MELO3C008784 (gene) Melon (DHL92) v3.5.1

NameMELO3C008784
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionU-box domain-containing family protein
Locationchr8 : 24962350 .. 24963919 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTTCATTTCAAAACCTAACGTTTTCACAACTTCCCAACAACTCTTTTCTCTCATCTGATCTCCCTCTAACAACCCTTTCTCTTCCTCTAAAGATCATTCTATTATTTCTCTAGCTAGTTGAGCCATTCATGGCAGAATTAGATGATCAAGATCAAGTAGTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTGCAAATCATGAAAGATCCCGTTACCATCGCCTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCCGACAAACACAAGACATGCCCCGTCTCGCACATAGTTTTGTCAGACTTTGACGTCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGTCAATGCTTCTAAAGGTGTAGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTTGTTCGGATTCTTGCCGATGCTAAGTTATCTCCATTCTCTCAAAAGAATTGCCTTTGTCGCCTTCGATCTATTGCTGCGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATTCTACCACCAACATGGAGTATGGCTTAGAAGGTAATATTCAAAACTCAAAACAAATTTAAAGTTTCTTATTTTGCATAGTTTGAACACTACGAACCCCTCATTGCCAATAATATATGTGTAGTTATAATGTATAAAATAAGCTTTGAGAATTTAATAATATTGGTTATTTATGCATATTTGATGATGCAGATTACGCGTTTGACGACATGTCGGCAAATTCAGCCGATGAGGCTTTGATTATTCTTCATAAACTCCAAATCTCCGAATCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTATTCCTCTCGATCATATTCAGTAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATCCAAAATCTCACCGCCGATTTCTTTATCGAAATCATCCAAATCCTCAAAGATCAAATCTCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCAGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTCGATCAGCTCTGTGGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCATGCGGCGGGGATTGCCGTGGTGTCGAAGAAGATTCTGAGAATTTCGACGGTTGGAAGCGAGAAAGCGGTTGGGATCTTGTGGTCTATAGCGAGATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGAGGATTGGTGTTGTGACGAAGCTGTGTTTTCTGCTTCAGGTGGTGGGCGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCGTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAA

mRNA sequence

CTCTTCATTTCAAAACCTAACGTTTTCACAACTTCCCAACAACTCTTTTCTCTCATCTGATCTCCCTCTAACAACCCTTTCTCTTCCTCTAAAGATCATTCTATTATTTCTCTAGCTAGTTGAGCCATTCATGGCAGAATTAGATGATCAAGATCAAGTAGTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTGCAAATCATGAAAGATCCCGTTACCATCGCCTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCCGACAAACACAAGACATGCCCCGTCTCGCACATAGTTTTGTCAGACTTTGACGTCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGTCAATGCTTCTAAAGGTGTAGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTTGTTCGGATTCTTGCCGATGCTAAGTTATCTCCATTCTCTCAAAAGAATTGCCTTTGTCGCCTTCGATCTATTGCTGCGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATTCTACCACCAACATGGAGTATGGCTTAGAAGATTACGCGTTTGACGACATGTCGGCAAATTCAGCCGATGAGGCTTTGATTATTCTTCATAAACTCCAAATCTCCGAATCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTATTCCTCTCGATCATATTCAGTAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATCCAAAATCTCACCGCCGATTTCTTTATCGAAATCATCCAAATCCTCAAAGATCAAATCTCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCAGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTCGATCAGCTCTGTGGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCATGCGGCGGGGATTGCCGTGGTGTCGAAGAAGATTCTGAGAATTTCGACGGTTGGAAGCGAGAAAGCGGTTGGGATCTTGTGGTCTATAGCGAGATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGAGGATTGGTGTTGTGACGAAGCTGTGTTTTCTGCTTCAGGTGGTGGGCGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCGTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAA

Coding sequence (CDS)

ATGGCAGAATTAGATGATCAAGATCAAGTAGTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTGCAAATCATGAAAGATCCCGTTACCATCGCCTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCCGACAAACACAAGACATGCCCCGTCTCGCACATAGTTTTGTCAGACTTTGACGTCACTCCCAACCATACTTTAAGACGAGTCATTCAAGCTTGGTGCACCGTCAATGCTTCTAAAGGTGTAGAGAGGATACCGACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTTGTTCGGATTCTTGCCGATGCTAAGTTATCTCCATTCTCTCAAAAGAATTGCCTTTGTCGCCTTCGATCTATTGCTGCGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATTCTACCACCAACATGGAGTATGGCTTAGAAGATTACGCGTTTGACGACATGTCGGCAAATTCAGCCGATGAGGCTTTGATTATTCTTCATAAACTCCAAATCTCCGAATCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTACAAAATCGAAGCTATTCCTCTCGATCATATTCAGTAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATCCAAAATCTCACCGCCGATTTCTTTATCGAAATCATCCAAATCCTCAAAGATCAAATCTCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCAGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCGTCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTCGATCAGCTCTGTGGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCATGCGGCGGGGATTGCCGTGGTGTCGAAGAAGATTCTGAGAATTTCGACGGTTGGAAGCGAGAAAGCGGTTGGGATCTTGTGGTCTATAGCGAGATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGAGGATTGGTGTTGTGACGAAGCTGTGTTTTCTGCTTCAGGTGGTGGGCGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCGTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAA

Protein sequence

MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYPQ*
BLAST of MELO3C008784 vs. Swiss-Prot
Match: PUB22_ARATH (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana GN=PUB22 PE=1 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 3.1e-117
Identity = 219/434 (50.46%), Postives = 300/434 (69.12%), Query Frame = 1

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           DQ ++ P +FLCPISL IMKDPV +++GITYDRESIEKWLFS K  +CPV+  V+++ D+
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDL 61

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS G+ERIPTPKPP+ + ++ +++ ++  S  +Q  CL RLR 
Sbjct: 62  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 121

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCN------------STTNMEYGLEDYAFD---DMS 187
           I + N +NKRC+E+A V EFLA +V N            S++N+    +    +   D S
Sbjct: 122 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 181

Query: 188 ANSADEALIILHKLQISESSLKFLLSNNGGI-FISTLTKILQNRSYSSRSYSVMLLNSMF 247
            +  DEAL +L+ L  SE++LK LL+N  G   + TLTKI+Q   Y SR+Y+ +LL  + 
Sbjct: 182 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 241

Query: 248 EVADQIQIQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPV 307
           EVAD +QI  L  + F E+IQIL DQIS +A++SA+++L+      RNR KAV+ G + +
Sbjct: 242 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 301

Query: 308 LIDLLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSE 367
           +I+LL+D   S  +R  EM +V+LD LC CA+GRAE LNH A IAVVSKKILR+S + SE
Sbjct: 302 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 361

Query: 368 KAVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWR 426
           +AV +L S+ RF  + S++QEM+++GVV KLC +LQ V  G K KEKAKE+LKLH R WR
Sbjct: 362 RAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWR 421

BLAST of MELO3C008784 vs. Swiss-Prot
Match: PUB23_ARATH (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana GN=PUB23 PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 4.2e-114
Identity = 208/419 (49.64%), Postives = 293/419 (69.93%), Query Frame = 1

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           D+ ++ PP+FLCPISL+IMKDPV +++GITYDR+SIEKWLF+ K  +CPV+   ++D D+
Sbjct: 7   DEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADL 66

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS GVERIPTP+PP+ + ++ +++ D+  S  +Q  CL RLR 
Sbjct: 67  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 126

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQ 187
           I + N +NKRC+E+AGV EFLA +V N + N               +  DEAL +L+ L+
Sbjct: 127 IVSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLE 186

Query: 188 ISESSLKFLLSN-NGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADF 247
            SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q   L  + 
Sbjct: 187 TSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV 246

Query: 248 FIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKR 307
           F E++QIL D+IS++A+K+A+ +L+ +    RNR KAV+AG + V+I+LL+D   +  +R
Sbjct: 247 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 306

Query: 308 LCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGS 367
             EM +V+LD LC CA+GRAE LNH A IAVV KKILR+S   S++AV +L S+ RF  +
Sbjct: 307 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 366

Query: 368 QSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
            +++ EM+++GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP  +  AYP
Sbjct: 367 PALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of MELO3C008784 vs. Swiss-Prot
Match: PUB24_ARATH (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana GN=PUB24 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 8.0e-65
Identity = 164/456 (35.96%), Postives = 248/456 (54.39%), Query Frame = 1

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           +D +++ ++ P YF+CPISL+IMKDPVT  SGITYDR++I KWL  +K  +CPV+   L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 64  -DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKL---SPFSQK 123
            D D+TPNH LRR+IQ WC  N ++GV RI TP+ P  +  VV  + + K        ++
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 124 NCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEA 183
             L +L  + A + +N+R M   GV + L   V   T+  E G              DE+
Sbjct: 121 ETLQKLE-VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRR-------RIKGLDES 180

Query: 184 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSM--------- 243
           L +LH + I  +  K +L  N  + + +LT +L    + S++Y+++LL ++         
Sbjct: 181 LRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIV 240

Query: 244 -------FE-----VADQIQIQNLTADFFIEIIQ--------------ILKDQISKQASK 303
                  F+     + D +   N T+    E +Q              +    + KQA  
Sbjct: 241 ERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVT 300

Query: 304 SALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDQLCGCADG 363
           +AL +L+  SS  RNR   V  GAV  LI+L  ++  +  KR+ E++L +L +LC CA+G
Sbjct: 301 AALMILLETSSWSRNRSLLVDLGAVSELIEL--EISYTGEKRITELMLGVLSRLCCCANG 360

Query: 364 RAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVVQEMVRIGVVTKLCF 421
           RAE+L H  GIAVV+K++LR+S    ++A+ IL ++++FS    VV+EMV +G V KLC 
Sbjct: 361 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 420

BLAST of MELO3C008784 vs. Swiss-Prot
Match: PUB25_ARATH (U-box domain-containing protein 25 OS=Arabidopsis thaliana GN=PUB25 PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 8.9e-48
Identity = 137/418 (32.78%), Postives = 215/418 (51.44%), Query Frame = 1

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           ++  D  +  P +F CPISL++M+DPVT+ +G TYDR SIE W+    + TCPV+   LS
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   L RLR  A  ++ N+  + +    E L  ++ + TT+ E                 
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSEL--------------VS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L I+E +    +S++ G  +  LT++L + S  +R  +  L+  +       
Sbjct: 185 ESLALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 244

Query: 244 QIQNLTAD---FFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++   ++    F  ++ +L++ I S++A K  +K L A+ S    R  A+ AGA  +LI
Sbjct: 245 DLKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTW 411
           A G L  +A  +  +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 365 AAGAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C008784 vs. Swiss-Prot
Match: PUB26_ARATH (U-box domain-containing protein 26 OS=Arabidopsis thaliana GN=PUB26 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.3e-46
Identity = 137/426 (32.16%), Postives = 217/426 (50.94%), Query Frame = 1

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           L+  D  +  P +F CPISL +M DPVTI++G TYDR SI+ W+    + TCPV+ + LS
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMG-NTTCPVTRVALS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   + RLR +A  +E N+  +      E L  ++            D     +S+    
Sbjct: 125 RAAAIRRLRGLARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L ++E+  + + S+     +  +T++L + S   R  +  L+  +   A  +
Sbjct: 185 ESLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSM 244

Query: 244 QIQNLTA---DFFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++ + +     F  ++ +LK+ I S++A K  +K + A+    + R  A+ AGA  +LI
Sbjct: 245 DLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRN 419
           A G L  +A  +  +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W +
Sbjct: 365 AAGAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPD 410

BLAST of MELO3C008784 vs. TrEMBL
Match: A0A0A0L1F5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G194780 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 6.8e-220
Identity = 397/427 (92.97%), Postives = 410/427 (96.02%), Query Frame = 1

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 180

Query: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240
           L+ILHKLQ+SESSLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMF+VADQIQI
Sbjct: 181 LLILHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQI 240

Query: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 360

Query: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 421 LRSAYPQ 427
           LRSAYPQ
Sbjct: 421 LRSAYPQ 424

BLAST of MELO3C008784 vs. TrEMBL
Match: W9SK34_9ROSA (E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_016918 PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.0e-134
Identity = 248/424 (58.49%), Postives = 331/424 (78.07%), Query Frame = 1

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           D  +D PP+FLCPISL+IMKDPVT+ +GITYDRESIEKWLFS K+ TCPV+  VLSD D+
Sbjct: 2   DTEIDVPPFFLCPISLEIMKDPVTVLTGITYDRESIEKWLFSGKNDTCPVTKQVLSDSDL 61

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQAWCT+NA+ G+ERIPTPKPP+ + Q+ ++L+DAK SP SQ  CL RLRS
Sbjct: 62  TPNHTLRRLIQAWCTMNAALGIERIPTPKPPISKSQIAKLLSDAK-SPQSQIKCLRRLRS 121

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCNS------TTNMEYGLEDYAFDDMSANSADEALI 187
           IAA +E+NKRC+ESAG VEFL  ++ N       ++ +     D +F D + +++DEAL 
Sbjct: 122 IAAESETNKRCLESAGAVEFLVSLIANDNNENAVSSPLSSSENDESF-DFARSASDEALT 181

Query: 188 ILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQN 247
           ILH L +SE+ LK L+  NG  F+ +LT++++  SY SR+YS+MLL SMF+VAD +++ +
Sbjct: 182 ILHNLHLSETVLKKLIDRNGE-FVDSLTRVMRQGSYDSRAYSIMLLKSMFDVADPMRMIS 241

Query: 248 LTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDS 307
           L  +F+ E++QIL+DQIS QASK+AL+LL+ +    RNR+KAV+AGAV VLI+LLLD   
Sbjct: 242 LRTEFYAELVQILRDQISPQASKAALQLLVQLCPWGRNRIKAVEAGAVAVLIELLLD--- 301

Query: 308 SENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIA 367
           S  +R CEMIL  LD +CGCA+GRAEL+ HAAG+AVVSK++LR+S +GSEKA+ IL+S+A
Sbjct: 302 SVERRPCEMILTGLDLMCGCAEGRAELIRHAAGLAVVSKRVLRVSHIGSEKAMRILFSVA 361

Query: 368 RFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLR 426
           RFS + +V+ EM+++GVV KLC +LQ V  G K KEKA+EIL+LH RTWRNS C+P  L 
Sbjct: 362 RFSATPAVLAEMLQLGVVAKLCLVLQ-VDCGGKTKEKAREILRLHARTWRNSPCVPMRLL 418

BLAST of MELO3C008784 vs. TrEMBL
Match: A0A0D2QAB1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G148900 PE=4 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 9.7e-134
Identity = 243/415 (58.55%), Postives = 318/415 (76.63%), Query Frame = 1

Query: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70
           +D PP+F+CPISL+IMKDPVT+++GITYDRESIEKWLFS K+ TCPV+  V++D ++TPN
Sbjct: 4   IDVPPFFICPISLEIMKDPVTLSTGITYDRESIEKWLFSGKNTTCPVTKQVIADCELTPN 63

Query: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAA 130
           HTLRR++Q+WC +NAS G+ERIPTPKPP+ + Q+V++L DAK SP  Q  CL  L+SIAA
Sbjct: 64  HTLRRLLQSWCMLNASLGIERIPTPKPPISKAQIVKLLIDAK-SPQQQIKCLDTLKSIAA 123

Query: 131 ANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQISE 190
            N +NKRCMES+G VEFLA +VCN          DY FD  S    DEAL IL+ LQ+SE
Sbjct: 124 ENATNKRCMESSGAVEFLASIVCNYDPTATEESLDYEFD--SIKPIDEALSILYSLQLSE 183

Query: 191 SSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFIEI 250
           +SLK L+  NG + +S LT+++Q  SY SR+Y+V+LL SM E+AD +Q+ N     FIE+
Sbjct: 184 ASLKNLMGKNGDLILS-LTRVMQRGSYESRAYAVLLLKSMAEIADPMQLINFRPQLFIEL 243

Query: 251 IQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEM 310
           +Q+L DQISKQASK+ L+LL+++    RN++KA +AG VPVLIDLL  LDSS  +R CEM
Sbjct: 244 VQVLHDQISKQASKATLQLLVSICPWGRNKIKAAEAGVVPVLIDLL--LDSSSERRACEM 303

Query: 311 ILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVV 370
           IL +LD +CGCA+GR+ELL H AG+A+VSKKILR+S V SE+AV IL SIA+F  +  V+
Sbjct: 304 ILTVLDAVCGCAEGRSELLKHGAGLAIVSKKILRVSQVASERAVRILLSIAKFCATSKVL 363

Query: 371 QEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
           QEM ++GVV KLC +LQ +  G K KEKA+E+LKLH R W+NS C+P+ L S+YP
Sbjct: 364 QEMQQLGVVAKLCLVLQ-LDCGSKTKEKAREVLKLHARVWKNSPCMPTNLISSYP 411

BLAST of MELO3C008784 vs. TrEMBL
Match: A0A067KIQ1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08750 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 4.5e-131
Identity = 250/418 (59.81%), Postives = 322/418 (77.03%), Query Frame = 1

Query: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70
           +D PP+FLCPISLQIMKDPVT+ +GITYDRESIEKWLFS K+ TCPV+  V+SD ++TPN
Sbjct: 4   IDVPPFFLCPISLQIMKDPVTVPTGITYDRESIEKWLFSGKNNTCPVTKQVISDCELTPN 63

Query: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKL-SPFSQKNCLCRLRSIA 130
            TL R+IQ+WCT+NAS G+ERIPTPKPPV + Q+ +++ DA + SP  Q  CL +LRS+A
Sbjct: 64  VTLMRLIQSWCTLNASHGIERIPTPKPPVSKAQISKLIKDATMSSPQQQMKCLKKLRSMA 123

Query: 131 AANESNKRCMESAGVVEFLAGVV--CNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQ 190
             N++NKRCMESAGVVEFLA +V   NS +  EY   +    + + + +DEAL  L+ LQ
Sbjct: 124 NVNDTNKRCMESAGVVEFLASIVNTYNSLSFEEYSSSNLEEINEAISLSDEALSFLYGLQ 183

Query: 191 ISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFF 250
           +SES LK L+S N  I I +LT+IL+N +Y SR+YSV+LL S+ EVAD IQ+ NL  + F
Sbjct: 184 LSESGLKVLISKNAEI-IDSLTRILKNGNYESRAYSVLLLKSILEVADPIQLINLRQELF 243

Query: 251 IEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRL 310
           +EI+QIL+DQIS QA+KS+L+LL+ +    RNRVKAV A AV VLI+LLLD   S  KR+
Sbjct: 244 VEIVQILRDQISHQATKSSLRLLVNLCPWGRNRVKAVAANAVSVLINLLLD---SSEKRV 303

Query: 311 CEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQ 370
           CEM L++LD +C CA+GRAELL H AG+AVVSKKILR+S V SEKAV IL SI++FS + 
Sbjct: 304 CEMALMVLDLVCQCAEGRAELLGHGAGLAVVSKKILRVSQVASEKAVRILLSISKFSATN 363

Query: 371 SVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
           +V+QEM++IGVV KLC +LQ V  G+K KEKA+E+LKLH R W+NS C+P+ L S YP
Sbjct: 364 NVLQEMLQIGVVAKLCLVLQ-VDCGIKNKEKAREVLKLHARAWKNSPCIPANLLSFYP 416

BLAST of MELO3C008784 vs. TrEMBL
Match: A0A061EVP3_THECC (Plant U-box 23 OS=Theobroma cacao GN=TCM_022966 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 5.9e-131
Identity = 237/417 (56.83%), Postives = 324/417 (77.70%), Query Frame = 1

Query: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70
           +D PP+F+CPISL+IMKDPVT+++GITYDRESIEKWLFS K+ TCPV+  V++D D+TPN
Sbjct: 4   IDVPPFFICPISLEIMKDPVTVSTGITYDRESIEKWLFSGKNTTCPVTKQVITDCDLTPN 63

Query: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAA 130
           HTLRR+IQ+WC +NAS G+ERIPTPKPP+ + Q+ ++L DA  SP  Q +CL RL+SIA+
Sbjct: 64  HTLRRLIQSWCMLNASHGIERIPTPKPPISKAQITKLLNDA-TSPHQQISCLRRLQSIAS 123

Query: 131 ANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDD--MSANSADEALIILHKLQI 190
            N +NKRCMES+G VEFLA +V    +N ++   + +FD+   S    DEAL IL+ LQ+
Sbjct: 124 ENTTNKRCMESSGAVEFLASIV----SNYDFTAAEESFDNEFESTRPTDEALSILYNLQL 183

Query: 191 SESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFI 250
           SE++LK L+  NG  FI +LT+++Q  SY SR+Y+++LL SM E+AD +Q+ +L  + F+
Sbjct: 184 SEAALKNLMRKNGD-FIVSLTRVMQRGSYESRAYAILLLKSMLEIADPMQLISLRPELFV 243

Query: 251 EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLC 310
           E+IQ+L+DQIS+QASK+ L+LL+++S   RN++KA +AGAV VLIDLLLD   S  +R C
Sbjct: 244 ELIQVLRDQISQQASKATLQLLVSISPWGRNKIKAAEAGAVSVLIDLLLD---SSERRAC 303

Query: 311 EMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQS 370
           EMIL +LD LCGCA+GRAELL H AG+A+VSKKILR+S V SE+AV IL SI++F  + +
Sbjct: 304 EMILTVLDALCGCAEGRAELLRHGAGLAIVSKKILRVSLVASERAVRILLSISKFCATSN 363

Query: 371 VVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
           V+ EM+++GVV KLC +LQ V  G K KE+A+E+LKLH + W+NS C+P  L S+YP
Sbjct: 364 VLHEMLQLGVVAKLCLVLQ-VDCGYKTKERAREVLKLHAKAWKNSPCIPINLLSSYP 410

BLAST of MELO3C008784 vs. TAIR10
Match: AT3G52450.1 (AT3G52450.1 plant U-box 22)

HSP 1 Score: 423.3 bits (1087), Expect = 1.8e-118
Identity = 219/434 (50.46%), Postives = 300/434 (69.12%), Query Frame = 1

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           DQ ++ P +FLCPISL IMKDPV +++GITYDRESIEKWLFS K  +CPV+  V+++ D+
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDL 61

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS G+ERIPTPKPP+ + ++ +++ ++  S  +Q  CL RLR 
Sbjct: 62  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 121

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCN------------STTNMEYGLEDYAFD---DMS 187
           I + N +NKRC+E+A V EFLA +V N            S++N+    +    +   D S
Sbjct: 122 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 181

Query: 188 ANSADEALIILHKLQISESSLKFLLSNNGGI-FISTLTKILQNRSYSSRSYSVMLLNSMF 247
            +  DEAL +L+ L  SE++LK LL+N  G   + TLTKI+Q   Y SR+Y+ +LL  + 
Sbjct: 182 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 241

Query: 248 EVADQIQIQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPV 307
           EVAD +QI  L  + F E+IQIL DQIS +A++SA+++L+      RNR KAV+ G + +
Sbjct: 242 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 301

Query: 308 LIDLLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSE 367
           +I+LL+D   S  +R  EM +V+LD LC CA+GRAE LNH A IAVVSKKILR+S + SE
Sbjct: 302 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 361

Query: 368 KAVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWR 426
           +AV +L S+ RF  + S++QEM+++GVV KLC +LQ V  G K KEKAKE+LKLH R WR
Sbjct: 362 RAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWR 421

BLAST of MELO3C008784 vs. TAIR10
Match: AT2G35930.1 (AT2G35930.1 plant U-box 23)

HSP 1 Score: 412.9 bits (1060), Expect = 2.4e-115
Identity = 208/419 (49.64%), Postives = 293/419 (69.93%), Query Frame = 1

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           D+ ++ PP+FLCPISL+IMKDPV +++GITYDR+SIEKWLF+ K  +CPV+   ++D D+
Sbjct: 7   DEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADL 66

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQ+WCT+NAS GVERIPTP+PP+ + ++ +++ D+  S  +Q  CL RLR 
Sbjct: 67  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 126

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQ 187
           I + N +NKRC+E+AGV EFLA +V N + N               +  DEAL +L+ L+
Sbjct: 127 IVSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLE 186

Query: 188 ISESSLKFLLSN-NGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADF 247
            SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q   L  + 
Sbjct: 187 TSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV 246

Query: 248 FIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKR 307
           F E++QIL D+IS++A+K+A+ +L+ +    RNR KAV+AG + V+I+LL+D   +  +R
Sbjct: 247 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 306

Query: 308 LCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGS 367
             EM +V+LD LC CA+GRAE LNH A IAVV KKILR+S   S++AV +L S+ RF  +
Sbjct: 307 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 366

Query: 368 QSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
            +++ EM+++GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP  +  AYP
Sbjct: 367 PALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of MELO3C008784 vs. TAIR10
Match: AT3G11840.1 (AT3G11840.1 plant U-box 24)

HSP 1 Score: 250.0 bits (637), Expect = 2.7e-66
Identity = 164/457 (35.89%), Postives = 249/457 (54.49%), Query Frame = 1

Query: 3   ELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVL 62
           ++D +++ ++ P YF+CPISL+IMKDPVT  SGITYDR++I KWL  +K  +CPV+   L
Sbjct: 14  QMDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPL 73

Query: 63  S-DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKL---SPFSQ 122
             D D+TPNH LRR+IQ WC  N ++GV RI TP+ P  +  VV  + + K        +
Sbjct: 74  PLDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGR 133

Query: 123 KNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADE 182
           +  L +L  + A + +N+R M   GV + L   V   T+  E G              DE
Sbjct: 134 EETLQKLE-VLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRR-------RIKGLDE 193

Query: 183 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSM-------- 242
           +L +LH + I  +  K +L  N  + + +LT +L    + S++Y+++LL ++        
Sbjct: 194 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 253

Query: 243 --------FE-----VADQIQIQNLTADFFIEIIQ--------------ILKDQISKQAS 302
                   F+     + D +   N T+    E +Q              +    + KQA 
Sbjct: 254 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 313

Query: 303 KSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDQLCGCAD 362
            +AL +L+  SS  RNR   V  GAV  LI+L  ++  +  KR+ E++L +L +LC CA+
Sbjct: 314 TAALMILLETSSWSRNRSLLVDLGAVSELIEL--EISYTGEKRITELMLGVLSRLCCCAN 373

Query: 363 GRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVVQEMVRIGVVTKLC 421
           GRAE+L H  GIAVV+K++LR+S    ++A+ IL ++++FS    VV+EMV +G V KLC
Sbjct: 374 GRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLC 433

BLAST of MELO3C008784 vs. TAIR10
Match: AT3G19380.1 (AT3G19380.1 plant U-box 25)

HSP 1 Score: 192.6 bits (488), Expect = 5.0e-49
Identity = 137/418 (32.78%), Postives = 215/418 (51.44%), Query Frame = 1

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           ++  D  +  P +F CPISL++M+DPVT+ +G TYDR SIE W+    + TCPV+   LS
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   L RLR  A  ++ N+  + +    E L  ++ + TT+ E                 
Sbjct: 125 RAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSEL--------------VS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L I+E +    +S++ G  +  LT++L + S  +R  +  L+  +       
Sbjct: 185 ESLALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 244

Query: 244 QIQNLTAD---FFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++   ++    F  ++ +L++ I S++A K  +K L A+ S    R  A+ AGA  +LI
Sbjct: 245 DLKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTW 411
           A G L  +A  +  +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 365 AAGAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C008784 vs. TAIR10
Match: AT1G49780.1 (AT1G49780.1 plant U-box 26)

HSP 1 Score: 188.7 bits (478), Expect = 7.3e-48
Identity = 137/426 (32.16%), Postives = 217/426 (50.94%), Query Frame = 1

Query: 4   LDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLS 63
           L+  D  +  P +F CPISL +M DPVTI++G TYDR SI+ W+    + TCPV+ + LS
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMG-NTTCPVTRVALS 64

Query: 64  DFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAK------LSPFS 123
           DF + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A       +S  S
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRS 124

Query: 124 QKNCLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSAD 183
           +   + RLR +A  +E N+  +      E L  ++            D     +S+    
Sbjct: 125 RAAAIRRLRGLARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVS 184

Query: 184 EALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQI 243
           E+L +L  L ++E+  + + S+     +  +T++L + S   R  +  L+  +   A  +
Sbjct: 185 ESLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSM 244

Query: 244 QIQNLTA---DFFIEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLI 303
            ++ + +     F  ++ +LK+ I S++A K  +K + A+    + R  A+ AGA  +LI
Sbjct: 245 DLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILI 304

Query: 304 D-LLLDLDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEK 363
           D L  D D  + +R     L  ++ LC   +G A    HA  + ++ K ILR+S   +E 
Sbjct: 305 DRLAADFDRCDTER----GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEY 364

Query: 364 AVGILWSIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRN 419
           A G L  +A  +  +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W +
Sbjct: 365 AAGAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPD 410

BLAST of MELO3C008784 vs. NCBI nr
Match: gi|659082653|ref|XP_008441961.1| (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo])

HSP 1 Score: 828.9 bits (2140), Expect = 3.9e-237
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 1

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180
           CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240
           IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300
           NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360
           SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420
           ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSAYPQ 427
           RSAYPQ
Sbjct: 421 RSAYPQ 426

BLAST of MELO3C008784 vs. NCBI nr
Match: gi|778692549|ref|XP_011653475.1| (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis sativus])

HSP 1 Score: 771.2 bits (1990), Expect = 9.7e-220
Identity = 397/427 (92.97%), Postives = 410/427 (96.02%), Query Frame = 1

Query: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 60

Query: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 120

Query: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 180

Query: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240
           L+ILHKLQ+SESSLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMF+VADQIQI
Sbjct: 181 LLILHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQI 240

Query: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 360

Query: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 421 LRSAYPQ 427
           LRSAYPQ
Sbjct: 421 LRSAYPQ 424

BLAST of MELO3C008784 vs. NCBI nr
Match: gi|703139476|ref|XP_010106989.1| (E3 ubiquitin-protein ligase [Morus notabilis])

HSP 1 Score: 487.3 bits (1253), Expect = 2.8e-134
Identity = 248/424 (58.49%), Postives = 331/424 (78.07%), Query Frame = 1

Query: 8   DQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDV 67
           D  +D PP+FLCPISL+IMKDPVT+ +GITYDRESIEKWLFS K+ TCPV+  VLSD D+
Sbjct: 2   DTEIDVPPFFLCPISLEIMKDPVTVLTGITYDRESIEKWLFSGKNDTCPVTKQVLSDSDL 61

Query: 68  TPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRS 127
           TPNHTLRR+IQAWCT+NA+ G+ERIPTPKPP+ + Q+ ++L+DAK SP SQ  CL RLRS
Sbjct: 62  TPNHTLRRLIQAWCTMNAALGIERIPTPKPPISKSQIAKLLSDAK-SPQSQIKCLRRLRS 121

Query: 128 IAAANESNKRCMESAGVVEFLAGVVCNS------TTNMEYGLEDYAFDDMSANSADEALI 187
           IAA +E+NKRC+ESAG VEFL  ++ N       ++ +     D +F D + +++DEAL 
Sbjct: 122 IAAESETNKRCLESAGAVEFLVSLIANDNNENAVSSPLSSSENDESF-DFARSASDEALT 181

Query: 188 ILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQN 247
           ILH L +SE+ LK L+  NG  F+ +LT++++  SY SR+YS+MLL SMF+VAD +++ +
Sbjct: 182 ILHNLHLSETVLKKLIDRNGE-FVDSLTRVMRQGSYDSRAYSIMLLKSMFDVADPMRMIS 241

Query: 248 LTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDS 307
           L  +F+ E++QIL+DQIS QASK+AL+LL+ +    RNR+KAV+AGAV VLI+LLLD   
Sbjct: 242 LRTEFYAELVQILRDQISPQASKAALQLLVQLCPWGRNRIKAVEAGAVAVLIELLLD--- 301

Query: 308 SENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIA 367
           S  +R CEMIL  LD +CGCA+GRAEL+ HAAG+AVVSK++LR+S +GSEKA+ IL+S+A
Sbjct: 302 SVERRPCEMILTGLDLMCGCAEGRAELIRHAAGLAVVSKRVLRVSHIGSEKAMRILFSVA 361

Query: 368 RFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLR 426
           RFS + +V+ EM+++GVV KLC +LQ V  G K KEKA+EIL+LH RTWRNS C+P  L 
Sbjct: 362 RFSATPAVLAEMLQLGVVAKLCLVLQ-VDCGGKTKEKAREILRLHARTWRNSPCVPMRLL 418

BLAST of MELO3C008784 vs. NCBI nr
Match: gi|823174256|ref|XP_012485721.1| (PREDICTED: E3 ubiquitin-protein ligase PUB23 [Gossypium raimondii])

HSP 1 Score: 485.0 bits (1247), Expect = 1.4e-133
Identity = 243/415 (58.55%), Postives = 318/415 (76.63%), Query Frame = 1

Query: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70
           +D PP+F+CPISL+IMKDPVT+++GITYDRESIEKWLFS K+ TCPV+  V++D ++TPN
Sbjct: 4   IDVPPFFICPISLEIMKDPVTLSTGITYDRESIEKWLFSGKNTTCPVTKQVIADCELTPN 63

Query: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAA 130
           HTLRR++Q+WC +NAS G+ERIPTPKPP+ + Q+V++L DAK SP  Q  CL  L+SIAA
Sbjct: 64  HTLRRLLQSWCMLNASLGIERIPTPKPPISKAQIVKLLIDAK-SPQQQIKCLDTLKSIAA 123

Query: 131 ANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQISE 190
            N +NKRCMES+G VEFLA +VCN          DY FD  S    DEAL IL+ LQ+SE
Sbjct: 124 ENATNKRCMESSGAVEFLASIVCNYDPTATEESLDYEFD--SIKPIDEALSILYSLQLSE 183

Query: 191 SSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFIEI 250
           +SLK L+  NG + +S LT+++Q  SY SR+Y+V+LL SM E+AD +Q+ N     FIE+
Sbjct: 184 ASLKNLMGKNGDLILS-LTRVMQRGSYESRAYAVLLLKSMAEIADPMQLINFRPQLFIEL 243

Query: 251 IQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEM 310
           +Q+L DQISKQASK+ L+LL+++    RN++KA +AG VPVLIDLL  LDSS  +R CEM
Sbjct: 244 VQVLHDQISKQASKATLQLLVSICPWGRNKIKAAEAGVVPVLIDLL--LDSSSERRACEM 303

Query: 311 ILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVV 370
           IL +LD +CGCA+GR+ELL H AG+A+VSKKILR+S V SE+AV IL SIA+F  +  V+
Sbjct: 304 ILTVLDAVCGCAEGRSELLKHGAGLAIVSKKILRVSQVASERAVRILLSIAKFCATSKVL 363

Query: 371 QEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
           QEM ++GVV KLC +LQ +  G K KEKA+E+LKLH R W+NS C+P+ L S+YP
Sbjct: 364 QEMQQLGVVAKLCLVLQ-LDCGSKTKEKAREVLKLHARVWKNSPCMPTNLISSYP 411

BLAST of MELO3C008784 vs. NCBI nr
Match: gi|802611106|ref|XP_012074309.1| (PREDICTED: E3 ubiquitin-protein ligase PUB22 [Jatropha curcas])

HSP 1 Score: 476.1 bits (1224), Expect = 6.5e-131
Identity = 250/418 (59.81%), Postives = 322/418 (77.03%), Query Frame = 1

Query: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70
           +D PP+FLCPISLQIMKDPVT+ +GITYDRESIEKWLFS K+ TCPV+  V+SD ++TPN
Sbjct: 4   IDVPPFFLCPISLQIMKDPVTVPTGITYDRESIEKWLFSGKNNTCPVTKQVISDCELTPN 63

Query: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKL-SPFSQKNCLCRLRSIA 130
            TL R+IQ+WCT+NAS G+ERIPTPKPPV + Q+ +++ DA + SP  Q  CL +LRS+A
Sbjct: 64  VTLMRLIQSWCTLNASHGIERIPTPKPPVSKAQISKLIKDATMSSPQQQMKCLKKLRSMA 123

Query: 131 AANESNKRCMESAGVVEFLAGVV--CNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQ 190
             N++NKRCMESAGVVEFLA +V   NS +  EY   +    + + + +DEAL  L+ LQ
Sbjct: 124 NVNDTNKRCMESAGVVEFLASIVNTYNSLSFEEYSSSNLEEINEAISLSDEALSFLYGLQ 183

Query: 191 ISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFF 250
           +SES LK L+S N  I I +LT+IL+N +Y SR+YSV+LL S+ EVAD IQ+ NL  + F
Sbjct: 184 LSESGLKVLISKNAEI-IDSLTRILKNGNYESRAYSVLLLKSILEVADPIQLINLRQELF 243

Query: 251 IEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRL 310
           +EI+QIL+DQIS QA+KS+L+LL+ +    RNRVKAV A AV VLI+LLLD   S  KR+
Sbjct: 244 VEIVQILRDQISHQATKSSLRLLVNLCPWGRNRVKAVAANAVSVLINLLLD---SSEKRV 303

Query: 311 CEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQ 370
           CEM L++LD +C CA+GRAELL H AG+AVVSKKILR+S V SEKAV IL SI++FS + 
Sbjct: 304 CEMALMVLDLVCQCAEGRAELLGHGAGLAVVSKKILRVSQVASEKAVRILLSISKFSATN 363

Query: 371 SVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 426
           +V+QEM++IGVV KLC +LQ V  G+K KEKA+E+LKLH R W+NS C+P+ L S YP
Sbjct: 364 NVLQEMLQIGVVAKLCLVLQ-VDCGIKNKEKAREVLKLHARAWKNSPCIPANLLSFYP 416

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PUB22_ARATH3.1e-11750.46E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana GN=PUB22 PE=1 SV=1[more]
PUB23_ARATH4.2e-11449.64E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana GN=PUB23 PE=1 SV=1[more]
PUB24_ARATH8.0e-6535.96E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana GN=PUB24 PE=1 SV=1[more]
PUB25_ARATH8.9e-4832.78U-box domain-containing protein 25 OS=Arabidopsis thaliana GN=PUB25 PE=2 SV=1[more]
PUB26_ARATH1.3e-4632.16U-box domain-containing protein 26 OS=Arabidopsis thaliana GN=PUB26 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1F5_CUCSA6.8e-22092.97Uncharacterized protein OS=Cucumis sativus GN=Csa_4G194780 PE=4 SV=1[more]
W9SK34_9ROSA2.0e-13458.49E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_016918 PE=4 SV=1[more]
A0A0D2QAB1_GOSRA9.7e-13458.55Uncharacterized protein OS=Gossypium raimondii GN=B456_006G148900 PE=4 SV=1[more]
A0A067KIQ1_JATCU4.5e-13159.81Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08750 PE=4 SV=1[more]
A0A061EVP3_THECC5.9e-13156.83Plant U-box 23 OS=Theobroma cacao GN=TCM_022966 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G52450.11.8e-11850.46 plant U-box 22[more]
AT2G35930.12.4e-11549.64 plant U-box 23[more]
AT3G11840.12.7e-6635.89 plant U-box 24[more]
AT3G19380.15.0e-4932.78 plant U-box 25[more]
AT1G49780.17.3e-4832.16 plant U-box 26[more]
Match NameE-valueIdentityDescription
gi|659082653|ref|XP_008441961.1|3.9e-237100.00PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo][more]
gi|778692549|ref|XP_011653475.1|9.7e-22092.97PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis sativus][more]
gi|703139476|ref|XP_010106989.1|2.8e-13458.49E3 ubiquitin-protein ligase [Morus notabilis][more]
gi|823174256|ref|XP_012485721.1|1.4e-13358.55PREDICTED: E3 ubiquitin-protein ligase PUB23 [Gossypium raimondii][more]
gi|802611106|ref|XP_012074309.1|6.5e-13159.81PREDICTED: E3 ubiquitin-protein ligase PUB22 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003613Ubox_domain
IPR011989ARM-like
IPR013083Znf_RING/FYVE/PHD
IPR016024ARM-type_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
GO:0005488binding
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0005488 binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU51828melon EST collection version 4.0transcribed_cluster
MU65817melon EST collection version 4.0transcribed_cluster
MU67149melon EST collection version 4.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C008784T1MELO3C008784T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU65817MU65817transcribed_cluster
MU51828MU51828transcribed_cluster
MU67149MU67149transcribed_cluster


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainPFAMPF04564U-boxcoord: 14..83
score: 8.4
IPR003613U box domainSMARTSM00504Ubox_2coord: 16..80
score: 5.5
IPR003613U box domainPROFILEPS51698U_BOXcoord: 12..87
score: 41
IPR011989Armadillo-like helicalGENE3DG3DSA:1.25.10.10coord: 179..403
score: 3.
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 12..84
score: 1.1
IPR016024Armadillo-type foldunknownSSF48371ARM repeatcoord: 104..387
score: 9.75
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 1..425
score: 2.0E
NoneNo IPR availablePANTHERPTHR22849:SF38E3 UBIQUITIN-PROTEIN LIGASE PUB22-RELATEDcoord: 1..425
score: 2.0E
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 8..87
score: 7.57