQLSANGHRGPPPASRSSIDAIPTVGIAQQHLRSDSHCPV 180

Query: 181 CKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240
           CKEKFEL S+ARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQG    XXXXXXXXXX
Sbjct: 181 CKEKFELGSEARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQG----XXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300
           X                SVN GRRNPFSNLWPFRSSSSR+NHGGAG+SEATNHQMGYSGY
Sbjct: 241 XANGQNESSSYNSGNGSSVNLGRRNPFSNLWPFRSSSSRSNHGGAGTSEATNHQMGYSGY 300

Query: 301 RYTNSP 307
           RYTNSP
Sbjct: 301 RYTNSP 302

BLAST of Carg26127 vs. NCBI nr
Match: XP_004144313.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus] >XP_004144314.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus] >XP_011653844.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus] >XP_011653845.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus] >KGN54669.1 hypothetical protein Csa_4G419610 [Cucumis sativus])

HSP 1 Score: 494.6 bits (1272), Expect = 2.4e-136
Identity = 257/306 (83.99%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MS GRNTHWCYRCR SVR RGRDAVCPNCNGGFI ELDDMV GNPF+FFGLD D++  +H
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDED-RDH 60

Query: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120
           G+GAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLL+FGGQ+P+RNSRLEALFNGAPG+
Sbjct: 61  GLGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGI 120

Query: 121 GLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPV 180
           G+TRG+SGDYFIGPGLEELFEQLS NGHR PPPASRSSIDA+PTV+ITQ+HL SDSHCPV
Sbjct: 121 GITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPV 180

Query: 181 CKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240
           CKEKFEL S+ARQM C+HMYHSDCIVPWL+QHNSCPVCRQELPPQG+       XXXXXX
Sbjct: 181 CKEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGI------GXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300
           XXXXXXXXXXXXXXX   VNPGRRNPFS LWPFRSSSS +NHGGAGSSE +NHQMGYSGY
Sbjct: 241 XXXXXXXXXXXXXXXGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGY 299

Query: 301 RYTNSP 307
           RYT+SP
Sbjct: 301 RYTSSP 299

BLAST of Carg26127 vs. NCBI nr
Match: XP_008455755.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455757.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 478.4 bits (1230), Expect = 1.8e-131
Identity = 254/306 (83.01%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MS GRNTHWCYRCR SVR  GRDAVCPNCNGGFI ELDDMV GNPF+FFGLD D++  +H
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDED-RDH 60

Query: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120
           G+GAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLL+FGGQ+P+RNSRLEALFNGAPG+
Sbjct: 61  GLGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGI 120

Query: 121 GLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPV 180
           G+TRG+SGDYFIGPGLEELFE+LS NGHR PPPASRSSIDA+PTV+IT +HL SDSHCPV
Sbjct: 121 GITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPV 180

Query: 181 CKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240
           CKEKFEL S+ARQM C+HMYHSDCIVPWL+QHNSCPVCRQELP QG+      XXXXXXX
Sbjct: 181 CKEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGI------XXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300
           XXXXXXXXXXXXXXXXX VNPGRRNPFS LWPFRSSS     GGAGSSE +NHQMGYSGY
Sbjct: 241 XXXXXXXXXXXXXXXXXXVNPGRRNPFSYLWPFRSSSXXXXXGGAGSSEPSNHQMGYSGY 299

Query: 301 RYTNSP 307
           RYTNSP
Sbjct: 301 RYTNSP 299

BLAST of Carg26127 vs. TAIR10
Match: AT2G40830.2 (RING-H2 finger C1A)

HSP 1 Score: 283.9 bits (725), Expect = 1.2e-76
Identity = 145/237 (61.18%), Postives = 182/237 (76.79%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFF----GLDDDDE 60
           MS+ RNTHWC+RC+ +VR  G++ VC  C GGF+ EL DM   +PF+ F    G+ + D+
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  VHNHGMGAFSAFMRQRLAERN---DMRGRS-ESVFELSPGFGPLLVFGGQVPMR---NSR 120
             +  M AFS FMR RLAER+   ++RGR+  S  E  PG  PLL+FGGQVP R   ++ 
Sbjct: 61  TFDL-MDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNA 120

Query: 121 LEALFN-GAPGVGLTRGNSGDYFIGPGLEELFEQLS-GNGHRRPPPASRSSIDAIPTVRI 180
           +EALFN G+PG+G+TRGN+GDYF GPGLEELFEQLS G   R PPPA RS+IDA+PT++I
Sbjct: 121 VEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKI 180

Query: 181 TQQHLH-SDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELP 224
            Q+HL  SDS+CPVCK++FEL S+A+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP
Sbjct: 181 AQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP 235

BLAST of Carg26127 vs. TAIR10
Match: AT3G56580.2 (RING/U-box superfamily protein)

HSP 1 Score: 273.5 bits (698), Expect = 1.6e-73
Identity = 161/296 (54.39%), Postives = 190/296 (64.19%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELD--------DMVHGNPFEFFGLD 60
           MS+ RNTHWC+RC+ +V  R RDAVC  C GGF+ E+D        D+     F+     
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDL---- 60

Query: 61  DDDEVHNHGMGAFSAFMRQRLAERN---DMRGRSESV-FELSPGFGPLLVFGGQVPMR-- 120
                    M AFSAFMR RLAER+   ++ GR  S   E      PLL+FGGQ P R  
Sbjct: 61  ---------MEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLA 120

Query: 121 ---NSRLEALFNG-APGVGLTRG-NSGDYFIGPGLEELFEQL-SGNGHRRPPPASRSSID 180
              NS +EA  NG APG+G+ RG N+GDYF GPGLEEL EQL SG  HR PPPA +SSID
Sbjct: 121 GGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSID 180

Query: 181 AIPTVRITQQHL-HSDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCR 240
           A+PT++ITQ+HL  SDSHCPVCK++FEL+S+A+QMPC H+YHSDCIVPWLVQHNSCPVCR
Sbjct: 181 ALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCR 240

Query: 241 QELPPQGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRS 276
           +ELP +G                       XXXXXXX   N  RRN FSNLWPFRS
Sbjct: 241 KELPSRG----------------SSSSTQSXXXXXXXGRENSRRRNIFSNLWPFRS 267

BLAST of Carg26127 vs. TAIR10
Match: AT3G10815.1 (RING/U-box superfamily protein)

HSP 1 Score: 142.1 bits (357), Expect = 5.6e-34
Identity = 91/284 (32.04%), Postives = 127/284 (44.72%), Query Frame = 0

Query: 5   RNTHWCYRCRSSVRPRG---RDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNHG 64
           +NTHWC+ CR  +  +G   R+  C  C   F+  L + V  +PF+ FGL   +E  N  
Sbjct: 7   KNTHWCHTCRRGIHLQGEGRREGACIYCGNTFLERLYENVELSPFDLFGL-AYEEARN-- 66

Query: 65  MGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGVG 124
                         RN+ R                 V G Q+       E LFN  P + 
Sbjct: 67  -------------RRNNRRS----------------VIGEQL-----SFEELFNRLPALQ 126

Query: 125 LTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPVC 184
             RG                         PPPAS ++I+++  ++I Q+HL  D +CPVC
Sbjct: 127 DRRG-------------------------PPPASLAAINSLQKIKIRQKHLGLDPYCPVC 186

Query: 185 KEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXXX 244
           +++FE+ S AR+MPC H+YHS+CI+PWLVQ N+CPVCR+ELP                  
Sbjct: 187 QDQFEIGSDARKMPCKHIYHSECILPWLVQRNTCPVCRKELPQDR--------------- 195

Query: 245 XXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGA 286
                             N  R+NP  +LWPFRSS   +NH G+
Sbjct: 247 ------------------NNSRKNPLWHLWPFRSSGLASNHNGS 195

BLAST of Carg26127 vs. TAIR10
Match: AT3G19950.1 (RING/U-box superfamily protein)

HSP 1 Score: 137.1 bits (344), Expect = 1.8e-32
Identity = 90/245 (36.73%), Postives = 121/245 (49.39%), Query Frame = 0

Query: 9   WCYRCRSSVR---PRGRDAVCPNCNGGFIHELDD-----MVHGNP---FEFFGLDD---- 68
           +CY+C  +V        D  CP CN GF+ E +D      ++ NP     FF + D    
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFST 81

Query: 69  -------DDEVHNHGMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQV-PM 128
                         GM   S F           + RS      S  F P       +  +
Sbjct: 82  LLPLIFGSSAAAPSGMDFMSLF-----GPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTL 141

Query: 129 RNS--RLEALFNGAPGVGLTR--GNSGDYFIGPGLEELFEQLSGNGHRR--PPPASRSSI 188
           R+S    E +    P     R  GN GDYF GPGLE+L +QL+ N   R   PPAS+S+I
Sbjct: 142 RSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAI 201

Query: 189 DAIPTVRITQQHLHSD-SHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVC 224
           DA+PTV++T+  L S+ + C VC ++FE  S  +QMPC H++H DC++PWL  HNSCPVC
Sbjct: 202 DALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVC 261

BLAST of Carg26127 vs. TAIR10
Match: AT1G60360.1 (RING/U-box superfamily protein)

HSP 1 Score: 119.0 bits (297), Expect = 5.1e-27
Identity = 84/257 (32.68%), Postives = 113/257 (43.97%), Query Frame = 0

Query: 8   HWCYRCRSSVR---PRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNHGMGA 67
           +WCY C   VR       +  CP C   F+ E++       F F          NH    
Sbjct: 24  YWCYHCDRMVRIASSNPSEIACPRCLRQFVVEIETRQRPR-FTF----------NHATPP 83

Query: 68  FSAFMRQRLAERND----------------MRGRSESVFELSPGFGPL--------LVFG 127
           F A    RL E                   +R RS ++ E      P          V  
Sbjct: 84  FDASPEARLLEALSLMFEPATIGRFGADPFLRARSRNILEPESRPRPQHRRRHSLDNVNN 143

Query: 128 GQVPMRNSRLEALFNGAPGVGL------------TRGNSGDYFIG-PGLEELFEQLSGNG 187
           G +P+       L    P   L               NS DYF G   LE+L EQL+ + 
Sbjct: 144 GGLPLPRRTYVILRPNNPTSPLGNIIAPPNQAPPRHVNSHDYFTGASSLEQLIEQLTQDD 203

Query: 188 HRRPPPASRSSIDAIPTVRITQQHLHSD-SHCPVCKEKFELESKARQMPCSHMYHSDCIV 224
              PPPAS  +I+++P+V+IT QHL +D S C VC E+F +   A ++PC H+YH DCIV
Sbjct: 204 RPGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIV 263

BLAST of Carg26127 vs. Swiss-Prot
Match: sp|O22197|RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.1e-75
Identity = 145/237 (61.18%), Postives = 182/237 (76.79%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFF----GLDDDDE 60
           MS+ RNTHWC+RC+ +VR  G++ VC  C GGF+ EL DM   +PF+ F    G+ + D+
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  VHNHGMGAFSAFMRQRLAERN---DMRGRS-ESVFELSPGFGPLLVFGGQVPMR---NSR 120
             +  M AFS FMR RLAER+   ++RGR+  S  E  PG  PLL+FGGQVP R   ++ 
Sbjct: 61  TFDL-MDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNA 120

Query: 121 LEALFN-GAPGVGLTRGNSGDYFIGPGLEELFEQLS-GNGHRRPPPASRSSIDAIPTVRI 180
           +EALFN G+PG+G+TRGN+GDYF GPGLEELFEQLS G   R PPPA RS+IDA+PT++I
Sbjct: 121 VEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKI 180

Query: 181 TQQHLH-SDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELP 224
            Q+HL  SDS+CPVCK++FEL S+A+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP
Sbjct: 181 AQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP 235

BLAST of Carg26127 vs. Swiss-Prot
Match: sp|Q94AK4|RZF1_ARATH (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.9e-72
Identity = 161/296 (54.39%), Postives = 190/296 (64.19%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELD--------DMVHGNPFEFFGLD 60
           MS+ RNTHWC+RC+ +V  R RDAVC  C GGF+ E+D        D+     F+     
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDL---- 60

Query: 61  DDDEVHNHGMGAFSAFMRQRLAERN---DMRGRSESV-FELSPGFGPLLVFGGQVPMR-- 120
                    M AFSAFMR RLAER+   ++ GR  S   E      PLL+FGGQ P R  
Sbjct: 61  ---------MEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLA 120

Query: 121 ---NSRLEALFNG-APGVGLTRG-NSGDYFIGPGLEELFEQL-SGNGHRRPPPASRSSID 180
              NS +EA  NG APG+G+ RG N+GDYF GPGLEEL EQL SG  HR PPPA +SSID
Sbjct: 121 GGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSID 180

Query: 181 AIPTVRITQQHL-HSDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCR 240
           A+PT++ITQ+HL  SDSHCPVCK++FEL+S+A+QMPC H+YHSDCIVPWLVQHNSCPVCR
Sbjct: 181 ALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCR 240

Query: 241 QELPPQGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRS 276
           +ELP +G                       XXXXXXX   N  RRN FSNLWPFRS
Sbjct: 241 KELPSRG----------------SSSSTQSXXXXXXXGRENSRRRNIFSNLWPFRS 267

BLAST of Carg26127 vs. Swiss-Prot
Match: sp|Q8LPN7|RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.2e-31
Identity = 90/245 (36.73%), Postives = 121/245 (49.39%), Query Frame = 0

Query: 9   WCYRCRSSVR---PRGRDAVCPNCNGGFIHELDD-----MVHGNP---FEFFGLDD---- 68
           +CY+C  +V        D  CP CN GF+ E +D      ++ NP     FF + D    
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFST 81

Query: 69  -------DDEVHNHGMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQV-PM 128
                         GM   S F           + RS      S  F P       +  +
Sbjct: 82  LLPLIFGSSAAAPSGMDFMSLF-----GPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTL 141

Query: 129 RNS--RLEALFNGAPGVGLTR--GNSGDYFIGPGLEELFEQLSGNGHRR--PPPASRSSI 188
           R+S    E +    P     R  GN GDYF GPGLE+L +QL+ N   R   PPAS+S+I
Sbjct: 142 RSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAI 201

Query: 189 DAIPTVRITQQHLHSD-SHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVC 224
           DA+PTV++T+  L S+ + C VC ++FE  S  +QMPC H++H DC++PWL  HNSCPVC
Sbjct: 202 DALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVC 261

BLAST of Carg26127 vs. Swiss-Prot
Match: sp|P0CH30|RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 6.8e-29
Identity = 58/101 (57.43%), Postives = 77/101 (76.24%), Query Frame = 0

Query: 126 NSGDYFIGPGLEELFEQLSGNGHRR--PPPASRSSIDAIPTVRITQQHLHSD-SHCPVCK 185
           N GDYFIGPGLE+L +QL+ N   R   PPAS+S+I+A+P V IT+ +L+S+ + C VC 
Sbjct: 169 NIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCM 228

Query: 186 EKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELP 224
           + FE  ++A+QMPC H+YH DC++PWL  HNSCPVCR ELP
Sbjct: 229 DDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELP 269

BLAST of Carg26127 vs. Swiss-Prot
Match: sp|Q91YL2|RN126_MOUSE (E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 9.1e-26
Identity = 85/270 (31.48%), Postives = 117/270 (43.33%), Query Frame = 0

Query: 8   HWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHG----------------NPFE---- 67
           ++C+ C   + PR  D +CP C  GFI EL +                     PFE    
Sbjct: 11  YFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRNTENGSAPSTAPTDQNRQPFENVDQ 70

Query: 68  ------------FFGL-DDDDEVHNHGMGAFS------AFMRQRLAERNDMRGRSESVFE 127
                        FG+ DD  E+     GA +         R+R  +     G  +    
Sbjct: 71  HLFTLPQGYSQFAFGIFDDSFEIPTFPPGAQADDGRDPESRREREHQSRHRYGARQPRAR 130

Query: 128 LSPGFGPLLVFGGQVPMRNSRLEALFNG------APGVGL-----TRGNSGDYFIGP-GL 187
           L+         G  VP     ++ L NG       P +GL        N  DY  G  GL
Sbjct: 131 LTARRATGRHEG--VPTLEGIIQQLVNGIISPAAVPSLGLGPWGVLHSNPMDYAWGANGL 190

Query: 188 EELFEQLSGN-GHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPVCKEKFELESKARQMP 226
           + +  QL     +  PPPA +  I A+PTV +T++H+ S   CPVCKE + L    RQ+P
Sbjct: 191 DTIITQLLNQFENTGPPPADKEKIQALPTVPVTEEHVGSGLECPVCKEDYALGESVRQLP 250

BLAST of Carg26127 vs. TrEMBL
Match: tr|A0A0A0L0T9|A0A0A0L0T9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.6e-136
Identity = 257/306 (83.99%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MS GRNTHWCYRCR SVR RGRDAVCPNCNGGFI ELDDMV GNPF+FFGLD D++  +H
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDED-RDH 60

Query: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120
           G+GAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLL+FGGQ+P+RNSRLEALFNGAPG+
Sbjct: 61  GLGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGI 120

Query: 121 GLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPV 180
           G+TRG+SGDYFIGPGLEELFEQLS NGHR PPPASRSSIDA+PTV+ITQ+HL SDSHCPV
Sbjct: 121 GITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPV 180

Query: 181 CKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240
           CKEKFEL S+ARQM C+HMYHSDCIVPWL+QHNSCPVCRQELPPQG+       XXXXXX
Sbjct: 181 CKEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGI------GXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300
           XXXXXXXXXXXXXXX   VNPGRRNPFS LWPFRSSSS +NHGGAGSSE +NHQMGYSGY
Sbjct: 241 XXXXXXXXXXXXXXXGSRVNPGRRNPFSYLWPFRSSSSNSNHGGAGSSEPSNHQMGYSGY 299

Query: 301 RYTNSP 307
           RYT+SP
Sbjct: 301 RYTSSP 299

BLAST of Carg26127 vs. TrEMBL
Match: tr|A0A1S3C178|A0A1S3C178_CUCME (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103495857 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 1.2e-131
Identity = 254/306 (83.01%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MS GRNTHWCYRCR SVR  GRDAVCPNCNGGFI ELDDMV GNPF+FFGLD D++  +H
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDED-RDH 60

Query: 61  GMGAFSAFMRQRLAERNDMRGRSESVFELSPGFGPLLVFGGQVPMRNSRLEALFNGAPGV 120
           G+GAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLL+FGGQ+P+RNSRLEALFNGAPG+
Sbjct: 61  GLGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGI 120

Query: 121 GLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHLHSDSHCPV 180
           G+TRG+SGDYFIGPGLEELFE+LS NGHR PPPASRSSIDA+PTV+IT +HL SDSHCPV
Sbjct: 121 GITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPV 180

Query: 181 CKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXXXXXXXXXX 240
           CKEKFEL S+ARQM C+HMYHSDCIVPWL+QHNSCPVCRQELP QG+      XXXXXXX
Sbjct: 181 CKEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGI------XXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGAGSSEATNHQMGYSGY 300
           XXXXXXXXXXXXXXXXX VNPGRRNPFS LWPFRSSS     GGAGSSE +NHQMGYSGY
Sbjct: 241 XXXXXXXXXXXXXXXXXXVNPGRRNPFSYLWPFRSSSXXXXXGGAGSSEPSNHQMGYSGY 299

Query: 301 RYTNSP 307
           RYTNSP
Sbjct: 301 RYTNSP 299

BLAST of Carg26127 vs. TrEMBL
Match: tr|A0A2N9I8M8|A0A2N9I8M8_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS38476 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 6.6e-106
Identity = 213/315 (67.62%), Postives = 246/315 (78.10%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGL--DDDDEVH 60
           MS+GRNTHWCY C   VR RGRDA CP+CNGGF+ ELDDM+  +PF+ FGL  D+D +  
Sbjct: 1   MSSGRNTHWCYNCSRPVRLRGRDAACPSCNGGFVQELDDMMRASPFDLFGLDIDEDRDRR 60

Query: 61  NHGMGAFSAFMRQRLAER---NDMRGRSESVFELSPGFGPLLVFGGQVPMR---NSRLEA 120
           +  M AFSAFM QRLA+R   +D+R RS++V E +PGFGPLL+FGGQ+P+R   N   EA
Sbjct: 61  SRVMEAFSAFMGQRLADRSRSHDIRARSDAVPEHTPGFGPLLIFGGQIPIRLSGNGGFEA 120

Query: 121 LFNGAPGVGLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHL 180
           LFNG PG+GLTRGN+GDYFIGPGLEELFEQLS N  R PPPA+RSSIDA+PT++ITQ+HL
Sbjct: 121 LFNGTPGIGLTRGNAGDYFIGPGLEELFEQLSANDRRGPPPAARSSIDAMPTIKITQRHL 180

Query: 181 HSDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXX 240
            SDSHCPVCKEKFEL S+ARQM C+HMYHSDCIVPWLVQHNSCPVCRQELPPQG+     
Sbjct: 181 RSDSHCPVCKEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQGLSNGRS 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNH-GGAGSSEAT 300
            XXXXXXXXXXXXXXX           + GRRNPFSN WPFRSS+S +NH G AGSS  T
Sbjct: 241 SXXXXXXXXXXXXXXXRENSRE-----SQGRRNPFSNFWPFRSSTSSSNHNGNAGSSSQT 300

Query: 301 ----NHQMGYSGYRY 303
               N QMGYSG+ +
Sbjct: 301 IHEQNSQMGYSGWPF 310

BLAST of Carg26127 vs. TrEMBL
Match: tr|A0A2I4GID8|A0A2I4GID8_9ROSI (probable E3 ubiquitin-protein ligase RHC1A OS=Juglans regia OX=51240 GN=LOC109008136 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 7.6e-102
Identity = 214/316 (67.72%), Postives = 240/316 (75.95%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MSNGRNTHWCYRC   VR RGRDAVCP CNGGFI ELDDMVH +PF+FFGLD D+     
Sbjct: 1   MSNGRNTHWCYRCNRPVRLRGRDAVCPGCNGGFIQELDDMVHISPFDFFGLDVDENHDRR 60

Query: 61  G--MGAFSAFMRQRLAER---NDMRGRSESVFELSPGFGPLLVFGGQVPMR---NSRLEA 120
              M AFSAFMR RLA+R   +D+R RS+SV E +PGFGPLL+FGGQ+P R   N  +EA
Sbjct: 61  SGFMEAFSAFMRHRLADRSHSHDIRARSDSVPEHAPGFGPLLIFGGQIPFRLSGNGGIEA 120

Query: 121 LFNGAPGVGLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHL 180
           + NG PG+GLTRGN+GDYFIGPGLEELFEQ S N  R PPPASRSSIDA+PT++I Q+HL
Sbjct: 121 ILNGTPGIGLTRGNAGDYFIGPGLEELFEQHSANDRRGPPPASRSSIDAMPTIKIAQRHL 180

Query: 181 HSDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXX 240
            SDSHCPVCK+KFEL S+ARQMPC HMYHSDCI+PWLVQHNSCPVCRQELPPQG+     
Sbjct: 181 RSDSHCPVCKDKFELGSEARQMPCYHMYHSDCIIPWLVQHNSCPVCRQELPPQGI----N 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNH--GGAGSSEA 300
                 XXXXXXXXXXXXXXXXXXX         FS LWPFRSS S +NH  G AGSS  
Sbjct: 241 IGRSSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXFSYLWPFRSSHSSSNHNNGTAGSSSP 300

Query: 301 TNHQ----MGYSGYRY 303
           T H+     GYSG+ +
Sbjct: 301 TIHENNQNTGYSGWPF 312

BLAST of Carg26127 vs. TrEMBL
Match: tr|B9HDT7|B9HDT7_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G032100v3 PE=4 SV=2)

HSP 1 Score: 377.9 bits (969), Expect = 2.2e-101
Identity = 192/315 (60.95%), Postives = 225/315 (71.43%), Query Frame = 0

Query: 1   MSNGRNTHWCYRCRSSVRPRGRDAVCPNCNGGFIHELDDMVHGNPFEFFGLDDDDEVHNH 60
           MS+ RNTHWCY CR  VR RGRDA CP C+GGF+ ELDDM   NP +FFG+D DD+  N 
Sbjct: 1   MSSSRNTHWCYSCRRPVRLRGRDAACPYCSGGFVQELDDMHRINPLDFFGMDGDDDHDNM 60

Query: 61  G--MGAFSAFMRQRLAER---NDMRGRSESVFELSPGFGPLLVFGGQVPMR---NSRLEA 120
              M AFSAFMRQR+A+R   +D+R RS+S+ E +PGFGPLL+FGGQ+P R   N  LEA
Sbjct: 61  FGLMEAFSAFMRQRMADRSHNHDIRSRSDSIPEHNPGFGPLLIFGGQIPFRLSGNGGLEA 120

Query: 121 LFNGAPGVGLTRGNSGDYFIGPGLEELFEQLSGNGHRRPPPASRSSIDAIPTVRITQQHL 180
           LF+G+PGV   RGN+GDYF+GPGLEELFEQLS N  R P PA+RSSIDA+PTV+ITQ+HL
Sbjct: 121 LFSGSPGVAFARGNAGDYFVGPGLEELFEQLSANDRRGPAPATRSSIDAMPTVKITQRHL 180

Query: 181 HSDSHCPVCKEKFELESKARQMPCSHMYHSDCIVPWLVQHNSCPVCRQELPPQGVXXXXX 240
            +DSHCPVCK+KFEL S+ARQMPC+H+YHSDCIVPWLVQHNSCPVCRQELPPQG      
Sbjct: 181 RTDSHCPVCKDKFELGSEARQMPCNHLYHSDCIVPWLVQHNSCPVCRQELPPQG------ 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXSVNPGRRNPFSNLWPFRSSSSRTNHGGA-GSSEAT 300
                                    +   GRRNP S LWPFRSS+S +NH    GSS  T
Sbjct: 241 --SNSGHSYQSSSSRSRNSNYSGRENSREGRRNPLSYLWPFRSSNSSSNHDETPGSSSPT 300

Query: 301 ----NHQMGYSGYRY 303
               NHQMGYSG+ +
Sbjct: 301 MHENNHQMGYSGWPF 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022950677.12.5e-15797.06probable E3 ubiquitin-protein ligase RHC1A [Cucurbita moschata] >XP_022950678.1 ... [more]
XP_023543475.11.8e-15594.77probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022967862.14.2e-15289.87probable E3 ubiquitin-protein ligase RHC1A [Cucurbita maxima] >XP_022967863.1 pr... [more]
XP_004144313.12.4e-13683.99PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus] >XP_00414431... [more]
XP_008455755.11.8e-13183.01PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1... [more]
Match NameE-valueIdentityDescription
AT2G40830.21.2e-7661.18RING-H2 finger C1A[more]
AT3G56580.21.6e-7354.39RING/U-box superfamily protein[more]
AT3G10815.15.6e-3432.04RING/U-box superfamily protein[more]
AT3G19950.11.8e-3236.73RING/U-box superfamily protein[more]
AT1G60360.15.1e-2732.68RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
sp|O22197|RHC1A_ARATH2.1e-7561.18Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
sp|Q94AK4|RZF1_ARATH2.9e-7254.39E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
sp|Q8LPN7|RNG1L_ARATH3.2e-3136.73E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
sp|P0CH30|RING1_GOSHI6.8e-2957.43E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
sp|Q91YL2|RN126_MOUSE9.1e-2631.48E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0L0T9|A0A0A0L0T9_CUCSA1.6e-13683.99Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE=4 SV=1[more]
tr|A0A1S3C178|A0A1S3C178_CUCME1.2e-13183.01E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103495857 P... [more]
tr|A0A2N9I8M8|A0A2N9I8M8_FAGSY6.6e-10667.62Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS38476 PE=4 SV=1[more]
tr|A0A2I4GID8|A0A2I4GID8_9ROSI7.6e-10267.72probable E3 ubiquitin-protein ligase RHC1A OS=Juglans regia OX=51240 GN=LOC10900... [more]
tr|B9HDT7|B9HDT7_POPTR2.2e-10160.95Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G032100v3 PE=... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26127-RACarg26127-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 178..218
e-value: 2.0E-7
score: 40.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 177..219
e-value: 3.6E-11
score: 43.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 178..219
score: 12.43
NoneNo IPR availablePFAMPF14369zinc_ribbon_9coord: 6..37
e-value: 1.2E-11
score: 44.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..306
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 229..306
NoneNo IPR availablePANTHERPTHR44290FAMILY NOT NAMEDcoord: 1..301
NoneNo IPR availablePANTHERPTHR44290:SF1E3 UBIQUITIN LIGASE-RELATEDcoord: 1..301
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 178..219
e-value: 1.29503E-23
score: 90.4548
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 158..225
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 142..225
e-value: 7.9E-21
score: 75.5