Carg25099 (gene) Silver-seed gourd

Overview
NameCarg25099
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionmembrane steroid-binding protein 2
LocationCucurbita_argyrosperma_scaffold_072 : 469537 .. 472568 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAAAAAGCTGCTGCCCTTTTCCTCTCTCATTCTCCTCAGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCTGCCATGGCTCTGCAAGTCTGGGAGACGTTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCGTCGACCTTCTTCACAGTCTTGGCTCTTGGATTGGCCTTTTACTATCTCATTTCGAGTTTCTTTGGTCCTTCCGACTACGGAACACATACTAGGGACTTAGGGGAGCAGCTCCAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAGGTTTCTGAGGATGAGTTGAAGCAATACGATGGCTCCGATCCTGAGAAGCCTCTGCTCATGGCTATCAAGGGTCAGATCTACGATGTTTCTCAGAGCAGGTCGGTTGAGAATTTTCTTTTATTGAAAAATTTCCTCTCTGTTTTAGCTATTTAGTGATTGGATGATCGTGATGTGTTTTTATTCAGTTTCAATTACTTCTTGAGTCAATGTTTTTGTTGAGTTTCGTGGTGGTAGGTGGAATGTTAGCCGGTTTTAGGTTTACAAAAGCTTTTTATATTTTATTTCAGTTTTTTGAAGTAATTGATTTCGTGCTTCGTTTTGCCTTCTACAGCTCTCCTTTTTCGTTCAAAGAGCTTACTGATTCCCGCAAAATGAAACTTGATATGATCCTACTGTATTGAACCGCATCGATTTTTTTTTTTTTTGGCTATTTCTAATTCGTCCTCTCTTCCTGTTTCTTTTGTTAAGAATCATTCATTGTCCACTATACACCCCCTATCTTTTATGGTGTTCTATGTTTGTCATAATATATGCTACGACAACCAAAGAAGAACTTATTGAATTTTTGAGTTCTGGACTTCAATTCAAACTTTTAATCTTTTTCGTGTTGGACCAGAAAACCTCTCAAAATAGATTGGTGAGATTAATCTTCTTGTTTTTGTGCATCTGAATTTTCATTTTGATGTTTATTGTTCCTTCTTATAGCTGTAATTTGCTACATATCTGGTTTTGAAAATATTTAATTGGGACTGTCCTTTTTTGCTATCAAGTTACGGCCATGGTATTTCCAGCATATAGAATTCTTATAGCATGTGGAGTTCACAAAGGCAAGAGTTTGATGTTCCACGAGTTATTGTGAAAACTTGGCTTGTAAAGGGACCATAGAGACCGATTCTTCTTTTATTTGGGGCTGAAAAAGGTTCTGTTAGATGTAAAATCACTTTTAAACGAGCTAATGATTTCAAATCACTTTTTACAAACTTATGATTTGAAAGAGAATAAGTTTGAGAGAAAACAATGTAGAAAGTAGTTATTCTTATGCGCTAACAATTAGAAAGTTGCTTTTCCAAAGACTTTATAAGAACAGGTCCTTTGTTTTGTGGTTAGGGTATACTTTTGAACCATTCTTTGCATGATGTGTTGGTTGGAAATGACCCACGATCTAAATATGTCAAAAAAAATAAAGGGAGCAAAAAGAGGAGAGAGCTTTTGGTACAAGGAACCTTAATCACTTAGCTTTTCAATTTTCTTTGTTGTTGTGAAAAAAACATACATCTTAGGAGTCTTTGTTGTTGTGAAAAAAACATACATCTTAGGAGTACTTACAGTGGAGAAAGCACTAATTTGCTTCCTAAACTTTCTCCTCGCACTAACAATCTTCCTAGATAAATCATAAAACTTGGTCCACAATTTGGCTGATTCATTTTGATTAAGCTTCCTTCCAAAGGAGTACTTACCGTGGAGAAAGCACTAATTTGCTTCCTAAACTTTCTCCTCGCACTAACAATCTTCCTAGATAAATCATAAAACTTGGTCCACAATTTGGCTAATTCATTTTGATTAAGCTTCCTTCCTATTAATTTGGAAGTTCTTCTCAGATACAAGGAAATCATTCAGTTCCAAAGTCAAAGATTGTAATTCTCGAACCAGCAATTGAAAAGATTCTGGAGTATCCTCAAGTTCAGTATTGTTCCTCCAATGATCATAGTACCCAAGTAACTACTACCAATTCTCCTATATTGGTCCATACGTTAGTAACTTATTCTACTAAATCCACAAACAATTCTCTTAAAGTTACTCCATAACTTAGTGACTTATTCTACTAAGTCAAGTATATAAGCTCTTAGTGTTATTTACTACACTAAGGATGGTAGAAACGAAGATCTGTTTATATTTAGTGACTTATTCTACTAAGTCAAGTTTATAAACTCACAATGTTATTTACTACCCTAAGGTTGTAGACACGAAGATTTGTTTATGTCTCCTGTTTCTTTTTCGTGTCTTGTTCTCTCGTGTGTGCGTACTCGTAAAGGAAAACTAAAATTGAAGAAAATATACGCCTATATATATTGCTCCAATCTTGACCCTCTTTGGTTGCAGGATGTTCTATGGACCTGGAGGGCCGTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCATTTGAAGAGAAAGATCTGACAGGGGATATCTCTGGTCTTGGGCCATTTGAACTGGAAGCCTTACAGGACTGGGAATACAAATTCATGAGTAAATATGTTAAAGTTGGGACAATCAAGAAGCCAGTTGCTGAAGGTGATACTGCGCCGGCTGCTGGTGAGCCTGCAGAATCAAGCACAGCAGAAGTTAGCAGCAGCAGCAGCAAGCCTGCTGAAGACAGTCCCTCGGAATCTCATGCTGATAAAGCCGCCGAGGAAACCCCTGCTGCTGCAAAAAACGTTGAATAATATTTTTGTGAGCGGAGAAGCCAAAAGAGAACGATGTGTACGTGTGGTCATGTTTCAGTAATCTTGTTTTATAGATAAGTGAAGTGTAGGTGTGTAGGAAGGTATTACAGATGGAGTTAAGACTTAGAGTTTATGTTACTCTTGTTCAACAAACATCTGATAATAAGATAAGTCTTTGAATTTGGAAATAGGACTCAGGTCTCTCTTTCCCCAATTTGTTTGATCTGATTGGATTGAAACTGAAAGTATGAACTAATGAATAAAAATAAATAGGGGATTAGCAATTC

mRNA sequence

ACAAAAAGCTGCTGCCCTTTTCCTCTCTCATTCTCCTCAGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCTGCCATGGCTCTGCAAGTCTGGGAGACGTTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCGTCGACCTTCTTCACAGTCTTGGCTCTTGGATTGGCCTTTTACTATCTCATTTCGAGTTTCTTTGGTCCTTCCGACTACGGAACACATACTAGGGACTTAGGGGAGCAGCTCCAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAGGTTTCTGAGGATGAGTTGAAGCAATACGATGGCTCCGATCCTGAGAAGCCTCTGCTCATGGCTATCAAGGGTCAGATCTACGATGTTTCTCAGAGCAGGATGTTCTATGGACCTGGAGGGCCGTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCATTTGAAGAGAAAGATCTGACAGGGGATATCTCTGGTCTTGGGCCATTTGAACTGGAAGCCTTACAGGACTGGGAATACAAATTCATGAGTAAATATGTTAAAGTTGGGACAATCAAGAAGCCAGTTGCTGAAGGTGATACTGCGCCGGCTGCTGGTGAGCCTGCAGAATCAAGCACAGCAGAAGTTAGCAGCAGCAGCAGCAAGCCTGCTGAAGACAGTCCCTCGGAATCTCATGCTGATAAAGCCGCCGAGGAAACCCCTGCTGCTGCAAAAAACGTTGAATAATATTTTTGTGAGCGGAGAAGCCAAAAGAGAACGATGTGTACGTGTGGTCATGTTTCAGTAATCTTGTTTTATAGATAAGTGAAGTGTAGGTGTGTAGGAAGGTATTACAGATGGAGTTAAGACTTAGAGTTTATGTTACTCTTGTTCAACAAACATCTGATAATAAGATAAGTCTTTGAATTTGGAAATAGGACTCAGGTCTCTCTTTCCCCAATTTGTTTGATCTGATTGGATTGAAACTGAAAGTATGAACTAATGAATAAAAATAAATAGGGGATTAGCAATTC

Coding sequence (CDS)

ATGGCTCTGCAAGTCTGGGAGACGTTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCGTCGACCTTCTTCACAGTCTTGGCTCTTGGATTGGCCTTTTACTATCTCATTTCGAGTTTCTTTGGTCCTTCCGACTACGGAACACATACTAGGGACTTAGGGGAGCAGCTCCAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAGGTTTCTGAGGATGAGTTGAAGCAATACGATGGCTCCGATCCTGAGAAGCCTCTGCTCATGGCTATCAAGGGTCAGATCTACGATGTTTCTCAGAGCAGGATGTTCTATGGACCTGGAGGGCCGTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCATTTGAAGAGAAAGATCTGACAGGGGATATCTCTGGTCTTGGGCCATTTGAACTGGAAGCCTTACAGGACTGGGAATACAAATTCATGAGTAAATATGTTAAAGTTGGGACAATCAAGAAGCCAGTTGCTGAAGGTGATACTGCGCCGGCTGCTGGTGAGCCTGCAGAATCAAGCACAGCAGAAGTTAGCAGCAGCAGCAGCAAGCCTGCTGAAGACAGTCCCTCGGAATCTCATGCTGATAAAGCCGCCGAGGAAACCCCTGCTGCTGCAAAAAACGTTGAATAA

Protein sequence

MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQPLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGEPAESSTAEVSSSSSKPAEDSPSESHADKAAEETPAAAKNVE
Homology
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BLAST of Carg25099 vs. NCBI nr
Match: XP_022952639.1 (membrane steroid-binding protein 2-like [Cucurbita moschata] >XP_022952640.1 membrane steroid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 372.9 bits (956), Expect = 7.8e-100
Identity = 218/221 (98.64%), Postives = 218/221 (98.64%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGD APAAG 
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGX 180

Query: 181 PXXXXXXXXXXXXXXXXXXXXXXXXXXXAAEETPAAAKNVE 222
            XXXXXXXXXXXXXXXXXXXXXXXXXXXAAEETPAAAKNVE
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXAAEETPAAAKNVE 221

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BLAST of Carg25099 vs. NCBI nr
Match: XP_023554188.1 (membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 371.3 bits (952), Expect = 2.3e-99
Identity = 214/221 (96.83%), Postives = 215/221 (97.29%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVA+GD  PAAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180

Query: 181 PXXXXXXXXXXXXXXXXXXXXXXXXXXXAAEETPAAAKNVE 222
           P  XXXXXXXXXXXXXXXXXXXXXXX  AAEETPAAAKNVE
Sbjct: 181 P-AXXXXXXXXXXXXXXXXXXXXXXXDRAAEETPAAAKNVE 220

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BLAST of Carg25099 vs. NCBI nr
Match: XP_022971922.1 (membrane steroid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 360.1 bits (923), Expect = 5.2e-96
Identity = 177/179 (98.88%), Postives = 178/179 (99.44%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQIGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAG 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGD APAAG
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAG 179

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BLAST of Carg25099 vs. NCBI nr
Match: XP_022957722.1 (membrane steroid-binding protein 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 330.5 bits (846), Expect = 4.4e-87
Identity = 164/182 (90.11%), Postives = 170/182 (93.41%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFT +A GLAFY+LISS F PSD  THTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTAVAFGLAFYFLISSLFAPSDNRTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGE+SE+ELKQYDGSD EKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEISEEELKQYDGSDSEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAE-GDTAPAAG 180
           RALAKMSFEEKDLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGT+KKPVAE GD AP   
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTVKKPVAEGGDAAPVVV 180

Query: 181 EP 182
           EP
Sbjct: 181 EP 182

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BLAST of Carg25099 vs. NCBI nr
Match: XP_023534575.1 (membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.5 bits (846), Expect = 4.4e-87
Identity = 162/175 (92.57%), Postives = 168/175 (96.00%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFT +ALGLAFYYLISSFF PSD  THTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTAVALGLAFYYLISSFFAPSDNRTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SE+ELKQYDGSD EKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEEELKQYDGSDSEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTA 176
           RALAKMSFEEKDLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGT+KKPVAEG  A
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTVKKPVAEGGDA 175

[Close]
BLAST of Carg25099 vs. TAIR10
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3)

HSP 1 Score: 261.9 bits (668), Expect = 3.5e-70
Identity = 130/181 (71.82%), Postives = 148/181 (81.77%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFF-GPSDYGTHTRDLGEQLQ 60
           M  Q+WETLKE I AYTGLSP+ FFTVLAL  A Y ++S FF  P  +   + ++  Q +
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GE++E+ELK YDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAG 180
           SRALAKMSFE++DLTGDISGLG FELEALQDWEYKFMSKYVKVGTI+K   EG  +    
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPS 180

[Close]
BLAST of Carg25099 vs. TAIR10
Match: AT5G52240.1 (membrane steroid binding protein 1)

HSP 1 Score: 254.6 bits (649), Expect = 5.6e-68
Identity = 131/186 (70.43%), Postives = 149/186 (80.11%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  A Y +IS +F     D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGE++E+ELKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTA 180
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGT+K   +E +TA
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPETA 180

Query: 181 PAAGEP 182
            +  EP
Sbjct: 181 -SVSEP 185

[Close]
BLAST of Carg25099 vs. TAIR10
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2)

HSP 1 Score: 108.6 bits (270), Expect = 4.9e-24
Identity = 48/97 (49.48%), Postives = 69/97 (71.13%), Query Frame = 0 

Query: 69  EVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 128
           E + ++L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 129 EEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTI 166
            E+D++  + GL   E+  L DWE KF +KY  VG +
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

[Close]
BLAST of Carg25099 vs. TAIR10
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4)

HSP 1 Score: 78.6 bits (192), Expect = 5.5e-15
Identity = 36/95 (37.89%), Postives = 55/95 (57.89%), Query Frame = 0 

Query: 71  SEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEE 130
           S +EL  Y+G+D   P+L+ I G ++DV++ +  YG GG Y  FAG+DASRA    +F  
Sbjct: 43  SAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTG 102

Query: 131 KDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTI 166
             LT  + GL   E++++ DW   +   Y  VG +
Sbjct: 103 DGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKL 137

[Close]
BLAST of Carg25099 vs. Swiss-Prot
Match: sp|Q9M2Z4|MSBP2_ARATH (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 6.3e-69
Identity = 130/181 (71.82%), Postives = 148/181 (81.77%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFF-GPSDYGTHTRDLGEQLQ 60
           M  Q+WETLKE I AYTGLSP+ FFTVLAL  A Y ++S FF  P  +   + ++  Q +
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GE++E+ELK YDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAG 180
           SRALAKMSFE++DLTGDISGLG FELEALQDWEYKFMSKYVKVGTI+K   EG  +    
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPS 180

[Close]
BLAST of Carg25099 vs. Swiss-Prot
Match: sp|Q9XFM6|MSBP1_ARATH (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 1.0e-66
Identity = 131/186 (70.43%), Postives = 149/186 (80.11%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  A Y +IS +F     D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGE++E+ELKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTA 180
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGT+K   +E +TA
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPETA 180

Query: 181 PAAGEP 182
            +  EP
Sbjct: 181 -SVSEP 185

[Close]
BLAST of Carg25099 vs. Swiss-Prot
Match: sp|Q9FVZ7|MSBP1_ORYSJ (Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP1 PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 5.2e-63
Identity = 121/175 (69.14%), Postives = 138/175 (78.86%), Query Frame = 0 

Query: 4   QVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFF-GPSDYGTHTRDLGEQLQPLP 63
           ++WETLK+AIVAYTGLSP+ FFT +A   A Y+++S  F G                   
Sbjct: 7   ELWETLKQAIVAYTGLSPAAFFTAVAAAAALYHVVSGIFAGXXXXXXXXXXXXXXXXXXX 66

Query: 64  PPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRA 123
             VQ+GEVSE+EL+QYDGSDP+KPLLMAIKGQIYDV+QSRMFYGPGGPYALFAGKDASRA
Sbjct: 67  XXVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDASRA 126

Query: 124 LAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPA 178
           LAKMSFE +DLTGDISGLGPFEL+ALQDWEYKFM KYVKVGT+KK V   D AP+
Sbjct: 127 LAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMGKYVKVGTVKKTVPVEDGAPS 181

[Close]
BLAST of Carg25099 vs. Swiss-Prot
Match: sp|Q9FVZ9|MSBP2_ORYSJ (Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP2 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-46
Identity = 95/119 (79.83%), Postives = 102/119 (85.71%), Query Frame = 0 

Query: 64  PVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRAL 123
           PVQVGE++ +EL QYDGSDPEKPLLMAIKGQIYDVSQSR+FYGPGGPYALFAGKDASRAL
Sbjct: 115 PVQVGEITAEELLQYDGSDPEKPLLMAIKGQIYDVSQSRLFYGPGGPYALFAGKDASRAL 174

Query: 124 AKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAE--GDTAPAAGE 181
           AKMSFE +DLT DISGL   EL ALQDWEYKF SKYVKVGTIKK + E  GD+   A E
Sbjct: 175 AKMSFEPQDLTDDISGLSLLELSALQDWEYKFSSKYVKVGTIKKVLVEQGGDSTADAIE 233

[Close]
BLAST of Carg25099 vs. Swiss-Prot
Match: sp|Q9SK39|SBP3_ARATH (Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.9e-23
Identity = 48/97 (49.48%), Postives = 69/97 (71.13%), Query Frame = 0 

Query: 69  EVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 128
           E + ++L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 129 EEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTI 166
            E+D++  + GL   E+  L DWE KF +KY  VG +
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

[Close]
BLAST of Carg25099 vs. TrEMBL
Match: tr|A0A0A0LIG2|A0A0A0LIG2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G915110 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.5e-83
Identity = 159/181 (87.85%), Postives = 168/181 (92.82%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVW+TLKEAI+AYTGLSPSTFFTVLALGLA YYLISSFF PSDYGTH RDL +Q+ P
Sbjct: 1   MALQVWDTLKEAILAYTGLSPSTFFTVLALGLAIYYLISSFFAPSDYGTHPRDL-DQIHP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SED+LKQYDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGE 180
           RALAKMSFEEKDLTGDISGLGP ELE LQDWEYKFMSKYVKVGT+K  VAEGD   AAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPSELEMLQDWEYKFMSKYVKVGTVKTSVAEGDA--AAGE 178

Query: 181 P 182
           P
Sbjct: 181 P 178

[Close]
BLAST of Carg25099 vs. TrEMBL
Match: tr|A0A1S3CQ79|A0A1S3CQ79_CUCME (membrane steroid-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103503452 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.7e-82
Identity = 160/182 (87.91%), Postives = 167/182 (91.76%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPS-DYGTHTRDLGEQLQ 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALG A YYLISSFF PS DYGTH RDL EQ+ 
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGFAIYYLISSFFAPSADYGTHARDL-EQIH 60

Query: 61  PLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GE+SED+LKQYDG+D +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEISEDDLKQYDGTDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAG 180
           SRALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGT+KK VAEGD   AAG
Sbjct: 121 SRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKTVAEGDA--AAG 179

Query: 181 EP 182
           EP
Sbjct: 181 EP 179

[Close]
BLAST of Carg25099 vs. TrEMBL
Match: tr|A0A2C9UI66|A0A2C9UI66_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_14G026800 PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 3.5e-80
Identity = 149/181 (82.32%), Postives = 165/181 (91.16%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQ+WETLKEAI AYTGLSP+TFFTVLALGLA YY+IS FFG S+     R + EQ+QP
Sbjct: 1   MALQLWETLKEAITAYTGLSPATFFTVLALGLAVYYVISEFFGSSNDHPRPRSVEEQIQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE++E+ELKQYDG+DP+KPLLMAIKGQIYD+SQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEITEEELKQYDGTDPKKPLLMAIKGQIYDISQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGE 180
           RALAKMSFE+KDLTGDISGLGPFELE+LQDWEYKFMSKYVKVGTIKKPV   D A +AGE
Sbjct: 121 RALAKMSFEDKDLTGDISGLGPFELESLQDWEYKFMSKYVKVGTIKKPVPVTDEATSAGE 180

Query: 181 P 182
           P
Sbjct: 181 P 181

[Close]
BLAST of Carg25099 vs. TrEMBL
Match: tr|A0A218WRZ4|A0A218WRZ4_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr021789 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.2e-79
Identity = 146/181 (80.66%), Postives = 162/181 (89.50%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQ+WETLKEAI+ YTGLSP+TF  VLALGLA YY ++  FGPSD+   +RD GE+ QP
Sbjct: 1   MALQLWETLKEAIITYTGLSPATFLAVLALGLAVYYAVTGLFGPSDHHPRSRDYGEETQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GEV+E+ELKQYDG+DP+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEVTEEELKQYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGTIKKPV   +   +A E
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKKPVPVTEEGSSASE 180

Query: 181 P 182
           P
Sbjct: 181 P 181

[Close]
BLAST of Carg25099 vs. TrEMBL
Match: tr|A0A2C9VQV2|A0A2C9VQV2_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_06G146300 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.9e-79
Identity = 149/181 (82.32%), Postives = 161/181 (88.95%), Query Frame = 0 

Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQ+W TLKEAI  YTGLSP+TFFTVLALGLA YYLIS FFG SD     R + EQ+QP
Sbjct: 1   MALQLWGTLKEAITVYTGLSPATFFTVLALGLAVYYLISEFFGSSDNHPRPRSVEEQMQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SE+ELKQYDGSDP+KPLLMAIKGQIYD+SQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEEELKQYDGSDPKKPLLMAIKGQIYDISQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGE 180
           RALAKMSFE+KDLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGTIK PV     A +AGE
Sbjct: 121 RALAKMSFEDKDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKTPVPATGEATSAGE 180

Query: 181 P 182
           P
Sbjct: 181 P 181

The following BLAST results are available for this feature:
BLAST of Carg25099 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022952639.17.8e-10098.64membrane steroid-binding protein 2-like [Cucurbita moschata] >XP_022952640.1 mem... [more]
XP_023554188.12.3e-9996.83membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo][more]
XP_022971922.15.2e-9698.88membrane steroid-binding protein 2-like [Cucurbita maxima][more]
XP_022957722.14.4e-8790.11membrane steroid-binding protein 1-like isoform X1 [Cucurbita moschata][more]
XP_023534575.14.4e-8792.57membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo][more]
BLAST of Carg25099 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G48890.13.5e-7071.82membrane-associated progesterone binding protein 3[more]
AT5G52240.15.6e-6870.43membrane steroid binding protein 1[more]
AT2G24940.14.9e-2449.48membrane-associated progesterone binding protein 2[more]
AT4G14965.15.5e-1537.89membrane-associated progesterone binding protein 4[more]
BLAST of Carg25099 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9M2Z4|MSBP2_ARATH6.3e-6971.82Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1... [more]
sp|Q9XFM6|MSBP1_ARATH1.0e-6670.43Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1... [more]
sp|Q9FVZ7|MSBP1_ORYSJ5.2e-6369.14Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
sp|Q9FVZ9|MSBP2_ORYSJ1.2e-4679.83Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
sp|Q9SK39|SBP3_ARATH8.9e-2349.48Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 S... [more]
BLAST of Carg25099 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LIG2|A0A0A0LIG2_CUCSA1.5e-8387.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G915110 PE=3 SV=1[more]
tr|A0A1S3CQ79|A0A1S3CQ79_CUCME1.7e-8287.91membrane steroid-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103503452 PE=3 ... [more]
tr|A0A2C9UI66|A0A2C9UI66_MANES3.5e-8082.32Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_14G026800 PE=3 SV=... [more]
tr|A0A218WRZ4|A0A218WRZ4_PUNGR2.2e-7980.66Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr021789 PE=3 SV=1[more]
tr|A0A2C9VQV2|A0A2C9VQV2_MANES2.9e-7982.32Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_06G146300 PE=3 SV=... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR036400Cyt_B5-like_heme/steroid_sf
IPR001199Cyt_B5-like_heme/steroid-bd
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25099-RACarg25099-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 70..166
e-value: 3.9E-24
score: 96.2
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 72..165
e-value: 1.8E-14
score: 53.5
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3DG3DSA:3.10.120.10coord: 68..166
e-value: 1.3E-35
score: 123.6
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILYSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 61..166
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..199
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 200..214
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..221
NoneNo IPR availablePANTHERPTHR10281:SF45MEMBRANE STEROID-BINDING PROTEIN 2coord: 1..187
NoneNo IPR availablePANTHERPTHR10281MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATEDcoord: 1..187