MELO3C011342 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C011342 vs. Swiss-Prot
Match: PUS2_ARATH (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 9.4e-132
Identity = 258/400 (64.50%), Postives = 301/400 (75.25%), Query Frame = 1

Query: 3   SVCSARTIQ--ASPSS---IHF-FFPRNRRRIDSNVLRFSANFSS--RCSDYDEYSEAQS 62
           S+ +A++++  +SPSS   I F FFP+      SN +R S+   +   CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPKV-----SNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRVNN-AGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVSTSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAT 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM A 
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 HGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
            G      A+HAASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYG
Sbjct: 311 PGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYG 370

Query: 363 GTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           GTKSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LG
Sbjct: 371 GTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLG 398

BLAST of MELO3C011342 vs. Swiss-Prot
Match: PUS2_ORYSJ (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 5.3e-127
Identity = 244/401 (60.85%), Postives = 288/401 (71.82%), Query Frame = 1

Query: 7   ARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQS-ERIANYAGTRL 66
           A T  ASP +I        RR      R      +RC   D  +EA +  R   + GTRL
Sbjct: 2   ATTAAASPPAIATALSALLRRQRRRSSRCVGASHARCLAADANAEAVAPSRRGGHGGTRL 61

Query: 67  EETVDINPGKVRLDSWISSRING--ISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVI 126
           EE V    G+ R+D+WIS+R+ G  +SRAR+Q+SIRAGLV VNGR + KVSH VK GD++
Sbjct: 62  EEAVPAGEGRSRIDAWISARLGGGGVSRARIQASIRAGLVVVNGRPVSKVSHMVKGGDIV 121

Query: 127 NCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSL 186
           +CT+ ELQPLRAEPEDI LDIVYED+H+LVVNKPAHMVVHPAPGNA GTLVNAIL+HC +
Sbjct: 122 SCTVLELQPLRAEPEDIPLDIVYEDDHLLVVNKPAHMVVHPAPGNANGTLVNAILHHCKI 181

Query: 187 P----MVSTSTGEHQSDS-----DVSD-DEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGL 246
                +   S  +   DS     DV D D+F++ EV S V EA +RPGIVHRLDKGTSGL
Sbjct: 182 STFTCLARNSIDDECPDSSDDDIDVFDIDQFTTGEVSSEVREALVRPGIVHRLDKGTSGL 241

Query: 247 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHG 306
           LVVAKDEHSHA L+ QFK H+I RVYISLTCG P   +GRIE+PI RDPNNRIRM AT G
Sbjct: 242 LVVAKDEHSHAQLAEQFKLHTIRRVYISLTCGAPNPNSGRIEVPIARDPNNRIRMIATPG 301

Query: 307 TKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGT 366
           +  + +A+HAASRY V E+ A GGS+LVEWRLETGRTHQIRAHAKY+GIPLLGDE YGGT
Sbjct: 302 S-GHRYARHAASRYKVREVFAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDETYGGT 361

Query: 367 KSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGLEN 395
           KSMA+SLLR R  S  H  L  ++S ++RPCLHA  LG ++
Sbjct: 362 KSMALSLLRPRTPSRYHCDLSNMISKIDRPCLHAALLGFKH 401

BLAST of MELO3C011342 vs. Swiss-Prot
Match: RLUD_ZYMMO (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) GN=rluD PE=3 SV=2)

HSP 1 Score: 192.6 bits (488), Expect = 8.4e-48
Identity = 127/316 (40.19%), Postives = 171/316 (54.11%), Query Frame = 1

Query: 77  RLDSWISSRINGISRARVQSSIRAGLVSVN-GRVIDKVSHNVKAGDVINCTISELQPLRA 136
           RLD  ++S I  +SR R+++ I +G VS + G ++   +  +K+ D     I   +P   
Sbjct: 16  RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75

Query: 137 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQS 196
           EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN   TLVNA+L HC+        G+   
Sbjct: 76  EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA--------GKLSG 135

Query: 197 DSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 256
              V+                  RPGIVHR+DK TSGLLVVAK + +HA L+AQF  HSI
Sbjct: 136 IGGVA------------------RPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHSI 195

Query: 257 ERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQ 316
            R Y ++  G  PS  G ++ P+ R   NR +M       ++   K A + Y ++  L  
Sbjct: 196 NRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIV----SDGRGKRAVTHYRMITPLK- 255

Query: 317 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQ 376
             +SL+E RLETGRTHQ+R H   +G  LLGD VYG +K    +LL++ A          
Sbjct: 256 -NASLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSKKAHHALLQSLA---------- 284

Query: 377 LVSSLERPCLHALTLG 392
                +R  LHA  LG
Sbjct: 316 ----FQRQALHAAHLG 284

BLAST of MELO3C011342 vs. Swiss-Prot
Match: YLYB_BACSU (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) GN=ylyB PE=3 SV=3)

HSP 1 Score: 190.3 bits (482), Expect = 4.2e-47
Identity = 114/280 (40.71%), Postives = 156/280 (55.71%), Query Frame = 1

Query: 77  RLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAE 136
           R+D +++S  N  SR +VQ  ++ G V VNG  + K ++ ++ GD +  T+ E + L   
Sbjct: 16  RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75

Query: 137 PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSD 196
            E +DLDI YED+ VLVVNKP  MVVHPAPG+ TGTLVN ++ HC+              
Sbjct: 76  AEPMDLDIYYEDQDVLVVNKPRGMVVHPAPGHLTGTLVNGLMAHCT-------------- 135

Query: 197 SDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIE 256
                 + S I          +RPGIVHR+DK TSGLL+VAK++ +H  L  Q    ++ 
Sbjct: 136 ------DLSGI-------NGVMRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTVT 195

Query: 257 RVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQG 316
           R Y ++  G+     G I+ PI RD  +R  MT T   KN      A + ++V+E     
Sbjct: 196 RKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMTVTRDGKN------AVTHFHVLERFQD- 255

Query: 317 GSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 357
             +LVE +LETGRTHQIR H KY+G PL GD  YG  K++
Sbjct: 256 -FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI 259

BLAST of MELO3C011342 vs. Swiss-Prot
Match: Y1629_SYNY3 (Uncharacterized RNA pseudouridine synthase slr1629 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr1629 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.3e-45
Identity = 108/285 (37.89%), Postives = 157/285 (55.09%), Query Frame = 1

Query: 76  VRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRA 135
           VRLD W++ ++  +SR+R Q  I +G V  NG V    +  +K GD +   I EL PL  
Sbjct: 32  VRLDKWLAEQLPELSRSRCQKLIESGQVQRNGLVCQDKNLILKTGDRLVVNIPELIPLDV 91

Query: 136 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQS 195
             ++I LDI+YEDE ++++NKPA +VVHP PG+  GT+VNA+L HC  P ++   G    
Sbjct: 92  VAQNIPLDILYEDEQLIIINKPAGLVVHPGPGHPDGTVVNALLAHC--PDLAGIGGVQ-- 151

Query: 196 DSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 255
                                  RPGIVHRLDK T+G +VVAK E +  +L  Q K+ + 
Sbjct: 152 -----------------------RPGIVHRLDKDTTGAMVVAKTELALHNLQVQLKEKTA 211

Query: 256 ERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQ 315
            R+Y  +  G P    G + +P+ R P +R +M      K     + A + + ++E +  
Sbjct: 212 RRLYWGIVYGSPKEIQGTVNLPVGRHPGDRQKMGIVPVEKG---GREAVTHWRLLERI-- 271

Query: 316 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 361
           G  S +E++LETGRTHQIR H+K MG PL+GD +Y    S+ ++L
Sbjct: 272 GNHSWLEFQLETGRTHQIRVHSKQMGHPLVGDNLYTSPGSVNVNL 284

BLAST of MELO3C011342 vs. TrEMBL
Match: A0A0A0LKD7_CUCSA (Pseudouridine synthase OS=Cucumis sativus GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 4.9e-212
Identity = 375/392 (95.66%), Postives = 383/392 (97.70%), Query Frame = 1

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392

BLAST of MELO3C011342 vs. TrEMBL
Match: E0CQW6_VITVI (Pseudouridine synthase OS=Vitis vinifera GN=VIT_18s0001g00960 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 2.5e-139
Identity = 267/381 (70.08%), Postives = 301/381 (79.00%), Query Frame = 1

Query: 21  FPRNRRRIDS--NVLRFSANFSSRCSDYDEYSEAQSERIANYAGTRLEETV-DINP--GK 80
           FPRN +       +LR  +N +      DE   + S R  NYAG RLEETV D+N   GK
Sbjct: 23  FPRNPKSCSRVFRILRACSNST------DEPLVSVSGRRTNYAGVRLEETVVDVNSDSGK 82

Query: 81  VRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRA 140
           +RLDSWISSRI GISRARVQSSIR+GLVSVN R++DKVSH ++AGD +NCTISELQPLRA
Sbjct: 83  LRLDSWISSRIIGISRARVQSSIRSGLVSVNDRIVDKVSHVIRAGDKVNCTISELQPLRA 142

Query: 141 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQS 200
           EPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNA+GTLVN IL+HCSLP V+    + + 
Sbjct: 143 EPEDIPLDIVYEDEHVLVVNKPAHMVVHPAPGNASGTLVNGILHHCSLPTVALPNRDSEE 202

Query: 201 DSDVSDDEFSSIEVFSRVS--EAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQH 260
           D D+SDDEF+S          E  IRPGIVHRLDKGTSGLLVVAKDEHSHAHLS QFK H
Sbjct: 203 D-DISDDEFNSFAAGQNCGTCETNIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSEQFKLH 262

Query: 261 SIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEIL 320
           SI RVYISLT GVP   +GR+EIP+ RDPNNRIRMTA  G  N+  A+HAASRY V+EIL
Sbjct: 263 SIHRVYISLTSGVPTPLSGRVEIPVGRDPNNRIRMTAVPGPSNSGQARHAASRYKVIEIL 322

Query: 321 AQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQL 380
           A GGS+LVEWRLETGRTHQIRAHAKY+GIPLLGDEVYGGTK+MA+SLLR R  S+ HGQL
Sbjct: 323 AAGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDEVYGGTKNMALSLLRPRIPSSHHGQL 382

Query: 381 MQLVSSLERPCLHALTLGLEN 395
            QLVS L+RPCLHA+ LG  +
Sbjct: 383 AQLVSRLDRPCLHAVALGFNH 396

BLAST of MELO3C011342 vs. TrEMBL
Match: A0A061FCS3_THECC (Pseudouridine synthase OS=Theobroma cacao GN=TCM_034292 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.8e-134
Identity = 260/398 (65.33%), Postives = 305/398 (76.63%), Query Frame = 1

Query: 6   SARTIQASPSSIHFFFPRNRRRID---SNVLRFSANFSSRCSDYDEYSEAQSERIANYAG 65
           S +  Q SPS + F  PR  + +    S +LR  A  SS C+     ++ Q     +Y G
Sbjct: 12  STKAFQISPSFV-FLSPRYPKLLPFTLSGILRARARPSSGCA-----AKTQEPIRTSYTG 71

Query: 66  TRLEETVD-INPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGD 125
            +LEETVD    GK+RLDSWISSRI GISRARVQSSI++GLV+VNGRV+DKVSH ++ GD
Sbjct: 72  VQLEETVDGTKQGKLRLDSWISSRIQGISRARVQSSIKSGLVTVNGRVVDKVSHGLRHGD 131

Query: 126 VINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHC 185
            +NC ISELQPLRAEPEDI LDIVYED+HVLVVNKP HMVVHPAPGNATGTLVN IL+HC
Sbjct: 132 KVNCVISELQPLRAEPEDIPLDIVYEDDHVLVVNKPPHMVVHPAPGNATGTLVNGILHHC 191

Query: 186 SLPMVSTSTGEHQSDS-DVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 245
           SLP V+ S  E  SD+ DVSDD+      +   + A +RPGIVHRLDKGTSGLLVVAKDE
Sbjct: 192 SLPTVALSEKEVLSDTEDVSDDDEGFC--YGASAAASVRPGIVHRLDKGTSGLLVVAKDE 251

Query: 246 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 305
           HSH+HLS QFKQH+I+RVYISLTCGVP  +AGR++I I RD NNR+RM A  G  ++  A
Sbjct: 252 HSHSHLSEQFKQHTIQRVYISLTCGVPSPSAGRVDISIGRDSNNRVRMIAVPGLNHHGRA 311

Query: 306 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 365
           +HAASRY V+E+LA GGS+L++WRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+SL
Sbjct: 312 RHAASRYKVIEVLAGGGSALIQWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMALSL 371

Query: 366 LRTRASSNCHGQLMQLVSSLERPCLHALTLGLENKSLR 399
           LR R   N + QL +LVS LERP LHAL LG E+   R
Sbjct: 372 LRPRTPPNYNDQLSRLVSRLERPYLHALVLGFEHPHSR 401

BLAST of MELO3C011342 vs. TrEMBL
Match: A0A151TCK5_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_019388 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 7.0e-134
Identity = 256/394 (64.97%), Postives = 305/394 (77.41%), Query Frame = 1

Query: 11  QASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANYAGTRLEETVD 70
           Q SPSS  F    +      +V   +A+ S+  SDYD       ER  N++G RLEETVD
Sbjct: 25  QTSPSSSLFLSATSNTF--RSVTASAASGSAGDSDYD------GERNLNFSGARLEETVD 84

Query: 71  --INPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTIS 130
             I  GK+RLD+WISSRINGISRARVQSSI+AGLV VNGRVIDKVS NVKAGD I CTI+
Sbjct: 85  SKIGSGKLRLDNWISSRINGISRARVQSSIKAGLVHVNGRVIDKVSFNVKAGDEIKCTIA 144

Query: 131 ELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVST 190
           ELQ LRA PE+I LDIVYEDEHVLV+NKPAHMVVHPAPGN +GTLVN IL+HC+LP V  
Sbjct: 145 ELQKLRAVPENIPLDIVYEDEHVLVINKPAHMVVHPAPGNTSGTLVNGILHHCNLPNVEF 204

Query: 191 STGEHQSDSDVSDDEFSSIE--------VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 250
           S  E  SD++ SD+EF+S +        + SR+S A IRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 205 SKEEALSDTEDSDEEFNSYDSLASSCEGLHSRLSMASIRPGIVHRLDKGTSGLLVVAKDE 264

Query: 251 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 310
           HSH  LS QFKQ +I+RVY+S+T GVP   AGR+E+P+ RD NNR+RMTA  G  N+  A
Sbjct: 265 HSHMKLSEQFKQRTIKRVYVSITAGVPAPVAGRVEVPVGRDLNNRLRMTAVAGPNNSRKA 324

Query: 311 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 370
           +HAASRY V+EILA G  SLVEW+LETGRTHQIRAHAKY+G+PLLGDEVYGGTK+M +SL
Sbjct: 325 RHAASRYKVIEILAGGSCSLVEWKLETGRTHQIRAHAKYLGVPLLGDEVYGGTKNMVLSL 384

Query: 371 LRTRASSNCHGQLMQLVSSLERPCLHALTLGLEN 395
           LR R S++ H +++++VS L+RPCLHALTLG ++
Sbjct: 385 LRPRTSTSLHSKIIKMVSGLDRPCLHALTLGFQH 410

BLAST of MELO3C011342 vs. TrEMBL
Match: B9S051_RICCO (Pseudouridine synthase OS=Ricinus communis GN=RCOM_1298450 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 9.1e-134
Identity = 263/405 (64.94%), Postives = 302/405 (74.57%), Query Frame = 1

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSR-CSDYDEYSEAQSERIAN 60
           + SV     IQ+S S    FF  N RR+    +  S  F+ R C ++            N
Sbjct: 4   LYSVTLCCCIQSSSSLSLPFFSTNPRRLFPCNISLSRIFTIRSCQNH-----------TN 63

Query: 61  YAGTRLEETVDI---NPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 120
           Y G RLEETV +      K+RLDSWISSRI+GISRARVQSSI++GLVSVNGRV+DKVSHN
Sbjct: 64  YGGIRLEETVQVVHAKSVKLRLDSWISSRISGISRARVQSSIKSGLVSVNGRVVDKVSHN 123

Query: 121 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNA 180
           VK GD + CTISELQPLRAEPED+ LDIVYEDEH+LVVNKP HMVVHPAPGN TGTLVN 
Sbjct: 124 VKFGDKVTCTISELQPLRAEPEDMPLDIVYEDEHLLVVNKPPHMVVHPAPGNPTGTLVNG 183

Query: 181 ILNHCSLPMVSTSTGEHQSDS-DVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLV 240
           IL+HC+LP V+ S+ E  SD+ D SDD+  S   +S   E+ IRPGIVHRLDKGTSGLLV
Sbjct: 184 ILHHCNLPTVAISSQEVLSDAEDRSDDDERS---YSSSCESSIRPGIVHRLDKGTSGLLV 243

Query: 241 VAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTK 300
           VAKDEH+HAHLS QFKQH+I+RVYISLT GVP +  GRI+IPI RD NNRIRMTA  G  
Sbjct: 244 VAKDEHAHAHLSEQFKQHTIQRVYISLTSGVPSALTGRIDIPIGRDVNNRIRMTAIPGPI 303

Query: 301 NNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKS 360
               A+HAASR+ V+E LA GGS+LVEWRLETGRTHQIRAHAKY+GIPLLGDEVYGGT+S
Sbjct: 304 KQGRARHAASRFKVVETLAGGGSALVEWRLETGRTHQIRAHAKYIGIPLLGDEVYGGTRS 363

Query: 361 MAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGLENKSLRAH 401
           MA+SLLR R  S CH +L  L+S LERPCLHAL LG     +  H
Sbjct: 364 MALSLLRPRIPSTCHSELSLLLSKLERPCLHALALGSNQLKIACH 394

BLAST of MELO3C011342 vs. TAIR10
Match: AT1G76050.2 (AT1G76050.2 Pseudouridine synthase family protein)

HSP 1 Score: 471.5 bits (1212), Expect = 5.3e-133
Identity = 258/400 (64.50%), Postives = 301/400 (75.25%), Query Frame = 1

Query: 3   SVCSARTIQ--ASPSS---IHF-FFPRNRRRIDSNVLRFSANFSS--RCSDYDEYSEAQS 62
           S+ +A++++  +SPSS   I F FFP+      SN +R S+   +   CS     +E   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPKV-----SNFVRASSGIPNLVACSP----TEIII 70

Query: 63  ERIANYAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVS 122
            R+ N AG R+EE VD   GK+RLDSWISSRING+SRARVQSSIR GLV+VNGRV+DKVS
Sbjct: 71  PRVNN-AGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVS 130

Query: 123 HNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLV 182
           HNVK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLV
Sbjct: 131 HNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLV 190

Query: 183 NAILNHCSLPMVSTSTGEHQSDSD---VSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTS 242
           N IL+HCSLP V  S  E   DSD    SDDE  +    S    A +RPGIVHRLDKGT+
Sbjct: 191 NGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTT 250

Query: 243 GLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTAT 302
           GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIEIPI RD +NRIRM A 
Sbjct: 251 GLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAI 310

Query: 303 HGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
            G      A+HAASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYG
Sbjct: 311 PGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYG 370

Query: 363 GTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           GTKSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LG
Sbjct: 371 GTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLG 398

BLAST of MELO3C011342 vs. TAIR10
Match: AT3G19440.1 (AT3G19440.1 Pseudouridine synthase family protein)

HSP 1 Score: 72.0 bits (175), Expect = 9.3e-13
Identity = 63/226 (27.88%), Postives = 101/226 (44.69%), Query Frame = 1

Query: 144 IVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDE 203
           ++Y+D  ++V+NKP  + V    G  T                             S DE
Sbjct: 185 VLYKDPAIIVLNKPHGLAVQGGSGIKT-----------------------------SIDE 244

Query: 204 FSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ----------- 263
            ++  +    SE+P    +VHRLD+  SGLLV+A+ + +   L + F++           
Sbjct: 245 LAASCLKFDKSESP---RLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVK 304

Query: 264 ---HSIERVYISLTCGVPPSTAGRIEIPICR---DPNNRIRMTATHGTKNNPHAKHAASR 323
               S++R Y++L  G PP   G+I  P+ +   D     R+T  +        +HA + 
Sbjct: 305 KNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERIT-VNDNGELVSTQHAITE 364

Query: 324 YNVMEILAQGGSSLVEWRLETGRTHQIRAH-AKYMGIPLLGDEVYG 352
           Y V+E  +  G + +E R  TGR HQ+R H A+ +G P++GD  YG
Sbjct: 365 YRVIE-SSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 376

BLAST of MELO3C011342 vs. TAIR10
Match: AT3G52260.3 (AT3G52260.3 Pseudouridine synthase family protein)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-11
Identity = 64/273 (23.44%), Postives = 115/273 (42.12%), Query Frame = 1

Query: 103 VSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVV 162
           + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP+ + V
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQV 150

Query: 163 HPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGI 222
            P                  L  +    G++ S             + SR S  P+    
Sbjct: 151 LPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHPVP--- 210

Query: 223 VHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYISLTCG 282
           VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y +L  G
Sbjct: 211 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 270

Query: 283 VPPSTAGRIEIPI--CRDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGSSLVEW 342
           +       I+ PI   R P     +        +P  K A S+  V+E   +   SLV+ 
Sbjct: 271 IVEEDEVVIKQPIGVVRYPGVAQGLYVA-----SPEGKPAFSKVFVLERDREKNCSLVKV 330

Query: 343 RLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 357
            +++GR HQIR H  YMG PL+   ++ G++ +
Sbjct: 331 EIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECL 331

BLAST of MELO3C011342 vs. TAIR10
Match: AT5G51140.2 (AT5G51140.2 Pseudouridine synthase family protein)

HSP 1 Score: 66.6 bits (161), Expect = 3.9e-11
Identity = 71/282 (25.18%), Postives = 119/282 (42.20%), Query Frame = 1

Query: 74  GKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPL 133
           GK  +D + +    G  R     ++++G + V+G ++  VS+ VK+   I   +   +P 
Sbjct: 73  GKTIVDLF-ADEFKGRPRDYYVGAVKSGRIKVDGEIVP-VSYIVKSSQKITHFLHRHEP- 132

Query: 134 RAEPEDID-LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVSTSTGE 193
              P  ID + I++++  V+ V KPA + VHP                C     +T  G 
Sbjct: 133 ---PVMIDDVVILHQEPDVVTVCKPASVPVHP----------------CGQYRKNTIVGI 192

Query: 194 HQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ 253
             ++ D+         +F            +HRLD+  SGLL++A+          Q + 
Sbjct: 193 LDAEHDLGT-------LFP-----------IHRLDRLVSGLLIIARTAAKADFFRQQIEG 252

Query: 254 HSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTA----THGTKNNPHAKHAASRYN 313
             +++ YI+   GV P     ++  I  + +   R TA    + G       K A +++ 
Sbjct: 253 GMVKKRYIAKVIGVFPEDEMIVDANINYNGSEG-RSTAEDANSSGDDKKVKGKPACTKFT 311

Query: 314 VMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 351
            ++    G  SLV     TGRTHQIR H +Y G P+  D +Y
Sbjct: 313 RID--TNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLY 311

BLAST of MELO3C011342 vs. TAIR10
Match: AT1G78910.1 (AT1G78910.1 Pseudouridine synthase family protein)

HSP 1 Score: 58.2 bits (139), Expect = 1.4e-08
Identity = 46/151 (30.46%), Postives = 69/151 (45.70%), Query Frame = 1

Query: 220 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQH---------------SIERVYISLTC 279
           P +VHRLD+ TSGLLV+ + + S  +L + F  +               ++ + Y +L  
Sbjct: 225 PRLVHRLDRETSGLLVMGRTKESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVI 284

Query: 280 GVPPSTAGRIEIPICR---DPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGSSLV 339
           G P    G I  P+ +   D     R+    G+     ++ A + Y V+      G S V
Sbjct: 285 GSPKEKEGLISAPLSKVLLDDGKTDRVVLAQGS-GFEASQDAITEYKVLGPKI-NGCSWV 344

Query: 340 EWRLETGRTHQIRAH-AKYMGIPLLGDEVYG 352
           E R  T R HQ+R H A+ +G P++GD  YG
Sbjct: 345 ELRPITSRKHQLRVHCAEALGTPIVGDYKYG 373

BLAST of MELO3C011342 vs. NCBI nr
Match: gi|659089085|ref|XP_008445320.1| (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 780.8 bits (2015), Expect = 1.2e-222
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 1

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           LRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 391

BLAST of MELO3C011342 vs. NCBI nr
Match: gi|659089081|ref|XP_008445318.1| (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 780.8 bits (2015), Expect = 1.2e-222
Identity = 391/391 (100.00%), Postives = 391/391 (100.00%), Query Frame = 1

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60
           MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEYSEAQSERIANY 60

Query: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120
           AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG
Sbjct: 61  AGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAG 120

Query: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240
           CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE
Sbjct: 181 CSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDE 240

Query: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300
           HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA
Sbjct: 241 HSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPHA 300

Query: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360
           KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL
Sbjct: 301 KHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSL 360

Query: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           LRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LRTRASSNCHGQLMQLVSSLERPCLHALTLG 391

BLAST of MELO3C011342 vs. NCBI nr
Match: gi|778670398|ref|XP_011649466.1| (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 745.0 bits (1922), Expect = 7.0e-212
Identity = 375/392 (95.66%), Postives = 383/392 (97.70%), Query Frame = 1

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392

BLAST of MELO3C011342 vs. NCBI nr
Match: gi|778670396|ref|XP_011649465.1| (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 745.0 bits (1922), Expect = 7.0e-212
Identity = 375/392 (95.66%), Postives = 383/392 (97.70%), Query Frame = 1

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392

BLAST of MELO3C011342 vs. NCBI nr
Match: gi|778670400|ref|XP_011649467.1| (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 628.2 bits (1619), Expect = 9.6e-177
Identity = 316/333 (94.89%), Postives = 324/333 (97.30%), Query Frame = 1

Query: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQ 333
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQ
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQ 333