CSPI07G03400 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI07G03400
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Glucosidase 2 subunit beta
Location	Chr7 : 2688720 .. 2691796 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCATTATTTAAAAAGATAAAACAATTTAATTGTTCATATTATATTCCAACGTTGGTAAAAAGGTAATAACCATTTTGATTAGCCCAAATGGGAACGTCCGAGCGGGGAAGACCGGCACTCGGCTAGTCAAATCTCCGGACTGACTTGCTATTGCGTTCACTCCTTCATCAATTTCACCTCCTCCAATTGCATTGAAGCTTAATCAGACATGGAACCCCGCTCTCCAAAGTCTACCTCCTTCGCTTCACTGCTTTCCATATGTTTATTCTTCACGTCTGTTCGTTCTGCTCCATCATTCATCGGAGTTCACCCCCTCGGTACCTTTCTCACTCTCTTTCTCTCTCTCTCTCTCTATCTCTCTATCTCACTCTGTTCTTAATGATCCCCCTTGATCTGATTTTTCGAAGCTCAATTATTGAATTTTTATCCCCATTTCTCACTCTCCAGCTAATTCCCCTTTTTGGGGTATACCCGTGTTGTGAATTGATGCTAAATTCGTCTTTTTTTGGTGAATTTTCCTGAATTGAAACGTGTGTTCGATTGCAGATGAGAAGTATTATTCCTCCGAGGTCATAAAATGCAAGGATGGTTCTAGGTCTTTTACTATAGATCGTCTCAATGACAATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTGTGTTCTTACTTCTTACTAAAATCTAGTCATTAGAGTTACTGCTATTATAAGATGAACCTCAAGTTGAAATAGCTGTGAAAGTATGTATTGAACTAAACGCAACGATTACTAGTCATTGTATTATGTTTAACTATTGAAGAAGACTTGCCATGTTTAACCTAGAAAAGCTTGACTTGGTTTTTCTTTCTGAATTTTCATCAACTTGATACTGTATCTATTTTGTCCTGTATCATTAGAATGTAGGAATAGGATAGGGAAAATCGTTAGAAATACAAAATAGCACTTTTTGGCGAATTGTTCTAATGACACATCTTGGTGTGTCATGATCAAAACCGAGACTAATACAAAAACCAAAACTCAACTTTTTAAATGGCATATCCAAAGGAATGATTTTTTTGGGCTTTTTCTCGGTGGAAACTCGGCCAGAAGATCAAGACGAACTGCTTTTTTTCTGCCTAAGCTCCCAAAATCTTATTCCCTCCTTATCATAGACACTTTCCGATAAGATCACATACATAATATTTGGAAATGTTTGCGAAAGATTTGGAGAATGCACGCATAAATTCAAAATAATAGTCAGTCCCAGTATTATCAAGGAGTTCCACCCAAAAAAATTGATGGTCTGAATGTGCCACTAAAAGAAGCTAAATTTTTGTTTGGGTCTCCGTTTCAATTCTGTATGAAAGGTACCATGGTGAAATATGAAAACAATTGGCCAACAAGGGTGCTATTTGTGAATGGTGGAAAATTCAAAATTGAGTGCTATTTCTAAGTAATAAATTCAAATTCCAGCATGGGCAATTGGTCAAGTGAAAAAAAGGAAAACAAACCTTCCCAAATAGCCCAAGAACAGCCCACACTCAGGCACACACTCACTAACCCAGTGCAAGGATCAGACAAGTTTTTTTTCTGTGCTCTGCTGCTGCTGGCTCATGGATTTGACCTCTGATCTTTGTATTTATGTATGTAGGTACATCTGCCTGTGCAAGAGGGAAATTCTATTGTCGGAATATGGGAAGTACACCACGGTTTATTTTTTCTTCCCGTGTCAACGATCATATATGTGGTAACTATCTATCAGATCTTTTAATTTCCTTATTTTTTTATGCATACGACTCAGAAAAGCATGTCGATTGAAACACCATAAATTTATTGATTTGGATTTTAAAACAATCTTTCTGTATGGTGTCTGTAATGCAATGAGACAGTCTTATGGCTCATTCACTACTTTTCCTGATTATTTGTTCTTCCTGCTTTATGTTAAGACAATTTAAGGTCTACATTCAGGTTAAAATCTGCAATTAGTGGTTTAAACTTGACAATCAGCATTCCATTTCTCTAAAACTTTTCCACATAAACCATTTTGTGACATTTCCATAGTCTATCTGCCTTTTTTTTCTACTTATGTTGAATCCACAATTAATTAGCGAAGCTTGGGGTTTTAGTTTCATTTTTAAAATTAAATTCTAGCTCCCTGTTCAATATTTATTTGTAGATTGCTGTGATGGAAGTGATGAATATGAGGGTAATATCTTTTGCCCGAACACATGTGTCATGGGTGGGAATATGTACAAGAGCAAAAATGATATTTCAACCACCCGAGATGTTGATATAGTCATCAGAAAAGTTAAGGAAGAAATCACCAAGGAGGACTTGTTCCAAAAGCTAACTGGTAGGATTTTTTTTTCAATCATTACAAAACAGATACACAATACTCAACTGATCATTGTTGTAACTCTTAACTTGCAATTTTCAGGTTTGAAGCTAGTAATAGTCCTACAGGTAGCTCTCACAAGTTTTGCAATCCTCATTTGGGCAAACCGTTGTCGTGTGAAATCTAAGAGAAGACGACACCGTTGAAGCGAGCCGCAATCGTCATTCCTTAATCTTCACTATAATCCCAAGTACATCTTCCAGTTCCATATATAACTTCTCCAGTCTATCCTCTTAGTCTAAATTGTTTATCAATCAATGTTCATTCATTCTCCAGGCTTCTCTTATCAGCACGATTACGTGATGTCCTTCTTTCAGATGCTCACAAAGCTCAACACGCTGGATAAAGAACTACTGATCCATTAGTCTTAACAGACCATGATAGATTAGCTGCCTAAACCCAACCTTGTTATTTATATATAAAGATCACAACAACACAATGGATTGCCTACCCAATCTATATCAATTTTGTAACATTTGGGCTAGATTCACTCAATGTACCATATCAAAAAGAACAAATTTTGTTACAATGTTTGTCTTGCACGACCTCAATAACTTTTGTCGAAGTTTGCAGCCTTTGATTATGCAATGAACCAAATGAACGTTGTGTAAAAGTAAAATCTGGTGTACATGAAAGAGGGCAAGTTTTCCAAAAAGGGAAACTGGGGTCTCATTTTCAACCTCAAATTCATCTATTCCCA

mRNA sequence

ATGGAACCCCGCTCTCCAAAGTCTACCTCCTTCGCTTCACTGCTTTCCATATGTTTATTCTTCACGTCTGTTCGTTCTGCTCCATCATTCATCGGAGTTCACCCCCTCGATGAGAAGTATTATTCCTCCGAGGTCATAAAATGCAAGGATGGTTCTAGGTCTTTTACTATAGATCGTCTCAATGACAATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTACATCTGCCTGTGCAAGAGGGAAATTCTATTGTCGGAATATGGGAAGTACACCACGGTTTATTTTTTCTTCCCGTGTCAACGATCATATATGTGATTGCTGTGATGGAAGTGATGAATATGAGGGTAATATCTTTTGCCCGAACACATGTGTCATGGGTGGGAATATGTACAAGAGCAAAAATGATATTTCAACCACCCGAGATGTTGATATAGTCATCAGAAAAGTTAAGGAAGAAATCACCAAGGAGGACTTGTTCCAAAAGCTAACTGGTTTGAAGCTAGTAATAGTCCTACAGGTAGCTCTCACAAGTTTTGCAATCCTCATTTGGGCAAACCGTTGTCGTGTGAAATCTAAGAGAAGACGACACCGTTGA

Coding sequence (CDS)

ATGGAACCCCGCTCTCCAAAGTCTACCTCCTTCGCTTCACTGCTTTCCATATGTTTATTCTTCACGTCTGTTCGTTCTGCTCCATCATTCATCGGAGTTCACCCCCTCGATGAGAAGTATTATTCCTCCGAGGTCATAAAATGCAAGGATGGTTCTAGGTCTTTTACTATAGATCGTCTCAATGACAATTTCTGTGACTGCGTTGATGGAACCGATGAGCCTGGTACATCTGCCTGTGCAAGAGGGAAATTCTATTGTCGGAATATGGGAAGTACACCACGGTTTATTTTTTCTTCCCGTGTCAACGATCATATATGTGATTGCTGTGATGGAAGTGATGAATATGAGGGTAATATCTTTTGCCCGAACACATGTGTCATGGGTGGGAATATGTACAAGAGCAAAAATGATATTTCAACCACCCGAGATGTTGATATAGTCATCAGAAAAGTTAAGGAAGAAATCACCAAGGAGGACTTGTTCCAAAAGCTAACTGGTTTGAAGCTAGTAATAGTCCTACAGGTAGCTCTCACAAGTTTTGCAATCCTCATTTGGGCAAACCGTTGTCGTGTGAAATCTAAGAGAAGACGACACCGTTGA

Homology

[Close]

BLAST of CSPI07G03400 vs. Swiss-Prot
Match: PSL4_ARATH (Glucosidase 2 subunit beta OS=Arabidopsis thaliana GN=PSL4 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 7.9e-39
Identity = 86/162 (53.09%), Postives = 104/162 (64.20%), Query Frame = 1


Query: 10  SFASLLSICLFFTSVRSAPSFIGVHPLDEKYY-SSEVIKCKDGSRSFTIDRLNDNFCDCV 69
           SF  LL+  +  +S      F+G+ P DEKYY SS  IKCKDGS+ FT  +LND+FCDC 
Sbjct: 17  SFLLLLASAIRSSSSPPNDPFLGISPQDEKYYKSSSEIKCKDGSKKFTKAQLNDDFCDCS 76

Query: 70  DGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMG 129
           DGTDEPGTSAC  GKFYCRN G +P  +FSSRVND ICDCCDGSDEY+G++ C NTC   
Sbjct: 77  DGTDEPGTSACPTGKFYCRNAGHSPVILFSSRVNDGICDCCDGSDEYDGHVSCQNTCWEA 136

Query: 130 GNMYKS--KNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLK 169
           G   +   K  I T     +VIR+ + E  K  L +    LK
Sbjct: 137 GKAARENLKKKIETYNQ-GLVIRRQEIEQAKVGLEKDAAELK 177

[Close]

BLAST of CSPI07G03400 vs. Swiss-Prot
Match: GLU2B_ORYSI (Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica GN=OsI_01383 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.4e-35
Identity = 78/165 (47.27%), Postives = 101/165 (61.21%), Query Frame = 1


Query: 14  LLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDE 73
           LL I     + R     +G+ P DE Y+   VI+C+DGS  F  D+LND+FCDC DGTDE
Sbjct: 11  LLRISASAAASRPPLDTLGIPPQDEAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDE 70

Query: 74  PGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNMY- 133
           PGTSAC  GKFYC+N G +P  IFSSRVND ICDCCDGSDEY+ N+ C NTC   G    
Sbjct: 71  PGTSACPEGKFYCQNAGHSPITIFSSRVNDGICDCCDGSDEYDSNVTCKNTCWEAGKAAR 130

Query: 134 -KSKNDISTTRDVDIV----IRKVKEEITKEDL-FQKLTGLKLVI 172
            K K  ++T +   ++    I+K K    K++    KL G + ++
Sbjct: 131 DKLKKKVATYKSGVVIRNQEIQKAKVAFAKDEAELAKLKGEEKIL 175

[Close]

BLAST of CSPI07G03400 vs. Swiss-Prot
Match: GLU2B_ORYSJ (Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica GN=Os01g0276800 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.4e-35
Identity = 78/165 (47.27%), Postives = 101/165 (61.21%), Query Frame = 1


Query: 14  LLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDE 73
           LL I     + R     +G+ P DE Y+   VI+C+DGS  F  D+LND+FCDC DGTDE
Sbjct: 11  LLRISASAAASRPPLDTLGIPPQDEAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDE 70

Query: 74  PGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNMY- 133
           PGTSAC  GKFYC+N G +P  IFSSRVND ICDCCDGSDEY+ N+ C NTC   G    
Sbjct: 71  PGTSACPEGKFYCQNAGHSPITIFSSRVNDGICDCCDGSDEYDSNVTCKNTCWEAGKAAR 130

Query: 134 -KSKNDISTTRDVDIV----IRKVKEEITKEDL-FQKLTGLKLVI 172
            K K  ++T +   ++    I+K K    K++    KL G + ++
Sbjct: 131 DKLKKKVATYKSGVVIRNQEIQKAKVAFAKDEAELAKLKGEEKIL 175

[Close]

BLAST of CSPI07G03400 vs. Swiss-Prot
Match: GLU2B_MOUSE (Glucosidase 2 subunit beta OS=Mus musculus GN=Prkcsh PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.9e-25
Identity = 48/94 (51.06%), Postives = 61/94 (64.89%), Query Frame = 1


Query: 40  YYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSS 99
           Y  S+   C DG+ +   D++ND++CDC DG+DEPGT+AC  G F+C N G  P +I SS
Sbjct: 31  YEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSS 90

Query: 100 RVNDHICDCCDGSDEYEGNIFCPNTCVMGGNMYK 134
           RVND +CDCCDG+DEY     C NTC   G   K
Sbjct: 91  RVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEK 124

[Close]

BLAST of CSPI07G03400 vs. Swiss-Prot
Match: GLU2B_HUMAN (Glucosidase 2 subunit beta OS=Homo sapiens GN=PRKCSH PE=1 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 5.0e-25
Identity = 46/86 (53.49%), Postives = 60/86 (69.77%), Query Frame = 1


Query: 40  YYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSS 99
           Y  S+   C DGS +   D++ND++CDC DG+DEPGT+AC  G F+C N G  P +I S+
Sbjct: 31  YDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSN 90

Query: 100 RVNDHICDCCDGSDEYEGNIFCPNTC 126
           RVND +CDCCDG+DEY   + C NTC
Sbjct: 91  RVNDGVCDCCDGTDEYNSGVICENTC 116

[Close]

BLAST of CSPI07G03400 vs. TrEMBL
Match: A0A0A0K1G3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G041350 PE=4 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 3.2e-111
Identity = 198/199 (99.50%), Postives = 199/199 (100.00%), Query Frame = 1


Query: 1   MEPRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRL 60
           MEPRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRL
Sbjct: 1   MEPRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRL 60

Query: 61  NDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF 120
           NDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF
Sbjct: 61  NDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF 120

Query: 121 CPNTCVMGGNMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSF 180
           CPNTCVMGGNMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVI+LQVALTSF
Sbjct: 121 CPNTCVMGGNMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTSF 180

Query: 181 AILIWANRCRVKSKRRRHR 200
           AILIWANRCRVKSKRRRHR
Sbjct: 181 AILIWANRCRVKSKRRRHR 199

[Close]

BLAST of CSPI07G03400 vs. TrEMBL
Match: V4W239_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016674mg PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 3.0e-61
Identity = 116/188 (61.70%), Postives = 143/188 (76.06%), Query Frame = 1


Query: 14  LLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDE 73
           L+S+C     V+   S +GVHPLDEKY+S EVIKCKDGS+SFT DRLNDNFCDC+DGTDE
Sbjct: 12  LISLCFLVVFVQCKSSLLGVHPLDEKYFSKEVIKCKDGSKSFTRDRLNDNFCDCIDGTDE 71

Query: 74  PGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNM-Y 133
           PGTSAC  GKFYC N+GSTP+FIFSSRVND ICDCCDGSDEY+ +I CPNTCVMGGN+ Y
Sbjct: 72  PGTSACPAGKFYCGNVGSTPQFIFSSRVNDRICDCCDGSDEYDSSIKCPNTCVMGGNIEY 131

Query: 134 KSKNDISTTRDV-DIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSFAILIWANRCRV 193
           K+++ IST  D   I  R  K  + KEDL ++L  +K+V++LQ  +  F +++W    RV
Sbjct: 132 KAQSYISTINDAGSIDARGAKIPVNKEDLIERLKDVKMVVILQSFVIIFLVVLWIMHHRV 191

Query: 194 KSKRRRHR 200
           +SKRR  R
Sbjct: 192 RSKRRHSR 199

[Close]

BLAST of CSPI07G03400 vs. TrEMBL
Match: A0A0D2PRF3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G097900 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 5.1e-61
Identity = 114/186 (61.29%), Postives = 144/186 (77.42%), Query Frame = 1


Query: 16  SICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDEPG 75
           ++  F   V S+ S +G+HPLD KY+++EVIKCKDGS+SFT DRLNDNFCDC+DGTDEPG
Sbjct: 19  AVSFFLLLVSSSKSPLGIHPLDAKYFAAEVIKCKDGSKSFTRDRLNDNFCDCLDGTDEPG 78

Query: 76  TSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNM-YKS 135
           TSAC  GKFYCRN+GS P+FIFSSRVNDH CDCCDGSDEY+G+I CPNTC+MGGN+ YK+
Sbjct: 79  TSACPAGKFYCRNVGSIPQFIFSSRVNDHFCDCCDGSDEYDGSILCPNTCIMGGNVEYKT 138

Query: 136 KNDISTTRDV-DIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSFAILIWANRCRVKS 195
           +  +STT  +    ++++K  I  EDL Q L GLK++ V +VAL  F ++ W    RVKS
Sbjct: 139 EEYVSTTTHLHSTKLKEMKIGIKLEDLIQTLAGLKMITVAEVALGCFLVVKWVFHKRVKS 198

Query: 196 KRRRHR 200
           K+R HR
Sbjct: 199 KKRHHR 204

[Close]

BLAST of CSPI07G03400 vs. TrEMBL
Match: A0A0B0MJA2_GOSAR (Glucosidase 2 subunit beta OS=Gossypium arboreum GN=F383_23982 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.6e-59
Identity = 112/186 (60.22%), Postives = 143/186 (76.88%), Query Frame = 1


Query: 16  SICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDEPG 75
           ++  F   V S+ S +G+HPLD KY+++EVIKCKDG +SFT DRLNDNFCDC+DGTDEPG
Sbjct: 19  AVSFFLLLVSSSKSPLGIHPLDAKYFAAEVIKCKDGFKSFTRDRLNDNFCDCLDGTDEPG 78

Query: 76  TSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNM-YKS 135
           TSAC  GKFYCRN+GS P+FIFSSRVNDH CDCCDGSDEY+G+I CPNTC+MGGN+ YK+
Sbjct: 79  TSACPAGKFYCRNVGSIPQFIFSSRVNDHFCDCCDGSDEYDGSILCPNTCIMGGNVEYKT 138

Query: 136 KNDISTTRDV-DIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSFAILIWANRCRVKS 195
           ++ +STT  +    ++++K  I  EDL Q L GLK++ V +VAL    ++ W    RVKS
Sbjct: 139 EDYVSTTSHLHSTKLKEMKIGIKLEDLIQTLAGLKMITVAEVALGCVLVVKWIFHKRVKS 198

Query: 196 KRRRHR 200
           K+R HR
Sbjct: 199 KKRHHR 204

[Close]

BLAST of CSPI07G03400 vs. TrEMBL
Match: V4U799_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016674mg PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 3.7e-59
Identity = 120/203 (59.11%), Postives = 145/203 (71.43%), Query Frame = 1


Query: 14  LLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDE 73
           L+S+C     V+   S +GVHPLDEKY+S EVIKCKDGS+SFT DRLNDNFCDC+DGTDE
Sbjct: 12  LISLCFLVVFVQCKSSLLGVHPLDEKYFSKEVIKCKDGSKSFTRDRLNDNFCDCIDGTDE 71

Query: 74  PGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNM-Y 133
           PGTSAC  GKFYC N+GSTP+FIFSSRVND ICDCCDGSDEY+ +I CPNTCVMGGN+ Y
Sbjct: 72  PGTSACPAGKFYCGNVGSTPQFIFSSRVNDRICDCCDGSDEYDSSIKCPNTCVMGGNIEY 131

Query: 134 KSKNDISTTRDV-DIVIRKVKEEITKEDLFQKLTGLKLVIVLQ-----------VALTSF 193
           K+++ IST  D   I  R  K  + KEDL ++L  +K+V++LQ           V L SF
Sbjct: 132 KAQSYISTINDAGSIDARGAKIPVNKEDLIERLKDVKMVVILQSSLVFADVKMVVILQSF 191

Query: 194 AIL----IWANRCRVKSKRRRHR 200
            I+    +W    RV+SKRR  R
Sbjct: 192 VIIFLVVLWIMHHRVRSKRRHSR 214

[Close]

BLAST of CSPI07G03400 vs. TAIR10
Match: AT2G42390.1 (AT2G42390.1 protein kinase C substrate, heavy chain-related)

HSP 1 Score: 224.9 bits (572), Expect = 4.3e-59
Identity = 107/189 (56.61%), Postives = 136/189 (71.96%), Query Frame = 1


Query: 16  SICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDEPG 75
           S+ +   SV S    +GVHPLDEKY+ S+VIKCKDGS+SFT DRLNDNFCDC+DGTDEPG
Sbjct: 11  SLAVVVISVASTSPLVGVHPLDEKYFDSDVIKCKDGSKSFTRDRLNDNFCDCLDGTDEPG 70

Query: 76  TSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGN---MY 135
           TSAC  GKFYCRN+GS+P+F++SSRVND ICDCCDGSDEYE +I CPNTC+MGGN   +Y
Sbjct: 71  TSACPNGKFYCRNIGSSPKFVYSSRVNDRICDCCDGSDEYESSIHCPNTCIMGGNVNYIY 130

Query: 136 KSKNDISTTRDVDIVIRKVKEEIT---KEDLFQKLTGLKLVIVLQVALTSFAILIWANRC 195
           K + ++ +           KE  T    +D+ + L G+KLV  LQ+    F +++W  R 
Sbjct: 131 KPRANLKSIHLQLGSTPHPKEFYTIGNLQDMVKNLQGMKLVFALQMVFIGFLVILWMLRS 190

Query: 196 RVKSKRRRH 199
           R +SKRRR+
Sbjct: 191 RARSKRRRY 199

[Close]

BLAST of CSPI07G03400 vs. TAIR10
Match: AT5G56360.1 (AT5G56360.1 calmodulin-binding protein)

HSP 1 Score: 161.8 bits (408), Expect = 4.5e-40
Identity = 86/162 (53.09%), Postives = 104/162 (64.20%), Query Frame = 1


Query: 10  SFASLLSICLFFTSVRSAPSFIGVHPLDEKYY-SSEVIKCKDGSRSFTIDRLNDNFCDCV 69
           SF  LL+  +  +S      F+G+ P DEKYY SS  IKCKDGS+ FT  +LND+FCDC 
Sbjct: 17  SFLLLLASAIRSSSSPPNDPFLGISPQDEKYYKSSSEIKCKDGSKKFTKAQLNDDFCDCS 76

Query: 70  DGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMG 129
           DGTDEPGTSAC  GKFYCRN G +P  +FSSRVND ICDCCDGSDEY+G++ C NTC   
Sbjct: 77  DGTDEPGTSACPTGKFYCRNAGHSPVILFSSRVNDGICDCCDGSDEYDGHVSCQNTCWEA 136

Query: 130 GNMYKS--KNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLK 169
           G   +   K  I T     +VIR+ + E  K  L +    LK
Sbjct: 137 GKAARENLKKKIETYNQ-GLVIRRQEIEQAKVGLEKDAAELK 177

[Close]

BLAST of CSPI07G03400 vs. NCBI nr
Match: gi|449453541|ref|XP_004144515.1| (PREDICTED: glucosidase 2 subunit beta [Cucumis sativus])

HSP 1 Score: 409.1 bits (1050), Expect = 4.5e-111
Identity = 198/199 (99.50%), Postives = 199/199 (100.00%), Query Frame = 1


Query: 1   MEPRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRL 60
           MEPRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRL
Sbjct: 1   MEPRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRL 60

Query: 61  NDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF 120
           NDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF
Sbjct: 61  NDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF 120

Query: 121 CPNTCVMGGNMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSF 180
           CPNTCVMGGNMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVI+LQVALTSF
Sbjct: 121 CPNTCVMGGNMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTSF 180

Query: 181 AILIWANRCRVKSKRRRHR 200
           AILIWANRCRVKSKRRRHR
Sbjct: 181 AILIWANRCRVKSKRRRHR 199

[Close]

BLAST of CSPI07G03400 vs. NCBI nr
Match: gi|659110908|ref|XP_008455474.1| (PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo])

HSP 1 Score: 396.0 bits (1016), Expect = 4.0e-107
Identity = 190/199 (95.48%), Postives = 195/199 (97.99%), Query Frame = 1


Query: 1   MEPRSPKSTSFASLLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRL 60
           ME RSPKSTSF SLLSICLFFTSVR+APSF+GVHPLDEKYYSSEVIKCKDGSRSFTIDRL
Sbjct: 1   MEARSPKSTSFVSLLSICLFFTSVRAAPSFLGVHPLDEKYYSSEVIKCKDGSRSFTIDRL 60

Query: 61  NDNFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF 120
           ND+FCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF
Sbjct: 61  NDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIF 120

Query: 121 CPNTCVMGGNMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSF 180
           CPNTCVMGGNMYKS ND+STTRDVDIVIRKVKEEITKEDLFQKLTGLKLVI+LQVALT F
Sbjct: 121 CPNTCVMGGNMYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTIF 180

Query: 181 AILIWANRCRVKSKRRRHR 200
           AILIWANRCRVKSKRRRHR
Sbjct: 181 AILIWANRCRVKSKRRRHR 199

[Close]

BLAST of CSPI07G03400 vs. NCBI nr
Match: gi|659110912|ref|XP_008455476.1| (PREDICTED: glucosidase 2 subunit beta isoform X2 [Cucumis melo])

HSP 1 Score: 253.4 bits (646), Expect = 3.2e-64
Identity = 122/130 (93.85%), Postives = 124/130 (95.38%), Query Frame = 1


Query: 70  GTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGG 129
           G    GTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGG
Sbjct: 32  GVHPLGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGG 91

Query: 130 NMYKSKNDISTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSFAILIWANRC 189
           NMYKS ND+STTRDVDIVIRKVKEEITKEDLFQKLTGLKLVI+LQVALT FAILIWANRC
Sbjct: 92  NMYKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALTIFAILIWANRC 151

Query: 190 RVKSKRRRHR 200
           RVKSKRRRHR
Sbjct: 152 RVKSKRRRHR 161

[Close]

BLAST of CSPI07G03400 vs. NCBI nr
Match: gi|568829492|ref|XP_006469056.1| (PREDICTED: glucosidase 2 subunit beta isoform X2 [Citrus sinensis])

HSP 1 Score: 246.9 bits (629), Expect = 3.0e-62
Identity = 118/188 (62.77%), Postives = 144/188 (76.60%), Query Frame = 1


Query: 14  LLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDE 73
           L+S+C     V+   S +GVHPLDEKY+S EVIKCKDGS+SFT DRLNDNFCDC+DGTDE
Sbjct: 12  LMSLCFLVVFVQCKSSLLGVHPLDEKYFSKEVIKCKDGSKSFTRDRLNDNFCDCIDGTDE 71

Query: 74  PGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNM-Y 133
           PGTSAC  GKFYC N+GSTPRFIFSSRVND ICDCCDGSDEY+ +I CPNTCVMGGN+ Y
Sbjct: 72  PGTSACPAGKFYCGNVGSTPRFIFSSRVNDRICDCCDGSDEYDSSIKCPNTCVMGGNIEY 131

Query: 134 KSKNDISTTRDV-DIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSFAILIWANRCRV 193
           K+++ IST  D   I  R  K  + KEDL ++L  +K+V+VLQ+ +  F +++W    RV
Sbjct: 132 KAQSYISTINDAGSIDARGAKIPVNKEDLIERLKDVKMVVVLQLFVIIFLVVLWITHHRV 191

Query: 194 KSKRRRHR 200
           +SKRR  R
Sbjct: 192 RSKRRHSR 199

[Close]

BLAST of CSPI07G03400 vs. NCBI nr
Match: gi|567908851|ref|XP_006446739.1| (hypothetical protein CICLE_v10016674mg [Citrus clementina])

HSP 1 Score: 243.0 bits (619), Expect = 4.3e-61
Identity = 116/188 (61.70%), Postives = 143/188 (76.06%), Query Frame = 1


Query: 14  LLSICLFFTSVRSAPSFIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDNFCDCVDGTDE 73
           L+S+C     V+   S +GVHPLDEKY+S EVIKCKDGS+SFT DRLNDNFCDC+DGTDE
Sbjct: 12  LISLCFLVVFVQCKSSLLGVHPLDEKYFSKEVIKCKDGSKSFTRDRLNDNFCDCIDGTDE 71

Query: 74  PGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNIFCPNTCVMGGNM-Y 133
           PGTSAC  GKFYC N+GSTP+FIFSSRVND ICDCCDGSDEY+ +I CPNTCVMGGN+ Y
Sbjct: 72  PGTSACPAGKFYCGNVGSTPQFIFSSRVNDRICDCCDGSDEYDSSIKCPNTCVMGGNIEY 131

Query: 134 KSKNDISTTRDV-DIVIRKVKEEITKEDLFQKLTGLKLVIVLQVALTSFAILIWANRCRV 193
           K+++ IST  D   I  R  K  + KEDL ++L  +K+V++LQ  +  F +++W    RV
Sbjct: 132 KAQSYISTINDAGSIDARGAKIPVNKEDLIERLKDVKMVVILQSFVIIFLVVLWIMHHRV 191

Query: 194 KSKRRRHR 200
           +SKRR  R
Sbjct: 192 RSKRRHSR 199

The following BLAST results are available for this feature:

BLAST of CSPI07G03400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PSL4_ARATH	7.9e-39	53.09	Glucosidase 2 subunit beta OS=Arabidopsis thaliana GN=PSL4 PE=2 SV=1	[more]
GLU2B_ORYSI	1.4e-35	47.27	Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica GN=OsI_01383 PE=3 SV=1	[more]
GLU2B_ORYSJ	1.4e-35	47.27	Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica GN=Os01g0276800 PE=2 ...	[more]
GLU2B_MOUSE	2.9e-25	51.06	Glucosidase 2 subunit beta OS=Mus musculus GN=Prkcsh PE=1 SV=1	[more]
GLU2B_HUMAN	5.0e-25	53.49	Glucosidase 2 subunit beta OS=Homo sapiens GN=PRKCSH PE=1 SV=2	[more]

BLAST of CSPI07G03400 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K1G3_CUCSA	3.2e-111	99.50	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G041350 PE=4 SV=1	[more]
V4W239_9ROSI	3.0e-61	61.70	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016674mg PE=4 SV=1	[more]
A0A0D2PRF3_GOSRA	5.1e-61	61.29	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G097900 PE=4 SV=1	[more]
A0A0B0MJA2_GOSAR	1.6e-59	60.22	Glucosidase 2 subunit beta OS=Gossypium arboreum GN=F383_23982 PE=4 SV=1	[more]
V4U799_9ROSI	3.7e-59	59.11	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016674mg PE=4 SV=1	[more]

BLAST of CSPI07G03400 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G42390.1	4.3e-59	56.61	protein kinase C substrate, heavy chain-related	[more]
AT5G56360.1	4.5e-40	53.09	calmodulin-binding protein	[more]

BLAST of CSPI07G03400 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449453541\|ref\|XP_004144515.1\|	4.5e-111	99.50	PREDICTED: glucosidase 2 subunit beta [Cucumis sativus]	[more]
gi\|659110908\|ref\|XP_008455474.1\|	4.0e-107	95.48	PREDICTED: glucosidase 2 subunit beta isoform X1 [Cucumis melo]	[more]
gi\|659110912\|ref\|XP_008455476.1\|	3.2e-64	93.85	PREDICTED: glucosidase 2 subunit beta isoform X2 [Cucumis melo]	[more]
gi\|568829492\|ref\|XP_006469056.1\|	3.0e-62	62.77	PREDICTED: glucosidase 2 subunit beta isoform X2 [Citrus sinensis]	[more]
gi\|567908851\|ref\|XP_006446739.1\|	4.3e-61	61.70	hypothetical protein CICLE_v10016674mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002172	Low-density lipoprotein (LDL) receptor class A repeat
IPR028146	PRKCSH_N
IPR002172	Low-density lipoprotein (LDL) receptor class A repeat
IPR028146	PRKCSH_N

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0005515	protein binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0006491	N-glycan processing
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G03400.1	CSPI07G03400.1	mRNA
CSPI07G03400.2	CSPI07G03400.2	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002172	Low-density lipoprotein (LDL) receptor class A repeat	GENE3D	G3DSA:4.10.400.10		coord: 78..114 score: 3.
IPR028146	Glucosidase II beta subunit, N-terminal	PFAM	PF12999	PRKCSH-like	coord: 13..148 score: 2.4
None	No IPR available	PANTHER	PTHR12630	N-LINKED OLIGOSACCHARIDE PROCESSING	coord: 1..188 score: 3.9
None	No IPR available	PANTHER	PTHR12630:SF9	SUBFAMILY NOT NAMED	coord: 1..188 score: 3.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI07G03400	Cla015628	Watermelon (97103) v1	cpiwmB624
CSPI07G03400	Csa7G041350	Cucumber (Chinese Long) v2	cpicuB354
CSPI07G03400	MELO3C018846	Melon (DHL92) v3.5.1	cpimeB538
CSPI07G03400	ClCG02G002220	Watermelon (Charleston Gray)	cpiwcgB574
CSPI07G03400	Lsi11G014200	Bottle gourd (USVL1VR-Ls)	cpilsiB505
CSPI07G03400	MELO3C018846.2	Melon (DHL92) v3.6.1	cpimedB523
CSPI07G03400	CsaV3_7G003280	Cucumber (Chinese Long) v3	cpicucB415
CSPI07G03400	Cla97C02G028620	Watermelon (97103) v2	cpiwmbB553
CSPI07G03400	Bhi05G001212	Wax gourd	cpiwgoB681
CSPI07G03400	Cucsa.148850	Cucumber (Gy14) v1	cgycpiB220
CSPI07G03400	CmaCh19G009020	Cucurbita maxima (Rimu)	cmacpiB533
CSPI07G03400	CmoCh19G009250	Cucurbita moschata (Rifu)	cmocpiB524
CSPI07G03400	Cp4.1LG15g07190	Cucurbita pepo (Zucchini)	cpecpiB257
CSPI07G03400	CsGy7G003060	Cucumber (Gy14) v2	cgybcpiB332
CSPI07G03400	Carg04182	Silver-seed gourd	carcpiB0235

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI07G03400	Cucurbita maxima (Rimu)	cmacpiB144
CSPI07G03400	Cucurbita maxima (Rimu)	cmacpiB576
CSPI07G03400	Cucurbita moschata (Rifu)	cmocpiB131
CSPI07G03400	Cucurbita moschata (Rifu)	cmocpiB568
CSPI07G03400	Cucumber (Chinese Long) v2	cpicuB326
CSPI07G03400	Cucumber (Chinese Long) v2	cpicuB358
CSPI07G03400	Melon (DHL92) v3.5.1	cpimeB535
CSPI07G03400	Melon (DHL92) v3.5.1	cpimeB520
CSPI07G03400	Watermelon (97103) v1	cpiwmB617
CSPI07G03400	Watermelon (97103) v1	cpiwmB621
CSPI07G03400	Cucurbita pepo (Zucchini)	cpecpiB301
CSPI07G03400	Cucurbita pepo (Zucchini)	cpecpiB692
CSPI07G03400	Bottle gourd (USVL1VR-Ls)	cpilsiB514
CSPI07G03400	Melon (DHL92) v3.6.1	cpimedB508
CSPI07G03400	Melon (DHL92) v3.6.1	cpimedB521
CSPI07G03400	Cucumber (Gy14) v2	cgybcpiB087
CSPI07G03400	Silver-seed gourd	carcpiB0335
CSPI07G03400	Cucumber (Chinese Long) v3	cpicucB385
CSPI07G03400	Cucumber (Chinese Long) v3	cpicucB419
CSPI07G03400	Watermelon (97103) v2	cpiwmbB552
CSPI07G03400	Wax gourd	cpiwgoB680
CSPI07G03400	Wax gourd	cpiwgoB691
CSPI07G03400	Wild cucumber (PI 183967)	cpicpiB090