Carg19550 (gene) Silver-seed gourd

NameCarg19550
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRING-type E3 ubiquitin transferase
LocationCucurbita_argyrosperma_scaffold_104 : 410756 .. 412816 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTTTGCCCCATCTCTCTCCAAATCATGAAAGACCCCGTCACGGTCGCTTCCGGCATAACCTATGACCGCAAAAGCATCGAGAAATGGTTGTTTTCAGACGGACACAAGACATGCCCCGTCACACAGGTTGTCTTATCGAGCTTCGACATCACTCCCAACATTACTCTAAGACGAGTCATTCAAGCTTGGTGTACTGTCAATGCTTGCAAGGGCATCGAGAGGATTCCGACACCTAAGCCACCGGTCGATTGTAAACAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTCCGTTCTCGCAACAGAATTGTCTCCGACGACTCCGATCTATTGCAGCGGCGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCCGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTTAGAAGGTATTATTACTATAATAAATTCAAAATGTTAAAAAGTCATATATATATATATATATATATATATATATATATATATATAATTATTTGATTTTTTTTTTTTAATTTTAAGCTTATTAAAGTATTTAGAAAAAAAAAAGAAAAAAGAAAGAAAAGATAGCTTTTTTTTTTAAAAAAAATAATAATAAAGGATTCATTTTTTATTTTTTTTAAAATTAACTTTAAAAAATTTTAATTTTCAATATTACCTCGAAACAATTCAAGAAATTTAAAATTTCTTACAATTAATTTTAATTTTTTTAAAAAATTAAAAATTTCTTACACGCTATCTAATTATCTAACCCGAATTATTAAATTTTATGAGACATAAAAATTAATTAATTTTTAAAATTTTTGAATATGTCAATAATTTATTAAATAAATAAATAAAAAGTTTAAAAATCTATTAAATTAATATAATTTAAAACTAATTATAAGAAATATTTTAAATTTTGTGTGCGTTTCAAAAATACATTTTTAATTTTAAAATTTGCAACGTTTTCTTTCGTTTTTTTTTTTTTTAGGGTTTCAAAAATTTCTAGTTTAAATCATTTAAAATTTTAAGCTAAAATTGAAATTTAAACGATTTACGAGTCTAGATCGTTTAGAATTTTAGATCAAAACTGAAATTTAAACGATTTAGGAGCGATTTCGAACGAAAATTTCAATTTCAATCCAAGAAGTCTTATTCTAAATTTGTAATTTGTGTAGTAAAAGTTGAGAATCTAATATTTGATGAACATTGTTGAGCAGATTACGCTTACTTGATTGACGACCAGTTGGCGAATTTGGGCGATGAGGCTTTGAGCATTCTTCACAAACTCCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAGCATATTCAGTAATGCTTCTAACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACTCTCGATCTACTCTGCGGCTGCGCCGACGGCAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTATTGTGTCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAAAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGCCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTCTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAG

mRNA sequence

ATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTTTGCCCCATCTCTCTCCAAATCATGAAAGACCCCGTCACGGTCGCTTCCGGCATAACCTATGACCGCAAAAGCATCGAGAAATGGTTGTTTTCAGACGGACACAAGACATGCCCCGTCACACAGGTTGTCTTATCGAGCTTCGACATCACTCCCAACATTACTCTAAGACGAGTCATTCAAGCTTGGTGTACTGTCAATGCTTGCAAGGGCATCGAGAGGATTCCGACACCTAAGCCACCGGTCGATTGTAAACAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTCCGTTCTCGCAACAGAATTGTCTCCGACGACTCCGATCTATTGCAGCGGCGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCCGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTTAGAAGATTACGCTTACTTGATTGACGACCAGTTGGCGAATTTGGGCGATGAGGCTTTGAGCATTCTTCACAAACTCCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAGCATATTCAGTAATGCTTCTAACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACTCTCGATCTACTCTGCGGCTGCGCCGACGGCAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTATTGTGTCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAAAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGCCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTCTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAG

Coding sequence (CDS)

ATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTTTGCCCCATCTCTCTCCAAATCATGAAAGACCCCGTCACGGTCGCTTCCGGCATAACCTATGACCGCAAAAGCATCGAGAAATGGTTGTTTTCAGACGGACACAAGACATGCCCCGTCACACAGGTTGTCTTATCGAGCTTCGACATCACTCCCAACATTACTCTAAGACGAGTCATTCAAGCTTGGTGTACTGTCAATGCTTGCAAGGGCATCGAGAGGATTCCGACACCTAAGCCACCGGTCGATTGTAAACAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTCCGTTCTCGCAACAGAATTGTCTCCGACGACTCCGATCTATTGCAGCGGCGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCCGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTTAGAAGATTACGCTTACTTGATTGACGACCAGTTGGCGAATTTGGGCGATGAGGCTTTGAGCATTCTTCACAAACTCCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAGCATATTCAGTAATGCTTCTAACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACTCTCGATCTACTCTGCGGCTGCGCCGACGGCAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTATTGTGTCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAAAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGCCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTCTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAG

Protein sequence

MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH
BLAST of Carg19550 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796.2 bits (2055), Expect = 5.4e-227
Identity = 413/423 (97.64%), Postives = 417/423 (98.58%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDR+SIEKWLFSD HKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPN TLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSI 180
           RLRSIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQ ANLGDEALSI
Sbjct: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180

Query: 181 LHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           L+KLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPV+IDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of Carg19550 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 792.7 bits (2046), Expect = 6.0e-226
Identity = 408/423 (96.45%), Postives = 418/423 (98.82%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD HKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPN T+RRVIQAWCT+NACKGIERIPTPKPP+DCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSI 180
           RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQ ANLGDEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           L+KLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PVLIDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of Carg19550 vs. NCBI nr
Match: XP_022950790.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 791.6 bits (2043), Expect = 1.3e-225
Identity = 411/423 (97.16%), Postives = 418/423 (98.82%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD HKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           +FDITPN TLRRVIQAWCTVNACKG+ERIPTPKPPVDCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSI 180
           RL+SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLG EALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           L+KLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 424
           FPH
Sbjct: 421 FPH 423

BLAST of Carg19550 vs. NCBI nr
Match: XP_022925148.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 681.0 bits (1756), Expect = 2.5e-192
Identity = 355/416 (85.34%), Postives = 379/416 (91.11%), Query Frame = 0

Query: 7   ELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFDITP 66
           E+DPPPFFLCPISLQIMKDPVTVASGITYDR++IE+WLFSD H TCPVTQVVLS FDITP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 67  NITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIA 126
           N TL R+IQAWCTVN    +ERIPTPKPPVD KQVV+ILAD KLSPFSQQNCLRRL SIA
Sbjct: 63  NNTLSRLIQAWCTVN--NRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 122

Query: 127 AASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILHKLQI 186
           +A+E NKRCMEAAGAVEFL G+VCNS  +E S  D+A+  DD LANLGDEALSIL+KLQI
Sbjct: 123 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAF--DDPLANLGDEALSILYKLQI 182

Query: 187 SESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFV 246
           SESGLKFLLANNGGIFV+TLTK LQNGTYGSRAY+ MLL SMFEVAD+IQI SLN +FFV
Sbjct: 183 SESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFV 242

Query: 247 EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCE 306
           EIVQILKDQISKQASKSALKLLIGLCSS RNRV AV+AGAVPVLIDLLLDLDSSEKR CE
Sbjct: 243 EIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCE 302

Query: 307 MILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESV 366
           MILV LDLLCGCADGRAE+LNHAAGIA+VSKKILRVS+LGSEKAVGILCSIARFSAS+SV
Sbjct: 303 MILVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSV 362

Query: 367 VQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 423
           VQEM RLGVV KLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSC PSSLR S+P
Sbjct: 363 VQEMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

BLAST of Carg19550 vs. NCBI nr
Match: XP_008441961.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo])

HSP 1 Score: 672.9 bits (1735), Expect = 6.9e-190
Identity = 353/427 (82.67%), Postives = 388/427 (90.87%), Query Frame = 0

Query: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQV 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDR+SIEKWLFSD HKTCPV+ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSSFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQN 120
           VLS FD+TPN TLRRVIQAWCTVNA KG+ERIPTPKPPVD +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGDE 180
           CL RLRSIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN  DE
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           AL ILHKLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIA+VSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 423
           +LRS++P
Sbjct: 421 TLRSAYP 425

BLAST of Carg19550 vs. TAIR10
Match: AT3G52450.1 (plant U-box 22)

HSP 1 Score: 425.2 bits (1092), Expect = 4.6e-119
Identity = 231/435 (53.10%), Postives = 307/435 (70.57%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDR+SIEKWLFS    +CPVT+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPN TLRR+IQ+WCT+NA  GIERIPTPKPP+   ++ +++ ++  S  +Q  CL+RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNST---NVEFSLEDYAYLID-----------DQ 183
            I + + +NKRC+EAA   EFLA IV NS    N   S    + L D           D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 LANLGDEALSILHKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRAYSVMLLTSM 243
             +L DEALS+L+ L  SE+ LK LL N  G   V TLTKI+Q G Y SRAY+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLL-DLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC CA+GRAE LNH A IA+VSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQV+  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVS-CGNKTKEKAKELLKLHARVW 420

BLAST of Carg19550 vs. TAIR10
Match: AT2G35930.1 (plant U-box 23)

HSP 1 Score: 414.8 bits (1065), Expect = 6.2e-116
Identity = 216/421 (51.31%), Postives = 303/421 (71.97%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR SIEKWLF+    +CPVT+  ++  D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPN TLRR+IQ+WCT+NA  G+ERIPTP+PP+   ++ +++ D+  S  +Q  CL+RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILHK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L DEAL++L+ 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC CA+GRAE LNH A IA+V KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQV+  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQVS-CGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

BLAST of Carg19550 vs. TAIR10
Match: AT3G11840.1 (plant U-box 24)

HSP 1 Score: 229.9 bits (585), Expect = 2.8e-60
Identity = 163/456 (35.75%), Postives = 243/456 (53.29%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I KWL  +   +CPVT+  L 
Sbjct: 15  MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 74

Query: 61  -SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK---LSPFSQQ 120
              D+TPN  LRR+IQ WC  N  +G+ RI TP+ P     VV  + + K        ++
Sbjct: 75  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 134

Query: 121 NCLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDE 180
             L++L  + A   +N+R M   G  + L   V   T+ +   ED    I        DE
Sbjct: 135 ETLQKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSED---EDGRRRIKGL-----DE 194

Query: 181 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           +L +LH + I  +  K +L  N  + + +LT +L    + S+AY+++LL ++ E      
Sbjct: 195 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 254

Query: 241 ILSLNTDFFVEIVQILKDQIS-----------------------------------KQAS 300
           +  LN + F  I+  LKD ++                                   KQA 
Sbjct: 255 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 314

Query: 301 KSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADG 360
            +AL +L+   S SRNR   V  GAV  LI+L +   + EKR  E++L  L  LC CA+G
Sbjct: 315 TAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANG 374

Query: 361 RAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCF 418
           RAE+L H  GIA+V+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC 
Sbjct: 375 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 434

BLAST of Carg19550 vs. TAIR10
Match: AT3G19380.1 (plant U-box 25)

HSP 1 Score: 195.3 bits (495), Expect = 7.7e-50
Identity = 149/424 (35.14%), Postives = 225/424 (53.07%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCPVT+  LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PN TLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEA 183
            LRRLR  A  S+ N+  + A  A E L  I+ + T                 + L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR---AYSVMLLTSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R   A  + ++++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q +    +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

BLAST of Carg19550 vs. TAIR10
Match: AT1G49780.1 (plant U-box 26)

HSP 1 Score: 188.7 bits (478), Expect = 7.2e-48
Identity = 147/424 (34.67%), Postives = 225/424 (53.07%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ + G+ TCPVT+V LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PN TLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLAN-LGDE 183
            +RRLR +A  SE N+  +    A E L  I+            +A +    L++ L  E
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL------------FADIETTSLSSELVSE 187

Query: 184 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 243
           +L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  + 
Sbjct: 188 SLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 247

Query: 244 ---ILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID 303
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID
Sbjct: 248 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 307

Query: 304 -LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAV 363
            L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSS 416
           G L  +A  +A E    E    G+VT+L  L+Q +    +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

BLAST of Carg19550 vs. Swiss-Prot
Match: sp|Q9SVC6|PUB22_ARATH (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 8.2e-118
Identity = 231/435 (53.10%), Postives = 307/435 (70.57%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDR+SIEKWLFS    +CPVT+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPN TLRR+IQ+WCT+NA  GIERIPTPKPP+   ++ +++ ++  S  +Q  CL+RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNST---NVEFSLEDYAYLID-----------DQ 183
            I + + +NKRC+EAA   EFLA IV NS    N   S    + L D           D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 LANLGDEALSILHKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRAYSVMLLTSM 243
             +L DEALS+L+ L  SE+ LK LL N  G   V TLTKI+Q G Y SRAY+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VLIDLLL-DLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC CA+GRAE LNH A IA+VSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQV+  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVS-CGNKTKEKAKELLKLHARVW 420

BLAST of Carg19550 vs. Swiss-Prot
Match: sp|Q84TG3|PUB23_ARATH (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.1e-114
Identity = 216/421 (51.31%), Postives = 303/421 (71.97%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR SIEKWLF+    +CPVT+  ++  D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPN TLRR+IQ+WCT+NA  G+ERIPTP+PP+   ++ +++ D+  S  +Q  CL+RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILHK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L DEAL++L+ 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC CA+GRAE LNH A IA+V KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQV+  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQVS-CGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

BLAST of Carg19550 vs. Swiss-Prot
Match: sp|Q9SF15|PUB24_ARATH (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 5.1e-59
Identity = 163/456 (35.75%), Postives = 243/456 (53.29%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I KWL  +   +CPVT+  L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 61  -SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK---LSPFSQQ 120
              D+TPN  LRR+IQ WC  N  +G+ RI TP+ P     VV  + + K        ++
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 121 NCLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDE 180
             L++L  + A   +N+R M   G  + L   V   T+ +   ED    I        DE
Sbjct: 121 ETLQKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSED---EDGRRRIKGL-----DE 180

Query: 181 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           +L +LH + I  +  K +L  N  + + +LT +L    + S+AY+++LL ++ E      
Sbjct: 181 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 240

Query: 241 ILSLNTDFFVEIVQILKDQIS-----------------------------------KQAS 300
           +  LN + F  I+  LKD ++                                   KQA 
Sbjct: 241 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 300

Query: 301 KSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADG 360
            +AL +L+   S SRNR   V  GAV  LI+L +   + EKR  E++L  L  LC CA+G
Sbjct: 301 TAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANG 360

Query: 361 RAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCF 418
           RAE+L H  GIA+V+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC 
Sbjct: 361 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 420

BLAST of Carg19550 vs. Swiss-Prot
Match: sp|Q9LT79|PUB25_ARATH (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.4e-48
Identity = 149/424 (35.14%), Postives = 225/424 (53.07%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCPVT+  LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PN TLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEA 183
            LRRLR  A  S+ N+  + A  A E L  I+ + T                 + L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 184 LSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR---AYSVMLLTSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R   A  + ++++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q +    +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

BLAST of Carg19550 vs. Swiss-Prot
Match: sp|Q9FXA4|PUB26_ARATH (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.3e-46
Identity = 147/424 (34.67%), Postives = 225/424 (53.07%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ + G+ TCPVT+V LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PN TLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLAN-LGDE 183
            +RRLR +A  SE N+  +    A E L  I+            +A +    L++ L  E
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRIL------------FADIETTSLSSELVSE 187

Query: 184 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 243
           +L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  + 
Sbjct: 188 SLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 247

Query: 244 ---ILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLID 303
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID
Sbjct: 248 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 307

Query: 304 -LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAV 363
            L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILCSIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSS 416
           G L  +A  +A E    E    G+VT+L  L+Q +    +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

BLAST of Carg19550 vs. TrEMBL
Match: tr|A0A1S3B4K2|A0A1S3B4K2_CUCME (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 4.6e-190
Identity = 353/427 (82.67%), Postives = 388/427 (90.87%), Query Frame = 0

Query: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQV 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDR+SIEKWLFSD HKTCPV+ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSSFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQN 120
           VLS FD+TPN TLRRVIQAWCTVNA KG+ERIPTPKPPVD +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGDE 180
           CL RLRSIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN  DE
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           AL ILHKLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIA+VSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 423
           +LRS++P
Sbjct: 421 TLRSAYP 425

BLAST of Carg19550 vs. TrEMBL
Match: tr|A0A0A0L1F5|A0A0A0L1F5_CUCSA (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 4.2e-183
Identity = 341/425 (80.24%), Postives = 378/425 (88.94%), Query Frame = 0

Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDR+SIEKWL S  H TCPV+ +VLS
Sbjct: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60

Query: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
            F ITPN TLRRVIQAWCT+NA KG+ERIPTPKPPVD +QVVRILA A LSPFSQ+N L 
Sbjct: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120

Query: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCN--STNVEFSLEDYAYLIDDQLANLGDEAL 180
           RLRSIA A+ESNKRCME+AG VEFLAG+VCN  +TN+E+ LEDYA+  DD  AN  DEAL
Sbjct: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 180

Query: 181 SILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240
            ILHKLQ+SES LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMF+VADQIQI 
Sbjct: 181 LILHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQIQ 240

Query: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSI 360
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIA+VSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 360

Query: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420
           A+FS S+SVVQEM+R+GVVTKLCF+LQV GAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSSFP 423
           RS++P
Sbjct: 421 RSAYP 423

BLAST of Carg19550 vs. TrEMBL
Match: tr|W9SK34|W9SK34_9ROSA (RING-type E3 ubiquitin transferase OS=Morus notabilis OX=981085 GN=L484_016918 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 5.3e-138
Identity = 263/427 (61.59%), Postives = 337/427 (78.92%), Query Frame = 0

Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFD 63
           +D E+D PPFFLCPISL+IMKDPVTV +GITYDR+SIEKWLFS  + TCPVT+ VLS  D
Sbjct: 1   MDTEIDVPPFFLCPISLEIMKDPVTVLTGITYDRESIEKWLFSGKNDTCPVTKQVLSDSD 60

Query: 64  ITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPN TLRR+IQAWCT+NA  GIERIPTPKPP+   Q+ ++L+DAK SP SQ  CLRRLR
Sbjct: 61  LTPNHTLRRLIQAWCTMNAALGIERIPTPKPPISKSQIAKLLSDAK-SPQSQIKCLRRLR 120

Query: 124 SIAAASESNKRCMEAAGAVEFLAGIVCNSTN-------VEFSLEDYAYLIDDQLANLGDE 183
           SIAA SE+NKRC+E+AGAVEFL  ++ N  N       +  S  D ++   D   +  DE
Sbjct: 121 SIAAESETNKRCLESAGAVEFLVSLIANDNNENAVSSPLSSSENDESF---DFARSASDE 180

Query: 184 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 243
           AL+ILH L +SE+ LK L+  NG  FV +LT++++ G+Y SRAYS+MLL SMF+VAD ++
Sbjct: 181 ALTILHNLHLSETVLKKLIDRNGE-FVDSLTRVMRQGSYDSRAYSIMLLKSMFDVADPMR 240

Query: 244 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 303
           ++SL T+F+ E+VQIL+DQIS QASK+AL+LL+ LC   RNR+KAV+AGAV VLI+LL  
Sbjct: 241 MISLRTEFYAELVQILRDQISPQASKAALQLLVQLCPWGRNRIKAVEAGAVAVLIELL-- 300

Query: 304 LDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCS 363
           LDS E+R CEMIL  LDL+CGCA+GRAE++ HAAG+A+VSK++LRVS +GSEKA+ IL S
Sbjct: 301 LDSVERRPCEMILTGLDLMCGCAEGRAELIRHAAGLAVVSKRVLRVSHIGSEKAMRILFS 360

Query: 364 IARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSS 423
           +ARFSA+ +V+ EM++LGVV KLC +LQV   GGK KEKA+EIL+LH RTWRNS C+P  
Sbjct: 361 VARFSATPAVLAEMLQLGVVAKLCLVLQV-DCGGKTKEKAREILRLHARTWRNSPCVPMR 419

BLAST of Carg19550 vs. TrEMBL
Match: tr|A0A2C9VD36|A0A2C9VD36_MANES (RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_08G034100 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.1e-134
Identity = 259/419 (61.81%), Postives = 326/419 (77.80%), Query Frame = 0

Query: 6   QELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFDIT 65
           +E+D PPFFLCPISLQIMKDPVTV +GITYDR+SIEKWLFS  + TCPVT+ V+S  D+T
Sbjct: 2   EEIDVPPFFLCPISLQIMKDPVTVPTGITYDRESIEKWLFSGKNDTCPVTKQVISDCDLT 61

Query: 66  PNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSI 125
           PN+TL R+IQ+WCT+NA  GIERIPTPKPP+   Q+ +I+ DA  SP  Q  CL+RLRSI
Sbjct: 62  PNLTLMRLIQSWCTLNASHGIERIPTPKPPISKAQIAKIIKDANKSPQQQITCLKRLRSI 121

Query: 126 AAASESNKRCMEAAGAVEFLAGIV--CNSTNVEFSLEDYAYLIDDQLANLGDEALSILHK 185
           A  +++NKRCMEAAGAVEFLA IV   NS + E S  D     + ++    DEALSIL+ 
Sbjct: 122 ANLNDANKRCMEAAGAVEFLASIVNGFNSLSFEQSTSDGD---ESEVTRPSDEALSILYA 181

Query: 186 LQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTD 245
           LQ+SESGLK L+ NNG I + +LT ILQNG Y SRAY+V+LL S+FEVAD +Q+++L  +
Sbjct: 182 LQLSESGLKTLIGNNGEI-IQSLTNILQNGNYESRAYAVLLLKSVFEVADPMQLINLRIE 241

Query: 246 FFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKR 305
            F EIVQ+L DQIS QA K+AL+LLI LC   RNR++AV+A AV VLIDLL  LDS +KR
Sbjct: 242 LFAEIVQVLSDQISHQAHKAALQLLISLCPWGRNRIRAVEANAVSVLIDLL--LDSRDKR 301

Query: 306 SCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSAS 365
           +CEM L  L+LLC CA+GRAE+L H AG+AIVSKKILRVS + SEKAV IL S+++FSA+
Sbjct: 302 ACEMALNVLELLCQCAEGRAELLRHGAGLAIVSKKILRVSQVASEKAVRILLSVSKFSAT 361

Query: 366 ESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 423
            SV+QEM+++GVV KLC +LQV   G K K+KAKE+LKLH R W+NS+C+P++L   +P
Sbjct: 362 PSVLQEMLQIGVVAKLCLVLQV-DCGSKTKDKAKEVLKLHARAWKNSACIPANLLFFYP 413

BLAST of Carg19550 vs. TrEMBL
Match: tr|B9SDI7|B9SDI7_RICCO (RING-type E3 ubiquitin transferase OS=Ricinus communis OX=3988 GN=RCOM_0419110 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 3.3e-132
Identity = 251/417 (60.19%), Postives = 326/417 (78.18%), Query Frame = 0

Query: 6   QELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLSSFDIT 65
           +++D P FFLCPISLQ+M DPVTV +GITYDR+SIEKWLF+  + TCPVT+ ++S  D+T
Sbjct: 2   EDIDVPSFFLCPISLQLMTDPVTVPTGITYDRQSIEKWLFAGKNHTCPVTKQLISDCDLT 61

Query: 66  PNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSI 125
           PN+TL R+IQ+WCT+NA  G+ERIPTPKPP+  +Q+ ++L DAK SP  Q  CLR+LRSI
Sbjct: 62  PNLTLMRLIQSWCTLNASHGVERIPTPKPPISKQQIAKLLKDAK-SPQQQLKCLRKLRSI 121

Query: 126 AAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILHKLQ 185
           A  +++NKRCMEAAGAVEFLA IV N T++ F  E        ++A L DEALSIL+ LQ
Sbjct: 122 ANVNDTNKRCMEAAGAVEFLASIVNNFTSLSFE-ESSLDGAGIEIARLSDEALSILYSLQ 181

Query: 186 ISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFF 245
           +SESGL+ L+  NG + + +LT+++QNG Y SRAY+V+LL S  EVAD +Q++SL  + F
Sbjct: 182 LSESGLRNLIGKNGEV-IQSLTRVMQNGNYESRAYAVLLLKSALEVADPMQLVSLRPELF 241

Query: 246 VEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSC 305
           VEIVQ+L DQIS QASK+AL+LLI LC   R+R+KA +A AV  LIDLL  LDSSEKR+C
Sbjct: 242 VEIVQVLSDQISHQASKAALQLLINLCPWGRSRLKATEAKAVSTLIDLL--LDSSEKRTC 301

Query: 306 EMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASES 365
           EM L  LDLLC CA+GRAE+L H AG+A+VSKKILRVS + SEKAV IL SI++FSA+ +
Sbjct: 302 EMALAVLDLLCQCAEGRAELLKHGAGLAVVSKKILRVSQVASEKAVRILLSISKFSATST 361

Query: 366 VVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 423
           V+ EM+++GVV KLC +LQV   G K K+KA+E+LKLH R W+NS C+P++L SS+P
Sbjct: 362 VLHEMLQIGVVAKLCLVLQV-DCGSKGKDKAREVLKLHARAWKNSPCIPANLLSSYP 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023543358.15.4e-22797.64E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo][more]
XP_022978192.16.0e-22696.45E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
XP_022950790.11.3e-22597.16E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata][more]
XP_022925148.12.5e-19285.34E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata][more]
XP_008441961.16.9e-19082.67PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G52450.14.6e-11953.10plant U-box 22[more]
AT2G35930.16.2e-11651.31plant U-box 23[more]
AT3G11840.12.8e-6035.75plant U-box 24[more]
AT3G19380.17.7e-5035.14plant U-box 25[more]
AT1G49780.17.2e-4834.67plant U-box 26[more]
Match NameE-valueIdentityDescription
sp|Q9SVC6|PUB22_ARATH8.2e-11853.10E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
sp|Q84TG3|PUB23_ARATH1.1e-11451.31E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
sp|Q9SF15|PUB24_ARATH5.1e-5935.75E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
sp|Q9LT79|PUB25_ARATH1.4e-4835.14U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
sp|Q9FXA4|PUB26_ARATH1.3e-4634.67U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3B4K2|A0A1S3B4K2_CUCME4.6e-19082.67RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 ... [more]
tr|A0A0A0L1F5|A0A0A0L1F5_CUCSA4.2e-18380.24RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE... [more]
tr|W9SK34|W9SK34_9ROSA5.3e-13861.59RING-type E3 ubiquitin transferase OS=Morus notabilis OX=981085 GN=L484_016918 P... [more]
tr|A0A2C9VD36|A0A2C9VD36_MANES2.1e-13461.81RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_08G0341... [more]
tr|B9SDI7|B9SDI7_RICCO3.3e-13260.19RING-type E3 ubiquitin transferase OS=Ricinus communis OX=3988 GN=RCOM_0419110 P... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0004842ubiquitin-protein transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: INTERPRO
TermDefinition
IPR016024ARM-type_fold
IPR013083Znf_RING/FYVE/PHD
IPR011989ARM-like
IPR003613Ubox_domain
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0005488 binding
molecular_function GO:0004842 ubiquitin-protein transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19550-RACarg19550-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 13..77
e-value: 2.3E-33
score: 126.9
IPR003613U box domainPFAMPF04564U-boxcoord: 11..79
e-value: 1.2E-20
score: 73.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 9..84
score: 41.293
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 87..403
e-value: 4.5E-15
score: 57.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 1..86
e-value: 4.1E-27
score: 96.1
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 7..422
NoneNo IPR availablePANTHERPTHR22849:SF54SUBFAMILY NOT NAMEDcoord: 7..422
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 14..56
e-value: 1.47388E-22
score: 88.8298
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 7..85
IPR016024Armadillo-type foldSUPERFAMILYSSF48371ARM repeatcoord: 102..385