CSPI04G04310 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G04310
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Mediator of RNA polymerase II transcription subunit 31
Location	Chr4 : 2809673 .. 2812829 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAAGTATTTTGAGGTTTTTATAGCATTGGTAGCGCGGGCATATTTCATTGTTCTACGTTGGGTCATCGATTTCAATCGCTTGCTGCTCTAAGTTGAATGTAGCAATTGTTGAGAGCTTTGAAGGGTATCTCCATATCGCTCCACTACGAATCTCGCAACTTCTTCTTAATTTTTGGGTTCAATTTGAACTTCGACTGGCAGCCAGATTCAATGGCGTCCAATCATGGATTAAAAGAAGAAGAAGCTGATAACCCATCATCGTAAGTTTGTTTCTCTTTTTCATTCTCCTGTTCTATCATTTTCCCTCCTCCGTCTCTCTTTACCCAATTCTTCTTTCATTTTGTATATCGCTTCAATTCTTCATTTTTGTTCCGTTGCTGAGGGATTGGTTTATTGGGCTCATTGTGAATTGATCAATTTTCCCTTTCTGTTTGGTTAATTATTACCAGACCTACCAATGTTTACAAAGATCCCGATGACGGACGGCAGCGGTTCTTGCTCGAATTGGAGTTTGTTCAATGCCTTGCCAATCCTACCTACATTCATTGTAAGTTTTTTGTAAAATTTAGGGTTTCTTTATAGGGGGTTAGGCTTATTTCTTATCCTTGAAACTTCAGCTTGAAGGGTGGGCAAAGCTATTGGATGTTCCAAAATGAGTTTCGAAAATTCAGCGTTTATCCTAAGATTTGCTTTCGAACCTTTTCAGTTGAATTTAGTTTCAATTTGGATTTCTTATGAGCTAACTATACATGAAAGTGTGCTTTACATGAAAAGCAACCAAGACGAAAGTAAATTCTACGTATTTCTTCTAATGATAAAGTGGATTGAACATATGTGTATAATAAATGTTAATGTAAAATTAAATAATCACTTGGAGTGGGTTCAATCTACGACGACCATCTACCAATGATTTAATACCGTATGAGTTTCCTTGATAACAAATATCAGTATAGTATTGTTATAGTTATTCCATTCGATTAGTTGAGGTCTAACAAGCTAGCTTGAACATCCAGGATATGAAAGAAAAAGAACTGTTGGCTTGAATTTTTATCATATAATATCTCACGTGAACCATCTGTTTTATTTGAATTTTGAACATGATTTTCTCAGTTGCATTTCCTGTGAAGTGATTTTTTATTGACCAATGTGAAGAAGGAAGTCACAATGAAAGATCAGATTTTGACATCTAGAACCAAAATTCATTCAATTGCATCTTCTTGAGTTAGAGTAGTGCCTAGATGAAACTTTTTCTTTTATGCTGGTTGCCTGACTACTACTGGGGCAGCAACCTACTGTTTCTACTACAAAATCAACTTATAATTTACCAGAGATCAGTGCTCGTCTGATCTTTCTGCCTGTTGAGAAAAACTGGCGTTGTACAATTTTTACATATAGTGAATTTCCTAGAATTTTATCCTTTTGATGATATGCTACAGTGTCTTACTGAAGTACATTTCTGAAGTTGGCACTTACATATTTGGTGATAGTCATCTTCACGTACACATTTCTTCTTAATGAATGATGTATTTGCAAATTGATATTGAAATGTTCGAACAAATGCTTTTCTCAGTTTTGGTTGCTGATCATCTAGTTTTATACAGATCTGGCGCAGAATCGTTACCTCGAGGATGAAGCTTTTATTGGTTACTTGAAGTACCTTCAATATTGGCAACGGCCAGAGTATATCAAGTTTATAATGTGAGTCAGTTCTTTCCCTGAACTGATGAATGAGAACTGGTTGTACTTTTGGTATTGACTTCAATGTTGTTTCTAGGTACCCTCATTGTCTTTTTTTTCTTGAACTTCTACAAAATTCAAATTTCCGGAATGCAATGGCTCATCCTGGCAACAAGGTTAGCTTCTGTTATTTTTTGTTCAACTTCTCTTTCGAGATCGGAGAATAAATATTTAGTTGGATAGTGAATTTTTTCTGTGATCACGACTATGCACATACATCTCTGATAAATGCTCAGTAGACTTATATATTTTCATTTCAGTTTATAGTGAGATGAAAAAATCTAAACTGTCTCCCATGTCCATCGATTTCAAAAGAGATATTTGTTGCTTCTTTCCAAATGGTTTGCTCATATGCGGATATGTGATATTTCCCCTTTTCCTTTTTCTTAGTATTTTAACACAGTTGCATCATTTGGAATTTCTTAGGAATTGGCACACAGACAGCAATTTTACTTTTGGAAGAACTATAGGAACAATCGATTGAAACACATTTTGCCTCGACCTCTTCCCGAACCTGCAGCATTACCACCCCCAGTCACTGCTCCACCTCAAGCAGCTGTACCGGCTCCAACCTCAGCCCCCAATATGGCAGCTTCACCTGCTGCACCTGCACTTTCTCCCATGCAGTATGGTATTCCACCTGGTCCTGGTCTTCCAAAGAACGACATGAAGGGTGCAGGAATTGATCGACGAAAGAGAAAACATGTAATTTATCACATATAGTTTGTTTTCTTAAGTTATCAATGTACTCTACTTATTAGGTTAGAAGACACACCTCTTCCTTAAACCTTCAAATTTTCATTTACTTATTTTGTGTGTTTGACAGGAAAGAAGTATGACGTAGTTGGTTTCATTAGTCAAAACTAGGGTGGATGCTGGAGCAGCTCATGAGGTAAAATCTGCATACAGTGAAAATTAAAGTTCAAAGATGATGCCTTTCATAAGCTTTCCAAGAGTTATCCTTCACTTCAGGTCGTTCAAAATCTCTCTCTCGCATGTTCGTGAACACGAGCTACATGACATCCATGTTTATGGGTTCATGTAATATATCCTTTGATGAAGATCTGCATGACGTTGGGATAAAATATTGCTAAGAATCCTTAAAATTTTTGGTTTATCTATATGTTTGGCTGTTATAGATAATACATTGATAAGCAGTGTCTTTGACTATCTTTTTATCTCCATCTATATTAGACATTTTTTTTTTGTAGCAGCATGAAATCCTATAAGTAGGTATGGTGGGAGTGAGCGCTAACGTGAATATAGCAAAATATTATTGTTTACTAATGATGTATATTGATAGATTATTATCATCTATTGGTGATAGACACAAATAGATGTTTACTATGTTTATTTATCACGGTGACATTTTGTTATATTTGTAAATATTATTCGCTATATATATTATTGATTATGAT

mRNA sequence

ATGGCGTCCAATCATGGATTAAAAGAAGAAGAAGCTGATAACCCATCATCACCTACCAATGTTTACAAAGATCCCGATGACGGACGGCAGCGGTTCTTGCTCGAATTGGAGTTTGTTCAATGCCTTGCCAATCCTACCTACATTCATTATCTGGCGCAGAATCGTTACCTCGAGGATGAAGCTTTTATTGGTTACTTGAAGTACCTTCAATATTGGCAACGGCCAGAGTATATCAAGTTTATAATGTACCCTCATTGTCTTTTTTTTCTTGAACTTCTACAAAATTCAAATTTCCGGAATGCAATGGCTCATCCTGGCAACAAGGAATTGGCACACAGACAGCAATTTTACTTTTGGAAGAACTATAGGAACAATCGATTGAAACACATTTTGCCTCGACCTCTTCCCGAACCTGCAGCATTACCACCCCCAGTCACTGCTCCACCTCAAGCAGCTGTACCGGCTCCAACCTCAGCCCCCAATATGGCAGCTTCACCTGCTGCACCTGCACTTTCTCCCATGCAGTATGGTATTCCACCTGGTCCTGGTCTTCCAAAGAACGACATGAAGGGTGCAGGAATTGATCGACGAAAGAGAAAACATGAAAGAAGTATGACGTAG

Coding sequence (CDS)

ATGGCGTCCAATCATGGATTAAAAGAAGAAGAAGCTGATAACCCATCATCACCTACCAATGTTTACAAAGATCCCGATGACGGACGGCAGCGGTTCTTGCTCGAATTGGAGTTTGTTCAATGCCTTGCCAATCCTACCTACATTCATTATCTGGCGCAGAATCGTTACCTCGAGGATGAAGCTTTTATTGGTTACTTGAAGTACCTTCAATATTGGCAACGGCCAGAGTATATCAAGTTTATAATGTACCCTCATTGTCTTTTTTTTCTTGAACTTCTACAAAATTCAAATTTCCGGAATGCAATGGCTCATCCTGGCAACAAGGAATTGGCACACAGACAGCAATTTTACTTTTGGAAGAACTATAGGAACAATCGATTGAAACACATTTTGCCTCGACCTCTTCCCGAACCTGCAGCATTACCACCCCCAGTCACTGCTCCACCTCAAGCAGCTGTACCGGCTCCAACCTCAGCCCCCAATATGGCAGCTTCACCTGCTGCACCTGCACTTTCTCCCATGCAGTATGGTATTCCACCTGGTCCTGGTCTTCCAAAGAACGACATGAAGGGTGCAGGAATTGATCGACGAAAGAGAAAACATGAAAGAAGTATGACGTAG

Homology

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BLAST of CSPI04G04310 vs. Swiss-Prot
Match: MED31_ARATH (Mediator of RNA polymerase II transcription subunit 31 OS=Arabidopsis thaliana GN=MED31 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 1.9e-72
Identity = 146/203 (71.92%), Postives = 158/203 (77.83%), Query Frame = 1


Query: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MAS   + ++ ++ PS P N YKDPD GRQRFLLELEF+QCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPHCL+FLELLQN NFR AMAHP NKELAHRQQFY+WK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKELAHRQQFYYWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEP    PPV AP  +  PAP+     A +  +PALSPMQY    
Sbjct: 121 NYRNNRLKHILPRPLPEPVPPQPPV-APSTSLPPAPS-----ATAALSPALSPMQY---- 180

Query: 181 GPGLPKND---MKGAGIDRRKRK 201
              L KND   M   GIDRRKRK
Sbjct: 181 NNMLSKNDTRNMGATGIDRRKRK 193

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BLAST of CSPI04G04310 vs. Swiss-Prot
Match: MED31_DICDI (Putative mediator of RNA polymerase II transcription subunit 31 OS=Dictyostelium discoideum GN=med31 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.3e-31
Identity = 57/97 (58.76%), Postives = 76/97 (78.35%), Query Frame = 1


Query: 31  RFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKYLQYWQRPEYIKFIMYPHCLFFL 90
           RF++ELEF+QCL+NP Y++YLAQNRY +D+AF+ YL YLQYW++PEY KFI+YP  L+FL
Sbjct: 55  RFIMELEFIQCLSNPRYLNYLAQNRYFQDKAFVNYLVYLQYWKKPEYAKFIVYPQSLYFL 114

Query: 91  ELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRL 128
           +LLQ   FR  + H  + +  H QQFY W+ YRNNR+
Sbjct: 115 DLLQEERFRQELNHSQSTDFIHEQQFYHWQYYRNNRM 151

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BLAST of CSPI04G04310 vs. Swiss-Prot
Match: MED31_DANRE (Mediator of RNA polymerase II transcription subunit 31 OS=Danio rerio GN=med31 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-25
Identity = 53/108 (49.07%), Postives = 70/108 (64.81%), Query Frame = 1


Query: 21  VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKYLQYWQRPEYIKF 80
           V +  +  RQRF LELEFVQCLANP Y+++LAQ  YL ++ F+ YLKYL YW+ PEY KF
Sbjct: 4   VMETDEQARQRFQLELEFVQCLANPNYLNFLAQRGYLREKPFVNYLKYLLYWKEPEYAKF 63

Query: 81  IMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 129
           + YPHCL  LELLQ  +FR  + +    +    QQ   W++Y   R +
Sbjct: 64  LKYPHCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRTR 111

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BLAST of CSPI04G04310 vs. Swiss-Prot
Match: MED31_BOVIN (Mediator of RNA polymerase II transcription subunit 31 OS=Bos taurus GN=MED31 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.3e-24
Identity = 51/105 (48.57%), Postives = 68/105 (64.76%), Query Frame = 1


Query: 24  DPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKYLQYWQRPEYIKFIMY 83
           D    R RF LELEFVQCLANP Y+++LAQ  Y +D+AF+ YLKYL YW+ PEY K++ Y
Sbjct: 10  DDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKEPEYAKYLKY 69

Query: 84  PHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 129
           P CL  LELLQ  +FR  + +    +    QQ   W++Y   R++
Sbjct: 70  PQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMR 114

[Close]

BLAST of CSPI04G04310 vs. Swiss-Prot
Match: MED31_MOUSE (Mediator of RNA polymerase II transcription subunit 31 OS=Mus musculus GN=Med31 PE=1 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 4.4e-24
Identity = 51/105 (48.57%), Postives = 68/105 (64.76%), Query Frame = 1


Query: 24  DPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKYLQYWQRPEYIKFIMY 83
           D    R RF LELEFVQCLANP Y+++LAQ  Y +D+AF+ YLKYL YW+ PEY K++ Y
Sbjct: 10  DDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKEPEYAKYLKY 69

Query: 84  PHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 129
           P CL  LELLQ  +FR  + +    +    QQ   W++Y   R++
Sbjct: 70  PQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRVR 114

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BLAST of CSPI04G04310 vs. TrEMBL
Match: A0A0A0KZ88_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025160 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 7.1e-114
Identity = 205/206 (99.51%), Postives = 206/206 (100.00%), Query Frame = 1


Query: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSP+QYGIPP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPIQYGIPP 180

Query: 181 GPGLPKNDMKGAGIDRRKRKHERSMT 207
           GPGLPKNDMKGAGIDRRKRKHERSMT
Sbjct: 181 GPGLPKNDMKGAGIDRRKRKHERSMT 206

[Close]

BLAST of CSPI04G04310 vs. TrEMBL
Match: V4TKG6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10032791mg PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.2e-81
Identity = 156/194 (80.41%), Postives = 166/194 (85.57%), Query Frame = 1


Query: 9   EEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKY 68
           EE +D PSSP  VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDEAFIGYLKY
Sbjct: 8   EEASDAPSSPKKVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKY 67

Query: 69  LQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 128
           LQYWQRPEYIKFIMYPHCL+FLELLQN+NFRNAMAHP NKELAHRQQF+FWKNYRNNRLK
Sbjct: 68  LQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPANKELAHRQQFFFWKNYRNNRLK 127

Query: 129 HILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPPGPGLPKND 188
           HILPRPLPEP+  PPP  APP    PAP     + A+P  PALSPMQYGIPPG  L KND
Sbjct: 128 HILPRPLPEPSEAPPPAAAPP--LPPAPPVLTPVTAAP-GPALSPMQYGIPPGSALMKND 187

Query: 189 MKGAGIDRRKRKHE 203
           M+ + IDRRKRK +
Sbjct: 188 MRSSSIDRRKRKKD 198

[Close]

BLAST of CSPI04G04310 vs. TrEMBL
Match: A0A061G047_THECC (Mediator of RNA polymerase II transcription subunit 31 isoform 2 OS=Theobroma cacao GN=TCM_014808 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 3.3e-79
Identity = 154/204 (75.49%), Postives = 170/204 (83.33%), Query Frame = 1


Query: 9   EEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKY 68
           +  +++PSSP  VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDEAFIGYLKY
Sbjct: 8   DNASNSPSSPKTVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKY 67

Query: 69  LQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 128
           LQYWQRPEYIKFIMYPHCL+FLELLQN++FRN MAHP NKELAHRQQF+FWKNYRNNRLK
Sbjct: 68  LQYWQRPEYIKFIMYPHCLYFLELLQNASFRNGMAHPVNKELAHRQQFFFWKNYRNNRLK 127

Query: 129 HILPRPLPEPAALP---PPVTAPPQAA---VPAPTSAPNMAASPAAPALSPMQYGIPPGP 188
            ILP+P PEP A P   PP   PPQAA   VPA T A   A+   + ALSPM YG+PPG 
Sbjct: 128 FILPKPPPEPVAAPAPLPPTAVPPQAAMPPVPATTIAMTSASPAPSSALSPMPYGLPPGS 187

Query: 189 GLPKNDMKGAGIDRRKRKHERSMT 207
            L KNDM+ +GIDRRKRK+ERS+T
Sbjct: 188 VLAKNDMRNSGIDRRKRKYERSLT 211

[Close]

BLAST of CSPI04G04310 vs. TrEMBL
Match: A0A0J8CG17_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g104190 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 5.6e-79
Identity = 154/202 (76.24%), Postives = 171/202 (84.65%), Query Frame = 1


Query: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MAS++   ++ +++PS   NVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY +DE
Sbjct: 1   MASSNDA-DDTSNSPSLTQNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFDDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120
           AFIGYLKYLQYWQ+PEYIKFIMYPHCLFFLELLQN+NFRNAMAHPG+KELAHRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQQPEYIKFIMYPHCLFFLELLQNANFRNAMAHPGSKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEP   PP  TA P    P P + P  A S ++PALSPMQY IPP
Sbjct: 121 NYRNNRLKHILPRPLPEPDPAPPASTAAPP---PLPPAIP--ATSASSPALSPMQYAIPP 180

Query: 181 GPGLPKNDMKGAGIDRRKRKHE 203
           G G+ KNDM+ +G DRRKRK E
Sbjct: 181 GSGVAKNDMRNSGTDRRKRKKE 196

[Close]

BLAST of CSPI04G04310 vs. TrEMBL
Match: A0A0D2N5R9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G052400 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.3e-78
Identity = 152/202 (75.25%), Postives = 168/202 (83.17%), Query Frame = 1


Query: 9   EEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKY 68
           +  +D PSSP NVYKDPDDGRQRFLLELEF+QCLANPTYIHYLAQNRY EDEAFIGYLKY
Sbjct: 8   DNASDTPSSPKNVYKDPDDGRQRFLLELEFLQCLANPTYIHYLAQNRYFEDEAFIGYLKY 67

Query: 69  LQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 128
           LQYWQRPEYIKFIMYPHCL+FLELLQN+NFRNAMAHP NKE+AHRQQF+FWKNYRNNRLK
Sbjct: 68  LQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPANKEVAHRQQFFFWKNYRNNRLK 127

Query: 129 HILPRPLPEPAALP---PPVTAPPQAAVPAPTSAPNMAASPAAPAL--SPMQYGIPPGPG 188
            ILP+P PE    P   PP +APPQ ++PA   A  M  +P APA   SPM YG+P G  
Sbjct: 128 FILPKPPPEEVPTPAPLPPASAPPQQSLPASNIA--MTTAPPAPASTHSPMPYGLPSGSA 187

Query: 189 LPKNDMKGAGIDRRKRKHERSM 206
           L KNDM+ +GIDRRKRKHERS+
Sbjct: 188 LAKNDMRNSGIDRRKRKHERSL 207

[Close]

BLAST of CSPI04G04310 vs. TAIR10
Match: AT5G19910.2 (AT5G19910.2 SOH1 family protein)

HSP 1 Score: 257.7 bits (657), Expect = 6.2e-69
Identity = 146/233 (62.66%), Postives = 158/233 (67.81%), Query Frame = 1


Query: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MAS   + ++ ++ PS P N YKDPD GRQRFLLELEF+QCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNK------------ 120
           AFIGYLKYLQYWQRPEYIKFIMYPHCL+FLELLQN NFR AMAHP NK            
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKWFKMLGYWCFWN 120

Query: 121 ------------------ELAHRQQFYFWKNYRNNRLKHILPRPLPEPAALPPPVTAPPQ 180
                             ELAHRQQFY+WKNYRNNRLKHILPRPLPEP    PPV AP  
Sbjct: 121 VFWGLVEFRVSLVQNLAYELAHRQQFYYWKNYRNNRLKHILPRPLPEPVPPQPPV-APST 180

Query: 181 AAVPAPTSAPNMAASPAAPALSPMQYGIPPGPGLPKND---MKGAGIDRRKRK 201
           +  PAP+     A +  +PALSPMQY       L KND   M   GIDRRKRK
Sbjct: 181 SLPPAPS-----ATAALSPALSPMQY----NNMLSKNDTRNMGATGIDRRKRK 223

[Close]

BLAST of CSPI04G04310 vs. NCBI nr
Match: gi|449458311|ref|XP_004146891.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis sativus])

HSP 1 Score: 417.9 bits (1073), Expect = 1.0e-113
Identity = 205/206 (99.51%), Postives = 206/206 (100.00%), Query Frame = 1


Query: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSP+QYGIPP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPIQYGIPP 180

Query: 181 GPGLPKNDMKGAGIDRRKRKHERSMT 207
           GPGLPKNDMKGAGIDRRKRKHERSMT
Sbjct: 181 GPGLPKNDMKGAGIDRRKRKHERSMT 206

[Close]

BLAST of CSPI04G04310 vs. NCBI nr
Match: gi|659107763|ref|XP_008453845.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis melo])

HSP 1 Score: 401.4 bits (1030), Expect = 9.8e-109
Identity = 197/206 (95.63%), Postives = 199/206 (96.60%), Query Frame = 1


Query: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60
           MASNHGL EE ADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE
Sbjct: 1   MASNHGLNEEAADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEPAALPPPV+APPQA VPAPT AP +AASPA  ALSPMQYGIPP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVSAPPQAPVPAPTPAPTVAASPATAALSPMQYGIPP 180

Query: 181 GPGLPKNDMKGAGIDRRKRKHERSMT 207
           GPGLPKNDMKGAGIDRRKRKHERSMT
Sbjct: 181 GPGLPKNDMKGAGIDRRKRKHERSMT 206

[Close]

BLAST of CSPI04G04310 vs. NCBI nr
Match: gi|567889026|ref|XP_006437035.1| (hypothetical protein CICLE_v10032791mg [Citrus clementina])

HSP 1 Score: 310.8 bits (795), Expect = 1.7e-81
Identity = 156/194 (80.41%), Postives = 166/194 (85.57%), Query Frame = 1


Query: 9   EEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKY 68
           EE +D PSSP  VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDEAFIGYLKY
Sbjct: 8   EEASDAPSSPKKVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKY 67

Query: 69  LQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 128
           LQYWQRPEYIKFIMYPHCL+FLELLQN+NFRNAMAHP NKELAHRQQF+FWKNYRNNRLK
Sbjct: 68  LQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPANKELAHRQQFFFWKNYRNNRLK 127

Query: 129 HILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPPGPGLPKND 188
           HILPRPLPEP+  PPP  APP    PAP     + A+P  PALSPMQYGIPPG  L KND
Sbjct: 128 HILPRPLPEPSEAPPPAAAPP--LPPAPPVLTPVTAAP-GPALSPMQYGIPPGSALMKND 187

Query: 189 MKGAGIDRRKRKHE 203
           M+ + IDRRKRK +
Sbjct: 188 MRSSSIDRRKRKKD 198

[Close]

BLAST of CSPI04G04310 vs. NCBI nr
Match: gi|720044213|ref|XP_010269818.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 31 isoform X1 [Nelumbo nucifera])

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-81
Identity = 157/207 (75.85%), Postives = 169/207 (81.64%), Query Frame = 1


Query: 6   GLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGY 65
           G + ++   PS P N+YKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDEAFIGY
Sbjct: 4   GKESDDVQTPSMPKNIYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGY 63

Query: 66  LKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNN 125
           LKYLQYWQRPEY+KFIMYPHCLFFLELLQNSNFRNAMAHPG+KE+AHRQQF+FWKNYRNN
Sbjct: 64  LKYLQYWQRPEYVKFIMYPHCLFFLELLQNSNFRNAMAHPGSKEIAHRQQFFFWKNYRNN 123

Query: 126 RLKHILPRPLPEPAALPP---------PVTAPPQAAVPAPTSAPNMA-ASPAAPALSPMQ 185
           RLKHILPRPLPEP A PP         P T  P AA PAP   P  A A+P A ALSPM 
Sbjct: 124 RLKHILPRPLPEPVAAPPAPIPPPAPLPATNVPVAASPAPVLVPVPAPAAPPASALSPMP 183

Query: 186 YGIPPGPGLPKNDMKGAGIDRRKRKHE 203
           YG+PPGP L KND + +GIDRRKRK E
Sbjct: 184 YGLPPGPTLSKNDPRNSGIDRRKRKKE 210

[Close]

BLAST of CSPI04G04310 vs. NCBI nr
Match: gi|802770058|ref|XP_012090529.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Jatropha curcas])

HSP 1 Score: 308.5 bits (789), Expect = 8.7e-81
Identity = 154/197 (78.17%), Postives = 168/197 (85.28%), Query Frame = 1


Query: 9   EEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDEAFIGYLKY 68
           ++  DNPSSP N+YKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDEAFIGYLKY
Sbjct: 8   DDTLDNPSSPKNIYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKY 67

Query: 69  LQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWKNYRNNRLK 128
           LQYWQRPEY+KFIMYPHCL+FLELLQN+NFRNAMAHPGNKELAHRQQF+FWKNYRNNRLK
Sbjct: 68  LQYWQRPEYLKFIMYPHCLYFLELLQNANFRNAMAHPGNKELAHRQQFFFWKNYRNNRLK 127

Query: 129 HILPRPLPEPAALPP---PVTAPPQAAVPAPTSAPNMAASPAAPALSPMQYGIPPGPGLP 188
           HILPRPLPEPA  PP   P+  P Q   P P +   + A+PA+ ALSPM YG+ PG  L 
Sbjct: 128 HILPRPLPEPAPAPPASAPLPPPVQPVPPMPATTIPVPAAPAS-ALSPMPYGMAPGSALA 187

Query: 189 KNDMKGAGIDRRKRKHE 203
           KNDM+ AGIDRRKRK E
Sbjct: 188 KNDMRNAGIDRRKRKKE 203

The following BLAST results are available for this feature:

BLAST of CSPI04G04310 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MED31_ARATH	1.9e-72	71.92	Mediator of RNA polymerase II transcription subunit 31 OS=Arabidopsis thaliana G...	[more]
MED31_DICDI	1.3e-31	58.76	Putative mediator of RNA polymerase II transcription subunit 31 OS=Dictyostelium...	[more]
MED31_DANRE	1.4e-25	49.07	Mediator of RNA polymerase II transcription subunit 31 OS=Danio rerio GN=med31 P...	[more]
MED31_BOVIN	3.3e-24	48.57	Mediator of RNA polymerase II transcription subunit 31 OS=Bos taurus GN=MED31 PE...	[more]
MED31_MOUSE	4.4e-24	48.57	Mediator of RNA polymerase II transcription subunit 31 OS=Mus musculus GN=Med31 ...	[more]

BLAST of CSPI04G04310 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ88_CUCSA	7.1e-114	99.51	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025160 PE=4 SV=1	[more]
V4TKG6_9ROSI	1.2e-81	80.41	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10032791mg PE=4 SV=1	[more]
A0A061G047_THECC	3.3e-79	75.49	Mediator of RNA polymerase II transcription subunit 31 isoform 2 OS=Theobroma ca...	[more]
A0A0J8CG17_BETVU	5.6e-79	76.24	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_5g104190 PE=4 S...	[more]
A0A0D2N5R9_GOSRA	1.3e-78	75.25	Uncharacterized protein OS=Gossypium raimondii GN=B456_001G052400 PE=4 SV=1	[more]

BLAST of CSPI04G04310 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19910.2	6.2e-69	62.66	SOH1 family protein	[more]

BLAST of CSPI04G04310 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449458311\|ref\|XP_004146891.1\|	1.0e-113	99.51	PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis sativ...	[more]
gi\|659107763\|ref\|XP_008453845.1\|	9.8e-109	95.63	PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis melo]	[more]
gi\|567889026\|ref\|XP_006437035.1\|	1.7e-81	80.41	hypothetical protein CICLE_v10032791mg [Citrus clementina]	[more]
gi\|720044213\|ref\|XP_010269818.1\|	2.3e-81	75.85	PREDICTED: mediator of RNA polymerase II transcription subunit 31 isoform X1 [Ne...	[more]
gi\|802770058\|ref\|XP_012090529.1\|	8.7e-81	78.17	PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Jatropha curc...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008831	Mediator_Med31

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **Molecular Function**
Term	Definition
GO:0001104	RNA polymerase II transcription cofactor activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016592	mediator complex

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription from RNA polymerase II promoter
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0001104	RNA polymerase II transcription cofactor activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0003712	transcription cofactor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G04310.1	CSPI04G04310.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008831	Mediator complex, subunit Med31	PANTHER	PTHR13186	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT SOH1	coord: 1..175 score: 2.1
IPR008831	Mediator complex, subunit Med31	PFAM	PF05669	Med31	coord: 30..122 score: 6.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G04310	Cla010693	Watermelon (97103) v1	cpiwmB315
CSPI04G04310	Csa4G025160	Cucumber (Chinese Long) v2	cpicuB182
CSPI04G04310	MELO3C017626	Melon (DHL92) v3.5.1	cpimeB310
CSPI04G04310	ClCG07G017200	Watermelon (Charleston Gray)	cpiwcgB336
CSPI04G04310	Lsi07G001380	Bottle gourd (USVL1VR-Ls)	cpilsiB303
CSPI04G04310	MELO3C017626.2	Melon (DHL92) v3.6.1	cpimedB301
CSPI04G04310	CsaV3_4G004400	Cucumber (Chinese Long) v3	cpicucB213
CSPI04G04310	Cla97C07G144220	Watermelon (97103) v2	cpiwmbB326
CSPI04G04310	Bhi01G001986	Wax gourd	cpiwgoB378
CSPI04G04310	Cucsa.093930	Cucumber (Gy14) v1	cgycpiB103
CSPI04G04310	CmaCh04G003740	Cucurbita maxima (Rimu)	cmacpiB716
CSPI04G04310	CmaCh16G002300	Cucurbita maxima (Rimu)	cmacpiB337
CSPI04G04310	CmoCh04G003950	Cucurbita moschata (Rifu)	cmocpiB708
CSPI04G04310	Cp4.1LG01g03360	Cucurbita pepo (Zucchini)	cpecpiB398
CSPI04G04310	CsGy4G004220	Cucumber (Gy14) v2	cgybcpiB168
CSPI04G04310	Carg16772	Silver-seed gourd	carcpiB0906
CSPI04G04310	Carg08048	Silver-seed gourd	carcpiB0408

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G04310	Cucurbita pepo (Zucchini)	cpecpiB220
CSPI04G04310	Cucurbita pepo (Zucchini)	cpecpiB731
CSPI04G04310	Bottle gourd (USVL1VR-Ls)	cpilsiB243
CSPI04G04310	Cucumber (Gy14) v2	cgybcpiB222
CSPI04G04310	Silver-seed gourd	carcpiB0746
CSPI04G04310	Cucumber (Chinese Long) v3	cpicucB234
CSPI04G04310	Watermelon (97103) v2	cpiwmbB274
CSPI04G04310	Wild cucumber (PI 183967)	cpicpiB156
CSPI04G04310	Cucurbita moschata (Rifu)	cmocpiB328
CSPI04G04310	Cucurbita moschata (Rifu)	cmocpiB604
CSPI04G04310	Cucumber (Chinese Long) v2	cpicuB194
CSPI04G04310	Watermelon (Charleston Gray)	cpiwcgB298