CmaCh20G006460 (gene) Cucurbita maxima (Rimu)

NameCmaCh20G006460
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionIndole-3-acetic acid-amido synthetase GH3.6
LocationCma_Chr20 : 2992843 .. 2994368 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACACGTTGATGAAATCTCATCGGATGAGAGAGATAAGCAAGCATTACAGCATATTGAAGATGTCACGACACTTGCCAGCCAAATCCAACGACAAATCCTAACTCAAATTCTGTCAACAAATGCTGATGTCGAATACTTGCAACAAGATGGCCTTCATGGCTCTACCAAGCTTTCAACCTTCAAGAACCTTATACCTCTCATCTCTTACGAACAATTAAGACCTTATATAGATCGTATAGCCAACGGCGATGATTCCCCAATCCTATGTGCAAACCCCATCACAGAGTTCTTTATAAGGTACACTTCTTATTCTCTTGATGCGTTTCAATTCCATGCCTAAAATCAATGGTTTCTTGTAGCTCTGGGACATCTGGTGGAGAGCTCAAGTTGATTCCAATGTATGAAGAAGAGTTTATGAGAAGGCTATCATTTTTTGACTATGTGATGCCAAGAATGAAGCAGCTGTTTCCAAATAGTTTGGTGGGCAAAGGCAAAGGAATGCACTTTCAGTTTGCAAAGGTTGAGTTAAAGACAAGATCTGGAATTGTTGCTCACACTGCAATTACTAGTTTGTGCAAGAGAGCAAACCTCAATAATACAAGTCCTAAAGACATCATTCTCTGCACAGACATCATTACAAGGCTATGGCCGAACGCCAAATACATTAATGCTATTGTGACAGGAAGCATGGCACAGTACCTTCCCAGGCTAAATTACTACAGTAATCACCTTCCTATTGTTTCTGACCATTATGGTGCTTCAGAATGCTTCCTTGGCTTAAACTTAGATCCATTGTGTAGCCCTGATCGAACCTCCTACACTTTAATTCCCACCATGGCATATTTCGAGTTCCTGCCGATCGACAGGACGAAATCCAGTAGAGAAGTAAGCCGAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACATGAATATGAGCTCGTCATTACTACTTATGCTGGCAAGTCTGTCTTCTTCTTTTCAATATTGTACTTGGGAGTTCTGATTTAGTTCATGAATAAATTTACTGTTGCAGGGCTCTACCGTTATTGCATGGTGGACATTCTTCGTGTGACAGGATTTAAGAACAAAGCTCCAAATTTTAGTTTTGTATGCAGGAAAGACGTGGTTTTGAGCCTTGATATTGAGAAAACAGATGAAGCAGAGCTTCATGATGTTGTACAAGAAGCAGCTCGTCTTTTGAGCCCATTTGGGGCGGCCATTGCCGACTACACTAGCCATGCGGATAGCTCCAAGATTCCTGGACACACTGTGTTGTACTGGGAAATTCACGTTAAGGGGGGAATTTCTATTCCATCCTCAATCTATGAGGATTGCTGCTTTGCCATTGAAGAGTCTCTTAATGGACCGTACCGCTATCTACGATCTCATGAGAAGTGTATCAATCCATTGGAGATAAAGATTGTAAAGGTTGGAACCTTTGAGAAACTCATGGCGCAGGCACTGAATCGAGGAGCTTCAATGAGCCAATACAAGACGCCTCGATGTGTAAATCATTGA

mRNA sequence

ATGGCACACGTTGATGAAATCTCATCGGATGAGAGAGATAAGCAAGCATTACAGCATATTGAAGATGTCACGACACTTGCCAGCCAAATCCAACGACAAATCCTAACTCAAATTCTGTCAACAAATGCTGATGTCGAATACTTGCAACAAGATGGCCTTCATGGCTCTACCAAGCTTTCAACCTTCAAGAACCTTATACCTCTCATCTCTTACGAACAATTAAGACCTTATATAGATCGTATAGCCAACGGCGATGATTCCCCAATCCTATGTGCAAACCCCATCACAGAGTTCTTTATAAGCTCTGGGACATCTGGTGGAGAGCTCAAGTTGATTCCAATGTATGAAGAAGAGTTTATGAGAAGGCTATCATTTTTTGACTATGTGATGCCAAGAATGAAGCAGCTGTTTCCAAATAGTTTGGTGGGCAAAGGCAAAGGAATGCACTTTCAGTTTGCAAAGGTTGAGTTAAAGACAAGATCTGGAATTGTTGCTCACACTGCAATTACTAGTTTGTGCAAGAGAGCAAACCTCAATAATACAAGTCCTAAAGACATCATTCTCTGCACAGACATCATTACAAGGCTATGGCCGAACGCCAAATACATTAATGCTATTGTGACAGGAAGCATGGCACAGTACCTTCCCAGGCTAAATTACTACAGTAATCACCTTCCTATTGTTTCTGACCATTATGGTGCTTCAGAATGCTTCCTTGGCTTAAACTTAGATCCATTGTGTAGCCCTGATCGAACCTCCTACACTTTAATTCCCACCATGGCATATTTCGAGTTCCTGCCGATCGACAGGACGAAATCCAGTAGAGAAGTAAGCCGAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACATGAATATGAGCTCGTCATTACTACTTATGCTGGGCTCTACCGTTATTGCATGGTGGACATTCTTCGTGTGACAGGATTTAAGAACAAAGCTCCAAATTTTAGTTTTGTATGCAGGAAAGACGTGGTTTTGAGCCTTGATATTGAGAAAACAGATGAAGCAGAGCTTCATGATGTTGTACAAGAAGCAGCTCGTCTTTTGAGCCCATTTGGGGCGGCCATTGCCGACTACACTAGCCATGCGGATAGCTCCAAGATTCCTGGACACACTGTGTTGTACTGGGAAATTCACGTTAAGGGGGGAATTTCTATTCCATCCTCAATCTATGAGGATTGCTGCTTTGCCATTGAAGAGTCTCTTAATGGACCGTACCGCTATCTACGATCTCATGAGAAGTGTATCAATCCATTGGAGATAAAGATTGTAAAGGTTGGAACCTTTGAGAAACTCATGGCGCAGGCACTGAATCGAGGAGCTTCAATGAGCCAATACAAGACGCCTCGATGTGTAAATCATTGA

Coding sequence (CDS)

ATGGCACACGTTGATGAAATCTCATCGGATGAGAGAGATAAGCAAGCATTACAGCATATTGAAGATGTCACGACACTTGCCAGCCAAATCCAACGACAAATCCTAACTCAAATTCTGTCAACAAATGCTGATGTCGAATACTTGCAACAAGATGGCCTTCATGGCTCTACCAAGCTTTCAACCTTCAAGAACCTTATACCTCTCATCTCTTACGAACAATTAAGACCTTATATAGATCGTATAGCCAACGGCGATGATTCCCCAATCCTATGTGCAAACCCCATCACAGAGTTCTTTATAAGCTCTGGGACATCTGGTGGAGAGCTCAAGTTGATTCCAATGTATGAAGAAGAGTTTATGAGAAGGCTATCATTTTTTGACTATGTGATGCCAAGAATGAAGCAGCTGTTTCCAAATAGTTTGGTGGGCAAAGGCAAAGGAATGCACTTTCAGTTTGCAAAGGTTGAGTTAAAGACAAGATCTGGAATTGTTGCTCACACTGCAATTACTAGTTTGTGCAAGAGAGCAAACCTCAATAATACAAGTCCTAAAGACATCATTCTCTGCACAGACATCATTACAAGGCTATGGCCGAACGCCAAATACATTAATGCTATTGTGACAGGAAGCATGGCACAGTACCTTCCCAGGCTAAATTACTACAGTAATCACCTTCCTATTGTTTCTGACCATTATGGTGCTTCAGAATGCTTCCTTGGCTTAAACTTAGATCCATTGTGTAGCCCTGATCGAACCTCCTACACTTTAATTCCCACCATGGCATATTTCGAGTTCCTGCCGATCGACAGGACGAAATCCAGTAGAGAAGTAAGCCGAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACATGAATATGAGCTCGTCATTACTACTTATGCTGGGCTCTACCGTTATTGCATGGTGGACATTCTTCGTGTGACAGGATTTAAGAACAAAGCTCCAAATTTTAGTTTTGTATGCAGGAAAGACGTGGTTTTGAGCCTTGATATTGAGAAAACAGATGAAGCAGAGCTTCATGATGTTGTACAAGAAGCAGCTCGTCTTTTGAGCCCATTTGGGGCGGCCATTGCCGACTACACTAGCCATGCGGATAGCTCCAAGATTCCTGGACACACTGTGTTGTACTGGGAAATTCACGTTAAGGGGGGAATTTCTATTCCATCCTCAATCTATGAGGATTGCTGCTTTGCCATTGAAGAGTCTCTTAATGGACCGTACCGCTATCTACGATCTCATGAGAAGTGTATCAATCCATTGGAGATAAAGATTGTAAAGGTTGGAACCTTTGAGAAACTCATGGCGCAGGCACTGAATCGAGGAGCTTCAATGAGCCAATACAAGACGCCTCGATGTGTAAATCATTGA

Protein sequence

MAHVDEISSDERDKQALQHIEDVTTLASQIQRQILTQILSTNADVEYLQQDGLHGSTKLSTFKNLIPLISYEQLRPYIDRIANGDDSPILCANPITEFFISSGTSGGELKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTAITSLCKRANLNNTSPKDIILCTDIITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSHADSSKIPGHTVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNH
BLAST of CmaCh20G006460 vs. Swiss-Prot
Match: GH35_ARATH (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.5e-96
Identity = 168/280 (60.00%), Postives = 211/280 (75.36%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITRLWPN KY++ IVTG+M+QY+P L+YYSN LP+V   Y +SEC+ G+NL PLC P  
Sbjct: 298 IITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSE 357

Query: 252 TSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKLGHEYELVITTY 311
            SYTLIP+MAYFEFLP+ R              + +  +ELVDLVDVKLG EYELV+TTY
Sbjct: 358 VSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTY 417

Query: 312 AGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPF 371
           AGL RY + D+LRVTGFKNKAP FSF+CRK+VVLS+D +KTDE EL + V+ A   L PF
Sbjct: 418 AGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPF 477

Query: 372 GAAIADYTSHADSSKIPGHTVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSH 431
            A++++YTS+AD+S IPGH VL+WE+ + G   IP S++EDCC A+EES N  YR  R  
Sbjct: 478 DASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVS 537

Query: 432 EKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCV 461
           +K I PLEIKIV+ GTF+KLM  A++ GAS++QYKTPRCV
Sbjct: 538 DKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCV 577

BLAST of CmaCh20G006460 vs. Swiss-Prot
Match: GH36_ARATH (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.2e-95
Identity = 166/280 (59.29%), Postives = 208/280 (74.29%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITRLWPN KY++ IVTG+M+QY+P L+YYSN LP+V   Y +SEC+ G+NL PLC P  
Sbjct: 298 IITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSE 357

Query: 252 TSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKLGHEYELVITTY 311
            SYTLIP MAYFEFLP+ R              + +  +ELVDLVDVKLG EYELV+TTY
Sbjct: 358 VSYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTY 417

Query: 312 AGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPF 371
           AGLYRY + D+L V GFKN AP FSF+CRK+VVLS+D +KTDE EL + V+ A   L PF
Sbjct: 418 AGLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPF 477

Query: 372 GAAIADYTSHADSSKIPGHTVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSH 431
            A++++YTS+AD+S IPGH VL+WE+ + G   IP S++EDCC  IEESLN  YR  R  
Sbjct: 478 DASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVS 537

Query: 432 EKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCV 461
           +K I PLEIK+V+ GTF+KLM  A++ GAS++QYKTPRCV
Sbjct: 538 DKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCV 577

BLAST of CmaCh20G006460 vs. Swiss-Prot
Match: GH31_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 8.4e-92
Identity = 155/270 (57.41%), Postives = 209/270 (77.41%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           II RLWP  KYI+ IVTG+M+QY+P L +Y   LP+    Y +SEC+ GLNL+P+C P  
Sbjct: 298 IIRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSD 357

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSREVS-RELVDLVDVKLGHEYELVITTYAGLYRYCMVD 311
            +YTLIPTM Y+EFLP++   ++ E S R+LVDLVDVKLGHEYELV+TTY+GLYRY + D
Sbjct: 358 VAYTLIPTMCYYEFLPVNCNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGD 417

Query: 312 ILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSH 371
           +LRV GFKNKAP FSFV R++V LS+D +KTDE ELH  V  A + L+PFGA++ +YTS+
Sbjct: 418 VLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSY 477

Query: 372 ADSSKIPGHTVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEIK 431
           AD++ IPGH VL+WE+   G  ++P+S++E+CC ++EE+LN  YR  R+ ++ I PLEI+
Sbjct: 478 ADAATIPGHYVLFWELRA-GSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIR 537

Query: 432 IVKVGTFEKLMAQALNRGASMSQYKTPRCV 461
           +V  GTF+KLM  A++RGAS++QYK PRCV
Sbjct: 538 VVAEGTFDKLMDYAISRGASINQYKAPRCV 566

BLAST of CmaCh20G006460 vs. Swiss-Prot
Match: GH34_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.1e-91
Identity = 159/275 (57.82%), Postives = 206/275 (74.91%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           II R+WP+ KYI+ IVTG+MAQY+P L +Y   LP+    Y +SEC+ GLNL+P+C P  
Sbjct: 303 IIRRVWPSTKYIDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSE 362

Query: 252 TSYTLIPTMAYFEFLPIDR----TKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYC 311
            +YTLIPTM YFEFLP++       +     R LVDLVDVKLGHEYELV+TTY+GLYRY 
Sbjct: 363 VAYTLIPTMCYFEFLPVNSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYR 422

Query: 312 MVDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADY 371
           + D+LRV GFKN AP F+FV RK+V LS+D +KTDEAELH  V EA + L+PFGA++ +Y
Sbjct: 423 VGDVLRVAGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEY 482

Query: 372 TSHAD-SSKIPGHTVLYWEIH-VKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCIN 431
           TS+AD ++ IPGH VL+WE+    GG  +P+S++EDCC A+EE LN  YR  R+ ++ I 
Sbjct: 483 TSYADTATTIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIG 542

Query: 432 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCV 461
           PLEI++V  GTF+KLM  AL+RGAS++QYK PRCV
Sbjct: 543 PLEIRVVADGTFDKLMDYALSRGASINQYKAPRCV 577

BLAST of CmaCh20G006460 vs. Swiss-Prot
Match: GH38_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica GN=GH3.8 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 7.1e-83
Identity = 146/271 (53.87%), Postives = 194/271 (71.59%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITR+WPN KY++ IVTG+MAQY+P L +YS  LP+    Y +SEC+ GLNL P+C P  
Sbjct: 303 IITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSE 362

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVDI 311
            SYT++P M YFEFLP+D T ++   + +LVDL  V++G EYELVITTYAGL RY + D+
Sbjct: 363 VSYTIMPNMGYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDV 422

Query: 312 LRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSHA 371
           LRVTGF N AP F FV RK+V+LS++ +KTDEAEL   V+ A+ LL P GA++ +YTS A
Sbjct: 423 LRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQA 482

Query: 372 DSSKIPGHTVLYWEIHVK--GGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEI 431
            + +IPGH V+YWE+  K  G   + +     CC  +EE+LN  YR  R  +  I PLEI
Sbjct: 483 CTKRIPGHYVIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEI 542

Query: 432 KIVKVGTFEKLMAQALNRGASMSQYKTPRCV 461
           ++V+ GTFE+LM  A++RGAS++QYK PRCV
Sbjct: 543 RVVRPGTFEELMDYAISRGASINQYKVPRCV 573

BLAST of CmaCh20G006460 vs. TrEMBL
Match: A0A161YAJ1_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023148 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.4e-117
Identity = 250/516 (48.45%), Postives = 313/516 (60.66%), Query Frame = 1

Query: 6   EISSDERDKQALQH-IEDVTTLASQIQRQILTQILSTNADVEYLQQDGLHGSTKLSTFKN 65
           E  S+E     L H IE +   A ++Q+++L +ILS NA VEYLQ+ GL G T   +FK 
Sbjct: 8   ESESNEYTIFRLNHSIEYLMKNADEVQKRVLYEILSRNAGVEYLQRHGLGGRTDTHSFKK 67

Query: 66  LIPLISYEQLRPYIDRIANGDDSPILCANPITEFFISSGTSGGELKLIPMYEEEFMRRLS 125
            +PLI+YE ++P I+RIANGD SPILC+ PI+EF  SSGTSGGE KLIP  EEE  R+ S
Sbjct: 68  CVPLITYEDIQPDINRIANGDKSPILCSQPISEFLTSSGTSGGERKLIPTIEEELDRKSS 127

Query: 126 FFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTAITSLCKRANLNN---- 185
               +MP M Q  P   +  GK M+F F   E +T  GI+A    TS  K   L N    
Sbjct: 128 LNRLLMPIMNQCIPG--IYTGKAMNFIFLVPESRTPGGILAQPLSTSYNKNTQLVNYTST 187

Query: 186 -------------------------------TSPKDIIL------CTD-----IITRLWP 245
                                            P  ++       C+      IITRLWP
Sbjct: 188 NEAIYCLDGYQRTLNPEITDQLVRESVLTRVLKPDPVLADFIEAECSKKSWKGIITRLWP 247

Query: 246 NAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIP 305
           N K I AIVTG+M+QY+P L YY+N LPIVS  Y +SEC   LNLDPLC P   SYT IP
Sbjct: 248 NTKCIQAIVTGTMSQYIPTLEYYANGLPIVSSLYASSECCFALNLDPLCKPSDISYTFIP 307

Query: 306 TMAYFEFLPIDRTKSSREVSR---------ELVDLVDVKLGHEYELVITTYAGLYRYCMV 365
           TMA+FEFLP+    +S   +R         ELVDLVDVKLG  YE V+TTYAGLYRY + 
Sbjct: 308 TMAFFEFLPVRGEANSSPEARLPINEKKQQELVDLVDVKLGEIYEPVVTTYAGLYRYRVG 367

Query: 366 DILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHD-VVQEAARLLSPFGAAIADYT 425
           D+LRV GFKNKAP+F+FVCRK+V LS+D++KT E+ELH  VV+     L P    + DYT
Sbjct: 368 DLLRVAGFKNKAPHFNFVCRKNVALSIDVDKTYESELHSAVVKAEINHLRPLDGNLIDYT 427

Query: 426 SHADSSKIPGHTVLYWE--IHVKGGIS----IPSSIYEDCCFAIEESLNGPYRYLRSHEK 459
           ++AD + IPGH V++WE  ++  G I+    IP  ++EDCC  IEESLN  YR  R  +K
Sbjct: 428 AYADINTIPGHYVIFWEYIMYDNGSITTSQVIPPCVFEDCCLTIEESLNSVYREGRVADK 487

BLAST of CmaCh20G006460 vs. TrEMBL
Match: A0A0A0KAF9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G125240 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 8.1e-110
Identity = 198/276 (71.74%), Postives = 227/276 (82.25%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITRLWPNAKYINAIVTGSM+QY+P LNYY+N+LPIVSDHYG+SECFLGLNLDPLC+ D 
Sbjct: 291 IITRLWPNAKYINAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNHDE 350

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSR-EVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVD 311
            SYTLIPTMAYFEFLPID       EV+++LVDLVDVKLG EYELVITT+AGLYRYCM D
Sbjct: 351 VSYTLIPTMAYFEFLPIDMINDPNGEVNQQLVDLVDVKLGREYELVITTFAGLYRYCMGD 410

Query: 312 ILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSH 371
           I+RVTGFKNKAP+F FV RK+VVL++  EKTDEA LH  V+E  R++  FGA I DYT++
Sbjct: 411 IVRVTGFKNKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTY 470

Query: 372 ADSSKIPGHTVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCIN 431
           AD S IPGH VLYWE+ +     +    IPSS++EDCC  IE SLN  YR  RSHEKCIN
Sbjct: 471 ADLSTIPGHYVLYWELTMDDLKEQNSNDIPSSVFEDCCLDIENSLNLLYRLARSHEKCIN 530

Query: 432 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVN 462
           PLEIKIVK GTFEKLM  AL+RGAS++QYKTPRC+N
Sbjct: 531 PLEIKIVKAGTFEKLMQLALDRGASITQYKTPRCLN 566

BLAST of CmaCh20G006460 vs. TrEMBL
Match: K4CFK1_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 8.9e-109
Identity = 221/472 (46.82%), Postives = 294/472 (62.29%), Query Frame = 1

Query: 10  DERDKQALQHIEDVTTLASQIQRQILTQILSTNADVEYLQQDGL---HGSTKLSTFKNLI 69
           DE +K+ LQ  ED+T+  + +Q+ +L +ILS N+ VEY Q+ GL     +     FK ++
Sbjct: 13  DENNKKLLQFFEDITSNPNDVQKTVLHEILSRNSGVEYFQRHGLIINGNNDDYENFKKIM 72

Query: 70  PLISYEQLRPYIDRIANGDDSPILCANPITEFFISSGTSGGELKLIPMYEEEFMRRLSFF 129
           P+++YE L+P I+RIANGD S ILC+ PI+EF  SSGTSGGE KL+P  EEE  RR   +
Sbjct: 73  PIVTYEDLKPDINRIANGDRSSILCSLPISEFLTSSGTSGGERKLMPTIEEELGRRSLLY 132

Query: 130 DYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTAITSLCKRANL-------- 189
             +MP M +  P+  + KGKGM+F F K E KT  G++A   +TS  K +          
Sbjct: 133 SLLMPVMNKFVPD--LQKGKGMYFLFIKSEAKTPGGLLARPVLTSYYKSSYFKKRPYDPY 192

Query: 190 -NNTSPKDIILCTDIITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASEC 249
            N TSP + ILC D    ++   K        S    +PRL   + ++ ++         
Sbjct: 193 TNYTSPNETILCLDSYQSMYSQIKE-------SWKGIIPRLWPNTKYVDVI--------- 252

Query: 250 FLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSR-----ELVDLVDVKLGHE 309
                   +      +YTLIPTM +FEFLP+ R     E S      +LVDLVDVKLGHE
Sbjct: 253 --------VTGTIEVAYTLIPTMGFFEFLPVARDDQGPENSNKYDRDDLVDLVDVKLGHE 312

Query: 310 YELVITTYAGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQE 369
           YELV+TTYAGLYRY + DIL+V GFKNKAP F FVCRK+V LS+D +KT E ELHD V +
Sbjct: 313 YELVVTTYAGLYRYRVGDILKVAGFKNKAPQFHFVCRKNVALSIDSDKTYEVELHDAVLK 372

Query: 370 AARLLSPFGAAIADYTSHADSSKIPGHTVLYWEIH--VKGGISIPSSIYEDCCFAIEESL 429
           A+  L P+G ++ +YTS+AD+S  PGH VLYWEI+        IP+S++EDCC  IEESL
Sbjct: 373 ASTHLVPYGTSLTEYTSYADTSTNPGHYVLYWEINNDESDETLIPTSVFEDCCLTIEESL 432

Query: 430 NGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNH 463
           N  YR  R  +K I PLEIK+V+ GTF+KLM  A++ GAS++QYK PRCVN+
Sbjct: 433 NSVYRQCRVSDKSIGPLEIKVVENGTFDKLMDYAISNGASINQYKAPRCVNY 458

BLAST of CmaCh20G006460 vs. TrEMBL
Match: A0A0K9S163_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_011710 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.5e-103
Identity = 216/490 (44.08%), Postives = 289/490 (58.98%), Query Frame = 1

Query: 16  ALQHIEDVTTLASQIQRQILTQILSTNADVEYLQQDGL-HGSTKLSTFKNLIPLISYEQL 75
           AL+ IEDVT+   ++Q Q+L +ILS NA+VEYL++ GL  G      FK+ +P++ YE+L
Sbjct: 13  ALKFIEDVTSRCDEVQCQVLAEILSQNAEVEYLKRYGLPKGCVDREKFKSKVPVVMYEEL 72

Query: 76  RPYIDRIANGDDSPILCANPITEFFISSGTSGGELKLIPMYEEEFMRRLSFFDYVMPRMK 135
           +  I RIANGD SPILCA P T+ F SSGTSGGE  LIP  ++E  RR   +  ++P + 
Sbjct: 73  KSDIQRIANGDFSPILCARPFTQLFSSSGTSGGEPNLIPATDQEIPRRHQQYKVLLPLLS 132

Query: 136 QLFPNSLVGK---GKGMHFQFAKVELKTRSGIVA-----HTAITSLCKRANLNNTSPK-- 195
           Q FP    GK   G     +  +V+    S +V          + LC   +    S K  
Sbjct: 133 QCFPGMESGKLLCGFYQRHEVVRVDSHFASTLVVVIHFLRDHYSELCDDMSSGTLSSKIS 192

Query: 196 DIIL----------------------------CTDIITRLWPNAKYINAIVTGSMAQYLP 255
           D+ L                               I+ ++WPN KY++ I+TGSMAQY+P
Sbjct: 193 DMALRAHMAEHVVKQPEPELAKLVYNACKGENWEGILKKIWPNIKYLDIIITGSMAQYIP 252

Query: 256 RLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSRE 315
            LN+YS  +PIVS  Y  SE   G+NL+PLC P   SYTLIP MAYFEFLP++ + S RE
Sbjct: 253 ILNFYSGGVPIVSLKYTCSEGAFGINLNPLCDPYEVSYTLIPNMAYFEFLPLNTSSSDRE 312

Query: 316 VSR-ELVDLVDVKLGHEYELVITTYAGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLS 375
            S  +LVDL DVK+GHEYELV+TTY GLYR  + D+L  TGF N  P F FV R +V+LS
Sbjct: 313 QSSDQLVDLADVKVGHEYELVVTTYDGLYRLRVEDVLSPTGFYNSTPQFKFVRRNNVLLS 372

Query: 376 LDIEKTDEAELHDVVQEAARLLSPFGA-AIADYTSHADSSKIPGHTVLYWEIHVKGGISI 435
           +  EKT E+EL + +++   +L  FG   + DYTS+AD   IPGH V+Y E+      + 
Sbjct: 373 VHAEKTTESELQNTMEKVYDVLQNFGTIRVVDYTSYADVKTIPGHYVIYCELSTTTSDAT 432

Query: 436 P----SSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 461
                  + E CC  IE+S++  YRY R H   I PLEI++VK GTF+K+   A++RGAS
Sbjct: 433 NKLGLDGVMEKCCLTIEDSMSYVYRYYRVHNPAIGPLEIRVVKKGTFDKMRDFAISRGAS 492

BLAST of CmaCh20G006460 vs. TrEMBL
Match: A0A0A0LHA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G081190 PE=4 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 4.3e-103
Identity = 186/277 (67.15%), Postives = 220/277 (79.42%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           I+ +LWPN KYI AI TGS++QY+P LNYY+N+LPI SDHYG++ECFLGLNLDP+C P+ 
Sbjct: 293 IVPKLWPNTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNE 352

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVDI 311
           TSYTLIPTMAYFEFLPID T  + EV++ELVDLVDVKLG EYELVITT+AGLYR  + DI
Sbjct: 353 TSYTLIPTMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDI 412

Query: 312 LRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSHA 371
           +RVTGF NKAP FSFV RK+VVL+L+ EKT+E +L   V+ A  +L PFGA I DYTS+A
Sbjct: 413 VRVTGFTNKAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYA 472

Query: 372 DSSKIPGHTVLYWEIHVKGGIS-------IPSSIYEDCCFAIEESLNGPYRYLRSHEKCI 431
           D+S IPGH VLYWE+ + G          IPSS++ DCCFAIEES    YR  RSHEK I
Sbjct: 473 DTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTI 532

Query: 432 NPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVN 462
           NPLEI+IVK GTFEKLM  A+N GASM+QYKTPR +N
Sbjct: 533 NPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLN 569

BLAST of CmaCh20G006460 vs. TAIR10
Match: AT4G27260.1 (AT4G27260.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 354.0 bits (907), Expect = 1.4e-97
Identity = 168/280 (60.00%), Postives = 211/280 (75.36%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITRLWPN KY++ IVTG+M+QY+P L+YYSN LP+V   Y +SEC+ G+NL PLC P  
Sbjct: 298 IITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSE 357

Query: 252 TSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKLGHEYELVITTY 311
            SYTLIP+MAYFEFLP+ R              + +  +ELVDLVDVKLG EYELV+TTY
Sbjct: 358 VSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTY 417

Query: 312 AGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPF 371
           AGL RY + D+LRVTGFKNKAP FSF+CRK+VVLS+D +KTDE EL + V+ A   L PF
Sbjct: 418 AGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPF 477

Query: 372 GAAIADYTSHADSSKIPGHTVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSH 431
            A++++YTS+AD+S IPGH VL+WE+ + G   IP S++EDCC A+EES N  YR  R  
Sbjct: 478 DASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVS 537

Query: 432 EKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCV 461
           +K I PLEIKIV+ GTF+KLM  A++ GAS++QYKTPRCV
Sbjct: 538 DKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCV 577

BLAST of CmaCh20G006460 vs. TAIR10
Match: AT5G54510.1 (AT5G54510.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 351.7 bits (901), Expect = 7.0e-97
Identity = 166/280 (59.29%), Postives = 208/280 (74.29%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITRLWPN KY++ IVTG+M+QY+P L+YYSN LP+V   Y +SEC+ G+NL PLC P  
Sbjct: 298 IITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSE 357

Query: 252 TSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKLGHEYELVITTY 311
            SYTLIP MAYFEFLP+ R              + +  +ELVDLVDVKLG EYELV+TTY
Sbjct: 358 VSYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTY 417

Query: 312 AGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPF 371
           AGLYRY + D+L V GFKN AP FSF+CRK+VVLS+D +KTDE EL + V+ A   L PF
Sbjct: 418 AGLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPF 477

Query: 372 GAAIADYTSHADSSKIPGHTVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSH 431
            A++++YTS+AD+S IPGH VL+WE+ + G   IP S++EDCC  IEESLN  YR  R  
Sbjct: 478 DASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVS 537

Query: 432 EKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCV 461
           +K I PLEIK+V+ GTF+KLM  A++ GAS++QYKTPRCV
Sbjct: 538 DKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCV 577

BLAST of CmaCh20G006460 vs. TAIR10
Match: AT2G14960.1 (AT2G14960.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 308.1 bits (788), Expect = 8.9e-84
Identity = 150/272 (55.15%), Postives = 195/272 (71.69%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITR+WPN KY++ IVTG+MAQY+P L YYS  LP+    Y +SEC+ GLNL+P+  P  
Sbjct: 296 IITRIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSE 355

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVDI 311
            SYT++P MAYFEF+P+  TK+        V+LVDV +G EYELV+TTYAGL RY + DI
Sbjct: 356 VSYTIMPNMAYFEFIPLGGTKA--------VELVDVNIGKEYELVVTTYAGLCRYRVGDI 415

Query: 312 LRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLL-SPFGAAIADYTSH 371
           LRVTGF N AP F FV RK+V+LS+D +KTDE+EL   V+ A+ +L    G+ +A+YTS+
Sbjct: 416 LRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSY 475

Query: 372 ADSSKIPGHTVLYWEIHVKGGISIPS-SIYEDCCFAIEESLNGPYRYLRSHEKCINPLEI 431
           AD+S IPGH VLYWE+ V+ G   PS      CC  +EESLN  YR  R  +  + PLEI
Sbjct: 476 ADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEI 535

Query: 432 KIVKVGTFEKLMAQALNRGASMSQYKTPRCVN 462
           ++V+ GTFE+LM  A++RGAS++QYK PRCVN
Sbjct: 536 RVVRNGTFEELMDYAISRGASINQYKVPRCVN 559

BLAST of CmaCh20G006460 vs. TAIR10
Match: AT2G23170.1 (AT2G23170.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 303.1 bits (775), Expect = 2.9e-82
Identity = 146/271 (53.87%), Postives = 193/271 (71.22%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IIT++WPN KY++ IVTG+MAQY+P L YYS  LP+    Y +SE + G+NL P+C P  
Sbjct: 298 IITKIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSE 357

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVDI 311
            SYT++P MAYFEFLP     + +    ELV+L DV++G EYELVITTYAGL RY + DI
Sbjct: 358 VSYTIMPNMAYFEFLPHHEVPTEKS---ELVELADVEVGKEYELVITTYAGLNRYRVGDI 417

Query: 312 LRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSHA 371
           L+VTGF N AP F FV RK+V+LS++ +KTDEAEL   V+ A+ LL   G  + +YTS+A
Sbjct: 418 LQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYA 477

Query: 372 DSSKIPGHTVLYWEIHVKGGISIPS-SIYEDCCFAIEESLNGPYRYLRSHEKCINPLEIK 431
           ++  IPGH V+YWE+ VK   + P+  +   CC  +EESLN  YR  R  +K I PLEI+
Sbjct: 478 ETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIR 537

Query: 432 IVKVGTFEKLMAQALNRGASMSQYKTPRCVN 462
           +VK GTFE+LM  A++RGAS++QYK PRCV+
Sbjct: 538 VVKNGTFEELMDYAISRGASINQYKVPRCVS 565

BLAST of CmaCh20G006460 vs. TAIR10
Match: AT5G13360.3 (AT5G13360.3 Auxin-responsive GH3 family protein)

HSP 1 Score: 302.8 bits (774), Expect = 3.7e-82
Identity = 151/277 (54.51%), Postives = 194/277 (70.04%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           I+ RLWP AK I AIVTG+MAQY P L +YS  LP++S  YG+SECF GLNL+PL  P+ 
Sbjct: 364 IVKRLWPKAKCIEAIVTGTMAQYNPLLEFYSGGLPVISTFYGSSECFFGLNLNPLSKPNE 423

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSREVSRE---LVDLVDVKLGHEYELVITTYAGLYRYCM 311
            SYT+IP MAYFEFL +++   S     E   +VDLVDVK+GH+YE V+TT+AGLYRY +
Sbjct: 424 VSYTIIPCMAYFEFLEVEKDYESGHDPAENPVVVDLVDVKIGHDYEPVVTTFAGLYRYRL 483

Query: 312 VDILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYT 371
            D+LRVTGF N AP F FV R+ VVLS+D++KT + +L   V  A+ LL P    + D+T
Sbjct: 484 GDVLRVTGFYNNAPQFHFVGRQKVVLSIDMDKTYDEDLLKAVTNASLLLEPHDLMLMDFT 543

Query: 372 SHADSSKIPGHTVLYWEIHVK---GGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCIN 431
           S  DSS  PGH VLYWE+  K     + +  ++ E+CCF IEESL+  YR  R ++K I 
Sbjct: 544 SRVDSSSYPGHYVLYWELGRKVKDAKLELDQNVLEECCFTIEESLDAVYRKGRKNDKNIG 603

Query: 432 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNH 463
           PLEIK+VK G F+KLM   L+RG+S+SQYKTPR V +
Sbjct: 604 PLEIKVVKPGAFDKLMNFFLSRGSSVSQYKTPRSVTN 640

BLAST of CmaCh20G006460 vs. NCBI nr
Match: gi|296081237|emb|CBI17981.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 473.0 bits (1216), Expect = 5.9e-130
Identity = 239/453 (52.76%), Postives = 315/453 (69.54%), Query Frame = 1

Query: 10  DERDKQALQHIEDVTTLASQIQRQILTQILSTNADVEYLQQDGLHGSTKLSTFKNLIPLI 69
           D+++ +AL+ IEDVT  A ++Q Q+L++I++ N++VEYLQ+ GL+G     TFK ++P++
Sbjct: 22  DDKNIRALEFIEDVTMNAKEVQMQVLSEIITRNSNVEYLQRHGLNGRVDADTFKKIVPVV 81

Query: 70  SYEQLRPYIDRIANGDDSPILCANPITEFFISSGTSGGELKLIPMYEEEFMRRLSFFDYV 129
           +YE + P I+RIANGD SPILC+ PI+EF  SSGTSGGE KL+P  EEE  RR   +  +
Sbjct: 82  TYEDMLPDIERIANGDMSPILCSKPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLL 141

Query: 130 MPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTAITSLCKRANLNNTSPKDIILC 189
           MP M Q F   +  +   ++     V+ +   G+V      S    A+  +T        
Sbjct: 142 MPVMNQPFCVRIPTRACILNCFVGCVKTRKFFGLVQCLPPASSGPSASWKSTG------- 201

Query: 190 TDIITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP 249
                 LWPN KYI+ IVTG+M+QY+P L+YYSN LP+V   Y +SEC+ GLNL+PLC P
Sbjct: 202 ------LWPNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCKP 261

Query: 250 DRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYCMV 309
              SYTLIPTMAYFEFLP++R       + ELVDLVDVKLG EYELV+TTYAGLYRY + 
Sbjct: 262 SEVSYTLIPTMAYFEFLPVNRKNG---FTNELVDLVDVKLGQEYELVVTTYAGLYRYRVG 321

Query: 310 DILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTS 369
           DIL V GFKNKAP F F+CRK+V LS+D +KTDE EL + V+ AA  L  F A++ +YTS
Sbjct: 322 DILCVAGFKNKAPQFKFICRKNVALSIDSDKTDEFELQNAVKNAADHLLQFDASVTEYTS 381

Query: 370 HADSSKIPGHTVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEI 429
           +AD+S IPGH VLYWEI + G   IP S++EDCC  +EESLN  YR  R+ +K I PLEI
Sbjct: 382 YADTSTIPGHYVLYWEIGLSGATPIPPSVFEDCCLIMEESLNSVYRQGRASDKSIGPLEI 441

Query: 430 KIVKVGTFEKLMAQALNRGASMSQYKTPRCVNH 463
           +IV+ GTF+KLM  AL++GAS++QYKTPRCV +
Sbjct: 442 RIVEGGTFDKLMDYALSQGASINQYKTPRCVKY 458

BLAST of CmaCh20G006460 vs. NCBI nr
Match: gi|1021031705|gb|KZM89489.1| (hypothetical protein DCAR_023148 [Daucus carota subsp. sativus])

HSP 1 Score: 431.4 bits (1108), Expect = 2.0e-117
Identity = 250/516 (48.45%), Postives = 313/516 (60.66%), Query Frame = 1

Query: 6   EISSDERDKQALQH-IEDVTTLASQIQRQILTQILSTNADVEYLQQDGLHGSTKLSTFKN 65
           E  S+E     L H IE +   A ++Q+++L +ILS NA VEYLQ+ GL G T   +FK 
Sbjct: 8   ESESNEYTIFRLNHSIEYLMKNADEVQKRVLYEILSRNAGVEYLQRHGLGGRTDTHSFKK 67

Query: 66  LIPLISYEQLRPYIDRIANGDDSPILCANPITEFFISSGTSGGELKLIPMYEEEFMRRLS 125
            +PLI+YE ++P I+RIANGD SPILC+ PI+EF  SSGTSGGE KLIP  EEE  R+ S
Sbjct: 68  CVPLITYEDIQPDINRIANGDKSPILCSQPISEFLTSSGTSGGERKLIPTIEEELDRKSS 127

Query: 126 FFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTAITSLCKRANLNN---- 185
               +MP M Q  P   +  GK M+F F   E +T  GI+A    TS  K   L N    
Sbjct: 128 LNRLLMPIMNQCIPG--IYTGKAMNFIFLVPESRTPGGILAQPLSTSYNKNTQLVNYTST 187

Query: 186 -------------------------------TSPKDIIL------CTD-----IITRLWP 245
                                            P  ++       C+      IITRLWP
Sbjct: 188 NEAIYCLDGYQRTLNPEITDQLVRESVLTRVLKPDPVLADFIEAECSKKSWKGIITRLWP 247

Query: 246 NAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIP 305
           N K I AIVTG+M+QY+P L YY+N LPIVS  Y +SEC   LNLDPLC P   SYT IP
Sbjct: 248 NTKCIQAIVTGTMSQYIPTLEYYANGLPIVSSLYASSECCFALNLDPLCKPSDISYTFIP 307

Query: 306 TMAYFEFLPIDRTKSSREVSR---------ELVDLVDVKLGHEYELVITTYAGLYRYCMV 365
           TMA+FEFLP+    +S   +R         ELVDLVDVKLG  YE V+TTYAGLYRY + 
Sbjct: 308 TMAFFEFLPVRGEANSSPEARLPINEKKQQELVDLVDVKLGEIYEPVVTTYAGLYRYRVG 367

Query: 366 DILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHD-VVQEAARLLSPFGAAIADYT 425
           D+LRV GFKNKAP+F+FVCRK+V LS+D++KT E+ELH  VV+     L P    + DYT
Sbjct: 368 DLLRVAGFKNKAPHFNFVCRKNVALSIDVDKTYESELHSAVVKAEINHLRPLDGNLIDYT 427

Query: 426 SHADSSKIPGHTVLYWE--IHVKGGIS----IPSSIYEDCCFAIEESLNGPYRYLRSHEK 459
           ++AD + IPGH V++WE  ++  G I+    IP  ++EDCC  IEESLN  YR  R  +K
Sbjct: 428 AYADINTIPGHYVIFWEYIMYDNGSITTSQVIPPCVFEDCCLTIEESLNSVYREGRVADK 487

BLAST of CmaCh20G006460 vs. NCBI nr
Match: gi|659094768|ref|XP_008448232.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo])

HSP 1 Score: 418.7 bits (1075), Expect = 1.3e-113
Identity = 201/275 (73.09%), Postives = 229/275 (83.27%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITRLWPNAKYINA+VTGSM+QY+P LNYY+N+LPIVSDHYG+SECFLGLNLDPLC+PD 
Sbjct: 291 IITRLWPNAKYINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDE 350

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVDI 311
            SYTLIPTMAYFEFLPID      EV++ LVDLVDVKLGHEYELVITT+AGLYRYCM D+
Sbjct: 351 VSYTLIPTMAYFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDV 410

Query: 312 LRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSHA 371
           +RVTGFKNKAPNFSFV RK+VVL++  EKTDEA LH  V+E  R++  FGA I DYT++A
Sbjct: 411 VRVTGFKNKAPNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYA 470

Query: 372 DSSKIPGHTVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCINP 431
           D S IPGH VLYWE+ +     +    IP S++EDCC AIE SLN  YR  RSHEKCINP
Sbjct: 471 DISTIPGHYVLYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINP 530

Query: 432 LEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVN 462
           LEIKIVK GTFEKLM  ALNRGAS++QYKTPRC+N
Sbjct: 531 LEIKIVKAGTFEKLMQLALNRGASITQYKTPRCLN 565

BLAST of CmaCh20G006460 vs. NCBI nr
Match: gi|449444564|ref|XP_004140044.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis sativus])

HSP 1 Score: 405.6 bits (1041), Expect = 1.2e-109
Identity = 198/276 (71.74%), Postives = 227/276 (82.25%), Query Frame = 1

Query: 192 IITRLWPNAKYINAIVTGSMAQYLPRLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDR 251
           IITRLWPNAKYINAIVTGSM+QY+P LNYY+N+LPIVSDHYG+SECFLGLNLDPLC+ D 
Sbjct: 291 IITRLWPNAKYINAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNHDE 350

Query: 252 TSYTLIPTMAYFEFLPIDRTKSSR-EVSRELVDLVDVKLGHEYELVITTYAGLYRYCMVD 311
            SYTLIPTMAYFEFLPID       EV+++LVDLVDVKLG EYELVITT+AGLYRYCM D
Sbjct: 351 VSYTLIPTMAYFEFLPIDMINDPNGEVNQQLVDLVDVKLGREYELVITTFAGLYRYCMGD 410

Query: 312 ILRVTGFKNKAPNFSFVCRKDVVLSLDIEKTDEAELHDVVQEAARLLSPFGAAIADYTSH 371
           I+RVTGFKNKAP+F FV RK+VVL++  EKTDEA LH  V+E  R++  FGA I DYT++
Sbjct: 411 IVRVTGFKNKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTY 470

Query: 372 ADSSKIPGHTVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCIN 431
           AD S IPGH VLYWE+ +     +    IPSS++EDCC  IE SLN  YR  RSHEKCIN
Sbjct: 471 ADLSTIPGHYVLYWELTMDDLKEQNSNDIPSSVFEDCCLDIENSLNLLYRLARSHEKCIN 530

Query: 432 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVN 462
           PLEIKIVK GTFEKLM  AL+RGAS++QYKTPRC+N
Sbjct: 531 PLEIKIVKAGTFEKLMQLALDRGASITQYKTPRCLN 566

BLAST of CmaCh20G006460 vs. NCBI nr
Match: gi|902237607|gb|KNA24857.1| (hypothetical protein SOVF_011710 [Spinacia oleracea])

HSP 1 Score: 384.8 bits (987), Expect = 2.1e-103
Identity = 216/490 (44.08%), Postives = 289/490 (58.98%), Query Frame = 1

Query: 16  ALQHIEDVTTLASQIQRQILTQILSTNADVEYLQQDGL-HGSTKLSTFKNLIPLISYEQL 75
           AL+ IEDVT+   ++Q Q+L +ILS NA+VEYL++ GL  G      FK+ +P++ YE+L
Sbjct: 13  ALKFIEDVTSRCDEVQCQVLAEILSQNAEVEYLKRYGLPKGCVDREKFKSKVPVVMYEEL 72

Query: 76  RPYIDRIANGDDSPILCANPITEFFISSGTSGGELKLIPMYEEEFMRRLSFFDYVMPRMK 135
           +  I RIANGD SPILCA P T+ F SSGTSGGE  LIP  ++E  RR   +  ++P + 
Sbjct: 73  KSDIQRIANGDFSPILCARPFTQLFSSSGTSGGEPNLIPATDQEIPRRHQQYKVLLPLLS 132

Query: 136 QLFPNSLVGK---GKGMHFQFAKVELKTRSGIVA-----HTAITSLCKRANLNNTSPK-- 195
           Q FP    GK   G     +  +V+    S +V          + LC   +    S K  
Sbjct: 133 QCFPGMESGKLLCGFYQRHEVVRVDSHFASTLVVVIHFLRDHYSELCDDMSSGTLSSKIS 192

Query: 196 DIIL----------------------------CTDIITRLWPNAKYINAIVTGSMAQYLP 255
           D+ L                               I+ ++WPN KY++ I+TGSMAQY+P
Sbjct: 193 DMALRAHMAEHVVKQPEPELAKLVYNACKGENWEGILKKIWPNIKYLDIIITGSMAQYIP 252

Query: 256 RLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSRE 315
            LN+YS  +PIVS  Y  SE   G+NL+PLC P   SYTLIP MAYFEFLP++ + S RE
Sbjct: 253 ILNFYSGGVPIVSLKYTCSEGAFGINLNPLCDPYEVSYTLIPNMAYFEFLPLNTSSSDRE 312

Query: 316 VSR-ELVDLVDVKLGHEYELVITTYAGLYRYCMVDILRVTGFKNKAPNFSFVCRKDVVLS 375
            S  +LVDL DVK+GHEYELV+TTY GLYR  + D+L  TGF N  P F FV R +V+LS
Sbjct: 313 QSSDQLVDLADVKVGHEYELVVTTYDGLYRLRVEDVLSPTGFYNSTPQFKFVRRNNVLLS 372

Query: 376 LDIEKTDEAELHDVVQEAARLLSPFGA-AIADYTSHADSSKIPGHTVLYWEIHVKGGISI 435
           +  EKT E+EL + +++   +L  FG   + DYTS+AD   IPGH V+Y E+      + 
Sbjct: 373 VHAEKTTESELQNTMEKVYDVLQNFGTIRVVDYTSYADVKTIPGHYVIYCELSTTTSDAT 432

Query: 436 P----SSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGAS 461
                  + E CC  IE+S++  YRY R H   I PLEI++VK GTF+K+   A++RGAS
Sbjct: 433 NKLGLDGVMEKCCLTIEDSMSYVYRYYRVHNPAIGPLEIRVVKKGTFDKMRDFAISRGAS 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GH35_ARATH2.5e-9660.00Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana GN=GH3.5 PE=... [more]
GH36_ARATH1.2e-9559.29Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana GN=GH3.6 PE=... [more]
GH31_ORYSJ8.4e-9257.41Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
GH34_ORYSJ7.1e-9157.82Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
GH38_ORYSJ7.1e-8353.87Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
A0A161YAJ1_DAUCA1.4e-11748.45Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023148 PE=4 SV=1[more]
A0A0A0KAF9_CUCSA8.1e-11071.74Uncharacterized protein OS=Cucumis sativus GN=Csa_6G125240 PE=4 SV=1[more]
K4CFK1_SOLLC8.9e-10946.82Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1[more]
A0A0K9S163_SPIOL1.5e-10344.08Uncharacterized protein OS=Spinacia oleracea GN=SOVF_011710 PE=4 SV=1[more]
A0A0A0LHA0_CUCSA4.3e-10367.15Uncharacterized protein OS=Cucumis sativus GN=Csa_2G081190 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G27260.11.4e-9760.00 Auxin-responsive GH3 family protein[more]
AT5G54510.17.0e-9759.29 Auxin-responsive GH3 family protein[more]
AT2G14960.18.9e-8455.15 Auxin-responsive GH3 family protein[more]
AT2G23170.12.9e-8253.87 Auxin-responsive GH3 family protein[more]
AT5G13360.33.7e-8254.51 Auxin-responsive GH3 family protein[more]
Match NameE-valueIdentityDescription
gi|296081237|emb|CBI17981.3|5.9e-13052.76unnamed protein product [Vitis vinifera][more]
gi|1021031705|gb|KZM89489.1|2.0e-11748.45hypothetical protein DCAR_023148 [Daucus carota subsp. sativus][more]
gi|659094768|ref|XP_008448232.1|1.3e-11373.09PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo][more]
gi|449444564|ref|XP_004140044.1|1.2e-10971.74PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis sativus][more]
gi|902237607|gb|KNA24857.1|2.1e-10344.08hypothetical protein SOVF_011710 [Spinacia oleracea][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004993GH3
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G006460.1CmaCh20G006460.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPANTHERPTHR31901FAMILY NOT NAMEDcoord: 4..460
score: 5.6E
IPR004993GH3 familyPFAMPF03321GH3coord: 191..461
score: 8.9E
NoneNo IPR availablePANTHERPTHR31901:SF2INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.5-RELATEDcoord: 4..460
score: 5.6E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh20G006460CmaCh02G001400Cucurbita maxima (Rimu)cmacmaB477