Carg03973-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg03973-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Pseudouridine synthase
Location	Cucurbita_argyrosperma_scaffold_011 : 1119803 .. 1125476 (-)
Sequence length	1586

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAACAAAATCTTAATTTTTCCCGGTAAGCAGCAGCCGATAGGCGAAAACCCAAGAACCAAATATCGAACTCGGGAGAATGATTTCCATTTGCTCTGCTAGAGCAATTCAAGCTGCACCTTCAATTCAGTTGTTTTTCCCTCGAAACCGTGGAAGAATCTCTTATGATGTTCTTCGAATCGCTACCAATCACTCTGTCAGATGCTCGGGTTCCGATGAGTGTGGTGATGAAACTCTTCCTGAACCGAGAACCAATTACGCCGGAATGAGACTGGAAGAGATCGTGGAGACAAAATCCGGTAAAGTTCGTCTTGATTCTTGGATATCCTGCCGCATCGACGGGATTAGTAGGGCTCGGGTGCAGTCCAGTATTAGGGCCGGACTGGTTTCAGTCAATGGCCGAGTTATCGATAAGGTAACGACGATTTTAGCTTGTACTCCCGAAGAACAACTGCATTTATGACTCTTCGATTGTCTCTCTGTTTTTCATTTGGCTTCTTTCAACCATATATTTTATGCTCGAGCAGGCTTCTCACAATGTTAAAGCTGGGGATAAGATTAATTGTACAATTTCGGAATTGCAGCCATTGAGAGCTGAACCTGAAGATATTTATTTGGATATAGTTTATGAGGATGAACATGTTTTAGTGGTAAACAAACCGGCGCACATGGTAATGATTCAAGAGACGTTATGCCTCAAGTTCGATATTGTATGCTTAGTGGCAAGCTTGTGTCTGAAATTTGTAAAAGATTGTGATAGTGGTTTGATTTCTCCTTGATTCAGGTTGTTCATCCAGCTCCTGGGAATGCTACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCAATGGTTACAACGACAAATGAGGAACTTCTGTCAGACATGGATATTTCTGATGATGAGCTCAATTCCATTGAGATTTTATCTTCTAGAGTTTCTGAGGCACCTATTCGTCCTGGAATTGTGCATAGGATAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGTGATTTACTGTCCCTCCTTCAGCTTCCTTGCAGCTGCTTAACTCAAGATCAAAGCAAAACAATTCAAGTTTTGTGTTTCAAGAATTTGCAGCCCAATAGCTCCCCTTTTTGTCCCCTAACTATATCTAACTCCTACGTTTGTTGCATTTGAATATCAGGATGAACATGCCCACGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTCTATATCAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGACGTATCGAGGTACCAATAGGCCGTGATCCGAATAATCGTATTCGCATGAGTGCAGTATCTAGAACAAATAACAATTCAAATGCAAAGTACGCTGCTAGTAGGTAATTAGTTTTTCTTCGTCAATTATTATCATATGAAATGCAATTATAAATCTACTGGTTCTTGAAATGCAGTCTTCCCCATGTTACTGTTGAGGATTATTGGGATTGGGAGTGTCCCACATTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGAGGAATACTACCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGATTCCTAACATGGTATCAGAGCCATGCCCTAAACCTAACCATGCCAATAGAATCCTCAAATATCGAACAAAGAATGGCGAGCCTCGAAGGTATAGTCAAAAGTGACTAAAGTGTCGAACAAATGGTGTACTTTGTTCGAGGGCTCTAGAGAAAGGAGTCGAGCCTCAATTAAGGGGAGGCTATTCGAGAGCTCCATAAGCCTCAGGGGAGGCTTATAGTGTGCTTTGTTCGAGGGGAGGTGTTGAGGATTATGGGTGTCCATTGGTGGGTTCTGTTTCCTGTAAAAAAATCATCTTTGTGGAGAGCTCCATCATTGCGTATTGGACAATTATTTTAACCATGTGGAAGACAGGTCATGTGTATTCATAACAGTAAGGAATACTACCCTGTCTACATATAAGGTCAGAACTAAAAGGTAATGATAATATACTTGTCATCATCATAAATTTGGATAACTAATATTTAGAAAGAAAACTCCACATTTCCGACAGACTGCTCAAACTCTTGGCTGAACTGATTGTTTATATGAAGAGGTTAAGGTGGTGCTTCTAGAGACATTGTCATGTTGGTGGCTTAAAGGCAAAGCTAGGACTATTTGAAAGAATGCAACTTGAGCCATCCTTTGAAGTATATAAATAGAAAGGAATAACATAATTTTCAAAGAGAGTAACAAAGCAGTAAGCTTTGGAGTAAATAAATAGAAAGGAATTATAGAATGTTCAAATAGAGTGGTAAAATTTTGGTTGGGTGACTGTATAGTACACTGCCTCCTCATGTACCAAATTTCCATAAGAATTTTGATTGGGGTCCTGTACTCTTGTAATGGATCCCCCCTTGTGCTCTTCAGGAGGAGATGCCTCTTCTCCTTGCACATTTTTTGTATTCTCTTCTGAAAAAAGATATATTCATCTCTCCCCCTCAAACTAGTTTGCGAGATGCATCTCATCCAACAAAGTCATGCAAAATTTGGAAGAAGTAATCTCTGGGATTTTCATTTCCATCCTTGTCCCATCTCTCATTATGGTGACATCAATATGAATTTAAGGATTAGTGGAGAAAGATCTTTTGTTTTTTGTGTTTACTTAGTTATTTTTCGATTATAGAAACTATTTTAGTATTATTAAGCTTGGTCATTCATTATGCTAAGTTGAAATGGCGTACATTTCACAGGTACAAAGTTATTGAAAATTTAGCTCAAGGAGGTTTTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGGTGCGTATTGTAAACTTCTTGCTCTTTAAGTTTCTTCAAAGGGCTATAAATATATATTACAATCCATCTAACAATGCATTTTAACCAATCACCATGTATGCACGTGCAAAGACATATATATTCTTTTGTTTCAATAACATTTCTGTACGCTACTGCTTTTGTTTTCAAGTCATAGTGTGTGCATACGTACGCTAAACAATTTAATGAGTCAGAGTTACACCTTGAACGTACAAGCAGCCATATTTATCATGCCAAGTTGCAGTTCTTTCCACTTTCTGAAAATTGAAATGTAGTTTTTGAATATTTGTGAAGTAGATCCGTGCACATGCGAAGTATATGGGAATACCCTTACTTGGAGATGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGGACTCGAACATCATCTAGTTGTCACAGCCAACTTATGCAACTTGTTTCCTCATTAGAGAGGCCTTGCCTTCATGCTCTAACTCTCGGGTTAGAAAATAAATTCTTAAGAGCTCGTTTAGTTACATTCCTTTCTTGTTCTCTAAAATAGTTTATAATTTTGGTTTCTTATTTCAAAGTTTTCAAAACTTGATGGAGTTTCTATTTTTCTTATTTTTCGCTAATAATTAACCAAAACATTGCTATGACATATATTTGGGATATCTTGTTAAATTTTCAGTTAAAACTTCCACATTTTAAAAGAAAATTTTTAGAGAAAGATAAACAAGAATGATTGCTAATCACAGTCTGTTCTTTTTTAAACTAAAAAGTGAGTGCAAAAGCAAGAATTGTTATAAAATTTTGTTTTTTTTTATTTCTTATGATAAAAGAGCCAGAAATAAAAAGTGAGAAACAAGAAACAAGATCGTAGTATGAGTCTTGGATTGTTCTTTTCTTCTGTAAACAACTAATCCAAGATCACTGAACAATTTGTAGGTTTATACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCTACAGATTTTGCTGAAATTCTGGGTCAGCTTCGTCAAATTAGCACAAAAAAGGTATCTCTCAAGTATTGATGAATTCTGTTATCTTTTACTCAACCCAAGCTCTTTTCATGGTGATTAGTGGTTTTACGGATTTAGCATTTTATCCATAAAATAGAACAATATCAATCAAGAATGGTCCGGGGGCTACGATTTACACACCCAACCTACTTTATGGATAACCTAGATAGCTAATTCGTGCATTTGTGCCTCTTTTTTGGTCGCCTCCTTGACACGATAGTTAATACCATGTCTTTTCTATTTCCTAGCCATGTGGTACCTGCTTCTTGCGTTTGAATGTCTATTTTATAGGTTACTTTCCTTTTGTATTTCATCTCTTACTTATGAAGTCGACTGAACTTAAAAGAAAATCAAGTTGTTTAATGAATGAGTGAGGATTTAGTCATACATATTCAAAGTTTCGAATGGTTTTTTGAGATCCCACATCAGTTGAGGAGGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCTATCCCTAGCAGACGTGTTTTAAAACCTTGAGGGGAAGCTCAGAAGGGAAAGCCCAAAGAGAACAATATCTGTTAGTGGTGGGCTTGGGCTTGGGCTATTTCAAATGGTATTAGAGCCAGTCACCGGGCGGTGTGCCAGCGAGGACGTTGGGCCCCCAAGGGGAGTGGATTATGAGATCTCACATTCGATTGGAGAGGGGAACGAAACATTCCTTATATAAGTGGGGAAACCTCTCCCTAGCAGACGTGTTTTAAAACCTTGAGGGGAAGCTTAGAAGGGAAAGTCCAAAGAGGACAATATCGGCTAGCGGTGGACTTGGGTTGTTACAAATGGAATTAAAGCTAGTCACCGGGCGGTGTGCCAGCGAGGACGCTAGGCCCCCAAGGGGAGTGGATTGTGCGATCCCACATTAGTTGGAGAGGGGAACAAAACATTCCTTATATAGGTGTGGAAACCTCTCCCTAACAAACGCGTTCTAAAACTTTGAGGGGAAGCTCAGAAGGGAAAACCCAAAAAAGACAATATCTGCCGGTGGTAGGCTTGAGCTGTTACGATAGATATAATACAAAAACTAAAGAGCAGACAGAAAACATGAAGTCTTCCTTCCAGTCGCCTCTTATACGATATGAGGCAATTTCATATCATTGTTTAATCTCTTGTAGTCCTAATACATAGTACACATTGAAATAATTTTGAGATTTACAACCACATTAGCTATTGAACAATGGTTGCTGCCATAGAATTTAGGCGGGAGAGATGGTTTTTGCGGTGTATCAGTTTAACCATTAACAGAGTGTTGTGATTAAAATTGCAGGTTACCAAATCTTAATTGGGATCCTTCAGTATCTAGCATACACACTCAGCGTCAAACGACTCGGGCATGAAAGACCGAGAAGGTACATTTGGCCCTAACATACGACCATTTAAGTTGGGACGTTTGGAAAATAAATTGAAGTTTTTGGTAAATGACATATTTTTGGTTGGTGTTCTTGGAAATTCAACACTGCTAATTTGATCTGATATGAACAGGGAATGTTCCTAAACTCAATATCCCAATATCGAAATCGAGGCATGGCAGCACGCAACAATGGCATTAGAAATGGAAACGACACACCGTATCGGTACACTTGATGGTGCTTCTACAGTCGAGATGATCGTGTTGCTTGTGTTGAACATTCGCCTAATCGCCATGTCCAATGGAACCTCATGTTTCACTTAATATTGATGGTAAGCCATCCACCATTGCTATTAATCCATA

mRNA sequence

AAAAACAAAATCTTAATTTTTCCCGGTAAGCAGCAGCCGATAGGCGAAAACCCAAGAACCAAATATCGAACTCGGGAGAATGATTTCCATTTGCTCTGCTAGAGCAATTCAAGCTGCACCTTCAATTCAGTTGTTTTTCCCTCGAAACCGTGGAAGAATCTCTTATGATGTTCTTCGAATCGCTACCAATCACTCTGTCAGATGCTCGGGTTCCGATGAGTGTGGTGATGAAACTCTTCCTGAACCGAGAACCAATTACGCCGGAATGAGACTGGAAGAGATCGTGGAGACAAAATCCGGTAAAGTTCGTCTTGATTCTTGGATATCCTGCCGCATCGACGGGATTAGTAGGGCTCGGGTGCAGTCCAGTATTAGGGCCGGACTGGTTTCAGTCAATGGCCGAGTTATCGATAAGGCTTCTCACAATGTTAAAGCTGGGGATAAGATTAATTGTACAATTTCGGAATTGCAGCCATTGAGAGCTGAACCTGAAGATATTTATTTGGATATAGTTTATGAGGATGAACATGTTTTAGTGGTAAACAAACCGGCGCACATGGTTGTTCATCCAGCTCCTGGGAATGCTACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCAATGGTTACAACGACAAATGAGGAACTTCTGTCAGACATGGATATTTCTGATGATGAGCTCAATTCCATTGAGATTTTATCTTCTAGAGTTTCTGAGGCACCTATTCGTCCTGGAATTGTGCATAGGATAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATGCCCACGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTCTATATCAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGACGTATCGAGGTACCAATAGGCCGTGATCCGAATAATCGTATTCGCATGAGTGCAGTATCTAGAACAAATAACAATTCAAATGCAAAGTACGCTGCTAGTAGGTACAAAGTTATTGAAAATTTAGCTCAAGGAGGTTTTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGATCCGTGCACATGCGAAGTATATGGGAATACCCTTACTTGGAGATGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGGACTCGAACATCATCTAGTTGTCACAGCCAACTTATGCAACTTGTTTCCTCATTAGAGAGGCCTTGCCTTCATGCTCTAACTCTCGGGTTTATACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCTACAGATTTTGCTGAAATTCTGGGTCAGCTTCGTCAAATTAGCACAAAAAAGGGAATGTTCCTAAACTCAATATCCCAATATCGAAATCGAGGCATGGCAGCACGCAACAATGGCATTAGAAATGGAAACGACACACCGTATCGGTACACTTGATGGTGCTTCTACAGTCGAGATGATCGTGTTGCTTGTGTTGAACATTCGCCTAATCGCCATGTCCAATGGAACCTCATGTTTCACTTAATATTGATGGTAAGCCATCCACCATTGCTATTAATCCATA

Coding sequence (CDS)

ATGATTTCCATTTGCTCTGCTAGAGCAATTCAAGCTGCACCTTCAATTCAGTTGTTTTTCCCTCGAAACCGTGGAAGAATCTCTTATGATGTTCTTCGAATCGCTACCAATCACTCTGTCAGATGCTCGGGTTCCGATGAGTGTGGTGATGAAACTCTTCCTGAACCGAGAACCAATTACGCCGGAATGAGACTGGAAGAGATCGTGGAGACAAAATCCGGTAAAGTTCGTCTTGATTCTTGGATATCCTGCCGCATCGACGGGATTAGTAGGGCTCGGGTGCAGTCCAGTATTAGGGCCGGACTGGTTTCAGTCAATGGCCGAGTTATCGATAAGGCTTCTCACAATGTTAAAGCTGGGGATAAGATTAATTGTACAATTTCGGAATTGCAGCCATTGAGAGCTGAACCTGAAGATATTTATTTGGATATAGTTTATGAGGATGAACATGTTTTAGTGGTAAACAAACCGGCGCACATGGTTGTTCATCCAGCTCCTGGGAATGCTACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCAATGGTTACAACGACAAATGAGGAACTTCTGTCAGACATGGATATTTCTGATGATGAGCTCAATTCCATTGAGATTTTATCTTCTAGAGTTTCTGAGGCACCTATTCGTCCTGGAATTGTGCATAGGATAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATGCCCACGCTCACTTATCTGCTCAATTCAAGCAACATTCTATTGAGAGAGTCTATATCAGTCTCACATGTGGAGTTCCACCCTCAACTGCTGGACGTATCGAGGTACCAATAGGCCGTGATCCGAATAATCGTATTCGCATGAGTGCAGTATCTAGAACAAATAACAATTCAAATGCAAAGTACGCTGCTAGTAGGTACAAAGTTATTGAAAATTTAGCTCAAGGAGGTTTTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGATCCGTGCACATGCGAAGTATATGGGAATACCCTTACTTGGAGATGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGGACTCGAACATCATCTAGTTGTCACAGCCAACTTATGCAACTTGTTTCCTCATTAGAGAGGCCTTGCCTTCATGCTCTAACTCTCGGGTTTATACATCCTCACACAGGGAAGAACATACGATTTTCATGCCCACCCCCTACAGATTTTGCTGAAATTCTGGGTCAGCTTCGTCAAATTAGCACAAAAAAGGGAATGTTCCTAAACTCAATATCCCAATATCGAAATCGAGGCATGGCAGCACGCAACAATGGCATTAGAAATGGAAACGACACACCGTATCGGTACACTTGA

Protein sequence

MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQISTKKGMFLNSISQYRNRGMAARNNGIRNGNDTPYRYT

Homology

[Close]

BLAST of Carg03973-RA vs. NCBI nr
Match: XP_022951936.1 (RNA pseudouridine synthase 2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 844.7 bits (2181), Expect = 1.4e-241
Identity = 425/426 (99.77%), Postives = 425/426 (99.77%), Query Frame = 0


Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY
Sbjct: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
           AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG
Sbjct: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120

Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
           CSLPMVTTTNEELLSD DISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDTDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240

Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
           EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300

Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
           LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420

Query: 421 QISTKK 427
           QISTKK
Sbjct: 421 QISTKK 426

[Close]

BLAST of Carg03973-RA vs. NCBI nr
Match: XP_023538538.1 (RNA pseudouridine synthase 2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 837.8 bits (2163), Expect = 1.8e-239
Identity = 420/426 (98.59%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHS RCSGSDECGDETLPEPRTNY
Sbjct: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSARCSGSDECGDETLPEPRTNY 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
           AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNV+AG
Sbjct: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVRAG 120

Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DKINCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
           CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240

Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
           EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSA+SRTNNNSN
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAISRTNNNSN 300

Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGD+VYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDDVYGGTKSMAMS 360

Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
           LLRTRTSSSCH+QLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSCHNQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420

Query: 421 QISTKK 427
           QISTKK
Sbjct: 421 QISTKK 426

[Close]

BLAST of Carg03973-RA vs. NCBI nr
Match: XP_023002352.1 (RNA pseudouridine synthase 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 820.5 bits (2118), Expect = 2.9e-234
Identity = 415/426 (97.42%), Postives = 420/426 (98.59%), Query Frame = 0


Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           MIS+CSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHS RCSGSDECG ETLPEP TNY
Sbjct: 1   MISVCSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSARCSGSDECG-ETLPEPTTNY 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
           AGMRLEEIV+T SGKVR+DSWISCRIDGISRARVQSSIRAGLVSVNGRVI+KASHNVKAG
Sbjct: 61  AGMRLEEIVKTNSGKVRIDSWISCRIDGISRARVQSSIRAGLVSVNGRVIEKASHNVKAG 120

Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           DKINCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DKINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
           CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD
Sbjct: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240

Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
           EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS+
Sbjct: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSH 300

Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
           LLRTRTSSS HSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR
Sbjct: 361 LLRTRTSSSSHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420

Query: 421 QISTKK 427
           QISTKK
Sbjct: 421 QISTKK 425

[Close]

BLAST of Carg03973-RA vs. NCBI nr
Match: XP_022142903.1 (RNA pseudouridine synthase 2, chloroplastic isoform X1 [Momordica charantia] >XP_022142904.1 RNA pseudouridine synthase 2, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 732.6 bits (1890), Expect = 8.0e-208
Identity = 373/426 (87.56%), Postives = 393/426 (92.25%), Query Frame = 0


Query: 1   MISICSARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTNY 60
           MIS+CS RAIQAAPSIQLFFPRNRGRI+ +V RIATNHS RCS  DECG E   E  TNY
Sbjct: 1   MISVCSVRAIQAAPSIQLFFPRNRGRITSNVHRIATNHSTRCSDYDECG-EAHSERITNY 60

Query: 61  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 120
           AGMRLEEIV+  SGK+RLDSWISCRI+GISRARVQSSIRAGLV+VNGRVIDK SHN+KAG
Sbjct: 61  AGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKAG 120

Query: 121 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180
           D INCTISELQPLRA+PEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH
Sbjct: 121 DMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 180

Query: 181 CSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKD 240
           CSLPMV T+N E LSDMDISDDE +S EILSSR SEAPIRPGIVHR+DKGTSGLLVVAKD
Sbjct: 181 CSLPMVATSNGE-LSDMDISDDEFSSNEILSSRASEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSN 300
           EH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPI RDPNNRIRMSAV RTN N +
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMSAVLRTNKNPH 300

Query: 301 AKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYKVIE LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+S
Sbjct: 301 AKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMALS 360

Query: 361 LLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 420
           LLRTRTSSSCHSQLMQLVS+LERPCLHALTLGFIHP+TG+NIRFSCPPPTDFA+I  QL 
Sbjct: 361 LLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTGENIRFSCPPPTDFADIYSQLC 420

Query: 421 QISTKK 427
           +I TKK
Sbjct: 421 EIGTKK 424

[Close]

BLAST of Carg03973-RA vs. NCBI nr
Match: XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 687.6 bits (1773), Expect = 3.0e-194
Identity = 354/427 (82.90%), Postives = 375/427 (87.82%), Query Frame = 0


Query: 1   MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
           MIS+CSAR IQA+P SI  FFPRNR RI  +VLR + N S RCS  DE   E   E   N
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60

Query: 61  YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
           YAG RLEE V+   GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
           HCSLPMV+T+  E  SD D+SDDE +SIE+  SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
           DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A   T NN 
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300

Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           +AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360

Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
           SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420

Query: 421 RQISTKK 427
           R+I TKK
Sbjct: 421 REIGTKK 425

[Close]

BLAST of Carg03973-RA vs. TAIR10
Match: AT1G76050.2 (Pseudouridine synthase family protein)

HSP 1 Score: 500.0 bits (1286), Expect = 1.6e-141
Identity = 246/374 (65.78%), Postives = 293/374 (78.34%), Query Frame = 0


Query: 48  CGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNG 107
           C    +  PR N AG+R+EEIV+   GK+RLDSWIS RI+G+SRARVQSSIR GLV+VNG
Sbjct: 54  CSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNG 113

Query: 108 RVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPG 167
           RV+DK SHNVK+GD++NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPG
Sbjct: 114 RVVDKVSHNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPG 173

Query: 168 NATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRI 227
           N TGTLVN IL+HCSLP V  +N                          A +RPGIVHR+
Sbjct: 174 NPTGTLVNGILHHCSLPCVDYSNXXXXXXXXXXXXXXXXXXXXXXXXYAASVRPGIVHRL 233

Query: 228 DKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRI 287
           DKGT+GLLVVAKDEH+HAHL+ QFK H+IERVY+SLT GVP    GRIE+PIGRD +NRI
Sbjct: 234 DKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRI 293

Query: 288 RMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLG 347
           RM+A+        A++AASRYKVIE  A GG +LVEWRLETGRTHQIRAHAKYMG+PLLG
Sbjct: 294 RMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLG 353

Query: 348 DEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCP 407
           DEVYGGTKSMA+SLL+ R S S   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCP
Sbjct: 354 DEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCP 413

Query: 408 PPTDFAEILGQLRQ 422
           PP+D AEI+G LR+
Sbjct: 414 PPSDLAEIVGLLRR 427

[Close]

BLAST of Carg03973-RA vs. TAIR10
Match: AT3G19440.1 (Pseudouridine synthase family protein)

HSP 1 Score: 76.6 bits (187), Expect = 4.3e-14
Identity = 63/226 (27.88%), Postives = 108/226 (47.79%), Query Frame = 0


Query: 144 IVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDE 203
           ++Y+D  ++V+NKP  + V    G  T                             S DE
Sbjct: 185 VLYKDPAIIVLNKPHGLAVQGGSGIKT-----------------------------SIDE 244

Query: 204 LNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQ---------- 263
           L +   L    SE+   P +VHR+D+  SGLLV+A+ + A   L + F++          
Sbjct: 245 L-AASCLKFDKSES---PRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGV 304

Query: 264 ----HSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS--NAKYAASR 323
                S++R Y++L  G PP   G+I  P+ +   +  +   ++  +N    + ++A + 
Sbjct: 305 KKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITE 364

Query: 324 YKVIENLAQGGFSLVEWRLETGRTHQIRAH-AKYMGIPLLGDEVYG 353
           Y+VIE+ +  G++ +E R  TGR HQ+R H A+ +G P++GD  YG
Sbjct: 365 YRVIES-SPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYG 376

[Close]

BLAST of Carg03973-RA vs. TAIR10
Match: AT3G52260.3 (Pseudouridine synthase family protein)

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-11
Identity = 70/275 (25.45%), Postives = 122/275 (44.36%), Query Frame = 0


Query: 103 VSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIY-LDIVYEDEHVLVVNKPAHMV 162
           + ++G V+   +  +++G K+   +    P + EP+  Y L+++YED+ ++ +NKP+ + 
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKL---VYSRLPWK-EPDTPYSLEVLYEDDDLIALNKPSGLQ 150

Query: 163 VHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRP 222
           V P                  L    T   +L      +D  + S         E+P  P
Sbjct: 151 VLPG----------------GLFQQRTVLTQLQWCFGKNDSYIGS--------RESP-HP 210

Query: 223 GIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQ-----------------HSIERVYISLT 282
             VHR+ +GTSG+L+ AK + A   L+A F +                   + ++Y +L 
Sbjct: 211 VPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALA 270

Query: 283 CGVPPSTAGRIEVPIG--RDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLV 342
            G+       I+ PIG  R P     +   S        K A S+  V+E   +   SLV
Sbjct: 271 DGIVEEDEVVIKQPIGVVRYPGVAQGLYVAS-----PEGKPAFSKVFVLERDREKNCSLV 330

Query: 343 EWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 358
           +  +++GR HQIR H  YMG PL+   ++ G++ +
Sbjct: 331 KVEIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECL 331

[Close]

BLAST of Carg03973-RA vs. TAIR10
Match: AT5G51140.2 (Pseudouridine synthase family protein)

HSP 1 Score: 66.6 bits (161), Expect = 4.5e-11
Identity = 69/284 (24.30%), Postives = 117/284 (41.20%), Query Frame = 0


Query: 73  SGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQP 132
           +GK  +D +      G  R     ++++G + V+G ++   S+ VK+  KI   +     
Sbjct: 72  TGKTIVDLFAD-EFKGRPRDYYVGAVKSGRIKVDGEIV-PVSYIVKSSQKITHFLH---- 131

Query: 133 LRAEPEDIYLDIV--YEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTN 192
            R EP  +  D+V  +++  V+ V KPA + VHP       T+V                
Sbjct: 132 -RHEPPVMIDDVVILHQEPDVVTVCKPASVPVHPCGQYRKNTIVG--------------- 191

Query: 193 EELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQ 252
                             IL +      + P  +HR+D+  SGLL++A+          Q
Sbjct: 192 ------------------ILDAEHDLGTLFP--IHRLDRLVSGLLIIARTAAKADFFRQQ 251

Query: 253 FKQHSIERVYISLTCGVPPSTAGRIEVPI---GRDPNNRIRMSAVSRTNNNSNAKYAASR 312
            +   +++ YI+   GV P     ++  I   G +  +    +  S  +     K A ++
Sbjct: 252 IEGGMVKKRYIAKVIGVFPEDEMIVDANINYNGSEGRSTAEDANSSGDDKKVKGKPACTK 311

Query: 313 YKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 352
           +  I+    G  SLV     TGRTHQIR H +Y G P+  D +Y
Sbjct: 312 FTRID--TNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLY 311

[Close]

BLAST of Carg03973-RA vs. TAIR10
Match: AT1G56345.1 (Pseudouridine synthase family protein)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-09
Identity = 74/302 (24.50%), Postives = 108/302 (35.76%), Query Frame = 0


Query: 143 DIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSDMDISDD 202
           DI+YEDE+++ VNKP             G    A+L      ++ +++E  L+       
Sbjct: 76  DILYEDEYLMAVNKP------------KGVYCEAVLRSAPQIVLDSSSEYHLA------- 135

Query: 203 ELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYIS 262
                                 +R+D+ TSG++++ K     A L   F +H I + YI+
Sbjct: 136 ----------------------NRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIA 195

Query: 263 LTCGVPPS------------------------TAGRIEVPIG---RDPNNRIRMSAVSRT 322
           L  G  P+                          GR+ +P G   RD      + +V+  
Sbjct: 196 LCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRV-LPGGSFVRDMETTFEVVSVNSV 255

Query: 323 NNNSNAKYAASRYKVIE---NLAQGGFSLVEWRL-----ETGRTHQIRAHAKYMGIPLLG 382
            N S      +   V E    L+ G               +GRTHQIR H +Y+GIP+ G
Sbjct: 256 KNESCELEDVNHVIVAEGERELSCGXXXXXXXXXXRAFPRSGRTHQIRLHCQYLGIPIRG 315

Query: 383 DEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCP 410
           D  Y G           RT               E   LHA  L   HP TG +I    P
Sbjct: 316 DVKYHGVYEW-----NGRT--------------FEGHELHAECLSLDHPVTGDSIVIRAP 316

[Close]

BLAST of Carg03973-RA vs. Swiss-Prot
Match: sp|Q3ECD0|PUS2_ARATH (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 2.9e-140
Identity = 246/374 (65.78%), Postives = 293/374 (78.34%), Query Frame = 0


Query: 48  CGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNG 107
           C    +  PR N AG+R+EEIV+   GK+RLDSWIS RI+G+SRARVQSSIR GLV+VNG
Sbjct: 54  CSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNG 113

Query: 108 RVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPG 167
           RV+DK SHNVK+GD++NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPG
Sbjct: 114 RVVDKVSHNVKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPG 173

Query: 168 NATGTLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRI 227
           N TGTLVN IL+HCSLP V  +N                          A +RPGIVHR+
Sbjct: 174 NPTGTLVNGILHHCSLPCVDYSNXXXXXXXXXXXXXXXXXXXXXXXXYAASVRPGIVHRL 233

Query: 228 DKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRI 287
           DKGT+GLLVVAKDEH+HAHL+ QFK H+IERVY+SLT GVP    GRIE+PIGRD +NRI
Sbjct: 234 DKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRI 293

Query: 288 RMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLG 347
           RM+A+        A++AASRYKVIE  A GG +LVEWRLETGRTHQIRAHAKYMG+PLLG
Sbjct: 294 RMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLG 353

Query: 348 DEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCP 407
           DEVYGGTKSMA+SLL+ R S S   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCP
Sbjct: 354 DEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCP 413

Query: 408 PPTDFAEILGQLRQ 422
           PP+D AEI+G LR+
Sbjct: 414 PPSDLAEIVGLLRR 427

[Close]

BLAST of Carg03973-RA vs. Swiss-Prot
Match: sp|Q5Z8P2|PUS2_ORYSJ (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.4e-134
Identity = 246/401 (61.35%), Postives = 296/401 (73.82%), Query Frame = 0


Query: 34  IATNHSVRCSGSDECGDETLPEPRTNYAGMRLEEIVETKSGKVRLDSWISCRI--DGISR 93
           +  +H+ RC  +D   +   P  R  + G RLEE V    G+ R+D+WIS R+   G+SR
Sbjct: 31  VGASHA-RCLAADANAEAVAPSRRGGHGGTRLEEAVPAGEGRSRIDAWISARLGGGGVSR 90

Query: 94  ARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHV 153
           AR+Q+SIRAGLV VNGR + K SH VK GD ++CT+ ELQPLRAEPEDI LDIVYED+H+
Sbjct: 91  ARIQASIRAGLVVVNGRPVSKVSHMVKGGDIVSCTVLELQPLRAEPEDIPLDIVYEDDHL 150

Query: 154 LVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSD--MDISDDELNSIEI 213
           LVVNKPAHMVVHPAPGNA GTLVNAIL+HC +   T      + D   D SDD+++  +I
Sbjct: 151 LVVNKPAHMVVHPAPGNANGTLVNAILHHCKISTFTCLARNSIDDECPDSSDDDIDVFDI 210

Query: 214 -------LSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSIERVYIS 273
                  +SS V EA +RPGIVHR+DKGTSGLLVVAKDEH+HA L+ QFK H+I RVYIS
Sbjct: 211 DQFTTGEVSSEVREALVRPGIVHRLDKGTSGLLVVAKDEHSHAQLAEQFKLHTIRRVYIS 270

Query: 274 LTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQGGFSLV 333
           LTCG P   +GRIEVPI RDPNNRIRM A   + +   A++AASRYKV E  A GG +LV
Sbjct: 271 LTCGAPNPNSGRIEVPIARDPNNRIRMIATPGSGHR-YARHAASRYKVREVFAGGGSALV 330

Query: 334 EWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQLVSSLE 393
           EWRLETGRTHQIRAHAKY+GIPLLGDE YGGTKSMA+SLLR RT S  H  L  ++S ++
Sbjct: 331 EWRLETGRTHQIRAHAKYLGIPLLGDETYGGTKSMALSLLRPRTPSRYHCDLSNMISKID 390

Query: 394 RPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQIS 424
           RPCLHA  LGF HPH+GK + FSCPPP DF E+L +L Q++
Sbjct: 391 RPCLHAALLGFKHPHSGKILEFSCPPPDDFTEVLNELHQVT 429

[Close]

BLAST of Carg03973-RA vs. Swiss-Prot
Match: sp|P50513|RLUD_ZYMMO (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 1.1e-54
Identity = 143/350 (40.86%), Postives = 194/350 (55.43%), Query Frame = 0


Query: 77  RLDSWISCRIDGISRARVQSSIRAGLVS-VNGRVIDKASHNVKAGDKINCTISELQPLRA 136
           RLD  ++  I  +SR R+++ I +G VS   G ++   +  +K+ D     I   +P   
Sbjct: 16  RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75

Query: 137 EPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLS 196
           EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN   TLVNA+L HC+             
Sbjct: 76  EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA------------- 135

Query: 197 DMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHS 256
                  +L+ I  ++        RPGIVHRIDK TSGLLVVAK + AHA L+AQF  HS
Sbjct: 136 ------GKLSGIGGVA--------RPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHS 195

Query: 257 IERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLA 316
           I R Y ++  G  PS  G ++ P+ R   NR +M+ VS    +   K A + Y++I  L 
Sbjct: 196 INRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIVS----DGRGKRAVTHYRMITPLK 255

Query: 317 QGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLM 376
               SL+E RLETGRTHQ+R H   +G  LLGD VYG +K               H  L+
Sbjct: 256 NA--SLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSK-------------KAHHALL 315

Query: 377 QLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQISTK 426
           Q + + +R  LHA  LGFIHP +GK + F    P D  ++L ++  IS +
Sbjct: 316 QSL-AFQRQALHAAHLGFIHPISGKQVDFDAEMPQDM-QLLFKMLMISNR 316

[Close]

BLAST of Carg03973-RA vs. Swiss-Prot
Match: sp|Q9CKA6|RLUD_PASMU (Ribosomal large subunit pseudouridine synthase D OS=Pasteurella multocida (strain Pm70) OX=272843 GN=rluD PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.4e-48
Identity = 118/344 (34.30%), Postives = 177/344 (51.45%), Query Frame = 0


Query: 77  RLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAE 136
           RLD  ++      SR+R+++ I   LV VNG V++     V  G++I   +      R E
Sbjct: 18  RLDQALAELFPDYSRSRLKTWIEQNLVHVNGDVVNVPRAKVYGGEQIEIVVEIDDDTRFE 77

Query: 137 PEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSD 196
           PE++ L+IVYED+ +LV+NKP   VVHP  GNA+GT++NA+L H                
Sbjct: 78  PENLPLNIVYEDDDILVINKPKDFVVHPGAGNASGTVLNALLYH---------------- 137

Query: 197 MDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSI 256
                            ++E P R GIVHR+DK T+GL+V+AK   A   L    ++  I
Sbjct: 138 --------------YPAIAEVP-RAGIVHRLDKDTTGLMVIAKTIPAQTKLVRDLQKRKI 197

Query: 257 ERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQ 316
            R Y ++ CG+  +  G ++ P+ R P  R  M+       +   K A + Y+++E    
Sbjct: 198 TREYEAIACGI-MTKGGMVDQPMARHPTKRTHMAV------HPMGKPAVTHYRIMERFR- 257

Query: 317 GGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQ 376
             ++ +  RLETGRTHQIR H  ++  PLLGD+ YGG         R R   +   +LMQ
Sbjct: 258 -NYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQTYGG---------RPRPPKNASEELMQ 312

Query: 377 LVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLR 421
            +   +R  LHA+ L   HP TG+ + +  P PTDF E++  L+
Sbjct: 318 TLRDFKRQALHAIMLRLAHPITGELMEWHAPLPTDFVELVEALK 312

[Close]

BLAST of Carg03973-RA vs. Swiss-Prot
Match: sp|Q45480|YLYB_BACSU (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)

HSP 1 Score: 193.7 bits (491), Expect = 4.4e-48
Identity = 126/345 (36.52%), Postives = 176/345 (51.01%), Query Frame = 0


Query: 77  RLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAGDKINCTISELQPLRAE 136
           R+D +++   +  SR +VQ  ++ G V VNG  + KA++ ++ GD++  T+ E + L   
Sbjct: 16  RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75

Query: 137 PEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVTTTNEELLSD 196
                          LVVNKP  MVVHPAPG+ TGTLVN ++ HC+              
Sbjct: 76  AXXXXXXXXXXXXXXLVVNKPRGMVVHPAPGHLTGTLVNGLMAHCT-------------- 135

Query: 197 MDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAKDEHAHAHLSAQFKQHSI 256
            D+S   +N +           +RPGIVHRIDK TSGLL+VAK++ AH  L  Q    ++
Sbjct: 136 -DLSG--INGV-----------MRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTV 195

Query: 257 ERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNSNAKYAASRYKVIENLAQ 316
            R Y ++  G+     G I+ PIGRD  +R  M+ V+R   N     A + + V+E    
Sbjct: 196 TRKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMT-VTRDGKN-----AVTHFHVLERFQD 255

Query: 317 GGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRTSSSCHSQLMQ 376
             F+LVE +LETGRTHQIR H KY+G PL GD  YG  K++                   
Sbjct: 256 --FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI------------------- 301

Query: 377 LVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQLRQ 422
                    LHA  LGF HP TG+ + F  P P D AE++  LR+
Sbjct: 316 ---DFNGQALHAGVLGFDHPRTGEYVEFEAPLPEDMAELIENLRK 301

[Close]

BLAST of Carg03973-RA vs. TrEMBL
Match: tr|A0A1S3BCE4|A0A1S3BCE4_CUCME (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 2.0e-194
Identity = 354/427 (82.90%), Postives = 375/427 (87.82%), Query Frame = 0


Query: 1   MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
           MIS+CSAR IQA+P SI  FFPRNR RI  +VLR + N S RCS  DE   E   E   N
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60

Query: 61  YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
           YAG RLEE V+   GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
           HCSLPMV+T+  E  SD D+SDDE +SIE+  SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
           DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A   T NN 
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300

Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           +AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360

Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
           SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420

Query: 421 RQISTKK 427
           R+I TKK
Sbjct: 421 REIGTKK 425

[Close]

BLAST of Carg03973-RA vs. TrEMBL
Match: tr|A0A1S3BD56|A0A1S3BD56_CUCME (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 2.0e-194
Identity = 354/427 (82.90%), Postives = 375/427 (87.82%), Query Frame = 0


Query: 1   MISICSARAIQAAP-SIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
           MIS+CSAR IQA+P SI  FFPRNR RI  +VLR + N S RCS  DE   E   E   N
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCSDYDEY-SEAQSERIAN 60

Query: 61  YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
           YAG RLEE V+   GKVRLDSWIS RI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
           HCSLPMV+T+  E  SD D+SDDE +SIE+  SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
           DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A   T NN 
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNP 300

Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           +AK+AASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360

Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
           SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDFAEIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQL 420

Query: 421 RQISTKK 427
           R+I TKK
Sbjct: 421 REIGTKK 425

[Close]

BLAST of Carg03973-RA vs. TrEMBL
Match: tr|A0A0A0LKD7|A0A0A0LKD7_CUCSA (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 2.6e-194
Identity = 351/427 (82.20%), Postives = 374/427 (87.59%), Query Frame = 0


Query: 1   MISICSARAIQAAPS-IQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETLPEPRTN 60
           MIS+CSAR IQA+PS I  FFP NR RI  +VLRI++N S RCS   +   E   E   N
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKA 120
           YAG RLEE V+   GKVRLDSWISCRI+GISRARVQSSIRAGLVSVNGRVIDK SHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD INCTISELQPLRAEPEDI LDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGTSGLLVVAK 240
           HCSLPMV T+  E  SD D+SDDE +SI +  SRVSEAPIRPGIVHR+DKGTSGLLVVAK
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVF-SRVSEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAVSRTNNNS 300
           DEH+HAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI RDPNNRIRM+A+  T NN 
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNP 300

Query: 301 NAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           +AKYAASRY V+E LAQGG SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM
Sbjct: 301 HAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360

Query: 361 SLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDFAEILGQL 420
           SLLRTR SS+CH QLMQLVSSLERPCLHALTLGF+HPHTGKNIRFSCPPPTDF EIL QL
Sbjct: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQL 420

Query: 421 RQISTKK 427
           R+I T+K
Sbjct: 421 REIGTQK 426

[Close]

BLAST of Carg03973-RA vs. TrEMBL
Match: tr|A0A2I4DLV5|A0A2I4DLV5_9ROSI (RNA pseudouridine synthase 2, chloroplastic isoform X2 OS=Juglans regia OX=51240 GN=LOC108981429 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.4e-155
Identity = 297/437 (67.96%), Postives = 341/437 (78.03%), Query Frame = 0


Query: 3   SICSARAIQAAPSIQLFFPRNRGRISYDVLRI--ATNHSVRCSGSDECGDETLPEPRTNY 62
           S+  +   Q++P    F PRN  R     LRI    + S   + SDE   E L   R N+
Sbjct: 10  SLAKSFIFQSSPPFFCFSPRN-PRTPSSSLRICFVASSSSGSTNSDE-DKEDLAGRRANF 69

Query: 63  AGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVIDKASHNVKAG 122
            G++LE+ V   SGK+RLDSWIS RI GISRARVQSSIR+GLV+VNGRV+DK SHNVK G
Sbjct: 70  GGVQLEDTVSLNSGKLRLDSWISSRIGGISRARVQSSIRSGLVAVNGRVVDKVSHNVKTG 129

Query: 123 DKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNH 182
           DK+NCTISELQPLRA PEDI LDIV+EDEHVLVVNKPAHMVVHPAPGNATGTLVN +L+H
Sbjct: 130 DKVNCTISELQPLRAVPEDIPLDIVFEDEHVLVVNKPAHMVVHPAPGNATGTLVNGVLHH 189

Query: 183 CSLPMVTTTNEELLSDM-DISDDELNSIEI-------LSSRVSEAPIRPGIVHRIDKGTS 242
           CS P+V  +NEE+LSD  DISDDEL+S          L SR S A IRPGIVHR+DKGTS
Sbjct: 190 CSPPVVAFSNEEVLSDAEDISDDELSSFSAGQNPNDGLYSRRSGASIRPGIVHRLDKGTS 249

Query: 243 GLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSAV 302
           GLLVVAKDEH+HA+LS QFK H+I+R+Y+SLT GVP   AGR+EVPIGRD NNRIRM+A+
Sbjct: 250 GLLVVAKDEHSHANLSEQFKLHTIQRLYVSLTSGVPSPMAGRVEVPIGRDLNNRIRMAAI 309

Query: 303 SRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYG 362
             +N +  A++AASRYKVIE LA G  +LVEWRLETGRTHQIRAHAKY+GIPLLGDEVYG
Sbjct: 310 PGSNISRQARHAASRYKVIEILAGGSSALVEWRLETGRTHQIRAHAKYLGIPLLGDEVYG 369

Query: 363 GTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTDF 422
           GTKSMA+SLLR RT  S H QL+QLVS LERPCLHAL LGF+HPHT +N+ F CPPP DF
Sbjct: 370 GTKSMALSLLRPRTPPSRHGQLLQLVSRLERPCLHALALGFMHPHTMENVYFQCPPPADF 429

Query: 423 AEILGQLRQISTKKGMF 430
           AEILGQLR+I T K  F
Sbjct: 430 AEILGQLRKIGTVKHSF 444

[Close]

BLAST of Carg03973-RA vs. TrEMBL
Match: tr|A0A2N9FME9|A0A2N9FME9_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15953 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 3.7e-153
Identity = 293/435 (67.36%), Postives = 339/435 (77.93%), Query Frame = 0


Query: 1   MISIC-------SARAIQAAPSIQLFFPRNRGRISYDVLRIATNHSVRCSGSDECGDETL 60
           M+S+C       S++  Q +PS+   F     R    +LR  T      S S+E G E+L
Sbjct: 505 MLSLCYSSCSSSSSKLFQTSPSLFFPFSPRNPRFPSRLLRNFT-----ASSSNE-GQESL 564

Query: 61  PEP--RTNYAGMRLEEIVETKSGKVRLDSWISCRIDGISRARVQSSIRAGLVSVNGRVID 120
                R NY G+ LEE V+ KSGK+RLDSWIS RI GISRARVQSSIR+GLVSVNGR++D
Sbjct: 565 AVAVRRANYGGVTLEETVDIKSGKLRLDSWISSRISGISRARVQSSIRSGLVSVNGRIVD 624

Query: 121 KASHNVKAGDKINCTISELQPLRAEPEDIYLDIVYEDEHVLVVNKPAHMVVHPAPGNATG 180
           K SHNVKAGDK+NCTISELQPL+AE E+I L+IVYEDEHVLVVNKPAHMVVHPAPGNA+G
Sbjct: 625 KVSHNVKAGDKVNCTISELQPLKAEAENIALNIVYEDEHVLVVNKPAHMVVHPAPGNASG 684

Query: 181 TLVNAILNHCSLPMVTTTNEELLSDMDISDDELNSIEILSSRVSEAPIRPGIVHRIDKGT 240
           TLVN IL+HCSLP    +NEE+LSD + S D+       S  +S A IRPGIVHR+DKGT
Sbjct: 685 TLVNGILHHCSLPF---SNEEILSDDEFSTDQ-------SPNISAASIRPGIVHRLDKGT 744

Query: 241 SGLLVVAKDEHAHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPIGRDPNNRIRMSA 300
           SGLLVVAKDEH+HAHLS QFK H+I+RVYISLT G+P   AGR+EVPIGRD NNRIRM+A
Sbjct: 745 SGLLVVAKDEHSHAHLSEQFKLHTIQRVYISLTSGMPSPVAGRVEVPIGRDLNNRIRMAA 804

Query: 301 VSRTNNNSNAKYAASRYKVIENLAQGGFSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 360
           +  +NN    ++AASRYKVIE LA GG +LVEWRLETGRTHQIRAHAKY+GIPLLGDEVY
Sbjct: 805 IPGSNNCRQTRHAASRYKVIEILAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDEVY 864

Query: 361 GGTKSMAMSLLRTRTSSSCHSQLMQLVSSLERPCLHALTLGFIHPHTGKNIRFSCPPPTD 420
           GGTKSMA+SLLR RT+ S H QL+QLVS LERPCLHAL LGF HPHT +N+ FS PPP D
Sbjct: 865 GGTKSMALSLLRPRTAPSHHGQLLQLVSGLERPCLHALALGFKHPHTMENVCFSSPPPAD 923

Query: 421 FAEILGQLRQISTKK 427
           FAEIL QLR+I T+K
Sbjct: 925 FAEILSQLREIGTEK 923

The following BLAST results are available for this feature:

BLAST of Carg03973-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951936.1	1.4e-241	99.77	RNA pseudouridine synthase 2, chloroplastic [Cucurbita moschata]	[more]
XP_023538538.1	1.8e-239	98.59	RNA pseudouridine synthase 2, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_023002352.1	2.9e-234	97.42	RNA pseudouridine synthase 2, chloroplastic [Cucurbita maxima]	[more]
XP_022142903.1	8.0e-208	87.56	RNA pseudouridine synthase 2, chloroplastic isoform X1 [Momordica charantia] >XP...	[more]
XP_008445318.1	3.0e-194	82.90	PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]...	[more]

BLAST of Carg03973-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76050.2	1.6e-141	65.78	Pseudouridine synthase family protein	[more]
AT3G19440.1	4.3e-14	27.88	Pseudouridine synthase family protein	[more]
AT3G52260.3	1.5e-11	25.45	Pseudouridine synthase family protein	[more]
AT5G51140.2	4.5e-11	24.30	Pseudouridine synthase family protein	[more]
AT1G56345.1	1.4e-09	24.50	Pseudouridine synthase family protein	[more]

BLAST of Carg03973-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q3ECD0\|PUS2_ARATH	2.9e-140	65.78	RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
sp\|Q5Z8P2\|PUS2_ORYSJ	1.4e-134	61.35	RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3...	[more]
sp\|P50513\|RLUD_ZYMMO	1.1e-54	40.86	Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob...	[more]
sp\|Q9CKA6\|RLUD_PASMU	4.4e-48	34.30	Ribosomal large subunit pseudouridine synthase D OS=Pasteurella multocida (strai...	[more]
sp\|Q45480\|YLYB_BACSU	4.4e-48	36.52	Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168...	[more]

BLAST of Carg03973-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BCE4\|A0A1S3BCE4_CUCME	2.0e-194	82.90	Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1	[more]
tr\|A0A1S3BD56\|A0A1S3BD56_CUCME	2.0e-194	82.90	Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1	[more]
tr\|A0A0A0LKD7\|A0A0A0LKD7_CUCSA	2.6e-194	82.20	Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1	[more]
tr\|A0A2I4DLV5\|A0A2I4DLV5_9ROSI	1.4e-155	67.96	RNA pseudouridine synthase 2, chloroplastic isoform X2 OS=Juglans regia OX=51240...	[more]
tr\|A0A2N9FME9\|A0A2N9FME9_FAGSY	3.7e-153	67.36	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15953 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0009982	pseudouridine synthase activity
GO:0003723	RNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0009451	RNA modification
GO:0001522	pseudouridine synthesis

Vocabulary: **INTERPRO**
Term	Definition
IPR020103	PsdUridine_synth_cat_dom_sf
IPR006224	PsdUridine_synth_RluC/D_CS
IPR006145	PsdUridine_synth_RsuA/RluD
IPR006225	PsdUridine_synth_RluC/D
IPR036986	S4_RNA-bd_sf
IPR002942	S4_RNA-bd

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg03973	Carg03973	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03973-RA.three_prime_UTR.1	Carg03973-RA.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03973-RA.CDS.8	Carg03973-RA.CDS.8	CDS
Carg03973-RA.CDS.7	Carg03973-RA.CDS.7	CDS
Carg03973-RA.CDS.6	Carg03973-RA.CDS.6	CDS
Carg03973-RA.CDS.5	Carg03973-RA.CDS.5	CDS
Carg03973-RA.CDS.4	Carg03973-RA.CDS.4	CDS
Carg03973-RA.CDS.3	Carg03973-RA.CDS.3	CDS
Carg03973-RA.CDS.2	Carg03973-RA.CDS.2	CDS
Carg03973-RA.CDS.1	Carg03973-RA.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03973-RA.five_prime_UTR.1	Carg03973-RA.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03973-RA.exon.8	Carg03973-RA.exon.8	exon
Carg03973-RA.exon.7	Carg03973-RA.exon.7	exon
Carg03973-RA.exon.6	Carg03973-RA.exon.6	exon
Carg03973-RA.exon.5	Carg03973-RA.exon.5	exon
Carg03973-RA.exon.4	Carg03973-RA.exon.4	exon
Carg03973-RA.exon.3	Carg03973-RA.exon.3	exon
Carg03973-RA.exon.2	Carg03973-RA.exon.2	exon
Carg03973-RA.exon.1	Carg03973-RA.exon.1	exon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg03973-RA	Carg03973-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002942	RNA-binding S4 domain	SMART	SM00363	s4_6	coord: 76..138 e-value: 0.0013 score: 28.0
IPR002942	RNA-binding S4 domain	PFAM	PF01479	S4	coord: 77..123 e-value: 7.7E-8 score: 31.8
IPR002942	RNA-binding S4 domain	PROSITE	PS50889	S4	coord: 76..149 score: 12.041
IPR002942	RNA-binding S4 domain	CDD	cd00165	S4	coord: 76..155 e-value: 9.24812E-12 score: 59.9558
IPR036986	RNA-binding S4 domain superfamily	GENE3D	G3DSA:3.10.290.10		coord: 60..131 e-value: 2.6E-16 score: 60.9
IPR006225	Pseudouridine synthase, RluC/RluD	TIGRFAM	TIGR00005	TIGR00005	coord: 75..419 e-value: 5.7E-75 score: 250.3
IPR006145	Pseudouridine synthase, RsuA/RluB/C/D/E/F	PFAM	PF00849	PseudoU_synth_2	coord: 150..338 e-value: 3.2E-33 score: 115.0
IPR006145	Pseudouridine synthase, RsuA/RluB/C/D/E/F	CDD	cd02869	PseudoU_synth_RluCD_like	coord: 151..393 e-value: 8.43858E-65 score: 208.346
None	No IPR available	PANTHER	PTHR44168:SF1	RNA PSEUDOURIDINE SYNTHASE 2, CHLOROPLASTIC	coord: 55..423
None	No IPR available	PANTHER	PTHR44168	FAMILY NOT NAMED	coord: 55..423
None	No IPR available	SUPERFAMILY	SSF55174	Alpha-L RNA-binding motif	coord: 75..163
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 224..238
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	SSF55120	Pseudouridine synthase	coord: 136..180 coord: 214..420