Carg13552-RA (mRNA) Silver-seed gourd

NameCarg13552-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionIndole-3-acetic acid-amido synthetase GH3.3, putative
LocationCucurbita_argyrosperma_scaffold_053 : 213510 .. 216145 (-)
Sequence length1716
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCGTATCGGAATTCTTAACCAGGTGAGTGTAAACAAATCTATTGTAGTTATTCTTGTTAGAGACTACGACTCTTCACAATCGTTTGATATTGTCTACTTTCAGTATAAGCTCTCATAATTTTGAATAAAGTACACCATAGAGCCTCCCCTAAGGCTTACGGAGCCCTCGAACAACCTCCCCTTAATCGAGGCTCAACTCTTTGTTTGGAGCTTTTGAACAAAGTAAACCCTTCGTTCAATACTTTAGTCACTTTTGACTACACTTTCGAGGCTCACAATTTCTTTGTTCGACATTTGAGAATTCTATTGATATGGCTAAGTTAAGGGCATGGCTCTAATACCAGGATAGGAACCATGACTCTCCACAATGGTATGATATTGTCCATTTTGAGCATAAGCTCTCGTGGCTTTGCTTTTGGTTTCTCCAAAAGACATCATACTTAGCCGATGATCAACCCCTCAATTAGCCGTTGTGGTACTCCTCTCCCAATTGTGTTTGAACTCAATTGTTGTTTGTGTTTTTCTATTCTAGTTCCGGAACATCGGGAGGGGAGAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGCGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTATCATACCTAGGCTGTGGCCAAATACAAAGTATATTGATGTTATTGTGACAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGGTATGTTTTTGTTGAGACATAGAGAATCTATGGGTCTGCTGCCGATTTATAGCTTATTCAATTGTTATAACTGTTAGACACAATTGTTTAGACCCTACGGAGCCTGTAGACTCAATTGTTTAGACTCTACGGAGTTGGTGTCTATGACAGTGTGTATAGTTTTCCTGTATATGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCATAGACAGTGTGTATAGTTTTCCTGTCTGGTTGATTAAGACTCCTACATGAATGAATAATATTTGGTTCAGGTATCTAAGATAACCTATCTTATTTGATATTGCAGGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACACCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATTATAG

mRNA sequence

ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCGTATCGGAATTCTTAACCAGTTCCGGAACATCGGGAGGGGAGAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGCGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTATCATACCTAGGCTGTGGCCAAATACAAAGTATATTGATGTTATTGTGACAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACACCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATTATAG

Coding sequence (CDS)

ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCGTATCGGAATTCTTAACCAGTTCCGGAACATCGGGAGGGGAGAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGCGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTATCATACCTAGGCTGTGGCCAAATACAAAGTATATTGATGTTATTGTGACAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACACCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATTATAG

Protein sequence

MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKL
BLAST of Carg13552-RA vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 568/570 (99.65%), Postives = 569/570 (99.82%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           TGTINPRI DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG
Sbjct: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420
           RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL
Sbjct: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420

Query: 421 SIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480
           SID+DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI
Sbjct: 421 SIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480

Query: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540
           PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY
Sbjct: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540

Query: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           KTPRCVKHEPILELLNSYVVSNYFSPRCPK
Sbjct: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 570

BLAST of Carg13552-RA vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 561/570 (98.42%), Postives = 568/570 (99.65%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MP +DEGA+LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 8   MPERDEGAILQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 67

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS
Sbjct: 68  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 127

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLN+GKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 128 LLMPVMNQFIPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 187

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMY QFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 188 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 247

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG
Sbjct: 248 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 307

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 308 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 367

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420
           R+MSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL
Sbjct: 368 REMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 427

Query: 421 SIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480
           SID+DKVDEVELQNAVMEASKNL+PYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI
Sbjct: 428 SIDSDKVDEVELQNAVMEASKNLVPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 487

Query: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540
           PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLE+KIIENGTFDKLMDYAINHGSSINQY
Sbjct: 488 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGSSINQY 547

Query: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           KTPRCVKHEPILELLNSYVVSNYFSPRCPK
Sbjct: 548 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 577

BLAST of Carg13552-RA vs. NCBI nr
Match: XP_022970970.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 558/570 (97.89%), Postives = 565/570 (99.12%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP+SEFLTSSGTSGGERKLMPTT+EELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMY QFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGII RLWPNTKY+DVIVTG
Sbjct: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLD YSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420
           RKMSEELVDLVDVELGKEYEL+ITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL
Sbjct: 361 RKMSEELVDLVDVELGKEYELIITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVIL 420

Query: 421 SIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480
           SID+DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI
Sbjct: 421 SIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAI 480

Query: 481 PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQY 540
           PD VFENCCLTIEESLNSVYRQGRVSDKSIGPLE+KIIENGTFDKLMDYAINHGSSINQY
Sbjct: 481 PDWVFENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGSSINQY 540

Query: 541 KTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           KTPRCVK EPILELLNSYVVSNYFSP+CPK
Sbjct: 541 KTPRCVKREPILELLNSYVVSNYFSPKCPK 570

BLAST of Carg13552-RA vs. NCBI nr
Match: XP_022140599.1 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia])

HSP 1 Score: 986.1 bits (2548), Expect = 5.0e-284
Identity = 481/581 (82.79%), Postives = 521/581 (89.67%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL + GLDG MD   FK  +PVVSY+++KP
Sbjct: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTMPVVSYDDIKP 86

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           Y+DRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPTTEEEL+R+S LYSLLMPVMNQF
Sbjct: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           IPGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           TDSYQSMY QFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI+
Sbjct: 207 TDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DPLVRQSV+NILKPDP LADLIE ECSKGTW GII RLW NTKYIDVIVTGTMSQYIPTL
Sbjct: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 369
           DYYSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386

Query: 370 -------RKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                  +K  +ELVDLVDV+LGKEYELVITTY+GLYRYRVGDILRVSGFKN APQFNFV
Sbjct: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTYAGLYRYRVGDILRVSGFKNNAPQFNFV 446

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNVILSID+DKVDEVELQNAVMEASKNLIPY   ++EYTSYADT T+PGHYVIYWEIS
Sbjct: 447 CRKNVILSIDSDKVDEVELQNAVMEASKNLIPYGACVVEYTSYADTVTIPGHYVIYWEIS 506

Query: 490 QKE-----TNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMD 549
            KE      N IP  VFE CCL++EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLMD
Sbjct: 507 LKEATANNNNVIPGSVFEKCCLSMEESLNSVYRQGRVSDKSIGPLEMKIVETGTFDKLMD 566

Query: 550 YAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
           YA+NHG+SINQYKTPRCVKH PILELLNSYVV NYFSP+CP
Sbjct: 567 YALNHGASINQYKTPRCVKHAPILELLNSYVVDNYFSPKCP 606

BLAST of Carg13552-RA vs. NCBI nr
Match: XP_023901431.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Quercus suber] >POE49631.1 indole-3-acetic acid-amido synthetase gh3.6 [Quercus suber])

HSP 1 Score: 911.0 bits (2353), Expect = 2.0e-261
Identity = 437/585 (74.70%), Postives = 507/585 (86.67%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           + GK++ A LQ IE++T++AD+VQRRVL  ILSR+AHVEYLQ+ GL+GC D+  FK +VP
Sbjct: 22  LDGKNKKA-LQFIEDVTSNADEVQRRVLAGILSRSAHVEYLQRHGLNGCTDQETFKKVVP 81

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VV+YE+LKP IDRIANGDASPILCS+P+SEFLTSSGTSGGERKLMPT EEEL+RRS LYS
Sbjct: 82  VVTYEDLKPEIDRIANGDASPILCSQPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 141

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQ +PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKSSHF DRPYD YT+Y
Sbjct: 142 LLMPVMNQCVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFMDRPYDPYTNY 201

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPN+TILC DSYQSMY Q LCGL  + QVLRVG+VFASGFIRA+KFL+ +W+LL NDIR
Sbjct: 202 TSPNETILCPDSYQSMYSQLLCGLYQNTQVLRVGAVFASGFIRALKFLQMNWLLLCNDIR 261

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           +GT++P+I+D  VR++VM IL+P+P LAD IE EC K +W+GII RLWPNTKYIDVIVTG
Sbjct: 262 SGTLDPKITDSSVREAVMKILEPNPRLADFIEDECGKDSWSGIITRLWPNTKYIDVIVTG 321

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC P+EVSYTLIPTM Y+EFLP++
Sbjct: 322 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCKPSEVSYTLIPTMCYYEFLPVN 381

Query: 361 RKM----------------SEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFK 420
           RK                 +++LVDLVDV+LG+EYELV+TTY+GLYRYRVGDILRV+GFK
Sbjct: 382 RKSGFNESFSKPVSLNDKENQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDILRVAGFK 441

Query: 421 NKAPQFNFVCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPG 480
           NKAPQFNFVCRKNV+LSID+DK DEVELQNAV  A+ NL+ ++ SL EYTSYADT TVPG
Sbjct: 442 NKAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNAANNLLSFDASLTEYTSYADTSTVPG 501

Query: 481 HYVIYWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFD 540
           HYV+YWEIS      IP  VFE+CCLT+EES NSVYRQGRVSDKSIGPLEIKI+E+GTFD
Sbjct: 502 HYVLYWEISLSRATPIPPSVFEDCCLTVEESFNSVYRQGRVSDKSIGPLEIKIVESGTFD 561

Query: 541 KLMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
            LMDYA+  G+SINQYK PRCVK+ PILELLNS VV+NYFSP+CP
Sbjct: 562 SLMDYAVGQGASINQYKAPRCVKYAPILELLNSKVVANYFSPKCP 605

BLAST of Carg13552-RA vs. TAIR10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 889.4 bits (2297), Expect = 1.2e-258
Identity = 428/577 (74.18%), Postives = 492/577 (85.27%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++TT+AD VQRRVL EILSRNA VEYL++ GL+G  DR  FK+I+PVV+YE+++P
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD S +LCS P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPNQTILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  LA DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++V  ILKPDP LAD +E EC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+L V+GFKN APQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIK++E+GTFDKLMDYAI+ 
Sbjct: 505 LNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552-RA vs. TAIR10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 873.6 bits (2256), Expect = 6.6e-254
Identity = 419/577 (72.62%), Postives = 485/577 (84.06%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IEE+T++ADQVQ       L+RNA VEYL++  L+G  DR  FKNI+PV++YE+++P
Sbjct: 25  LQLIEELTSNADQVQXXXXXXXLTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPIL S+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL  GKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  Q+VLRVG+VFASGFIRAIKFLE HW+ L  DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  ILKP P LAD +EFEC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP+MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GL RYRVGD+LRV+GFKNKAPQF+F+
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCL +EES N+VYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 505 LDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552-RA vs. TAIR10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 747.3 bits (1928), Expect = 7.1e-216
Identity = 365/572 (63.81%), Postives = 449/572 (78.50%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IEE+T + D VQ +VL EIL+RN++ EYL++  LDG +DR  FK+ VPVV+YE+LKP
Sbjct: 24  LKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYEDLKP 83

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I RI+NGD SPIL S P++EFLTSSGTS GERKLMPT EE+L RR  LYSLLMPVMN +
Sbjct: 84  EIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPVMNLY 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSES T GGLPARPVLTSYYKS HFK RPYD Y  YTSPN+ ILC
Sbjct: 144 VPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNEAILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DS QSMY Q LCGL +  +VLR+G+VFASG +RAI FL+ +W  LA DI TGT++ RI 
Sbjct: 204 SDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLSSRIF 263

Query: 250 DPLVRQSVMNIL-KPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPT 309
           DP ++  +  IL KPD  LA+ +   CS+  W GII ++WPNTKY+DVIVTG M+QYIPT
Sbjct: 264 DPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMAQYIPT 323

Query: 310 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP--------LD 369
           L+YYS GLP+ CTMYASSE YFG+NL P+C P+EVSYT++P MAYFEFLP         +
Sbjct: 324 LEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDGAAE 383

Query: 370 RKMSE-ELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVI 429
             + E  LV+L +VE+GKEYELVITTY+GLYRYRVGDILRV+GF N APQF F+ RKNV+
Sbjct: 384 ASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRKNVL 443

Query: 430 LSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWE-ISQKETN 489
           LS+++DK DE ELQ AV  AS+      T ++EYTSYA+T T+PGHYVIYWE + + ++N
Sbjct: 444 LSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELLGRDQSN 503

Query: 490 AI-PDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSI 549
           A+  + V   CCL +EESLNSVYRQ RV+DKSIGPLEI+++ NGTF++LMDYAI+ G+SI
Sbjct: 504 ALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAISRGASI 563

Query: 550 NQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
           NQYK PRCV   PI+ELL+S VVS +FSP  P
Sbjct: 564 NQYKVPRCVSFTPIMELLDSRVVSAHFSPSLP 595

BLAST of Carg13552-RA vs. TAIR10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 741.5 bits (1913), Expect = 3.9e-214
Identity = 362/562 (64.41%), Postives = 436/562 (77.58%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IEE+T +AD VQ  +L EIL+RNA  EYL++  L G  DR  FK  +PV++YE+L+P
Sbjct: 23  LRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVITYEDLQP 82

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I RIA+GD SPIL + P+SEFLTSSGTS GERKLMPT +EEL RR  LYSLLMPVMN +
Sbjct: 83  EIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLY 142

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLFVKSE+ TPGGLPARPVLTSYYKS HF+ RPYD Y  YTSPN+ ILC
Sbjct: 143 VPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSPNEAILC 202

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DS+QSMY Q LCGL     VLRVG+VFASG +RAI+FL+ HW   A+DI  G ++  I+
Sbjct: 203 PDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGCLDSEIT 262

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP +RQ +  ILKPDPVLA+ I  EC    W  II R+WPNTKY+DVIVTG M+QYIPTL
Sbjct: 263 DPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMAQYIPTL 322

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRKMSEELVD 369
           +YYS GLP+ CTMYASSECYFG+NLNP+  P+EVSYT++P MAYFEF+PL      + V+
Sbjct: 323 EYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG---GTKAVE 382

Query: 370 LVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDTDKVDE 429
           LVDV +GKEYELV+TTY+GL RYRVGDILRV+GF N APQF+FV RKNV+LSID+DK DE
Sbjct: 383 LVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDE 442

Query: 430 VELQNAVMEASKNL-IPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPD-LVFEN 489
            ELQ AV  AS  L     + + EYTSYADT T+PGHYV+YWE+  ++    P       
Sbjct: 443 SELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDGARQPSHETLTR 502

Query: 490 CCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVK 549
           CCL +EESLNSVYRQ RV+D S+GPLEI+++ NGTF++LMDYAI+ G+SINQYK PRCV 
Sbjct: 503 CCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVN 562

Query: 550 HEPILELLNSYVVSNYFSPRCP 570
             PI+ELL+S VVS +FSP  P
Sbjct: 563 FTPIVELLDSRVVSAHFSPSLP 581

BLAST of Carg13552-RA vs. TAIR10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 740.3 bits (1910), Expect = 8.7e-214
Identity = 368/573 (64.22%), Postives = 447/573 (78.01%), Query Frame = 0

Query: 2   PGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPV 61
           P   +   L+ IEE+T + D VQ++V+ EILSRN+  EYL++ GL G  DR  FK  VPV
Sbjct: 15  PSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPV 74

Query: 62  VSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSL 121
           V Y++LKP I RIANGD S IL S P++EFLTSSGTS GERKLMPT +E++ RR  LYSL
Sbjct: 75  VIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSL 134

Query: 122 LMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYT 181
           LMPVMN ++PGL+KGK +YFLFVK+ES TPGGLPARPVLTSYYKS  FK RP D Y  YT
Sbjct: 135 LMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYT 194

Query: 182 SPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRT 241
           SPN+ ILC DS QSMY Q LCGL +  +VLR+G+VFASG +RAI FL+T+W  LA+DI T
Sbjct: 195 SPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDIST 254

Query: 242 GTINPRISDPLVRQSVMNIL-KPDPVLADLIEFECSK-GTWAGIIPRLWPNTKYIDVIVT 301
           GT++ RISDP +++S+  IL KPD  LAD I   C +  +W GII ++WPNTKY+DVIVT
Sbjct: 255 GTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVT 314

Query: 302 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 361
           G M+QYIP L+YYS GLP+ CTMYASSE YFG+NL P+C P+EVSYT++P MAYFEFLP 
Sbjct: 315 GAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPH 374

Query: 362 DRKMSE--ELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKN 421
               +E  ELV+L DVE+GKEYELVITTY+GL RYRVGDIL+V+GF N APQF FV RKN
Sbjct: 375 HEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRKN 434

Query: 422 VILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK-E 481
           V+LSI++DK DE ELQ+AV  AS  L    T ++EYTSYA+T T+PGHYVIYWE+  K +
Sbjct: 435 VLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKDQ 494

Query: 482 TNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSS 541
           TN   D V   CCL +EESLNSVYRQ RV+DKSIGPLEI++++NGTF++LMDYAI+ G+S
Sbjct: 495 TNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGAS 554

Query: 542 INQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
           INQYK PRCV   PI+ELL+S VVS +FSP  P
Sbjct: 555 INQYKVPRCVSFTPIMELLDSRVVSTHFSPALP 587

BLAST of Carg13552-RA vs. Swiss-Prot
Match: sp|Q9LSQ4|GH36_ARATH (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 2.1e-257
Identity = 428/577 (74.18%), Postives = 492/577 (85.27%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++TT+AD VQRRVL EILSRNA VEYL++ GL+G  DR  FK+I+PVV+YE+++P
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD S +LCS P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPNQTILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  LA DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++V  ILKPDP LAD +E EC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+L V+GFKN APQF+F+
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIK++E+GTFDKLMDYAI+ 
Sbjct: 505 LNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552-RA vs. Swiss-Prot
Match: sp|O81829|GH35_ARATH (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 1.2e-252
Identity = 419/577 (72.62%), Postives = 485/577 (84.06%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IEE+T++ADQVQ       L+RNA VEYL++  L+G  DR  FKNI+PV++YE+++P
Sbjct: 25  LQLIEELTSNADQVQXXXXXXXLTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPIL S+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL  GKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
           +DSYQSMY Q LCGLC  Q+VLRVG+VFASGFIRAIKFLE HW+ L  DIRTGT++  I+
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  ILKP P LAD +EFEC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP+MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                   K  +ELVDLVDV+LG+EYELV+TTY+GL RYRVGD+LRV+GFKNKAPQF+F+
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAPQFSFI 444

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+ 
Sbjct: 445 CRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELC 504

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
                 IP  VFE+CCL +EES N+VYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 505 LDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 564

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VV +YFSP+CPK
Sbjct: 565 GASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

BLAST of Carg13552-RA vs. Swiss-Prot
Match: sp|Q8LQM5|GH31_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 4.1e-221
Identity = 370/568 (65.14%), Postives = 451/568 (79.40%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRM-AFKNIVPVVSYENLK 69
           L+ IE +T +A QVQRRVL EIL++NA  EYL++ G+ G  D + AF+ +VP+V+YE L+
Sbjct: 24  LEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEGLQ 83

Query: 70  PYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQ 129
           P I RIANGD SPI   +P+SEFLTSSGTSGGERKLMPT  +E+ RRS LYSLLMPVM+Q
Sbjct: 84  PDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQ 143

Query: 130 FIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTIL 189
            + GL+KGK MY LFVK+ES TPGGL ARPVLTSYY+S  F DRP D YT YTSP++ IL
Sbjct: 144 SVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAIL 203

Query: 190 CTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRI 249
           C DSYQSMY Q LCGL     VLRVG+VFASGF+RAI FLE HW  L +DIRTG ++P I
Sbjct: 204 CVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDPEI 263

Query: 250 SDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPT 309
           +D +VR +V  +L+ DP LAD IE EC++ +W GII RLWP TKYIDVIVTGTMSQYIPT
Sbjct: 264 TDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIPT 323

Query: 310 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD------RK 369
           L++Y  GLPL CTMYASSECYFG+NLNP+C P++V+YTLIPTM Y+EFLP++        
Sbjct: 324 LEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATAEA 383

Query: 370 MSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSI 429
              +LVDLVDV+LG EYELV+TTYSGLYRYRVGD+LRV+GFKNKAP F+FV R+NV LS+
Sbjct: 384 SHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVALSV 443

Query: 430 DTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPD 489
           D+DK DE EL  AV  A ++L P+  SL+EYTSYAD  T+PGHYV++WE+    T A+P 
Sbjct: 444 DSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAGST-AVPA 503

Query: 490 LVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKT 549
            VFE CCL++EE+LNSVYRQGR  D+SIGPLEI+++  GTFDKLMDYAI+ G+SINQYK 
Sbjct: 504 SVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQYKA 563

Query: 550 PRCVKHEPILELLNSYVVSNYFSPRCPK 571
           PRCV+  P++ELL++ V   YFSP+CPK
Sbjct: 564 PRCVRPGPVVELLDARVQGKYFSPKCPK 590

BLAST of Carg13552-RA vs. Swiss-Prot
Match: sp|A3BLS0|GH38_ORYSI (Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=2 SV=2)

HSP 1 Score: 756.5 bits (1952), Expect = 2.1e-217
Identity = 368/567 (64.90%), Postives = 446/567 (78.66%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GLDG  DR AF+  VPVVSY++L+P
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           YI RIANGD SPIL + PVSEFLTSSGTS GERKLMPT  +EL RR  LYSLLMPVMN +
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSE+ TPGGL ARPVLTSYYKS HFK+RPYD Y +YTSP   ILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            D++QSMY Q +CGLC    VLR+G+VFASG +RAI+FL+ +W  LA+DI +G + PR++
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  IL PDP LA LI  ECSKG WAGII R+WPNTKY+DVIVTG M+QYIPTL
Sbjct: 270 DPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTL 329

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRK-----MS 369
           ++YS GLP+ CTMYASSECYFG+NL P+C+P+EVSYT++P M YFEFLP+D        +
Sbjct: 330 EFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDA 389

Query: 370 EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDT 429
            +LVDL  VE+G+EYELVITTY+GL RYRVGD+LRV+GF N APQF FV RKNV+LSI++
Sbjct: 390 TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIES 449

Query: 430 DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNA--IPD 489
           DK DE ELQ AV  AS  L P+  S++EYTS A T  +PGHYVIYWE+  K   A  +  
Sbjct: 450 DKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDA 509

Query: 490 LVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKT 549
                CCL +EE+LN+VYRQ RV+D SIGPLEI+++  GTF++LMDYAI+ G+SINQYK 
Sbjct: 510 DTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKV 569

Query: 550 PRCVKHEPILELLNSYVVSNYFSPRCP 570
           PRCV   PI+ELL+S VVS++FSP  P
Sbjct: 570 PRCVTFPPIVELLDSRVVSSHFSPALP 596

BLAST of Carg13552-RA vs. Swiss-Prot
Match: sp|Q0D4Z6|GH38_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=2 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.1e-217
Identity = 368/567 (64.90%), Postives = 446/567 (78.66%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GLDG  DR AF+  VPVVSY++L+P
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           YI RIANGD SPIL + PVSEFLTSSGTS GERKLMPT  +EL RR  LYSLLMPVMN +
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSE+ TPGGL ARPVLTSYYKS HFK+RPYD Y +YTSP   ILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            D++QSMY Q +CGLC    VLR+G+VFASG +RAI+FL+ +W  LA+DI +G + PR++
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR++V  IL PDP LA LI  ECSKG WAGII R+WPNTKY+DVIVTG M+QYIPTL
Sbjct: 270 DPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTL 329

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRK-----MS 369
           ++YS GLP+ CTMYASSECYFG+NL P+C+P+EVSYT++P M YFEFLP+D        +
Sbjct: 330 EFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDA 389

Query: 370 EELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDT 429
            +LVDL  VE+G+EYELVITTY+GL RYRVGD+LRV+GF N APQF FV RKNV+LSI++
Sbjct: 390 TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIES 449

Query: 430 DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNA--IPD 489
           DK DE ELQ AV  AS  L P+  S++EYTS A T  +PGHYVIYWE+  K   A  +  
Sbjct: 450 DKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDA 509

Query: 490 LVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKT 549
                CCL +EE+LN+VYRQ RV+D SIGPLEI+++  GTF++LMDYAI+ G+SINQYK 
Sbjct: 510 DTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKV 569

Query: 550 PRCVKHEPILELLNSYVVSNYFSPRCP 570
           PRCV   PI+ELL+S VVS++FSP  P
Sbjct: 570 PRCVTFPPIVELLDSRVVSSHFSPALP 596

BLAST of Carg13552-RA vs. TrEMBL
Match: tr|A0A2P4H061|A0A2P4H061_QUESU (Indole-3-acetic acid-amido synthetase gh3.6 OS=Quercus suber OX=58331 GN=CFP56_05848 PE=4 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 1.4e-261
Identity = 437/585 (74.70%), Postives = 507/585 (86.67%), Query Frame = 0

Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           + GK++ A LQ IE++T++AD+VQRRVL  ILSR+AHVEYLQ+ GL+GC D+  FK +VP
Sbjct: 22  LDGKNKKA-LQFIEDVTSNADEVQRRVLAGILSRSAHVEYLQRHGLNGCTDQETFKKVVP 81

Query: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120
           VV+YE+LKP IDRIANGDASPILCS+P+SEFLTSSGTSGGERKLMPT EEEL+RRS LYS
Sbjct: 82  VVTYEDLKPEIDRIANGDASPILCSQPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 141

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQ +PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKSSHF DRPYD YT+Y
Sbjct: 142 LLMPVMNQCVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFMDRPYDPYTNY 201

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPN+TILC DSYQSMY Q LCGL  + QVLRVG+VFASGFIRA+KFL+ +W+LL NDIR
Sbjct: 202 TSPNETILCPDSYQSMYSQLLCGLYQNTQVLRVGAVFASGFIRALKFLQMNWLLLCNDIR 261

Query: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300
           +GT++P+I+D  VR++VM IL+P+P LAD IE EC K +W+GII RLWPNTKYIDVIVTG
Sbjct: 262 SGTLDPKITDSSVREAVMKILEPNPRLADFIEDECGKDSWSGIITRLWPNTKYIDVIVTG 321

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFG+NLNPLC P+EVSYTLIPTM Y+EFLP++
Sbjct: 322 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGLNLNPLCKPSEVSYTLIPTMCYYEFLPVN 381

Query: 361 RKM----------------SEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFK 420
           RK                 +++LVDLVDV+LG+EYELV+TTY+GLYRYRVGDILRV+GFK
Sbjct: 382 RKSGFNESFSKPVSLNDKENQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDILRVAGFK 441

Query: 421 NKAPQFNFVCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPG 480
           NKAPQFNFVCRKNV+LSID+DK DEVELQNAV  A+ NL+ ++ SL EYTSYADT TVPG
Sbjct: 442 NKAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNAANNLLSFDASLTEYTSYADTSTVPG 501

Query: 481 HYVIYWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFD 540
           HYV+YWEIS      IP  VFE+CCLT+EES NSVYRQGRVSDKSIGPLEIKI+E+GTFD
Sbjct: 502 HYVLYWEISLSRATPIPPSVFEDCCLTVEESFNSVYRQGRVSDKSIGPLEIKIVESGTFD 561

Query: 541 KLMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCP 570
            LMDYA+  G+SINQYK PRCVK+ PILELLNS VV+NYFSP+CP
Sbjct: 562 SLMDYAVGQGASINQYKAPRCVKYAPILELLNSKVVANYFSPKCP 605

BLAST of Carg13552-RA vs. TrEMBL
Match: tr|A0A1U7YQ53|A0A1U7YQ53_NELNU (indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC104585725 PE=4 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 3.9e-261
Identity = 435/577 (75.39%), Postives = 498/577 (86.31%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IE++TT+AD++Q+RVL EILSR+AHVEYLQ+ GL+G  DR  FK ++PVV+YE+L+ 
Sbjct: 31  LEFIEDVTTNADEIQKRVLAEILSRSAHVEYLQRHGLNGRTDRETFKKVMPVVTYEDLQS 90

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPILC  P+SEFLTSSGTS GERKLMPT EEEL+RRS LYSLLMPVM+QF
Sbjct: 91  DINRIANGDTSPILCGLPISEFLTSSGTSAGERKLMPTIEEELERRSLLYSLLMPVMDQF 150

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKS HFK RPYD YT+YTSPN+TILC
Sbjct: 151 VPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSLHFKHRPYDPYTNYTSPNETILC 210

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DSYQSMY Q LCGLC ++QVLRVG+VFASGFIRAI+FLE HW LL NDIRTG INP+I+
Sbjct: 211 PDSYQSMYSQLLCGLCQNKQVLRVGAVFASGFIRAIRFLEKHWSLLCNDIRTGAINPQIT 270

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           DP VR +V+ ILKPDP LAD IE EC++  W GII RLWPNTKYIDVIVTGTMSQYIPTL
Sbjct: 271 DPSVRDAVLRILKPDPELADFIESECTREAWQGIITRLWPNTKYIDVIVTGTMSQYIPTL 330

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP----------- 369
           DYYSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLP           
Sbjct: 331 DYYSNGLPLVCTMYASSECYFGLNLNPLCKPNEVSYTLIPTMAYFEFLPVHRNNGFTTSI 390

Query: 370 -----LDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                L+ K  ++LVDLVDV+LG+EYELV+TTY+GLYRYRVGD+LRV+GFKNKAPQFNFV
Sbjct: 391 SVPKSLNEKEQQDLVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNKAPQFNFV 450

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQ AV +A  +L+P++ SL+EYTSYADT T+PGHYV+YWE+ 
Sbjct: 451 CRKNVVLSIDSDKTDEVELQAAVKDAINHLVPFDASLIEYTSYADTTTIPGHYVLYWELR 510

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
              T  IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 511 HDSTTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAISR 570

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS V+SNYFSP+CPK
Sbjct: 571 GASINQYKTPRCVKFAPIVELLNSRVLSNYFSPKCPK 607

BLAST of Carg13552-RA vs. TrEMBL
Match: tr|B9T670|B9T670_RICCO (Indole-3-acetic acid-amido synthetase GH3.6, putative OS=Ricinus communis OX=3988 GN=RCOM_0032550 PE=4 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 5.7e-260
Identity = 432/577 (74.87%), Postives = 500/577 (86.66%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++T++ D+VQ++VL EIL+RNA VEYLQ+ GL+G  DR +FK I+PV++YE+++P
Sbjct: 24  LQFIEDVTSNPDEVQKKVLEEILTRNARVEYLQKHGLNGYTDRESFKKIMPVIAYEDIQP 83

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           +I+RIANGD SPILCS+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVMNQF
Sbjct: 84  HINRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLMPVMNQF 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKSSHFKDRP+D YT+YTSPN+TILC
Sbjct: 144 VPGLEKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFKDRPFDPYTNYTSPNETILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DSYQSMY Q LCGLC    VLRVG+VFASGFIRAI+FLE HW LL NDIRTGT+NP+I+
Sbjct: 204 PDSYQSMYSQMLCGLCQRNDVLRVGAVFASGFIRAIRFLEKHWQLLCNDIRTGTVNPQIT 263

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++VM ILKPDP LAD I  ECS+ +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 264 DLSVREAVMKILKPDPKLADFIAAECSQESWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 323

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP----------- 369
           DYYSNGLPLVCTMYASSECYFGVNLNPLC P+EVSYTLIPTMAYFEFLP           
Sbjct: 324 DYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVHRNNGVTNSI 383

Query: 370 -----LDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                L+ K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+LRV+GFKNKAPQFNF+
Sbjct: 384 SMPKSLNEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNKAPQFNFI 443

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQNAV  A  +L+P++ +L EYTSYADT ++PGHYV++WEI+
Sbjct: 444 CRKNVVLSIDSDKTDEVELQNAVKNAVNHLVPFDATLAEYTSYADTTSIPGHYVLFWEIT 503

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
              +  IP  +FE+CCLTIEESLNSVYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 504 LNGSTPIPPSIFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 563

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS VVS+YFSP+CPK
Sbjct: 564 GASINQYKTPRCVKFAPIVELLNSRVVSSYFSPKCPK 600

BLAST of Carg13552-RA vs. TrEMBL
Match: tr|A0A1R3KBU8|A0A1R3KBU8_9ROSI (GH3 auxin-responsive promoter OS=Corchorus olitorius OX=93759 GN=COLO4_09528 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 9.7e-260
Identity = 431/577 (74.70%), Postives = 499/577 (86.48%), Query Frame = 0

Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++T++AD+VQ++VL EILSRNAHVEYLQ  GL+G  DR  FK ++PV++YE+++P
Sbjct: 24  LQFIEDVTSNADEVQKKVLEEILSRNAHVEYLQNHGLNGHTDRETFKKVMPVITYEDIQP 83

Query: 70  YIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           +IDRIANGD SPILCS+P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 84  HIDRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLMPVMSQF 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKS+HFKDRPYD YT+YTSPN+TILC
Sbjct: 144 VPGLEKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSNHFKDRPYDPYTNYTSPNETILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIS 249
            DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAI+FLE HW LL NDIRTGT++ +I+
Sbjct: 204 PDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIRFLEKHWRLLCNDIRTGTLDAQIT 263

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPTL 309
           D  VR++VM ILKPDP LAD IE EC K +W GII RLWPNTKY+DVIVTGTMSQYIPTL
Sbjct: 264 DQSVREAVMTILKPDPKLADFIEAECGKDSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 323

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP----------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTL+PTMAYFEFLP           
Sbjct: 324 DYYSNGLPLVCTMYASSECYFGVNLKPLCKPSEVSYTLVPTMAYFEFLPVHRNNGVTNSI 383

Query: 370 -----LDRKMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNFV 429
                L+ K  +ELVDLVDV+LG+EYELV+TTY+GLYRYRVGD+LRV+GFKNKAPQFNF+
Sbjct: 384 SVPKSLNEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLRVAGFKNKAPQFNFI 443

Query: 430 CRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEIS 489
           CRKNV+LSID+DK DEVELQ+AV  A  +L+P++ +L EYTSYADT ++PGHYV++WE+S
Sbjct: 444 CRKNVVLSIDSDKTDEVELQSAVKNAVNHLLPFDATLAEYTSYADTTSIPGHYVLFWELS 503

Query: 490 QKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 549
              T  IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ 
Sbjct: 504 LDGTTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISL 563

Query: 550 GSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
           G+SINQYKTPRCVK  PI+ELLNS  VS+YFSP+CPK
Sbjct: 564 GASINQYKTPRCVKFAPIVELLNSRAVSSYFSPKCPK 600

BLAST of Carg13552-RA vs. TrEMBL
Match: tr|A0A1U8NWP7|A0A1U8NWP7_GOSHI (indole-3-acetic acid-amido synthetase GH3.6-like OS=Gossypium hirsutum OX=3635 GN=LOC107951777 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 9.7e-260
Identity = 433/578 (74.91%), Postives = 496/578 (85.81%), Query Frame = 0

Query: 9   LLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLK 68
           +LQ IE++T++AD+VQ++VL EILSRNAHVEYLQ  GL+G  DR  FK I+PV++YE+++
Sbjct: 24  ILQFIEDVTSNADEVQKKVLEEILSRNAHVEYLQTHGLNGHTDRETFKTIMPVITYEDIQ 83

Query: 69  PYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYSLLMPVMNQ 128
           PY+DRIANGD SPIL S P+SEFLTSSGTSGGERKLMPT EEEL RRS LYSLLMPVM+Q
Sbjct: 84  PYVDRIANGDTSPILSSHPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLMPVMSQ 143

Query: 129 FIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTIL 188
           F+PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKS+HFKDRPYD YT+YTSPN+TIL
Sbjct: 144 FVPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSTHFKDRPYDPYTNYTSPNETIL 203

Query: 189 CTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRI 248
           C DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  L NDIRTGTI+ +I
Sbjct: 204 CPDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWRFLCNDIRTGTIDSQI 263

Query: 249 SDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTGTMSQYIPT 308
           +D  VR++VM ILKPDP LAD IE ECSK +W GII RLWPNTKY+DVIVTGTMSQYIPT
Sbjct: 264 TDQSVREAVMKILKPDPQLADFIEAECSKDSWQGIITRLWPNTKYVDVIVTGTMSQYIPT 323

Query: 309 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR------- 368
           LDYY+NGLPLVCTMYASSECYFGVNLNPLC P+EVSYTLIPTMAYFEFLP+ R       
Sbjct: 324 LDYYTNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLPVQRNNGVNSS 383

Query: 369 ---------KMSEELVDLVDVELGKEYELVITTYSGLYRYRVGDILRVSGFKNKAPQFNF 428
                    K  +ELVDLVDV+LG+EYELV+TTYSGLYRYRVGD+LRV+GFKN APQFNF
Sbjct: 384 ISVPKTLNEKEQQELVDLVDVKLGQEYELVVTTYSGLYRYRVGDVLRVAGFKNNAPQFNF 443

Query: 429 VCRKNVILSIDTDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEI 488
           +CRKNV+L ID+DK DEVELQNAV  A  + +P++ +L EYTSYADT T+PGHYV+YWE+
Sbjct: 444 ICRKNVVLCIDSDKTDEVELQNAVKNAVNHFLPFDATLAEYTSYADTTTIPGHYVLYWEL 503

Query: 489 SQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAIN 548
           S   T  IP  VFE+CCLTIEESLNSVYRQGRV DKSIGPLEIKI+E GTFDKLMDYAI+
Sbjct: 504 SLNGTTPIPPSVFEDCCLTIEESLNSVYRQGRVCDKSIGPLEIKIVEPGTFDKLMDYAIS 563

Query: 549 HGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPK 571
            G+SINQYKTPRCVK  PI+ELLNS  VS++FSP+CPK
Sbjct: 564 LGASINQYKTPRCVKFAPIVELLNSRAVSSFFSPKCPK 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948165.10.0e+0099.65indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata][more]
XP_023533206.10.0e+0098.42indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo][more]
XP_022970970.10.0e+0097.89indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima][more]
XP_022140599.15.0e-28482.79indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 [Momordica charantia... [more]
XP_023901431.12.0e-26174.70indole-3-acetic acid-amido synthetase GH3.6-like [Quercus suber] >POE49631.1 ind... [more]
Match NameE-valueIdentityDescription
AT5G54510.11.2e-25874.18Auxin-responsive GH3 family protein[more]
AT4G27260.16.6e-25472.62Auxin-responsive GH3 family protein[more]
AT4G37390.17.1e-21663.81Auxin-responsive GH3 family protein[more]
AT2G14960.13.9e-21464.41Auxin-responsive GH3 family protein[more]
AT2G23170.18.7e-21464.22Auxin-responsive GH3 family protein[more]
Match NameE-valueIdentityDescription
sp|Q9LSQ4|GH36_ARATH2.1e-25774.18Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
sp|O81829|GH35_ARATH1.2e-25272.62Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
sp|Q8LQM5|GH31_ORYSJ4.1e-22165.14Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
sp|A3BLS0|GH38_ORYSI2.1e-21764.90Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indi... [more]
sp|Q0D4Z6|GH38_ORYSJ2.1e-21764.90Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
tr|A0A2P4H061|A0A2P4H061_QUESU1.4e-26174.70Indole-3-acetic acid-amido synthetase gh3.6 OS=Quercus suber OX=58331 GN=CFP56_0... [more]
tr|A0A1U7YQ53|A0A1U7YQ53_NELNU3.9e-26175.39indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC10... [more]
tr|B9T670|B9T670_RICCO5.7e-26074.87Indole-3-acetic acid-amido synthetase GH3.6, putative OS=Ricinus communis OX=398... [more]
tr|A0A1R3KBU8|A0A1R3KBU8_9ROSI9.7e-26074.70GH3 auxin-responsive promoter OS=Corchorus olitorius OX=93759 GN=COLO4_09528 PE=... [more]
tr|A0A1U8NWP7|A0A1U8NWP7_GOSHI9.7e-26074.91indole-3-acetic acid-amido synthetase GH3.6-like OS=Gossypium hirsutum OX=3635 G... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR004993GH3
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg13552Carg13552gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg13552-RACarg13552-RA-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13552-RA.CDS.3Carg13552-RA.CDS.3CDS
Carg13552-RA.CDS.2Carg13552-RA.CDS.2CDS
Carg13552-RA.CDS.1Carg13552-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13552-RA.exon.3Carg13552-RA.exon.3exon
Carg13552-RA.exon.2Carg13552-RA.exon.2exon
Carg13552-RA.exon.1Carg13552-RA.exon.1exon


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 13..548
e-value: 6.6E-204
score: 678.5
IPR004993GH3 familyPANTHERPTHR31901FAMILY NOT NAMEDcoord: 8..570
NoneNo IPR availablePANTHERPTHR31901:SF2INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.5-RELATEDcoord: 8..570