Carg10813 (gene) Silver-seed gourd

Overview
NameCarg10813
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPhospholipase A2
LocationCucurbita_argyrosperma_scaffold_034 : 274877 .. 278100 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGAAGAGTTCATATCGCAGAGCCAATCGGACGGGAGAGACGCGGTACAGAAAAGCTCCGCCAAAGGAATCTGTTTGTGGTCTGAAGAAGGCGACTGAAGATGGTCTGCAATACCAGCATTTCCCTTCGCAAACAAGTCGCATCGGCGATTTTCCTTCTCCTCGTTCTACTCACCGTCGTCTCCGAGTGTTCCAGTCAGGTAATAATCTGCTCCTTTTGTTATTTTAAGAACTGATGCTGGAGAATTTGGATCTTAGATTCGTACATTTGGTTCTTCAGTCTCCTTCGTTTGTTTCTTGATGTGAAATTCAATTTGTTTGGCTTCTATTCAACGATTTGGATGTTAACGTCAAATTTTCGTTGGATGATAGGTTGAATGCAGTAGAACTTGTGCTTCAGAGAAATGCAATAGTGAGTATATTCTGTGTTTTTATATGAAATTCGTCATCTGTTGTTATTATTTTTTGAATTTTAATTTCTCATCAAATATTTATTTTGTTTGTTTATCTGAAAATTAGCTGTAGGGATTCGGTACGGTAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGAGCTTGATGCTTGTTGCAAAGATCATGACGAATGTGTTGAATATAAAGGTTATTGCCAATCTATAACTACATGCCTTATTGTTTCCTTTCTATACTTCGCACTGTTTGCGATTCCTCATCATTATACTTGCATAGAAAAATTTCCCATCGATCTATATTCCTGTTTTAGTTTTAAGTTGTTGTGAGACCAATGGGGTGAAATCTGGGCATCCTGTTGTTCAATGAACTCTTTTTTCGGGCATTATTGGTATATTCTCTGCTGCATGCTTGGAAAAAGATACGTATGAGATTCAAATATTCGACTACGATTTCTACCCTTTGAGAATAGAAGAATCGAAATTTGCTTTCCCAACAAGATCTTTCTCAATGAGGCTACCCCTTGTTTTCATGCCCCTTAAATCATTTCCGTTCTCAAATAAATTCCCGTTGAATACTTCATCCTCGGCCTCAAATTTAATTTAAAAAGGGCGACAATCCTCGCATCTTGACTGAACTCAGCGGGTCGAACCATTCACATGACTCCTCGATAGTATGATAATGTCCACTTTGGATATAAGTCTCATACCAATGGAGATGGTTTTCTTTATTTTCCCATGATCTTCCCCCTTCCGTAGTCAATGTGAACCTTTGGTCGCACTTTCTACAATCTTCCACCAAATAAAGTGCCATGGAGCCTCCCCGTTCTAGGGCTCACCATCTGTTTGTTTGGCATCCAAAGACTCTTTCAACATGGCTAAGTCAAGAAGGTGATTATGATACCATTACAAGAGTTAGAAAGAGAGAGAAGAGAATGTGATCATAAAAGTACTCCGCTGTTCAACATTAGTGGTTGGAGAAGTAATCCTATTTATAGGACTCTCTGAAACAATATTAACTTACGGTACGACTGTATACTAAAGCAGTATTAGCAAAGATACATCGTATCAAGCTCTTAACAACCAATGAGTTAAGTCTTAACACCTTTGGCTTCTAAATTCTTAAACAATGTCATCAAACTCCAAGCCACCAGTTTCTCCCTACCAACATACATCATCAATGGATCCTAACTAAACCTAACCACGGAGAATAGCTACTTCCATTCGAGGAAACTGTTGTCCTATAGTTTAGTCCCGACTTGTGAGCTGCTGGGTTGGCCACATTAATAATGTTGGAGAGGTTAATGAGTTGTTTTTCTAAACCCGAGTCCTCCCAGTTGAATTTTCTTATGGATGATGTACTGCCATTTCCTCTGTCATATGCTTACCTGTGAGAAGCTTCATCAGATTCTTGATACCTCAAGGTTAGGGTGCTTCCTTATACTGCCAGCGTGTAGAGGCTGATTACCATCCTCAAAATGCTTATGCGGTATAATAAACACTTTTGGTTGTTATTAGATTTTTTTGACAGAAGACAAAGAATGGTTGAATAGTCTATTGTGTTCACAACTTTTAAGTCGAATCCTTCGTGTCGTTAACTTCGGAGTAGGATGACAAACTGTCACATGTTAACTCCCTTTTCAAATGCATGGCAGTAAGGACGTTGAACACTTTTGCCTCCCTTGTAGTGATCATTGAGTGGATTACATCAGTAAATGCCTACGAATAGTCAGTTATTTATATTATGCACATAGTAGACTTCTGTTTATTACGGATCAGTAAAGCTATTGGCCTCCTGGCCATTGAAAAAAAAAATTAGATACAAAATTGGAGATATTAAGGCATGGCCAATCGGCATATTAAAACAAAGAGGAGACACTAGACAGTCACCAATGTTTGGTGTTGCTAAAGTCTATATCTTAGATCTTAGATGTCTTTTATTTATCTGAGATACTACACATTTCAAACTTTGTAGCACTAGATTACAGGAGACCAAGCAATTTACATATATATATACATATATTGGTTCATTTTTGCAATATTGTTGTACCATGTTTTCTTTTTGTTCAATTATGATTTTGGGTGGAATTTTAAGAGTTGGAACTTGAGAGTCACTCTGTTGAATATGTTTTGTTTCAAGACCAATAACAGATATCACATGGGTTTTTCAGGTTTAACCAATATTAAATGTCACATAAAGTTCAAGGATTGTATTACGAAATTGCAGAAATCTGGGATGGCTGGTTTCTCACAGCAGTGCCCTTATTCCATAGCTATTCCTACAATGGTAAATGGCATGGATATAGCCATCATGTTCAGCCAGTTTAGTAGCGGAAAGTTTGAGCTCTGATAGCCACTTTGGTAACTCAACTCGCTCCATCATCCCCGCCCACAATTCATGCCATCAATTATATTGCTCATACTCCCCCAAAACAAGGTGGATAGCTTCACACCACTGATTCATTCTTTTAAATAATATTCTATTGGATTAAGAAATCTCAAACAGAGGATTTGATCCTCATTTTTTTGTCCCCTTTAACTGTTATTTGGACCATCTGCACTTTAAGGATGTTTTTAGCATAGTGAAAGAAGATTCCAATAGGCCAAATTATTTCATGTTTGATGCTTCCTCAATAATCAATAGCTGTTAGGTTTTGGTTTCCCTCCTTATTGTGTGTCAAATACTCCATTTTTAAGGAGTCACCCAATGTGTGGGCAGACTGTTGCAAGTTATCTTCTGTTGTTGCCTTTTCGTTTGAAGTTGAACAATCACAGTTTA

mRNA sequence

ACGAAGAGTTCATATCGCAGAGCCAATCGGACGGGAGAGACGCGGTACAGAAAAGCTCCGCCAAAGGAATCTGTTTGTGGTCTGAAGAAGGCGACTGAAGATGGTCTGCAATACCAGCATTTCCCTTCGCAAACAAGTCGCATCGGCGATTTTCCTTCTCCTCGTTCTACTCACCGTCGTCTCCGAGTGTTCCAGTCAGGTTGAATGCAGTAGAACTTGTGCTTCAGAGAAATGCAATACTGTAGGGATTCGGTACGGTAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGAGCTTGATGCTTGTTGCAAAGATCATGACGAATGTGTTGAATATAAAGGTTTAACCAATATTAAATGTCACATAAAGTTCAAGGATTGTATTACGAAATTGCAGAAATCTGGGATGGCTGGTTTCTCACAGCAGTGCCCTTATTCCATAGCTATTCCTACAATGGTAAATGGCATGGATATAGCCATCATGTTCAGCCAGTTTAGTAGCGGAAAGTTTGAGCTCTGATAGCCACTTTGGTAACTCAACTCGCTCCATCATCCCCGCCCACAATTCATGCCATCAATTATATTGCTCATACTCCCCCAAAACAAGGTGGATAGCTTCACACCACTGATTCATTCTTTTAAATAATATTCTATTGGATTAAGAAATCTCAAACAGAGGATTTGATCCTCATTTTTTTGTCCCCTTTAACTGTTATTTGGACCATCTGCACTTTAAGGATGTTTTTAGCATAGTGAAAGAAGATTCCAATAGGCCAAATTATTTCATGTTTGATGCTTCCTCAATAATCAATAGCTGTTAGGTTTTGGTTTCCCTCCTTATTGTGTGTCAAATACTCCATTTTTAAGGAGTCACCCAATGTGTGGGCAGACTGTTGCAAGTTATCTTCTGTTGTTGCCTTTTCGTTTGAAGTTGAACAATCACAGTTTA

Coding sequence (CDS)

ATGGTCTGCAATACCAGCATTTCCCTTCGCAAACAAGTCGCATCGGCGATTTTCCTTCTCCTCGTTCTACTCACCGTCGTCTCCGAGTGTTCCAGTCAGGTTGAATGCAGTAGAACTTGTGCTTCAGAGAAATGCAATACTGTAGGGATTCGGTACGGTAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGAGCTTGATGCTTGTTGCAAAGATCATGACGAATGTGTTGAATATAAAGGTTTAACCAATATTAAATGTCACATAAAGTTCAAGGATTGTATTACGAAATTGCAGAAATCTGGGATGGCTGGTTTCTCACAGCAGTGCCCTTATTCCATAGCTATTCCTACAATGGTAAATGGCATGGATATAGCCATCATGTTCAGCCAGTTTAGTAGCGGAAAGTTTGAGCTCTGA

Protein sequence

MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIAIMFSQFSSGKFEL
Homology
[Close]
BLAST of Carg10813 vs. NCBI nr
Match: XP_022946468.1 (probable phospholipase A2 homolog 1 [Cucurbita moschata])

HSP 1 Score: 300.8 bits (769), Expect = 2.5e-78
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0 

Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQV+CSRTCASEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVQCSRTCASEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]
BLAST of Carg10813 vs. NCBI nr
Match: XP_022999541.1 (probable phospholipase A2 homolog 1 [Cucurbita maxima])

HSP 1 Score: 295.8 bits (756), Expect = 7.9e-77
Identity = 141/145 (97.24%), Postives = 143/145 (98.62%), Query Frame = 0 

Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRK VASAIFLLLVLL+VVSECSSQVECSRTC SEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKHVASAIFLLLVLLSVVSECSSQVECSRTCVSEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCD+LDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDDLDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]
BLAST of Carg10813 vs. NCBI nr
Match: XP_023545532.1 (probable phospholipase A2 homolog 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.4 bits (747), Expect = 8.7e-76
Identity = 140/145 (96.55%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCN SISLRK VASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNISISLRKHVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCH KFK CITK+QKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHTKFKSCITKVQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]
BLAST of Carg10813 vs. NCBI nr
Match: XP_022154742.1 (probable phospholipase A2 homolog 1 [Momordica charantia] >XP_022154743.1 probable phospholipase A2 homolog 1 [Momordica charantia])

HSP 1 Score: 219.5 bits (558), Expect = 7.2e-54
Identity = 104/148 (70.27%), Postives = 120/148 (81.08%), Query Frame = 0 

Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECS---SQVECSRTCASEKCNTVGIRYGKFCG 60
           MV + SI +RK  +S   L+L+ L+VVSECS   S+VECSRTC +  CNTVGIRYGKFCG
Sbjct: 1   MVRHPSIPIRKHFSSPFLLVLLFLSVVSECSSNESRVECSRTCVAINCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCP 120
           VGWTGCAGEKPCD+LDACCK HDECVE KGLTNIKCH KFK CI K+QKSG AGFS++CP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKSCIKKVQKSGKAGFSEECP 120

Query: 121 YSIAIPTMVNGMDIAIMFSQFSSGKFEL 146
           YS A+P MV GMD+AI+FSQ  + K EL
Sbjct: 121 YSTAVPIMVQGMDLAIVFSQLGNSKLEL 148

[Close]
BLAST of Carg10813 vs. NCBI nr
Match: XP_008459246.1 (PREDICTED: probable phospholipase A2 homolog 1 [Cucumis melo])

HSP 1 Score: 206.5 bits (524), Expect = 6.3e-50
Identity = 92/121 (76.03%), Postives = 103/121 (85.12%), Query Frame = 0 

Query: 28  SECS---SQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVE 87
           S+CS   S+VECSRTC +  CNTVGIRYGKFCGVGWTGCAGEKPCD+LDACCK HDECVE
Sbjct: 20  SDCSNNESRVECSRTCVAVNCNTVGIRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVE 79

Query: 88  YKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIAIMFSQFSSGKFE 146
            KGLTN+KCH KFK CI K+QKSG  GFS++CPYS A+PTMV GMD+AIMFSQF + K E
Sbjct: 80  RKGLTNVKCHEKFKSCIKKVQKSGKVGFSKECPYSTAVPTMVQGMDLAIMFSQFGNSKLE 139

[Close]
BLAST of Carg10813 vs. TAIR10
Match: AT2G19690.2 (phospholipase A2-beta)

HSP 1 Score: 150.6 bits (379), Expect = 7.5e-37
Identity = 67/131 (51.15%), Postives = 92/131 (70.23%), Query Frame = 0 

Query: 15  SAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDA 74
           +A F  +V + +V    S+ EC+RTC ++ C+T+ IRYGK+CG+G +GC GE+PCD+LDA
Sbjct: 11  AAAFFAIVFVVLVGVARSE-ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDA 70

Query: 75  CCKDHDECVEYKGLTNIKCHIKFKDCITKL------QKSGMAGFSQQCPYSIAIPTMVNG 134
           CCK HD CVE  G+TNI CH KF+ C+ +L       K+   GFS +CPYS+ IPT+  G
Sbjct: 71  CCKIHDHCVELNGMTNISCHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQG 130

Query: 135 MDIAIMFSQFS 140
           MDI I+FSQ +
Sbjct: 131 MDIGILFSQLA 140

[Close]
BLAST of Carg10813 vs. TAIR10
Match: AT4G29460.1 (Phospholipase A2 family protein)

HSP 1 Score: 150.6 bits (379), Expect = 7.5e-37
Identity = 69/121 (57.02%), Postives = 85/121 (70.25%), Query Frame = 0 

Query: 22  VLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDE 81
           +LL VV   SSQ +CS TC ++ CN++GIRYGK+CG+G+ GC GE PCD+LDACC  HD 
Sbjct: 18  LLLAVV---SSQEKCSNTCIAQNCNSLGIRYGKYCGIGYFGCPGEPPCDDLDACCMTHDN 77

Query: 82  CVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAIPTMVNGMDIAIMF 137
           CV+ KG+T + CH +FK C+ KL KS         GFS QCPYSI IPT+ NGMD  I F
Sbjct: 78  CVDLKGMTYVNCHKQFKRCVNKLSKSIKHSNGEKIGFSTQCPYSIVIPTVFNGMDYGIFF 135

[Close]
BLAST of Carg10813 vs. TAIR10
Match: AT4G29470.1 (Phospholipase A2 family protein)

HSP 1 Score: 137.5 bits (345), Expect = 6.5e-33
Identity = 60/134 (44.78%), Postives = 89/134 (66.42%), Query Frame = 0 

Query: 9   LRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKP 68
           +R    + + L L +  +++   SQ +CS+TC ++KCN +GIRYGK+CG+G+ GC GE P
Sbjct: 2   IRGGALTHVALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLGIRYGKYCGIGYFGCPGEPP 61

Query: 69  CDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAI 128
           CD+LD CC  HD CV+ KG+T + CH +F+ C+ +L++S         GFS++CPYS  I
Sbjct: 62  CDDLDDCCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKECPYSTVI 121

Query: 129 PTMVNGMDIAIMFS 137
           PT+  GM+  I FS
Sbjct: 122 PTVYRGMNYGIFFS 135

[Close]
BLAST of Carg10813 vs. TAIR10
Match: AT2G06925.1 (Phospholipase A2 family protein)

HSP 1 Score: 77.4 bits (189), Expect = 8.0e-15
Identity = 39/104 (37.50%), Postives = 58/104 (55.77%), Query Frame = 0 

Query: 35  ECSRTCASEKCNTVG-IRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKG--LTNI 94
           ECSR C SE C+    +RYGK+CG+ ++GC GE+PCD LD+CC  HD CV+ K     + 
Sbjct: 37  ECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDSCCMKHDACVQSKNNDYLSQ 96

Query: 95  KCHIKFKDCITKLQKSGMAGF-SQQCPYSIAIPTMVNGMDIAIM 135
           +C  KF +C+    +     F   +C     I  +   M+ A++
Sbjct: 97  ECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVMEAALI 140

[Close]
BLAST of Carg10813 vs. Swiss-Prot
Match: sp|Q9XG80|PLA21_ORYSJ (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 6.9e-40
Identity = 67/109 (61.47%), Postives = 91/109 (83.49%), Query Frame = 0 

Query: 37  SRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIK 96
           SR+CA+  C++VGIRYGK+CGVGW+GC GE+PCD+LDACC+DHD CV+ KGL ++KCH K
Sbjct: 30  SRSCAALNCDSVGIRYGKYCGVGWSGCDGEEPCDDLDACCRDHDHCVDKKGLMSVKCHEK 89

Query: 97  FKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIAIMFSQFSSGKFEL 146
           FK+C+ K++K+G  GFS++CPY +A+ TM +GMD+AIM SQ  + K EL
Sbjct: 90  FKNCMRKVKKAGKIGFSRKCPYEMAMATMTSGMDMAIMLSQLGTQKLEL 138

[Close]
BLAST of Carg10813 vs. Swiss-Prot
Match: sp|Q8GZB4|PLA2B_ARATH (Phospholipase A2-beta OS=Arabidopsis thaliana OX=3702 GN=PLA2-BETA PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.3e-35
Identity = 67/132 (50.76%), Postives = 92/132 (69.70%), Query Frame = 0 

Query: 15  SAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDA 74
           +A F  +V + +V    S+ EC+RTC ++ C+T+ IRYGK+CG+G +GC GE+PCD+LDA
Sbjct: 11  AAAFFAIVFVVLVGVARSE-ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDA 70

Query: 75  CCKDHDECVEYKGLTNIKCHIKFKDCITKL------QKSGMAGFSQQCPYSIAIPTMVNG 134
           CCK HD CVE  G+TNI CH KF+ C+ +L       K+   GFS +CPYS+ IPT+  G
Sbjct: 71  CCKIHDHCVELNGMTNISCHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQG 130

Query: 135 MDIAIMFSQFSS 141
           MDI I+FSQ  +
Sbjct: 131 MDIGILFSQLGN 141

[Close]
BLAST of Carg10813 vs. Swiss-Prot
Match: sp|Q9M0D7|PLA2C_ARATH (Phospholipase A2-gamma OS=Arabidopsis thaliana OX=3702 GN=PLA2-GAMMA PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.3e-35
Identity = 69/121 (57.02%), Postives = 85/121 (70.25%), Query Frame = 0 

Query: 22  VLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDE 81
           +LL VV   SSQ +CS TC ++ CN++GIRYGK+CG+G+ GC GE PCD+LDACC  HD 
Sbjct: 18  LLLAVV---SSQEKCSNTCIAQNCNSLGIRYGKYCGIGYFGCPGEPPCDDLDACCMTHDN 77

Query: 82  CVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAIPTMVNGMDIAIMF 137
           CV+ KG+T + CH +FK C+ KL KS         GFS QCPYSI IPT+ NGMD  I F
Sbjct: 78  CVDLKGMTYVNCHKQFKRCVNKLSKSIKHSNGEKIGFSTQCPYSIVIPTVFNGMDYGIFF 135

[Close]
BLAST of Carg10813 vs. Swiss-Prot
Match: sp|Q8GV50|PLA2D_ARATH (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-31
Identity = 60/134 (44.78%), Postives = 89/134 (66.42%), Query Frame = 0 

Query: 9   LRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKP 68
           +R    + + L L +  +++   SQ +CS+TC ++KCN +GIRYGK+CG+G+ GC GE P
Sbjct: 2   IRGGALTHVALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLGIRYGKYCGIGYFGCPGEPP 61

Query: 69  CDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAI 128
           CD+LD CC  HD CV+ KG+T + CH +F+ C+ +L++S         GFS++CPYS  I
Sbjct: 62  CDDLDDCCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKECPYSTVI 121

Query: 129 PTMVNGMDIAIMFS 137
           PT+  GM+  I FS
Sbjct: 122 PTVYRGMNYGIFFS 135

[Close]
BLAST of Carg10813 vs. Swiss-Prot
Match: sp|Q10E50|PLA23_ORYSJ (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 6.9e-16
Identity = 36/83 (43.37%), Postives = 53/83 (63.86%), Query Frame = 0 

Query: 36  CSRTCASEKCNTVG-IRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKG-LTNIKC 95
           CSRTC S+ C T   +RYGK+CG+ ++GC GE+PCDELDACC  HD CV+ K    +  C
Sbjct: 55  CSRTCESDHCTTPPLLRYGKYCGILYSGCPGEQPCDELDACCMHHDNCVQAKNDYLSTAC 114

Query: 96  HIKFKDCITKLQKSGMAGFSQQC 117
           + +  +C+ +L++        +C
Sbjct: 115 NEELLECLARLREGSSTFQGNKC 137

[Close]
BLAST of Carg10813 vs. TrEMBL
Match: tr|A0A1S3C986|A0A1S3C986_CUCME (probable phospholipase A2 homolog 1 OS=Cucumis melo OX=3656 GN=LOC103498425 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.2e-50
Identity = 92/121 (76.03%), Postives = 103/121 (85.12%), Query Frame = 0 

Query: 28  SECS---SQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVE 87
           S+CS   S+VECSRTC +  CNTVGIRYGKFCGVGWTGCAGEKPCD+LDACCK HDECVE
Sbjct: 20  SDCSNNESRVECSRTCVAVNCNTVGIRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVE 79

Query: 88  YKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIAIMFSQFSSGKFE 146
            KGLTN+KCH KFK CI K+QKSG  GFS++CPYS A+PTMV GMD+AIMFSQF + K E
Sbjct: 80  RKGLTNVKCHEKFKSCIKKVQKSGKVGFSKECPYSTAVPTMVQGMDLAIMFSQFGNSKLE 139

[Close]
BLAST of Carg10813 vs. TrEMBL
Match: tr|A0A0A0LFR9|A0A0A0LFR9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000560 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.3e-48
Identity = 90/121 (74.38%), Postives = 100/121 (82.64%), Query Frame = 0 

Query: 28  SECS---SQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVE 87
           S CS   S+VECSRTC +  CNTVGIRYGKFCGVGWTGCAGEKPCD+LDACCK HDECVE
Sbjct: 20  SHCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVE 79

Query: 88  YKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIAIMFSQFSSGKFE 146
            KGLTN+KCH KFK CI K+QKSG  GFS  CPYS A+PTMV GM++AIMFS+F + K E
Sbjct: 80  RKGLTNVKCHEKFKSCIKKVQKSGKVGFSNDCPYSTAVPTMVQGMNLAIMFSKFGNSKLE 139

[Close]
BLAST of Carg10813 vs. TrEMBL
Match: tr|W9QS24|W9QS24_9ROSA (Putative phospholipase A2-1-like protein OS=Morus notabilis OX=981085 GN=L484_027241 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 3.9e-48
Identity = 90/142 (63.38%), Postives = 111/142 (78.17%), Query Frame = 0 

Query: 7   ISLRKQVASAIFLLLVLLTVVSECS---SQVECSRTCASEKCNTVGIRYGKFCGVGWTGC 66
           + +R +VA+   ++L+ LTVV+ CS   S+V CS+ C +E CN+VGIRYGKFCGVGWTGC
Sbjct: 8   VPVRTRVAAGFAVVLISLTVVAHCSNNDSEVRCSKICVAENCNSVGIRYGKFCGVGWTGC 67

Query: 67  AGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIP 126
            GEKPCD+LDACCK HDECV  KGLTN+KCH KFK CI ++QKSG  GFS+ CPY  A+P
Sbjct: 68  PGEKPCDDLDACCKIHDECVTKKGLTNVKCHEKFKSCIKRVQKSGKVGFSRVCPYKTAVP 127

Query: 127 TMVNGMDIAIMFSQFSSGKFEL 146
           TMV GMD+AI+ SQF + K EL
Sbjct: 128 TMVQGMDLAILLSQFGNSKAEL 149

[Close]
BLAST of Carg10813 vs. TrEMBL
Match: tr|A0A2N9J591|A0A2N9J591_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60509 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 3.9e-48
Identity = 89/143 (62.24%), Postives = 110/143 (76.92%), Query Frame = 0 

Query: 7   ISLRKQVASAIFLLLVLLTVVSECS----SQVECSRTCASEKCNTVGIRYGKFCGVGWTG 66
           I +R +VA+    +   LTV++ CS    S+  CSRTC +E CN+VGIRYGK+CGVGWTG
Sbjct: 9   IPVRTRVAAVFAFVFTFLTVIAHCSNINNSETTCSRTCVAENCNSVGIRYGKYCGVGWTG 68

Query: 67  CAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAI 126
           C GEKPCD+LDACCK HDECV Y G+ N+KCH KFK CI ++QKSG AGFS +CPY  A+
Sbjct: 69  CPGEKPCDDLDACCKIHDECVGYHGVANVKCHEKFKKCIKRVQKSGKAGFSWECPYDTAV 128

Query: 127 PTMVNGMDIAIMFSQFSSGKFEL 146
           PTMV GMD+AI+ SQF++GK EL
Sbjct: 129 PTMVQGMDLAILMSQFATGKVEL 151

[Close]
BLAST of Carg10813 vs. TrEMBL
Match: tr|A0A1U8Q7E1|A0A1U8Q7E1_NELNU (probable phospholipase A2 homolog 1 isoform X1 OS=Nelumbo nucifera OX=4432 GN=LOC104604414 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.5e-47
Identity = 89/133 (66.92%), Postives = 108/133 (81.20%), Query Frame = 0 

Query: 14  ASAIFLLLVLLTVVSECSS-QVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDEL 73
           A  I +   +L VV ECS+ QV+CS+TC ++ CN+VGIRYGKFCGVGWTGC GEKPCD+L
Sbjct: 10  ARFIVVFSCVLLVVVECSNPQVKCSKTCVADNCNSVGIRYGKFCGVGWTGCPGEKPCDDL 69

Query: 74  DACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIA 133
           DACCK HDECVE KGL ++KCH KFK CI ++QKSG  GFS+QCPY IA+PTM+ GMD+A
Sbjct: 70  DACCKTHDECVEKKGLMSVKCHEKFKSCIKRVQKSGKIGFSRQCPYEIAMPTMIQGMDMA 129

Query: 134 IMFSQFSSGKFEL 146
           I+FSQF + K EL
Sbjct: 130 ILFSQFGNQKLEL 142

The following BLAST results are available for this feature:
BLAST of Carg10813 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022946468.12.5e-7899.31probable phospholipase A2 homolog 1 [Cucurbita moschata][more]
XP_022999541.17.9e-7797.24probable phospholipase A2 homolog 1 [Cucurbita maxima][more]
XP_023545532.18.7e-7696.55probable phospholipase A2 homolog 1 [Cucurbita pepo subsp. pepo][more]
XP_022154742.17.2e-5470.27probable phospholipase A2 homolog 1 [Momordica charantia] >XP_022154743.1 probab... [more]
XP_008459246.16.3e-5076.03PREDICTED: probable phospholipase A2 homolog 1 [Cucumis melo][more]
BLAST of Carg10813 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G19690.27.5e-3751.15phospholipase A2-beta[more]
AT4G29460.17.5e-3757.02Phospholipase A2 family protein[more]
AT4G29470.16.5e-3344.78Phospholipase A2 family protein[more]
AT2G06925.18.0e-1537.50Phospholipase A2 family protein[more]
BLAST of Carg10813 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9XG80|PLA21_ORYSJ6.9e-4061.47Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
sp|Q8GZB4|PLA2B_ARATH1.3e-3550.76Phospholipase A2-beta OS=Arabidopsis thaliana OX=3702 GN=PLA2-BETA PE=1 SV=1[more]
sp|Q9M0D7|PLA2C_ARATH1.3e-3557.02Phospholipase A2-gamma OS=Arabidopsis thaliana OX=3702 GN=PLA2-GAMMA PE=1 SV=1[more]
sp|Q8GV50|PLA2D_ARATH1.2e-3144.78Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1[more]
sp|Q10E50|PLA23_ORYSJ6.9e-1643.37Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III ... [more]
BLAST of Carg10813 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3C986|A0A1S3C986_CUCME4.2e-5076.03probable phospholipase A2 homolog 1 OS=Cucumis melo OX=3656 GN=LOC103498425 PE=3... [more]
tr|A0A0A0LFR9|A0A0A0LFR9_CUCSA2.3e-4874.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000560 PE=3 SV=1[more]
tr|W9QS24|W9QS24_9ROSA3.9e-4863.38Putative phospholipase A2-1-like protein OS=Morus notabilis OX=981085 GN=L484_02... [more]
tr|A0A2N9J591|A0A2N9J591_FAGSY3.9e-4862.24Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60509 PE=3 SV=1[more]
tr|A0A1U8Q7E1|A0A1U8Q7E1_NELNU1.5e-4766.92probable phospholipase A2 homolog 1 isoform X1 OS=Nelumbo nucifera OX=4432 GN=LO... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0016042lipid catabolic process
GO:0006644phospholipid metabolic process
GO:0050482arachidonic acid secretion
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO:0004623phospholipase A2 activity
Vocabulary: INTERPRO
TermDefinition
IPR033113PLipase_A2_His_AS
IPR001211PLipase_A2
IPR036444PLipase_A2_dom_sf
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050482 arachidonic acid secretion
biological_process GO:0009395 phospholipid catabolic process
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006644 phospholipid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004623 phospholipase A2 activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10813-RACarg10813-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036444Phospholipase A2 domain superfamilyGENE3DG3DSA:1.20.90.10coord: 20..142
e-value: 5.5E-33
score: 115.3
IPR036444Phospholipase A2 domain superfamilySUPERFAMILYSSF48619Phospholipase A2, PLA2coord: 33..137
NoneNo IPR availablePANTHERPTHR11716:SF47PHOSPHOLIPASE A2-BETA-RELATEDcoord: 13..140
IPR001211Phospholipase A2PANTHERPTHR11716PHOSPHOLIPASE A2coord: 13..140
IPR033113Phospholipase A2, histidine active sitePROSITEPS00118PA2_HIScoord: 75..82

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg10813Csa2G000560Cucumber (Chinese Long) v2carcuB0598
Carg10813MELO3C004617Melon (DHL92) v3.5.1carmeB0577
Carg10813CSPI02G00550Wild cucumber (PI 183967)carcpiB0603
Carg10813Cucsa.116920Cucumber (Gy14) v1carcgyB0644
Carg10813Lsi11G001980Bottle gourd (USVL1VR-Ls)carlsiB514
Carg10813Cp4.1LG11g11590Cucurbita pepo (Zucchini)carcpeB0809
Carg10813CsaV3_2G001580Cucumber (Chinese Long) v3carcucB0605
Carg10813Cla97C11G208250Watermelon (97103) v2carwmbB0591
Carg10813Bhi06G001427Wax gourdcarwgoB0675
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg10813Silver-seed gourdcarcarB044
Carg10813Silver-seed gourdcarcarB104
Carg10813Silver-seed gourdcarcarB140
Carg10813Silver-seed gourdcarcarB233
Carg10813Silver-seed gourdcarcarB527
Carg10813Silver-seed gourdcarcarB528
Carg10813Cucumber (Gy14) v2carcgybB0572
Carg10813Cucumber (Gy14) v2carcgybB0573
Carg10813Cucumber (Gy14) v1carcgyB0646
Carg10813Cucurbita maxima (Rimu)carcmaB0793
Carg10813Cucurbita maxima (Rimu)carcmaB0795
Carg10813Cucurbita maxima (Rimu)carcmaB0797
Carg10813Cucurbita maxima (Rimu)carcmaB0798
Carg10813Cucurbita maxima (Rimu)carcmaB0802
Carg10813Cucurbita moschata (Rifu)carcmoB0772
Carg10813Cucurbita moschata (Rifu)carcmoB0774
Carg10813Cucurbita moschata (Rifu)carcmoB0775
Carg10813Cucurbita moschata (Rifu)carcmoB0776
Carg10813Cucurbita moschata (Rifu)carcmoB0778
Carg10813Cucurbita moschata (Rifu)carcmoB0779
Carg10813Cucurbita pepo (Zucchini)carcpeB0812
Carg10813Cucurbita pepo (Zucchini)carcpeB0813
Carg10813Cucurbita pepo (Zucchini)carcpeB0814
Carg10813Cucurbita pepo (Zucchini)carcpeB0817
Carg10813Wild cucumber (PI 183967)carcpiB0602
Carg10813Cucumber (Chinese Long) v2carcuB0597
Carg10813Cucumber (Chinese Long) v3carcucB0604
Carg10813Bottle gourd (USVL1VR-Ls)carlsiB519
Carg10813Melon (DHL92) v3.5.1carmeB0573
Carg10813Melon (DHL92) v3.6.1carmedB0580
Carg10813Melon (DHL92) v3.6.1carmedB0584
Carg10813Watermelon (Charleston Gray)carwcgB0555
Carg10813Watermelon (Charleston Gray)carwcgB0553
Carg10813Watermelon (97103) v1carwmB0565
Carg10813Watermelon (97103) v1carwmB0569
Carg10813Watermelon (97103) v2carwmbB0593
Carg10813Wax gourdcarwgoB0684