Carg16279 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16279
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	Cucurbita_argyrosperma_scaffold_077 : 490981 .. 491948 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGATGGTTCTCGATCTAAGTTCTATGAATTTCGTCACGAACTTCGTCTCCAATTTCGAGTTCTTACATTTGCCTCTTAATCTCCTGATGTAATTCGTCCAGAAACATCGCTAATTCACTCCGTTGAATCGAAGGAACAAACTACAATGATTTATTAAAGAAACTAAAAGACGGATTCGTTCTGATTTTTCAGTAACAGTGCCGGCAGATTCTGTTACGAACGTGCAATTTCTGAAGACGGAATGGAGATGACGTTCGCTACGAATTTATCTAGGTAAGTACGCAGAACAAAATTTTTCATGTCTAATCATGAGAACTTCTGCCATTATTAATTTAATCGATCATGTAGGTCATTTTCTTTTCACGAAACTTTTGTTGAATAAGATGATCGAAACAGCCAAATCAACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAATATCCACAGCCGGTTTTCCGGCGACGTATTCGAATGTCTCGGTCAGATAAGCCAGAGCAACAGGTAAGATTAATTTTCCTTTGCTTGCGTATCAATGGCGGTCTGATAGCGATACTGATATTAACTCGCAGCAGAGAGTACGACGCGACACGTGCTTACACCGTCCAACTCGCTCACAGACTTCAGGTTCTTCCTCAATATCAATTGGTATGTTCATAGAACGATTTCACGCTCGACTTTGTTTTTGACGAAATCGGAACAGGAAATGGAGGCAAATGTTACAGTAAACTGTGTTCATCCGGGCGTAGTGAAAACCAAATTAAACAGAGATCGTGAAGGCTTCGTCATAGGTATCCAAACAATCGAAACATTTCTATATAACTAATTAATGAATGAGAAATGCATATTTGATTCTCTACATGGCGTCAAATTCCTCAAGGAGCAGCAACAAGTTGCAAAGTAGCCGCGAATCGGAGCGTGGATAATGTGAATGGCAAATACTTTGCGGATTGCAATGATTAA

mRNA sequence

ATGGTGATGGTTCTCGATCTAAGTTCTATGAATTTCGTCACGAACTTCGTCTCCAATTTCGAGTTCTTACATTTGCCTCTTAATCTCCTGATTAACAGTGCCGGCAGATTCTGTTACGAACGTGCAATTTCTGAAGACGGAATGGAGATGACGTTCGCTACGAATTTATCTAGCCAAATCAACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAATATCCACAGCCGGTTTTCCGGCGACGTATTCGAATGTCTCGGTCAGATAAGCCAGAGCAACAGGTAAGATTAATTTTCCTTTGCTTGCGTATCAATGGCGGTCTGATAGCGATACTGATATTAACTCGCAGCAGAGAGTACGACGCGACACGTGCTTACACCGTCCAACTCGCTCACAGACTTCAGGAAATGGAGGCAAATGTTACAGTAAACTGTGTTCATCCGGGCGTAGTGAAAACCAAATTAAACAGAGATCGTGAAGGCTTCGTCATAGGAGCAGCAACAAGTTGCAAAGTAGCCGCGAATCGGAGCGTGGATAATGTGAATGGCAAATACTTTGCGGATTGCAATGATTAA

Coding sequence (CDS)

ATGGTGATGGTTCTCGATCTAAGTTCTATGAATTTCGTCACGAACTTCGTCTCCAATTTCGAGTTCTTACATTTGCCTCTTAATCTCCTGATTAACAGTGCCGGCAGATTCTGTTACGAACGTGCAATTTCTGAAGACGGAATGGAGATGACGTTCGCTACGAATTTATCTAGCCAAATCAACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAATATCCACAGCCGGTTTTCCGGCGACGTATTCGAATGTCTCGGTCAGATAAGCCAGAGCAACAGGTAAGATTAATTTTCCTTTGCTTGCGTATCAATGGCGGTCTGATAGCGATACTGATATTAACTCGCAGCAGAGAGTACGACGCGACACGTGCTTACACCGTCCAACTCGCTCACAGACTTCAGGAAATGGAGGCAAATGTTACAGTAAACTGTGTTCATCCGGGCGTAGTGAAAACCAAATTAAACAGAGATCGTGAAGGCTTCGTCATAGGAGCAGCAACAAGTTGCAAAGTAGCCGCGAATCGGAGCGTGGATAATGTGAATGGCAAATACTTTGCGGATTGCAATGATTAA

Protein sequence

MVMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQINGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILILTRSREYDATRAYTVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVIGAATSCKVAANRSVDNVNGKYFADCND

Homology

[Close]

BLAST of Carg16279 vs. NCBI nr
Match: XP_022952610.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 239.6 bits (610), Expect = 8.9e-60
Identity = 132/193 (68.39%), Postives = 147/193 (76.17%), Query Frame = 0


Query: 3   MVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQING 62
           MVLDLSSMNFV NFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN       
Sbjct: 1   MVLDLSSMNFVPNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNYRGHF-- 60

Query: 63  NPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIF--LCLRINGGLIAIL--ILTRSR 122
                      + + +  + I  ++S   + ++  +   +  R++G +I  L  I   +R
Sbjct: 61  ----------LFTKLLLNKMIETAKSTGIQGRIVNVTSNIHSRLSGDVIECLGQISQSNR 120

Query: 123 EYDATRAYTVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVIGAATSCKVAANRSV 182
           EYDATRAYTVQLAHRLQEMEANVTVNCVHP VVKTKLNRDREGFVIGAATSCKVAANRSV
Sbjct: 121 EYDATRAYTVQLAHRLQEMEANVTVNCVHPRVVKTKLNRDREGFVIGAATSCKVAANRSV 180

Query: 183 DNVNGKYFADCND 192
           DNVNGKYFADCND
Sbjct: 181 DNVNGKYFADCND 181

[Close]

BLAST of Carg16279 vs. NCBI nr
Match: KGN55679.1 (hypothetical protein Csa_3G003470 [Cucumis sativus])

HSP 1 Score: 218.8 bits (556), Expect = 1.6e-53
Identity = 130/222 (58.56%), Postives = 154/222 (69.37%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQIN 61
           VMVLDLSS+N V NFVS FE L+LPLNLL++ +     E ++ +D        + + +I+
Sbjct: 93  VMVLDLSSLNSVMNFVSEFESLNLPLNLLMSFS---LDETSVEQD--------DRNGRID 152

Query: 62  GNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQ---QVRLIFLCLRINGG-LIAILILTRS 121
           GNPRPNSECDVQ+PQ VFRRRIR+S+ DKPEQ   QVRLI L L ING  +I ILI+ +S
Sbjct: 153 GNPRPNSECDVQHPQLVFRRRIRISQPDKPEQQQVQVRLILLSLCINGAPIIVILIIIQS 212

Query: 122 REYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFV---- 181
           REYDATRAY          TVQLAHRLQEM+ANVTVNCVHPGVV+T LNRDREGF+    
Sbjct: 213 REYDATRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGVVRTNLNRDREGFIKDLI 272

Query: 182 --------------IGAATSCKVAANRSVDNVNGKYFADCND 192
                           AATSC VA +R V+NVNGKYFADCN+
Sbjct: 273 FFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKYFADCNE 303

[Close]

BLAST of Carg16279 vs. NCBI nr
Match: XP_022145404.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X4 [Momordica charantia])

HSP 1 Score: 189.1 bits (479), Expect = 1.4e-44
Identity = 116/218 (53.21%), Postives = 128/218 (58.72%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQIN 61
           VM LDLSS+N V NFVS FE L+LPLNLLIN+AGRFCYE AISEDG+EMTF TN      
Sbjct: 95  VMELDLSSLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLGIQG 154

Query: 62  GNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILILTRSREYD 121
                 S     +   +F    R+SRS+                           SREYD
Sbjct: 155 RIVNLTSNIHSWFSGDIFEYLGRISRSN---------------------------SREYD 214

Query: 122 ATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVI------- 181
           ATRAY          TVQLAHRLQE EANVTVNCVHPGVV+TKLNRDREGFV        
Sbjct: 215 ATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMA 274

Query: 182 -----------GAATSCKVAANRSVDNVNGKYFADCND 192
                      GAATSC VA NR+V+NVNGKYFADCN+
Sbjct: 275 SKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCNE 285

[Close]

BLAST of Carg16279 vs. NCBI nr
Match: XP_022145401.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 188.3 bits (477), Expect = 2.3e-44
Identity = 118/228 (51.75%), Postives = 140/228 (61.40%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQIN 61
           VM LDLSS+N V NFVS FE L+LPLNLLIN+AGRFCYE AISEDG+EMTF TN   Q  
Sbjct: 95  VMELDLSSLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYI 154

Query: 62  GNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQ----QVRLIFLCLRI----NGGLIAIL- 121
           G         + +       ++ +++  +  +    Q R++ L   I    +G +   L 
Sbjct: 155 GE-------QILFSGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLG 214

Query: 122 -ILTRSREYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREG 181
            I   +REYDATRAY          TVQLAHRLQE EANVTVNCVHPGVV+TKLNRDREG
Sbjct: 215 RISRSNREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDREG 274

Query: 182 FVI------------------GAATSCKVAANRSVDNVNGKYFADCND 192
           FV                   GAATSC VA NR+V+NVNGKYFADCN+
Sbjct: 275 FVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCNE 315

[Close]

BLAST of Carg16279 vs. NCBI nr
Match: XP_022145403.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 188.3 bits (477), Expect = 2.3e-44
Identity = 116/229 (50.66%), Postives = 139/229 (60.70%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQIN 61
           VM LDLSS+N V NFVS FE L+LPLNLLIN+AGRFCYE AISEDG+EMTF TN   Q  
Sbjct: 66  VMELDLSSLNSVRNFVSKFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYI 125

Query: 62  GNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQ----QVRLIFLCLRINGGL-------IA 121
           G         + +       ++ +++  +  +    Q R++ L   I+          + 
Sbjct: 126 GE-------QILFSGHFLLTKLLLNKMIETAKLTGIQGRIVNLTSNIHSWFSGDIFEYLG 185

Query: 122 ILILTRSREYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDRE 181
            +  + SREYDATRAY          TVQLAHRLQE EANVTVNCVHPGVV+TKLNRDRE
Sbjct: 186 RISRSNSREYDATRAYALSKLANVLHTVQLAHRLQETEANVTVNCVHPGVVRTKLNRDRE 245

Query: 182 GFVI------------------GAATSCKVAANRSVDNVNGKYFADCND 192
           GFV                   GAATSC VA NR+V+NVNGKYFADCN+
Sbjct: 246 GFVTDLIFYMASKTKLLKTIPQGAATSCYVATNRNVENVNGKYFADCNE 287

[Close]

BLAST of Carg16279 vs. TAIR10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 136.0 bits (341), Expect = 2.5e-32
Identity = 94/224 (41.96%), Postives = 128/224 (57.14%), Query Frame = 0


Query: 1   MVMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN-LSSQ 60
           +VM LDLSS+  V NFV++FE L LPLNLLIN+AG+  +E AISEDG+EMTFATN L   
Sbjct: 88  VVMKLDLSSIASVRNFVADFESLDLPLNLLINNAGKLAHEHAISEDGIEMTFATNYLGHF 147

Query: 61  INGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILI-----L 120
           +  N   N                 +  +++   Q R++ +   I+G     LI     +
Sbjct: 148 LLTNLLLNK---------------MIQTAEETGVQGRIVNVTSGIHGWFSGDLIEYLRLI 207

Query: 121 TRSR-EYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFV 180
           ++ + ++DATRAY          T +L+ RLQ++ ANVTVNCVHPGVV+T+L RDREG +
Sbjct: 208 SQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPGVVRTRLTRDREGLL 267

Query: 181 I----------------GAATSCKVAANRSVDNVNGKYFADCND 192
                             AAT+C VA N  + NV+GKYF DCN+
Sbjct: 268 TDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNE 296

[Close]

BLAST of Carg16279 vs. TAIR10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 134.0 bits (336), Expect = 9.5e-32
Identity = 86/221 (38.91%), Postives = 123/221 (55.66%), Query Frame = 0


Query: 1   MVMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQI 60
           +VM LDLSS+  V  FV +FE L+LPLN+LIN+AG++ ++ A+SEDG+EMTFATN     
Sbjct: 88  IVMHLDLSSLTSVRRFVDDFESLNLPLNILINNAGKYAHKHALSEDGVEMTFATNYLGHF 147

Query: 61  NGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLR--INGGLIAIL--ILTR 120
                          + + ++ I  +     + ++  +   +    +G ++  L  I   
Sbjct: 148 ------------LLTKLLLKKMIETAAQTGVQGRIVNVTSVVHSWFSGDMLQYLADISRN 207

Query: 121 SREYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVI-- 180
           +R YDATRAY          TV+L+  L +M+ANVT NCVHPG+VKT+L RDREG V   
Sbjct: 208 NRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPGIVKTRLTRDREGVVTDL 267

Query: 181 --------------GAATSCKVAANRSVDNVNGKYFADCND 192
                          AAT+C VA +  + NV GKYF+DCN+
Sbjct: 268 VFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNE 296

[Close]

BLAST of Carg16279 vs. TAIR10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 90.1 bits (222), Expect = 1.6e-18
Identity = 73/214 (34.11%), Postives = 105/214 (49.07%), Query Frame = 0


Query: 5   LDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN-LSSQINGN 64
           +DLSS++ V  F S F    LPLN+LIN+AG F      SE+ +E+TFATN L   +   
Sbjct: 94  IDLSSLSSVARFCSQFLSQDLPLNILINNAGVFSPNLEFSEEKIELTFATNFLGHYLLTE 153

Query: 65  PRPNSECDVQYPQPVFRRRIRMS----RSDKPE--QQVRLIFLCLRINGGLIAILILTRS 124
                  D      +  R I +S       KP+     +L+    R NG          +
Sbjct: 154 MLIEKMIDTAEKSGIEGRIINLSSVIHNWVKPDCFSFPKLLHPISRYNG----------T 213

Query: 125 REYDATRAYTV----QLAHRLQEMEANVTVNCVHPGVVKTKLNRDREG------FVI--- 184
           R Y  ++  T+     L+ +L++  ANVT+N VHPG+VKT + R  +G      F+I   
Sbjct: 214 RAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGIVKTGIIRAHKGLFTDSLFLIASK 273

Query: 185 -------GAATSCKVAANRSVDNVNGKYFADCND 192
                  GAAT+C VA +     ++GKYFADCN+
Sbjct: 274 LLKSISQGAATTCYVALSNETKGLSGKYFADCNE 297

[Close]

BLAST of Carg16279 vs. TAIR10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 83.2 bits (204), Expect = 1.9e-16
Identity = 69/211 (32.70%), Postives = 102/211 (48.34%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN-LSSQI 61
           ++ LDLSS+  +  FV  F  LHLPLNLLIN+AG       +SEDG+E+ FATN +   +
Sbjct: 88  LLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFCPYQLSEDGIELQFATNHIGHFL 147

Query: 62  NGNPRPNSECDVQYPQPVFRRRIRMSR-SDKPEQQVRLIFLCLRINGGLIAILILTRSRE 121
             N   ++  +      V  R + +S  +     Q  + F        +  I   +  R 
Sbjct: 148 LTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGIQF------DSINDICSYSDKRA 207

Query: 122 YDATRAYTV----QLAHRLQEMEANVTVNCVHPGVVKTKLNRDR---------------E 181
           Y  ++   +    +L+ +LQE   N+T N VHPG++ T L +                 +
Sbjct: 208 YGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQHTALLMRFLKFFSFYLWK 267

Query: 182 GFVIGAATSCKVAANRSVDNVNGKYFADCND 192
               GAAT+C VA + SV  V GKYFADCN+
Sbjct: 268 NIPQGAATTCYVALHPSVKGVTGKYFADCNE 292

[Close]

BLAST of Carg16279 vs. TAIR10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 75.9 bits (185), Expect = 3.1e-14
Identity = 65/208 (31.25%), Postives = 98/208 (47.12%), Query Frame = 0


Query: 5   LDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN-LSSQINGN 64
           +D+SS+  V +FV  F  L++PLN+LIN+AG       ++EDG+E  FATN +   +  N
Sbjct: 91  IDVSSIKSVRSFVDQFLALNVPLNILINNAGVMFCPFKLTEDGIESQFATNHIGHFLLTN 150

Query: 65  PRPNSECDVQYPQPVFRRRIRMSR-SDKPEQQVRLIFLCLRINGGLIAILILTRSREYDA 124
              +          V  R + +S  +        + F  +    G       +  R Y  
Sbjct: 151 LLLDKMKSTARESGVQGRIVNLSSIAHTYTYSEGIKFQGINDPAG------YSERRAYGQ 210

Query: 125 TR----AYTVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVI-------------- 184
           ++     ++  L+ RLQE   N+T+N VHPG+V T L R   GF +              
Sbjct: 211 SKLSNLLHSNALSRRLQEEGVNITINSVHPGLVTTNLFR-YSGFSMKVFRAMTFLFWKNI 270

Query: 185 --GAATSCKVAANRSVDNVNGKYFADCN 191
             GAAT+C VA +  ++ V GKYF DCN
Sbjct: 271 PQGAATTCYVALHPDLEGVTGKYFGDCN 291

[Close]

BLAST of Carg16279 vs. Swiss-Prot
Match: sp|A2RVM0|TIC32_ARATH (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.1e-12
Identity = 63/220 (28.64%), Postives = 93/220 (42.27%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN-----L 61
           VM LDLSSM  V  F S ++   LPLNLLIN+AG       +S+D +E+ FATN     L
Sbjct: 84  VMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPFMLSKDNIELQFATNHLGHFL 143

Query: 62  SSQINGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILILTR 121
            +++  +   ++  + +    +        R   PE                +    +  
Sbjct: 144 LTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG---------------VRFDKIND 203

Query: 122 SREYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVI-- 181
              Y + RAY            +L  +L+E   N+T N +HPG + T L R    ++   
Sbjct: 204 KSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYFNPYLAVA 263

Query: 182 --------------GAATSCKVAANRSVDNVNGKYFADCN 191
                         GAAT+C VA N  V  V+G+YF D N
Sbjct: 264 VGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSN 288

[Close]

BLAST of Carg16279 vs. Swiss-Prot
Match: sp|Q6RVV4|TIC32_PEA (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.2e-09
Identity = 65/211 (30.81%), Postives = 91/211 (43.13%), Query Frame = 0


Query: 5   LDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQINGNP 64
           LDLSS++ V  F S F     PLN+LIN+AG       +S+D +E+ FATN    I    
Sbjct: 88  LDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLSKDNIELQFATN---HIGHFL 147

Query: 65  RPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILILTRSREYDATR 124
             N   D        ++  R S+ +     V           G+    I  +S  Y+  R
Sbjct: 148 LTNLLLD------TMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKINDQS-SYNNWR 207

Query: 125 AY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDRE---------GFVI- 184
           AY            QL   L+E   N+T N +HPG + T L R            G ++ 
Sbjct: 208 AYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNSAVNGLINVIGKLVL 267

Query: 185 -----GAATSCKVAANRSVDNVNGKYFADCN 191
                GAAT+C VA +  V  V+G+YF+D N
Sbjct: 268 KNVQQGAATTCYVALHPQVKGVSGEYFSDSN 288

[Close]

BLAST of Carg16279 vs. Swiss-Prot
Match: sp|Q9ERI6|RDH14_MOUSE (Retinol dehydrogenase 14 OS=Mus musculus OX=10090 GN=Rdh14 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.7e-07
Identity = 60/215 (27.91%), Postives = 91/215 (42.33%), Query Frame = 0


Query: 1   MVMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN----- 60
           +V  LDL+S+  V  F          L++LIN+AG F      +EDG EM F  N     
Sbjct: 108 VVKELDLASLRSVRAFCQELLQEEPRLDVLINNAGVFHCPYTKTEDGFEMQFGVNHLGHF 167

Query: 61  -LSSQINGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILIL 120
            L++ + G  + ++   +        +   ++  D   +Q      C             
Sbjct: 168 LLTNLLLGLLKSSAPSRIVVVSSKLYKYGEINFEDLNSEQSYNKSFCY------------ 227

Query: 121 TRSREYDATRAYTVQLAHRLQEMEANVTVNCVHPGVVKTKLNRD-------REGFVI--- 180
             SR   A   +T +LA RL+    NVTVN +HPG+V+T L R        R  F +   
Sbjct: 228 --SRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLARPLFNLVSW 287

Query: 181 --------GAATSCKVAANRSVDNVNGKYFADCND 192
                   GA TS  +A +  V+ V+G+YF DC +
Sbjct: 288 AFFKTPLEGAQTSIYLACSPDVEGVSGRYFGDCKE 306

[Close]

BLAST of Carg16279 vs. Swiss-Prot
Match: sp|Q9HBH5|RDH14_HUMAN (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.9e-07
Identity = 59/215 (27.44%), Postives = 91/215 (42.33%), Query Frame = 0


Query: 1   MVMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATN----- 60
           +V  LDL+S+  V  F          L++LIN+AG F      +EDG EM F  N     
Sbjct: 110 IVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLGHF 169

Query: 61  -LSSQINGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILIL 120
            L++ + G  + ++   +        +   ++  D   +Q      C             
Sbjct: 170 LLTNLLLGLLKSSAPSRIVVVSSKLYKYGDINFDDLNSEQSYNKSFCY------------ 229

Query: 121 TRSREYDATRAYTVQLAHRLQEMEANVTVNCVHPGVVKTKLNRD---------------- 180
             SR   A   +T +LA RL+    NVTVN +HPG+V+T L R                 
Sbjct: 230 --SRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPLFNLVSW 289

Query: 181 --REGFVIGAATSCKVAANRSVDNVNGKYFADCND 192
              +  V GA TS  +A++  V+ V+G+YF DC +
Sbjct: 290 AFFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKE 308

[Close]

BLAST of Carg16279 vs. Swiss-Prot
Match: sp|Q8CEE7|RDH13_MOUSE (Retinol dehydrogenase 13 OS=Mus musculus OX=10090 GN=Rdh13 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 7.3e-05
Identity = 52/212 (24.53%), Postives = 85/212 (40.09%), Query Frame = 0


Query: 5   LDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQINGNP 64
           LDL+S+  +  F          +++L+N+A         +EDG EM F  N         
Sbjct: 96  LDLASLKSIREFARKVIKEEERVDILVNNAAVMRCPHWTTEDGFEMQFGVNYLGHF---- 155

Query: 65  RPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLRINGGLIAILILTRSREYDATR 124
                        +    +   ++  P + + L  L   + G +    +  + ++YD   
Sbjct: 156 ------------LLTNLLLDKLKASAPSRIINLSSLA-HVAGHIDFEDLNWQMKKYDTKA 215

Query: 125 AY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDR-------EGFVIG-- 184
           AY          T +L+HRLQ   + VTVN +HPGV +T+L R          GF++G  
Sbjct: 216 AYCQSKLAVVLFTKELSHRLQ--GSGVTVNALHPGVARTELGRHTGMHNSAFSGFMLGPF 275

Query: 185 -----------AATSCKVAANRSVDNVNGKYF 187
                      A  S  +A    ++NV+GKYF
Sbjct: 276 FWLLFKSPQLAAQPSTYLAVAEELENVSGKYF 288

[Close]

BLAST of Carg16279 vs. TrEMBL
Match: tr|A0A0A0L3R1|A0A0A0L3R1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G003470 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-53
Identity = 130/222 (58.56%), Postives = 154/222 (69.37%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQIN 61
           VMVLDLSS+N V NFVS FE L+LPLNLL++ +     E ++ +D        + + +I+
Sbjct: 93  VMVLDLSSLNSVMNFVSEFESLNLPLNLLMSFS---LDETSVEQD--------DRNGRID 152

Query: 62  GNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQ---QVRLIFLCLRINGG-LIAILILTRS 121
           GNPRPNSECDVQ+PQ VFRRRIR+S+ DKPEQ   QVRLI L L ING  +I ILI+ +S
Sbjct: 153 GNPRPNSECDVQHPQLVFRRRIRISQPDKPEQQQVQVRLILLSLCINGAPIIVILIIIQS 212

Query: 122 REYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFV---- 181
           REYDATRAY          TVQLAHRLQEM+ANVTVNCVHPGVV+T LNRDREGF+    
Sbjct: 213 REYDATRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGVVRTNLNRDREGFIKDLI 272

Query: 182 --------------IGAATSCKVAANRSVDNVNGKYFADCND 192
                           AATSC VA +R V+NVNGKYFADCN+
Sbjct: 273 FFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKYFADCNE 303

[Close]

BLAST of Carg16279 vs. TrEMBL
Match: tr|A0A1S3BLF4|A0A1S3BLF4_CUCME (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491110 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.2e-40
Identity = 109/226 (48.23%), Postives = 132/226 (58.41%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQIN 61
           VMVLDLSS+N V NFVS FE L+LPLNLLIN+AGRFCYE A SEDG+EMTFATN      
Sbjct: 93  VMVLDLSSLNSVMNFVSEFEALNLPLNLLINNAGRFCYEHATSEDGIEMTFATNYLGHF- 152

Query: 62  GNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLR--INGGLIAIL------I 121
                         + +  + I  ++S   + ++  +   +    +G +   L       
Sbjct: 153 -----------LLTKLLLNKMIETAKSTGIQGRIVNVTSNIHSWFSGDIFEYLSQISRNY 212

Query: 122 LTRSREYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFV 181
              +REYDATRAY          TVQLAHRLQEM+ANVTVNCVHPGVV+T LNRDREGF+
Sbjct: 213 NNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGVVRTNLNRDREGFL 272

Query: 182 ------------------IGAATSCKVAANRSVDNVNGKYFADCND 192
                               AATSC VA +R V+NVNGKYF DCN+
Sbjct: 273 KDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKYFEDCNE 306

[Close]

BLAST of Carg16279 vs. TrEMBL
Match: tr|A0A072TUU0|A0A072TUU0_MEDTR (NAD(P)-binding rossmann-fold protein OS=Medicago truncatula OX=3880 GN=25502242 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.8e-32
Identity = 94/221 (42.53%), Postives = 126/221 (57.01%), Query Frame = 0


Query: 1   MVMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQI 60
           +VM LDLSS+N VTNFV+ F  +  PLNLLIN+AG+F  E AISEDG+EMTFATN     
Sbjct: 89  IVMALDLSSVNSVTNFVTRFHSIGFPLNLLINNAGKFAEEHAISEDGVEMTFATNYLGHF 148

Query: 61  NGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLR--INGGLIAIL--ILTR 120
                          + + ++ +  ++    E ++  +   +     G +I+ L  I   
Sbjct: 149 ------------VLTKMLMKKMVETAKETGIEGRIVNVSSAIHGWFTGDVISYLAHICRN 208

Query: 121 SREYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVI-- 180
             EYDATRAY          T +LA RL+EM+ANVTVNCVHPGVV+T+L R+REGF+   
Sbjct: 209 KSEYDATRAYALSKLANVFHTNELARRLKEMDANVTVNCVHPGVVRTRLTREREGFLTDL 268

Query: 181 --------------GAATSCKVAANRSVDNVNGKYFADCND 192
                          AAT+C VA +  + NV+GKYFADCN+
Sbjct: 269 VFFLASKLLKTIPQAAATTCYVATHPRLVNVSGKYFADCNE 297

[Close]

BLAST of Carg16279 vs. TrEMBL
Match: tr|A0A124SCZ2|A0A124SCZ2_CYNCS (Glucose/ribitol dehydrogenase OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_002640 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.8e-32
Identity = 97/221 (43.89%), Postives = 130/221 (58.82%), Query Frame = 0


Query: 1   MVMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQI 60
           +VM LDLSS+  V +FVS+FE L+LPLNLLIN+AG+F Y+  ISEDG+EMTFATN     
Sbjct: 89  LVMPLDLSSLKSVRSFVSDFEALNLPLNLLINNAGKFSYDHGISEDGIEMTFATNYLGHF 148

Query: 61  NGNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLR--INGGLIAIL-ILTRS 120
                          + + ++ I  +++   + ++  +   +    +G LI  L  +TR 
Sbjct: 149 ------------LLTKLLLKKMIATAKTTGIQGRIVNVSSGIHTWFSGDLIRSLGQITRD 208

Query: 121 R-EYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVI-- 180
           +  YDATRAY          T +LA RLQ+MEANVTVNCVHPGVV+T L RDREGF+   
Sbjct: 209 KSHYDATRAYAISKLANVLHTKELARRLQQMEANVTVNCVHPGVVRTGLTRDREGFITDF 268

Query: 181 --------------GAATSCKVAANRSVDNVNGKYFADCND 192
                          AAT+C VA +  + NV+GKYFADCN+
Sbjct: 269 VFFLTSKFLKTIPQAAATTCYVATHPELRNVSGKYFADCNE 297

[Close]

BLAST of Carg16279 vs. TrEMBL
Match: tr|A0A251UF80|A0A251UF80_HELAN (Putative NAD(P)-binding Rossmann-fold superfamily protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr06g0166591 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.3e-32
Identity = 97/220 (44.09%), Postives = 127/220 (57.73%), Query Frame = 0


Query: 2   VMVLDLSSMNFVTNFVSNFEFLHLPLNLLINSAGRFCYERAISEDGMEMTFATNLSSQIN 61
           VM LDLSSM  V  FVS FE L+LPLNLLIN+AG+F YE  ISEDG+EMTFATN      
Sbjct: 90  VMHLDLSSMKSVRQFVSEFEALNLPLNLLINNAGKFSYEHGISEDGIEMTFATNYLGHF- 149

Query: 62  GNPRPNSECDVQYPQPVFRRRIRMSRSDKPEQQVRLIFLCLR--INGGLIAIL-ILTRSR 121
                         + + ++ I  +++   + ++  +   +    +G LI  L  +TR +
Sbjct: 150 -----------LLTKLLLKKMITTAKTTGVQGRIVNVTSGIHTWFSGDLIRSLGQITRDK 209

Query: 122 -EYDATRAY----------TVQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVI--- 181
             YDATRAY          T +LA RLQ MEANVTVNCVHPG+V+T+L RDREGF+    
Sbjct: 210 SHYDATRAYAISKLANVLHTKELASRLQMMEANVTVNCVHPGIVRTRLTRDREGFITDLV 269

Query: 182 -------------GAATSCKVAANRSVDNVNGKYFADCND 192
                         AAT+C VA + ++ N +GKYFADCN+
Sbjct: 270 FFLTSKFLKTIPQAAATTCYVATHPNLKNTSGKYFADCNE 297

The following BLAST results are available for this feature:

BLAST of Carg16279 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952610.1	8.9e-60	68.39	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata]	[more]
KGN55679.1	1.6e-53	58.56	hypothetical protein Csa_3G003470 [Cucumis sativus]	[more]
XP_022145404.1	1.4e-44	53.21	short-chain dehydrogenase TIC 32, chloroplastic isoform X4 [Momordica charantia]	[more]
XP_022145401.1	2.3e-44	51.75	short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Momordica charantia]	[more]
XP_022145403.1	2.3e-44	50.66	short-chain dehydrogenase TIC 32, chloroplastic isoform X3 [Momordica charantia]	[more]

BLAST of Carg16279 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G24050.1	2.5e-32	41.96	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	9.5e-32	38.91	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	1.6e-18	34.11	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	1.9e-16	32.70	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G37540.1	3.1e-14	31.25	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of Carg16279 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|A2RVM0\|TIC32_ARATH	6.1e-12	28.64	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
sp\|Q6RVV4\|TIC32_PEA	2.2e-09	30.81	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
sp\|Q9ERI6\|RDH14_MOUSE	2.7e-07	27.91	Retinol dehydrogenase 14 OS=Mus musculus OX=10090 GN=Rdh14 PE=1 SV=1	[more]
sp\|Q9HBH5\|RDH14_HUMAN	5.9e-07	27.44	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]
sp\|Q8CEE7\|RDH13_MOUSE	7.3e-05	24.53	Retinol dehydrogenase 13 OS=Mus musculus OX=10090 GN=Rdh13 PE=1 SV=1	[more]

BLAST of Carg16279 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L3R1\|A0A0A0L3R1_CUCSA	1.1e-53	58.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G003470 PE=4 SV=1	[more]
tr\|A0A1S3BLF4\|A0A1S3BLF4_CUCME	5.2e-40	48.23	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=...	[more]
tr\|A0A072TUU0\|A0A072TUU0_MEDTR	1.8e-32	42.53	NAD(P)-binding rossmann-fold protein OS=Medicago truncatula OX=3880 GN=25502242 ...	[more]
tr\|A0A124SCZ2\|A0A124SCZ2_CYNCS	1.8e-32	43.89	Glucose/ribitol dehydrogenase OS=Cynara cardunculus var. scolymus OX=59895 GN=Cc...	[more]
tr\|A0A251UF80\|A0A251UF80_HELAN	2.3e-32	44.09	Putative NAD(P)-binding Rossmann-fold superfamily protein OS=Helianthus annuus O...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR036291	NAD(P)-bd_dom_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16279-RA	Carg16279-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.40.50.720		coord: 1..62 e-value: 4.4E-6 score: 28.0
None	No IPR available	GENE3D	G3DSA:3.40.50.720		coord: 113..191 e-value: 1.0E-10 score: 42.9
None	No IPR available	PANTHER	PTHR24320:SF114	SUBFAMILY NOT NAMED	coord: 1..56
None	No IPR available	PANTHER	PTHR24320	FAMILY NOT NAMED	coord: 115..191
None	No IPR available	PANTHER	PTHR24320:SF114	SUBFAMILY NOT NAMED	coord: 115..191
None	No IPR available	PANTHER	PTHR24320	FAMILY NOT NAMED	coord: 1..56
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 116..158 coord: 2..64

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg16279	Cla008879	Watermelon (97103) v1	carwmB0678
Carg16279	Csa3G003470	Cucumber (Chinese Long) v2	carcuB0701
Carg16279	ClCG10G000880	Watermelon (Charleston Gray)	carwcgB0655
Carg16279	CmaCh06G005930	Cucurbita maxima (Rimu)	carcmaB0957
Carg16279	CmoCh06G005980	Cucurbita moschata (Rifu)	carcmoB0933
Carg16279	CsGy3G000850	Cucumber (Gy14) v2	carcgybB0677

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg16279	Cucumber (Gy14) v1	carcgyB0759
Carg16279	Cucurbita maxima (Rimu)	carcmaB0956
Carg16279	Cucurbita moschata (Rifu)	carcmoB0932
Carg16279	Cucurbita pepo (Zucchini)	carcpeB0955
Carg16279	Cucurbita pepo (Zucchini)	carcpeB0957
Carg16279	Wild cucumber (PI 183967)	carcpiB0713
Carg16279	Cucumber (Chinese Long) v3	carcucB0715
Carg16279	Bottle gourd (USVL1VR-Ls)	carlsiB621
Carg16279	Melon (DHL92) v3.5.1	carmeB0681
Carg16279	Melon (DHL92) v3.6.1	carmedB0693
Carg16279	Watermelon (97103) v2	carwmbB0705
Carg16279	Wax gourd	carwgoB0798
Carg16279	Silver-seed gourd	carcarB442