CSPI07G19840 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI07G19840
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRING/U-box superfamily protein
LocationChr7 : 17363975 .. 17364845 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGAAATCAATACGTGTTTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCTACGAAGATCGAAAATGGATATTCCTCCGTTTCTTGTCTTGCTTTGAACTGTCCTTCCATTATAGATTTTCACTCTTGCTGGTAACTATAAGTAAATTTAGGGTTTAAGAAAGTTGAAGGGTTGGCGTAGAAATGACATATGGATCAATTACTTTACTTTGCAGGAGAATGCTACCGAAGGAAATAGGTGAGAAATGGAACAAAGCACTTTGCGAAGTGCTTTATTCGACAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCCGTGGGAATAACCCTTGAGAGGGGCGCAAGCATAAGGGACTGTGAGTGTCCGCTTTGCCATAGACTCTTTTGCGCGACATGTGAAGTCCCATGGCACGATGGTGTCACCTGCGAGGAATATAAGAGACTGTATAAAAATGAAGAAGGTAGAAATGAGATTTTGATGAAGAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCAATTTCTTCGTTGAGAAGATTTCTGGTTGTTTGCATATCACTTGCAGGTTCATTAATTTCTAATTCCCTCTTTCTTCTATTTTTATACACAACAGTCTAGACTATGTATCAGGACTTTCGATCAGTATTTGAGCCATATGAAGTGGAGGGATGAGTAATGTTAATAATTTATTTTGTATATATATATATATATAAATCATGATTACATATTAACATTCTAATTTGTTTATATATATTGTTTATGAAATCGTTGTAGGTGTCGATTCGAGTTCTGCTATGCTTGTGGATCGGCTTGGTCTTCCACCCATGGTGGTTGCCAACCTAACTACTGA

mRNA sequence

ATGTTCGAAATCAATACGTGTTTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCTACGAAGATCGAAAATGGATATTCCTCCGTTTCTTGTCTTGCTTTGAACTGTCCTTCCATTATAGATTTTCACTCTTGCTGGAGAATGCTACCGAAGGAAATAGGTGAGAAATGGAACAAAGCACTTTGCGAAGTGCTTTATTCGACAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCCGTGGGAATAACCCTTGAGAGGGGCGCAAGCATAAGGGACTGTGAGTGTCCGCTTTGCCATAGACTCTTTTGCGCGACATGTGAAGTCCCATGGCACGATGGTGTCACCTGCGAGGAATATAAGAGACTGTATAAAAATGAAGAAGGTAGAAATGAGATTTTGATGAAGAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCAATTTCTTCGTTGAGAAGATTTCTGGTTGTTTGCATATCACTTGCAGGTGTCGATTCGAGTTCTGCTATGCTTGTGGATCGGCTTGGTCTTCCACCCATGGTGGTTGCCAACCTAACTACTGA

Coding sequence (CDS)

ATGTTCGAAATCAATACGTGTTTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCTACGAAGATCGAAAATGGATATTCCTCCGTTTCTTGTCTTGCTTTGAACTGTCCTTCCATTATAGATTTTCACTCTTGCTGGAGAATGCTACCGAAGGAAATAGGTGAGAAATGGAACAAAGCACTTTGCGAAGTGCTTTATTCGACAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCCGTGGGAATAACCCTTGAGAGGGGCGCAAGCATAAGGGACTGTGAGTGTCCGCTTTGCCATAGACTCTTTTGCGCGACATGTGAAGTCCCATGGCACGATGGTGTCACCTGCGAGGAATATAAGAGACTGTATAAAAATGAAGAAGGTAGAAATGAGATTTTGATGAAGAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCAATTTCTTCGTTGAGAAGATTTCTGGTTGTTTGCATATCACTTGCAGGTGTCGATTCGAGTTCTGCTATGCTTGTGGATCGGCTTGGTCTTCCACCCATGGTGGTTGCCAACCTAACTACTGA
Homology
[Close]
BLAST of CSPI07G19840 vs. Swiss-Prot
Match: RN217_XENLA (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis GN=rnf217 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-17
Identity = 53/186 (28.49%), Postives = 83/186 (44.62%), Query Frame = 1 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C    C +C+ +++S++++ G + + C    C   +D  +    LP +   K+ K   E+
Sbjct: 18  CKKPVCDECLKRYLSSQVQLGQAEIQCPITECNKHLDESTILYSLPHDDIIKY-KYFLEL 77

Query: 67  LYSTEEKVVCPFKDCSVGITLERGASIRD---------CECPLCHRLFCATCEVPWHDGV 126
                    CP   C    T +R   I +          +CP C  ++C  C  PWH+GV
Sbjct: 78  SRMDSSTKPCP--QCKHFTTFKRKTHIPNPTKSENKLKIQCPSCQFIWCFRCHAPWHEGV 137

Query: 127 TCEEYKRLYK-NEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITC-RCRFEFCY 182
            C EYK+  K      NEI       Q    KCP+C   +++  GC H+TC +C   FCY
Sbjct: 138 NCREYKKGDKLLRHWANEI----EHGQRNAQKCPRCKVHIQRTEGCDHMTCSQCNTNFCY 196

[Close]
BLAST of CSPI07G19840 vs. Swiss-Prot
Match: RN217_MOUSE (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 2.8e-17
Identity = 52/188 (27.66%), Postives = 85/188 (45.21%), Query Frame = 1 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C  + C +C+  ++S++++ G   + C    C   ++  +    L  E   K+ K   E+
Sbjct: 251 CKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLEETTVVYNLTHEDSIKY-KYFLEL 310

Query: 67  LYSTEEKVVCP-------FKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTC 126
                    CP       FK      T  R  S    +CP C  ++C  C  PWH+GV C
Sbjct: 311 GRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYKIQCPTCQLIWCFKCHSPWHEGVNC 370

Query: 127 EEYKRLYKNEEGRNEILMKKLASQMKW-----MKCPKCNFFVEKISGCLHITC-RCRFEF 182
           +EYK        + + L++  AS+++       KCPKC   +++  GC H+TC +C   F
Sbjct: 371 KEYK--------KGDKLLRHWASEIEHGQRNAQKCPKCKIHIQRTEGCDHMTCSQCNTNF 429

[Close]
BLAST of CSPI07G19840 vs. Swiss-Prot
Match: ARI5_ARATH (Probable E3 ubiquitin-protein ligase ARI5 OS=Arabidopsis thaliana GN=ARI5 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.3e-17
Identity = 58/186 (31.18%), Postives = 82/186 (44.09%), Query Frame = 1 

Query: 6   TCLHSFCRDCVIKHVSTKIENGYS--SVSCLALNCPSIIDFHSCWRMLPKEIGEKWNK-A 65
           +C H FC  C   ++ST I +G     + C   +CP+ I      ++  KE  EK+ +  
Sbjct: 148 SCGHPFCATCWTGYISTTINDGPGCLMLKCPDPSCPAAIGRDMIDKLASKEDKEKYYRYF 207

Query: 66  LCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCEEY 125
           L   +    E   CP   C   I    G    D  C LC   FC  C    H  V C+  
Sbjct: 208 LRSYVEVNREMKWCPAPGCEHAIDFAGGTESYDVSC-LCSHSFCWNCTEEAHRPVDCDTV 267

Query: 126 KRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR--CRFEFCYACGSA 185
            +        +E +   LA+      CPKC   +EK  GC+H+TC   C+FEFC+ C +A
Sbjct: 268 GKWILKNSAESENMNWILANS---KPCPKCKRPIEKNHGCMHMTCTPPCKFEFCWLCLNA 327

Query: 186 WSSTHG 187
           W+  HG
Sbjct: 328 WTE-HG 328

[Close]
BLAST of CSPI07G19840 vs. Swiss-Prot
Match: RN217_HUMAN (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens GN=RNF217 PE=2 SV=4)

HSP 1 Score: 89.0 bits (219), Expect = 6.3e-17
Identity = 52/188 (27.66%), Postives = 84/188 (44.68%), Query Frame = 1 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C  + C +C+  ++S +++ G   + C    C   ++  +    L  E   K+ K   E+
Sbjct: 278 CKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLEETTVVYNLTHEDSIKY-KYFLEL 337

Query: 67  LYSTEEKVVCP-------FKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTC 126
                    CP       FK      T  R  S    +CP C  ++C  C  PWH+GV C
Sbjct: 338 GRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYKIQCPTCQFVWCFKCHSPWHEGVNC 397

Query: 127 EEYKRLYKNEEGRNEILMKKLASQMKW-----MKCPKCNFFVEKISGCLHITC-RCRFEF 182
           +EYK        + + L++  AS+++       KCPKC   +++  GC H+TC +C   F
Sbjct: 398 KEYK--------KGDKLLRHWASEIEHGQRNAQKCPKCKIHIQRTEGCDHMTCSQCNTNF 456

[Close]
BLAST of CSPI07G19840 vs. Swiss-Prot
Match: ARI7_ARATH (Probable E3 ubiquitin-protein ligase ARI7 OS=Arabidopsis thaliana GN=ARI7 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.1e-16
Identity = 55/189 (29.10%), Postives = 80/189 (42.33%), Query Frame = 1 

Query: 6   TCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCP-----SIIDFHSCWRMLPKEIGEKWN 65
           +C H FC  C   ++ST I +G     CL L CP     + +      ++  ++  EK+N
Sbjct: 153 SCGHPFCTTCWTGYISTTINDG---PGCLMLRCPDPSCLAAVGHDMVDKLASEDEKEKYN 212

Query: 66  KALCEVLYSTEEKVV-CPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTC 125
           +           K+  CP   C   I    G+   D  C LC   FC  C    H  V C
Sbjct: 213 RYFLRSYIEDNRKMKWCPAPGCDFAIDFVAGSGNYDVSC-LCSFSFCWNCTEEAHRPVDC 272

Query: 126 EEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR--CRFEFCYAC 185
               +        +E +   LA+      CP+C   +EK  GC+H+TC   C++EFC+ C
Sbjct: 273 STVSKWILKNSAESENMNWILANS---KPCPRCKRPIEKNQGCMHMTCTPPCKYEFCWLC 332

Query: 186 GSAWSSTHG 187
             AW   HG
Sbjct: 333 LGAWMD-HG 333

[Close]
BLAST of CSPI07G19840 vs. TrEMBL
Match: A0A0A0K9X9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430760 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 7.1e-100
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN
Sbjct: 17  MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 76

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 120
           KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE
Sbjct: 77  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 136

Query: 121 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR 169
           EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKI GCLHITCR
Sbjct: 137 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKIYGCLHITCR 184

[Close]
BLAST of CSPI07G19840 vs. TrEMBL
Match: A0A072VQ64_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_1g105460 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-63
Identity = 101/187 (54.01%), Postives = 138/187 (73.80%), Query Frame = 1 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C HSFC +CV+K V+TKI++  ++VSC  LNC  +++  SC ++LPKE+ +KWN ALCE 
Sbjct: 121 CYHSFCSECVVKQVATKIQDNITNVSCPGLNCKGVLELESCRKLLPKELIDKWNDALCEA 180

Query: 67  LYSTEEKVVCPFKDCSVGITLERGA--SIRDCECPLCHRLFCATCEVPWHDGVTCEEYKR 126
            + T  K  CPFKDCS  +  E      +R+ EC  CHRLFCA C VPWH G+ CEEY++
Sbjct: 181 FFLTVPKFYCPFKDCSAMLLDEDEGREDVRESECASCHRLFCAKCHVPWHPGIGCEEYQK 240

Query: 127 LYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSAWSST 186
           L  +E GR ++L+++LA++ KW +CP+C F+VEK  GCLHITCRC+FEFCY+CG+ W+S+
Sbjct: 241 LNVDERGREDLLVRELANKKKWKRCPRCRFYVEKRDGCLHITCRCKFEFCYSCGAQWTSS 300

Query: 187 HGGCQPN 192
           HGGCQ N
Sbjct: 301 HGGCQRN 307

[Close]
BLAST of CSPI07G19840 vs. TrEMBL
Match: A0A067LCT7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_26932 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.6e-59
Identity = 97/194 (50.00%), Postives = 136/194 (70.10%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSV-SCLALNCPSIIDFHSCWRMLPKEIGEKW 60
           MF+I++C+HSFC DC+ KHV+TKI++   ++ +C  +NCP+I++   C   L K + + W
Sbjct: 100 MFKIDSCVHSFCSDCISKHVATKIQDSACTIITCPGMNCPAILELDICRAQLSKGVIDIW 159

Query: 61  NKALCEVLYSTEEKVVCPFKDCSVGITLERGA--SIRDCECPLCHRLFCATCEVPWHDGV 120
            + LCE + S  EK  CPFKDCS  +  + G   +IR+ ECP CHRLFCA C VPWH GV
Sbjct: 160 EEILCEEMISESEKFYCPFKDCSALLVNDNGGGEAIRESECPFCHRLFCAQCYVPWHSGV 219

Query: 121 TCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYAC 180
            CE ++ L ++E GR ++++ +LA   KW +CP C FFVE+  GC H+TCRC+FEFCY C
Sbjct: 220 ECEVFQWLKEDERGREDLMVMELARDKKWSRCPHCKFFVERTEGCPHMTCRCKFEFCYGC 279

Query: 181 GSAWSSTHGGCQPN 192
           GS W+++HGGCQ N
Sbjct: 280 GSQWTASHGGCQRN 293

[Close]
BLAST of CSPI07G19840 vs. TrEMBL
Match: A0A059C8R9_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E03591 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.7e-59
Identity = 95/194 (48.97%), Postives = 134/194 (69.07%), Query Frame = 1 

Query: 1   MFEINT-CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKW 60
           MF I T C H+FC  C+ +HV TK+ +  +++ C  L C  +++  SC ++LP+ +   W
Sbjct: 120 MFRIRTACSHAFCSLCISRHVETKVLDNVTTIKCPGLECEGVLEIDSCRQILPENVLSVW 179

Query: 61  NKALCEVLYSTEEKVVCPFKDCSVGIT--LERGASIRDCECPLCHRLFCATCEVPWHDGV 120
            K+LCE  +S  EK  CPFK+CS  +    + G  IRDCECP CHRLFCA C VPWH GV
Sbjct: 180 EKSLCEATFSEMEKFYCPFKNCSALLIKDCDDGKVIRDCECPYCHRLFCAECSVPWHAGV 239

Query: 121 TCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYAC 180
           +CEE++ L ++E GR ++++++LA + KW +CPKC F+VE+  GC HITCRC+F+FCY C
Sbjct: 240 SCEEFQSLNEDERGREDLIVRELAKEKKWGRCPKCKFYVERTQGCPHITCRCQFQFCYGC 299

Query: 181 GSAWSSTHGGCQPN 192
           GS W+ +HGGC  N
Sbjct: 300 GSEWTQSHGGCARN 313

[Close]
BLAST of CSPI07G19840 vs. TrEMBL
Match: A0A068VLN7_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00003346001 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 3.5e-59
Identity = 91/188 (48.40%), Postives = 128/188 (68.09%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF I +C H FC +C+ KHV+ ++E     + C A+NC S I+F SC   +PK++ EKW+
Sbjct: 115 MFTIQSCGHVFCNECISKHVAARLEYNVHGIRCPAVNCGSAIEFDSCGSFMPKDVLEKWD 174

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 120
           + LC+ L    +K  CPFKDCS  +  +    IR+ ECP+C RLFCA C VPWH GV CE
Sbjct: 175 EMLCDALVDASQKFYCPFKDCSAMLVRDSDEVIRESECPICRRLFCAQCYVPWHPGVDCE 234

Query: 121 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSA 180
           E+ RL ++E GR ++++++LA    W +CP+C ++VE+  GC+H+TCRC F+FCY CG  
Sbjct: 235 EFHRLNEDERGREDLMLRELAKAKSWNRCPRCKYYVERNEGCIHMTCRCGFQFCYTCGEQ 294

Query: 181 WSSTHGGC 189
           WSS HGGC
Sbjct: 295 WSSAHGGC 302

[Close]
BLAST of CSPI07G19840 vs. TAIR10
Match: AT3G14250.1 (AT3G14250.1 RING/U-box superfamily protein)

HSP 1 Score: 194.5 bits (493), Expect = 6.0e-50
Identity = 79/188 (42.02%), Postives = 122/188 (64.89%), Query Frame = 1 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C H++C DC +++V+TKI+   S + C  + C  +I+ ++C  ++PK++ ++W K LCE 
Sbjct: 114 CTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTCRDLIPKDVFDRWEKILCES 173

Query: 67  LYSTEEKVVCPFKDCS-VGITLERG-ASIRDCECPLCHRLFCATCEVPWHDGVTCEEYKR 126
           L S+ +K  CPFKDCS + +  E G A++   EC  CHRLFC  C+V WH G+ C+E++R
Sbjct: 174 LISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHRLFCVQCKVTWHAGIGCDEFQR 233

Query: 127 L---YKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSAW 186
                K     ++ L+ ++A   +W +CP C F+V+K+ GC HI CRC ++FCY CGS W
Sbjct: 234 FGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKVEGCQHIKCRCGYQFCYGCGSVW 293

Query: 187 SSTHGGCQ 190
           SS+H  CQ
Sbjct: 294 SSSH-ACQ 300

[Close]
BLAST of CSPI07G19840 vs. TAIR10
Match: AT3G53690.1 (AT3G53690.1 RING/U-box superfamily protein)

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-48
Identity = 75/179 (41.90%), Postives = 111/179 (62.01%), Query Frame = 1 

Query: 2   FEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNK 61
           F I  C H +C DCV K+++ K+++   S+ C    C   ++   C ++LPKE+ ++W  
Sbjct: 128 FRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSGRLEPDQCRQILPKEVFDRWGD 187

Query: 62  ALCEVLYSTEEKVVCPFKDCSVGITLERG-ASIRDCECPLCHRLFCATCEVPWHDGVTCE 121
           ALCE +    +K  CP+KDCS  + LE     ++D ECP CHR+ C  C   WH  +TCE
Sbjct: 188 ALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPHCHRMVCVECGTQWHPEMTCE 247

Query: 122 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGS 180
           E+++L  NE GR++IL+  +A Q KW +CP C F++EK  GCL++ CRC   FCY CG+
Sbjct: 248 EFQKLAANERGRDDILLATMAKQKKWKRCPSCKFYIEKSQGCLYMKCRCGLAFCYNCGT 306

[Close]
BLAST of CSPI07G19840 vs. TAIR10
Match: AT5G37560.1 (AT5G37560.1 RING/U-box superfamily protein)

HSP 1 Score: 145.6 bits (366), Expect = 3.2e-35
Identity = 68/183 (37.16%), Postives = 101/183 (55.19%), Query Frame = 1 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           CLH  C  CV + V  K+ +G     CL   C S +   SC  +L  ++ E W + + E 
Sbjct: 199 CLHRHCFSCVKQQVKVKLRSGIVP-PCLEDGCKSELTLESCSMVLTPKLIEMWKRKMEED 258

Query: 67  LYSTEEKVVCPFKDCSVGIT---LERGASIRDCE-CPLCHRLFCATCEVPWHDGVTCEEY 126
           L    EK+ CP++ CS+ ++   L R A   +   C  C  LFC  C+VPWH  ++C +Y
Sbjct: 259 LIPDAEKIYCPYRSCSMLMSKTELSREAEQSNVRACIKCSELFCIDCKVPWHSDLSCADY 318

Query: 127 KRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSAWS 186
           KR++ +E   N++++K LA+   W +C +C   +E   GC HITCRC +EFCY CG  W+
Sbjct: 319 KRIH-SERLVNDMMLKVLANDQMWRQCSECKHMIELTEGCNHITCRCGYEFCYRCGHKWT 378

[Close]
BLAST of CSPI07G19840 vs. TAIR10
Match: AT2G21420.1 (AT2G21420.1 IBR domain containing protein)

HSP 1 Score: 144.1 bits (362), Expect = 9.3e-35
Identity = 73/194 (37.63%), Postives = 106/194 (54.64%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF    C H  C  CV +HV   +  G S  +CL   C S + F SC ++L   + E W 
Sbjct: 199 MFFTENCFHRQCFSCVNRHVQRMLLCGISP-TCLHFPCNSELTFESCSKVLTPNLIEFWK 258

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCE------CPLCHRLFCATCEVPWH 120
           + + E L    +K+ CP++ CS+   + + A  R+ +      C  C RLFC  C+VP H
Sbjct: 259 RKIEEDLVPAADKIYCPYRRCSM--LMSKTALSRETDQSNVRACIKCCRLFCIDCKVPSH 318

Query: 121 DGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFC 180
            G++C +YK+L  N +   ++ +K LA++ KW +C +C+  VE   GC HITCRC FEFC
Sbjct: 319 AGLSCVDYKKL--NPDPLYDVKLKSLANKKKWRQCVQCSNLVELFEGCNHITCRCGFEFC 378

Query: 181 YACGSAWSSTHGGC 189
           Y CG  W+    GC
Sbjct: 379 YVCGKEWNQR--GC 385

[Close]
BLAST of CSPI07G19840 vs. TAIR10
Match: AT5G60250.1 (AT5G60250.1 zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 142.9 bits (359), Expect = 2.1e-34
Identity = 69/199 (34.67%), Postives = 101/199 (50.75%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF ++ C H FC  CV +HV  K+ +G +   C    C S +   +C ++L  ++ + W 
Sbjct: 313 MFSVDKCRHRFCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQ 372

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITL----ERGASIRDC-------ECPLCHRLFCATC 120
           + L E      E+V CP+  CS  ++     E   S+           C  C  LFC  C
Sbjct: 373 QRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDC 432

Query: 121 EVPWHDGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRC 180
           +VPWH  ++C EYK+L+  E   +++ +K LA+   W +C KC   +E   GC HITCRC
Sbjct: 433 KVPWHGNLSCTEYKKLHP-EPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRC 492

Query: 181 RFEFCYACGSAWSSTHGGC 189
             EFCY CG  W+   G C
Sbjct: 493 GHEFCYNCGGGWNKIMGTC 509

[Close]
BLAST of CSPI07G19840 vs. NCBI nr
Match: gi|700189959|gb|KGN45192.1| (hypothetical protein Csa_7G430760 [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 1.0e-99
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN
Sbjct: 17  MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 76

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 120
           KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE
Sbjct: 77  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 136

Query: 121 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR 169
           EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKI GCLHITCR
Sbjct: 137 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKIYGCLHITCR 184

[Close]
BLAST of CSPI07G19840 vs. NCBI nr
Match: gi|659122572|ref|XP_008461214.1| (PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo])

HSP 1 Score: 276.6 bits (706), Expect = 3.4e-71
Identity = 119/143 (83.22%), Postives = 127/143 (88.81%), Query Frame = 1 

Query: 50  MLPKEIGEKWNKALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATC 109
           MLP E  EKWNKALCEV+Y  EEKVVCPFKDCSVG+ +ER A IRDCECPLCHRLFC TC
Sbjct: 1   MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC 60

Query: 110 EVPWHDGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRC 169
            VPWH+GVTCE+YKRLYK EE R+EILM+KLASQMKWMKCPKC  FVEK+SGCLHITCRC
Sbjct: 61  RVPWHEGVTCEDYKRLYKYEEIRDEILMEKLASQMKWMKCPKCQLFVEKVSGCLHITCRC 120

Query: 170 RFEFCYACGSAWSSTHGGCQPNY 193
           RFEFCYACGSAWSSTHGGC P Y
Sbjct: 121 RFEFCYACGSAWSSTHGGCLPYY 143

[Close]
BLAST of CSPI07G19840 vs. NCBI nr
Match: gi|502119893|ref|XP_004496771.1| (PREDICTED: probable E3 ubiquitin-protein ligase RNF144A [Cicer arietinum])

HSP 1 Score: 251.9 bits (642), Expect = 9.0e-64
Identity = 104/193 (53.89%), Postives = 135/193 (69.95%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF+   C HSFC +CV KHV+TKI+   + V C  LNC  +++   C  +LPKE+ ++W+
Sbjct: 158 MFKSQKCYHSFCTECVTKHVATKIQENITVVPCPGLNCKGLLELDVCRSLLPKEVIDRWD 217

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLER--GASIRDCECPLCHRLFCATCEVPWHDGVT 120
            ALCE L  +  K  CPFKDCS  +  E   G  IR+ ECP CHRLFCATC VPWH  V 
Sbjct: 218 DALCEALLLSVPKFYCPFKDCSAMLLDENEGGEEIRESECPFCHRLFCATCHVPWHPSVD 277

Query: 121 CEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACG 180
           C+E+++L  +E G  ++L+++LA+Q KW +CPKC F++EK  GCLHITCRCRFEFCYACG
Sbjct: 278 CDEFQKLNVDERGIQDLLVRELANQKKWKRCPKCKFYIEKFEGCLHITCRCRFEFCYACG 337

Query: 181 SAWSSTHGGCQPN 192
             W+S HGGCQ N
Sbjct: 338 EKWTSNHGGCQRN 350

[Close]
BLAST of CSPI07G19840 vs. NCBI nr
Match: gi|922406912|ref|XP_013469879.1| (zinc finger protein [Medicago truncatula])

HSP 1 Score: 251.1 bits (640), Expect = 1.5e-63
Identity = 101/187 (54.01%), Postives = 138/187 (73.80%), Query Frame = 1 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C HSFC +CV+K V+TKI++  ++VSC  LNC  +++  SC ++LPKE+ +KWN ALCE 
Sbjct: 121 CYHSFCSECVVKQVATKIQDNITNVSCPGLNCKGVLELESCRKLLPKELIDKWNDALCEA 180

Query: 67  LYSTEEKVVCPFKDCSVGITLERGA--SIRDCECPLCHRLFCATCEVPWHDGVTCEEYKR 126
            + T  K  CPFKDCS  +  E      +R+ EC  CHRLFCA C VPWH G+ CEEY++
Sbjct: 181 FFLTVPKFYCPFKDCSAMLLDEDEGREDVRESECASCHRLFCAKCHVPWHPGIGCEEYQK 240

Query: 127 LYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSAWSST 186
           L  +E GR ++L+++LA++ KW +CP+C F+VEK  GCLHITCRC+FEFCY+CG+ W+S+
Sbjct: 241 LNVDERGREDLLVRELANKKKWKRCPRCRFYVEKRDGCLHITCRCKFEFCYSCGAQWTSS 300

Query: 187 HGGCQPN 192
           HGGCQ N
Sbjct: 301 HGGCQRN 307

[Close]
BLAST of CSPI07G19840 vs. NCBI nr
Match: gi|694377993|ref|XP_009365261.1| (PREDICTED: probable E3 ubiquitin-protein ligase RNF217 isoform X3 [Pyrus x bretschneideri])

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-63
Identity = 99/191 (51.83%), Postives = 142/191 (74.35%), Query Frame = 1 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF    C+HSFC DC+ KHV++KI+     VSC +L+C ++++  +C  MLPKE+ E+WN
Sbjct: 126 MFRNEGCVHSFCSDCITKHVASKIQANIHVVSCPSLDCRAVLELDACMPMLPKEVIERWN 185

Query: 61  KALCEVLYSTEEKVVCPFKDCS--VGITLERGASIRDCECPLCHRLFCATCEVPWHDGVT 120
            ALCE +    +++ CPF DCS  + I +E   +IR+ ECP+CHRLFCA C++PWH GV 
Sbjct: 186 DALCEAMVLGAQRLYCPFSDCSTVLMIDIEGEEAIRESECPICHRLFCARCQIPWHPGVD 245

Query: 121 CEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACG 180
           CEE++RL ++E GR ++++++LA Q KW +CP+C ++VEK  GCLHITCRC++ FCY CG
Sbjct: 246 CEEFQRLNESERGRADLMVRELAKQKKWKRCPRCKYYVEKTQGCLHITCRCQYHFCYRCG 305

Query: 181 SAWSSTHGGCQ 190
           + W+STHGGCQ
Sbjct: 306 AEWTSTHGGCQ 316

The following BLAST results are available for this feature:
BLAST of CSPI07G19840 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RN217_XENLA1.3e-1728.49Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis GN=rnf217 PE=2 SV=... [more]
RN217_MOUSE2.8e-1727.66Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus GN=Rnf217 PE=3 SV=2[more]
ARI5_ARATH6.3e-1731.18Probable E3 ubiquitin-protein ligase ARI5 OS=Arabidopsis thaliana GN=ARI5 PE=2 S... [more]
RN217_HUMAN6.3e-1727.66Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens GN=RNF217 PE=2 SV=4[more]
ARI7_ARATH4.1e-1629.10Probable E3 ubiquitin-protein ligase ARI7 OS=Arabidopsis thaliana GN=ARI7 PE=2 S... [more]
BLAST of CSPI07G19840 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K9X9_CUCSA7.1e-10099.40Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430760 PE=4 SV=1[more]
A0A072VQ64_MEDTR1.1e-6354.01Uncharacterized protein OS=Medicago truncatula GN=MTR_1g105460 PE=4 SV=1[more]
A0A067LCT7_JATCU1.6e-5950.00Uncharacterized protein OS=Jatropha curcas GN=JCGZ_26932 PE=4 SV=1[more]
A0A059C8R9_EUCGR2.7e-5948.97Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E03591 PE=4 SV=1[more]
A0A068VLN7_COFCA3.5e-5948.40Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00003346001 PE=4 SV=1[more]
BLAST of CSPI07G19840 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G14250.16.0e-5042.02 RING/U-box superfamily protein[more]
AT3G53690.11.1e-4841.90 RING/U-box superfamily protein[more]
AT5G37560.13.2e-3537.16 RING/U-box superfamily protein[more]
AT2G21420.19.3e-3537.63 IBR domain containing protein[more]
AT5G60250.12.1e-3434.67 zinc finger (C3HC4-type RING finger) family protein[more]
BLAST of CSPI07G19840 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700189959|gb|KGN45192.1|1.0e-9999.40hypothetical protein Csa_7G430760 [Cucumis sativus][more]
gi|659122572|ref|XP_008461214.1|3.4e-7183.22PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo][more]
gi|502119893|ref|XP_004496771.1|9.0e-6453.89PREDICTED: probable E3 ubiquitin-protein ligase RNF144A [Cicer arietinum][more]
gi|922406912|ref|XP_013469879.1|1.5e-6354.01zinc finger protein [Medicago truncatula][more]
gi|694377993|ref|XP_009365261.1|2.0e-6351.83PREDICTED: probable E3 ubiquitin-protein ligase RNF217 isoform X3 [Pyrus x brets... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002867IBR_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0005515 protein binding
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G19840.1CSPI07G19840.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainPFAMPF01485IBRcoord: 136..188
score: 3.8E-9coord: 58..119
score: 1.
IPR002867IBR domainSMARTSM00647ibrneu5coord: 120..188
score: 0.0018coord: 57..119
score: 5.
NoneNo IPR availableunknownCoilCoilcoord: 119..139
scor
NoneNo IPR availablePANTHERPTHR11685:SF149SUBFAMILY NOT NAMEDcoord: 1..189
score: 2.7
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 59..124
score: 1.62E-8coord: 132..189
score: 4.24E-16coord: 3..66
score: 6.7