BLAST of Carg03973 vs. NCBI nr
Match:
XP_022951936.1 (RNA pseudouridine synthase 2, chloroplastic [Cucurbita moschata])
HSP 1 Score: 844.7 bits (2181), Expect = 1.4e-241
Identity = 425/426 (99.77%), Postives = 425/426 (99.77%), Query Frame = 0
Query: 1 MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY
Sbjct: 1 MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
Query: 61 AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG
Sbjct: 61 AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
CSLPMVTTTNEELLSD DISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
Query: 421 QISTKK 427
QISTKK
Sbjct: 421 QISTKK 426
BLAST of Carg03973 vs. NCBI nr
Match:
XP_023538538.1 (RNA pseudouridine synthase 2, chloroplastic [Cucurbita pepo subsp. pepo])
HSP 1 Score: 837.8 bits (2163), Expect = 1.8e-239
Identity = 420/426 (98.59%), Postives = 424/426 (99.53%), Query Frame = 0
Query: 1 MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHS RCSGSDECGDETLPEPRTNY
Sbjct: 1 MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSARCSGSDECGDETLPEPRTNY 60
Query: 61 AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNV+AG
Sbjct: 61 AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVRAG 120
Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
DKINCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSA+SRTNNNSN
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAISRTNNNSN 300
Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGD+VYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDDVYGGTKSMAMS 360
Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
LLRTRTSSSCH+QLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSCHNQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
Query: 421 QISTKK 427
QISTKK
Sbjct: 421 QISTKK 426
BLAST of Carg03973 vs. NCBI nr
Match:
XP_023002352.1 (RNA pseudouridine synthase 2, chloroplastic [Cucurbita maxima])
HSP 1 Score: 820.5 bits (2118), Expect = 2.9e-234
Identity = 415/426 (97.42%), Postives = 420/426 (98.59%), Query Frame = 0
Query: 1 MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
MIS+CSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHS RCSGSDECG ETLPEP TNY
Sbjct: 1 MISVCSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSARCSGSDECG-ETLPEPTTNY 60
Query: 61 AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
AGMRLEEIV+T SGKVR+DSWISCRIDGISRARVQSSIRAGLVSVNGRVI+KASHNVKAG
Sbjct: 61 AGMRLEEIVKTNSGKVRIDSWISCRIDGISRARVQSSIRAGLVSVNGRVIEKASHNVKAG 120
Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
DKINCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS+
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSH 300
Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
LLRTRTSSS HSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSSHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
Query: 421 QISTKK 427
QISTKK
Sbjct: 421 QISTKK 425
BLAST of Carg03973 vs. NCBI nr
Match:
XP_022142903.1 (RNA pseudouridine synthase 2, chloroplastic isoform X1 [Momordica charantia] >XP_022142904.1 RNA pseudouridine synthase 2, chloroplastic isoform X1 [Momordica charantia])
HSP 1 Score: 732.6 bits (1890), Expect = 8.0e-208
Identity = 373/426 (87.56%), Postives = 393/426 (92.25%), Query Frame = 0
Query: 1 MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
MIS+CS RAIQAAPSIQLFFPRNRGRI+ +V RIATNHS RCS DECG E E TNY
Sbjct: 1 MISVCSVRAIQAAPSIQLFFPRNRGRITSNVHRIATNHSTRCSDYDECG-EAHSERITNY 60
Query: 61 AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
AGMRLEEIV+ SGK+RLDSWISCRI+GISRARVQSSIRAGLV+VNGRVIDK SHN+KAG
Sbjct: 61 AGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKAG 120
Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
D INCTISELQPLRA+PEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
CSLPMV T+N E LSDMDISDDE +S EILSSR SEAPIRPGIVHR+DKGTSGLLVVAKD
Sbjct: 181 CSLPMVATSNGE-LSDMDISDDEFSSNEILSSRASEAPIRPGIVHRLDKGTSGLLVVAKD 240
Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
EH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPI RDPNNRIRMSAV RTN N +
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMSAVLRTNKNPH 300
Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
AKYAASRYKVIE LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+S
Sbjct: 301 AKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMALS 360
Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
LLRTRTSSSCHSQLMQLVS+LERPCLHALTLGFIHP+TG+NIRFSCPPPTDFA+I QL
Sbjct: 361 LLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTGENIRFSCPPPTDFADIYSQLC 420
Query: 421 QISTKK 427
+I TKK
Sbjct: 421 EIGTKK 424
BLAST of Carg03973 vs. NCBI nr
Match:
XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])
HSP 1 Score: 687.6 bits (1773), Expect = 3.0e-194
Identity = 354/427 (82.90%), Postives = 375/427 (87.82%), Query Frame = 0
Query: 1 MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
MIS+CSAR IQA+P SI FFPRNR RI +VLR + N S RCS DE E E N
Sbjct: 1 MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60
Query: 61 YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
YAG RLEE V+ GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61 YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
HCSLPMV+T+ E SD D+SDDE +SIE+ SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240
Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A T NN
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300
Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
+AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420
Query: 421 RQISTKK 427
R+I TKK
Sbjct: 421 REIGTKK 425
BLAST of Carg03973 vs. TAIR10
Match:
AT1G76050.2 (Pseudouridine synthase family protein)
HSP 1 Score: 500.0 bits (1286), Expect = 1.6e-141
Identity = 246/374 (65.78%), Postives = 293/374 (78.34%), Query Frame = 0
Query: 48 CGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNG 107
C + PR N AG+R+EEIV+ GK+RLDSWIS RI+G+SRARVQSSIR GLV+VNG
Sbjct: 54 CSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNG 113
Query: 108 RVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPG 167
RV+DK SHNVK+GD++NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPG
Sbjct: 114 RVVDKVSHNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPG 173
Query: 168 NATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRI 227
N TGTLVN IL+HCSLP V +N A +RPGIVHR+
Sbjct: 174 NPTGTLVNGILHHCSLPCVDYSNXXXXXXXXXXXXXXXXXXXXXXXXYAASVRPGIVHRL 233
Query: 228 DKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRI 287
DKGT+GLLVVAKDEH+HAHL+ QFK H+IERVY+SLT GVP GRIE+PIGRD +NRI
Sbjct: 234 DKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRI 293
Query: 288 RMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLG 347
RM+A+ A++AASRYKVIE A GG +LVEWRLETGRTHQIRAHAKYMG+PLLG
Sbjct: 294 RMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLG 353
Query: 348 DEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCP 407
DEVYGGTKSMA+SLL+ R S S ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCP
Sbjct: 354 DEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCP 413
Query: 408 PPTDFAEILGQLRQ 422
PP+D AEI+G LR+
Sbjct: 414 PPSDLAEIVGLLRR 427
BLAST of Carg03973 vs. TAIR10
Match:
AT3G19440.1 (Pseudouridine synthase family protein)
HSP 1 Score: 76.6 bits (187), Expect = 4.3e-14
Identity = 63/226 (27.88%), Postives = 108/226 (47.79%), Query Frame = 0
Query: 144 IVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDE 203
++Y+D ++V+NKP + V G T S DE
Sbjct: 185 VLYKDPAIIVLNKPHGLAVQGGSGIKT-----------------------------SIDE 244
Query: 204 LNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQ---------- 263
L + L SE+ P +VHR+D+ SGLLV+A+ + A L + F++
Sbjct: 245 L-AASCLKFDKSES---PRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGV 304
Query: 264 ----HSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS--NAKYAASR 323
S++R Y++L G PP G+I P+ + + + ++ +N + ++A +
Sbjct: 305 KKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITE 364
Query: 324 YKVIENLAQGGFSLVEWRLETGRTHQIRAH-AKYMGIPLLGDEVYG 353
Y+VIE+ + G++ +E R TGR HQ+R H A+ +G P++GD YG
Sbjct: 365 YRVIES-SPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 376
BLAST of Carg03973 vs. TAIR10
Match:
AT3G52260.3 (Pseudouridine synthase family protein)
HSP 1 Score: 68.2 bits (165), Expect = 1.5e-11
Identity = 70/275 (25.45%), Postives = 122/275 (44.36%), Query Frame = 0
Query: 103 VSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIY-LDIVYEDEHVLVVNKPAHMV 162
+ ++G V+ + +++G K+ + P + EP+ Y L+++YED+ ++ +NKP+ +
Sbjct: 91 IQIDGEVVKDPNTLLRSGSKL---VYSRLPWK-EPDTPYSLEVLYEDDDLIALNKPSGLQ 150
Query: 163 VHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRP 222
V P L T +L +D + S E+P P
Sbjct: 151 VLPG----------------GLFQQRTVLTQLQWCFGKNDSYIGS--------RESP-HP 210
Query: 223 GIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQ-----------------HSIERVYISLT 282
VHR+ +GTSG+L+ AK + A L+A F + + ++Y +L
Sbjct: 211 VPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALA 270
Query: 283 CGVPPSTAGRIEVPIG--RDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLV 342
G+ I+ PIG R P + S K A S+ V+E + SLV
Sbjct: 271 DGIVEEDEVVIKQPIGVVRYPGVAQGLYVAS-----PEGKPAFSKVFVLERDREKNCSLV 330
Query: 343 EWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 358
+ +++GR HQIR H YMG PL+ ++ G++ +
Sbjct: 331 KVEIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECL 331
BLAST of Carg03973 vs. TAIR10
Match:
AT5G51140.2 (Pseudouridine synthase family protein)
HSP 1 Score: 66.6 bits (161), Expect = 4.5e-11
Identity = 69/284 (24.30%), Postives = 117/284 (41.20%), Query Frame = 0
Query: 73 SGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQP 132
+GK +D + G R ++++G + V+G ++ S+ VK+ KI +
Sbjct: 72 TGKTIVDLFAD-EFKGRPRDYYVGAVKSGRIKVDGEIV-PVSYIVKSSQKITHFLH---- 131
Query: 133 LRAEPEDIYLDIV--YEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTN 192
R EP + D+V +++ V+ V KPA + VHP T+V
Sbjct: 132 -RHEPPVMIDDVVILHQEPDVVTVCKPASVPVHPCGQYRKNTIVG--------------- 191
Query: 193 EELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQ 252
IL + + P +HR+D+ SGLL++A+ Q
Sbjct: 192 ------------------ILDAEHDLGTLFP--IHRLDRLVSGLLIIARTAAKADFFRQQ 251
Query: 253 FKQHSIERVYISLTCGVPPSTAGRIEVPI---GRDPNNRIRMSAVSRTNNNSNAKYAASR 312
+ +++ YI+ GV P ++ I G + + + S + K A ++
Sbjct: 252 IEGGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANSSGDDKKVKGKPACTK 311
Query: 313 YKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 352
+ I+ G SLV TGRTHQIR H +Y G P+ D +Y
Sbjct: 312 FTRID--TNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLY 311
BLAST of Carg03973 vs. TAIR10
Match:
AT1G56345.1 (Pseudouridine synthase family protein)
HSP 1 Score: 61.6 bits (148), Expect = 1.4e-09
Identity = 74/302 (24.50%), Postives = 108/302 (35.76%), Query Frame = 0
Query: 143 DIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDMDISDD 202
DI+YEDE+++ VNKP G A+L ++ +++E L+
Sbjct: 76 DILYEDEYLMAVNKP------------KGVYCEAVLRSAPQIVLDSSSEYHLA------- 135
Query: 203 ELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYIS 262
+R+D+ TSG++++ K A L F +H I + YI+
Sbjct: 136 ----------------------NRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIA 195
Query: 263 LTCGVPPS------------------------TAGRIEVPIG---RDPNNRIRMSAVSRT 322
L G P+ GR+ +P G RD + +V+
Sbjct: 196 LCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRV-LPGGSFVRDMETTFEVVSVNSV 255
Query: 323 NNNSNAKYAASRYKVIE---NLAQGGFSLVEWRL-----ETGRTHQIRAHAKYMGIPLLG 382
N S + V E L+ G +GRTHQIR H +Y+GIP+ G
Sbjct: 256 KNESCELEDVNHVIVAEGERELSCGXXXXXXXXXXRAFPRSGRTHQIRLHCQYLGIPIRG 315
Query: 383 DEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCP 410
D Y G RT E LHA L HP TG +I P
Sbjct: 316 DVKYHGVYEW-----NGRT--------------FEGHELHAECLSLDHPVTGDSIVIRAP 316
BLAST of Carg03973 vs. Swiss-Prot
Match:
sp|Q3ECD0|PUS2_ARATH (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)
HSP 1 Score: 500.0 bits (1286), Expect = 2.9e-140
Identity = 246/374 (65.78%), Postives = 293/374 (78.34%), Query Frame = 0
Query: 48 CGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNG 107
C + PR N AG+R+EEIV+ GK+RLDSWIS RI+G+SRARVQSSIR GLV+VNG
Sbjct: 54 CSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNG 113
Query: 108 RVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPG 167
RV+DK SHNVK+GD++NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPG
Sbjct: 114 RVVDKVSHNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPG 173
Query: 168 NATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRI 227
N TGTLVN IL+HCSLP V +N A +RPGIVHR+
Sbjct: 174 NPTGTLVNGILHHCSLPCVDYSNXXXXXXXXXXXXXXXXXXXXXXXXYAASVRPGIVHRL 233
Query: 228 DKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRI 287
DKGT+GLLVVAKDEH+HAHL+ QFK H+IERVY+SLT GVP GRIE+PIGRD +NRI
Sbjct: 234 DKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRI 293
Query: 288 RMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLG 347
RM+A+ A++AASRYKVIE A GG +LVEWRLETGRTHQIRAHAKYMG+PLLG
Sbjct: 294 RMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLG 353
Query: 348 DEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCP 407
DEVYGGTKSMA+SLL+ R S S ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCP
Sbjct: 354 DEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCP 413
Query: 408 PPTDFAEILGQLRQ 422
PP+D AEI+G LR+
Sbjct: 414 PPSDLAEIVGLLRR 427
BLAST of Carg03973 vs. Swiss-Prot
Match:
sp|Q5Z8P2|PUS2_ORYSJ (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)
HSP 1 Score: 481.1 bits (1237), Expect = 1.4e-134
Identity = 246/401 (61.35%), Postives = 296/401 (73.82%), Query Frame = 0
Query: 34 IATNHSVRCSGSDECGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRI--DGISR 93
+ +H+ RC +D + P R + G RLEE V G+ R+D+WIS R+ G+SR
Sbjct: 31 VGASHA-RCLAADANAEAVAPSRRGGHGGTRLEEAVPAGEGRSRIDAWISARLGGGGVSR 90
Query: 94 ARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHV 153
AR+Q+SIRAGLV VNGR + K SH VK GD ++CT+ ELQPLRAEPEDI LDIVYED+H+
Sbjct: 91 ARIQASIRAGLVVVNGRPVSKVSHMVKGGDIVSCTVLELQPLRAEPEDIPLDIVYEDDHL 150
Query: 154 LVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSD--MDISDDELNSIEI 213
LVVNKPAHMVVHPAPGNA GTLVNAIL+HC + T + D D SDD+++ +I
Sbjct: 151 LVVNKPAHMVVHPAPGNANGTLVNAILHHCKISTFTCLARNSIDDECPDSSDDDIDVFDI 210
Query: 214 -------LSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYIS 273
+SS V EA +RPGIVHR+DKGTSGLLVVAKDEH+HA L+ QFK H+I RVYIS
Sbjct: 211 DQFTTGEVSSEVREALVRPGIVHRLDKGTSGLLVVAKDEHSHAQLAEQFKLHTIRRVYIS 270
Query: 274 LTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLV 333
LTCG P +GRIEVPI RDPNNRIRM A + + A++AASRYKV E A GG +LV
Sbjct: 271 LTCGAPNPNSGRIEVPIARDPNNRIRMIATPGSGHR-YARHAASRYKVREVFAGGGSALV 330
Query: 334 EWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLE 393
EWRLETGRTHQIRAHAKY+GIPLLGDE YGGTKSMA+SLLR RT S H L ++S ++
Sbjct: 331 EWRLETGRTHQIRAHAKYLGIPLLGDETYGGTKSMALSLLRPRTPSRYHCDLSNMISKID 390
Query: 394 RPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQIS 424
RPCLHA LGF HPH+GK + FSCPPP DF E+L +L Q++
Sbjct: 391 RPCLHAALLGFKHPHSGKILEFSCPPPDDFTEVLNELHQVT 429
BLAST of Carg03973 vs. Swiss-Prot
Match:
sp|P50513|RLUD_ZYMMO (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)
HSP 1 Score: 215.7 bits (548), Expect = 1.1e-54
Identity = 143/350 (40.86%), Postives = 194/350 (55.43%), Query Frame = 0
Query: 77 RLDSWISCRIDGISRARVQSSIRAGLVS-VNGRVIDKASHNVKAGDKINCTISELQPLRA 136
RLD ++ I +SR R+++ I +G VS G ++ + +K+ D I +P
Sbjct: 16 RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75
Query: 137 EPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLS 196
EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN TLVNA+L HC+
Sbjct: 76 EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA------------- 135
Query: 197 DMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHS 256
+L+ I ++ RPGIVHRIDK TSGLLVVAK + AHA L+AQF HS
Sbjct: 136 ------GKLSGIGGVA--------RPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHS 195
Query: 257 IERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLA 316
I R Y ++ G PS G ++ P+ R NR +M+ VS + K A + Y++I L
Sbjct: 196 INRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIVS----DGRGKRAVTHYRMITPLK 255
Query: 317 QGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLM 376
SL+E RLETGRTHQ+R H +G LLGD VYG +K H L+
Sbjct: 256 NA--SLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSK-------------KAHHALL 315
Query: 377 QLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQISTK 426
Q + + +R LHA LGFIHP +GK + F P D ++L ++ IS +
Sbjct: 316 QSL-AFQRQALHAAHLGFIHPISGKQVDFDAEMPQDM-QLLFKMLMISNR 316
BLAST of Carg03973 vs. Swiss-Prot
Match:
sp|Q9CKA6|RLUD_PASMU (Ribosomal large subunit pseudouridine synthase D OS=Pasteurella multocida (strain Pm70) OX=272843 GN=rluD PE=3 SV=1)
HSP 1 Score: 193.7 bits (491), Expect = 4.4e-48
Identity = 118/344 (34.30%), Postives = 177/344 (51.45%), Query Frame = 0
Query: 77 RLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAE 136
RLD ++ SR+R+++ I LV VNG V++ V G++I + R E
Sbjct: 18 RLDQALAELFPDYSRSRLKTWIEQNLVHVNGDVVNVPRAKVYGGEQIEIVVEIDDDTRFE 77
Query: 137 PEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSD 196
PE++ L+IVYED+ +LV+NKP VVHP GNA+GT++NA+L H
Sbjct: 78 PENLPLNIVYEDDDILVINKPKDFVVHPGAGNASGTVLNALLYH---------------- 137
Query: 197 MDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSI 256
++E P R GIVHR+DK T+GL+V+AK A L ++ I
Sbjct: 138 --------------YPAIAEVP-RAGIVHRLDKDTTGLMVIAKTIPAQTKLVRDLQKRKI 197
Query: 257 ERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQ 316
R Y ++ CG+ + G ++ P+ R P R M+ + K A + Y+++E
Sbjct: 198 TREYEAIACGI-MTKGGMVDQPMARHPTKRTHMAV------HPMGKPAVTHYRIMERFR- 257
Query: 317 GGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQ 376
++ + RLETGRTHQIR H ++ PLLGD+ YGG R R + +LMQ
Sbjct: 258 -NYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQTYGG---------RPRPPKNASEELMQ 312
Query: 377 LVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 421
+ +R LHA+ L HP TG+ + + P PTDF E++ L+
Sbjct: 318 TLRDFKRQALHAIMLRLAHPITGELMEWHAPLPTDFVELVEALK 312
BLAST of Carg03973 vs. Swiss-Prot
Match:
sp|Q45480|YLYB_BACSU (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)
HSP 1 Score: 193.7 bits (491), Expect = 4.4e-48
Identity = 126/345 (36.52%), Postives = 176/345 (51.01%), Query Frame = 0
Query: 77 RLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAE 136
R+D +++ + SR +VQ ++ G V VNG + KA++ ++ GD++ T+ E + L
Sbjct: 16 RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75
Query: 137 PEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSD 196
LVVNKP MVVHPAPG+ TGTLVN ++ HC+
Sbjct: 76 AXXXXXXXXXXXXXXLVVNKPRGMVVHPAPGHLTGTLVNGLMAHCT-------------- 135
Query: 197 MDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSI 256
D+S +N + +RPGIVHRIDK TSGLL+VAK++ AH L Q ++
Sbjct: 136 -DLSG--INGV-----------MRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTV 195
Query: 257 ERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQ 316
R Y ++ G+ G I+ PIGRD +R M+ V+R N A + + V+E
Sbjct: 196 TRKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMT-VTRDGKN-----AVTHFHVLERFQD 255
Query: 317 GGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQ 376
F+LVE +LETGRTHQIR H KY+G PL GD YG K++
Sbjct: 256 --FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI------------------- 301
Query: 377 LVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQ 422
LHA LGF HP TG+ + F P P D AE++ LR+
Sbjct: 316 ---DFNGQALHAGVLGFDHPRTGEYVEFEAPLPEDMAELIENLRK 301
BLAST of Carg03973 vs. TrEMBL
Match:
tr|A0A1S3BCE4|A0A1S3BCE4_CUCME (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)
HSP 1 Score: 687.6 bits (1773), Expect = 2.0e-194
Identity = 354/427 (82.90%), Postives = 375/427 (87.82%), Query Frame = 0
Query: 1 MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
MIS+CSAR IQA+P SI FFPRNR RI +VLR + N S RCS DE E E N
Sbjct: 1 MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60
Query: 61 YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
YAG RLEE V+ GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61 YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
HCSLPMV+T+ E SD D+SDDE +SIE+ SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240
Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A T NN
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300
Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
+AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420
Query: 421 RQISTKK 427
R+I TKK
Sbjct: 421 REIGTKK 425
BLAST of Carg03973 vs. TrEMBL
Match:
tr|A0A1S3BD56|A0A1S3BD56_CUCME (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)
HSP 1 Score: 687.6 bits (1773), Expect = 2.0e-194
Identity = 354/427 (82.90%), Postives = 375/427 (87.82%), Query Frame = 0
Query: 1 MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
MIS+CSAR IQA+P SI FFPRNR RI +VLR + N S RCS DE E E N
Sbjct: 1 MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60
Query: 61 YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
YAG RLEE V+ GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61 YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
HCSLPMV+T+ E SD D+SDDE +SIE+ SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240
Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A T NN
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300
Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
+AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420
Query: 421 RQISTKK 427
R+I TKK
Sbjct: 421 REIGTKK 425
BLAST of Carg03973 vs. TrEMBL
Match:
tr|A0A0A0LKD7|A0A0A0LKD7_CUCSA (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)
HSP 1 Score: 687.2 bits (1772), Expect = 2.6e-194
Identity = 351/427 (82.20%), Postives = 374/427 (87.59%), Query Frame = 0
Query: 1 MISICSARAIQAAPS-IQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
MIS+CSAR IQA+PS I FFP NR RI +VLRI++N S RCS + E E N
Sbjct: 1 MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
Query: 61 YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
YAG RLEE V+ GKVRLDSWISCRI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61 YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
HCSLPMV T+ E SD D+SDDE +SI + SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240
Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A+ T NN
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNP 300
Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
+AKYAASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDF EIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQL 420
Query: 421 RQISTKK 427
R+I T+K
Sbjct: 421 REIGTQK 426
BLAST of Carg03973 vs. TrEMBL
Match:
tr|A0A2I4DLV5|A0A2I4DLV5_9ROSI (RNA pseudouridine synthase 2, chloroplastic isoform X2 OS=Juglans regia OX=51240 GN=LOC108981429 PE=4 SV=1)
HSP 1 Score: 558.5 bits (1438), Expect = 1.4e-155
Identity = 297/437 (67.96%), Postives = 341/437 (78.03%), Query Frame = 0
Query: 3 SICSARAIQAAPSIQLFFPRNRGRISYDVLRI--ATNHSVRCSGSDECGDETLPEPRTNY 62
S+ + Q++P F PRN R LRI + S + SDE E L R N+
Sbjct: 10 SLAKSFIFQSSPPFFCFSPRN-PRTPSSSLRICFVASSSSGSTNSDE-DKEDLAGRRANF 69
Query: 63 AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 122
G++LE+ V SGK+RLDSWIS RI GISRARVQSSIR+GLV+VNGRV+DK SHNVK G
Sbjct: 70 GGVQLEDTVSLNSGKLRLDSWISSRIGGISRARVQSSIRSGLVAVNGRVVDKVSHNVKTG 129
Query: 123 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 182
DK+NCTISELQPLRA PEDI LDIV+EDEHVLVVNKPAHMVVHPAPGNATGTLVN +L+H
Sbjct: 130 DKVNCTISELQPLRAVPEDIPLDIVFEDEHVLVVNKPAHMVVHPAPGNATGTLVNGVLHH 189
Query: 183 CSLPMVTTTNEELLSDM-DISDDELNSIEI-------LSSRVSEAPIRPGIVHRIDKGTS 242
CS P+V +NEE+LSD DISDDEL+S L SR S A IRPGIVHR+DKGTS
Sbjct: 190 CSPPVVAFSNEEVLSDAEDISDDELSSFSAGQNPNDGLYSRRSGASIRPGIVHRLDKGTS 249
Query: 243 GLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAV 302
GLLVVAKDEH+HA+LS QFK H+I+R+Y+SLT GVP AGR+EVPIGRD NNRIRM+A+
Sbjct: 250 GLLVVAKDEHSHANLSEQFKLHTIQRLYVSLTSGVPSPMAGRVEVPIGRDLNNRIRMAAI 309
Query: 303 SRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
+N + A++AASRYKVIE LA G +LVEWRLETGRTHQIRAHAKY+GIPLLGDEVYG
Sbjct: 310 PGSNISRQARHAASRYKVIEILAGGSSALVEWRLETGRTHQIRAHAKYLGIPLLGDEVYG 369
Query: 363 GTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDF 422
GTKSMA+SLLR RT S H QL+QLVS LERPCLHAL LGF+HPHT +N+ F CPPP DF
Sbjct: 370 GTKSMALSLLRPRTPPSRHGQLLQLVSRLERPCLHALALGFMHPHTMENVYFQCPPPADF 429
Query: 423 AEILGQLRQISTKKGMF 430
AEILGQLR+I T K F
Sbjct: 430 AEILGQLRKIGTVKHSF 444
BLAST of Carg03973 vs. TrEMBL
Match:
tr|A0A2N9FME9|A0A2N9FME9_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15953 PE=4 SV=1)
HSP 1 Score: 550.4 bits (1417), Expect = 3.7e-153
Identity = 293/435 (67.36%), Postives = 339/435 (77.93%), Query Frame = 0
Query: 1 MISIC-------SARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETL 60
M+S+C S++ Q +PS+ F R +LR T S S+E G E+L
Sbjct: 505 MLSLCYSSCSSSSSKLFQTSPSLFFPFSPRNPRFPSRLLRNFT-----ASSSNE-GQESL 564
Query: 61 PEP--RTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVID 120
R NY G+ LEE V+ KSGK+RLDSWIS RI GISRARVQSSIR+GLVSVNGR++D
Sbjct: 565 AVAVRRANYGGVTLEETVDIKSGKLRLDSWISSRISGISRARVQSSIRSGLVSVNGRIVD 624
Query: 121 KASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATG 180
K SHNVKAGDK+NCTISELQPL+AE E+I L+IVYEDEHVLVVNKPAHMVVHPAPGNA+G
Sbjct: 625 KVSHNVKAGDKVNCTISELQPLKAEAENIALNIVYEDEHVLVVNKPAHMVVHPAPGNASG 684
Query: 181 TLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGT 240
TLVN IL+HCSLP +NEE+LSD + S D+ S +S A IRPGIVHR+DKGT
Sbjct: 685 TLVNGILHHCSLPF---SNEEILSDDEFSTDQ-------SPNISAASIRPGIVHRLDKGT 744
Query: 241 SGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSA 300
SGLLVVAKDEH+HAHLS QFK H+I+RVYISLT G+P AGR+EVPIGRD NNRIRM+A
Sbjct: 745 SGLLVVAKDEHSHAHLSEQFKLHTIQRVYISLTSGMPSPVAGRVEVPIGRDLNNRIRMAA 804
Query: 301 VSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 360
+ +NN ++AASRYKVIE LA GG +LVEWRLETGRTHQIRAHAKY+GIPLLGDEVY
Sbjct: 805 IPGSNNCRQTRHAASRYKVIEILAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDEVY 864
Query: 361 GGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTD 420
GGTKSMA+SLLR RT+ S H QL+QLVS LERPCLHAL LGF HPHT +N+ FS PPP D
Sbjct: 865 GGTKSMALSLLRPRTAPSHHGQLLQLVSGLERPCLHALALGFKHPHTMENVCFSSPPPAD 923
Query: 421 FAEILGQLRQISTKK 427
FAEIL QLR+I T+K
Sbjct: 925 FAEILSQLREIGTEK 923
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022951936.1 | 1.4e-241 | 99.77 | RNA pseudouridine synthase 2, chloroplastic [Cucurbita moschata] | [more] |
XP_023538538.1 | 1.8e-239 | 98.59 | RNA pseudouridine synthase 2, chloroplastic [Cucurbita pepo subsp. pepo] | [more] |
XP_023002352.1 | 2.9e-234 | 97.42 | RNA pseudouridine synthase 2, chloroplastic [Cucurbita maxima] | [more] |
XP_022142903.1 | 8.0e-208 | 87.56 | RNA pseudouridine synthase 2, chloroplastic isoform X1 [Momordica charantia] >XP... | [more] |
XP_008445318.1 | 3.0e-194 | 82.90 | PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]... | [more] |
Match Name | E-value | Identity | Description | |
sp|Q3ECD0|PUS2_ARATH | 2.9e-140 | 65.78 | RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... | [more] |
sp|Q5Z8P2|PUS2_ORYSJ | 1.4e-134 | 61.35 | RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3... | [more] |
sp|P50513|RLUD_ZYMMO | 1.1e-54 | 40.86 | Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob... | [more] |
sp|Q9CKA6|RLUD_PASMU | 4.4e-48 | 34.30 | Ribosomal large subunit pseudouridine synthase D OS=Pasteurella multocida (strai... | [more] |
sp|Q45480|YLYB_BACSU | 4.4e-48 | 36.52 | Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168... | [more] |