Carg25977 (gene) Silver-seed gourd

NameCarg25977
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionE3 ubiquitin-protein ligase RHF2A
LocationCucurbita_argyrosperma_scaffold_311 : 51009 .. 56584 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATACGCACTGACCCGACGACCGACCCAAGCGCAGCACTCAGCAGCAGCCTTTTATCAAGTCAAGGACGGAGCCAATCCTCCTCCTCCTCCTCCGCCTGCTCCTCCTCCTAGGGTTCCACTGGTTCCAGTCCAATCTTATTTACCTGTTCTCTCACAAGCTTCTCCTGCTGATCGTCGTTTCTCATGGAGGTAACTTTCTTCCCCTAAATCTCTTCTCTCTTCCTTCTTTTTGCGATTTCATGTCTCCTATCTTTAATTCTCTCTTTGATGAATATTATTGTGCCTACTTTTCTTCCTTTTTTCCGGATTCCGTTTTTCTTGGCTGTTGTTCACCGGATGGATTTATTCTGTTACTGGATTCTTTTACTTTTTTTTAATCTTGTTTTCATTGAGTATCGGAACTGGAGAGGAATGGCTTTGTTATCCTTTTCAAATGTATTTTCCTCTTGAATTTTCATTCTGGCTATCTCTATTTGTAGTTTTTGCGATCTTTTATTACTGTTCTCTTCGGGCTAGCTGCTTGTTGCTTTTCGCTCTGGAAATTGTGGTTTTGAATTCTTTGATTCTTTATGGATGTTAGGGTAGGCTGTTTGTGCGTGGTTTAATTCACTGAAAATTTGTGTCGTGTCGCTAATTTATAATTGTTAGCACTTGTTTATCATTTCCTTGTTTAGTTGCTGCTTATATATCACGCAATAGATCATGTGTTTCTTGTATATTTTTAAGTTTTTCTTGGTGATGATGATTATTTTTTTTTTTAAAATTCGTGTAGTCTCCAACGATGGACGAAACTAAGAATTCTGAAGCGCATTTGACATCAGCTGCAGCTTTTGTCGAAGGTGGAATCCAGGATGCTTGTGATGACGCTTGCAGTATATGTCTTGAAAACTTCTGTGACAGTGATCCTTCAACGGTAGTACTTATAGTGTGATTTGATTTTTAATCGGAAGTCAGATGGTTTCTGTTCTACTTAGTCAGTTGCTATATTTTGTTCAGATGACCAGTTGCAAACATGAGTTTCATCTGCAGTGCGTTCTTGAATGGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGCAATATCTGTTTGAGATATCAGATCTGCTTGTGATCATCTTGTCTTACTATTGATTTGATTACGACGACAGCGGATGTTTGGCAGACTACCATATACCTGCCTCTAACTGTGGCTTCTTTTTATATTTATACAGTTTCGTCAATTAAGTTCATTTGATGCACCAATTTGTAACCACCGGGGGTTCTTGGGCATTTCCCTTCTTTTATAATTTTCACCAGCCAATGAAAAGTATCTTATTTCCTTCTTCCTCCAAAAAGAAAAATAAAATTATAGGGAAGTTCTAATACTTCGAAAGGGTTAGTAGATCATCATATAAGTAGAATCTTACGTATTTGAATTGTGAGACGGTTGATTTCTCAGTGCTAGAAATGAATCCAAATTTGAAAGACCATGCTAGGTACTTCGGGGGTGTAAGACTTGATTAAGTATCTGGGTCTGTTGCTATGACAAGTCTTCTATTGACTAGTGTCCAGACTACCAATCTATGATGTACTGGTGGGGGTGGAAGCTGGCAAGAAATATGACTTACTACCCTCATTATTAGTATCACATGTATTTAACATCTAGGTGATAGCTTATAGTGGTAAAGGTGGTGGGAATTTGGATGCTCTTTTTATTTCGTGGTATGATGTGCTGAGTTTCTAATATCATCTTCAGAATGTTGAGACAAGAGAGAAAAAGGTCAGCAGACCTCTTGCCATGTGGGCATTTTCCTTTGATGGTTGAAGAAAATGGTGAGGATGAAATCTAACTTCAAGAGTTGGATTTTGTTTAAAGATTCTTGGATTTAACCTAGTGATCGTGGTTTTAACCCATGCCCAGCATTGCCTGCTTGGAATATTAAAAATTTGACACGGCAAACATTGGGTGGCTTGAAGATTTTGGGTAGAAAATTGATGTAATTCATGTGAGGATTAAACCATACATGAATACTTATTTTTATTGCTTTTTCATTCTCTTACTCAGTTGAAAAAAGTTGAACTTTATAAAATATAATTCCAAATTGCAAAAAATATAATTCTCAAAACCAGCTAACGGATATGGACTTCAAGGCAAGACAGTCTTCCTGATGATCGTTCCTGTAAAATACCAACCTCTTATGCATTAAAGCTTAACTTGACATTCCATCTTATCTTCTGAAACCAACACTGCTTTTGATGTGATCATACTTGGAAATTAAGTGCACAATATCTTTGCAGACCCGTTTAGCAAATATTTATCTCCCTTTCAATGTTAAGTTGTGAGTTTTTCAAATATTTTTGCAGATGCCTAATTTTTTTTGAATGTTCATCTATTTCATTGATGTGCGTTTGCATCTTTTCTGCATAATAGTTTGCTGCGGATCACTTTTTACAAGTTTGGATTGCAATTGATCTCTAATATATGTGATTTCAACATATTGAATCCCGACGAAGAAAAGAAAGAGAGTTTTTGTGGATAGTATCCATTGCAAGATCGTTTCCTAGACTTGAATATGAGCCATAGTTGTTTGCTACCCTATTCTGTAAGCTCTATCATTGGAAAAAGCTAAGGATCACTGGATTCTCATAACCATTTTTTTGACGTTTCTTTTTGGAAAAAAATTCTCTGATTTTAACCATGGGTCAATGACTCAATCTTATGGAACTGTGACAAGGAAGACCTACTTTGGTGTCCTTGGCCTAAAATAGAGAAGACCTACTTTGGAAGTGTGATAATCTGGGTCTTTTATAACCAAATATCTATTAACTGATTTACGGGAGCCTTCTGTTTTGGATGGTACTTTATGCTTCTTCCTCTAACGCTTTTTTCTTAAAAAAAGGTTGGTGCTTTCTTTGGCAGTTTTCTCATGAAGGAATTATTACTTAGTACAAGATCCAGAAGAGGCACCCCTGTATTGTTCTTTTTTTAAAATCTGGTCTTGGTCATATATTGAAGTGAAACAAAATAGATTGGTTTGGGCTTGATTGAGTGAACAAGAACTCAGAACACCCAAGGACTTTCACCACACTTTTTCTTTTTTTCTTTTTTAATTTTTACAAGAACTACTGATTGTTCATATCATTTTGAATTGTTCATTCACTTTCTCCTGTGTGTCCCAACTTTCTGACAGTCAACATGGAATCTAACCTTCATCTTTTCAGTGAGCTTTGTCTTGCTTCTATTTACATAAAAGTACTATAAACCAATCATCATTAAACAAAAAATCATTTAAACATATTTCCATTCAGAATACCGAGCTGTCTAATTACTTAGATTATTAACTAACAATAAGTTGGCTTGGATAGTCCATGATCTGCTTCGTAGGCTGCTTTTGTTGTGATTCTGATCCTATGAATCATATATTTTGTTATTGCTAGATTGAGGGGTTTGTGTTGTTTATATATTTCTTTCATTTTTTGCCATGATGCTTTATGGTGGTAGGGCTCAGTTGGGAGTGGTTTTCTTTCAGTTCTATTGTATGATAGTTTTTTTAGGGAAGGTTCAAATTTCAACTTTCTTAGACTAGAATTCAGGCGGTTATTGTTTTAAGTAGAAATGTAAAACATATTATTATTTTTTTTAAGAAGGTTGGAAATTATATAAAGCTTCACAGTGAATAAAAAATGGGAAGAACATTTTTTGAATTGAAAAGCCAGACCAGAAACGTGACATTAATCTGGAAAGAAATTGGAATCCCGTCTTCATCAAGCAAATTATTCTGAAATTTCTCAAGTACCTCTCTTTAGCCAGATACTCCACAACATGATCAATACTCCCAGCTCTGCCGAAGTTGTGCATTATTTTCTGCATTAATATGAAGTTTGAGGTTATGACTGGAATTTAAATTTGATTGGTTCAAGCATTTTTATTTTGTCCCATCTTTTAATAGGTGTCAGAGAAGCTCCCAATGCCCCATGTGTTGGCAACCTGTTAGTCTGAAGGATCCAACCAGGTTTATACTGATTCCATTTTTGTATCATCACTTTTTCATTTAAATTTGAAAGCAGATGAAGGTTGTTTATGTGAGGTTGTTCTGCAGTCAGGAGTTGCTCGAAGCAGTAGAACAAGAGAGAAGTTTTAGACTTAATCCAGTTAGAAACTCAACAATATTTCGCCATCCCACTTTTGGTGACTTTGAGTTACAGCATGTATGTATCCAATATGATATTTACCCTAAAGTCACTAAATCATGGACACCAAAATCTAAGTTGGGGAACGTTTTTTCTCAATGCAGCTGCCAGCTGGTGCTGATGATGCTGACCTTGAAGAGCGTATACTCCAACACCTAGCTGCTGCAGCTGCCATGGGACAAGCTCGCCATAGTGCTAGAAGGGAAGGCCACAGACATCGGTCAGGAATTCATGGCCGTCCCCAATTGTTGGTGTTTTCTTCCCCTCCTAATTCTTCATCGACCTCTCCACTTCCTCGTGACAATCGGGAAGGTGAAGCAGCCTCAAGAATCATGGTAGCTACTGTATCACCACCTCAACCTGCTGGAGAAGAGTCTCCACAACTCAATGCTTCAGCATCTCCCGTGCAAACTGATCAGTCTTCTCCATCAGCACCTCAATACAGTTCACGTTCTGCCAGCCAACTTGGATCTCCTTCGAGCGATAGGTATTCATTATTCATTGGATTTAATTGAAATGTGTCCATCGCATGTAGCTACATGGAATTTATCGGTCATGTATTAATATTATTGTCCCACACCGTTCTTAGGAGATCCACCAGTCAACCAGTGCCAAGTAGTCAAGATAGAGCCGGGCCATCATCAGAATTGCAATCACTCTCGGATTCACTGAAGTCTCGATTTAATGCAATGTCAATGAGGTTTGTAATATGTCTTAATGGCTTTTATTGTTTTTAGTGTAATTTTTGATTACTTTGTTCTTTCATGACCTGAGCACCTCCATTATCCTTTTCTTTTCAGATACAAAGATTCAATCGCAAAGAGCACACGAGGCTGGAAGGAGAAATTGTTCTCTCGCAATACTTCAATGTCAGATATTGGCTCTGAGGTTAGAAGAGAGGTTAATGTTGGAATAGCAACGGTATCGCGCATGATGGAACGTCTTGAAACAAGAGATGCTAGAAAAAGTAGCAGTCCTGAGTCCAGTAATGTAGGGGATAACAACCCAGTTTCGGAATCAAATGATAGACGGACACCAAAGAATGGAGGAAACAATCCTTTGAGCGTCTCAAATAAAGAAGGTTCATGCCCTGCAGAATCCAGTTCAAGTTGAACATTCTCAGAATTGGCTTCACTCAAAGGCCATCTTATAAGAGGTGAATACTGCAATTGATTGTTTCCTTCTTCCATACTACAAGAAATAGCCTGTAGTAGACTGAATGTTTTAAAAAAAATGGAAAAAAAAAAAAAGAAAAAAGAAAGAAAGGGAACTTCGAAATATTCACAAAAATGACTGGGCTTTAGTTTATTTTATAATTTGTTAAGAGTCAATTTAAATATTAGTCCATGTAGTAAATAGCTTATTTACTCGTAAATTGACACTGTCATAGTTTG

mRNA sequence

TCATACGCACTGACCCGACGACCGACCCAAGCGCAGCACTCAGCAGCAGCCTTTTATCAAGTCAAGGACGGAGCCAATCCTCCTCCTCCTCCTCCGCCTGCTCCTCCTCCTAGGGTTCCACTGGTTCCAGTCCAATCTTATTTACCTGTTCTCTCACAAGCTTCTCCTGCTGATCGTCGTTTCTCATGGAGTCTCCAACGATGGACGAAACTAAGAATTCTGAAGCGCATTTGACATCAGCTGCAGCTTTTGTCGAAGGTGGAATCCAGGATGCTTGTGATGACGCTTGCAGTATATGTCTTGAAAACTTCTGTGACAGTGATCCTTCAACGATGACCAGTTGCAAACATGAGTTTCATCTGCAGTGCGTTCTTGAATGGTGTCAGAGAAGCTCCCAATGCCCCATGTGTTGGCAACCTGTTAGTCTGAAGGATCCAACCAGTCAGGAGTTGCTCGAAGCAGTAGAACAAGAGAGAAGTTTTAGACTTAATCCAGTTAGAAACTCAACAATATTTCGCCATCCCACTTTTGGTGACTTTGAGTTACAGCATCTGCCAGCTGGTGCTGATGATGCTGACCTTGAAGAGCGTATACTCCAACACCTAGCTGCTGCAGCTGCCATGGGACAAGCTCGCCATAGTGCTAGAAGGGAAGGCCACAGACATCGGTCAGGAATTCATGGCCGTCCCCAATTGTTGGTGTTTTCTTCCCCTCCTAATTCTTCATCGACCTCTCCACTTCCTCGTGACAATCGGGAAGGTGAAGCAGCCTCAAGAATCATGGTAGCTACTGTATCACCACCTCAACCTGCTGGAGAAGAGTCTCCACAACTCAATGCTTCAGCATCTCCCGTGCAAACTGATCAGTCTTCTCCATCAGCACCTCAATACAGTTCACGTTCTGCCAGCCAACTTGGATCTCCTTCGAGCGATAGGAGATCCACCAGTCAACCAGTGCCAAGTAGTCAAGATAGAGCCGGGCCATCATCAGAATTGCAATCACTCTCGGATTCACTGAAGTCTCGATTTAATGCAATGTCAATGAGATACAAAGATTCAATCGCAAAGAGCACACGAGGCTGGAAGGAGAAATTGTTCTCTCGCAATACTTCAATGTCAGATATTGGCTCTGAGGTTAGAAGAGAGGTTAATGTTGGAATAGCAACGGTATCGCGCATGATGGAACGTCTTGAAACAAGAGATGCTAGAAAAAGTAGCAGTCCTGAGTCCAGTAATGTAGGGGATAACAACCCAGTTTCGGAATCAAATGATAGACGGACACCAAAGAATGGAGGAAACAATCCTTTGAGCGTCTCAAATAAAGAAGGTTCATGCCCTGCAGAATCCAGTTCAAGTTGAACATTCTCAGAATTGGCTTCACTCAAAGGCCATCTTATAAGAGGTGAATACTGCAATTGATTGTTTCCTTCTTCCATACTACAAGAAATAGCCTGTAGTAGACTGAATGTTTTAAAAAAAATGGAAAAAAAAAAAAAGAAAAAAGAAAGAAAGGGAACTTCGAAATATTCACAAAAATGACTGGGCTTTAGTTTATTTTATAATTTGTTAAGAGTCAATTTAAATATTAGTCCATGTAGTAAATAGCTTATTTACTCGTAAATTGACACTGTCATAGTTTG

Coding sequence (CDS)

ATGGAGTCTCCAACGATGGACGAAACTAAGAATTCTGAAGCGCATTTGACATCAGCTGCAGCTTTTGTCGAAGGTGGAATCCAGGATGCTTGTGATGACGCTTGCAGTATATGTCTTGAAAACTTCTGTGACAGTGATCCTTCAACGATGACCAGTTGCAAACATGAGTTTCATCTGCAGTGCGTTCTTGAATGGTGTCAGAGAAGCTCCCAATGCCCCATGTGTTGGCAACCTGTTAGTCTGAAGGATCCAACCAGTCAGGAGTTGCTCGAAGCAGTAGAACAAGAGAGAAGTTTTAGACTTAATCCAGTTAGAAACTCAACAATATTTCGCCATCCCACTTTTGGTGACTTTGAGTTACAGCATCTGCCAGCTGGTGCTGATGATGCTGACCTTGAAGAGCGTATACTCCAACACCTAGCTGCTGCAGCTGCCATGGGACAAGCTCGCCATAGTGCTAGAAGGGAAGGCCACAGACATCGGTCAGGAATTCATGGCCGTCCCCAATTGTTGGTGTTTTCTTCCCCTCCTAATTCTTCATCGACCTCTCCACTTCCTCGTGACAATCGGGAAGGTGAAGCAGCCTCAAGAATCATGGTAGCTACTGTATCACCACCTCAACCTGCTGGAGAAGAGTCTCCACAACTCAATGCTTCAGCATCTCCCGTGCAAACTGATCAGTCTTCTCCATCAGCACCTCAATACAGTTCACGTTCTGCCAGCCAACTTGGATCTCCTTCGAGCGATAGGAGATCCACCAGTCAACCAGTGCCAAGTAGTCAAGATAGAGCCGGGCCATCATCAGAATTGCAATCACTCTCGGATTCACTGAAGTCTCGATTTAATGCAATGTCAATGAGATACAAAGATTCAATCGCAAAGAGCACACGAGGCTGGAAGGAGAAATTGTTCTCTCGCAATACTTCAATGTCAGATATTGGCTCTGAGGTTAGAAGAGAGGTTAATGTTGGAATAGCAACGGTATCGCGCATGATGGAACGTCTTGAAACAAGAGATGCTAGAAAAAGTAGCAGTCCTGAGTCCAGTAATGTAGGGGATAACAACCCAGTTTCGGAATCAAATGATAGACGGACACCAAAGAATGGAGGAAACAATCCTTTGAGCGTCTCAAATAAAGAAGGTTCATGCCCTGCAGAATCCAGTTCAAGTTGA

Protein sequence

MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSSTSPLPRDNREGEAASRIMVATVSPPQPAGEESPQLNASASPVQTDQSSPSAPQYSSRSASQLGSPSSDRRSTSQPVPSSQDRAGPSSELQSLSDSLKSRFNAMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSESNDRRTPKNGGNNPLSVSNKEGSCPAESSSS
BLAST of Carg25977 vs. NCBI nr
Match: XP_022925357.1 (E3 ubiquitin-protein ligase RHF2A-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 596.7 bits (1537), Expect = 5.8e-167
Identity = 387/390 (99.23%), Postives = 389/390 (99.74%), Query Frame = 0

Query: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60
           + SPTMDETKNSEAHLTSAAAFV+GGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ
Sbjct: 3   VRSPTMDETKNSEAHLTSAAAFVKGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 62

Query: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120
           CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL
Sbjct: 63  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 122

Query: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180
           QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS
Sbjct: 123 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 182

Query: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 183 STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWK 300
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWK
Sbjct: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWK 302

Query: 301 EKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSES 360
           EKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSES
Sbjct: 303 EKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSES 362

Query: 361 NDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           NDRRTXXXXXXXXXXXXXKEGSCPAESSSS
Sbjct: 363 NDRRTXXXXXXXXXXXXXKEGSCPAESSSS 392

BLAST of Carg25977 vs. NCBI nr
Match: XP_022925353.1 (E3 ubiquitin-protein ligase RHF2A-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 589.0 bits (1517), Expect = 1.2e-164
Identity = 350/408 (85.78%), Postives = 351/408 (86.03%), Query Frame = 0

Query: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60
           MESPTMDETKNSEAHLTSAAAFV+GGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ
Sbjct: 1   MESPTMDETKNSEAHLTSAAAFVKGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60

Query: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120
           CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL
Sbjct: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120

Query: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180
           QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS
Sbjct: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180

Query: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEE------------------XXXXXXXXXX 240
           STSPLPRDNREGEAASRIMVATVSPPQPAGEE                  XXXXXXXXXX
Sbjct: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFN 300
           XXXXXXXX                                       LQSLSDSLKSRFN
Sbjct: 241 XXXXXXXXDSYMEFIGHVLILLSHTVLRRSTSQPVPSSQDRAGPSSELQSLSDSLKSRFN 300

Query: 301 AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARK 360
           AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARK
Sbjct: 301 AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARK 360

Query: 361 SSSPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           SSSPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS
Sbjct: 361 SSSPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS 408

BLAST of Carg25977 vs. NCBI nr
Match: XP_022925348.1 (E3 ubiquitin-protein ligase RHF2A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 348/408 (85.29%), Postives = 350/408 (85.78%), Query Frame = 0

Query: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60
           + SPTMDETKNSEAHLTSAAAFV+GGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ
Sbjct: 3   VRSPTMDETKNSEAHLTSAAAFVKGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 62

Query: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120
           CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL
Sbjct: 63  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 122

Query: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180
           QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS
Sbjct: 123 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 182

Query: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEE------------------XXXXXXXXXX 240
           STSPLPRDNREGEAASRIMVATVSPPQPAGEE                  XXXXXXXXXX
Sbjct: 183 STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFN 300
           XXXXXXXX                                       LQSLSDSLKSRFN
Sbjct: 243 XXXXXXXXDSYMEFIGHVLILLSHTVLRRSTSQPVPSSQDRAGPSSELQSLSDSLKSRFN 302

Query: 301 AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARK 360
           AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARK
Sbjct: 303 AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARK 362

Query: 361 SSSPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           SSSPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS
Sbjct: 363 SSSPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS 410

BLAST of Carg25977 vs. NCBI nr
Match: XP_022974093.1 (E3 ubiquitin-protein ligase RHF2A-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 333/408 (81.62%), Postives = 335/408 (82.11%), Query Frame = 0

Query: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60
           MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ
Sbjct: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60

Query: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120
           CVLEWCQRSSQCPMCWQP+SLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL
Sbjct: 61  CVLEWCQRSSQCPMCWQPISLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120

Query: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180
           QHLPAGA DADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS
Sbjct: 121 QHLPAGAGDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180

Query: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEE------------------XXXXXXXXXX 240
           STSPLPRDN+EGEAASRIMVATVSPPQP GEE                  XXXXXXXXXX
Sbjct: 181 STSPLPRDNQEGEAASRIMVATVSPPQPVGEESXXXXXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFN 300
           XXXXXXXX                                       LQSLSDSLKSRFN
Sbjct: 241 XXXXXXXXDSYMEFIGHVLILLSHTILRRSTSQPVSSSQDRAGPSSELQSLSDSLKSRFN 300

Query: 301 AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARK 360
           AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMME LETRDARK
Sbjct: 301 AMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMEHLETRDARK 360

Query: 361 SSSPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           SSSPESSNVGDNNPVSESNDRRT             KEGSCPAESSSS
Sbjct: 361 SSSPESSNVGDNNPVSESNDRRTPKNEGNNPLSVSNKEGSCPAESSSS 408

BLAST of Carg25977 vs. NCBI nr
Match: XP_022974084.1 (E3 ubiquitin-protein ligase RHF2A-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 578.6 bits (1490), Expect = 1.6e-161
Identity = 331/406 (81.53%), Postives = 333/406 (82.02%), Query Frame = 0

Query: 3   SPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCV 62
           SPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCV
Sbjct: 5   SPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCV 64

Query: 63  LEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQH 122
           LEWCQRSSQCPMCWQP+SLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQH
Sbjct: 65  LEWCQRSSQCPMCWQPISLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQH 124

Query: 123 LPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSST 182
           LPAGA DADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSST
Sbjct: 125 LPAGAGDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSST 184

Query: 183 SPLPRDNREGEAASRIMVATVSPPQPAGEE------------------XXXXXXXXXXXX 242
           SPLPRDN+EGEAASRIMVATVSPPQP GEE                  XXXXXXXXXXXX
Sbjct: 185 SPLPRDNQEGEAASRIMVATVSPPQPVGEESXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 244

Query: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAM 302
           XXXXXX                                       LQSLSDSLKSRFNAM
Sbjct: 245 XXXXXXDSYMEFIGHVLILLSHTILRRSTSQPVSSSQDRAGPSSELQSLSDSLKSRFNAM 304

Query: 303 SMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSS 362
           SMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMME LETRDARKSS
Sbjct: 305 SMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMMEHLETRDARKSS 364

Query: 363 SPESSNVGDNNPVSESNDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           SPESSNVGDNNPVSESNDRRT             KEGSCPAESSSS
Sbjct: 365 SPESSNVGDNNPVSESNDRRTPKNEGNNPLSVSNKEGSCPAESSSS 410

BLAST of Carg25977 vs. TAIR10
Match: AT5G22000.2 (RING-H2 group F2A)

HSP 1 Score: 372.1 bits (954), Expect = 4.2e-103
Identity = 204/382 (53.40%), Postives = 241/382 (63.09%), Query Frame = 0

Query: 9   TKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQR 68
           T  SE HLTSAAAFVEGGIQDACDDACSICLE+FC+SDPST+TSCKHE+HLQC+LEWCQR
Sbjct: 7   TTTSEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQR 66

Query: 69  SSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQHLPAGAD 128
           SSQCPMCWQ +SLKDPTSQELLEAVEQER+FR NP RN+TIFRHPT GDFELQHLP G D
Sbjct: 67  SSQCPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVD 126

Query: 129 DADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSSTSPLPRD 188
           +A++EERI+QHLAAAAAMG+ARH  RREGHR RS   G  Q +VFSS PN+SS       
Sbjct: 127 NAEIEERIIQHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPXXXXX 186

Query: 189 NREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 248
                        +  P    GE                                     
Sbjct: 187 XXXXXXXXXSDTVSNLPHNALGE-------------GSHQSNTQPPTSSHPRQVSPSASD 246

Query: 249 XXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWKEKLFSRNT 308
                                LQS S+SLKSR NA+S RYK+SI+K+TR WK++LFSRNT
Sbjct: 247 SNSRPLNQSSPSEQDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNT 306

Query: 309 SMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSESNDRRTXXX 368
           SM+D+GSEV+REV+ GIATVSRMMERLETR+  + S+   S+V +N+    +N+      
Sbjct: 307 SMADLGSEVKREVSAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAA 366

Query: 369 XXXXXXXXXXKEGSCPAESSSS 391
                      + +C   S SS
Sbjct: 367 GDEHSVNERGVKETCATGSGSS 375

BLAST of Carg25977 vs. TAIR10
Match: AT4G14220.1 (RING-H2 group F1A)

HSP 1 Score: 131.0 bits (328), Expect = 1.6e-30
Identity = 119/313 (38.02%), Postives = 161/313 (51.44%), Query Frame = 0

Query: 35  CSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVE 94
           CSICLE F   DPST+TSCKHE+HLQC++EW QRS +CP+CWQ   L+DP SQELL AVE
Sbjct: 46  CSICLEPFTLQDPSTVTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVE 105

Query: 95  QERSFRLNPVRNST-IFRHPTFGDFELQHLPAGADDADLEERILQHLAAAAAMGQARHSA 154
           +ER  +   + +S+ I  H +  DF  +     +  +  +E+ L+HL  AA     R   
Sbjct: 106 KERLLKTRNISSSSPISIHHSHDDFHSEE--EESQFSSFDEQFLRHLTEAA---HRRCLL 165

Query: 155 RREGHRHRSGIHGRPQLLVFSSPPNSSSTSPLPRDNREGEAASRIMVATVSP-PQPAGEE 214
           RR   +  S        LV SS P +   + L    R   A S +     +P P P    
Sbjct: 166 RRRDGQISSS-------LVSSSDPTTIHPTDLVNLYRL-SAISHVEHQNSNPCPSPGS-- 225

Query: 215 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQS 274
                                                    XXXXXXXXXXXXXXXX   
Sbjct: 226 ------------------MTPSPVSGHSSIPADSNNGSRISXXXXXXXXXXXXXXXXXXX 285

Query: 275 LSDSLKSRFNAMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMM 334
             +++KS+  A S +YK+SI+KS +G KEKL +RN S+ ++   V+RE+N GIA V+RM+
Sbjct: 286 XXEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVKELSKGVQREMNAGIAGVARMI 325

Query: 335 ERLETRDARKSSS 346
           ER++    R   S
Sbjct: 346 ERMDFSSKRFGGS 325

BLAST of Carg25977 vs. TAIR10
Match: AT4G00335.1 (RING-H2 finger B1A)

HSP 1 Score: 60.8 bits (146), Expect = 2.1e-09
Identity = 25/55 (45.45%), Postives = 35/55 (63.64%), Query Frame = 0

Query: 29  DACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMCWQPVSLKD 84
           D  +D C IC E++   +P   T C+HEFHL C+LEW +RS +CP+C + V   D
Sbjct: 133 DEEEDCCPICFEDYDVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFDD 187

BLAST of Carg25977 vs. TAIR10
Match: AT3G02290.1 (RING/U-box superfamily protein)

HSP 1 Score: 58.5 bits (140), Expect = 1.0e-08
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 0

Query: 32  DDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMC 76
           +D C  CLE +   +P  +T C H FHL C+ EW +RS  CP+C
Sbjct: 178 EDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVC 221

BLAST of Carg25977 vs. TAIR10
Match: AT5G15790.1 (RING/U-box superfamily protein)

HSP 1 Score: 58.5 bits (140), Expect = 1.0e-08
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 0

Query: 32  DDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMC 76
           +D C  CLE +   +P  +T C H FHL C+ EW +RS  CP+C
Sbjct: 179 EDVCPTCLEEYISENPKIVTKCSHHFHLSCIYEWMERSENCPVC 222

BLAST of Carg25977 vs. Swiss-Prot
Match: sp|Q9ZT42|RHF2A_ARATH (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.0e-102
Identity = 205/383 (53.52%), Postives = 242/383 (63.19%), Query Frame = 0

Query: 8   ETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQ 67
           ET  SE HLTSAAAFVEGGIQDACDDACSICLE+FC+SDPST+TSCKHE+HLQC+LEWCQ
Sbjct: 6   ETTTSEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQ 65

Query: 68  RSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQHLPAGA 127
           RSSQCPMCWQ +SLKDPTSQELLEAVEQER+FR NP RN+TIFRHPT GDFELQHLP G 
Sbjct: 66  RSSQCPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGV 125

Query: 128 DDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSSTSPLPR 187
           D+A++EERI+QHLAAAAAMG+ARH  RREGHR RS   G  Q +VFSS PN+SS      
Sbjct: 126 DNAEIEERIIQHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPXXXX 185

Query: 188 DNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 247
                         +  P    GE                                    
Sbjct: 186 XXXXXXXXXXSDTVSNLPHNALGE-------------GSHQSNTQPPTSSHPRQVSPSAS 245

Query: 248 XXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWKEKLFSRN 307
                                 LQS S+SLKSR NA+S RYK+SI+K+TR WK++LFSRN
Sbjct: 246 DSNSRPLNQSSPSEQDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRN 305

Query: 308 TSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSESNDRRTXX 367
           TSM+D+GSEV+REV+ GIATVSRMMERLETR+  + S+   S+V +N+    +N+     
Sbjct: 306 TSMADLGSEVKREVSAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAA 365

Query: 368 XXXXXXXXXXXKEGSCPAESSSS 391
                       + +C   S SS
Sbjct: 366 AGDEHSVNERGVKETCATGSGSS 375

BLAST of Carg25977 vs. Swiss-Prot
Match: sp|Q4TU14|RHF1A_ARATH (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.0e-29
Identity = 119/313 (38.02%), Postives = 161/313 (51.44%), Query Frame = 0

Query: 35  CSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVE 94
           CSICLE F   DPST+TSCKHE+HLQC++EW QRS +CP+CWQ   L+DP SQELL AVE
Sbjct: 46  CSICLEPFTLQDPSTVTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVE 105

Query: 95  QERSFRLNPVRNST-IFRHPTFGDFELQHLPAGADDADLEERILQHLAAAAAMGQARHSA 154
           +ER  +   + +S+ I  H +  DF  +     +  +  +E+ L+HL  AA     R   
Sbjct: 106 KERLLKTRNISSSSPISIHHSHDDFHSEE--EESQFSSFDEQFLRHLTEAA---HRRCLL 165

Query: 155 RREGHRHRSGIHGRPQLLVFSSPPNSSSTSPLPRDNREGEAASRIMVATVSP-PQPAGEE 214
           RR   +  S        LV SS P +   + L    R   A S +     +P P P    
Sbjct: 166 RRRDGQISSS-------LVSSSDPTTIHPTDLVNLYRL-SAISHVEHQNSNPCPSPGS-- 225

Query: 215 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQS 274
                                                    XXXXXXXXXXXXXXXX   
Sbjct: 226 ------------------MTPSPVSGHSSIPADSNNGSRISXXXXXXXXXXXXXXXXXXX 285

Query: 275 LSDSLKSRFNAMSMRYKDSIAKSTRGWKEKLFSRNTSMSDIGSEVRREVNVGIATVSRMM 334
             +++KS+  A S +YK+SI+KS +G KEKL +RN S+ ++   V+RE+N GIA V+RM+
Sbjct: 286 XXEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVKELSKGVQREMNAGIAGVARMI 325

Query: 335 ERLETRDARKSSS 346
           ER++    R   S
Sbjct: 346 ERMDFSSKRFGGS 325

BLAST of Carg25977 vs. Swiss-Prot
Match: sp|Q2HIJ8|RHB1A_ARATH (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.8e-08
Identity = 25/55 (45.45%), Postives = 35/55 (63.64%), Query Frame = 0

Query: 29  DACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMCWQPVSLKD 84
           D  +D C IC E++   +P   T C+HEFHL C+LEW +RS +CP+C + V   D
Sbjct: 133 DEEEDCCPICFEDYDVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFDD 187

BLAST of Carg25977 vs. Swiss-Prot
Match: sp|Q8LE94|RING3_ARATH (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.9e-07
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 0

Query: 32  DDACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMC 76
           +D C  CLE +   +P  +T C H FHL C+ EW +RS  CP+C
Sbjct: 178 EDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVC 221

BLAST of Carg25977 vs. Swiss-Prot
Match: sp|Q93ZF6|AIRP1_ARATH (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 7.1e-07
Identity = 21/43 (48.84%), Postives = 28/43 (65.12%), Query Frame = 0

Query: 33  DACSICLENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMC 76
           D C ICLE +   +P  +T C H+FHL C+L W +RS  CP+C
Sbjct: 102 DECPICLEEYEIDNPKLLTKCGHDFHLACILAWMERSEACPVC 144

BLAST of Carg25977 vs. TrEMBL
Match: tr|A0A1S3BPZ3|A0A1S3BPZ3_CUCME (E3 ubiquitin-protein ligase RHF2A-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491963 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 6.6e-151
Identity = 327/390 (83.85%), Postives = 336/390 (86.15%), Query Frame = 0

Query: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60
           MESPTMDETK SEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ
Sbjct: 1   MESPTMDETKKSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60

Query: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120
           CVLEWCQRSSQCPMCWQP+SLKDPTSQELLEAVEQERSFRLNP RNSTIFRHPT GDFEL
Sbjct: 61  CVLEWCQRSSQCPMCWQPISLKDPTSQELLEAVEQERSFRLNPARNSTIFRHPTLGDFEL 120

Query: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180
           QHLPAGA DADLEERILQHLAAAAAMGQARHSARRE HR+RSGIHGRPQ LVFSS PNSS
Sbjct: 121 QHLPAGASDADLEERILQHLAAAAAMGQARHSARRESHRNRSGIHGRPQFLVFSSHPNSS 180

Query: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           STSP P D+REGEAA RIMVA++SPP PAGEE          XXXXXXXXXXXXXXXXXX
Sbjct: 181 STSPPPGDDREGEAAPRIMVASISPPSPAGEE-APQSNASVSXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWK 300
           XXXXXXXXXXXXXXXX             LQS SDSLKSRFNAMSMRYKDSI KSTRGW+
Sbjct: 241 XXXXXXXXXXXXXXXXPVPNSQDRAEPSELQSFSDSLKSRFNAMSMRYKDSITKSTRGWR 300

Query: 301 EKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSES 360
           EKLFSRNTSMSDIGSEVRREVN GIATVSRMMERLETRDARKSSSPESS+VGD+NPVSES
Sbjct: 301 EKLFSRNTSMSDIGSEVRREVNAGIATVSRMMERLETRDARKSSSPESSSVGDSNPVSES 360

Query: 361 NDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           N+R               KEG  PAESSSS
Sbjct: 361 NNRSMPENGGNSSLTDTNKEGPRPAESSSS 389

BLAST of Carg25977 vs. TrEMBL
Match: tr|A0A1S3BPM2|A0A1S3BPM2_CUCME (E3 ubiquitin-protein ligase RHF2A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491963 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 5.6e-150
Identity = 325/390 (83.33%), Postives = 335/390 (85.90%), Query Frame = 0

Query: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60
           + SPTMDETK SEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ
Sbjct: 4   LNSPTMDETKKSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 63

Query: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120
           CVLEWCQRSSQCPMCWQP+SLKDPTSQELLEAVEQERSFRLNP RNSTIFRHPT GDFEL
Sbjct: 64  CVLEWCQRSSQCPMCWQPISLKDPTSQELLEAVEQERSFRLNPARNSTIFRHPTLGDFEL 123

Query: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180
           QHLPAGA DADLEERILQHLAAAAAMGQARHSARRE HR+RSGIHGRPQ LVFSS PNSS
Sbjct: 124 QHLPAGASDADLEERILQHLAAAAAMGQARHSARRESHRNRSGIHGRPQFLVFSSHPNSS 183

Query: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           STSP P D+REGEAA RIMVA++SPP PAGEE          XXXXXXXXXXXXXXXXXX
Sbjct: 184 STSPPPGDDREGEAAPRIMVASISPPSPAGEE-APQSNASVSXXXXXXXXXXXXXXXXXX 243

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWK 300
           XXXXXXXXXXXXXXXX             LQS SDSLKSRFNAMSMRYKDSI KSTRGW+
Sbjct: 244 XXXXXXXXXXXXXXXXPVPNSQDRAEPSELQSFSDSLKSRFNAMSMRYKDSITKSTRGWR 303

Query: 301 EKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSES 360
           EKLFSRNTSMSDIGSEVRREVN GIATVSRMMERLETRDARKSSSPESS+VGD+NPVSES
Sbjct: 304 EKLFSRNTSMSDIGSEVRREVNAGIATVSRMMERLETRDARKSSSPESSSVGDSNPVSES 363

Query: 361 NDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           N+R               KEG  PAESSSS
Sbjct: 364 NNRSMPENGGNSSLTDTNKEGPRPAESSSS 392

BLAST of Carg25977 vs. TrEMBL
Match: tr|A0A1S3BP10|A0A1S3BP10_CUCME (E3 ubiquitin-protein ligase RHF2A-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103491963 PE=4 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 5.2e-148
Identity = 322/385 (83.64%), Postives = 331/385 (85.97%), Query Frame = 0

Query: 6   MDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEW 65
           MDETK SEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEW
Sbjct: 1   MDETKKSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEW 60

Query: 66  CQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQHLPA 125
           CQRSSQCPMCWQP+SLKDPTSQELLEAVEQERSFRLNP RNSTIFRHPT GDFELQHLPA
Sbjct: 61  CQRSSQCPMCWQPISLKDPTSQELLEAVEQERSFRLNPARNSTIFRHPTLGDFELQHLPA 120

Query: 126 GADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSSTSPL 185
           GA DADLEERILQHLAAAAAMGQARHSARRE HR+RSGIHGRPQ LVFSS PNSSSTSP 
Sbjct: 121 GASDADLEERILQHLAAAAAMGQARHSARRESHRNRSGIHGRPQFLVFSSHPNSSSTSPP 180

Query: 186 PRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 245
           P D+REGEAA RIMVA++SPP PAGEE          XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 181 PGDDREGEAAPRIMVASISPPSPAGEE-APQSNASVSXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 246 XXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWKEKLFS 305
           XXXXXXXXXXX             LQS SDSLKSRFNAMSMRYKDSI KSTRGW+EKLFS
Sbjct: 241 XXXXXXXXXXXPVPNSQDRAEPSELQSFSDSLKSRFNAMSMRYKDSITKSTRGWREKLFS 300

Query: 306 RNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSESNDRRT 365
           RNTSMSDIGSEVRREVN GIATVSRMMERLETRDARKSSSPESS+VGD+NPVSESN+R  
Sbjct: 301 RNTSMSDIGSEVRREVNAGIATVSRMMERLETRDARKSSSPESSSVGDSNPVSESNNRSM 360

Query: 366 XXXXXXXXXXXXXKEGSCPAESSSS 391
                        KEG  PAESSSS
Sbjct: 361 PENGGNSSLTDTNKEGPRPAESSSS 384

BLAST of Carg25977 vs. TrEMBL
Match: tr|A0A0A0M0B5|A0A0A0M0B5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629730 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 9.9e-147
Identity = 334/390 (85.64%), Postives = 344/390 (88.21%), Query Frame = 0

Query: 1   MESPTMDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60
           MESPTMDETK SEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ
Sbjct: 1   MESPTMDETKKSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQ 60

Query: 61  CVLEWCQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFEL 120
           CVLEWCQRSSQCPMCWQ +SLKDPTSQELLEAVEQERSFRLNP RNSTIFRHPT GDFEL
Sbjct: 61  CVLEWCQRSSQCPMCWQSISLKDPTSQELLEAVEQERSFRLNPARNSTIFRHPTLGDFEL 120

Query: 121 QHLPAGADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSS 180
           QHLPAGA+DADLEERILQHLAAAAAMGQARHSARRE HR+RSGIHGRPQ LVFSS PNSS
Sbjct: 121 QHLPAGANDADLEERILQHLAAAAAMGQARHSARRESHRNRSGIHGRPQFLVFSSHPNSS 180

Query: 181 STSPLPRDNREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           STSP   D+REGEAA RIMVA++SPP PAGEE          XXXXXXXXXXXXXXXXXX
Sbjct: 181 STSPSSGDDREGEAAPRIMVASISPPPPAGEE-APQSNASVSXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWK 300
           XXXXXXXXXXXXXXX              LQS SDSLKSRFNAMSMRYKDSI KST+GWK
Sbjct: 241 XXXXXXXXXXXXXXXQPVPNNQDRAGPSELQSFSDSLKSRFNAMSMRYKDSITKSTKGWK 300

Query: 301 EKLFSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSES 360
           EKLFSRNTSMSDIGSEVRREVN GIATVSRMMERLETRDARKSSSPESS+VG N+PVSES
Sbjct: 301 EKLFSRNTSMSDIGSEVRREVNAGIATVSRMMERLETRDARKSSSPESSSVGGNSPVSES 360

Query: 361 NDRRTXXXXXXXXXXXXXKEGSCPAESSSS 391
           N+R  XXXXXXXXXXXXX EG   AESSSS
Sbjct: 361 NNRSMXXXXXXXXXXXXXXEGPRSAESSSS 389

BLAST of Carg25977 vs. TrEMBL
Match: tr|A0A1R3G0M0|A0A1R3G0M0_COCAP (Zinc finger, RING-type OS=Corchorus capsularis OX=210143 GN=CCACVL1_29687 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 4.9e-114
Identity = 234/386 (60.62%), Postives = 265/386 (68.65%), Query Frame = 0

Query: 6   MDETKNSEAHLTSAAAFVEGGIQDACDDACSICLENFCDSDPSTMTSCKHEFHLQCVLEW 65
           M+ETK SE HLTSAAAFVEGGIQDACDDACSICLE FC+SDPST+TSCKHEFHLQC+LEW
Sbjct: 1   MEETK-SEVHLTSAAAFVEGGIQDACDDACSICLEAFCESDPSTVTSCKHEFHLQCILEW 60

Query: 66  CQRSSQCPMCWQPVSLKDPTSQELLEAVEQERSFRLNPVRNSTIFRHPTFGDFELQHLPA 125
           CQRSSQCPMCWQP+SLKDPTSQELLEAVE+ERSFR NP RN+TIF HPT GDFELQHLP 
Sbjct: 61  CQRSSQCPMCWQPISLKDPTSQELLEAVERERSFRFNPSRNATIFHHPTLGDFELQHLPV 120

Query: 126 GADDADLEERILQHLAAAAAMGQARHSARREGHRHRSGIHGRPQLLVFSSPPNSSSTSPL 185
           GA++A+LEERI+QHLAAAAAMG+ARH ARREG R+RS   GRPQ LVFS+ PN+ STSP+
Sbjct: 121 GANEAELEERIIQHLAAAAAMGRARHIARREGLRNRSSAQGRPQFLVFSTHPNAPSTSPI 180

Query: 186 PRD--NREGEAASRIMVATVSPPQPAGEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 245
                 REGE A  I + T S P     E                               
Sbjct: 181 SSSPTQREGEPAHAITLGTPSSPARTAGEESSASFTPVTSAHADQQPALASGSSVLSVND 240

Query: 246 XXXXXXXXXXXXXXXXXXXXXXXXXXLQSLSDSLKSRFNAMSMRYKDSIAKSTRGWKEKL 305
                                      QS S+SLKSRFNA+SMRYK+SI+KSTRGWKE+L
Sbjct: 241 QGNSLNNRRSPNQSSPNSQDRAGPSEFQSFSESLKSRFNAVSMRYKESISKSTRGWKERL 300

Query: 306 FSRNTSMSDIGSEVRREVNVGIATVSRMMERLETRDARKSSSPESSNVGDNNPVSESNDR 365
           FSRNTSM+D+GSEVRREVN GIATVSRMMERLETRD R S+S  SS++ D +  SESN+R
Sbjct: 301 FSRNTSMADLGSEVRREVNAGIATVSRMMERLETRDNRTSTSSVSSSLEDGSN-SESNNR 360

Query: 366 RTXXXXXXXXXXXXXKEGSCPAESSS 390
           +               + SC A S S
Sbjct: 361 QISDACGEAHLTDNSTQASCAASSGS 384

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022925357.15.8e-16799.23E3 ubiquitin-protein ligase RHF2A-like isoform X3 [Cucurbita moschata][more]
XP_022925353.11.2e-16485.78E3 ubiquitin-protein ligase RHF2A-like isoform X2 [Cucurbita moschata][more]
XP_022925348.11.3e-16385.29E3 ubiquitin-protein ligase RHF2A-like isoform X1 [Cucurbita moschata][more]
XP_022974093.11.1e-16281.62E3 ubiquitin-protein ligase RHF2A-like isoform X2 [Cucurbita maxima][more]
XP_022974084.11.6e-16181.53E3 ubiquitin-protein ligase RHF2A-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G22000.24.2e-10353.40RING-H2 group F2A[more]
AT4G14220.11.6e-3038.02RING-H2 group F1A[more]
AT4G00335.12.1e-0945.45RING-H2 finger B1A[more]
AT3G02290.11.0e-0845.45RING/U-box superfamily protein[more]
AT5G15790.11.0e-0845.45RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9ZT42|RHF2A_ARATH2.0e-10253.52E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ... [more]
sp|Q4TU14|RHF1A_ARATH3.0e-2938.02E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ... [more]
sp|Q2HIJ8|RHB1A_ARATH3.8e-0845.45Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
sp|Q8LE94|RING3_ARATH1.9e-0745.45E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022... [more]
sp|Q93ZF6|AIRP1_ARATH7.1e-0748.84E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BPZ3|A0A1S3BPZ3_CUCME6.6e-15183.85E3 ubiquitin-protein ligase RHF2A-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
tr|A0A1S3BPM2|A0A1S3BPM2_CUCME5.6e-15083.33E3 ubiquitin-protein ligase RHF2A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
tr|A0A1S3BP10|A0A1S3BP10_CUCME5.2e-14883.64E3 ubiquitin-protein ligase RHF2A-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC... [more]
tr|A0A0A0M0B5|A0A0A0M0B5_CUCSA9.9e-14785.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629730 PE=4 SV=1[more]
tr|A0A1R3G0M0|A0A1R3G0M0_COCAP4.9e-11460.62Zinc finger, RING-type OS=Corchorus capsularis OX=210143 GN=CCACVL1_29687 PE=4 S... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25977-RACarg25977-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 35..75
e-value: 3.9E-5
score: 33.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 34..75
e-value: 8.8E-9
score: 35.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 35..75
score: 12.194
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 6..109
e-value: 1.1E-13
score: 52.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 332..390
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 344..390
NoneNo IPR availablePANTHERPTHR44204:SF2E3 UBIQUITIN-PROTEIN LIGASE RHF2Acoord: 14..389
NoneNo IPR availablePANTHERPTHR44204FAMILY NOT NAMEDcoord: 14..389
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 28..83