Carg13552 (gene) Silver-seed gourd

NameCarg13552
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionIndole-3-acetic acid-amido synthetase GH3.3, putative
LocationCucurbita_argyrosperma_scaffold_053 : 213510 .. 216145 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCGTATCGGAATTCTTAACCAGGTGAGTGTAAACAAATCTATTGTAGTTATTCTTGTTAGAGACTACGACTCTTCACAATCGTTTGATATTGTCTACTTTCAGTATAAGCTCTCATAATTTTGAATAAAGTACACCATAGAGCCTCCCCTAAGGCTTACGGAGCCCTCGAACAACCTCCCCTTAATCGAGGCTCAACTCTTTGTTTGGAGCTTTTGAACAAAGTAAACCCTTCGTTCAATACTTTAGTCACTTTTGACTACACTTTCGAGGCTCACAATTTCTTTGTTCGACATTTGAGAATTCTATTGATATGGCTAAGTTAAGGGCATGGCTCTAATACCAGGATAGGAACCATGACTCTCCACAATGGTATGATATTGTCCATTTTGAGCATAAGCTCTCGTGGCTTTGCTTTTGGTTTCTCCAAAAGACATCATACTTAGCCGATGATCAACCCCTCAATTAGCCGTTGTGGTACTCCTCTCCCAATTGTGTTTGAACTCAATTGTTGTTTGTGTTTTTCTATTCTAGTTCCGGAACATCGGGAGGGGAGAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGCGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTATCATACCTAGGCTGTGGCCAAATACAAAGTATATTGATGTTATTGTGACAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGGTATGTTTTTGTTGAGACATAGAGAATCTATGGGTCTGCTGCCGATTTATAGCTTATTCAATTGTTATAACTGTTAGACACAATTGTTTAGACCCTACGGAGCCTGTAGACTCAATTGTTTAGACTCTACGGAGTTGGTGTCTATGACAGTGTGTATAGTTTTCCTGTATATGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCTGGTTGATTAAGACTCCTACATGAATGAATAATATTTGGTTCAGGTATCTAAGATAACCTATCTTATTTGATATTGCAGGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACACCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATTATAG

mRNA sequence

ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCGTATCGGAATTCTTAACCAGTTCCGGAACATCGGGAGGGGAGAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGCGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTATCATACCTAGGCTGTGGCCAAATACAAAGTATATTGATGTTATTGTGACAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACACCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATTATAG

Coding sequence (CDS)

ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCGTATCGGAATTCTTAACCAGTTCCGGAACATCGGGAGGGGAGAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGCGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTATCATACCTAGGCTGTGGCCAAATACAAAGTATATTGATGTTATTGTGACAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACACCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATTATAG

Protein sequence

MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKL
BLAST of Carg13552 vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 568/570 (99.65%), Postives = 569/570 (99.82%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           TGTINPRI DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG
Sbjct: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420
           RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL
Sbjct: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420

Query: 421 SIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480
           SID+DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI
Sbjct: 421 SIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480

Query: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540
           PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY
Sbjct: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540

Query: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           KTPRCVKHEPILELLNSYVVSNYFSPRCPK
Sbjct: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 570

BLAST of Carg13552 vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 561/570 (98.42%), Postives = 568/570 (99.65%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MP +DEGA+LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 8   MPERDEGAILQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 67

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS
Sbjct: 68  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 127

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLN+GKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 128 LLMPVMNQFIPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 187

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMY QFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 188 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 247

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG
Sbjct: 248 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 307

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 308 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 367

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420
           R+MSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL
Sbjct: 368 REMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 427

Query: 421 SIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480
           SID+DKVDEVELQNAVMEASKNL+PYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI
Sbjct: 428 SIDSDKVDEVELQNAVMEASKNLVPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 487

Query: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540
           PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLE+KIIENGTFDKLMDYAINHGSSINQY
Sbjct: 488 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGSSINQY 547

Query: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           KTPRCVKHEPILELLNSYVVSNYFSPRCPK
Sbjct: 548 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 577

BLAST of Carg13552 vs. NCBI nr
Match: XP_022970970.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 558/570 (97.89%), Postives = 565/570 (99.12%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP+SEFLTSSGTSGGERKLMPTT+EELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMY QFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGII RLWPNTKY+DVIVTG
Sbjct: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLD YSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420
           RKMSEELVDLVDVELGKEYEL+ITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL
Sbjct: 361 RKMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420

Query: 421 SIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480
           SID+DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI
Sbjct: 421 SIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480

Query: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540
           PD VFENCCLTIEESLNSVYRQGRVSDKSIGPLE+KIIENGTFDKLMDYAINHGSSINQY
Sbjct: 481 PDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGSSINQY 540

Query: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           KTPRCVK EPILELLNSYVVSNYFSP+CPK
Sbjct: 541 KTPRCVKREPILELLNSYVVSNYFSPKCPK 570

BLAST of Carg13552 vs. NCBI nr
Match: XP_022140599.1 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia])

HSP 1 Score: 986.1 bits (2548), Expect = 5.0e-284
Identity = 481/581 (82.79%), Postives = 521/581 (89.67%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL + GLDG MD   FK  +PVVSY+++KP
Sbjct: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTMPVVSYDDIKP 86

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           Y+DRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPTTEEEL+R+S LYSLLMPVMNQF
Sbjct: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           IPGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           TDSYQSMY QFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI+
Sbjct: 207 TDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DPLVRQSV+NILKPDP LADLIE ECSKGTW GII RLW NTKYIDVIVTGTMSQYIPTL
Sbjct: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 369
           DYYSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386

Query: 370 -------RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                  +K  +ELVDLVDV+LGKEYELVITTY+GLYRYRVGDILRVSGFKN APQFNFV
Sbjct: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFV 446

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNVILSID+DKVDEVELQNAVMEASKNLIPY   ++EYTSYADT T+PGHYVIYWEIS
Sbjct: 447 CRKNVILSIDSDKVDEVELQNAVMEASKNLIPYGACVVEYTSYADTVTIPGHYVIYWEIS 506

Query: 490 QKE-----TNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMD 549
            KE      N IP  VFE CCL++EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLMD
Sbjct: 507 LKEATANNNNVIPGSVFEKCCLSMEESLNSVYRQGRVSDKSIGPLEMKIVETGTFDKLMD 566

Query: 550 YAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
           YA+NHG+SINQYKTPRCVKH PILELLNSYVV NYFSP+CP
Sbjct: 567 YALNHGASINQYKTPRCVKHAPILELLNSYVVDNYFSPKCP 606

BLAST of Carg13552 vs. NCBI nr
Match: XP_023901431.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Quercus suber] >POE49631.1 indole-3-acetic acid-amido synthetase gh3.6 [Quercus suber])

HSP 1 Score: 911.0 bits (2353), Expect = 2.0e-261
Identity = 437/585 (74.70%), Postives = 507/585 (86.67%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           + GK++ A LQ IE++T++AD+VQRRVL  ILSR+AHVEYLQ+ GL+GC D+  FK +VP
Sbjct: 22  LDGKNKKA-LQFIEDVTSNADEVQRRVLAGILSRSAHVEYLQRHGLNGCTDQETFKKVVP 81

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VV+YE+LKP IDRIANGDASPILCS+P+SEFLTSSGTSGGERKLMPT EEEL+RRS LYS
Sbjct: 82  VVTYEDLKPEIDRIANGDASPILCSQPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 141

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQ +PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKSSHF DRPYD YT+Y
Sbjct: 142 LLMPVMNQCVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFMDRPYDPYTNY 201

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPN+TILC DSYQSMY Q LCGL  + QVLRVG+VFASGFIRA+KFL+ +W+LL NDIR
Sbjct: 202 TSPNETILCPDSYQSMYSQLLCGLYQNTQVLRVGAVFASGFIRALKFLQMNWLLLCNDIR 261

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           +GT++P+I+D  VR++VM IL+P+P LAD IE EC K +W+GII RLWPNTKYIDVIVTG
Sbjct: 262 SGTLDPKITDSSVREAVMKILEPNPRLADFIEDECGKDSWSGIITRLWPNTKYIDVIVTG 321

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC P+EVSYTLIPTM Y+EFLP++
Sbjct: 322 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCKPSEVSYTLIPTMCYYEFLPVN 381

Query: 361 RKM----------------SEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFK 420
           RK                 +++LVDLVDV+LG+EYELV+TTY+GLYRYRVGDILRV+GFK
Sbjct: 382 RKSGFNESFSKPVSLNDKENQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDILRVAGFK 441

Query: 421 NKAPQFNFVCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPG 480
           NKAPQFNFVCRKNV+LSID+DK DEVELQNAV  A+ NL+ ++ SL EYTSYADT TVPG
Sbjct: 442 NKAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNAANNLLSFDASLTEYTSYADTSTVPG 501

Query: 481 HYVIYWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFD 540
           HYV+YWEIS      IP  VFE+CCLT+EES NSVYRQGRVSDKSIGPLEIKI+E+GTFD
Sbjct: 502 HYVLYWEISLSRATPIPPSVFEDCCLTVEESFNSVYRQGRVSDKSIGPLEIKIVESGTFD 561

Query: 541 KLMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
            LMDYA+  G+SINQYK PRCVK+ PILELLNS VV+NYFSP+CP
Sbjct: 562 SLMDYAVGQGASINQYKAPRCVKYAPILELLNSKVVANYFSPKCP 605

BLAST of Carg13552 vs. TAIR10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 889.4 bits (2297), Expect = 1.2e-258
Identity = 428/577 (74.18%), Postives = 492/577 (85.27%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++TT+AD VQRRVL EILSRNA VEYL++ GL+G  DR  FK+I+PVV+YE+++P
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD S +LCS P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPNQTILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  LA DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++V  ILKPDP LAD +E EC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+L V+GFKN APQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIK++E+GTFDKLMDYAI+ 
Sbjct: 505 LNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552 vs. TAIR10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 873.6 bits (2256), Expect = 6.6e-254
Identity = 419/577 (72.62%), Postives = 485/577 (84.06%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IEE+T++ADQVQ       L+RNA VEYL++  L+G  DR  FKNI+PV++YE+++P
Sbjct: 25  LQLIEELTSNADQVQXXXXXXXLTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPIL S+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL  GKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  Q+VLRVG+VFASGFIRAIKFLE HW+ L  DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  ILKP P LAD +EFEC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP+MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GL RYRVGD+LRV+GFKNKAPQF+F+
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCL +EES N+VYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 505 LDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552 vs. TAIR10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 747.3 bits (1928), Expect = 7.1e-216
Identity = 365/572 (63.81%), Postives = 449/572 (78.50%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IEE+T + D VQ +VL EIL+RN++ EYL++  LDG +DR  FK+ VPVV+YE+LKP
Sbjct: 24  LKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYEDLKP 83

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I RI+NGD SPIL S P++EFLTSSGTS GERKLMPT EE+L RR  LYSLLMPVMN +
Sbjct: 84  EIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPVMNLY 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSES T GGLPARPVLTSYYKS HFK RPYD Y  YTSPN+ ILC
Sbjct: 144 VPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNEAILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DS QSMY Q LCGL +  +VLR+G+VFASG +RAI FL+ +W  LA DI TGT++ RI 
Sbjct: 204 SDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLSSRIF 263

Query: 250 DPLVRQSVMNIL-KPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPT 309
           DP ++  +  IL KPD  LA+ +   CS+  W GII ++WPNTKY+DVIVTG M+QYIPT
Sbjct: 264 DPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMAQYIPT 323

Query: 310 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP--------LD 369
           L+YYS GLP+ CTMYASSE YFG+NL P+C P+EVSYT++P MAYFEFLP         +
Sbjct: 324 LEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDGAAE 383

Query: 370 RKMSE-ELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVI 429
             + E  LV+L +VE+GKEYELVITTY+GLYRYRVGDILRV+GF N APQF F+ RKNV+
Sbjct: 384 ASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRKNVL 443

Query: 430 LSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWE-ISQKETN 489
           LS+++DK DE ELQ AV  AS+      T ++EYTSYA+T T+PGHYVIYWE + + ++N
Sbjct: 444 LSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELLGRDQSN 503

Query: 490 AI-PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSI 549
           A+  + V   CCL +EESLNSVYRQ RV+DKSIGPLEI+++ NGTF++LMDYAI+ G+SI
Sbjct: 504 ALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAISRGASI 563

Query: 550 NQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
           NQYK PRCV   PI+ELL+S VVS +FSP  P
Sbjct: 564 NQYKVPRCVSFTPIMELLDSRVVSAHFSPSLP 595

BLAST of Carg13552 vs. TAIR10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 741.5 bits (1913), Expect = 3.9e-214
Identity = 362/562 (64.41%), Postives = 436/562 (77.58%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IEE+T +AD VQ  +L EIL+RNA  EYL++  L G  DR  FK  +PV++YE+L+P
Sbjct: 23  LRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVITYEDLQP 82

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I RIA+GD SPIL + P+SEFLTSSGTS GERKLMPT +EEL RR  LYSLLMPVMN +
Sbjct: 83  EIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLY 142

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLFVKSE+ TPGGLPARPVLTSYYKS HF+ RPYD Y  YTSPN+ ILC
Sbjct: 143 VPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSPNEAILC 202

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DS+QSMY Q LCGL     VLRVG+VFASG +RAI+FL+ HW   A+DI  G ++  I+
Sbjct: 203 PDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGCLDSEIT 262

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP +RQ +  ILKPDPVLA+ I  EC    W  II R+WPNTKY+DVIVTG M+QYIPTL
Sbjct: 263 DPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMAQYIPTL 322

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRKMSEELVD 369
           +YYS GLP+ CTMYASSECYFG+NLNP+  P+EVSYT++P MAYFEF+PL      + V+
Sbjct: 323 EYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG---GTKAVE 382

Query: 370 LVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDTDKVDE 429
           LVDV +GKEYELV+TTY+GL RYRVGDILRV+GF N APQF+FV RKNV+LSID+DK DE
Sbjct: 383 LVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDE 442

Query: 430 VELQNAVMEASKNL-IPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPD-LVFEN 489
            ELQ AV  AS  L     + + EYTSYADT T+PGHYV+YWE+  ++    P       
Sbjct: 443 SELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDGARQPSHETLTR 502

Query: 490 CCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVK 549
           CCL +EESLNSVYRQ RV+D S+GPLEI+++ NGTF++LMDYAI+ G+SINQYK PRCV 
Sbjct: 503 CCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVN 562

Query: 550 HEPILELLNSYVVSNYFSPRCP 570
             PI+ELL+S VVS +FSP  P
Sbjct: 563 FTPIVELLDSRVVSAHFSPSLP 581

BLAST of Carg13552 vs. TAIR10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 740.3 bits (1910), Expect = 8.7e-214
Identity = 368/573 (64.22%), Postives = 447/573 (78.01%), Query Frame = 0

Query: 2   PGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPV 61
           P   +   L+ IEE+T + D VQ++V+ EILSRN+  EYL++ GL G  DR  FK  VPV
Sbjct: 15  PSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPV 74

Query: 62  VSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSL 121
           V Y++LKP I RIANGD S IL S P++EFLTSSGTS GERKLMPT +E++ RR  LYSL
Sbjct: 75  VIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSL 134

Query: 122 LMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYT 181
           LMPVMN ++PGL+KGK +YFLFVK+ES TPGGLPARPVLTSYYKS  FK RP D Y  YT
Sbjct: 135 LMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYT 194

Query: 182 SPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRT 241
           SPN+ ILC DS QSMY Q LCGL +  +VLR+G+VFASG +RAI FL+T+W  LA+DI T
Sbjct: 195 SPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDIST 254

Query: 242 GTINPRISDPLVRQSVMNIL-KPDPVLADLIEFECSK-GTWAGIIPRLWPNTKYIDVIVT 301
           GT++ RISDP +++S+  IL KPD  LAD I   C +  +W GII ++WPNTKY+DVIVT
Sbjct: 255 GTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVT 314

Query: 302 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 361
           G M+QYIP L+YYS GLP+ CTMYASSE YFG+NL P+C P+EVSYT++P MAYFEFLP 
Sbjct: 315 GAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPH 374

Query: 362 DRKMSE--ELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKN 421
               +E  ELV+L DVE+GKEYELVITTY+GL RYRVGDIL+V+GF N APQF FV RKN
Sbjct: 375 HEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRKN 434

Query: 422 VILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK-E 481
           V+LSI++DK DE ELQ+AV  AS  L    T ++EYTSYA+T T+PGHYVIYWE+  K +
Sbjct: 435 VLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKDQ 494

Query: 482 TNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSS 541
           TN   D V   CCL +EESLNSVYRQ RV+DKSIGPLEI++++NGTF++LMDYAI+ G+S
Sbjct: 495 TNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGAS 554

Query: 542 INQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
           INQYK PRCV   PI+ELL+S VVS +FSP  P
Sbjct: 555 INQYKVPRCVSFTPIMELLDSRVVSTHFSPALP 587

BLAST of Carg13552 vs. Swiss-Prot
Match: sp|Q9LSQ4|GH36_ARATH (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 2.1e-257
Identity = 428/577 (74.18%), Postives = 492/577 (85.27%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++TT+AD VQRRVL EILSRNA VEYL++ GL+G  DR  FK+I+PVV+YE+++P
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD S +LCS P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPNQTILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  LA DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++V  ILKPDP LAD +E EC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+L V+GFKN APQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIK++E+GTFDKLMDYAI+ 
Sbjct: 505 LNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552 vs. Swiss-Prot
Match: sp|O81829|GH35_ARATH (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 1.2e-252
Identity = 419/577 (72.62%), Postives = 485/577 (84.06%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IEE+T++ADQVQ       L+RNA VEYL++  L+G  DR  FKNI+PV++YE+++P
Sbjct: 25  LQLIEELTSNADQVQXXXXXXXLTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPIL S+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL  GKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  Q+VLRVG+VFASGFIRAIKFLE HW+ L  DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  ILKP P LAD +EFEC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP+MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GL RYRVGD+LRV+GFKNKAPQF+F+
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCL +EES N+VYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 505 LDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552 vs. Swiss-Prot
Match: sp|Q8LQM5|GH31_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 4.1e-221
Identity = 370/568 (65.14%), Postives = 451/568 (79.40%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRM-AFKNIVPVVSYENLK 69
           L+ IE +T +A QVQRRVL EIL++NA  EYL++ G+ G  D + AF+ +VP+V+YE L+
Sbjct: 24  LEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEGLQ 83

Query: 70  PYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQ 129
           P I RIANGD SPI   +P+SEFLTSSGTSGGERKLMPT  +E+ RRS LYSLLMPVM+Q
Sbjct: 84  PDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQ 143

Query: 130 FIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTIL 189
            + GL+KGK MY LFVK+ES TPGGL ARPVLTSYY+S  F DRP D YT YTSP++ IL
Sbjct: 144 SVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAIL 203

Query: 190 CTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRI 249
           C DSYQSMY Q LCGL     VLRVG+VFASGF+RAI FLE HW  L +DIRTG ++P I
Sbjct: 204 CVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDPEI 263

Query: 250 SDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPT 309
           +D +VR +V  +L+ DP LAD IE EC++ +W GII RLWP TKYIDVIVTGTMSQYIPT
Sbjct: 264 TDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIPT 323

Query: 310 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD------RK 369
           L++Y  GLPL CTMYASSECYFG+NLNP+C P++V+YTLIPTM Y+EFLP++        
Sbjct: 324 LEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATAEA 383

Query: 370 MSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSI 429
              +LVDLVDV+LG EYELV+TTYSGLYRYRVGD+LRV+GFKNKAP F+FV R+NV LS+
Sbjct: 384 SHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVALSV 443

Query: 430 DTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPD 489
           D+DK DE EL  AV  A ++L P+  SL+EYTSYAD  T+PGHYV++WE+    T A+P 
Sbjct: 444 DSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAGST-AVPA 503

Query: 490 LVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKT 549
            VFE CCL++EE+LNSVYRQGR  D+SIGPLEI+++  GTFDKLMDYAI+ G+SINQYK 
Sbjct: 504 SVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQYKA 563

Query: 550 PRCVKHEPILELLNSYVVSNYFSPRCPK 571
           PRCV+  P++ELL++ V   YFSP+CPK
Sbjct: 564 PRCVRPGPVVELLDARVQGKYFSPKCPK 590

BLAST of Carg13552 vs. Swiss-Prot
Match: sp|A3BLS0|GH38_ORYSI (Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=2 SV=2)

HSP 1 Score: 756.5 bits (1952), Expect = 2.1e-217
Identity = 368/567 (64.90%), Postives = 446/567 (78.66%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GLDG  DR AF+  VPVVSY++L+P
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           YI RIANGD SPIL + PVSEFLTSSGTS GERKLMPT  +EL RR  LYSLLMPVMN +
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSE+ TPGGL ARPVLTSYYKS HFK+RPYD Y +YTSP   ILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            D++QSMY Q +CGLC    VLR+G+VFASG +RAI+FL+ +W  LA+DI +G + PR++
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  IL PDP LA LI  ECSKG WAGII R+WPNTKY+DVIVTG M+QYIPTL
Sbjct: 270 DPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTL 329

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRK-----MS 369
           ++YS GLP+ CTMYASSECYFG+NL P+C+P+EVSYT++P M YFEFLP+D        +
Sbjct: 330 EFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDA 389

Query: 370 EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDT 429
            +LVDL  VE+G+EYELVITTY+GL RYRVGD+LRV+GF N APQF FV RKNV+LSI++
Sbjct: 390 TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIES 449

Query: 430 DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNA--IPD 489
           DK DE ELQ AV  AS  L P+  S++EYTS A T  +PGHYVIYWE+  K   A  +  
Sbjct: 450 DKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDA 509

Query: 490 LVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKT 549
                CCL +EE+LN+VYRQ RV+D SIGPLEI+++  GTF++LMDYAI+ G+SINQYK 
Sbjct: 510 DTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKV 569

Query: 550 PRCVKHEPILELLNSYVVSNYFSPRCP 570
           PRCV   PI+ELL+S VVS++FSP  P
Sbjct: 570 PRCVTFPPIVELLDSRVVSSHFSPALP 596

BLAST of Carg13552 vs. Swiss-Prot
Match: sp|Q0D4Z6|GH38_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=2 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.1e-217
Identity = 368/567 (64.90%), Postives = 446/567 (78.66%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GLDG  DR AF+  VPVVSY++L+P
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           YI RIANGD SPIL + PVSEFLTSSGTS GERKLMPT  +EL RR  LYSLLMPVMN +
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSE+ TPGGL ARPVLTSYYKS HFK+RPYD Y +YTSP   ILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            D++QSMY Q +CGLC    VLR+G+VFASG +RAI+FL+ +W  LA+DI +G + PR++
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  IL PDP LA LI  ECSKG WAGII R+WPNTKY+DVIVTG M+QYIPTL
Sbjct: 270 DPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTL 329

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRK-----MS 369
           ++YS GLP+ CTMYASSECYFG+NL P+C+P+EVSYT++P M YFEFLP+D        +
Sbjct: 330 EFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDA 389

Query: 370 EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDT 429
            +LVDL  VE+G+EYELVITTY+GL RYRVGD+LRV+GF N APQF FV RKNV+LSI++
Sbjct: 390 TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIES 449

Query: 430 DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNA--IPD 489
           DK DE ELQ AV  AS  L P+  S++EYTS A T  +PGHYVIYWE+  K   A  +  
Sbjct: 450 DKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDA 509

Query: 490 LVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKT 549
                CCL +EE+LN+VYRQ RV+D SIGPLEI+++  GTF++LMDYAI+ G+SINQYK 
Sbjct: 510 DTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKV 569

Query: 550 PRCVKHEPILELLNSYVVSNYFSPRCP 570
           PRCV   PI+ELL+S VVS++FSP  P
Sbjct: 570 PRCVTFPPIVELLDSRVVSSHFSPALP 596

BLAST of Carg13552 vs. TrEMBL
Match: tr|A0A2P4H061|A0A2P4H061_QUESU (Indole-3-acetic acid-amido synthetase gh3.6 OS=Quercus suber OX=58331 GN=CFP56_05848 PE=4 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 1.4e-261
Identity = 437/585 (74.70%), Postives = 507/585 (86.67%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           + GK++ A LQ IE++T++AD+VQRRVL  ILSR+AHVEYLQ+ GL+GC D+  FK +VP
Sbjct: 22  LDGKNKKA-LQFIEDVTSNADEVQRRVLAGILSRSAHVEYLQRHGLNGCTDQETFKKVVP 81

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VV+YE+LKP IDRIANGDASPILCS+P+SEFLTSSGTSGGERKLMPT EEEL+RRS LYS
Sbjct: 82  VVTYEDLKPEIDRIANGDASPILCSQPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 141

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQ +PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKSSHF DRPYD YT+Y
Sbjct: 142 LLMPVMNQCVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFMDRPYDPYTNY 201

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPN+TILC DSYQSMY Q LCGL  + QVLRVG+VFASGFIRA+KFL+ +W+LL NDIR
Sbjct: 202 TSPNETILCPDSYQSMYSQLLCGLYQNTQVLRVGAVFASGFIRALKFLQMNWLLLCNDIR 261

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           +GT++P+I+D  VR++VM IL+P+P LAD IE EC K +W+GII RLWPNTKYIDVIVTG
Sbjct: 262 SGTLDPKITDSSVREAVMKILEPNPRLADFIEDECGKDSWSGIITRLWPNTKYIDVIVTG 321

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC P+EVSYTLIPTM Y+EFLP++
Sbjct: 322 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCKPSEVSYTLIPTMCYYEFLPVN 381

Query: 361 RKM----------------SEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFK 420
           RK                 +++LVDLVDV+LG+EYELV+TTY+GLYRYRVGDILRV+GFK
Sbjct: 382 RKSGFNESFSKPVSLNDKENQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDILRVAGFK 441

Query: 421 NKAPQFNFVCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPG 480
           NKAPQFNFVCRKNV+LSID+DK DEVELQNAV  A+ NL+ ++ SL EYTSYADT TVPG
Sbjct: 442 NKAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNAANNLLSFDASLTEYTSYADTSTVPG 501

Query: 481 HYVIYWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFD 540
           HYV+YWEIS      IP  VFE+CCLT+EES NSVYRQGRVSDKSIGPLEIKI+E+GTFD
Sbjct: 502 HYVLYWEISLSRATPIPPSVFEDCCLTVEESFNSVYRQGRVSDKSIGPLEIKIVESGTFD 561

Query: 541 KLMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
            LMDYA+  G+SINQYK PRCVK+ PILELLNS VV+NYFSP+CP
Sbjct: 562 SLMDYAVGQGASINQYKAPRCVKYAPILELLNSKVVANYFSPKCP 605

BLAST of Carg13552 vs. TrEMBL
Match: tr|A0A1U7YQ53|A0A1U7YQ53_NELNU (indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC104585725 PE=4 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 3.9e-261
Identity = 435/577 (75.39%), Postives = 498/577 (86.31%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IE++TT+AD++Q+RVL EILSR+AHVEYLQ+ GL+G  DR  FK ++PVV+YE+L+ 
Sbjct: 31  LEFIEDVTTNADEIQKRVLAEILSRSAHVEYLQRHGLNGRTDRETFKKVMPVVTYEDLQS 90

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPILC  P+SEFLTSSGTS GERKLMPT EEEL+RRS LYSLLMPVM+QF
Sbjct: 91  DINRIANGDTSPILCGLPISEFLTSSGTSAGERKLMPTIEEELERRSLLYSLLMPVMDQF 150

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKS HFK RPYD YT+YTSPN+TILC
Sbjct: 151 VPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSLHFKHRPYDPYTNYTSPNETILC 210

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DSYQSMY Q LCGLC ++QVLRVG+VFASGFIRAI+FLE HW LL NDIRTG INP+I+
Sbjct: 211 PDSYQSMYSQLLCGLCQNKQVLRVGAVFASGFIRAIRFLEKHWSLLCNDIRTGAINPQIT 270

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR +V+ ILKPDP LAD IE EC++  W GII RLWPNTKYIDVIVTGTMSQYIPTL
Sbjct: 271 DPSVRDAVLRILKPDPELADFIESECTREAWQGIITRLWPNTKYIDVIVTGTMSQYIPTL 330

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP----------- 369
           DYYSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLP           
Sbjct: 331 DYYSNGLPLVCTMYASSECYFGLNLNPLCKPNEVSYTLIPTMAYFEFLPVHRNNGFTTSI 390

Query: 370 -----LDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                L+ K  ++LVDLVDV+LG+EYELV+TTY+GLYRYRVGD+LRV+GFKNKAPQFNFV
Sbjct: 391 SVPKSLNEKEQQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNKAPQFNFV 450

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQ AV +A  +L+P++ SL+EYTSYADT T+PGHYV+YWE+ 
Sbjct: 451 CRKNVVLSIDSDKTDEVELQAAVKDAINHLVPFDASLIEYTSYADTTTIPGHYVLYWELR 510

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
              T  IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 511 HDSTTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAISR 570

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS V+SNYFSP+CPK
Sbjct: 571 GASINQYKTPRCVKFAPIVELLNSRVLSNYFSPKCPK 607

BLAST of Carg13552 vs. TrEMBL
Match: tr|B9T670|B9T670_RICCO (Indole-3-acetic acid-amido synthetase GH3.6, putative OS=Ricinus communis OX=3988 GN=RCOM_0032550 PE=4 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 5.7e-260
Identity = 432/577 (74.87%), Postives = 500/577 (86.66%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++T++ D+VQ++VL EIL+RNA VEYLQ+ GL+G  DR +FK I+PV++YE+++P
Sbjct: 24  LQFIEDVTSNPDEVQKKVLEEILTRNARVEYLQKHGLNGYTDRESFKKIMPVIAYEDIQP 83

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           +I+RIANGD SPILCS+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVMNQF
Sbjct: 84  HINRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLMPVMNQF 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKSSHFKDRP+D YT+YTSPN+TILC
Sbjct: 144 VPGLEKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFKDRPFDPYTNYTSPNETILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DSYQSMY Q LCGLC    VLRVG+VFASGFIRAI+FLE HW LL NDIRTGT+NP+I+
Sbjct: 204 PDSYQSMYSQMLCGLCQRNDVLRVGAVFASGFIRAIRFLEKHWQLLCNDIRTGTVNPQIT 263

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++VM ILKPDP LAD I  ECS+ +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 264 DLSVREAVMKILKPDPKLADFIAAECSQESWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 323

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP----------- 369
           DYYSNGLPLVCTMYASSECYFGVNLNPLC P+EVSYTLIPTMAYFEFLP           
Sbjct: 324 DYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVHRNNGVTNSI 383

Query: 370 -----LDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                L+ K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+LRV+GFKNKAPQFNF+
Sbjct: 384 SMPKSLNEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNKAPQFNFI 443

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ +L EYTSYADT ++PGHYV++WEI+
Sbjct: 444 CRKNVVLSIDSDKTDEVELQNAVKNAVNHLVPFDATLAEYTSYADTTSIPGHYVLFWEIT 503

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
              +  IP  +FE+CCLTIEESLNSVYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 504 LNGSTPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 563

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VVS+YFSP+CPK
Sbjct: 564 GASINQYKTPRCVKFAPIVELLNSRVVSSYFSPKCPK 600

BLAST of Carg13552 vs. TrEMBL
Match: tr|A0A1R3KBU8|A0A1R3KBU8_9ROSI (GH3 auxin-responsive promoter OS=Corchorus olitorius OX=93759 GN=COLO4_09528 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 9.7e-260
Identity = 431/577 (74.70%), Postives = 499/577 (86.48%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++T++AD+VQ++VL EILSRNAHVEYLQ  GL+G  DR  FK ++PV++YE+++P
Sbjct: 24  LQFIEDVTSNADEVQKKVLEEILSRNAHVEYLQNHGLNGHTDRETFKKVMPVITYEDIQP 83

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           +IDRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 84  HIDRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLMPVMSQF 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKS+HFKDRPYD YT+YTSPN+TILC
Sbjct: 144 VPGLEKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSNHFKDRPYDPYTNYTSPNETILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAI+FLE HW LL NDIRTGT++ +I+
Sbjct: 204 PDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIRFLEKHWRLLCNDIRTGTLDAQIT 263

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++VM ILKPDP LAD IE EC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 264 DQSVREAVMTILKPDPKLADFIEAECGKDSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 323

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP----------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTL+PTMAYFEFLP           
Sbjct: 324 DYYSNGLPLVCTMYASSECYFGVNLKPLCKPSEVSYTLVPTMAYFEFLPVHRNNGVTNSI 383

Query: 370 -----LDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                L+ K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+LRV+GFKNKAPQFNF+
Sbjct: 384 SVPKSLNEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNKAPQFNFI 443

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQ+AV  A  +L+P++ +L EYTSYADT ++PGHYV++WE+S
Sbjct: 444 CRKNVVLSIDSDKTDEVELQSAVKNAVNHLLPFDATLAEYTSYADTTSIPGHYVLFWELS 503

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
              T  IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 504 LDGTTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 563

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS  VS+YFSP+CPK
Sbjct: 564 GASINQYKTPRCVKFAPIVELLNSRAVSSYFSPKCPK 600

BLAST of Carg13552 vs. TrEMBL
Match: tr|A0A1U8NWP7|A0A1U8NWP7_GOSHI (indole-3-acetic acid-amido synthetase GH3.6-like OS=Gossypium hirsutum OX=3635 GN=LOC107951777 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 9.7e-260
Identity = 433/578 (74.91%), Postives = 496/578 (85.81%), Query Frame = 0

Query: 9   LLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLK 68
           +LQ IE++T++AD+VQ++VL EILSRNAHVEYLQ  GL+G  DR  FK I+PV++YE+++
Sbjct: 24  ILQFIEDVTSNADEVQKKVLEEILSRNAHVEYLQTHGLNGHTDRETFKTIMPVITYEDIQ 83

Query: 69  PYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQ 128
           PY+DRIANGD SPIL S P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+Q
Sbjct: 84  PYVDRIANGDTSPILSSHPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLMPVMSQ 143

Query: 129 FIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTIL 188
           F+PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKS+HFKDRPYD YT+YTSPN+TIL
Sbjct: 144 FVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSTHFKDRPYDPYTNYTSPNETIL 203

Query: 189 CTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRI 248
           C DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  L NDIRTGTI+ +I
Sbjct: 204 CPDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWRFLCNDIRTGTIDSQI 263

Query: 249 SDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPT 308
           +D  VR++VM ILKPDP LAD IE ECSK +W GII RLWPNTKY+DVIVTGTMSQYIPT
Sbjct: 264 TDQSVREAVMKILKPDPQLADFIEAECSKDSWQGIITRLWPNTKYVDVIVTGTMSQYIPT 323

Query: 309 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR------- 368
           LDYY+NGLPLVCTMYASSECYFGVNLNPLC P+EVSYTLIPTMAYFEFLP+ R       
Sbjct: 324 LDYYTNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVQRNNGVNSS 383

Query: 369 ---------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNF 428
                    K  +ELVDLVDV+LG+EYELV+TTYSGLYRYRVGD+LRV+GFKN APQFNF
Sbjct: 384 ISVPKTLNEKEQQELVDLVDVKLGQEYELVVTTYSGLYRYRVGDVLRVAGFKNNAPQFNF 443

Query: 429 VCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEI 488
           +CRKNV+L ID+DK DEVELQNAV  A  + +P++ +L EYTSYADT T+PGHYV+YWE+
Sbjct: 444 ICRKNVVLCIDSDKTDEVELQNAVKNAVNHFLPFDATLAEYTSYADTTTIPGHYVLYWEL 503

Query: 489 SQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAIN 548
           S   T  IP  VFE+CCLTIEESLNSVYRQGRV DKSIGPLEIKI+E GTFDKLMDYAI+
Sbjct: 504 SLNGTTPIPPSVFEDCCLTIEESLNSVYRQGRVCDKSIGPLEIKIVEPGTFDKLMDYAIS 563

Query: 549 HGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
            G+SINQYKTPRCVK  PI+ELLNS  VS++FSP+CPK
Sbjct: 564 LGASINQYKTPRCVKFAPIVELLNSRAVSSFFSPKCPK 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948165.10.0e+0099.65indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata][more]
XP_023533206.10.0e+0098.42indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo][more]
XP_022970970.10.0e+0097.89indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima][more]
XP_022140599.15.0e-28482.79indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia... [more]
XP_023901431.12.0e-26174.70indole-3-acetic acid-amido synthetase GH3.6-like [Quercus suber] >POE49631.1 ind... [more]
Match NameE-valueIdentityDescription
AT5G54510.11.2e-25874.18Auxin-responsive GH3 family protein[more]
AT4G27260.16.6e-25472.62Auxin-responsive GH3 family protein[more]
AT4G37390.17.1e-21663.81Auxin-responsive GH3 family protein[more]
AT2G14960.13.9e-21464.41Auxin-responsive GH3 family protein[more]
AT2G23170.18.7e-21464.22Auxin-responsive GH3 family protein[more]
Match NameE-valueIdentityDescription
sp|Q9LSQ4|GH36_ARATH2.1e-25774.18Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
sp|O81829|GH35_ARATH1.2e-25272.62Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
sp|Q8LQM5|GH31_ORYSJ4.1e-22165.14Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
sp|A3BLS0|GH38_ORYSI2.1e-21764.90Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indi... [more]
sp|Q0D4Z6|GH38_ORYSJ2.1e-21764.90Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
tr|A0A2P4H061|A0A2P4H061_QUESU1.4e-26174.70Indole-3-acetic acid-amido synthetase gh3.6 OS=Quercus suber OX=58331 GN=CFP56_0... [more]
tr|A0A1U7YQ53|A0A1U7YQ53_NELNU3.9e-26175.39indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC10... [more]
tr|B9T670|B9T670_RICCO5.7e-26074.87Indole-3-acetic acid-amido synthetase GH3.6, putative OS=Ricinus communis OX=398... [more]
tr|A0A1R3KBU8|A0A1R3KBU8_9ROSI9.7e-26074.70GH3 auxin-responsive promoter OS=Corchorus olitorius OX=93759 GN=COLO4_09528 PE=... [more]
tr|A0A1U8NWP7|A0A1U8NWP7_GOSHI9.7e-26074.91indole-3-acetic acid-amido synthetase GH3.6-like OS=Gossypium hirsutum OX=3635 G... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004993GH3
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13552-RACarg13552-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 13..548
e-value: 6.6E-204
score: 678.5
IPR004993GH3 familyPANTHERPTHR31901FAMILY NOT NAMEDcoord: 8..570
NoneNo IPR availablePANTHERPTHR31901:SF2INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.5-RELATEDcoord: 8..570

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg13552Carg03153Silver-seed gourdcarcarB029
The following block(s) are covering this gene:
GeneOrganismBlock
Carg13552Cucurbita pepo (Zucchini)carcpeB0041
Carg13552Cucurbita pepo (Zucchini)carcpeB0045
Carg13552Cucurbita pepo (Zucchini)carcpeB0046
Carg13552Cucurbita pepo (Zucchini)carcpeB0047
Carg13552Wild cucumber (PI 183967)carcpiB0030
Carg13552Wild cucumber (PI 183967)carcpiB0034
Carg13552Cucumber (Chinese Long) v2carcuB0032
Carg13552Cucumber (Chinese Long) v3carcucB0030
Carg13552Cucumber (Chinese Long) v3carcucB0034
Carg13552Bottle gourd (USVL1VR-Ls)carlsiB028
Carg13552Bottle gourd (USVL1VR-Ls)carlsiB030
Carg13552Melon (DHL92) v3.5.1carmeB0033
Carg13552Melon (DHL92) v3.5.1carmeB0034
Carg13552Melon (DHL92) v3.6.1carmedB0035
Carg13552Melon (DHL92) v3.6.1carmedB0034
Carg13552Watermelon (Charleston Gray)carwcgB0028
Carg13552Watermelon (Charleston Gray)carwcgB0030
Carg13552Watermelon (Charleston Gray)carwcgB0032
Carg13552Watermelon (97103) v1carwmB0029
Carg13552Watermelon (97103) v1carwmB0032
Carg13552Watermelon (97103) v1carwmB0033
Carg13552Watermelon (97103) v2carwmbB0025
Carg13552Watermelon (97103) v2carwmbB0027
Carg13552Wax gourdcarwgoB0031
Carg13552Wax gourdcarwgoB0036
Carg13552Cucurbita pepo (Zucchini)carcpeB0040
Carg13552Silver-seed gourdcarcarB034
Carg13552Silver-seed gourdcarcarB035
Carg13552Silver-seed gourdcarcarB036
Carg13552Silver-seed gourdcarcarB037
Carg13552Cucumber (Gy14) v2carcgybB0032
Carg13552Cucumber (Gy14) v1carcgyB0028
Carg13552Cucumber (Gy14) v1carcgyB0035
Carg13552Cucurbita maxima (Rimu)carcmaB0043
Carg13552Cucurbita maxima (Rimu)carcmaB0037
Carg13552Cucurbita maxima (Rimu)carcmaB0038
Carg13552Cucurbita moschata (Rifu)carcmoB0037
Carg13552Cucurbita moschata (Rifu)carcmoB0039
Carg13552Cucurbita moschata (Rifu)carcmoB0040
Carg13552Cucurbita moschata (Rifu)carcmoB0045
Carg13552Cucurbita moschata (Rifu)carcmoB0046