Carg16772 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16772
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Mediator of RNA polymerase II transcription subunit 31
Location	Cucurbita_argyrosperma_scaffold_087 : 419008 .. 421554 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCAATCGCTTGCGGCTCTGGGGTTGAATGTAGCCATTGTTGAGAGCTCTGAACTTCGATCGCACGAGCTTTAAAGTTCAACTTGTCGGAATCAATGGCTTCTAATCATGAATTAAACGAAGAAGCAGATGATAATCCATCATCGTAAGTGTGTTTCTTGGTTCCTTCTCGTGTTCTCTCATTTCCCTTCCTCTTTTATTTCCTTTTAGCTAATTCTTCCTTCGTTTTTGCTATAGCTTCAATTCGAATTTCCTGTTCTGTTGAGCTGCTTGTGAGTTAATCAATTTTCCTTTCCTGTTTGGCGAATCATTACCAGGCCTACGAATGTCTACAAAGATCCGGATGACGGGCGGCAGCGGTTCTTGCTCGAATTGGAATTTGTTCAATGTCTTGCCAATCCAACCTACATTCATTGTACGTTTTTGCATAATTTAGGGTTTTTATATATGGAATGTTAGGACGATTTCTAATGCTCGAAGTGAGGAAAAAGCTATTGGATGTTCCAAAACAATAGAAATTTTCGAGTTTCTTCTTGTTTTAGTTAGAGAATTACTTCCAAACTCTTTCAGTTGAATTTAGTTTCAATTTGGAATTCTAATGAGCTAAATAGACAATGAAAAGATTTAGGTATTCAGAACCGAAGTGTGAGATCCCATATCAGTTGGAGAGAGGAACGAAACATTCTCTAAGAGGGTGTGGAAATCTCTCATAGTACACGCGTTTTAAAACCTTGAGAGGAACCTTGAAAGGGAAAGTCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGAGTTGTTACAAATGGTATCAGAGGCAGACACCGAATGGTATGCCAGCGAGGACGTTGGGCCCCAAAGAGGGTGGATTGTGAGATCCCACATTGAGTGGAGAGGGGAATGAAACATTCCTTACGAGGGTGTGGAAACCTCTTCCTAGTAGATGCGTTTTAGAACCTTGAGAGGAACCTTGAAAGGGAAAGTCTAAAGAGGACAATATCTGCTAGCGGTGGGCTTGAGTTGTTACAAATGATATCAGAGACAGACACCGAACGGTATGCTAGCGAGGACGTTGGGCCCCAAAGGGGGTGGATTGTGAGATCCCACATTGGTTGGAAAGGGAAATGAAACATTCCTTAGGAGGGTGTGGAAACCTCTTCCTAGTAGACGCGTTTTAAAACCTTGAGGGAAAGTTCAAAAGAGAAAGTAAAAAAAAGGACAATATTGGCTAGCCCTAAACTGGCATCTCCATGCCTTATCTTTTAAGCTTGACTGCACCTGTAACTTACAGTTTTTACTACAAAATTAATCTGTTATGAGCCTTCTCAACTCACGAGATCAGTGCTCGTCTGCTTGATCTTTCTTTCGACGTTATGTTATGGTCTCGTACGACTTAAGTAGGTTTCTGGACCTGTCGAACTTCTTGTGGTTGCCATCTTCACGAACTCACTAGGATATGCTTCTTAACGAACGATGCTCGTTCAAATTGGTATCGAAATGTTGAATCAAATGCTTTTTATGCTCCGAGTTCTATGGCTGATCACCGAGTTTTTATACAGATTTGGCTCAGAATCGTTACCTCGAGGACGAAGCTTTTATTGGTTACTTGAAGTACCTTCAATATTGGCAACAACCGGAGTATATTAAGTACATAATGTGAGTAAGATCTTTCCTTCATGTTCTTCATAAGCTTTTCCTGAACTGAAGAATTAGAACTGAATGAGATTTGGACTTCATTGTTGTTTCTTCTAGGTATCCTCATTGTCTTTTTTTCCTTGAACTTCTACAAAATGCAAACTTCCGAAATGCAATGGCTCATCCTGCCAACAAGGTTAACTTTTACATTTCATGTTCGTCGTGACTGATCGACGTCCATCGATTCCCCGAGAGAAGATAATTTTTCGCTTTCCGAGTGGTTTGCTCTTTATGTGCTGAAATTGCCCTTTTCCTGTTTCGTAGTATTTTGACCGTTGCGTCGTTTGGAATTTCTTAGGAACTGACACACAGACAGCAATTTTACTTCTGGAAGAACTATCGGAACAATCGTTTGAAACACATTTTACCTCGACCCCTACTCGAACCTGCAGCTCAACCACCTCCGGTTTCTGGTCCTCCTCAGGCAGCTGTGTCGGCCCCAGCCTCTGCTATGGCAGCTTCTCCTGCTGGTACCCCCCTTTCTCCAATGCAGTATGGTGTTCCCCCTGGTTCCGGGATTTCAAAGAACGATATGAAGAATGCAGGAATCGATCGACGAAAGAGAAAACACGTAATTTATCGCATTATCAATGTGCTCTACTAGTAGGTTAGAAAACACCCTTTTCCCTTTTCCCTGCAAATTCTCAGTTACTTGTTCTGTGTTTTGACAGGAAAGAAGTATGCCGTAGTTGGTGGATGCTTGATAGCAGCTCATGAGGTAATCTTTCATAAACTTAAAACTTTCCAAGAGCTTCCCATCACTTGGGATCATGCTTACATGTCTAAAGATCATTCTTTTTGGCTTAATTCAAGTAAATGTTATAATTATTGTTTTGGCGATTTTTCGTTCGGATC

mRNA sequence

TTTCAATCGCTTGCGGCTCTGGGGTTGAATGTAGCCATTGTTGAGAGCTCTGAACTTCGATCGCACGAGCTTTAAAGTTCAACTTGTCGGAATCAATGGCTTCTAATCATGAATTAAACGAAGAAGCAGATGATAATCCATCATCGCCTACGAATGTCTACAAAGATCCGGATGACGGGCGGCAGCGGTTCTTGCTCGAATTGGAATTTGTTCAATGTCTTGCCAATCCAACCTACATTCATTATTTGGCTCAGAATCGTTACCTCGAGGACGAAGCTTTTATTGGTTACTTGAAGTACCTTCAATATTGGCAACAACCGGAGTATATTAAGTACATAATGTATCCTCATTGTCTTTTTTTCCTTGAACTTCTACAAAATGCAAACTTCCGAAATGCAATGGCTCATCCTGCCAACAAGGAACTGACACACAGACAGCAATTTTACTTCTGGAAGAACTATCGGAACAATCGTTTGAAACACATTTTACCTCGACCCCTACTCGAACCTGCAGCTCAACCACCTCCGGTTTCTGGTCCTCCTCAGGCAGCTGTGTCGGCCCCAGCCTCTGCTATGGCAGCTTCTCCTGCTGGTACCCCCCTTTCTCCAATGCAGTATGGTGTTCCCCCTGGTTCCGGGATTTCAAAGAACGATATGAAGAATGCAGGAATCGATCGACGAAAGAGAAAACACGAAAGAAGTATGCCGTAGTTGGTGGATGCTTGATAGCAGCTCATGAGGTAATCTTTCATAAACTTAAAACTTTCCAAGAGCTTCCCATCACTTGGGATCATGCTTACATGTCTAAAGATCATTCTTTTTGGCTTAATTCAAGTAAATGTTATAATTATTGTTTTGGCGATTTTTCGTTCGGATC

Coding sequence (CDS)

ATGGCTTCTAATCATGAATTAAACGAAGAAGCAGATGATAATCCATCATCGCCTACGAATGTCTACAAAGATCCGGATGACGGGCGGCAGCGGTTCTTGCTCGAATTGGAATTTGTTCAATGTCTTGCCAATCCAACCTACATTCATTATTTGGCTCAGAATCGTTACCTCGAGGACGAAGCTTTTATTGGTTACTTGAAGTACCTTCAATATTGGCAACAACCGGAGTATATTAAGTACATAATGTATCCTCATTGTCTTTTTTTCCTTGAACTTCTACAAAATGCAAACTTCCGAAATGCAATGGCTCATCCTGCCAACAAGGAACTGACACACAGACAGCAATTTTACTTCTGGAAGAACTATCGGAACAATCGTTTGAAACACATTTTACCTCGACCCCTACTCGAACCTGCAGCTCAACCACCTCCGGTTTCTGGTCCTCCTCAGGCAGCTGTGTCGGCCCCAGCCTCTGCTATGGCAGCTTCTCCTGCTGGTACCCCCCTTTCTCCAATGCAGTATGGTGTTCCCCCTGGTTCCGGGATTTCAAAGAACGATATGAAGAATGCAGGAATCGATCGACGAAAGAGAAAACACGAAAGAAGTATGCCGTAG

Protein sequence

MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKYLQYWQQPEYIKYIMYPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWKNYRNNRLKHILPRPLLEPAAQPPPVSGPPQAAVSAPASAMAASPAGTPLSPMQYGVPPGSGISKNDMKNAGIDRRKRKHERSMP

Homology

[Close]

BLAST of Carg16772 vs. NCBI nr
Match: XP_022922582.1 (mediator of RNA polymerase II transcription subunit 31-like [Cucurbita moschata])

HSP 1 Score: 305.4 bits (781), Expect = 1.4e-79
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK
Sbjct: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGS 180
           NYRNNRLKHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGS
Sbjct: 121 NYRNNRLKHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGS 180

Query: 181 GISKNDMKNAGIDRRKRKHERSM 204
           GISKNDMKNAGIDRRKRKHERSM
Sbjct: 181 GISKNDMKNAGIDRRKRKHERSM 203

[Close]

BLAST of Carg16772 vs. NCBI nr
Match: XP_023551913.1 (mediator of RNA polymerase II transcription subunit 31-like [Cucurbita pepo subsp. pepo] >XP_023551914.1 mediator of RNA polymerase II transcription subunit 31-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.0 bits (772), Expect = 1.6e-78
Identity = 192/204 (94.12%), Postives = 192/204 (94.12%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 5   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 64

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AF XXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK
Sbjct: 65  AFXXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 124

Query: 121 NYRNNRLKHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGS 180
           NYRNNRLKHILPRPLLEPAA          XXXXXXXXXXXXXXXXX LSPMQYGVPPGS
Sbjct: 125 NYRNNRLKHILPRPLLEPAA---------QXXXXXXXXXXXXXXXXXPLSPMQYGVPPGS 184

Query: 181 GISKNDMKNAGIDRRKRKHERSMP 205
           GISKNDMKNAGIDRRKRKHERSMP
Sbjct: 185 GISKNDMKNAGIDRRKRKHERSMP 199

[Close]

BLAST of Carg16772 vs. NCBI nr
Match: XP_022984406.1 (mediator of RNA polymerase II transcription subunit 31-like [Cucurbita maxima])

HSP 1 Score: 280.8 bits (717), Expect = 3.7e-72
Identity = 192/204 (94.12%), Postives = 192/204 (94.12%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK
Sbjct: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGS 180
           NYRNNRLKH           XXXXXXXXXXXXXXXXXXXXXXXXXXXX SPMQYGVPPGS
Sbjct: 121 NYRNNRLKHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSPMQYGVPPGS 180

Query: 181 GISKNDMKNAGIDRRKRKHERSMP 205
           GISKNDMKNAGIDRRKRKHERSMP
Sbjct: 181 GISKNDMKNAGIDRRKRKHERSMP 204

[Close]

BLAST of Carg16772 vs. NCBI nr
Match: XP_004146891.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis sativus] >KGN53186.1 hypothetical protein Csa_4G025160 [Cucumis sativus])

HSP 1 Score: 274.2 bits (700), Expect = 3.5e-70
Identity = 169/205 (82.44%), Postives = 173/205 (84.39%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNH L EE  DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFI                    PHCLFFLELLQN+NFRNAMAHP NKEL HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLLEPAA--XXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPP 180
           NYRNNRLKHILPRPL EPAA  XXXXXXXXXXXXXXXXXXXXXXXXXXXXLSP+QYG+PP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPIQYGIPP 180

Query: 181 GSGISKNDMKNAGIDRRKRKHERSM 204
           G G+ KNDMK AGIDRRKRKHERSM
Sbjct: 181 GPGLPKNDMKGAGIDRRKRKHERSM 205

[Close]

BLAST of Carg16772 vs. NCBI nr
Match: XP_022942362.1 (mediator of RNA polymerase II transcription subunit 31-like [Cucurbita moschata])

HSP 1 Score: 269.2 bits (687), Expect = 1.1e-68
Identity = 187/205 (91.22%), Postives = 192/205 (93.66%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASN+ LNEEA D+PSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNYGLNEEAADSPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFR+AMAHPANKEL HRQQFYFWK
Sbjct: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRSAMAHPANKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPR--PLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPP 180
           NYRNNRLKHILPR  P      XXXXXXXXXXXXXXXXXXXXXXXXXXX LSPMQYG+PP
Sbjct: 121 NYRNNRLKHILPRSFPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALSPMQYGIPP 180

Query: 181 GSGISKNDMKNAGIDRRKRKHERSM 204
           GSG+SKNDMKNA IDRRKRKHERSM
Sbjct: 181 GSGLSKNDMKNAAIDRRKRKHERSM 205

[Close]

BLAST of Carg16772 vs. TAIR10
Match: AT5G19910.2 (SOH1 family protein)

HSP 1 Score: 145.2 bits (365), Expect = 4.4e-35
Identity = 76/119 (63.87%), Postives = 83/119 (69.75%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MAS  E+ ++A + PS P N YKDPD GRQRFLLELEF+QCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFW 120
           AFI    XXXX            PHCL+FLELLQN NFR AMAHPANK       + FW
Sbjct: 61  AFIGYLKXXXXWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKWFKMLGYWCFW 119

[Close]

BLAST of Carg16772 vs. Swiss-Prot
Match: sp|Q8VYB1|MED31_ARATH (Mediator of RNA polymerase II transcription subunit 31 OS=Arabidopsis thaliana OX=3702 GN=MED31 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 8.2e-47
Identity = 135/201 (67.16%), Postives = 145/201 (72.14%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MAS  E+ ++A + PS P N YKDPD GRQRFLLELEF+QCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFI      XX            PHCL+FLELLQN NFR AMAHPANKEL HRQQFY+WK
Sbjct: 61  AFIGYLKYLXXWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKELAHRQQFYYWK 120

Query: 121 NYRNNRLKHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGS 180
           NYRNNRLKH           XXXXXXXXXXXXXXXXXXXXXXXXXXXX   MQY     +
Sbjct: 121 NYRNNRLKH----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMQY----NN 180

Query: 181 GISKNDMKN---AGIDRRKRK 199
            +SKND +N    GIDRRKRK
Sbjct: 181 MLSKNDTRNMGATGIDRRKRK 193

[Close]

BLAST of Carg16772 vs. Swiss-Prot
Match: sp|Q6DH26|MED31_DANRE (Mediator of RNA polymerase II transcription subunit 31 OS=Danio rerio OX=7955 GN=med31 PE=2 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-14
Identity = 60/108 (55.56%), Postives = 75/108 (69.44%), Query Frame = 0


Query: 21  VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIXXXXXXXXXXXXXXXXX 80
           V +  +  RQRF LELEFVQCLANP Y+++LAQ  YL ++ F+XXXXXXXXXXXXXXXXX
Sbjct: 4   VMETDEQARQRFQLELEFVQCLANPNYLNFLAQRGYLREKPFVXXXXXXXXXXXXXXXXX 63

Query: 81  XXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWKNYRNNRLK 129
           XXXPHCL  LELLQ  +FR  + +    +    QQ   W++Y   R +
Sbjct: 64  XXXPHCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRTR 111

[Close]

BLAST of Carg16772 vs. Swiss-Prot
Match: sp|Q9USH1|MED31_SCHPO (Mediator of RNA polymerase II transcription subunit 31 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=med31 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.2e-11
Identity = 42/97 (43.30%), Postives = 58/97 (59.79%), Query Frame = 0


Query: 23  KDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIXXXXXXXXXXXXXXXXXXX 82
           K PDD + RF +ELEFVQ L+NP Y+++LAQ++Y EDEAF+   XXXXX           
Sbjct: 9   KVPDD-KSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLXXXXXWREPEYVKFII 68

Query: 83  XPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFW 120
            P CL  L LL+N  FRN ++     +  + + +Y W
Sbjct: 69  YPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEW 104

[Close]

BLAST of Carg16772 vs. Swiss-Prot
Match: sp|A0JNN3|MED31_BOVIN (Mediator of RNA polymerase II transcription subunit 31 OS=Bos taurus OX=9913 GN=MED31 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.5e-11
Identity = 57/105 (54.29%), Postives = 70/105 (66.67%), Query Frame = 0


Query: 24  DPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIXXXXXXXXXXXXXXXXXXXX 83
           D    R RF LELEFVQCLANP Y+++LAQ  Y +D+A  XXXXXXXXXXXXXXXXXXXX
Sbjct: 10  DDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAXXXXXXXXXXXXXXXXXXXXXX 69

Query: 84  PHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWKNYRNNRLK 129
             CL  LELLQ  +FR  + +    +    QQ   W++Y   R++
Sbjct: 70  XXCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMR 114

[Close]

BLAST of Carg16772 vs. Swiss-Prot
Match: sp|Q9CXU1|MED31_MOUSE (Mediator of RNA polymerase II transcription subunit 31 OS=Mus musculus OX=10090 GN=Med31 PE=1 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 7.2e-11
Identity = 57/105 (54.29%), Postives = 70/105 (66.67%), Query Frame = 0


Query: 24  DPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIXXXXXXXXXXXXXXXXXXXX 83
           D    R RF LELEFVQCLANP Y+++LAQ  Y +D+A  XXXXXXXXXXXXXXXXXXXX
Sbjct: 10  DDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAXXXXXXXXXXXXXXXXXXXXXX 69

Query: 84  PHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWKNYRNNRLK 129
             CL  LELLQ  +FR  + +    +    QQ   W++Y   R++
Sbjct: 70  XXCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRVR 114

[Close]

BLAST of Carg16772 vs. TrEMBL
Match: tr|A0A0A0KZ88|A0A0A0KZ88_CUCSA (Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis sativus OX=3659 GN=Csa_4G025160 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.3e-70
Identity = 169/205 (82.44%), Postives = 173/205 (84.39%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNH L EE  DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFI                    PHCLFFLELLQN+NFRNAMAHP NKEL HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLLEPAA--XXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPP 180
           NYRNNRLKHILPRPL EPAA  XXXXXXXXXXXXXXXXXXXXXXXXXXXXLSP+QYG+PP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPIQYGIPP 180

Query: 181 GSGISKNDMKNAGIDRRKRKHERSM 204
           G G+ KNDMK AGIDRRKRKHERSM
Sbjct: 181 GPGLPKNDMKGAGIDRRKRKHERSM 205

[Close]

BLAST of Carg16772 vs. TrEMBL
Match: tr|A0A1S3BY12|A0A1S3BY12_CUCME (Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis melo OX=3656 GN=LOC103494449 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 7.6e-66
Identity = 162/205 (79.02%), Postives = 165/205 (80.49%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNH LNEEA DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHGLNEEAADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFI                    PHCLFFLELLQN+NFRNAMAHP NKEL HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHI--LPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPP 180
           NYRNNRLKHI            XXXXXXXXXXXXXXXXXXXXXXXXXXX LSPMQYG+PP
Sbjct: 121 NYRNNRLKHIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALSPMQYGIPP 180

Query: 181 GSGISKNDMKNAGIDRRKRKHERSM 204
           G G+ KNDMK AGIDRRKRKHERSM
Sbjct: 181 GPGLPKNDMKGAGIDRRKRKHERSM 205

[Close]

BLAST of Carg16772 vs. TrEMBL
Match: tr|V4TKG6|V4TKG6_9ROSI (Mediator of RNA polymerase II transcription subunit 31 OS=Citrus clementina OX=85681 GN=CICLE_v10032791mg PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.8e-60
Identity = 143/193 (74.09%), Postives = 151/193 (78.24%), Query Frame = 0


Query: 8   NEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIXXXX 67
           NEEA D PSSP  VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDEAFI    
Sbjct: 7   NEEASDAPSSPKKVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLK 66

Query: 68  XXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWKNYRNNRL 127
                           PHCL+FLELLQNANFRNAMAHPANKEL HRQQF+FWKNYRNNRL
Sbjct: 67  YLQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPANKELAHRQQFFFWKNYRNNRL 126

Query: 128 KHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGSGISKNDM 187
           KHILPRPL E   XXXXXXXXXXXXXXXXXXXX        LSPMQYG+PPGS + KNDM
Sbjct: 127 KHILPRPLPE-XXXXXXXXXXXXXXXXXXXXXXVTAAPGPALSPMQYGIPPGSALMKNDM 186

Query: 188 KNAGIDRRKRKHE 201
           +++ IDRRKRK +
Sbjct: 187 RSSSIDRRKRKKD 198

[Close]

BLAST of Carg16772 vs. TrEMBL
Match: tr|A0A2U1M1G9|A0A2U1M1G9_ARTAN (SOH1 family protein OS=Artemisia annua OX=35608 GN=CTI12_AA430100 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.5e-58
Identity = 137/200 (68.50%), Postives = 149/200 (74.50%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MAS  E+ +++ + PSSP  VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASGQEI-DDSSNTPSSPKKVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFI                    PHCL+FLELLQNA+FR AMAHPANKELTHRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNASFRTAMAHPANKELTHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLLEPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSPMQYGVPPGS 180
           NYRNNRLKHILPRPL EP        XXXXXXXXXXXXXXX       LSPMQYGVPPG 
Sbjct: 121 NYRNNRLKHILPRPLPEPTPALPSNAXXXXXXXXXXXXXXXVSAPSPVLSPMQYGVPPGP 180

Query: 181 GISKNDMKNAGIDRRKRKHE 201
            + KND + +G+DRRKRK +
Sbjct: 181 PLPKNDPR-SGLDRRKRKKD 198

[Close]

BLAST of Carg16772 vs. TrEMBL
Match: tr|A0A0D2N5R9|A0A0D2N5R9_GOSRA (Mediator of RNA polymerase II transcription subunit 31 OS=Gossypium raimondii OX=29730 GN=B456_001G052400 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.6e-58
Identity = 140/208 (67.31%), Postives = 153/208 (73.56%), Query Frame = 0


Query: 1   MASNHELNEEADDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MAS  E ++ A D PSSP NVYKDPDDGRQRFLLELEF+QCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASTKE-SDNASDTPSSPKNVYKDPDDGRQRFLLELEFLQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIXXXXXXXXXXXXXXXXXXXXPHCLFFLELLQNANFRNAMAHPANKELTHRQQFYFWK 120
           AFI    XXXXX           PHCL+FLELLQNANFRNAMAHPANKE+ HRQQF+FWK
Sbjct: 61  AFIGYLKXXXXXQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPANKEVAHRQQFFFWK 120

Query: 121 NYRNNRLKHILPRPLLE----PAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXL-SPMQYG 180
           NYRNNRLK ILP+P  E    PA XXXXXXXXXX                    SPM YG
Sbjct: 121 NYRNNRLKFILPKPPPEEVPTPAPXXXXXXXXXXSLPASNIAMTTAPPAPASTHSPMPYG 180

Query: 181 VPPGSGISKNDMKNAGIDRRKRKHERSM 204
           +P GS ++KNDM+N+GIDRRKRKHERS+
Sbjct: 181 LPSGSALAKNDMRNSGIDRRKRKHERSL 207

The following BLAST results are available for this feature:

BLAST of Carg16772 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922582.1	1.4e-79	100.00	mediator of RNA polymerase II transcription subunit 31-like [Cucurbita moschata]	[more]
XP_023551913.1	1.6e-78	94.12	mediator of RNA polymerase II transcription subunit 31-like [Cucurbita pepo subs...	[more]
XP_022984406.1	3.7e-72	94.12	mediator of RNA polymerase II transcription subunit 31-like [Cucurbita maxima]	[more]
XP_004146891.1	3.5e-70	82.44	PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis sativ...	[more]
XP_022942362.1	1.1e-68	91.22	mediator of RNA polymerase II transcription subunit 31-like [Cucurbita moschata]	[more]

BLAST of Carg16772 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19910.2	4.4e-35	63.87	SOH1 family protein	[more]

BLAST of Carg16772 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8VYB1\|MED31_ARATH	8.2e-47	67.16	Mediator of RNA polymerase II transcription subunit 31 OS=Arabidopsis thaliana O...	[more]
sp\|Q6DH26\|MED31_DANRE	2.4e-14	55.56	Mediator of RNA polymerase II transcription subunit 31 OS=Danio rerio OX=7955 GN...	[more]
sp\|Q9USH1\|MED31_SCHPO	4.2e-11	43.30	Mediator of RNA polymerase II transcription subunit 31 OS=Schizosaccharomyces po...	[more]
sp\|A0JNN3\|MED31_BOVIN	5.5e-11	54.29	Mediator of RNA polymerase II transcription subunit 31 OS=Bos taurus OX=9913 GN=...	[more]
sp\|Q9CXU1\|MED31_MOUSE	7.2e-11	54.29	Mediator of RNA polymerase II transcription subunit 31 OS=Mus musculus OX=10090 ...	[more]

BLAST of Carg16772 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KZ88\|A0A0A0KZ88_CUCSA	2.3e-70	82.44	Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis sativus OX=365...	[more]
tr\|A0A1S3BY12\|A0A1S3BY12_CUCME	7.6e-66	79.02	Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis melo OX=3656 G...	[more]
tr\|V4TKG6\|V4TKG6_9ROSI	4.8e-60	74.09	Mediator of RNA polymerase II transcription subunit 31 OS=Citrus clementina OX=8...	[more]
tr\|A0A2U1M1G9\|A0A2U1M1G9_ARTAN	1.5e-58	68.50	SOH1 family protein OS=Artemisia annua OX=35608 GN=CTI12_AA430100 PE=4 SV=1	[more]
tr\|A0A0D2N5R9\|A0A0D2N5R9_GOSRA	2.6e-58	67.31	Mediator of RNA polymerase II transcription subunit 31 OS=Gossypium raimondii OX...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003712	transcription cofactor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **Cellular Component**
Term	Definition
GO:0016592	mediator complex

Vocabulary: **INTERPRO**
Term	Definition
IPR008831	Mediator_Med31
IPR038089	Med31_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003712	transcription cofactor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16772-RA	Carg16772-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038089	Mediator complex, subunit Med31 domain superfamily	GENE3D	G3DSA:1.10.10.1340		coord: 23..115 e-value: 1.1E-33 score: 116.6
IPR008831	Mediator complex, subunit Med31	PFAM	PF05669	Med31	coord: 30..122 e-value: 1.6E-35 score: 120.8
IPR008831	Mediator complex, subunit Med31	PANTHER	PTHR13186	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT SOH1	coord: 8..143
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..204
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 188..204

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg16772	Cla010693	Watermelon (97103) v1	carwmB0873
Carg16772	Csa4G025160	Cucumber (Chinese Long) v2	carcuB0886
Carg16772	MELO3C017626	Melon (DHL92) v3.5.1	carmeB0862
Carg16772	ClCG07G017200	Watermelon (Charleston Gray)	carwcgB0848
Carg16772	CSPI04G04310	Wild cucumber (PI 183967)	carcpiB0906
Carg16772	Cucsa.093930	Cucumber (Gy14) v1	carcgyB0992
Carg16772	CmaCh16G002300	Cucurbita maxima (Rimu)	carcmaB1206
Carg16772	CmaCh04G003740	Cucurbita maxima (Rimu)	carcmaB1209
Carg16772	CmoCh04G003950	Cucurbita moschata (Rifu)	carcmoB1177
Carg16772	Lsi07G001380	Bottle gourd (USVL1VR-Ls)	carlsiB792
Carg16772	Cp4.1LG01g03360	Cucurbita pepo (Zucchini)	carcpeB1216
Carg16772	CsGy4G004220	Cucumber (Gy14) v2	carcgybB0860
Carg16772	MELO3C017626.2	Melon (DHL92) v3.6.1	carmedB0872
Carg16772	CsaV3_4G004400	Cucumber (Chinese Long) v3	carcucB0910
Carg16772	Cla97C07G144220	Watermelon (97103) v2	carwmbB0911
Carg16772	Bhi01G001986	Wax gourd	carwgoB1020

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg16772	Carg08048	Silver-seed gourd	carcarB406

The following block(s) are covering this gene:


Gene	Organism	Block
Carg16772	Watermelon (97103) v2	carwmbB0908
Carg16772	Cucurbita moschata (Rifu)	carcmoB1175
Carg16772	Cucurbita pepo (Zucchini)	carcpeB1215