CSPI05G16500 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G16500
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cystatin
Location	Chr5 : 17792568 .. 17793101 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAACGCCAACACTATAAATATGGAACCCGTTCCCATGCATATTATATTCAAAAGCAATTAAACATAGTCTGTTCCAAAGTATACATATCTGTCGTGGAAAGGTGTTAGCAATGTGTTCTAGGAATTTTGATGTTCCTCCAGTGCCAATTAAGGATATTGATTCTGCAGAAATTCAAGGATATGCAAGGTTTGCAACAGAAAGGCATAATGAAATTACAGGTGACAAACTGCAGTTCCAGAGGGCAATAAATGGGTTGGAACAGGGACGAAGGTTGGGTCAAATATGTTTCACGCTTGTGTTGGAAGCTATAAACTCTGGTGGATTTCTTTGGACTTATACAGCTATAGTATTTTACAATCAACGGGCAGCGTTACAACTCATGGAATTTGGACCTGTTCTTCCTGAGCATTTACAACACTAAACTAGCTGCTACTTAACAATATACAACCACATATATATACCTATACATATATACCTACATATATATATATATATAAATAAAATAGAATTATGATACTTACCTTTGTTTCTTG

mRNA sequence

ATGTGTTCTAGGAATTTTGATGTTCCTCCAGTGCCAATTAAGGATATTGATTCTGCAGAAATTCAAGGATATGCAAGGTTTGCAACAGAAAGGCATAATGAAATTACAGGTGACAAACTGCAGTTCCAGAGGGCAATAAATGGGTTGGAACAGGGACGAAGGTTGGGTCAAATATGTTTCACGCTTGTGTTGGAAGCTATAAACTCTGGTGGATTTCTTTGGACTTATACAGCTATAGTATTTTACAATCAACGGGCAGCGTTACAACTCATGGAATTTGGACCTGTTCTTCCTGAGCATTTACAACACTAA

Coding sequence (CDS)

ATGTGTTCTAGGAATTTTGATGTTCCTCCAGTGCCAATTAAGGATATTGATTCTGCAGAAATTCAAGGATATGCAAGGTTTGCAACAGAAAGGCATAATGAAATTACAGGTGACAAACTGCAGTTCCAGAGGGCAATAAATGGGTTGGAACAGGGACGAAGGTTGGGTCAAATATGTTTCACGCTTGTGTTGGAAGCTATAAACTCTGGTGGATTTCTTTGGACTTATACAGCTATAGTATTTTACAATCAACGGGCAGCGTTACAACTCATGGAATTTGGACCTGTTCTTCCTGAGCATTTACAACACTAA

Homology

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BLAST of CSPI05G16500 vs. TrEMBL
Match: A0A0A0KNH4_CUCSA (Cystatin OS=Cucumis sativus GN=Csa_5G485690 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.1e-22
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 1


Query: 1  MCSRNFDVPPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR 54
          MCSRNFDVPPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR
Sbjct: 1  MCSRNFDVPPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR 53

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BLAST of CSPI05G16500 vs. TrEMBL
Match: A0A0A0KRZ8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G487690 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 7.1e-22
Identity = 54/92 (58.70%), Postives = 66/92 (71.74%), Query Frame = 1


Query: 9   PPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGRRLGQICFTLVLEAIN 68
           P VPIKD+DS EI+G+  FA   HNE TG+ L+F + INGLEQGRR  +I FTLVL A N
Sbjct: 13  PAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSAKN 72

Query: 69  SGGFLWTYTAIVFYNQRAALQLMEFGPVLPEH 101
           S G +WTY   VF NQRA ++L+EF  VL E+
Sbjct: 73  SIGIIWTYKVKVFLNQRAGVRLLEFEAVLNEN 104

[Close]

BLAST of CSPI05G16500 vs. TrEMBL
Match: A0A0A0KNL2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G487700 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.5e-19
Identity = 54/95 (56.84%), Postives = 65/95 (68.42%), Query Frame = 1


Query: 7   DVPPVPIKDIDSAEIQGYARFATERHNEI-TGDKLQFQRAINGLEQGRRLGQICFTLVLE 66
           D   VPIKD+DS EIQG    A   HNE  TG+ L+F + INGLE+GRR  QI +TLVL 
Sbjct: 17  DYDTVPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVYTLVLS 76

Query: 67  AINSGGFLWTYTAIVFYNQRAALQLMEFGPVLPEH 101
           A NS G +WTY A V +N RAA++L EF  VLPE+
Sbjct: 77  AKNSLGIVWTYKAKVIFNNRAAVRLPEFEAVLPEN 111

[Close]

BLAST of CSPI05G16500 vs. NCBI nr
Match: gi|700196013|gb|KGN51190.1| (Cystatin [Cucumis sativus])

HSP 1 Score: 114.0 bits (284), Expect = 1.6e-22
Identity = 53/53 (100.00%), Postives = 53/53 (100.00%), Query Frame = 1


Query: 1  MCSRNFDVPPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR 54
          MCSRNFDVPPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR
Sbjct: 1  MCSRNFDVPPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGR 53

[Close]

BLAST of CSPI05G16500 vs. NCBI nr
Match: gi|700196015|gb|KGN51192.1| (hypothetical protein Csa_5G487690 [Cucumis sativus])

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-21
Identity = 54/92 (58.70%), Postives = 66/92 (71.74%), Query Frame = 1


Query: 9   PPVPIKDIDSAEIQGYARFATERHNEITGDKLQFQRAINGLEQGRRLGQICFTLVLEAIN 68
           P VPIKD+DS EI+G+  FA   HNE TG+ L+F + INGLEQGRR  +I FTLVL A N
Sbjct: 13  PAVPIKDVDSEEIRGFGEFAVSEHNEATGENLEFVKVINGLEQGRRGFRIDFTLVLSAKN 72

Query: 69  SGGFLWTYTAIVFYNQRAALQLMEFGPVLPEH 101
           S G +WTY   VF NQRA ++L+EF  VL E+
Sbjct: 73  SIGIIWTYKVKVFLNQRAGVRLLEFEAVLNEN 104

[Close]

BLAST of CSPI05G16500 vs. NCBI nr
Match: gi|700196016|gb|KGN51193.1| (hypothetical protein Csa_5G487700 [Cucumis sativus])

HSP 1 Score: 103.6 bits (257), Expect = 2.1e-19
Identity = 54/95 (56.84%), Postives = 65/95 (68.42%), Query Frame = 1


Query: 7   DVPPVPIKDIDSAEIQGYARFATERHNEI-TGDKLQFQRAINGLEQGRRLGQICFTLVLE 66
           D   VPIKD+DS EIQG    A   HNE  TG+ L+F + INGLE+GRR  QI +TLVL 
Sbjct: 17  DYDTVPIKDVDSEEIQGIGGMAVRMHNETSTGENLEFVKVINGLEKGRRGFQIVYTLVLS 76

Query: 67  AINSGGFLWTYTAIVFYNQRAALQLMEFGPVLPEH 101
           A NS G +WTY A V +N RAA++L EF  VLPE+
Sbjct: 77  AKNSLGIVWTYKAKVIFNNRAAVRLPEFEAVLPEN 111

The following BLAST results are available for this feature:

BLAST of CSPI05G16500 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI05G16500 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNH4_CUCSA	1.1e-22	100.00	Cystatin OS=Cucumis sativus GN=Csa_5G485690 PE=4 SV=1	[more]
A0A0A0KRZ8_CUCSA	7.1e-22	58.70	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G487690 PE=4 SV=1	[more]
A0A0A0KNL2_CUCSA	1.5e-19	56.84	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G487700 PE=4 SV=1	[more]

BLAST of CSPI05G16500 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CSPI05G16500 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700196013\|gb\|KGN51190.1\|	1.6e-22	100.00	Cystatin [Cucumis sativus]	[more]
gi\|700196015\|gb\|KGN51192.1\|	1.0e-21	58.70	hypothetical protein Csa_5G487690 [Cucumis sativus]	[more]
gi\|700196016\|gb\|KGN51193.1\|	2.1e-19	56.84	hypothetical protein Csa_5G487700 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G16500.1	CSPI05G16500.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PRODOM	PD001231		coord: 18..81 score: 3.
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 13..92 score: 7.
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 13..51 score: 6.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 13..97 score: 2.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI05G16500	Cla003258	Watermelon (97103) v1	cpiwmB369
CSPI05G16500	Csa5G485690	Cucumber (Chinese Long) v2	cpicuB239
CSPI05G16500	ClCG09G020790	Watermelon (Charleston Gray)	cpiwcgB355
CSPI05G16500	Lsi02G017400	Bottle gourd (USVL1VR-Ls)	cpilsiB351
CSPI05G16500	CsaV3_5G025520	Cucumber (Chinese Long) v3	cpicucB284
CSPI05G16500	Cla97C09G182210	Watermelon (97103) v2	cpiwmbB428
CSPI05G16500	Bhi06G000662	Wax gourd	cpiwgoB475
CSPI05G16500	Cucsa.275310	Cucumber (Gy14) v1	cgycpiB415
CSPI05G16500	CsGy5G016200	Cucumber (Gy14) v2	cgybcpiB225

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G16500	Cucurbita maxima (Rimu)	cmacpiB192
CSPI05G16500	Cucurbita maxima (Rimu)	cmacpiB763
CSPI05G16500	Cucurbita maxima (Rimu)	cmacpiB813
CSPI05G16500	Cucurbita moschata (Rifu)	cmocpiB175
CSPI05G16500	Cucurbita moschata (Rifu)	cmocpiB798
CSPI05G16500	Melon (DHL92) v3.5.1	cpimeB350
CSPI05G16500	Cucurbita pepo (Zucchini)	cpecpiB115
CSPI05G16500	Cucurbita pepo (Zucchini)	cpecpiB445
CSPI05G16500	Cucurbita pepo (Zucchini)	cpecpiB836
CSPI05G16500	Melon (DHL92) v3.6.1	cpimedB340
CSPI05G16500	Silver-seed gourd	carcpiB1100
CSPI05G16500	Watermelon (97103) v2	cpiwmbB430