Carg08994 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg08994
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	beta-carotene isomerase D27, chloroplastic-like
Location	Cucurbita_argyrosperma_scaffold_020 : 1114677 .. 1115795 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGTAAAATTAATTCAAGAAAGGAGGGTCTTTTTGGGGAATTGGGGAAACTTGAGGGGCAACCACAAAAGAAAGTTGAGAAGGAAAACCAATTGTTGTGTTGCAGTGCTCACCGGAGCGGCAGAGAGAGGAGGAAATGAAGGAACGAGAAATCTCAATACGGTTTATACAGATAATTGGTTTGATAAGTTGGCTATTGACCATCTATCTGAGGCAGTACAGGCAACTTCAGGTGATTCATTTTAGTTTCCTTTTAATAAAATGGTAAAATGGAGTTATGGGGTAGGCCATATGTTTGCAGGTTGGAGAAGCAAAAAAAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAATTTGAACCAAATCAAACAAAGAGAGGTCATGATTGAGGCACTTCAAAGGGCGTTTCCAAGGCCAATACTCTCTTTGGTAAACCCTCCTTCATTACCATATTTAATACCCTTTTTGGATCACGTCTAGTTTTTTCATTCTACTCAAACTGCAGATAAGAACATTGCTGCCACAATCCAAATTCGCCAGAGAATATTTTGCCGCATTCACCACTGTCTTCTTTGCTTGGTTAGTCGGACCCTGCGAGGTAAATTTACCCGAAGCCCCTTTCACTCCGCATGGGATTCAAGAACATTTTTTTTTAAAAAAAAATTACTTGATACGCAGGTGAAGGAATCAGAGTTGAATGGAAGAAGAGAGAGGAATGTTGTTCATATCCACAAGTGCAGGTATCTTTGTCTCGTGCCAGAGTTGTATAATAATAATAATAATTATTATTATTATGCTTCCTCTAAGCTAAGCCCTCCGTACTTGCAGGTTCTTAGAGCAGACAAACTGTGTTGGAATGTGCATTAATCTATGCAAGTTTCCAACTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTGAGCATGATCCCCAGTAAGTCTCCTCACTAAGCTACACAATCACCAAACATTTGCAGCAACCAAAGCAGCGTGTCTTCTTCTTCTTTGCAGATTTTGATGACATGAGCTGTGAGATGAAATTTGGAGAGGACCCACCAGCTTCAATCGAAGATCCAGCGCTCAACCAGCCATGTTACACACTA

mRNA sequence

ATGGATGTAAAATTAATTCAAGAAAGGAGGGTCTTTTTGGGGAATTGGGGAAACTTGAGGGGCAACCACAAAAGAAAGTTGAGAAGGAAAACCAATTGTTGTGTTGCAGTGCTCACCGGAGCGGCAGAGAGAGGAGGAAATGAAGGAACGAGAAATCTCAATACGGTTTATACAGATAATTGGTTTGATAAGTTGGCTATTGACCATCTATCTGAGGCAGTACAGGCAACTTCAGGTTGGAGAAGCAAAAAAAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAATTTGAACCAAATCAAACAAAGAGAGGTCATGATTGAGGCACTTCAAAGGGCGTTTCCAAGGCCAATACTCTCTTTGATAAGAACATTGCTGCCACAATCCAAATTCGCCAGAGAATATTTTGCCGCATTCACCACTGTCTTCTTTGCTTGGTTAGTCGGACCCTGCGAGGTGAAGGAATCAGAGTTGAATGGAAGAAGAGAGAGGAATGTTGTTCATATCCACAAGTGCAGGTTCTTAGAGCAGACAAACTGTGTTGGAATGTGCATTAATCTATGCAAGTTTCCAACTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTGAGCATGATCCCCAATTTTGATGACATGAGCTGTGAGATGAAATTTGGAGAGGACCCACCAGCTTCAATCGAAGATCCAGCGCTCAACCAGCCATGTTACACACTA

Coding sequence (CDS)

ATGGATGTAAAATTAATTCAAGAAAGGAGGGTCTTTTTGGGGAATTGGGGAAACTTGAGGGGCAACCACAAAAGAAAGTTGAGAAGGAAAACCAATTGTTGTGTTGCAGTGCTCACCGGAGCGGCAGAGAGAGGAGGAAATGAAGGAACGAGAAATCTCAATACGGTTTATACAGATAATTGGTTTGATAAGTTGGCTATTGACCATCTATCTGAGGCAGTACAGGCAACTTCAGGTTGGAGAAGCAAAAAAAGTGGATATGAAAGCTTGGTGGAAGTGGCAACAATGGCGTCAAGAAATTTGAACCAAATCAAACAAAGAGAGGTCATGATTGAGGCACTTCAAAGGGCGTTTCCAAGGCCAATACTCTCTTTGATAAGAACATTGCTGCCACAATCCAAATTCGCCAGAGAATATTTTGCCGCATTCACCACTGTCTTCTTTGCTTGGTTAGTCGGACCCTGCGAGGTGAAGGAATCAGAGTTGAATGGAAGAAGAGAGAGGAATGTTGTTCATATCCACAAGTGCAGGTTCTTAGAGCAGACAAACTGTGTTGGAATGTGCATTAATCTATGCAAGTTTCCAACTCAGGATTTCATCAAGGATTCTTTGGGAATGCCAGTGAGCATGATCCCCAATTTTGATGACATGAGCTGTGAGATGAAATTTGGAGAGGACCCACCAGCTTCAATCGAAGATCCAGCGCTCAACCAGCCATGTTACACACTA

Protein sequence

MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDNWFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPCYTL

Homology

[Close]

BLAST of Carg08994 vs. NCBI nr
Match: XP_022931142.1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 497.3 bits (1279), Expect = 3.0e-137
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0


Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRVFLGNWGNLRGNHKRKLRRKT CCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTTCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 243

[Close]

BLAST of Carg08994 vs. NCBI nr
Match: XP_023534254.1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 492.7 bits (1267), Expect = 7.4e-136
Identity = 237/243 (97.53%), Postives = 238/243 (97.94%), Query Frame = 0


Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRV LGNWGN+RGNHKRKLRRK NCCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVILGNWGNMRGNHKRKLRRKANCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIR LLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 243

[Close]

BLAST of Carg08994 vs. NCBI nr
Match: XP_022931141.1 (beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 491.1 bits (1263), Expect = 2.1e-135
Identity = 240/248 (96.77%), Postives = 240/248 (96.77%), Query Frame = 0


Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRVFLGNWGNLRGNHKRKLRRKT CCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTTCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATS-----GWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQ 120
           WFDKLAIDHLSEAVQATS     GWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQ
Sbjct: 61  WFDKLAIDHLSEAVQATSGHMFAGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQ 120

Query: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180
           RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK
Sbjct: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180

Query: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240
           CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA
Sbjct: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240

Query: 241 LNQPCYTL 244
           LNQPCY L
Sbjct: 241 LNQPCYKL 248

[Close]

BLAST of Carg08994 vs. NCBI nr
Match: XP_023534253.1 (beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.5 bits (1251), Expect = 5.3e-134
Identity = 237/248 (95.56%), Postives = 238/248 (95.97%), Query Frame = 0


Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRV LGNWGN+RGNHKRKLRRK NCCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVILGNWGNMRGNHKRKLRRKANCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATS-----GWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQ 120
           WFDKLAIDHLSEAVQATS     GWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQ
Sbjct: 61  WFDKLAIDHLSEAVQATSGHIFAGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQ 120

Query: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180
           RAFPRPILSLIR LLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK
Sbjct: 121 RAFPRPILSLIRALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180

Query: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240
           CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA
Sbjct: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240

Query: 241 LNQPCYTL 244
           LNQPCY L
Sbjct: 241 LNQPCYKL 248

[Close]

BLAST of Carg08994 vs. NCBI nr
Match: XP_022995501.1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 481.1 bits (1237), Expect = 2.2e-132
Identity = 232/243 (95.47%), Postives = 234/243 (96.30%), Query Frame = 0


Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRV LGNWGNLRGNHKRKLRR  NCCVAVLTGAAERGGNE TRNLN VY DN
Sbjct: 1   MDVKLIQERRVILGNWGNLRGNHKRKLRRNANCCVAVLTGAAERGGNEETRNLNAVYRDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRS+KSGYESLVEVATMASRNLNQIKQRE MIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSEKSGYESLVEVATMASRNLNQIKQREAMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQ+FIKDSLGMPVSM PNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQEFIKDSLGMPVSMTPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 243

[Close]

BLAST of Carg08994 vs. TAIR10
Match: AT1G03055.1 (unknown protein)

HSP 1 Score: 216.5 bits (550), Expect = 1.9e-56
Identity = 115/251 (45.82%), Postives = 163/251 (64.94%), Query Frame = 0


Query: 1   MDVKL-IQERRVF-LGNWGN--LRGNHKRKLRRKTNCCVAVLTG----AAERGGNEGTRN 60
           M+ KL + + ++F    W N    G  +R     T C   V +G    A E    E +  
Sbjct: 1   MNTKLSLSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNT 60

Query: 61  LNTVYTDNWFDKLAIDHLSEAVQATSG--WRSKKSGYESLVEVATMASRNLNQIKQREVM 120
            N    D++F K+AI++LS+ +Q  +G    SK + Y+ LV+ AT  SRN +  +Q E +
Sbjct: 61  KNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFV 120

Query: 121 IEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNV 180
           + +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP EV+E+E+NGR+E++V
Sbjct: 121 LSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSV 180

Query: 181 VHIHKCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPAS 240
           V+I KCRFLEQ+NCVGMC ++CK P+Q FIK+SLGMP+ M P+F+D+SC+M FG +PP  
Sbjct: 181 VYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEI 240

Query: 241 IEDPALNQPCY 242
            +DPA+ QPC+
Sbjct: 241 EDDPAMKQPCF 251

[Close]

BLAST of Carg08994 vs. TAIR10
Match: AT1G64680.1 (unknown protein)

HSP 1 Score: 166.0 bits (419), Expect = 2.9e-41
Identity = 87/221 (39.37%), Postives = 123/221 (55.66%), Query Frame = 0


Query: 28  RRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDNWFDKLAIDHLSEAVQATSGWRSKKS-- 87
           RR T C      G AE  G      L T Y D   +++ +   +  +      + K +  
Sbjct: 16  RRSTRC------GIAEPSGEPAPMGLKTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKE 75

Query: 88  ------GYESLVEVATMASRNLNQIKQREVMIEALQRAFPRPILSLIRTLLPQSKFAREY 147
                  YES VEV+    +  ++++Q+E + E L    P       R L P +K+A E+
Sbjct: 76  KGFWEYDYESFVEVSKRVMQGRSRVQQQEAVREVLLSMLPPGAPEQFRKLFPPTKWAAEF 135

Query: 148 FAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDF 207
            AA T  FF WLVGP +V E E+NG ++R+ V I KCR+LE + CVGMC+N+CK PTQDF
Sbjct: 136 NAALTVPFFHWLVGPSQVIEVEVNGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDF 195

Query: 208 IKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 241
             +  G+P++M PN++DMSCEM +G+ PPA  ED A  QPC
Sbjct: 196 FTNEFGLPLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPC 230

[Close]

BLAST of Carg08994 vs. TAIR10
Match: AT4G01995.1 (unknown protein)

HSP 1 Score: 119.4 bits (298), Expect = 3.1e-27
Identity = 74/220 (33.64%), Postives = 112/220 (50.91%), Query Frame = 0


Query: 27  LRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDNWFDKLAIDHLSEAVQATSGWRSKKSG 86
           L R+  C +   + ++E   +EG   L   Y     D   +      +    G  S+K G
Sbjct: 27  LTRRLRCRI---SNSSEVKSDEGAPKLE--YKPGPLDDFFMQSFRNKLVEEVGSDSEKPG 86

Query: 87  YESLVE-VATMASRNLNQIKQREVMIEALQRAFPRPILSLIRTL---LPQSKFAREYFAA 146
           Y  L+E V  +  +   + +  +  +  L+  FP  IL L + L   + Q K A    A 
Sbjct: 87  YVGLIELVKLLLLKGRTRSETSDAAVRILKSLFPPLILELYKLLIAPIAQGKLAALMVAR 146

Query: 147 FTTVFFAWLVGPCEVKESEL-NGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDFIK 206
            T +   WL+GP +V   +L NG    + V + KC++LE++ CVG+CIN CK PTQ F K
Sbjct: 147 VTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVEKCQYLEESKCVGVCINTCKLPTQTFFK 206

Query: 207 DSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPCY 242
           D +G+P+ M PNF D SC+ KFG  PP   +D  +N+PC+
Sbjct: 207 DYMGVPLVMEPNFKDYSCQFKFGVAPPE--DDGNVNEPCF 239

[Close]

BLAST of Carg08994 vs. Swiss-Prot
Match: sp|C7AU21|D27_ORYSJ (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 7.9e-65
Identity = 116/187 (62.03%), Postives = 146/187 (78.07%), Query Frame = 0


Query: 55  TVYTDNWFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEAL 114
           TVY D+WFDKLAI +LS  +Q  SG +++K GYESL++ A   SR  +  KQ E++ +AL
Sbjct: 78  TVYRDSWFDKLAIGYLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQSEIVTQAL 137

Query: 115 QRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIH 174
           +RA P  IL++I+ ++P S+F+REYFAAFTT+FF WLVGPCEV ESE+ GR+E+NVV+I 
Sbjct: 138 ERALPSYILTMIKVMMPPSRFSREYFAAFTTIFFPWLVGPCEVMESEVEGRKEKNVVYIP 197

Query: 175 KCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDP 234
           KCRFLE TNCVGMC NLCK P Q FI+DSLGM V M PNF+DMSCEM FG+ PP   +DP
Sbjct: 198 KCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCEMIFGQQPPE--DDP 257

Query: 235 ALNQPCY 242
           AL QPC+
Sbjct: 258 ALKQPCF 262

[Close]

BLAST of Carg08994 vs. Swiss-Prot
Match: sp|Q7XA78|D27_ARATH (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.3e-55
Identity = 115/251 (45.82%), Postives = 163/251 (64.94%), Query Frame = 0


Query: 1   MDVKL-IQERRVF-LGNWGN--LRGNHKRKLRRKTNCCVAVLTG----AAERGGNEGTRN 60
           M+ KL + + ++F    W N    G  +R     T C   V +G    A E    E +  
Sbjct: 1   MNTKLSLSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNT 60

Query: 61  LNTVYTDNWFDKLAIDHLSEAVQATSG--WRSKKSGYESLVEVATMASRNLNQIKQREVM 120
            N    D++F K+AI++LS+ +Q  +G    SK + Y+ LV+ AT  SRN +  +Q E +
Sbjct: 61  KNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFV 120

Query: 121 IEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNV 180
           + +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP EV+E+E+NGR+E++V
Sbjct: 121 LSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSV 180

Query: 181 VHIHKCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPAS 240
           V+I KCRFLEQ+NCVGMC ++CK P+Q FIK+SLGMP+ M P+F+D+SC+M FG +PP  
Sbjct: 181 VYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEI 240

Query: 241 IEDPALNQPCY 242
            +DPA+ QPC+
Sbjct: 241 EDDPAMKQPCF 251

[Close]

BLAST of Carg08994 vs. TrEMBL
Match: tr|A0A0A0LIR1|A0A0A0LIR1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G226860 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.8e-101
Identity = 185/243 (76.13%), Postives = 205/243 (84.36%), Query Frame = 0


Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MD+++IQ+ RV L    N   N+K KLRRK+  C  VLT  AE    E TR  NTVYTDN
Sbjct: 1   MDLRVIQDTRVILPTMMN---NNKMKLRRKSILCFGVLTRPAEGELIEETRKTNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDK+AIDHLS+AVQATSGWRSKKSGYESLVEV TMASRN N IKQ+EV+I+AL  AFP+
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLVEVTTMASRNFNHIKQKEVVIQALGMAFPK 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLI+ LLPQSK AREYFAAFTTVFFAWLVGPCEVKESE  G+RE+NVV IHKCRFLE
Sbjct: 121 PILSLIKALLPQSKLAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNC GMCINLCKFP QDFIKDSLGMPV+M+PNFDDMSCEM FG++PPASI+DPAL QPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 240

[Close]

BLAST of Carg08994 vs. TrEMBL
Match: tr|A0A1S3BL84|A0A1S3BL84_CUCME (LOW QUALITY PROTEIN: beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491262 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.0e-101
Identity = 185/243 (76.13%), Postives = 206/243 (84.77%), Query Frame = 0


Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MD+++IQ+  V L    N   N+K KLRRKT   V VLT +AE    E TR   TVYTDN
Sbjct: 1   MDLRVIQDTTVILPTMMN---NNKMKLRRKTFFXVGVLTRSAEGEVIEETRKTKTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDK+AIDHLS+AVQATSGWRSKKSGYESL+EV TMASRN N IKQ+EV+I+AL RAFP+
Sbjct: 61  WFDKIAIDHLSQAVQATSGWRSKKSGYESLLEVTTMASRNFNHIKQKEVVIQALGRAFPK 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLI+ LLPQSKFAREYFAAFTTVFFAWLVGPCEVKESE  G+RE+NVV IHKCRFLE
Sbjct: 121 PILSLIKALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESEFKGKREKNVVQIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNC GMCINLCKFP QDFIKDSLGMPV+M+PNFDDMSCEM FG++PPASI+DPAL QPC
Sbjct: 181 QTNCAGMCINLCKFPCQDFIKDSLGMPVTMVPNFDDMSCEMIFGKEPPASIDDPALKQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 240

[Close]

BLAST of Carg08994 vs. TrEMBL
Match: tr|A0A2I4GEB7|A0A2I4GEB7_9ROSI (beta-carotene isomerase D27, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC109007147 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.3e-83
Identity = 154/207 (74.40%), Postives = 176/207 (85.02%), Query Frame = 0


Query: 35  VAVLTGAAERGGNEGTRNLNTVYTDNWFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVA 94
           +AVLT  +E    E  +     Y DNWFD++AI+HLS++VQA +G R+ KSGYESLVE A
Sbjct: 32  LAVLTRPSENVTKEARK--TNFYNDNWFDQIAINHLSQSVQAATGLRNSKSGYESLVEAA 91

Query: 95  TMASRNLNQIKQREVMIEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGP 154
            MAS+N N I+QREV+I AL+RAFPRPILSLI+TLLPQSK AREYFAAFTTVFFAWLVG 
Sbjct: 92  RMASQNFNPIQQREVVIHALERAFPRPILSLIKTLLPQSKLAREYFAAFTTVFFAWLVGY 151

Query: 155 CEVKESELNGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNF 214
           CEV+ESELNGRRERNVVHI KCRFLE+TNCVGMCINLCK P+Q FIKDSLGMPV+M+PNF
Sbjct: 152 CEVRESELNGRRERNVVHIKKCRFLEETNCVGMCINLCKMPSQAFIKDSLGMPVNMVPNF 211

Query: 215 DDMSCEMKFGEDPPASIEDPALNQPCY 242
           DDMSCEM FG+D PAS +DPAL QPCY
Sbjct: 212 DDMSCEMIFGQDSPASTDDPALKQPCY 236

[Close]

BLAST of Carg08994 vs. TrEMBL
Match: tr|A0A2I4G2B0|A0A2I4G2B0_9ROSI (beta-carotene isomerase D27, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC109004072 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.2e-81
Identity = 151/212 (71.23%), Postives = 179/212 (84.43%), Query Frame = 0


Query: 35  VAVLT-----GAAERGGNEGTRNLNTVYTDNWFDKLAIDHLSEAVQATSGWRSKKSGYES 94
           VAVLT       AE    + T + ++VY DNWFD +AI+HLS++VQA +G R+ KSGYES
Sbjct: 37  VAVLTTRPKESIAEDLSGKSTSS-SSVYNDNWFDLVAINHLSQSVQAATGIRNSKSGYES 96

Query: 95  LVEVATMASRNLNQIKQREVMIEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFA 154
           LVE A +AS+N N I+Q+EV+I+AL+RAFPRPILSLI+ LLPQSKFAREYFAAFTTVFFA
Sbjct: 97  LVEAAKVASQNFNPIQQKEVIIQALERAFPRPILSLIKILLPQSKFAREYFAAFTTVFFA 156

Query: 155 WLVGPCEVKESELNGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVS 214
           WLVGPCEV+E ELNGRRE+NVVHI KCRFLE+T CVG+C+NLCK P+Q FIKDS G+PV+
Sbjct: 157 WLVGPCEVRELELNGRREKNVVHIKKCRFLEETGCVGLCVNLCKMPSQTFIKDSFGIPVN 216

Query: 215 MIPNFDDMSCEMKFGEDPPASIEDPALNQPCY 242
           M+PNFDDMSCEM FG+DPPAS EDPAL QPCY
Sbjct: 217 MVPNFDDMSCEMIFGQDPPASTEDPALKQPCY 247

[Close]

BLAST of Carg08994 vs. TrEMBL
Match: tr|A0A2P5FB30|A0A2P5FB30_9ROSA (Beta-carotene isomerase D27-like OS=Trema orientalis OX=63057 GN=TorD27 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 3.4e-81
Identity = 148/222 (66.67%), Postives = 180/222 (81.08%), Query Frame = 0


Query: 29  RKTNCCVAVLTGAAER------GGNEGTRNLNTV-YTDNWFDKLAIDHLSEAVQATSGWR 88
           ++++  VAVLT  A+       G +   R ++   Y D WFD++AI HLS++VQA +G R
Sbjct: 30  KRSSIAVAVLTPPADNISMVTDGSSLRPREVSPAPYNDTWFDRIAIQHLSQSVQAATGLR 89

Query: 89  SKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPRPILSLIRTLLPQSKFAREYFA 148
           + KSGYESLVE A  ASRN    KQRE++I+AL++AFP+PILSLIRTL+PQSKFAREYFA
Sbjct: 90  NSKSGYESLVEAAAAASRNFGPTKQRELVIQALEKAFPKPILSLIRTLMPQSKFAREYFA 149

Query: 149 AFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDFIK 208
            FTT+FFAWLVGPCEV+ESELNGRRERNVVHI+KCRFLEQTNC GMC NLCK P+Q FIK
Sbjct: 150 VFTTLFFAWLVGPCEVRESELNGRRERNVVHINKCRFLEQTNCAGMCSNLCKVPSQTFIK 209

Query: 209 DSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPCYTL 244
           DSLGM V+M+PNF+DMSCEMKFG+DPP+ ++DPA  QPCY L
Sbjct: 210 DSLGMSVNMVPNFEDMSCEMKFGQDPPSLMDDPAFKQPCYKL 251

The following BLAST results are available for this feature:

BLAST of Carg08994 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022931142.1	3.0e-137	98.77	beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita moschata]	[more]
XP_023534254.1	7.4e-136	97.53	beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita pepo subsp...	[more]
XP_022931141.1	2.1e-135	96.77	beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
XP_023534253.1	5.3e-134	95.56	beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita pepo subsp...	[more]
XP_022995501.1	2.2e-132	95.47	beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg08994 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G03055.1	1.9e-56	45.82	unknown protein	[more]
AT1G64680.1	2.9e-41	39.37	unknown protein	[more]
AT4G01995.1	3.1e-27	33.64	unknown protein	[more]

BLAST of Carg08994 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|C7AU21\|D27_ORYSJ	7.9e-65	62.03	Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39...	[more]
sp\|Q7XA78\|D27_ARATH	3.3e-55	45.82	Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D2...	[more]

BLAST of Carg08994 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LIR1\|A0A0A0LIR1_CUCSA	1.8e-101	76.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G226860 PE=4 SV=1	[more]
tr\|A0A1S3BL84\|A0A1S3BL84_CUCME	3.0e-101	76.13	LOW QUALITY PROTEIN: beta-carotene isomerase D27, chloroplastic OS=Cucumis melo ...	[more]
tr\|A0A2I4GEB7\|A0A2I4GEB7_9ROSI	1.3e-83	74.40	beta-carotene isomerase D27, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC...	[more]
tr\|A0A2I4G2B0\|A0A2I4G2B0_9ROSI	1.2e-81	71.23	beta-carotene isomerase D27, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC...	[more]
tr\|A0A2P5FB30\|A0A2P5FB30_9ROSA	3.4e-81	66.67	Beta-carotene isomerase D27-like OS=Trema orientalis OX=63057 GN=TorD27 PE=4 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR038938	D27-like
IPR025114	DUF4033

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08994-RA	Carg08994-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025114	Domain of unknown function DUF4033	PFAM	PF13225	DUF4033	coord: 150..229 e-value: 2.1E-34 score: 117.3
IPR038938	Beta-carotene isomerase D27-like	PANTHER	PTHR33591	FAMILY NOT NAMED	coord: 24..243
None	No IPR available	PANTHER	PTHR33591:SF3	BETA-CAROTENE ISOMERASE D27, CHLOROPLASTIC	coord: 24..243

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg08994	Cla006026	Watermelon (97103) v1	carwmB0778
Carg08994	Csa2G226860	Cucumber (Chinese Long) v2	carcuB0792
Carg08994	MELO3C014318	Melon (DHL92) v3.5.1	carmeB0776
Carg08994	ClCG07G000550	Watermelon (Charleston Gray)	carwcgB0760
Carg08994	CSPI02G11300	Wild cucumber (PI 183967)	carcpiB0811
Carg08994	Cucsa.016410	Cucumber (Gy14) v1	carcgyB0886
Carg08994	CmaCh02G017790	Cucurbita maxima (Rimu)	carcmaB1084
Carg08994	CmaCh19G000140	Cucurbita maxima (Rimu)	carcmaB1080
Carg08994	CmoCh02G018210	Cucurbita moschata (Rifu)	carcmoB1057
Carg08994	CmoCh19G000130	Cucurbita moschata (Rifu)	carcmoB1053
Carg08994	Lsi05G009680	Bottle gourd (USVL1VR-Ls)	carlsiB717
Carg08994	Cp4.1LG15g00130	Cucurbita pepo (Zucchini)	carcpeB1092
Carg08994	Cp4.1LG05g01570	Cucurbita pepo (Zucchini)	carcpeB1099
Carg08994	CsGy2G011330	Cucumber (Gy14) v2	carcgybB0769
Carg08994	MELO3C014318.2	Melon (DHL92) v3.6.1	carmedB0789
Carg08994	CsaV3_2G013780	Cucumber (Chinese Long) v3	carcucB0813
Carg08994	Cla97C07G128850	Watermelon (97103) v2	carwmbB0821
Carg08994	Bhi05G000205	Wax gourd	carwgoB0916

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg08994	Carg16820	Silver-seed gourd	carcarB598

The following block(s) are covering this gene:


Gene	Organism	Block
Carg08994	Silver-seed gourd	carcarB264
Carg08994	Silver-seed gourd	carcarB273
Carg08994	Silver-seed gourd	carcarB599
Carg08994	Silver-seed gourd	carcarB610
Carg08994	Cucumber (Gy14) v2	carcgybB0772
Carg08994	Cucumber (Gy14) v1	carcgyB0888
Carg08994	Cucurbita maxima (Rimu)	carcmaB1075
Carg08994	Cucurbita maxima (Rimu)	carcmaB1089
Carg08994	Cucurbita moschata (Rifu)	carcmoB1049
Carg08994	Cucurbita moschata (Rifu)	carcmoB1052
Carg08994	Cucurbita moschata (Rifu)	carcmoB1058
Carg08994	Cucurbita pepo (Zucchini)	carcpeB1087
Carg08994	Cucurbita pepo (Zucchini)	carcpeB1096
Carg08994	Cucurbita pepo (Zucchini)	carcpeB1098
Carg08994	Cucurbita pepo (Zucchini)	carcpeB1101
Carg08994	Wild cucumber (PI 183967)	carcpiB0815
Carg08994	Cucumber (Chinese Long) v2	carcuB0795
Carg08994	Cucumber (Chinese Long) v3	carcucB0818
Carg08994	Bottle gourd (USVL1VR-Ls)	carlsiB720
Carg08994	Melon (DHL92) v3.5.1	carmeB0775
Carg08994	Melon (DHL92) v3.5.1	carmeB0781
Carg08994	Melon (DHL92) v3.6.1	carmedB0788
Carg08994	Melon (DHL92) v3.6.1	carmedB0792
Carg08994	Watermelon (Charleston Gray)	carwcgB0758
Carg08994	Watermelon (97103) v1	carwmB0782
Carg08994	Watermelon (97103) v2	carwmbB0817
Carg08994	Watermelon (97103) v2	carwmbB0819
Carg08994	Wax gourd	carwgoB0914