Carg16467 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16467
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	ralf-like 11
Location	Cucurbita_argyrosperma_scaffold_130 : 763104 .. 763386 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCGTTACCGATATGTTTAGCTTTAATTACGATGCTGATGACAAGCCAACAAGTGATGACGAACCAAGAAGCAACATCACAAATAAAATATCTTAAACATTTAGAATGTCATCATCCTAACCCACCTGCACATTGTTCTTTATCTGGAGTTAAGGGCTATCAACATGTTCCTGCTAATCCCTATGATCGAGGATGCTCTGCTATCCATCGATGTCGAGGAGGAAGTTAATCAATATTTGAAATTATTGTTTTCTTCTATAGGAAGGAAAAATGAGTAA

mRNA sequence

ATGAAGTCGTTACCGATATGTTTAGCTTTAATTACGATGCTGATGACAAGCCAACAAGTGATGACGAACCAAGAAGCAACATCACAAATAAAATATCTTAAACATTTAGAATGTCATCATCCTAACCCACCTGCACATTGTTCTTTATCTGGAGTTAAGGGCTATCAACATGTTCCTGCTAATCCCTATGATCGAGGATGCTCTGCTATCCATCGATGTCGAGGAGGAAGAAGGAAAAATGAGTAA

Coding sequence (CDS)

ATGAAGTCGTTACCGATATGTTTAGCTTTAATTACGATGCTGATGACAAGCCAACAAGTGATGACGAACCAAGAAGCAACATCACAAATAAAATATCTTAAACATTTAGAATGTCATCATCCTAACCCACCTGCACATTGTTCTTTATCTGGAGTTAAGGGCTATCAACATGTTCCTGCTAATCCCTATGATCGAGGATGCTCTGCTATCCATCGATGTCGAGGAGGAAGAAGGAAAAATGAGTAA

Protein sequence

MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPANPYDRGCSAIHRCRGGRRKNE

Homology

[Close]

BLAST of Carg16467 vs. NCBI nr
Match: XP_023533089.1 (protein RALF-like 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-07
Identity = 38/77 (49.35%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLE-CHHPNPPAHCSLSGVKGYQHVP 60
          M+S  IC+ LI+ML+ SQ+V       S+ K+LK  + C  PNPP  CS+        VP
Sbjct: 1  MRSWLICIVLISMLVVSQEV------ASETKFLKFPDPCQQPNPPLGCSIGNA-----VP 60

Query: 61 ANPYDRGCSAIHRCRGG 77
          ANPY RGCS I RCRGG
Sbjct: 61 ANPYRRGCSKITRCRGG 66

[Close]

BLAST of Carg16467 vs. NCBI nr
Match: XP_022947821.1 (protein RALF-like 10 [Cucurbita moschata])

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-07
Identity = 39/77 (50.65%), Postives = 45/77 (58.44%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLE-CHHPNPPAHCSLSGVKGYQHVP 60
          M+S  ICL LI ML+ SQ V       S+ K+LK  + C  PNPP  CS+        VP
Sbjct: 1  MRSWLICLVLIGMLVVSQDV------ASETKFLKFPDPCQQPNPPPGCSIGNA-----VP 60

Query: 61 ANPYDRGCSAIHRCRGG 77
          ANPY RGCS I RCRGG
Sbjct: 61 ANPYRRGCSKITRCRGG 66

[Close]

BLAST of Carg16467 vs. NCBI nr
Match: KGN66777.1 (hypothetical protein Csa_1G690160 [Cucumis sativus])

HSP 1 Score: 63.2 bits (152), Expect = 4.8e-07
Identity = 39/75 (52.00%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MKS  IC+  I+ML+ SQ+V      T+ +K+     C  P+PP  CS S VK   H P 
Sbjct: 1  MKSWLICVVFISMLVMSQEVGAQ---TNHLKFPN--PCDLPSPPPSCS-SKVK--DHAPT 60

Query: 61 NPYDRGCSAIHRCRG 76
          NPYDRGCSAIHRCRG
Sbjct: 61 NPYDRGCSAIHRCRG 67

[Close]

BLAST of Carg16467 vs. NCBI nr
Match: KGN61625.1 (hypothetical protein Csa_2G191310 [Cucumis sativus])

HSP 1 Score: 54.7 bits (130), Expect = 1.7e-04
Identity = 35/77 (45.45%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLE-CHHPNPPAHCSLSGVKGYQHVP 60
          MKS  ICLALI ML+  Q+V       ++ K+LK ++ C+ P+PP  C        +   
Sbjct: 1  MKSWLICLALIGMLVVGQEV------AAETKHLKFIDPCNQPSPPPTCP----NRDKVEE 60

Query: 61 ANPYDRGCSAIHRCRGG 77
          ANPY+R CSAI+RCRGG
Sbjct: 61 ANPYNRDCSAINRCRGG 67

[Close]

BLAST of Carg16467 vs. NCBI nr
Match: OAP10003.1 (RALFL12 [Arabidopsis thaliana])

HSP 1 Score: 53.5 bits (127), Expect = 3.8e-04
Identity = 30/74 (40.54%), Postives = 41/74 (55.41%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  ICL LI  ++ ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWVICLLLICAVVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. TAIR10
Match: AT2G19030.1 (ralf-like 11)

HSP 1 Score: 51.2 bits (121), Expect = 3.4e-07
Identity = 29/74 (39.19%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  ICL +I   + ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWLICLLVICAAVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. TAIR10
Match: AT2G19045.1 (RALF-like 13)

HSP 1 Score: 50.4 bits (119), Expect = 5.9e-07
Identity = 29/74 (39.19%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  ICL +I   + ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWVICLLVICAAVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPNPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. TAIR10
Match: AT2G19040.1 (RALF-like 12)

HSP 1 Score: 47.8 bits (112), Expect = 3.8e-06
Identity = 28/74 (37.84%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  I L +I  ++ ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWVIGLLVICAVVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. TAIR10
Match: AT2G19020.1 (ralf-like 10)

HSP 1 Score: 43.9 bits (102), Expect = 5.5e-05
Identity = 27/74 (36.49%), Postives = 38/74 (51.35%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+L ICL +I   + +  V + ++    + Y    +C  PNPP  C   G +     PA
Sbjct: 1  MKALVICLLVIFAAVIAVPVESRRK---HLDYGVITKCAGPNPPPGCYPPGAQQKNPTPA 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS I RC+
Sbjct: 61 NEYRRGCSKITRCK 71

[Close]

BLAST of Carg16467 vs. Swiss-Prot
Match: sp|O64466|RLF11_ARATH (Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.2e-06
Identity = 29/74 (39.19%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  ICL +I   + ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWLICLLVICAAVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. Swiss-Prot
Match: sp|F4ISE2|RLF13_ARATH (Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.1e-05
Identity = 29/74 (39.19%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  ICL +I   + ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWVICLLVICAAVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGAEQKNPNPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. Swiss-Prot
Match: sp|F4ISE1|RLF12_ARATH (Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 6.9e-05
Identity = 28/74 (37.84%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  I L +I  ++ ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWVIGLLVICAVVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. Swiss-Prot
Match: sp|O65919|RLF10_ARATH (Protein RALF-like 10 OS=Arabidopsis thaliana OX=3702 GN=RALFL10 PE=2 SV=1)

HSP 1 Score: 43.9 bits (102), Expect = 9.9e-04
Identity = 27/74 (36.49%), Postives = 38/74 (51.35%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+L ICL +I   + +  V + ++    + Y    +C  PNPP  C   G +     PA
Sbjct: 1  MKALVICLLVIFAAVIAVPVESRRK---HLDYGVITKCAGPNPPPGCYPPGAQQKNPTPA 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS I RC+
Sbjct: 61 NEYRRGCSKITRCK 71

[Close]

BLAST of Carg16467 vs. TrEMBL
Match: tr|A0A0A0M0X3|A0A0A0M0X3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-07
Identity = 39/75 (52.00%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MKS  IC+  I+ML+ SQ+V      T+ +K+     C  P+PP  CS S VK   H P 
Sbjct: 1  MKSWLICVVFISMLVMSQEVGAQ---TNHLKFPN--PCDLPSPPPSCS-SKVK--DHAPT 60

Query: 61 NPYDRGCSAIHRCRG 76
          NPYDRGCSAIHRCRG
Sbjct: 61 NPYDRGCSAIHRCRG 67

[Close]

BLAST of Carg16467 vs. TrEMBL
Match: tr|A0A0A0LI94|A0A0A0LI94_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=4 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.1e-04
Identity = 35/77 (45.45%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLE-CHHPNPPAHCSLSGVKGYQHVP 60
          MKS  ICLALI ML+  Q+V       ++ K+LK ++ C+ P+PP  C        +   
Sbjct: 1  MKSWLICLALIGMLVVGQEV------AAETKHLKFIDPCNQPSPPPTCP----NRDKVEE 60

Query: 61 ANPYDRGCSAIHRCRGG 77
          ANPY+R CSAI+RCRGG
Sbjct: 61 ANPYNRDCSAINRCRGG 67

[Close]

BLAST of Carg16467 vs. TrEMBL
Match: tr|A0A178VY78|A0A178VY78_ARATH (RALFL12 OS=Arabidopsis thaliana OX=3702 GN=AXX17_At2g14390 PE=4 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 2.5e-04
Identity = 30/74 (40.54%), Postives = 41/74 (55.41%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  ICL LI  ++ ++ V    E+ + I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWVICLLLICAVVIAEPV----ESRNYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

[Close]

BLAST of Carg16467 vs. TrEMBL
Match: tr|M4DF40|M4DF40_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=4 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 7.3e-04
Identity = 35/71 (49.30%), Postives = 39/71 (54.93%), Query Frame = 0


Query: 10 LITMLMTSQQVMTNQ-EATSQIKYLK-HL--ECHHPNPPAHCSLSGVKGYQHVPANPYDR 69
          +I ML+    V+  Q EA    KYL  H+   CH PNPPA C          VPAN Y R
Sbjct: 5  VILMLVICAAVIVEQSEAKKDRKYLSPHIFDPCHGPNPPAGC--HPPHDGPPVPANKYTR 64

Query: 70 GCSAIHRCRGG 77
          GCS IHRCRGG
Sbjct: 65 GCSKIHRCRGG 73

[Close]

BLAST of Carg16467 vs. TrEMBL
Match: tr|B2Y4P5|B2Y4P5_ARAHH (Putative rapid alkalinization factor (RALF) family protein OS=Arabidopsis halleri subsp. halleri OX=81971 GN=7C17.2 PE=4 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 7.3e-04
Identity = 29/74 (39.19%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 1  MKSLPICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPA 60
          MK+  ICL +I  ++ ++ V    E+   I+Y    +C  PNPP  C+  G +     P 
Sbjct: 1  MKAWVICLLVICAVVIAEPV----ESREYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPV 60

Query: 61 NPYDRGCSAIHRCR 75
          N Y RGCS IHRCR
Sbjct: 61 NEYSRGCSKIHRCR 70

The following BLAST results are available for this feature:

BLAST of Carg16467 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023533089.1	1.7e-07	49.35	protein RALF-like 10 [Cucurbita pepo subsp. pepo]	[more]
XP_022947821.1	3.7e-07	50.65	protein RALF-like 10 [Cucurbita moschata]	[more]
KGN66777.1	4.8e-07	52.00	hypothetical protein Csa_1G690160 [Cucumis sativus]	[more]
KGN61625.1	1.7e-04	45.45	hypothetical protein Csa_2G191310 [Cucumis sativus]	[more]
OAP10003.1	3.8e-04	40.54	RALFL12 [Arabidopsis thaliana]	[more]

BLAST of Carg16467 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G19030.1	3.4e-07	39.19	ralf-like 11	[more]
AT2G19045.1	5.9e-07	39.19	RALF-like 13	[more]
AT2G19040.1	3.8e-06	37.84	RALF-like 12	[more]
AT2G19020.1	5.5e-05	36.49	ralf-like 10	[more]

BLAST of Carg16467 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O64466\|RLF11_ARATH	6.2e-06	39.19	Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1	[more]
sp\|F4ISE2\|RLF13_ARATH	1.1e-05	39.19	Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1	[more]
sp\|F4ISE1\|RLF12_ARATH	6.9e-05	37.84	Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1	[more]
sp\|O65919\|RLF10_ARATH	9.9e-04	36.49	Protein RALF-like 10 OS=Arabidopsis thaliana OX=3702 GN=RALFL10 PE=2 SV=1	[more]

BLAST of Carg16467 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0M0X3\|A0A0A0M0X3_CUCSA	3.2e-07	52.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=4 SV=1	[more]
tr\|A0A0A0LI94\|A0A0A0LI94_CUCSA	1.1e-04	45.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=4 SV=1	[more]
tr\|A0A178VY78\|A0A178VY78_ARATH	2.5e-04	40.54	RALFL12 OS=Arabidopsis thaliana OX=3702 GN=AXX17_At2g14390 PE=4 SV=1	[more]
tr\|M4DF40\|M4DF40_BRARP	7.3e-04	49.30	Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=4 SV=1	[more]
tr\|B2Y4P5\|B2Y4P5_ARAHH	7.3e-04	39.19	Putative rapid alkalinization factor (RALF) family protein OS=Arabidopsis haller...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16467-RA	Carg16467-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 36..75 e-value: 3.5E-7 score: 30.9
None	No IPR available	PANTHER	PTHR34270	FAMILY NOT NAMED	coord: 6..78

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg16467	CmaCh11G009620	Cucurbita maxima (Rimu)	carcmaB1252
Carg16467	CmoCh11G009850	Cucurbita moschata (Rifu)	carcmoB1222
Carg16467	Cp4.1LG04g02100	Cucurbita pepo (Zucchini)	carcpeB1261

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg16467	Cucumber (Gy14) v2	carcgybB0899
Carg16467	Cucumber (Gy14) v1	carcgyB1032
Carg16467	Cucurbita maxima (Rimu)	carcmaB1251
Carg16467	Cucurbita moschata (Rifu)	carcmoB1221
Carg16467	Wild cucumber (PI 183967)	carcpiB0949
Carg16467	Cucumber (Chinese Long) v2	carcuB0927
Carg16467	Cucumber (Chinese Long) v3	carcucB0949
Carg16467	Bottle gourd (USVL1VR-Ls)	carlsiB830
Carg16467	Melon (DHL92) v3.6.1	carmedB0904
Carg16467	Melon (DHL92) v3.6.1	carmedB0906
Carg16467	Wax gourd	carwgoB1057