CSPI04G11360 (gene) Wild cucumber (PI 183967)

NameCSPI04G11360
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRibonuclease 3-like protein 2
LocationChr4 : 9622654 .. 9624560 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCTCCGTCATTGCCCTTGAGCGCATTCTTTCCTATAACTTTAACAACAAGAGTCTCTTAGAAGAAGCCCTCACTCACCCCTCTTACTCTGCCTCTGCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGCTTCTCCTCACCTCCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATTCACCTGCTATGGATGTTAAGGTCTTCAAATTAACCTTCACTTCTCCTTTGTTCCTAAAAATCTTCTTTTTTTTTTCTTTTTTTTTTTTTTGCCCTGTTTTAACTTCATGTCTGTATTTTTTTTATTGTTCAGGTTAAAGAGTTTGTTGATTCTGTTGACCTAGAAGGTAGCTCCGTTCCTTATGGTAGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGGTAAGTTCTTAGTTACATTTGAAGTATGACTATTGTTACTTTATTAGCTGGGCACCAATTAGTTTTCTTTTCGTCTTCAATCTTTTGTAGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAGTTTGCCAAAGAAATGGAAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAATAGCATCGCGAGTGTCTATGTTGATGGTAAATTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATTAAAACATTTGTAACTATTGATGGTATCGATGAATCGTTTGAGATTGAAGGGGCAAAGCAGAAGCTACATGATGTTTGTTCCAAGAGAAAGTGGCAGAAACCAAATTACAGGTATATATTTTTATTGTATGATATTTGAGATGTTGCATACTTGCATTGTTTTGGACTCTGAATTAGGAGGTAGTTTTTATGTCGTAGTTGTCTCTTTTTCATTTAAAAGCATGCACCTTCACTTATATATTTGCTTTCTAACCAAGTAGGATGCTGAATTTTTCAGTAATTTATAATGAAATGCATTTTTTTTCTCTAAAGTCTGCCTGCTTCAATTGTAACCTTGTATCTTCATCGAACTATTTTCATTCTTTCTGTTCATTGTAAAAGTTGCAGCATTTTTTAAATTTAAGATTCATTTCACTGATCAATGCTCACTTATTTTAGAGAAATTAGTTTCAGCAACGACAGTGACCTTCTCAATTAATTTTAATGTTATTATATCTGTACACTTATTTGTTTAAAACTTATTTTCTTTTGAATCTCCACCCTCTGTGCATACCTTATCAACTAAAGGCCTGTTTCCCTTCCTTTTCCATCTTACACATTGATTTTTTATGTGAATAGAACTCTTCGATATCTGTGGGGATTATCTCGAGTCGTTTTGTGCGGGTCATAAACAAAAGATAAAACTCAGAGAGATCATTTACATACCATGAAGTTATTTGACTATCTTTTTCCCTTCTCCTTGTCCTCCTTTTTCAAACGTGTTCTAGTGCTAAATACTTTATGAATTTCTTACTTGCAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGATATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAAGATGCAGAGAACTCAGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCCTCTCTAA

mRNA sequence

ATGGAATCCTCCGTCATTGCCCTTGAGCGCATTCTTTCCTATAACTTTAACAACAAGAGTCTCTTAGAAGAAGCCCTCACTCACCCCTCTTACTCTGCCTCTGCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGCTTCTCCTCACCTCCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATTCACCTGCTATGGATGTTAAGGTTAAAGAGTTTGTTGATTCTGTTGACCTAGAAGGTAGCTCCGTTCCTTATGGTAGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAGTTTGCCAAAGAAATGGAAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAATAGCATCGCGAGTGTCTATGTTGATGGTAAATTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATTAAAACATTTGTAACTATTGATGGTATCGATGAATCGTTTGAGATTGAAGGGGCAAAGCAGAAGCTACATGATGTTTGTTCCAAGAGAAAGTGGCAGAAACCAAATTACAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGATATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAAGATGCAGAGAACTCAGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCCTCTCTAA

Coding sequence (CDS)

ATGGAATCCTCCGTCATTGCCCTTGAGCGCATTCTTTCCTATAACTTTAACAACAAGAGTCTCTTAGAAGAAGCCCTCACTCACCCCTCTTACTCTGCCTCTGCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGCTTCTCCTCACCTCCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATTCACCTGCTATGGATGTTAAGGTTAAAGAGTTTGTTGATTCTGTTGACCTAGAAGGTAGCTCCGTTCCTTATGGTAGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAGTTTGCCAAAGAAATGGAAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAATAGCATCGCGAGTGTCTATGTTGATGGTAAATTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATTAAAACATTTGTAACTATTGATGGTATCGATGAATCGTTTGAGATTGAAGGGGCAAAGCAGAAGCTACATGATGTTTGTTCCAAGAGAAAGTGGCAGAAACCAAATTACAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGATATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAAGATGCAGAGAACTCAGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCCTCTCTAA
BLAST of CSPI04G11360 vs. Swiss-Prot
Match: RTL2_ARATH (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana GN=RTL2 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.3e-97
Identity = 188/333 (56.46%), Postives = 253/333 (75.98%), Query Frame = 1

Query: 4   SVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPHL 63
           S+ A+E+IL+Y F+NKSLL+EA+TH S +   SYERLEF+GDSAIGLAI+NY +L  P L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGS-SVPYG 123
               LSLLRAANVSTEKLARV++ HGLY+++RR++P++D KVKEF ++V  E   SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 RLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQ 183
            L+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL++LQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 VCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMDT 243
           +C ++ K++DIK+WK+G+ SIA +Y+D + +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFPV 298

Query: 244 RIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI-A 303
                + ID      ++  AK KL+++C K+KW KP YSVE+D      + FVCS KI  
Sbjct: 299 E----MVIDEDSVEIQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
           T   T Y+ GDE+S++K AE+S+A  MIRAL++
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRK 387

BLAST of CSPI04G11360 vs. Swiss-Prot
Match: RTL2_ORYSJ (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-67
Identity = 142/277 (51.26%), Postives = 192/277 (69.31%), Query Frame = 1

Query: 8   LERILSYNFNNKSLLEEALTHPSYSASA-SYERLEFVGDSAIGLAITNYFFLASPHLHQG 67
           +ER+L Y F +  LLEEALTH S++  A SY+RLEFVGDSA+GLA +N+ +L +P L  G
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFADDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGPG 99

Query: 68  HLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEG----SSVPY- 127
            LS LRAAN+STEKLARVAV H LY  +RR+ P +D+ V +F+++V  E     S+VPY 
Sbjct: 100 PLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPYG 159

Query: 128 GRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLF 187
           G ++KAPKVLADIVE++A AVYVD  FDL+KLW + R L EPI T E   ++ QPVT+L 
Sbjct: 160 GSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAE--TIDEQPVTMLH 219

Query: 188 QVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMD 247
           ++CQ++GK    K W+ G  ++ +V+V G+ V  GSS QK IA+LNAAR+A  KL+ +  
Sbjct: 220 ELCQKHGKMAQFKTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDATRKLAGAKK 279

Query: 248 TRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKP 279
             + T V     DE  E+   KQKL++ CS++ W KP
Sbjct: 280 QVLTTGVGNGLGDEIGELRECKQKLNEQCSRQNWPKP 314

BLAST of CSPI04G11360 vs. Swiss-Prot
Match: DCL1_ORYSJ (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica GN=DCL1 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-47
Identity = 122/338 (36.09%), Postives = 187/338 (55.33%), Query Frame = 1

Query: 8    LERILSYNFNNKSLLEEALTHPSYSAS--ASYERLEFVGDSAIGLAITNYFFLASPHLHQ 67
            L+ +L   F NK LL EA+TH S  +S  + Y+RLEFVGD+ +   IT + F     L  
Sbjct: 1541 LKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTDLPP 1600

Query: 68   GHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGRL- 127
            G L+ LRAA V+ E  ARVAV H L+ ++R  S A++ +++EFV  V  E     +    
Sbjct: 1601 GRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGFNSFG 1660

Query: 128  ---MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLF 187
                KAPKVL DIVES+AGA+++D  +D   +W + + LL P+ T E L +   PV  L 
Sbjct: 1661 LGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPM--HPVRELQ 1720

Query: 188  QVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMD 247
            + CQ+  + ++ K  + G+ +   V+VDG  +    + QK++A+  AAR AL+ L +  +
Sbjct: 1721 ERCQQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNALVVLKEK-E 1780

Query: 248  TRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKIA 307
            T  K     DG  ++      +Q L+D+C +R+W  P Y    + GP+H + FV SV++ 
Sbjct: 1781 TATKKEDERDGEKKNGAQMFTRQTLNDICLRRQWPMPQYRCVNEGGPAHAKRFVYSVRVN 1840

Query: 308  TC--YGTFYIVGDEKSRVKDAENSAASLMIRALQERKP 338
            T     T   +G+    VK A++SAA L++  L    P
Sbjct: 1841 TSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNRDFP 1875

BLAST of CSPI04G11360 vs. Swiss-Prot
Match: DCL1_ARATH (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana GN=DCL1 PE=1 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-47
Identity = 122/349 (34.96%), Postives = 190/349 (54.44%), Query Frame = 1

Query: 6    IALERILSYNFNNKSLLEEALTHPSYSAS--ASYERLEFVGDSAIGLAITNYFFLASPHL 65
            + LER L Y F  K LL EA+TH S  +S  + Y+RLEFVGD+ +   IT + F     L
Sbjct: 1560 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1619

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGR 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  S A++ +++EFV  V  E S   +  
Sbjct: 1620 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1679

Query: 126  L----MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
                  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1680 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1739

Query: 186  LFQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1740 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1799

Query: 246  DSMDTRIKTFVTIDGIDESFEIEG----------AKQKLHDVCSKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D+C ++ W  P+Y   K+ G
Sbjct: 1800 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1859

Query: 306  PSHERIFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H + F   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1860 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

BLAST of CSPI04G11360 vs. Swiss-Prot
Match: RTL3_ARATH (Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana GN=RTL3 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.6e-44
Identity = 108/257 (42.02%), Postives = 168/257 (65.37%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           M+SSV A+E+IL+Y+F NK+LL+E LTH +   S  ++ L FVG+SA+ LA T + +L  
Sbjct: 411 MDSSVEAVEKILNYSFVNKTLLKELLTHNN---SPLFQGLMFVGESALSLAFTKHLYLTY 470

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P L    LS+LR AN   +K A VAV  G+Y       P  +    +F++ +  E    P
Sbjct: 471 PMLEPKDLSVLRDANTCHDKYACVAVKKGIYQSFIGSVPKPEKMTTDFIELMGKEDD--P 530

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVE-PQPVTV 180
           Y R++KAPK+L +++  VAGAVY+DV +++Q+L  I R LLEPIYTL++L+++  QP  +
Sbjct: 531 Y-RVVKAPKILVNLLAGVAGAVYIDVKYNVQRLLEIFRVLLEPIYTLDDLRMQLKQPFLM 590

Query: 181 LFQVCQRNGKQVDIKHWKNGSN--SIASVYVDGKFVASGSSMQKEIARLNAAREALIKLS 240
           LF++  ++GKQ+D ++ K+G +  +IA VY+D  F+ASG + + + A+L AA EA+ KLS
Sbjct: 591 LFRLADKHGKQIDFRYSKDGGSRKNIAQVYMDDMFIASGCAKRIDTAKLLAAEEAIQKLS 650

Query: 241 DSMDTRIKTFVTIDGID 255
           + M   I+  +  D +D
Sbjct: 651 ECMP--IEKIIHQDNLD 659

BLAST of CSPI04G11360 vs. TrEMBL
Match: A0A0A0L1E2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G192230 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 9.0e-183
Identity = 331/338 (97.93%), Postives = 334/338 (98.82%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLA+TNYFFLAS
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMD KVKEFVDSV LEGSSVP
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG +MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQVCQRNGKQVDIKHWKNGSNSIASV+VDGKFVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVHVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of CSPI04G11360 vs. TrEMBL
Match: A0A067F3Y1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017515mg PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-121
Identity = 219/334 (65.57%), Postives = 267/334 (79.94%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           ME +V  +E I++YNF NK LLEEALTH SY+ S SY+RLEF+GD+A+GLA++NY FLA 
Sbjct: 32  MEDAVFYVENIINYNFKNKRLLEEALTHSSYTDSTSYQRLEFIGDAALGLALSNYVFLAY 91

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P L  G LSLLRAAN+STEKLARVAV HG Y +VR  + A+D KVKEF ++V  E ++  
Sbjct: 92  PQLDPGQLSLLRAANISTEKLARVAVKHGFYKFVRHSAAALDDKVKEFAEAVSQEDNTAV 151

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG  +KAPKVLADIVES+A A+YVD++FDLQKLW+I RGLLEPI TLEELQ +PQPVT+L
Sbjct: 152 YGGSIKAPKVLADIVESIAAAIYVDIDFDLQKLWMIFRGLLEPIVTLEELQQQPQPVTLL 211

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F++CQ+NGKQVDIKHW+    +IASVYVDG FVAS SS QKEIA+LNAAREAL KL+ SM
Sbjct: 212 FKLCQKNGKQVDIKHWRKNRKNIASVYVDGSFVASASSEQKEIAKLNAAREALEKLAQSM 271

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
                     D IDE FEIE AKQKLH++C K+KW KP+Y +EKD GPSH++ F+ +V+I
Sbjct: 272 PVNCDVCEIFDDIDEKFEIEAAKQKLHELCGKKKWPKPSYKIEKDEGPSHDKKFMSAVQI 331

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
           AT  G  +I GDEKSRVKDAENSAAS+M+RAL+E
Sbjct: 332 ATTDGVLFITGDEKSRVKDAENSAASMMLRALRE 365

BLAST of CSPI04G11360 vs. TrEMBL
Match: V4TYN2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008694mg PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 5.7e-121
Identity = 218/334 (65.27%), Postives = 267/334 (79.94%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           ME +V  +E I++YNF NK LLEEALTH SY+ S SY+RLEF+GD+A+GLA++NY FLA 
Sbjct: 32  MEDAVFYVENIINYNFKNKRLLEEALTHSSYTDSTSYQRLEFIGDAALGLALSNYVFLAY 91

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P L  G LSLLRAAN+STEKLARVAV HG Y +VR  + A+D KV+EF ++V  E ++  
Sbjct: 92  PQLDPGQLSLLRAANISTEKLARVAVKHGFYKFVRHSAAALDDKVREFAEAVSQEDNTAV 151

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG  +KAPKVLADIVES+A A+YVD++FDLQKLW+I RGLLEPI TLEELQ +PQPVT+L
Sbjct: 152 YGGSIKAPKVLADIVESIAAAIYVDIDFDLQKLWMIFRGLLEPIVTLEELQQQPQPVTLL 211

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F++CQ+NGKQVDIKHW+    +IASVYVDG FVAS SS QKEIA+LNAAREAL KL+ SM
Sbjct: 212 FKLCQKNGKQVDIKHWRKNRKNIASVYVDGSFVASASSEQKEIAKLNAAREALEKLAQSM 271

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
                     D IDE FEIE AKQKLH++C K+KW KP+Y +EKD GPSH++ F+ +V+I
Sbjct: 272 PVNCDVCEIFDDIDEKFEIEAAKQKLHELCGKKKWPKPSYKIEKDEGPSHDKKFMSAVQI 331

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
           AT  G  +I GDEKSRVKDAENSAAS+M+RAL+E
Sbjct: 332 ATTDGVLFITGDEKSRVKDAENSAASMMLRALRE 365

BLAST of CSPI04G11360 vs. TrEMBL
Match: B9I149_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s08700g PE=4 SV=2)

HSP 1 Score: 440.3 bits (1131), Expect = 2.2e-120
Identity = 221/337 (65.58%), Postives = 273/337 (81.01%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           ME+SV A+E+IL+Y F NK  LEEALTH SY+ SASY+R+EF+GD+A+GLA++NY +LA 
Sbjct: 1   MEASVFAVEKILNYKFKNKRHLEEALTHSSYTDSASYQRMEFLGDAALGLALSNYVYLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGS--S 120
           P L  GHLSLLRAAN+STEKLARVA+ HGLY +VR ++ A+D KVKEF D+V  E    +
Sbjct: 61  PQLDPGHLSLLRAANISTEKLARVAIKHGLYRFVRHNATALDDKVKEFSDAVSGEDDDDA 120

Query: 121 VPYGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVT 180
           + YG  +KAPK+LADIVESVA AVYVDV+FDLQ  WVI RG+LEPI TLE+LQ +PQPVT
Sbjct: 121 LVYGGSIKAPKILADIVESVAAAVYVDVDFDLQTFWVIFRGILEPIVTLEDLQQQPQPVT 180

Query: 181 VLFQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSD 240
           +LF+ CQ+ GK VDIKHWKN + SIASVYVDG+F+AS SS QKEIA+LNAA+ AL+KLS+
Sbjct: 181 MLFERCQKQGKNVDIKHWKNEAKSIASVYVDGQFIASSSSEQKEIAKLNAAKGALLKLSN 240

Query: 241 SMDTRIKTFVTIDGID-ESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCS 300
           S  T +     +D  D  SFEIEGAKQKLH++C K+KW KPNY++E D+GP HE+ FVCS
Sbjct: 241 SFPTNLNNVSLLDIFDGSSFEIEGAKQKLHELCGKKKWPKPNYNIENDMGPPHEKKFVCS 300

Query: 301 VKIATCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
           V+IAT  G  Y+ GDE++RVKDAENSAAS MIRALQ+
Sbjct: 301 VQIATVDGILYVTGDERTRVKDAENSAASSMIRALQD 337

BLAST of CSPI04G11360 vs. TrEMBL
Match: A0A061F5X1_THECC (Ribonuclease 3-like protein 2 OS=Theobroma cacao GN=TCM_030826 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 6.3e-120
Identity = 228/334 (68.26%), Postives = 265/334 (79.34%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           ME+S+ A+ERIL+Y+F NK LLEEALTH SYS S SY+RLEFVGD+A+GLA+TN+ FLA 
Sbjct: 43  MEASIAAVERILNYSFRNKRLLEEALTHSSYSDSTSYQRLEFVGDAALGLALTNHVFLAY 102

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P L  G LSL+RAAN+STEKLARVA+ H L+ +VR ++ +++ KV+EF   V  E   V 
Sbjct: 103 PELDPGLLSLIRAANISTEKLARVAIKHRLFQFVRHNATSLEDKVREFAKVVSQEDDPVS 162

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG  MKAPKVLADIVESVA AVYVDVNFDL KLWVIIRGLLEPI TLE+LQ +PQPVTVL
Sbjct: 163 YGGSMKAPKVLADIVESVAAAVYVDVNFDLSKLWVIIRGLLEPIVTLEDLQQQPQPVTVL 222

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+ CQ+ GK VDIKHWK G  ++ASVYVDG FVASGSS QK+IA+LNAAR AL KL  SM
Sbjct: 223 FEFCQKQGKHVDIKHWKKGLRNVASVYVDGSFVASGSSEQKDIAKLNAARGALHKLLQSM 282

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
              +  F  I GI+ SFEI+GAKQKLH++C K+KW KP Y +EKD G  HERIFV SVKI
Sbjct: 283 PVDVNPF-GIGGINGSFEIDGAKQKLHELCGKKKWPKPIYELEKDEGRPHERIFVSSVKI 342

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            T  G  YI GDEKSRVK+AENSAAS MIRALQE
Sbjct: 343 PTVDGALYITGDEKSRVKEAENSAASFMIRALQE 375

BLAST of CSPI04G11360 vs. TAIR10
Match: AT3G20420.1 (AT3G20420.1 RNAse THREE-like protein 2)

HSP 1 Score: 355.1 bits (910), Expect = 4.7e-98
Identity = 188/333 (56.46%), Postives = 253/333 (75.98%), Query Frame = 1

Query: 4   SVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPHL 63
           S+ A+E+IL+Y F+NKSLL+EA+TH S +   SYERLEF+GDSAIGLAI+NY +L  P L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGS-SVPYG 123
               LSLLRAANVSTEKLARV++ HGLY+++RR++P++D KVKEF ++V  E   SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 RLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQ 183
            L+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL++LQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 VCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMDT 243
           +C ++ K++DIK+WK+G+ SIA +Y+D + +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFPV 298

Query: 244 RIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI-A 303
                + ID      ++  AK KL+++C K+KW KP YSVE+D      + FVCS KI  
Sbjct: 299 E----MVIDEDSVEIQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
           T   T Y+ GDE+S++K AE+S+A  MIRAL++
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRK 387

BLAST of CSPI04G11360 vs. TAIR10
Match: AT1G01040.2 (AT1G01040.2 dicer-like 1)

HSP 1 Score: 191.4 bits (485), Expect = 8.9e-49
Identity = 122/349 (34.96%), Postives = 190/349 (54.44%), Query Frame = 1

Query: 6    IALERILSYNFNNKSLLEEALTHPSYSAS--ASYERLEFVGDSAIGLAITNYFFLASPHL 65
            + LER L Y F  K LL EA+TH S  +S  + Y+RLEFVGD+ +   IT + F     L
Sbjct: 1561 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1620

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGR 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  S A++ +++EFV  V  E S   +  
Sbjct: 1621 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1680

Query: 126  L----MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
                  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1681 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1740

Query: 186  LFQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1741 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1800

Query: 246  DSMDTRIKTFVTIDGIDESFEIEG----------AKQKLHDVCSKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D+C ++ W  P+Y   K+ G
Sbjct: 1801 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1860

Query: 306  PSHERIFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H + F   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1861 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1907

BLAST of CSPI04G11360 vs. TAIR10
Match: AT5G45150.1 (AT5G45150.1 RNAse THREE-like protein 3)

HSP 1 Score: 180.3 bits (456), Expect = 2.1e-45
Identity = 108/257 (42.02%), Postives = 168/257 (65.37%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           M+SSV A+E+IL+Y+F NK+LL+E LTH +   S  ++ L FVG+SA+ LA T + +L  
Sbjct: 411 MDSSVEAVEKILNYSFVNKTLLKELLTHNN---SPLFQGLMFVGESALSLAFTKHLYLTY 470

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P L    LS+LR AN   +K A VAV  G+Y       P  +    +F++ +  E    P
Sbjct: 471 PMLEPKDLSVLRDANTCHDKYACVAVKKGIYQSFIGSVPKPEKMTTDFIELMGKEDD--P 530

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVE-PQPVTV 180
           Y R++KAPK+L +++  VAGAVY+DV +++Q+L  I R LLEPIYTL++L+++  QP  +
Sbjct: 531 Y-RVVKAPKILVNLLAGVAGAVYIDVKYNVQRLLEIFRVLLEPIYTLDDLRMQLKQPFLM 590

Query: 181 LFQVCQRNGKQVDIKHWKNGSN--SIASVYVDGKFVASGSSMQKEIARLNAAREALIKLS 240
           LF++  ++GKQ+D ++ K+G +  +IA VY+D  F+ASG + + + A+L AA EA+ KLS
Sbjct: 591 LFRLADKHGKQIDFRYSKDGGSRKNIAQVYMDDMFIASGCAKRIDTAKLLAAEEAIQKLS 650

Query: 241 DSMDTRIKTFVTIDGID 255
           + M   I+  +  D +D
Sbjct: 651 ECMP--IEKIIHQDNLD 659

BLAST of CSPI04G11360 vs. TAIR10
Match: AT4G15417.1 (AT4G15417.1 RNAse II-like 1)

HSP 1 Score: 150.2 bits (378), Expect = 2.3e-36
Identity = 97/231 (41.99%), Postives = 139/231 (60.17%), Query Frame = 1

Query: 7   ALERILSYNFNNKSLLEEALTHPSY--SASASYERLEFVGDSAIGLAITNYFFLASPHLH 66
           +LE+IL+Y F +KSLL +A T  SY    S SYE LE +GDS + + I   F    P   
Sbjct: 31  SLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDSILNMGIIYDFIKLYPKEA 90

Query: 67  QGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGRL 126
            G L+ LRA NV TEKLARVAV H LY+Y+R   P ++ ++ EFV++  +E   +    L
Sbjct: 91  PGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVEA--MEKYPLHSNGL 150

Query: 127 MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVC 186
           +K PKVLADIVES  GA+++D N   + +W +I+ LLEPI  L+++  +  P+T L ++C
Sbjct: 151 LKVPKVLADIVESTIGAIFMDCN-STETVWKVIKPLLEPIIHLDKM--KSHPMTELNEMC 210

Query: 187 Q-RNGKQVDIKHWKNGSNSIASVYVDGKFVASG-SSMQKEIARLNAAREAL 234
           Q RN K      W+   N     +++ KFV  G   ++KE AR  AA+ A+
Sbjct: 211 QKRNLKLTSKDTWE--ENQTYCFHIEDKFVGCGHHPVKKETARNFAAKNAI 254

BLAST of CSPI04G11360 vs. TAIR10
Match: AT3G43920.2 (AT3G43920.2 dicer-like 3)

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-29
Identity = 80/241 (33.20%), Postives = 132/241 (54.77%), Query Frame = 1

Query: 2    ESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASP 61
            E  +I LER + + F+ K LL+EA+TH S   S SYERLEF+GDS +   IT + F    
Sbjct: 1195 EDELIELERKIQHEFSAKFLLKEAITHSSLRESYSYERLEFLGDSVLDFLITRHLFNTYE 1254

Query: 62   HLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSV---DLEGSS 121
                G ++ LR+A V+ E  A+VAV + L+ +++R +  ++ ++ +++ S    D  G S
Sbjct: 1255 QTGPGEMTDLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRS 1314

Query: 122  VPYGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVT 181
            +P    ++ PK L D+VES+AGA+ +D   DL ++W +   LL P+ T ++LQ+   P  
Sbjct: 1315 IP---SIQGPKALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQL--PPYR 1374

Query: 182  VLFQVCQRNGKQVDIKHWKNGSNSIASVYV---DGKFVASGSSMQKEIARLNAAREALIK 237
             L ++C   G    +K   +G  + A++ +   D      GS    ++A   AA   L +
Sbjct: 1375 ELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTNKLALGKAASHLLTQ 1430

BLAST of CSPI04G11360 vs. NCBI nr
Match: gi|778697607|ref|XP_011654357.1| (PREDICTED: ribonuclease 3-like protein 2 [Cucumis sativus])

HSP 1 Score: 647.5 bits (1669), Expect = 1.3e-182
Identity = 331/338 (97.93%), Postives = 334/338 (98.82%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLA+TNYFFLAS
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMD KVKEFVDSV LEGSSVP
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG +MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQVCQRNGKQVDIKHWKNGSNSIASV+VDGKFVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVHVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of CSPI04G11360 vs. NCBI nr
Match: gi|700198793|gb|KGN53951.1| (hypothetical protein Csa_4G192230 [Cucumis sativus])

HSP 1 Score: 647.5 bits (1669), Expect = 1.3e-182
Identity = 331/338 (97.93%), Postives = 334/338 (98.82%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLA+TNYFFLAS
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMD KVKEFVDSV LEGSSVP
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG +MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQVCQRNGKQVDIKHWKNGSNSIASV+VDGKFVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVHVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of CSPI04G11360 vs. NCBI nr
Match: gi|659096529|ref|XP_008449146.1| (PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo])

HSP 1 Score: 578.9 bits (1491), Expect = 5.6e-162
Identity = 298/338 (88.17%), Postives = 311/338 (92.01%), Query Frame = 1

Query: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60
           M SSVIA+ERILSY F NK LLEEALTH SY  SASYERLEF+GDSAIGLA+TNYFFLA 
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVP 120
           P+LHQG LSLLRAANVSTEKLARVAVTHGLYNYVRR++  MD KVKEFVDSV LEGSSVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F VCQRNGKQVDIK+WKNGS +IASVYVDG FVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300
           DT+IKT VTI+GIDESFEIEGAKQKLHD C ++KWQKP YS+EK LGPSH R FVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
            TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERK L
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of CSPI04G11360 vs. NCBI nr
Match: gi|659096531|ref|XP_008449147.1| (PREDICTED: ribonuclease 3-like protein 2 isoform X1 [Cucumis melo])

HSP 1 Score: 459.5 bits (1181), Expect = 5.0e-126
Identity = 240/332 (72.29%), Postives = 275/332 (82.83%), Query Frame = 1

Query: 7   ALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPHLHQG 66
           A+ER LSY F NK LLE ALTH SY  +ASY+RLEF+GDSAIGLAI+NYFF   P L QG
Sbjct: 3   AIERKLSYKFKNKKLLEVALTHSSYPNTASYDRLEFIGDSAIGLAISNYFFSLYPDLDQG 62

Query: 67  HLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGRLMK 126
            L+LLRAANVSTEKLARVAV HGLY+YVRR +  +D  V+EF D+V LE +S+PYG L K
Sbjct: 63  RLTLLRAANVSTEKLARVAVLHGLYDYVRRSAADLDDNVREFADAVALEDNSIPYGGLKK 122

Query: 127 APKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVCQR 186
           APKVLADIVESVAGA+YVDV FDLQKLW II+ LL+PIYT E+L+ EPQPVT+LF+VCQ+
Sbjct: 123 APKVLADIVESVAGAIYVDVEFDLQKLWAIIKDLLKPIYTPEDLEAEPQPVTLLFEVCQK 182

Query: 187 NGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMDTRIKT 246
           NGKQV IK+ + GS ++A VYVD   VASGSS QK IARLNAAR+ALI LSDSMDT+IKT
Sbjct: 183 NGKQVVIKNQEKGSWNVARVYVDRTLVASGSSKQKPIARLNAARQALIILSDSMDTKIKT 242

Query: 247 FVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKIATCYGT 306
            VTIDGID SFEIEGA QKLHD CS++KWQ P+YSVE+ LG  HER FVCSVKI+T   T
Sbjct: 243 VVTIDGIDGSFEIEGATQKLHDFCSRKKWQNPSYSVEEVLGRPHERRFVCSVKISTYDDT 302

Query: 307 FYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
             I+GDEKSRVK+A+NSAASLMIR LQERK L
Sbjct: 303 HSIIGDEKSRVKEAKNSAASLMIRNLQERKYL 334

BLAST of CSPI04G11360 vs. NCBI nr
Match: gi|659096533|ref|XP_008449148.1| (PREDICTED: ribonuclease 3-like protein 2 isoform X2 [Cucumis melo])

HSP 1 Score: 453.4 bits (1165), Expect = 3.6e-124
Identity = 239/332 (71.99%), Postives = 274/332 (82.53%), Query Frame = 1

Query: 7   ALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLASPHLHQG 66
           A+ER LSY F NK LLE ALTH SY  +ASY+RLEF+GDSAIGLAI+NYFF   P L QG
Sbjct: 3   AIERKLSYKFKNKKLLEVALTHSSYPNTASYDRLEFIGDSAIGLAISNYFFSLYPDLDQG 62

Query: 67  HLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVDLEGSSVPYGRLMK 126
            L+LLRAANVSTEKLARVAV HGLY+YVRR +  +D  V+EF D+V LE +S+PYG L K
Sbjct: 63  RLTLLRAANVSTEKLARVAVLHGLYDYVRRSAADLDDNVREFADAVALEDNSIPYGGLKK 122

Query: 127 APKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVCQR 186
           APKVLADIVESVAGA+YVDV FDLQKLW II+ LL+PIYT E+L+ EPQPVT+LF+VCQ+
Sbjct: 123 APKVLADIVESVAGAIYVDVEFDLQKLWAIIKDLLKPIYTPEDLEAEPQPVTLLFEVCQK 182

Query: 187 NGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSMDTRIKT 246
           NGKQV IK+ + GS ++A VYVD   VASGSS QK IARLNAAR+ALI LSDSMDT+IKT
Sbjct: 183 NGKQVVIKNQEKGSWNVARVYVDRTLVASGSSKQKPIARLNAARQALIILSDSMDTKIKT 242

Query: 247 FVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKIATCYGT 306
            VTIDGID SFEIEGA QKLHD CS++KWQ P+YS E+ LG  HER FVCSVKI+T   T
Sbjct: 243 VVTIDGIDGSFEIEGATQKLHDFCSRKKWQNPSYS-EEVLGRPHERRFVCSVKISTYDDT 302

Query: 307 FYIVGDEKSRVKDAENSAASLMIRALQERKPL 339
             I+GDEKSRVK+A+NSAASLMIR LQERK L
Sbjct: 303 HSIIGDEKSRVKEAKNSAASLMIRNLQERKYL 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RTL2_ARATH8.3e-9756.46Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana GN=RTL2 PE=1 SV=1[more]
RTL2_ORYSJ1.8e-6751.26Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica GN=Os05g0271300 PE... [more]
DCL1_ORYSJ1.6e-4736.09Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica GN=DCL1 PE=3 SV... [more]
DCL1_ARATH1.6e-4734.96Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana GN=DCL1 PE=1 SV=2[more]
RTL3_ARATH3.6e-4442.02Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana GN=RTL3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1E2_CUCSA9.0e-18397.93Uncharacterized protein OS=Cucumis sativus GN=Csa_4G192230 PE=4 SV=1[more]
A0A067F3Y1_CITSI2.6e-12165.57Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017515mg PE=4 SV=1[more]
V4TYN2_9ROSI5.7e-12165.27Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008694mg PE=4 SV=1[more]
B9I149_POPTR2.2e-12065.58Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s08700g PE=4 SV=2[more]
A0A061F5X1_THECC6.3e-12068.26Ribonuclease 3-like protein 2 OS=Theobroma cacao GN=TCM_030826 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G20420.14.7e-9856.46 RNAse THREE-like protein 2[more]
AT1G01040.28.9e-4934.96 dicer-like 1[more]
AT5G45150.12.1e-4542.02 RNAse THREE-like protein 3[more]
AT4G15417.12.3e-3641.99 RNAse II-like 1[more]
AT3G43920.22.1e-2933.20 dicer-like 3[more]
Match NameE-valueIdentityDescription
gi|778697607|ref|XP_011654357.1|1.3e-18297.93PREDICTED: ribonuclease 3-like protein 2 [Cucumis sativus][more]
gi|700198793|gb|KGN53951.1|1.3e-18297.93hypothetical protein Csa_4G192230 [Cucumis sativus][more]
gi|659096529|ref|XP_008449146.1|5.6e-16288.17PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo][more]
gi|659096531|ref|XP_008449147.1|5.0e-12672.29PREDICTED: ribonuclease 3-like protein 2 isoform X1 [Cucumis melo][more]
gi|659096533|ref|XP_008449148.1|3.6e-12471.99PREDICTED: ribonuclease 3-like protein 2 isoform X2 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000999RNase_III_dom
IPR014720dsRBD_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004525ribonuclease III activity
Vocabulary: Biological Process
TermDefinition
GO:0006396RNA processing
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051252 regulation of RNA metabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0006396 RNA processing
biological_process GO:0030422 production of siRNA involved in RNA interference
cellular_component GO:0005575 cellular_component
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0016442 RISC complex
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G11360.1CSPI04G11360.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000999Ribonuclease III domainGENE3DG3DSA:1.10.1520.10coord: 5..166
score: 1.7
IPR000999Ribonuclease III domainPFAMPF00636Ribonuclease_3coord: 38..145
score: 4.9
IPR000999Ribonuclease III domainSMARTSM00535riboneu5coord: 20..170
score: 1.3
IPR000999Ribonuclease III domainPROSITEPS00517RNASE_3_1coord: 38..46
scor
IPR000999Ribonuclease III domainPROFILEPS50142RNASE_3_2coord: 5..147
score: 33
IPR000999Ribonuclease III domainunknownSSF69065RNase III domain-likecoord: 127..166
score: 4.19E-39coord: 3..95
score: 4.19
IPR014720Double-stranded RNA-binding domainGENE3DG3DSA:3.30.160.20coord: 262..333
score: 3.5E-14coord: 176..236
score: 2.
IPR014720Double-stranded RNA-binding domainPFAMPF00035dsrmcoord: 176..236
score: 9.3E-6coord: 262..332
score: 4.1
IPR014720Double-stranded RNA-binding domainSMARTSM00358DRBM_3coord: 262..333
score: 8.6E-11coord: 176..237
score: 0
IPR014720Double-stranded RNA-binding domainPROFILEPS50137DS_RBDcoord: 261..334
score: 10.63coord: 175..238
score: 9
NoneNo IPR availablePANTHERPTHR14950HELICASE-RELATEDcoord: 3..257
score: 1.7E
NoneNo IPR availablePANTHERPTHR14950:SF22SUBFAMILY NOT NAMEDcoord: 3..257
score: 1.7E
NoneNo IPR availableunknownSSF54768dsRNA-binding domain-likecoord: 129..236
score: 9.43E-9coord: 261..336
score: 2.83

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI04G11360Csa4G192230Cucumber (Chinese Long) v2cpicuB183
CSPI04G11360CsaV3_4G013380Cucumber (Chinese Long) v3cpicucB213
CSPI04G11360Cucsa.023960Cucumber (Gy14) v1cgycpiB513
CSPI04G11360CsGy4G010900Cucumber (Gy14) v2cgybcpiB168
The following gene(s) are paralogous to this gene:

None