CsGy7G018130 (gene) Cucumber (Gy14) v2

Overview
NameCsGy7G018130
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationChr7 : 21257496 .. 21258366 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGAAATCAATACGTGTTTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCTACGAAGATCGAAAATGGATATTCCTCCGTTTCTTGTCTTGCTTTGAACTGTCCTTCCATTATAGATTTTCACTCTTGCTGGTAACTATAAGTAAATTTAGGGTTTAAGAAAGTTGAAGGGTTGGCGTAGAAATGACATATGGATCAATTACTTTACTTTGCAGGAGAATGCTACCGAAGGAAATAGGTGAGAAATGGAACAAAGCACTTTGCGAAGTGCTTTATTCGACAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCCGTGGGAATAACCCTTGAGAGGGGCGCAAGCATAAGGGACTGTGAGTGTCCGCTTTGCCATAGACTCTTTTGCGCGACATGTGAAGTCCCATGGCACGATGGTGTCACCTGCGAGGAATATAAGAGACTGTATAAAAATGAAGAAGGTAGAAATGAGATTTTGATGAAGAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCAATTTCTTCGTTGAGAAGATTTCTGGTTGTTTGCATATCACTTGCAGGTTCATTAATTTCTAATTCCCTCTTTCTTCTATTTTTATACACAACAGTCTAGACTATGTATCAGGACTTTCGATCAGTATTTGAGCCATATGAAGTGGAGGGATGAGTAATGTTAATAATTTATTTTGTATATATATATATATATAAATCATGATTACATATTAACATTCTAATTTGTATATATATATGGTTTATGAAATTGTTGTAGGTGTCGATTCGAGTTCTGCTATGCTTGTGGATCGGCTTGGTCTTCCACCCATGGTGGTTGCCAACTTAACTACTGA

mRNA sequence

ATGTTCGAAATCAATACGTGTTTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCTACGAAGATCGAAAATGGATATTCCTCCGTTTCTTGTCTTGCTTTGAACTGTCCTTCCATTATAGATTTTCACTCTTGCTGGAGAATGCTACCGAAGGAAATAGGTGAGAAATGGAACAAAGCACTTTGCGAAGTGCTTTATTCGACAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCCGTGGGAATAACCCTTGAGAGGGGCGCAAGCATAAGGGACTGTGAGTGTCCGCTTTGCCATAGACTCTTTTGCGCGACATGTGAAGTCCCATGGCACGATGGTGTCACCTGCGAGGAATATAAGAGACTGTATAAAAATGAAGAAGGTAGAAATGAGATTTTGATGAAGAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCAATTTCTTCGTTGAGAAGATTTCTGGTTGTTTGCATATCACTTGCAGGTGTCGATTCGAGTTCTGCTATGCTTGTGGATCGGCTTGGTCTTCCACCCATGGTGGTTGCCAACTTAACTACTGA

Coding sequence (CDS)

ATGTTCGAAATCAATACGTGTTTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCTACGAAGATCGAAAATGGATATTCCTCCGTTTCTTGTCTTGCTTTGAACTGTCCTTCCATTATAGATTTTCACTCTTGCTGGAGAATGCTACCGAAGGAAATAGGTGAGAAATGGAACAAAGCACTTTGCGAAGTGCTTTATTCGACAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCCGTGGGAATAACCCTTGAGAGGGGCGCAAGCATAAGGGACTGTGAGTGTCCGCTTTGCCATAGACTCTTTTGCGCGACATGTGAAGTCCCATGGCACGATGGTGTCACCTGCGAGGAATATAAGAGACTGTATAAAAATGAAGAAGGTAGAAATGAGATTTTGATGAAGAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCAATTTCTTCGTTGAGAAGATTTCTGGTTGTTTGCATATCACTTGCAGGTGTCGATTCGAGTTCTGCTATGCTTGTGGATCGGCTTGGTCTTCCACCCATGGTGGTTGCCAACTTAACTACTGA

Protein sequence

MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSAWSSTHGGCQLNY
Homology
[Close]
BLAST of CsGy7G018130 vs. NCBI nr
Match: KGN45192.1 (hypothetical protein Csa_7G430760 [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 2.0e-99
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN
Sbjct: 17  MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 76

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 120
           KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE
Sbjct: 77  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 136

Query: 121 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR 169
           EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKI GCLHITCR
Sbjct: 137 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKIYGCLHITCR 184

[Close]
BLAST of CsGy7G018130 vs. NCBI nr
Match: XP_008461214.1 (PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo])

HSP 1 Score: 271.6 bits (693), Expect = 2.1e-69
Identity = 117/139 (84.17%), Postives = 125/139 (89.93%), Query Frame = 0 

Query: 50  MLPKEIGEKWNKALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATC 109
           MLP E  EKWNKALCEV+Y  EEKVVCPFKDCSVG+ +ER A IRDCECPLCHRLFC TC
Sbjct: 1   MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC 60

Query: 110 EVPWHDGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRC 169
            VPWH+GVTCE+YKRLYK EE R+EILM+KLASQMKWMKCPKC  FVEK+SGCLHITCRC
Sbjct: 61  RVPWHEGVTCEDYKRLYKYEEIRDEILMEKLASQMKWMKCPKCQLFVEKVSGCLHITCRC 120

Query: 170 RFEFCYACGSAWSSTHGGC 189
           RFEFCYACGSAWSSTHGGC
Sbjct: 121 RFEFCYACGSAWSSTHGGC 139

[Close]
BLAST of CsGy7G018130 vs. NCBI nr
Match: XP_022146079.1 (probable E3 ubiquitin-protein ligase RNF217 [Momordica charantia])

HSP 1 Score: 269.6 bits (688), Expect = 8.0e-69
Identity = 118/195 (60.51%), Postives = 147/195 (75.38%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF I  CLHSFCR CVIKHV+ KI  G S VSC +L+C   ++F +CW MLPK + EKW+
Sbjct: 49  MFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWD 108

Query: 61  KALCEVLYSTEEKVVCPFKDCSVG-ITLERG--ASIRDCECPLCHRLFCATCEVPWHDGV 120
           +ALCE L+   EKV+CPFKDCS   + LE+G  A I + ECP+CHRLFCA C VPWH  V
Sbjct: 109 RALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARV 168

Query: 121 TCEEYKRLYKNEE-GR--NEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFC 180
           TCEEY+R  K+++ GR  ++ L+K +A +M+WM+CP+C FFVEK  GCLHITCRC+FEFC
Sbjct: 169 TCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFC 228

Query: 181 YACGSAWSSTHGGCQ 190
           YACGS WS  HGGC+
Sbjct: 229 YACGSQWSHNHGGCR 243

[Close]
BLAST of CsGy7G018130 vs. NCBI nr
Match: GAU14896.1 (hypothetical protein TSUD_46890 [Trifolium subterraneum])

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-65
Identity = 108/194 (55.67%), Postives = 138/194 (71.13%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF    C HSFC +CVIK V TKI    ++VSC  LNC  +I+  SC  +LPKE+ +KWN
Sbjct: 99  MFNNQNCSHSFCSECVIKQVETKINEKIANVSCPGLNCKGVIELESCISLLPKELIDKWN 158

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLER---GASIRDCECPLCHRLFCATCEVPWHDGV 120
            ALCE L+ +  K  CPFKDCS  +  E    G  IR+ ECP CHRLFCA+C VPWH G+
Sbjct: 159 DALCEALFCSVPKFYCPFKDCSAMLLDENEGGGEDIRESECPFCHRLFCASCHVPWHPGI 218

Query: 121 TCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYAC 180
            CE+Y++L  +E G +++++++LA++ KW +CP C F+VEK  GCLH+TCRCRFEFCYAC
Sbjct: 219 GCEDYQKLNVDERGSDDLVVRELANEKKWRRCPTCKFYVEKNGGCLHVTCRCRFEFCYAC 278

Query: 181 GSAWSSTHGGCQLN 192
           G  WSSTHGGCQ N
Sbjct: 279 GEQWSSTHGGCQTN 292

[Close]
BLAST of CsGy7G018130 vs. NCBI nr
Match: PNX98112.1 (putative E3 ubiquitin-protein ligase RNF144A-like protein [Trifolium pratense])

HSP 1 Score: 256.9 bits (655), Expect = 5.4e-65
Identity = 108/193 (55.96%), Postives = 138/193 (71.50%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF   +C HSFC +CVIK V TKI    ++VSC  LNC  +++  SC  +LPKE+ +KWN
Sbjct: 102 MFNNQSCSHSFCSECVIKQVETKINEKIANVSCPGLNCKGVLELESCISLLPKELIDKWN 161

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGA--SIRDCECPLCHRLFCATCEVPWHDGVT 120
            ALCE L+ +  K  CPFKDCS  +  E      IR+ ECP CHRLFCA+C VPWH G+ 
Sbjct: 162 DALCEALFCSVPKFYCPFKDCSAMLLDENEGVEDIRESECPFCHRLFCASCHVPWHPGIG 221

Query: 121 CEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACG 180
           CEEY++L  +E G +++++++LA+Q +W +CP C F+VEK  GCLHITCRCRFEFCYACG
Sbjct: 222 CEEYQKLNVDERGSDDLVVRELANQKQWRRCPTCKFYVEKNGGCLHITCRCRFEFCYACG 281

Query: 181 SAWSSTHGGCQLN 192
             WSSTHGGCQ N
Sbjct: 282 EQWSSTHGGCQKN 294

[Close]
BLAST of CsGy7G018130 vs. TAIR10
Match: AT3G14250.1 (RING/U-box superfamily protein)

HSP 1 Score: 194.9 bits (494), Expect = 4.6e-50
Identity = 79/189 (41.80%), Postives = 123/189 (65.08%), Query Frame = 0 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C H++C DC +++V+TKI+   S + C  + C  +I+ ++C  ++PK++ ++W K LCE 
Sbjct: 114 CTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTCRDLIPKDVFDRWEKILCES 173

Query: 67  LYSTEEKVVCPFKDCS-VGITLERG-ASIRDCECPLCHRLFCATCEVPWHDGVTCEEYKR 126
           L S+ +K  CPFKDCS + +  E G A++   EC  CHRLFC  C+V WH G+ C+E++R
Sbjct: 174 LISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHRLFCVQCKVTWHAGIGCDEFQR 233

Query: 127 L---YKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSAW 186
                K     ++ L+ ++A   +W +CP C F+V+K+ GC HI CRC ++FCY CGS W
Sbjct: 234 FGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKVEGCQHIKCRCGYQFCYGCGSVW 293

Query: 187 SSTHGGCQL 191
           SS+H  CQ+
Sbjct: 294 SSSH-ACQI 301

[Close]
BLAST of CsGy7G018130 vs. TAIR10
Match: AT3G53690.1 (RING/U-box superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 1.5e-48
Identity = 75/179 (41.90%), Postives = 111/179 (62.01%), Query Frame = 0 

Query: 2   FEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNK 61
           F I  C H +C DCV K+++ K+++   S+ C    C   ++   C ++LPKE+ ++W  
Sbjct: 128 FRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSGRLEPDQCRQILPKEVFDRWGD 187

Query: 62  ALCEVLYSTEEKVVCPFKDCSVGITLERG-ASIRDCECPLCHRLFCATCEVPWHDGVTCE 121
           ALCE +    +K  CP+KDCS  + LE     ++D ECP CHR+ C  C   WH  +TCE
Sbjct: 188 ALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPHCHRMVCVECGTQWHPEMTCE 247

Query: 122 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGS 180
           E+++L  NE GR++IL+  +A Q KW +CP C F++EK  GCL++ CRC   FCY CG+
Sbjct: 248 EFQKLAANERGRDDILLATMAKQKKWKRCPSCKFYIEKSQGCLYMKCRCGLAFCYNCGT 306

[Close]
BLAST of CsGy7G018130 vs. TAIR10
Match: AT5G37560.1 (RING/U-box superfamily protein)

HSP 1 Score: 146.7 bits (369), Expect = 1.4e-35
Identity = 71/189 (37.57%), Postives = 104/189 (55.03%), Query Frame = 0 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           CLH  C  CV + V  K+ +G     CL   C S +   SC  +L  ++ E W + + E 
Sbjct: 199 CLHRHCFSCVKQQVKVKLRSGIVP-PCLEDGCKSELTLESCSMVLTPKLIEMWKRKMEED 258

Query: 67  LYSTEEKVVCPFKDCSVGIT---LERGASIRDCE-CPLCHRLFCATCEVPWHDGVTCEEY 126
           L    EK+ CP++ CS+ ++   L R A   +   C  C  LFC  C+VPWH  ++C +Y
Sbjct: 259 LIPDAEKIYCPYRSCSMLMSKTELSREAEQSNVRACIKCSELFCIDCKVPWHSDLSCADY 318

Query: 127 KRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACGSAWS 186
           KR++ +E   N++++K LA+   W +C +C   +E   GC HITCRC +EFCY CG  W+
Sbjct: 319 KRIH-SERLVNDMMLKVLANDQMWRQCSECKHMIELTEGCNHITCRCGYEFCYRCGHKWT 378

Query: 187 STHG-GCQL 191
             H   CQL
Sbjct: 379 KYHHYSCQL 385

[Close]
BLAST of CsGy7G018130 vs. TAIR10
Match: AT2G21420.1 (IBR domain containing protein)

HSP 1 Score: 143.3 bits (360), Expect = 1.6e-34
Identity = 73/194 (37.63%), Postives = 106/194 (54.64%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF    C H  C  CV +HV   +  G S  +CL   C S + F SC ++L   + E W 
Sbjct: 199 MFFTENCFHRQCFSCVNRHVQRMLLCGISP-TCLHFPCNSELTFESCSKVLTPNLIEFWK 258

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCE------CPLCHRLFCATCEVPWH 120
           + + E L    +K+ CP++ CS  + + + A  R+ +      C  C RLFC  C+VP H
Sbjct: 259 RKIEEDLVPAADKIYCPYRRCS--MLMSKTALSRETDQSNVRACIKCCRLFCIDCKVPSH 318

Query: 121 DGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFC 180
            G++C +YK+L  N +   ++ +K LA++ KW +C +C+  VE   GC HITCRC FEFC
Sbjct: 319 AGLSCVDYKKL--NPDPLYDVKLKSLANKKKWRQCVQCSNLVELFEGCNHITCRCGFEFC 378

Query: 181 YACGSAWSSTHGGC 189
           Y CG  W+    GC
Sbjct: 379 YVCGKEWNQR--GC 385

[Close]
BLAST of CsGy7G018130 vs. TAIR10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 142.5 bits (358), Expect = 2.7e-34
Identity = 69/201 (34.33%), Postives = 102/201 (50.75%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF ++ C H FC  CV +HV  K+ +G +   C    C S +   +C ++L  ++ + W 
Sbjct: 313 MFSVDKCRHRFCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQ 372

Query: 61  KALCEVLYSTEEKVVCPFKDCSV-------------GITLERGASIRDCECPLCHRLFCA 120
           + L E      E+V CP+  CS               ++L   + +R   C  C  LFC 
Sbjct: 373 QRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVR--RCVECRGLFCV 432

Query: 121 TCEVPWHDGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITC 180
            C+VPWH  ++C EYK+L+  E   +++ +K LA+   W +C KC   +E   GC HITC
Sbjct: 433 DCKVPWHGNLSCTEYKKLHP-EPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITC 492

Query: 181 RCRFEFCYACGSAWSSTHGGC 189
           RC  EFCY CG  W+   G C
Sbjct: 493 RCGHEFCYNCGGGWNKIMGTC 509

[Close]
BLAST of CsGy7G018130 vs. Swiss-Prot
Match: sp|Q4KLT0|RN217_XENLA (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.7e-17
Identity = 53/186 (28.49%), Postives = 83/186 (44.62%), Query Frame = 0 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWNKALCEV 66
           C    C +C+ +++S++++ G + + C    C   +D  +    LP +   K+ K   E+
Sbjct: 18  CKKPVCDECLKRYLSSQVQLGQAEIQCPITECNKHLDESTILYSLPHDDIIKY-KYFLEL 77

Query: 67  LYSTEEKVVCPFKDCSVGITLERGASIRD---------CECPLCHRLFCATCEVPWHDGV 126
                    CP   C    T +R   I +          +CP C  ++C  C  PWH+GV
Sbjct: 78  SRMDSSTKPCP--QCKHFTTFKRKTHIPNPTKSENKLKIQCPSCQFIWCFRCHAPWHEGV 137

Query: 127 TCEEYKRLYK-NEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITC-RCRFEFCY 182
            C EYK+  K      NEI       Q    KCP+C   +++  GC H+TC +C   FCY
Sbjct: 138 NCREYKKGDKLLRHWANEI----EHGQRNAQKCPRCKVHIQRTEGCDHMTCSQCNTNFCY 196

[Close]
BLAST of CsGy7G018130 vs. Swiss-Prot
Match: sp|D3YYI7|RN217_MOUSE (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 2.9e-17
Identity = 53/187 (28.34%), Postives = 87/187 (46.52%), Query Frame = 0 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKE--IGEKWNKALC 66
           C  + C +C+  ++S++++ G   + C    C   ++  +    L  E  I  K+   L 
Sbjct: 251 CKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLEETTVVYNLTHEDSIKYKYFLELG 310

Query: 67  EVLYSTEEKVVC----PFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 126
            +  ST+    C     FK      T  R  S    +CP C  ++C  C  PWH+GV C+
Sbjct: 311 RIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYKIQCPTCQLIWCFKCHSPWHEGVNCK 370

Query: 127 EYKRLYKNEEGRNEILMKKLASQMKW-----MKCPKCNFFVEKISGCLHITC-RCRFEFC 182
           EYK        + + L++  AS+++       KCPKC   +++  GC H+TC +C   FC
Sbjct: 371 EYK--------KGDKLLRHWASEIEHGQRNAQKCPKCKIHIQRTEGCDHMTCSQCNTNFC 429

[Close]
BLAST of CsGy7G018130 vs. Swiss-Prot
Match: sp|Q8TC41|RN217_HUMAN (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 89.0 bits (219), Expect = 6.4e-17
Identity = 53/187 (28.34%), Postives = 86/187 (45.99%), Query Frame = 0 

Query: 7   CLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKE--IGEKWNKALC 66
           C  + C +C+  ++S +++ G   + C    C   ++  +    L  E  I  K+   L 
Sbjct: 278 CKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLEETTVVYNLTHEDSIKYKYFLELG 337

Query: 67  EVLYSTEEKVVC----PFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 126
            +  ST+    C     FK      T  R  S    +CP C  ++C  C  PWH+GV C+
Sbjct: 338 RIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYKIQCPTCQFVWCFKCHSPWHEGVNCK 397

Query: 127 EYKRLYKNEEGRNEILMKKLASQMKW-----MKCPKCNFFVEKISGCLHITC-RCRFEFC 182
           EYK        + + L++  AS+++       KCPKC   +++  GC H+TC +C   FC
Sbjct: 398 EYK--------KGDKLLRHWASEIEHGQRNAQKCPKCKIHIQRTEGCDHMTCSQCNTNFC 456

[Close]
BLAST of CsGy7G018130 vs. Swiss-Prot
Match: sp|Q8L829|ARI5_ARATH (Probable E3 ubiquitin-protein ligase ARI5 OS=Arabidopsis thaliana OX=3702 GN=ARI5 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.1e-16
Identity = 58/186 (31.18%), Postives = 82/186 (44.09%), Query Frame = 0 

Query: 6   TCLHSFCRDCVIKHVSTKIENGYS--SVSCLALNCPSIIDFHSCWRMLPKEIGEKWNK-A 65
           +C H FC  C   ++ST I +G     + C   +CP+ I      ++  KE  EK+ +  
Sbjct: 148 SCGHPFCATCWTGYISTTINDGPGCLMLKCPDPSCPAAIGRDMIDKLASKEDKEKYYRYF 207

Query: 66  LCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCEEY 125
           L   +    E   CP   C   I    G    D  C LC   FC  C    H  V C+  
Sbjct: 208 LRSYVEVNREMKWCPAPGCEHAIDFAGGTESYDVSC-LCSHSFCWNCTEEAHRPVDCDTV 267

Query: 126 KRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR--CRFEFCYACGSA 185
            +        +E +   LA+      CPKC   +EK  GC+H+TC   C+FEFC+ C +A
Sbjct: 268 GKWILKNSAESENMNWILANS---KPCPKCKRPIEKNHGCMHMTCTPPCKFEFCWLCLNA 327

Query: 186 WSSTHG 187
           W+  HG
Sbjct: 328 WTE-HG 328

[Close]
BLAST of CsGy7G018130 vs. Swiss-Prot
Match: sp|A2VEA3|ARI1_BOVIN (E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.1e-16
Identity = 56/192 (29.17%), Postives = 89/192 (46.35%), Query Frame = 0 

Query: 7   CLHSFCRDCVIKHVSTKI--ENGYSSVSCLALNCPSIIDFHSCWRML-PKEIGEKWNKAL 66
           C H FC  C  ++++TKI  E    ++SC A  C  ++D ++  R++   ++  K+   +
Sbjct: 201 CGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLI 260

Query: 67  CEVLYSTEEKVV-CPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCEEY 126
                     +  CP  DC   + ++     +   C  C R FC  C   WHD V C+  
Sbjct: 261 TNSFVECNRLLKWCPAPDCHHVVKVQY-PDAKPVRCK-CGRQFCFNCGENWHDPVKCKWL 320

Query: 127 KRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR---CRFEFCYACGS 186
           K+  K  +  +E     +A+  K  +CPKC+  +EK  GC H+ CR   C+ EFC+ C  
Sbjct: 321 KKWIKKCDDDSE-TSNWIAANTK--ECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLG 380

Query: 187 AWSSTHGGCQLN 192
            W   HG    N
Sbjct: 381 PW-EPHGSAWYN 386

[Close]
BLAST of CsGy7G018130 vs. TrEMBL
Match: tr|A0A0A0K9X9|A0A0A0K9X9_CUCSA (RBR-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G430760 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.3e-99
Identity = 167/168 (99.40%), Postives = 167/168 (99.40%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN
Sbjct: 17  MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 76

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 120
           KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE
Sbjct: 77  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 136

Query: 121 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCR 169
           EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKI GCLHITCR
Sbjct: 137 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKIYGCLHITCR 184

[Close]
BLAST of CsGy7G018130 vs. TrEMBL
Match: tr|A0A1S3CDQ5|A0A1S3CDQ5_CUCME (RBR-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499864 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.4e-69
Identity = 117/139 (84.17%), Postives = 125/139 (89.93%), Query Frame = 0 

Query: 50  MLPKEIGEKWNKALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATC 109
           MLP E  EKWNKALCEV+Y  EEKVVCPFKDCSVG+ +ER A IRDCECPLCHRLFC TC
Sbjct: 1   MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC 60

Query: 110 EVPWHDGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRC 169
            VPWH+GVTCE+YKRLYK EE R+EILM+KLASQMKWMKCPKC  FVEK+SGCLHITCRC
Sbjct: 61  RVPWHEGVTCEDYKRLYKYEEIRDEILMEKLASQMKWMKCPKCQLFVEKVSGCLHITCRC 120

Query: 170 RFEFCYACGSAWSSTHGGC 189
           RFEFCYACGSAWSSTHGGC
Sbjct: 121 RFEFCYACGSAWSSTHGGC 139

[Close]
BLAST of CsGy7G018130 vs. TrEMBL
Match: tr|A0A2K3N511|A0A2K3N511_TRIPR (RBR-type E3 ubiquitin transferase OS=Trifolium pratense OX=57577 GN=L195_g021354 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.6e-65
Identity = 108/193 (55.96%), Postives = 138/193 (71.50%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF   +C HSFC +CVIK V TKI    ++VSC  LNC  +++  SC  +LPKE+ +KWN
Sbjct: 102 MFNNQSCSHSFCSECVIKQVETKINEKIANVSCPGLNCKGVLELESCISLLPKELIDKWN 161

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGA--SIRDCECPLCHRLFCATCEVPWHDGVT 120
            ALCE L+ +  K  CPFKDCS  +  E      IR+ ECP CHRLFCA+C VPWH G+ 
Sbjct: 162 DALCEALFCSVPKFYCPFKDCSAMLLDENEGVEDIRESECPFCHRLFCASCHVPWHPGIG 221

Query: 121 CEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACG 180
           CEEY++L  +E G +++++++LA+Q +W +CP C F+VEK  GCLHITCRCRFEFCYACG
Sbjct: 222 CEEYQKLNVDERGSDDLVVRELANQKQWRRCPTCKFYVEKNGGCLHITCRCRFEFCYACG 281

Query: 181 SAWSSTHGGCQLN 192
             WSSTHGGCQ N
Sbjct: 282 EQWSSTHGGCQKN 294

[Close]
BLAST of CsGy7G018130 vs. TrEMBL
Match: tr|A0A2K3N045|A0A2K3N045_TRIPR (RBR-type E3 ubiquitin transferase OS=Trifolium pratense OX=57577 GN=L195_g019626 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.0e-63
Identity = 106/193 (54.92%), Postives = 136/193 (70.47%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF    C HSFC +CV+K VSTKI++  ++VSC  LNC  +++  SC  +LPK + ++W+
Sbjct: 139 MFRNQRCYHSFCSECVVKQVSTKIQDNITNVSCPGLNCKGLLELESCRSLLPKALIDRWD 198

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERG--ASIRDCECPLCHRLFCATCEVPWHDGVT 120
            AL E L  T  K  CPFKDCS  +  E      IR+ ECP CHRLFCA C VPWH GV 
Sbjct: 199 DALSEALLLTVPKFYCPFKDCSAMLLGENEGVGDIRESECPFCHRLFCARCYVPWHPGVG 258

Query: 121 CEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACG 180
           CEEY+++  +E GR ++L+K+LA Q KW +CP+C F+VEK  GCLHITCRC+FEFCYACG
Sbjct: 259 CEEYQKMNPDERGREDLLVKELAKQKKWKRCPRCKFYVEKRDGCLHITCRCKFEFCYACG 318

Query: 181 SAWSSTHGGCQLN 192
             W++THGGCQ N
Sbjct: 319 ERWTTTHGGCQRN 331

[Close]
BLAST of CsGy7G018130 vs. TrEMBL
Match: tr|A0A1S2XZ66|A0A1S2XZ66_CICAR (RBR-type E3 ubiquitin transferase OS=Cicer arietinum OX=3827 GN=LOC101504799 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.0e-63
Identity = 104/193 (53.89%), Postives = 135/193 (69.95%), Query Frame = 0 

Query: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60
           MF+   C HSFC +CV KHV+TKI+   + V C  LNC  +++   C  +LPKE+ ++W+
Sbjct: 158 MFKSQKCYHSFCTECVTKHVATKIQENITVVPCPGLNCKGLLELDVCRSLLPKEVIDRWD 217

Query: 61  KALCEVLYSTEEKVVCPFKDCSVGITLER--GASIRDCECPLCHRLFCATCEVPWHDGVT 120
            ALCE L  +  K  CPFKDCS  +  E   G  IR+ ECP CHRLFCATC VPWH  V 
Sbjct: 218 DALCEALLLSVPKFYCPFKDCSAMLLDENEGGEEIRESECPFCHRLFCATCHVPWHPSVD 277

Query: 121 CEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKISGCLHITCRCRFEFCYACG 180
           C+E+++L  +E G  ++L+++LA+Q KW +CPKC F++EK  GCLHITCRCRFEFCYACG
Sbjct: 278 CDEFQKLNVDERGIQDLLVRELANQKKWKRCPKCKFYIEKFEGCLHITCRCRFEFCYACG 337

Query: 181 SAWSSTHGGCQLN 192
             W+S HGGCQ N
Sbjct: 338 EKWTSNHGGCQRN 350

The following BLAST results are available for this feature:
BLAST of CsGy7G018130 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN45192.12.0e-9999.40hypothetical protein Csa_7G430760 [Cucumis sativus][more]
XP_008461214.12.1e-6984.17PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo][more]
XP_022146079.18.0e-6960.51probable E3 ubiquitin-protein ligase RNF217 [Momordica charantia][more]
GAU14896.11.4e-6555.67hypothetical protein TSUD_46890 [Trifolium subterraneum][more]
PNX98112.15.4e-6555.96putative E3 ubiquitin-protein ligase RNF144A-like protein [Trifolium pratense][more]
BLAST of CsGy7G018130 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G14250.14.6e-5041.80RING/U-box superfamily protein[more]
AT3G53690.11.5e-4841.90RING/U-box superfamily protein[more]
AT5G37560.11.4e-3537.57RING/U-box superfamily protein[more]
AT2G21420.11.6e-3437.63IBR domain containing protein[more]
AT5G60250.12.7e-3434.33zinc finger (C3HC4-type RING finger) family protein[more]
BLAST of CsGy7G018130 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q4KLT0|RN217_XENLA1.7e-1728.49Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 ... [more]
sp|D3YYI7|RN217_MOUSE2.9e-1728.34Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P... [more]
sp|Q8TC41|RN217_HUMAN6.4e-1728.34Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE... [more]
sp|Q8L829|ARI5_ARATH1.1e-1631.18Probable E3 ubiquitin-protein ligase ARI5 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
sp|A2VEA3|ARI1_BOVIN4.1e-1629.17E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1[more]
BLAST of CsGy7G018130 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0K9X9|A0A0A0K9X9_CUCSA1.3e-9999.40RBR-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G430760 PE=... [more]
tr|A0A1S3CDQ5|A0A1S3CDQ5_CUCME1.4e-6984.17RBR-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499864 PE=3 S... [more]
tr|A0A2K3N511|A0A2K3N511_TRIPR3.6e-6555.96RBR-type E3 ubiquitin transferase OS=Trifolium pratense OX=57577 GN=L195_g021354... [more]
tr|A0A2K3N045|A0A2K3N045_TRIPR2.0e-6354.92RBR-type E3 ubiquitin transferase OS=Trifolium pratense OX=57577 GN=L195_g019626... [more]
tr|A0A1S2XZ66|A0A1S2XZ66_CICAR2.0e-6353.89RBR-type E3 ubiquitin transferase OS=Cicer arietinum OX=3827 GN=LOC101504799 PE=... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
Vocabulary: INTERPRO
TermDefinition
IPR031127E3_UB_ligase_RBR
IPR013083Znf_RING/FYVE/PHD
IPR002867IBR_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G018130.1CsGy7G018130.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 119..139
NoneNo IPR availableGENE3DG3DSA:1.20.120.1750coord: 115..192
e-value: 1.2E-12
score: 50.1
NoneNo IPR availablePANTHERPTHR11685:SF205RING/U-BOX SUPERFAMILY PROTEINcoord: 1..190
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 3..66
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 59..124
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 132..189
IPR002867IBR domainSMARTSM00647ibrneu5coord: 57..119
e-value: 5.2E-9
score: 45.9
coord: 120..188
e-value: 0.0018
score: 27.5
IPR002867IBR domainPFAMPF01485IBRcoord: 136..188
e-value: 3.9E-9
score: 36.5
coord: 58..119
e-value: 1.8E-8
score: 34.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 1..71
e-value: 2.6E-12
score: 48.6
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 1..190

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CsGy7G018130Cla015300Watermelon (97103) v1cgybwmB528
CsGy7G018130Csa7G430760Cucumber (Chinese Long) v2cgybcuB323
CsGy7G018130ClCG09G003550Watermelon (Charleston Gray)cgybwcgB484
CsGy7G018130CSPI07G19840Wild cucumber (PI 183967)cgybcpiB331
CsGy7G018130Lsi02G026390Bottle gourd (USVL1VR-Ls)cgyblsiB472
CsGy7G018130MELO3C029245.2Melon (DHL92) v3.6.1cgybmedB455
CsGy7G018130CsaV3_7G031650Cucumber (Chinese Long) v3cgybcucB347
CsGy7G018130Cla97C09G165800Watermelon (97103) v2cgybwmbB541
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CsGy7G018130Wild cucumber (PI 183967)cgybcpiB313
CsGy7G018130Silver-seed gourdcarcgybB0366
CsGy7G018130Silver-seed gourdcarcgybB0461
CsGy7G018130Wax gourdcgybwgoB673
CsGy7G018130Watermelon (97103) v2cgybwmbB490
CsGy7G018130Cucumber (Gy14) v2cgybcgybB113
CsGy7G018130Cucurbita maxima (Rimu)cgybcmaB911
CsGy7G018130Cucurbita maxima (Rimu)cgybcmaB925
CsGy7G018130Cucurbita maxima (Rimu)cgybcmaB938
CsGy7G018130Cucurbita maxima (Rimu)cgybcmaB973
CsGy7G018130Cucurbita moschata (Rifu)cgybcmoB893
CsGy7G018130Cucurbita moschata (Rifu)cgybcmoB909
CsGy7G018130Cucurbita moschata (Rifu)cgybcmoB921
CsGy7G018130Cucurbita moschata (Rifu)cgybcmoB955
CsGy7G018130Cucurbita pepo (Zucchini)cgybcpeB906
CsGy7G018130Cucurbita pepo (Zucchini)cgybcpeB931
CsGy7G018130Cucurbita pepo (Zucchini)cgybcpeB946
CsGy7G018130Cucumber (Chinese Long) v2cgybcuB305
CsGy7G018130Bottle gourd (USVL1VR-Ls)cgyblsiB483
CsGy7G018130Melon (DHL92) v3.5.1cgybmeB457
CsGy7G018130Watermelon (Charleston Gray)cgybwcgB492
CsGy7G018130Watermelon (97103) v1cgybwmB537