Carg05777 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg05777
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	MIP18 family protein
Location	Cucurbita_argyrosperma_scaffold_033 : 980732 .. 984243 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGCTGGGGCTTATTAATGCGAACCCGGTGGTTCACGCCAAGAAGGAGCGGATCGCTCGCTCCGAGGACTTTCACGGCGACGACGCTGTTGATCCTCTCGAAATCTATGATATCCTTTTCTTAATTGATCGTGTTTTGACCGAATTTTGAGTTCTCCTTTCGTTTTCTCTTCAGGTCTATTTTCACTGTGATTCTCGAGCTCAATTCTGATTAGGAAGTTATTAGGTGTTGTTTTAGTCTATTTTTAGAGGATTCTTTTTTTGTTTTTTGTTTTTTAATTTTCTGGCGCTTTCTTTGTGGCCGAACGAGTGATTTTTTGGCTGATTTTGTGAAAATTCAGTTTTCTCGTTTCTTTTGGAATCTGTTATTTGATAGTCAATTTGCAAATTTCTTGTCCTTGACTCGTATTTTGGCACATTTCGTGAGGGATATTAGAGATCCGGAGCATCCGTATTCGTTAGAGCAGCTTAGTGTTCTTTCAGAGGAATCGATCACCGTTGATGAGAAGCTAGGGCGTATCCTGTAAGCACAACCGAAATAGCTCCATTACTTCTCCGCCTCTTTGATTTTGAGAGGCAATTTGAGATTATTGATTTCTAGCCATATTGATTTAACAAACTCATTGCCAAAGACTCCTAGTCTAGGGAAGTTTGATTGAAATGCCTTCAGCTTCAAATTCCTAAATCGTTTGCTGAATTAATGTTTATCTCACAGGATTACTTTCACTCCGACTATCCAGCATTGTAGTATGGCGACTGTGATTGGCCTCTGTCTGAGAGTGAAACTTAAACACTTCTTCCCCCCACATTACAAGGTCAGATCATTTTCCTTTCTTAACAATAGGCTTGAACTTTGGATTACTGCTTCTTCATTTTCAGTTCTACACTCTACTTGATGCAATCACCAGATACTGATGAAATTTGTGTTTTATGTATGGATATGAATTCAAGTTTGAAGAAGTTTGTTGGCTTTTGAAATAACTGATCATGATGATAGTGCGCTTGTGTTCCATCTCACTTGCCAGTACCGTTTGGATGCAACTTTTAGATTTGTTCTTTTGGTGCCTGTTTGATAAACTTGATGGATTATGCATATTAGGTAGCCGTTTAACGAAACTGTAGAAGAACTTATTATTCAAGAAACTAAATTATTGATGAAAGCACATAAAGAGCAGAATTAAAACAAAAAAGAAAATAAAAATTACTTACGCTTGAGTTTTTTTTTTTTTTTTTTTTAATAAACAATTTTGAAAACAATTTAAATTTTTTAAAAAAATCCCACAGTCCTGTTTTAATTTTTGTATTTATTGTTTTTACTTTTAAAAATTATTTGGAAAGGTATCTCGATCTTGCTTTTCATGTAAGTTATACCTAAAATATTTAATTATTTTTTATTAACAATAATTTTGACAATATAAGAAAAACGAAAAATAGAAAAGGTTGTTTTCTCTTTTATTTTAAATTTATCATCTCAATTTCAAAAAAACACACGTATACACATTAAAAAGACAACCTCTGTAGTTTAGTTTGTGCAGAGAACTAGAATTTATAGGACTTATTTGTGAATTTTGTTGTAATAACAAGAGACAATCATCTATTCAATTCAAATGAATCCCTGTACAAGACTTTTGTTCAATGTTCATTTAATTTCATGTTTGAATATATATGATGGCAAAAGGCTAATTACTTTATTATCTTATTAGCCGATCTGATATAATCATGAAATCAACTTGAACAATTTGAATCTAGCTCGAGTTTAGATCGTCCACTATGTTTGCTTATTATAGAATTTGCAGGTTGACATCAAAGTGTCTCCAGGATCCCACGCAAATGAAGAGTCAGGTGAGATTAGAACATTCTAAGGTGTTATTAGATCTAGATACCCATTGGAGAAAAGATAATGAAATCAAAGATTGAACTTGTTAGAACCTTAAATATTTGATAACGAATATTTCTAATGAAGGTATAAATATTCGAGATTGGTTGAATCACCATTAATTTGGTTCCATTCTATTATAATCAAATAATTGTGCATAACGGAACTGAATTCACTCTATTTGAGTTAGTTCAGTTTGGTAAATTAGGTTCGTTCAGTTTGGTAAATTACCGCTGACAAACTCGTGCATTGTGTTTTTTGATCTTTAAATTTGTTCTGTTTTGTTCTTTGTTTTTGGCGGTGAAAGAACAGTCAATAAGCAGCTAAATGACAAAGAAAGAGTTGCAGCTGCTTTGGAGAATCCAAATCTGCGTCAGCTTGTAGATGAATGCCTGGATTCCAGTGAGTTCTGAATAAATCAAATATCATCGTACGGATGGATAAGAAAAAAGGTAGTTCCCTAACGTTACAAAATAGAGGTTAAAGTATATAAACTACCCCTTAAAATCTCAGACTTAAAGTTCTACCCTTACTTTTCAATTTCAAAATTGTTCAGTAAATATAAAAATATAGAAACATAGATACATATAGATACATAGATCTATATAGCATCTAGATATATAGATCTCTATACATTTATCATATAGATCTCTATATCGATATATATCTATGATATAGATATCTATATCGATATATATCTATGATATAGATATCTATATAATATATAGAGATATCTATAGATCTATATATAAATATCCATATATAAAAAATGATTTCAGTTATTTCAATTATGCTCCTAATTTTTTTTTTATATCTATTATATAGGTATCTATATATCTATATATAAATACATATATAAAAAAATGATTTCAGTTATTTCAATTATGCTCCTAATTTTTTTTTTTATATCTATTATATAGGTATCTATATATCTATATATAAATATCCATATATAAAAAATGATTTCAGTTATAAATATATCCATCTATATCCATATATATATGGATATATCCATTTTTATATCTATTATATAGATATCATTTTTTTTTTTTATTCTCCGTGTAATGAAATTCTTAATTGGCTGAAAAGAAAAATGAAGTTCAACACATTATTGGGTACTTCAATTTTGATTCAAACCAACCAGCTTTACATGAAATGGGGAAGTGGATGTGTTTAGATTCTTCTAAATTTATTTGCTATTTCTCCGTCTATTATGAGAATAGTGTTATTTCTGGTTGCCGGGTTATTCTTTTGAGTCCGAATATTCAATACTTAATACTATAAAAAATAAAAAAGAATTTGTTTCTAATTTTTAAAAATTGGAGATTTCTTTTTATGTGCACATCACTTGATAACAATTTTGAATTTGCAGGTTGAGTTTGTATAACTTAAAGTGGGTGATGGAAATCTGGCTTGCTATGTGAGTTTATTTATTTCTGGTTTAACCAACTTCAATTTATTTAATAATTTTAATCTTTTTAAAAGACTTTTGAGTGAGTGATATGTTTGAGGATATTATTCTAAGCTAAGGGGATGATATTTTTAAGTAGAAATAGGAAAATAATGAGTTTTGGTATATGACTGTGTTATCAACTCTTTTGAGCAATTGATATTCATACCATAACCTACATGAT

mRNA sequence

ATGACGCTGGGGCTTATTAATGCGAACCCGGTGGTTCACGCCAAGAAGGAGCGGATCGCTCGCTCCGAGGACTTTCACGGCGACGACGCTGTTGATCCTCTCGAAATCTATGATATCCTTTTCTTAATTGATCGTTTTTCTCGTTTCTTTTGGAATCTGTTATTTGATAGTCAATTTGCAAATTTCTTGTCCTTGACTCGTATTTTGGCACATTTCGTGAGGGATATTAGAGATCCGGAGCATCCGTATTCGTTAGAGCAGCTTAGTGTTCTTTCAGAGGAATCGATCACCGTTGATGAGAAGCTAGGGCGTATCCTGATTACTTTCACTCCGACTATCCAGCATTGTAGTATGGCGACTGTGATTGGCCTCTGTCTGAGAGTGAAACTTAAACACTTCTTCCCCCCACATTACAAGGTTGACATCAAAGTGTCTCCAGGATCCCACGCAAATGAAGAGTCAGTCAATAAGCAGCTAAATGACAAAGAAAGAGTTGCAGCTGCTTTGGAGAATCCAAATCTGCGTCAGCTTGTAGATGAATGCCTGGATTCCAGTGAGTTCTGAATAAATCAAATATCATCGTACGGATGGATAAGAAAAAAGGTTGAGTTTGTATAACTTAAAGTGGGTGATGGAAATCTGGCTTGCTATGTGAGTTTATTTATTTCTGGTTTAACCAACTTCAATTTATTTAATAATTTTAATCTTTTTAAAAGACTTTTGAGTGAGTGATATGTTTGAGGATATTATTCTAAGCTAAGGGGATGATATTTTTAAGTAGAAATAGGAAAATAATGAGTTTTGGTATATGACTGTGTTATCAACTCTTTTGAGCAATTGATATTCATACCATAACCTACATGAT

Coding sequence (CDS)

ATGACGCTGGGGCTTATTAATGCGAACCCGGTGGTTCACGCCAAGAAGGAGCGGATCGCTCGCTCCGAGGACTTTCACGGCGACGACGCTGTTGATCCTCTCGAAATCTATGATATCCTTTTCTTAATTGATCGTTTTTCTCGTTTCTTTTGGAATCTGTTATTTGATAGTCAATTTGCAAATTTCTTGTCCTTGACTCGTATTTTGGCACATTTCGTGAGGGATATTAGAGATCCGGAGCATCCGTATTCGTTAGAGCAGCTTAGTGTTCTTTCAGAGGAATCGATCACCGTTGATGAGAAGCTAGGGCGTATCCTGATTACTTTCACTCCGACTATCCAGCATTGTAGTATGGCGACTGTGATTGGCCTCTGTCTGAGAGTGAAACTTAAACACTTCTTCCCCCCACATTACAAGGTTGACATCAAAGTGTCTCCAGGATCCCACGCAAATGAAGAGTCAGTCAATAAGCAGCTAAATGACAAAGAAAGAGTTGCAGCTGCTTTGGAGAATCCAAATCTGCGTCAGCTTGTAGATGAATGCCTGGATTCCAGTGAGTTCTGA

Protein sequence

MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFANFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDECLDSSEF

Homology

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BLAST of Carg05777 vs. NCBI nr
Match: XP_022984190.1 (protein AE7-like 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 293.5 bits (750), Expect = 5.1e-76
Identity = 153/187 (81.82%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARSE+FHGDDAVDPLEIYD                      
Sbjct: 1   MTLGLINANPVVHAKKERIARSEEFHGDDAVDPLEIYD---------------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT
Sbjct: 61  -----------FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 154

Query: 181 CLDSSEF 188
           CLDSSEF
Sbjct: 181 CLDSSEF 154

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BLAST of Carg05777 vs. NCBI nr
Match: XP_023551936.1 (protein AE7-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 293.1 bits (749), Expect = 6.6e-76
Identity = 153/187 (81.82%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD                      
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD---------------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT
Sbjct: 61  -----------FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALE+PNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALEDPNLRQLVDE 154

Query: 181 CLDSSEF 188
           CLDSSEF
Sbjct: 181 CLDSSEF 154

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BLAST of Carg05777 vs. NCBI nr
Match: XP_022929193.1 (protein AE7-like 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 292.0 bits (746), Expect = 1.5e-75
Identity = 153/187 (81.82%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD                      
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD---------------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT
Sbjct: 61  -----------FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKHFFPPHYKVDIKVS GSHANEESVNKQLNDKERVAAALENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHFFPPHYKVDIKVSSGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 154

Query: 181 CLDSSEF 188
           CLDSSEF
Sbjct: 181 CLDSSEF 154

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BLAST of Carg05777 vs. NCBI nr
Match: KHN36128.1 (MIP18 family protein [Glycine soja])

HSP 1 Score: 290.4 bits (742), Expect = 4.3e-75
Identity = 152/186 (81.72%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARS+D H DDAVDPL+IYDILF    FS     L   +Q  
Sbjct: 1   MTLGLINANPVVHAKKERIARSDDPHADDAVDPLDIYDILFFFFSFSL----LPLPTQLT 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
             + L   L +FVRDIRDPEHPYSLEQLSVLSEESI+VD+KLGRILITFTPT+QHCSMAT
Sbjct: 61  VEI-LDLALTNFVRDIRDPEHPYSLEQLSVLSEESISVDDKLGRILITFTPTVQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKH+FPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHYFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180

Query: 181 CLDSSE 187
           CL S+E
Sbjct: 181 CLYSNE 181

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BLAST of Carg05777 vs. NCBI nr
Match: XP_025983745.1 (uncharacterized protein LOC100500284 isoform X1 [Glycine max] >KRH76820.1 hypothetical protein GLYMA_01G175900 [Glycine max])

HSP 1 Score: 288.9 bits (738), Expect = 1.2e-74
Identity = 151/186 (81.18%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARS+D H DDAVDPL+IYDILF       F ++LL      
Sbjct: 1   MTLGLINANPVVHAKKERIARSDDPHADDAVDPLDIYDILFF------FSFSLLPLPTQL 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
               L   L +FVRDIRDPEHPYSLEQLSVLSEESI+VD+KLGRILITFTPT+QHCSMAT
Sbjct: 61  TVEILDLALTNFVRDIRDPEHPYSLEQLSVLSEESISVDDKLGRILITFTPTVQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKH+FPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHYFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180

Query: 181 CLDSSE 187
           CL S+E
Sbjct: 181 CLYSNE 180

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BLAST of Carg05777 vs. TAIR10
Match: AT3G50845.1 (Protein of unknown function (DUF59))

HSP 1 Score: 229.9 bits (585), Expect = 1.2e-60
Identity = 122/186 (65.59%), Postives = 133/186 (71.51%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVV AKKE + R ED + DD VDPLEIYD                      
Sbjct: 1   MTLGLINANPVVQAKKEGLVRREDQYRDDGVDPLEIYD---------------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      +VRDIRDPEHPY+LEQL V+SEES+TVD+KL RILITFTPTIQHCSMA 
Sbjct: 61  -----------YVRDIRDPEHPYTLEQLRVVSEESVTVDDKLDRILITFTPTIQHCSMAN 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           +IGLCLR KLK     HYKVDI+VSPGSHA+E SVNKQLNDKERV AALENPNLRQLVDE
Sbjct: 121 IIGLCLRAKLKECLQLHYKVDIRVSPGSHADEVSVNKQLNDKERVVAALENPNLRQLVDE 153

Query: 181 CLDSSE 187
           C+ S E
Sbjct: 181 CIYSDE 153

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BLAST of Carg05777 vs. TAIR10
Match: AT1G68310.1 (Protein of unknown function (DUF59))

HSP 1 Score: 174.1 bits (440), Expect = 8.1e-44
Identity = 92/190 (48.42%), Postives = 122/190 (64.21%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGD----DAVDPLEIYDILFLIDRFSRFFWNLLFD 60
           M  GLIN NP+++ KKER  R++    D    + +D LEI+DIL                
Sbjct: 1   MVSGLINENPIIYPKKERRLRTDTSITDELTPEPIDQLEIFDIL---------------- 60

Query: 61  SQFANFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHC 120
                             +I+DPEHP +LE L V++E+S+ VD++   + +TFTPT++HC
Sbjct: 61  ---------------SSSNIKDPEHPNTLEDLRVVTEDSVEVDDENSYVRVTFTPTVEHC 120

Query: 121 SMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQ 180
           SMATVIGLC+RVKL    P  YK+DI+V+PGSHA E+++NKQLNDKERVAAALENPNL +
Sbjct: 121 SMATVIGLCVRVKLLRSLPSRYKIDIRVAPGSHATEDALNKQLNDKERVAAALENPNLVE 159

Query: 181 LVDECLDSSE 187
           +VDECL S E
Sbjct: 181 MVDECLPSEE 159

[Close]

BLAST of Carg05777 vs. TAIR10
Match: AT3G09380.1 (Protein of unknown function (DUF59))

HSP 1 Score: 128.3 bits (321), Expect = 5.1e-30
Identity = 63/111 (56.76%), Postives = 82/111 (73.87%), Query Frame = 0


Query: 73  VRDIRDPEHP-YSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMATVIGLCLRVKLK 132
           +RDI+DPEHP  SLE L+VL+EES+ VD+    + ITFTPT+ HC + T IGLC+ VKL 
Sbjct: 43  IRDIKDPEHPELSLEDLNVLTEESVEVDDHKSYVRITFTPTLPHCHLPTHIGLCILVKLV 102

Query: 133 HFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDECL 183
              P  +KVD++V+PGSH  E +VNKQL DKERV AALENP L  L+++ +
Sbjct: 103 QSLPARFKVDVRVAPGSHDKETTVNKQLGDKERVTAALENPELVALLNKMM 153

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BLAST of Carg05777 vs. Swiss-Prot
Match: sp|A8MR89|AEL1_ARATH (Protein AE7-like 1 OS=Arabidopsis thaliana OX=3702 GN=AEL1 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 2.2e-59
Identity = 122/186 (65.59%), Postives = 133/186 (71.51%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVV AKKE + R ED + DD VDPLEIYD                      
Sbjct: 1   MTLGLINANPVVQAKKEGLVRREDQYRDDGVDPLEIYD---------------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      +VRDIRDPEHPY+LEQL V+SEES+TVD+KL RILITFTPTIQHCSMA 
Sbjct: 61  -----------YVRDIRDPEHPYTLEQLRVVSEESVTVDDKLDRILITFTPTIQHCSMAN 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           +IGLCLR KLK     HYKVDI+VSPGSHA+E SVNKQLNDKERV AALENPNLRQLVDE
Sbjct: 121 IIGLCLRAKLKECLQLHYKVDIRVSPGSHADEVSVNKQLNDKERVVAALENPNLRQLVDE 153

Query: 181 CLDSSE 187
           C+ S E
Sbjct: 181 CIYSDE 153

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BLAST of Carg05777 vs. Swiss-Prot
Match: sp|Q9C9G6|AE7_ARATH (Protein AE7 OS=Arabidopsis thaliana OX=3702 GN=AE7 PE=1 SV=2)

HSP 1 Score: 176.8 bits (447), Expect = 2.3e-43
Identity = 92/190 (48.42%), Postives = 122/190 (64.21%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGD----DAVDPLEIYDILFLIDRFSRFFWNLLFD 60
           M  GLIN NP+++ KKER  R++    D    + +D LEI+D                  
Sbjct: 1   MVSGLINENPIIYPKKERRLRTDTSITDELTPEPIDQLEIFD------------------ 60

Query: 61  SQFANFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHC 120
                           +RDI+DPEHP +LE L V++E+S+ VD++   + +TFTPT++HC
Sbjct: 61  ---------------HIRDIKDPEHPNTLEDLRVVTEDSVEVDDENSYVRVTFTPTVEHC 120

Query: 121 SMATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQ 180
           SMATVIGLC+RVKL    P  YK+DI+V+PGSHA E+++NKQLNDKERVAAALENPNL +
Sbjct: 121 SMATVIGLCVRVKLLRSLPSRYKIDIRVAPGSHATEDALNKQLNDKERVAAALENPNLVE 157

Query: 181 LVDECLDSSE 187
           +VDECL S E
Sbjct: 181 MVDECLPSEE 157

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BLAST of Carg05777 vs. Swiss-Prot
Match: sp|O62252|U195_CAEEL (MIP18 family protein F45G2.10 OS=Caenorhabditis elegans OX=6239 GN=F45G2.10 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-30
Identity = 79/184 (42.93%), Postives = 101/184 (54.89%), Query Frame = 0


Query: 5   LINANPVVHAKKER----IARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 64
           L NANP +   K R         D   +D +D  EI+D+                     
Sbjct: 6   LDNANPTLFDSKPRHRPVTGTERDESVEDPIDSWEIFDL--------------------- 65

Query: 65  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITV--DEKLGRILITFTPTIQHCSM 124
                       +RDI DPEHPY+LEQL+V+ EE I V  DE+   + + FTPTI HCSM
Sbjct: 66  ------------IRDINDPEHPYTLEQLNVVQEELIKVFIDEEETFVKVNFTPTIPHCSM 125

Query: 125 ATVIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLV 183
           AT+IGL +RVKL     P  KV + ++PGSH+ EES+N+QL DKERVAAA+EN  L   V
Sbjct: 126 ATLIGLAIRVKLLRSLHPKVKVSVSITPGSHSTEESINRQLADKERVAAAMENQGLMHAV 156

[Close]

BLAST of Carg05777 vs. Swiss-Prot
Match: sp|Q9VTC4|GALL2_DROME (MIP18 family protein galla-2 OS=Drosophila melanogaster OX=7227 GN=galla-2 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.7e-30
Identity = 74/179 (41.34%), Postives = 108/179 (60.34%), Query Frame = 0


Query: 7   NANPVVHAK-KERI--ARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFANFL 66
           N NP V+ + KER+  A  ED +  D  D  EI+D+                        
Sbjct: 7   NINPNVYDRIKERVLTANEEDENVPDPFDKREIFDL------------------------ 66

Query: 67  SLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMATVIG 126
                    +R+I DPEHP +LE+L V+ E+ I +++    + I+FTPTI HCSMAT+IG
Sbjct: 67  ---------IRNINDPEHPLTLEELHVVQEDLIRINDSQNSVHISFTPTIPHCSMATLIG 126

Query: 127 LCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDECL 183
           L +RVKL    PP +KV ++++PG+HA+E +VNKQL DKERVAAALEN +L +++++C+
Sbjct: 127 LSIRVKLLRSLPPRFKVTVEITPGTHASELAVNKQLADKERVAAALENNHLAEVINQCI 152

[Close]

BLAST of Carg05777 vs. Swiss-Prot
Match: sp|Q9H5X1|FA96A_HUMAN (MIP18 family protein FAM96A OS=Homo sapiens OX=9606 GN=FAM96A PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.9e-30
Identity = 62/113 (54.87%), Postives = 89/113 (78.76%), Query Frame = 0


Query: 73  VRDIRDPEHPYSLEQLSVLSEESITV---DEKLGRILITFTPTIQHCSMATVIGLCLRVK 132
           +R IRDPE P +LE+L V+SE  + V   +E+   ++I FTPT+ HCS+AT+IGLCLRVK
Sbjct: 44  IRTIRDPEKPNTLEELEVVSESCVEVQEINEEEYLVIIRFTPTVPHCSLATLIGLCLRVK 103

Query: 133 LKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDECL 183
           L+   P  +K++I +S G+H+ EE +NKQ+NDKERVAAA+ENPNLR++V++C+
Sbjct: 104 LQRCLPFKHKLEIYISEGTHSTEEDINKQINDKERVAAAMENPNLREIVEQCV 156

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BLAST of Carg05777 vs. TrEMBL
Match: tr|I1J8W2|I1J8W2_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_01G175900 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 8.2e-75
Identity = 151/186 (81.18%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARS+D H DDAVDPL+IYDILF       F ++LL      
Sbjct: 1   MTLGLINANPVVHAKKERIARSDDPHADDAVDPLDIYDILFF------FSFSLLPLPTQL 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
               L   L +FVRDIRDPEHPYSLEQLSVLSEESI+VD+KLGRILITFTPT+QHCSMAT
Sbjct: 61  TVEILDLALTNFVRDIRDPEHPYSLEQLSVLSEESISVDDKLGRILITFTPTVQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKH+FPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHYFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180

Query: 181 CLDSSE 187
           CL S+E
Sbjct: 181 CLYSNE 180

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BLAST of Carg05777 vs. TrEMBL
Match: tr|A0A1S3BWZ8|A0A1S3BWZ8_CUCME (protein AE7-like 1 OS=Cucumis melo OX=3656 GN=LOC103494387 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.1e-74
Identity = 150/186 (80.65%), Postives = 152/186 (81.72%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD                      
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD---------------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT
Sbjct: 61  -----------FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+SVNKQLNDKERVAAA+ENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDSVNKQLNDKERVAAAMENPNLRQLVDE 153

Query: 181 CLDSSE 187
           CL SSE
Sbjct: 181 CLYSSE 153

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BLAST of Carg05777 vs. TrEMBL
Match: tr|A0A0A0KUM2|A0A0A0KUM2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G038660 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.1e-74
Identity = 150/186 (80.65%), Postives = 152/186 (81.72%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD                      
Sbjct: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYD---------------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT
Sbjct: 61  -----------FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANE+SVNKQLNDKERVAAA+ENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEDSVNKQLNDKERVAAAMENPNLRQLVDE 153

Query: 181 CLDSSE 187
           CL SSE
Sbjct: 181 CLYSSE 153

[Close]

BLAST of Carg05777 vs. TrEMBL
Match: tr|A0A251NBW4|A0A251NBW4_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G154400 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.7e-72
Identity = 147/186 (79.03%), Postives = 157/186 (84.41%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAK+ER+AR+ED H DDAVDPL+IYDIL  I     FF+ + F +   
Sbjct: 1   MTLGLINANPVVHAKRERVARTEDLHADDAVDPLDIYDIL-SISHCLFFFFGIFFFT--- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                      ++ DIRDPEHPYSLEQLSVLSEESITVD+KLGRILITFTPTIQHCSMAT
Sbjct: 61  -----------WILDIRDPEHPYSLEQLSVLSEESITVDDKLGRILITFTPTIQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLKH FPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKHCFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 171

Query: 181 CLDSSE 187
           CL SSE
Sbjct: 181 CLYSSE 171

[Close]

BLAST of Carg05777 vs. TrEMBL
Match: tr|A0A1R3IPC4|A0A1R3IPC4_9ROSI (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_22083 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-71
Identity = 146/186 (78.49%), Postives = 154/186 (82.80%), Query Frame = 0


Query: 1   MTLGLINANPVVHAKKERIARSEDFHGDDAVDPLEIYDILFLIDRFSRFFWNLLFDSQFA 60
           MTLGLINANPVVHAKKER+ARSED HGDDAVDPLEIYDIL L                  
Sbjct: 1   MTLGLINANPVVHAKKERVARSEDPHGDDAVDPLEIYDILPLFS---------------- 60

Query: 61  NFLSLTRILAHFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120
                   + +FVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT
Sbjct: 61  --------ILNFVRDIRDPEHPYSLEQLSVLSEESITVDEKLGRILITFTPTIQHCSMAT 120

Query: 121 VIGLCLRVKLKHFFPPHYKVDIKVSPGSHANEESVNKQLNDKERVAAALENPNLRQLVDE 180
           VIGLCLRVKLK  FPPH+K+DIKV+PGSHA+EESVNKQLNDKERVAAALENPNLRQLVDE
Sbjct: 121 VIGLCLRVKLKECFPPHFKLDIKVTPGSHADEESVNKQLNDKERVAAALENPNLRQLVDE 162

Query: 181 CLDSSE 187
           CL S+E
Sbjct: 181 CLYSNE 162

The following BLAST results are available for this feature:

BLAST of Carg05777 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022984190.1	5.1e-76	81.82	protein AE7-like 1 isoform X2 [Cucurbita maxima]	[more]
XP_023551936.1	6.6e-76	81.82	protein AE7-like 1 [Cucurbita pepo subsp. pepo]	[more]
XP_022929193.1	1.5e-75	81.82	protein AE7-like 1 isoform X2 [Cucurbita moschata]	[more]
KHN36128.1	4.3e-75	81.72	MIP18 family protein [Glycine soja]	[more]
XP_025983745.1	1.2e-74	81.18	uncharacterized protein LOC100500284 isoform X1 [Glycine max] >KRH76820.1 hypoth...	[more]

BLAST of Carg05777 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G50845.1	1.2e-60	65.59	Protein of unknown function (DUF59)	[more]
AT1G68310.1	8.1e-44	48.42	Protein of unknown function (DUF59)	[more]
AT3G09380.1	5.1e-30	56.76	Protein of unknown function (DUF59)	[more]

BLAST of Carg05777 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|A8MR89\|AEL1_ARATH	2.2e-59	65.59	Protein AE7-like 1 OS=Arabidopsis thaliana OX=3702 GN=AEL1 PE=3 SV=1	[more]
sp\|Q9C9G6\|AE7_ARATH	2.3e-43	48.42	Protein AE7 OS=Arabidopsis thaliana OX=3702 GN=AE7 PE=1 SV=2	[more]
sp\|O62252\|U195_CAEEL	1.3e-30	42.93	MIP18 family protein F45G2.10 OS=Caenorhabditis elegans OX=6239 GN=F45G2.10 PE=1...	[more]
sp\|Q9VTC4\|GALL2_DROME	1.7e-30	41.34	MIP18 family protein galla-2 OS=Drosophila melanogaster OX=7227 GN=galla-2 PE=1 ...	[more]
sp\|Q9H5X1\|FA96A_HUMAN	4.9e-30	54.87	MIP18 family protein FAM96A OS=Homo sapiens OX=9606 GN=FAM96A PE=1 SV=1	[more]

BLAST of Carg05777 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|I1J8W2\|I1J8W2_SOYBN	8.2e-75	81.18	Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_01G175900 PE=4 SV=1	[more]
tr\|A0A1S3BWZ8\|A0A1S3BWZ8_CUCME	3.1e-74	80.65	protein AE7-like 1 OS=Cucumis melo OX=3656 GN=LOC103494387 PE=4 SV=1	[more]
tr\|A0A0A0KUM2\|A0A0A0KUM2_CUCSA	3.1e-74	80.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G038660 PE=4 SV=1	[more]
tr\|A0A251NBW4\|A0A251NBW4_PRUPE	1.7e-72	79.03	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G154400 PE=4 SV=1	[more]
tr\|A0A1R3IPC4\|A0A1R3IPC4_9ROSI	1.1e-71	78.49	Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_22083 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR034904	FSCA_dom_sf
IPR002744	MIP18-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05777-RA	Carg05777-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002744	MIP18 family-like	PFAM	PF01883	FeS_assembly_P	coord: 74..135 e-value: 1.6E-7 score: 31.3
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	G3DSA:3.30.300.130		coord: 64..183 e-value: 3.7E-49 score: 167.7
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	SSF117916	Fe-S cluster assembly (FSCA) domain-like	coord: 72..157
None	No IPR available	PANTHER	PTHR12377:SF3	PROTEIN AE7-LIKE 1	coord: 1..185
None	No IPR available	PANTHER	PTHR12377	UNCHARACTERIZED	coord: 1..185

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg05777	Cla014513	Watermelon (97103) v1	carwmB1018
Carg05777	Csa4G038660	Cucumber (Chinese Long) v2	carcuB1033
Carg05777	MELO3C017563	Melon (DHL92) v3.5.1	carmeB0992
Carg05777	ClCG07G009210	Watermelon (Charleston Gray)	carwcgB0999
Carg05777	CSPI04G04930	Wild cucumber (PI 183967)	carcpiB1057
Carg05777	Cucsa.201420	Cucumber (Gy14) v1	carcgyB1134
Carg05777	CmaCh04G007860	Cucurbita maxima (Rimu)	carcmaB1414
Carg05777	CmaCh16G006610	Cucurbita maxima (Rimu)	carcmaB1409
Carg05777	CmoCh04G008420	Cucurbita moschata (Rifu)	carcmoB1371
Carg05777	CmoCh16G007180	Cucurbita moschata (Rifu)	carcmoB1366
Carg05777	Lsi07G001970	Bottle gourd (USVL1VR-Ls)	carlsiB935
Carg05777	Cp4.1LG14g01470	Cucurbita pepo (Zucchini)	carcpeB1414
Carg05777	Cp4.1LG01g09530	Cucurbita pepo (Zucchini)	carcpeB1417
Carg05777	CsGy4G004820	Cucumber (Gy14) v2	carcgybB1005
Carg05777	MELO3C017563.2	Melon (DHL92) v3.6.1	carmedB1004
Carg05777	CsaV3_4G005030	Cucumber (Chinese Long) v3	carcucB1063
Carg05777	Cla97C07G136100	Watermelon (97103) v2	carwmbB1084
Carg05777	Bhi01G001905	Wax gourd	carwgoB1193

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg05777	Carg14182	Silver-seed gourd	carcarB646

The following block(s) are covering this gene:


Gene	Organism	Block
Carg05777	Cucurbita maxima (Rimu)	carcmaB1415
Carg05777	Cucurbita pepo (Zucchini)	carcpeB1413
Carg05777	Cucurbita pepo (Zucchini)	carcpeB1419
Carg05777	Watermelon (97103) v1	carwmB1022
Carg05777	Watermelon (97103) v2	carwmbB1073