MELO3C008784.2 (gene) Melon (DHL92) v3.6.1

NameMELO3C008784.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr08 : 26709099 .. 26710847 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTTCATTTCAAAACCTAACGTTTTCACAACTTCCCAACAACTCTTTTCTCTCATCTGATCTCCCTCTAACAACCCTTTCTCTTCCTCTAAAGATCATTCTATTATTTCTCTAGCTAGTTGAGCCATTCATGGCAGAATTAGATGATCAAGATCAAGTAGTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTGCAAATCATGAAAGATCCCGTTACCATCGCCTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCCGACAAACACAAGACATGCCCCGTCTCGCACATAGTTTTGTCAGACTTTGACGTCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGTCAATGCTTCTAAAGGTGTAGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTTGTTCGGATTCTTGCCGATGCTAAGTTATCTCCATTCTCTCAAAAGAATTGCCTTTGTCGCCTTCGATCTATTGCTGCGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATTCTACCACCAACATGGAGTATGGCTTAGAAGGTAATATTCAAAACTCAAAACAAATTTAAAGTTTCTTATTTTGCATAGTTTGAACACTACGAACCCCTCATTGCCAATAATATATGTGTAGTTATAATGTATAAAATAAGCTTTGAGAATTTAATAATATTGGTTATTTATGCATATTTGATGATGCAGATTACGCGTTTGACGACATGTCGGCAAATTCAGCCGATGAGGCTTTGATTATTCTTCATAAACTCCAAATCTCCGAATCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTATTCCTCTCGATCATATTCAGTAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATCCAAAATCTCACCGCCGATTTCTTTATCGAAATCATCCAAATCCTCAAAGATCAAATCTCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCAGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTCGATCAGCTCTGTGGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCATGCGGCGGGGATTGCCGTGGTGTCGAAGAAGATTCTGAGAATTTCGACGGTTGGAAGCGAGAAAGCGGTTGGGATCTTGTGGTCTATAGCGAGATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGAGGATTGGTGTTGTGACGAAGCTGTGTTTTCTGCTTCAGGTGGTGGGCGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCGTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAATATTATTAGGGAGGGTTTGCTTTTCTTTATTTGTTTCTAAGTGCATATATATATATAGTTGAGTTGATTGAAATTGAATTTGTATATTTTAAAAATTTGTACATATGCTTTGTGTAAATAATTGGTTCATAATTCAAGTAGGATTAATGTAATCTGGATATAGTTAGCATTTGTATT

mRNA sequence

CTCTCTTCATTTCAAAACCTAACGTTTTCACAACTTCCCAACAACTCTTTTCTCTCATCTGATCTCCCTCTAACAACCCTTTCTCTTCCTCTAAAGATCATTCTATTATTTCTCTAGCTAGTTGAGCCATTCATGGCAGAATTAGATGATCAAGATCAAGTAGTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTGCAAATCATGAAAGATCCCGTTACCATCGCCTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCCGACAAACACAAGACATGCCCCGTCTCGCACATAGTTTTGTCAGACTTTGACGTCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGTCAATGCTTCTAAAGGTGTAGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTTGTTCGGATTCTTGCCGATGCTAAGTTATCTCCATTCTCTCAAAAGAATTGCCTTTGTCGCCTTCGATCTATTGCTGCGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATTCTACCACCAACATGGAGTATGGCTTAGAAGATTACGCGTTTGACGACATGTCGGCAAATTCAGCCGATGAGGCTTTGATTATTCTTCATAAACTCCAAATCTCCGAATCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTATTCCTCTCGATCATATTCAGTAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATCCAAAATCTCACCGCCGATTTCTTTATCGAAATCATCCAAATCCTCAAAGATCAAATCTCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCAGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTCGATCAGCTCTGTGGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCATGCGGCGGGGATTGCCGTGGTGTCGAAGAAGATTCTGAGAATTTCGACGGTTGGAAGCGAGAAAGCGGTTGGGATCTTGTGGTCTATAGCGAGATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGAGGATTGGTGTTGTGACGAAGCTGTGTTTTCTGCTTCAGGTGGTGGGCGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCGTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAATATTATTAGGGAGGGTTTGCTTTTCTTTATTTGTTTCTAAGTGCATATATATATATAGTTGAGTTGATTGAAATTGAATTTGTATATTTTAAAAATTTGTACATATGCTTTGTGTAAATAATTGGTTCATAATTCAAGTAGGATTAATGTAATCTGGATATAGTTAGCATTTGTATT

Coding sequence (CDS)

ATGGCAGAATTAGATGATCAAGATCAAGTAGTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTGCAAATCATGAAAGATCCCGTTACCATCGCCTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCCGACAAACACAAGACATGCCCCGTCTCGCACATAGTTTTGTCAGACTTTGACGTCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGTCAATGCTTCTAAAGGTGTAGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTTGTTCGGATTCTTGCCGATGCTAAGTTATCTCCATTCTCTCAAAAGAATTGCCTTTGTCGCCTTCGATCTATTGCTGCGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATTCTACCACCAACATGGAGTATGGCTTAGAAGATTACGCGTTTGACGACATGTCGGCAAATTCAGCCGATGAGGCTTTGATTATTCTTCATAAACTCCAAATCTCCGAATCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTATTCCTCTCGATCATATTCAGTAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATCCAAAATCTCACCGCCGATTTCTTTATCGAAATCATCCAAATCCTCAAAGATCAAATCTCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCAGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTCGATCAGCTCTGTGGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCATGCGGCGGGGATTGCCGTGGTGTCGAAGAAGATTCTGAGAATTTCGACGGTTGGAAGCGAGAAAGCGGTTGGGATCTTGTGGTCTATAGCGAGATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGAGGATTGGTGTTGTGACGAAGCTGTGTTTTCTGCTTCAGGTGGTGGGCGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCGTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAA

Protein sequence

MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYPQ
BLAST of MELO3C008784.2 vs. NCBI nr
Match: XP_008441961.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo])

HSP 1 Score: 819.3 bits (2115), Expect = 6.0e-234
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180
           CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240
           IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300
           NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360
           SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420
           ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSAYPQ 427
           RSAYPQ
Sbjct: 421 RSAYPQ 426

BLAST of MELO3C008784.2 vs. NCBI nr
Match: XP_011653475.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis sativus] >KGN53961.1 hypothetical protein Csa_4G194780 [Cucumis sativus])

HSP 1 Score: 761.5 bits (1965), Expect = 1.5e-216
Identity = 397/427 (92.97%), Postives = 410/427 (96.02%), Query Frame = 0

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAEL   DQV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAEL---DQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVC-NSTTNMEYGLEDYAFDDMSANSADEA 180
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVC NSTTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 180

Query: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240
           L+ILHKLQ+SESSLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMF+VADQIQI
Sbjct: 181 LLILHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQI 240

Query: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 360

Query: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 421 LRSAYPQ 427
           LRSAYPQ
Sbjct: 421 LRSAYPQ 424

BLAST of MELO3C008784.2 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 674.5 bits (1739), Expect = 2.4e-190
Identity = 352/427 (82.44%), Postives = 390/427 (91.33%), Query Frame = 0

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIE+WLFSD+HKTCPV+ +
Sbjct: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQV 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLS FD+TPNHT+RRVIQAWCT+NA KG+ERIPTPKPP+D +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180
           CL RLRSIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN  DE
Sbjct: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQFANLGDE 180

Query: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240

Query: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300
           I +L  DFFIEI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGA+PVLIDLLLD
Sbjct: 241 ILSLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLD 300

Query: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360
           LDSSE KR CEMILV LD LC CADGRAE+LNHAAGIAVVSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSE-KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILC 360

Query: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 TLRSAYP 426
           +LRS++P
Sbjct: 421 SLRSSFP 422

BLAST of MELO3C008784.2 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674.1 bits (1738), Expect = 3.1e-190
Identity = 353/427 (82.67%), Postives = 389/427 (91.10%), Query Frame = 0

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIEKWLFSD+HKTCPV+ +
Sbjct: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQV 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLS FD+TPNHTLRRVIQAWCTVNA KG+ERIPTPKPPVD +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSSFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180
           CL RLRSIAA +ESNKRCME+AG VEFLAG+VCNS TN+E+  EDYA+  DD  AN  DE
Sbjct: 121 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNS-TNVEFSSEDYAYLIDDQFANLGDE 180

Query: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240

Query: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPV+IDLLLD
Sbjct: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLD 300

Query: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIAVVSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILC 360

Query: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 TLRSAYP 426
           +LRS++P
Sbjct: 421 SLRSSFP 422

BLAST of MELO3C008784.2 vs. NCBI nr
Match: XP_022950790.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 667.9 bits (1722), Expect = 2.2e-188
Identity = 350/427 (81.97%), Postives = 389/427 (91.10%), Query Frame = 0

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAEL   DQ +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIE+WLFSD+HKTCP++ +
Sbjct: 1   MAEL---DQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQV 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLS+FD+TPNHTLRRVIQAWCTVNA KGVERIPTPKPPVD +QVVRILADAKLS FSQ+N
Sbjct: 61  VLSNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180
           CL RL+SIAAA+ESNKRCME+AG VEFLAG+VCNS TN+E+ LEDYA+  DD  AN   E
Sbjct: 121 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGFE 180

Query: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 240

Query: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300

Query: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIA+VSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILC 360

Query: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 TLRSAYP 426
           +LRS++P
Sbjct: 421 SLRSSFP 422

BLAST of MELO3C008784.2 vs. TAIR10
Match: AT3G52450.1 (plant U-box 22)

HSP 1 Score: 413.7 bits (1062), Expect = 1.4e-115
Identity = 219/434 (50.46%), Postives = 302/434 (69.59%), Query Frame = 0

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           DQ ++ P +FLCPISL IMKDPV +++GITYDRESIEKWLFS K  +CPV+  V+++ D+
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDL 61

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS G+ERIPTPKPP+ + ++ +++ ++  S  +Q  CL RLR 
Sbjct: 62  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 121

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCN------------STTNMEYGLEDYAFD---DMS 187
           I + N +NKRC+E+A V EFLA +V N            S++N+    +    +   D S
Sbjct: 122 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 181

Query: 188 ANSADEALIILHKLQISESSLKFLLSNNGGI-FISTLTKILQNRSYSSRSYSVMLLNSMF 247
            +  DEAL +L+ L  SE++LK LL+N  G   + TLTKI+Q   Y SR+Y+ +LL  + 
Sbjct: 182 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 241

Query: 248 EVADQIQIQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPV 307
           EVAD +QI  L  + F E+IQIL DQIS +A++SA+++L+      RNR KAV+ G + +
Sbjct: 242 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 301

Query: 308 LIDLLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSE 367
           +I+LL+D   S  +R  EM +V+LD LC CA+GRAE LNH A IAVVSKKILR+S + SE
Sbjct: 302 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 361

Query: 368 KAVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWR 426
           +AV +L S+ RF  + S++QEM+++GVV KLC +LQ V  G K KEKAKE+LKLH R WR
Sbjct: 362 RAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWR 421

BLAST of MELO3C008784.2 vs. TAIR10
Match: AT2G35930.1 (plant U-box 23)

HSP 1 Score: 403.7 bits (1036), Expect = 1.4e-112
Identity = 208/419 (49.64%), Postives = 295/419 (70.41%), Query Frame = 0

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           D+ ++ PP+FLCPISL+IMKDPV +++GITYDR+SIEKWLF+ K  +CPV+   ++D D+
Sbjct: 7   DEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADL 66

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS GVERIPTP+PP+ + ++ +++ D+  S  +Q  CL RLR 
Sbjct: 67  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 126

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQ 187
           I + N +NKRC+E+AGV EFLA +V N + N               +  DEAL +L+ L+
Sbjct: 127 IVSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLE 186

Query: 188 ISESSLKFLLSN-NGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADF 247
            SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q   L  + 
Sbjct: 187 TSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV 246

Query: 248 FIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKR 307
           F E++QIL D+IS++A+K+A+ +L+ +    RNR KAV+AG + V+I+LL+D   +  +R
Sbjct: 247 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 306

Query: 308 LCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGS 367
             EM +V+LD LC CA+GRAE LNH A IAVV KKILR+S   S++AV +L S+ RF  +
Sbjct: 307 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 366

Query: 368 QSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
            +++ EM+++GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP  +  AYP
Sbjct: 367 PALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of MELO3C008784.2 vs. TAIR10
Match: AT3G11840.1 (plant U-box 24)

HSP 1 Score: 253.1 bits (645), Expect = 3.1e-67
Identity = 166/457 (36.32%), Postives = 251/457 (54.92%), Query Frame = 0

Query: 3   ELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVL 62
           ++D +++ ++ P YF+CPISL+IMKDPVT  SGITYDR++I KWL  +K  +CPV+   L
Sbjct: 14  QMDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPL 73

Query: 63  S-DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK---LSPFSQ 122
             D D+TPNH LRR+IQ WC  N ++GV RI TP+ P  +  VV  + + K        +
Sbjct: 74  PLDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGR 133

Query: 123 KNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADE 182
           +  L +L  + A + +N+R M   GV + L   V   T+  E G              DE
Sbjct: 134 EETLQKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRR-------RIKGLDE 193

Query: 183 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 242
           +L +LH + I  +  K +L  N  + + +LT +L    + S++Y+++LL ++ E      
Sbjct: 194 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 253

Query: 243 IQNLTADFFIEIIQILKDQIS-----------------------------------KQAS 302
           ++ L  + F  II  LKD ++                                   KQA 
Sbjct: 254 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 313

Query: 303 KSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDQLCGCAD 362
            +AL +L+  SS  RNR   V  GAV  LI+  L++  +  KR+ E++L +L +LC CA+
Sbjct: 314 TAALMILLETSSWSRNRSLLVDLGAVSELIE--LEISYTGEKRITELMLGVLSRLCCCAN 373

Query: 363 GRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVVQEMVRIGVVTKLC 421
           GRAE+L H  GIAVV+K++LR+S    ++A+ IL ++++FS    VV+EMV +G V KLC
Sbjct: 374 GRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLC 433

BLAST of MELO3C008784.2 vs. TAIR10
Match: AT3G19380.1 (plant U-box 25)

HSP 1 Score: 183.3 bits (464), Expect = 3.0e-46
Identity = 137/418 (32.78%), Postives = 217/418 (51.91%), Query Frame = 0

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           ++  D  +  P +F CPISL++M+DPVT+ +G TYDR SIE W+    + TCPV+   LS
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   L RLR  A  ++ N+  + +    E L  ++ + TT+ E                 
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSEL--------------VS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L I+E +    +S++ G  +  LT++L + S  +R  +  L+  +       
Sbjct: 185 ESLALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 244

Query: 244 QIQNLTAD---FFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++   ++    F  ++ +L++ I S++A K  +K L A+ S    R  A+ AGA  +LI
Sbjct: 245 DLKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTW 411
           A G L  +A  +  +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 365 AAGAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C008784.2 vs. TAIR10
Match: AT1G49780.1 (plant U-box 26)

HSP 1 Score: 179.1 bits (453), Expect = 5.7e-45
Identity = 137/426 (32.16%), Postives = 219/426 (51.41%), Query Frame = 0

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           L+  D  +  P +F CPISL +M DPVTI++G TYDR SI+ W+ +  + TCPV+ + LS
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   + RLR +A  +E N+  +      E L  ++            D     +S+    
Sbjct: 125 RAAAIRRLRGLARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L ++E+  + + S+     +  +T++L + S   R  +  L+  +   A  +
Sbjct: 185 ESLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSM 244

Query: 244 QIQNLTA---DFFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++ + +     F  ++ +LK+ I S++A K  +K + A+    + R  A+ AGA  +LI
Sbjct: 245 DLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRN 419
           A G L  +A  +  +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W +
Sbjct: 365 AAGAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPD 410

BLAST of MELO3C008784.2 vs. Swiss-Prot
Match: sp|Q9SVC6|PUB22_ARATH (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.5e-114
Identity = 219/434 (50.46%), Postives = 302/434 (69.59%), Query Frame = 0

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           DQ ++ P +FLCPISL IMKDPV +++GITYDRESIEKWLFS K  +CPV+  V+++ D+
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDL 61

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS G+ERIPTPKPP+ + ++ +++ ++  S  +Q  CL RLR 
Sbjct: 62  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 121

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCN------------STTNMEYGLEDYAFD---DMS 187
           I + N +NKRC+E+A V EFLA +V N            S++N+    +    +   D S
Sbjct: 122 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 181

Query: 188 ANSADEALIILHKLQISESSLKFLLSNNGGI-FISTLTKILQNRSYSSRSYSVMLLNSMF 247
            +  DEAL +L+ L  SE++LK LL+N  G   + TLTKI+Q   Y SR+Y+ +LL  + 
Sbjct: 182 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 241

Query: 248 EVADQIQIQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPV 307
           EVAD +QI  L  + F E+IQIL DQIS +A++SA+++L+      RNR KAV+ G + +
Sbjct: 242 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 301

Query: 308 LIDLLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSE 367
           +I+LL+D   S  +R  EM +V+LD LC CA+GRAE LNH A IAVVSKKILR+S + SE
Sbjct: 302 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 361

Query: 368 KAVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWR 426
           +AV +L S+ RF  + S++QEM+++GVV KLC +LQ V  G K KEKAKE+LKLH R WR
Sbjct: 362 RAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWR 421

BLAST of MELO3C008784.2 vs. Swiss-Prot
Match: sp|Q84TG3|PUB23_ARATH (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.6e-111
Identity = 208/419 (49.64%), Postives = 295/419 (70.41%), Query Frame = 0

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           D+ ++ PP+FLCPISL+IMKDPV +++GITYDR+SIEKWLF+ K  +CPV+   ++D D+
Sbjct: 7   DEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADL 66

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS GVERIPTP+PP+ + ++ +++ D+  S  +Q  CL RLR 
Sbjct: 67  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 126

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQ 187
           I + N +NKRC+E+AGV EFLA +V N + N               +  DEAL +L+ L+
Sbjct: 127 IVSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLE 186

Query: 188 ISESSLKFLLSN-NGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADF 247
            SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q   L  + 
Sbjct: 187 TSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV 246

Query: 248 FIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKR 307
           F E++QIL D+IS++A+K+A+ +L+ +    RNR KAV+AG + V+I+LL+D   +  +R
Sbjct: 247 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 306

Query: 308 LCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGS 367
             EM +V+LD LC CA+GRAE LNH A IAVV KKILR+S   S++AV +L S+ RF  +
Sbjct: 307 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 366

Query: 368 QSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
            +++ EM+++GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP  +  AYP
Sbjct: 367 PALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of MELO3C008784.2 vs. Swiss-Prot
Match: sp|Q9SF15|PUB24_ARATH (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 9.6e-66
Identity = 166/456 (36.40%), Postives = 250/456 (54.82%), Query Frame = 0

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           +D +++ ++ P YF+CPISL+IMKDPVT  SGITYDR++I KWL  +K  +CPV+   L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 64  -DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK---LSPFSQK 123
            D D+TPNH LRR+IQ WC  N ++GV RI TP+ P  +  VV  + + K        ++
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 124 NCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEA 183
             L +L  + A + +N+R M   GV + L   V   T+  E G              DE+
Sbjct: 121 ETLQKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRR-------RIKGLDES 180

Query: 184 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 243
           L +LH + I  +  K +L  N  + + +LT +L    + S++Y+++LL ++ E      +
Sbjct: 181 LRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIV 240

Query: 244 QNLTADFFIEIIQILKDQIS-----------------------------------KQASK 303
           + L  + F  II  LKD ++                                   KQA  
Sbjct: 241 ERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVT 300

Query: 304 SALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDQLCGCADG 363
           +AL +L+  SS  RNR   V  GAV  LI+  L++  +  KR+ E++L +L +LC CA+G
Sbjct: 301 AALMILLETSSWSRNRSLLVDLGAVSELIE--LEISYTGEKRITELMLGVLSRLCCCANG 360

Query: 364 RAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVVQEMVRIGVVTKLCF 421
           RAE+L H  GIAVV+K++LR+S    ++A+ IL ++++FS    VV+EMV +G V KLC 
Sbjct: 361 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 420

BLAST of MELO3C008784.2 vs. Swiss-Prot
Match: sp|Q9LT79|PUB25_ARATH (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 5.5e-45
Identity = 137/418 (32.78%), Postives = 217/418 (51.91%), Query Frame = 0

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           ++  D  +  P +F CPISL++M+DPVT+ +G TYDR SIE W+    + TCPV+   LS
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   L RLR  A  ++ N+  + +    E L  ++ + TT+ E                 
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSEL--------------VS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L I+E +    +S++ G  +  LT++L + S  +R  +  L+  +       
Sbjct: 185 ESLALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 244

Query: 244 QIQNLTAD---FFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++   ++    F  ++ +L++ I S++A K  +K L A+ S    R  A+ AGA  +LI
Sbjct: 245 DLKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTW 411
           A G L  +A  +  +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 365 AAGAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C008784.2 vs. Swiss-Prot
Match: sp|Q9FXA4|PUB26_ARATH (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.0e-43
Identity = 137/426 (32.16%), Postives = 219/426 (51.41%), Query Frame = 0

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           L+  D  +  P +F CPISL +M DPVTI++G TYDR SI+ W+ +  + TCPV+ + LS
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   + RLR +A  +E N+  +      E L  ++            D     +S+    
Sbjct: 125 RAAAIRRLRGLARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L ++E+  + + S+     +  +T++L + S   R  +  L+  +   A  +
Sbjct: 185 ESLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSM 244

Query: 244 QIQNLTA---DFFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++ + +     F  ++ +LK+ I S++A K  +K + A+    + R  A+ AGA  +LI
Sbjct: 245 DLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRN 419
           A G L  +A  +  +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W +
Sbjct: 365 AAGAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPD 410

BLAST of MELO3C008784.2 vs. TrEMBL
Match: tr|A0A1S3B4K2|A0A1S3B4K2_CUCME (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 4.0e-234
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180
           CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240
           IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300
           NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360
           SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420
           ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSAYPQ 427
           RSAYPQ
Sbjct: 421 RSAYPQ 426

BLAST of MELO3C008784.2 vs. TrEMBL
Match: tr|A0A0A0L1F5|A0A0A0L1F5_CUCSA (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 9.9e-217
Identity = 397/427 (92.97%), Postives = 410/427 (96.02%), Query Frame = 0

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAEL   DQV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAEL---DQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVC-NSTTNMEYGLEDYAFDDMSANSADEA 180
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVC NSTTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 180

Query: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240
           L+ILHKLQ+SESSLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMF+VADQIQI
Sbjct: 181 LLILHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQI 240

Query: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 360

Query: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 421 LRSAYPQ 427
           LRSAYPQ
Sbjct: 421 LRSAYPQ 424

BLAST of MELO3C008784.2 vs. TrEMBL
Match: tr|W9SK34|W9SK34_9ROSA (RING-type E3 ubiquitin transferase OS=Morus notabilis OX=981085 GN=L484_016918 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 2.9e-131
Identity = 248/424 (58.49%), Postives = 331/424 (78.07%), Query Frame = 0

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           D  +D PP+FLCPISL+IMKDPVT+ +GITYDRESIEKWLFS K+ TCPV+  VLSD D+
Sbjct: 2   DTEIDVPPFFLCPISLEIMKDPVTVLTGITYDRESIEKWLFSGKNDTCPVTKQVLSDSDL 61

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQAWCT+NA+ G+ERIPTPKPP+ + Q+ ++L+DAK SP SQ  CL RLRS
Sbjct: 62  TPNHTLRRLIQAWCTMNAALGIERIPTPKPPISKSQIAKLLSDAK-SPQSQIKCLRRLRS 121

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCNS------TTNMEYGLEDYAFDDMSANSADEALI 187
           IAA +E+NKRC+ESAG VEFL  ++ N       ++ +     D +F D + +++DEAL 
Sbjct: 122 IAAESETNKRCLESAGAVEFLVSLIANDNNENAVSSPLSSSENDESF-DFARSASDEALT 181

Query: 188 ILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQN 247
           ILH L +SE+ LK L+  NG  F+ +LT++++  SY SR+YS+MLL SMF+VAD +++ +
Sbjct: 182 ILHNLHLSETVLKKLIDRNGE-FVDSLTRVMRQGSYDSRAYSIMLLKSMFDVADPMRMIS 241

Query: 248 LTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDS 307
           L  +F+ E++QIL+DQIS QASK+AL+LL+ +    RNR+KAV+AGAV VLI+LLLD   
Sbjct: 242 LRTEFYAELVQILRDQISPQASKAALQLLVQLCPWGRNRIKAVEAGAVAVLIELLLD--- 301

Query: 308 SENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIA 367
           S  +R CEMIL  LD +CGCA+GRAEL+ HAAG+AVVSK++LR+S +GSEKA+ IL+S+A
Sbjct: 302 SVERRPCEMILTGLDLMCGCAEGRAELIRHAAGLAVVSKRVLRVSHIGSEKAMRILFSVA 361

Query: 368 RFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLR 426
           RFS + +V+ EM+++GVV KLC +LQ V  G K KEKA+EIL+LH RTWRNS C+P  L 
Sbjct: 362 RFSATPAVLAEMLQLGVVAKLCLVLQ-VDCGGKTKEKAREILRLHARTWRNSPCVPMRLL 418

BLAST of MELO3C008784.2 vs. TrEMBL
Match: tr|A0A1U8I6M8|A0A1U8I6M8_GOSHI (RING-type E3 ubiquitin transferase OS=Gossypium hirsutum OX=3635 GN=LOC107891193 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 3.7e-131
Identity = 244/415 (58.80%), Postives = 318/415 (76.63%), Query Frame = 0

Query: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70
           +D PP+F+CPISL+IMKDPVT+++GITYDRESIEKWLFS K+ TCPV+  V++D ++TPN
Sbjct: 4   IDVPPFFICPISLEIMKDPVTLSTGITYDRESIEKWLFSGKNTTCPVTKQVIADCELTPN 63

Query: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAA 130
           HTLRR++Q+WC +NAS G+ERIPTPKPP+ + Q+V++L DAK SP  Q  CL  L+SIAA
Sbjct: 64  HTLRRLLQSWCMLNASLGIERIPTPKPPISKAQIVKLLIDAK-SPQQQIKCLDTLKSIAA 123

Query: 131 ANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQISE 190
            N +NKRCMES+G VEFLA +VCN          DY FD  S    DEAL IL+ LQ+SE
Sbjct: 124 ENATNKRCMESSGAVEFLASIVCNYDPTATEESLDYEFD--SIKPIDEALSILYSLQLSE 183

Query: 191 SSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFIEI 250
           +SLK L+  NG  FI +LT+++Q  SY SR+Y+V+LL SM E+AD +Q+ N     FIE+
Sbjct: 184 ASLKNLMGKNGD-FILSLTRVMQRGSYESRAYAVLLLKSMAEIADPVQLINFRPQLFIEL 243

Query: 251 IQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEM 310
           +Q+L DQISKQASK+ L+LL+++    RN++KA +AG VPVLIDLL  LDSS  +R CEM
Sbjct: 244 VQVLHDQISKQASKATLQLLVSICPWGRNKIKAAEAGVVPVLIDLL--LDSSSERRACEM 303

Query: 311 ILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVV 370
           IL +LD +CGCA+GR+ELL H AG+A+VSKKILR+S V SE+AV IL SIA+F  +  V+
Sbjct: 304 ILTVLDAVCGCAEGRSELLKHGAGLAIVSKKILRVSQVASERAVRILLSIAKFGATSKVL 363

Query: 371 QEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
           QEM ++GVV KLC +LQ +  G K KEKA+E+LKLH R W+NS C+P+ L S+YP
Sbjct: 364 QEMQQLGVVAKLCLVLQ-LDCGSKTKEKAREVLKLHARVWKNSPCMPTNLISSYP 411

BLAST of MELO3C008784.2 vs. TrEMBL
Match: tr|A0A0D2QAB1|A0A0D2QAB1_GOSRA (RING-type E3 ubiquitin transferase OS=Gossypium raimondii OX=29730 GN=B456_006G148900 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.1e-130
Identity = 243/415 (58.55%), Postives = 318/415 (76.63%), Query Frame = 0

Query: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70
           +D PP+F+CPISL+IMKDPVT+++GITYDRESIEKWLFS K+ TCPV+  V++D ++TPN
Sbjct: 4   IDVPPFFICPISLEIMKDPVTLSTGITYDRESIEKWLFSGKNTTCPVTKQVIADCELTPN 63

Query: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAA 130
           HTLRR++Q+WC +NAS G+ERIPTPKPP+ + Q+V++L DAK SP  Q  CL  L+SIAA
Sbjct: 64  HTLRRLLQSWCMLNASLGIERIPTPKPPISKAQIVKLLIDAK-SPQQQIKCLDTLKSIAA 123

Query: 131 ANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQISE 190
            N +NKRCMES+G VEFLA +VCN          DY FD  S    DEAL IL+ LQ+SE
Sbjct: 124 ENATNKRCMESSGAVEFLASIVCNYDPTATEESLDYEFD--SIKPIDEALSILYSLQLSE 183

Query: 191 SSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFIEI 250
           +SLK L+  NG + +S LT+++Q  SY SR+Y+V+LL SM E+AD +Q+ N     FIE+
Sbjct: 184 ASLKNLMGKNGDLILS-LTRVMQRGSYESRAYAVLLLKSMAEIADPMQLINFRPQLFIEL 243

Query: 251 IQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEM 310
           +Q+L DQISKQASK+ L+LL+++    RN++KA +AG VPVLIDLL  LDSS  +R CEM
Sbjct: 244 VQVLHDQISKQASKATLQLLVSICPWGRNKIKAAEAGVVPVLIDLL--LDSSSERRACEM 303

Query: 311 ILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVV 370
           IL +LD +CGCA+GR+ELL H AG+A+VSKKILR+S V SE+AV IL SIA+F  +  V+
Sbjct: 304 ILTVLDAVCGCAEGRSELLKHGAGLAIVSKKILRVSQVASERAVRILLSIAKFCATSKVL 363

Query: 371 QEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
           QEM ++GVV KLC +LQ +  G K KEKA+E+LKLH R W+NS C+P+ L S+YP
Sbjct: 364 QEMQQLGVVAKLCLVLQ-LDCGSKTKEKAREVLKLHARVWKNSPCMPTNLISSYP 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008441961.16.0e-234100.00PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo][more]
XP_011653475.11.5e-21692.97PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis sativus] >KGN53961.1 ... [more]
XP_022978192.12.4e-19082.44E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
XP_023543358.13.1e-19082.67E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo][more]
XP_022950790.12.2e-18881.97E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G52450.11.4e-11550.46plant U-box 22[more]
AT2G35930.11.4e-11249.64plant U-box 23[more]
AT3G11840.13.1e-6736.32plant U-box 24[more]
AT3G19380.13.0e-4632.78plant U-box 25[more]
AT1G49780.15.7e-4532.16plant U-box 26[more]
Match NameE-valueIdentityDescription
sp|Q9SVC6|PUB22_ARATH2.5e-11450.46E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
sp|Q84TG3|PUB23_ARATH2.6e-11149.64E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
sp|Q9SF15|PUB24_ARATH9.6e-6636.40E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
sp|Q9LT79|PUB25_ARATH5.5e-4532.78U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
sp|Q9FXA4|PUB26_ARATH1.0e-4332.16U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3B4K2|A0A1S3B4K2_CUCME4.0e-234100.00RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 ... [more]
tr|A0A0A0L1F5|A0A0A0L1F5_CUCSA9.9e-21792.97RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE... [more]
tr|W9SK34|W9SK34_9ROSA2.9e-13158.49RING-type E3 ubiquitin transferase OS=Morus notabilis OX=981085 GN=L484_016918 P... [more]
tr|A0A1U8I6M8|A0A1U8I6M8_GOSHI3.7e-13158.80RING-type E3 ubiquitin transferase OS=Gossypium hirsutum OX=3635 GN=LOC107891193... [more]
tr|A0A0D2QAB1|A0A0D2QAB1_GOSRA1.1e-13058.55RING-type E3 ubiquitin transferase OS=Gossypium raimondii OX=29730 GN=B456_006G1... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0004842ubiquitin-protein transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: INTERPRO
TermDefinition
IPR016024ARM-type_fold
IPR011989ARM-like
IPR013083Znf_RING/FYVE/PHD
IPR003613Ubox_domain
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0016740 transferase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0005488 binding
molecular_function GO:0016874 ligase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C008784.2.1MELO3C008784.2.1mRNA


Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 16..80
e-value: 5.5E-33
score: 125.6
IPR003613U box domainPFAMPF04564U-boxcoord: 14..83
e-value: 8.6E-20
score: 70.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 12..87
score: 41.992
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 1..92
e-value: 4.0E-28
score: 99.3
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 98..406
e-value: 9.2E-14
score: 52.9
NoneNo IPR availablePANTHERPTHR22849:SF54SUBFAMILY NOT NAMEDcoord: 12..425
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 12..425
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 17..59
e-value: 1.8221E-20
score: 83.437
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 8..87
IPR016024Armadillo-type foldSUPERFAMILYSSF48371ARM repeatcoord: 104..387