CSPI03G23470 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G23470
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	ralf-like 9
Location	Chr3 : 20393978 .. 20394636 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTGAAACTGTTTCACATTTCAAGTTTGTATAAGTTTTTGTCATAAAGGATGATCATGTCAAAGAGAATGACACTTGTTTTTATTGGGATTCTCTTGGTTAGCTTCGACATGATTATGATCACAAATGCAAGAAAATTCATTGATTACGGTTCTATTGTTGCAGGAGATGTTAGCCCTGGATGTAGCCCAACGCACCCTGAACTTTGTAGAGTAAAGAGCGCAAATCCATACCAAAGAGGTTGCAATCGAATCGATCGTTGTAGAGAGGGAAATGATATTATTGACGCTGAAGAAGAACATATTGAAGGGGATGCATCGATTAGCCCTTCAATAAGCCCTAATACAGAATTAAATCTTGATTGAATTGAATTGATATAATATTTATTTATATTTATCTAAAACAAAATTATTTATGTCTAGTCATTTGATGAATGATTGAATAATGTAATCATTAGATGTCTGACAAGGCTTGAAAGCTATGTGTAATATGTAACTGGTAAGGTTGCTTAAAGGACAATTATGCAACAATATATGCTAATTTTTATGGAGAATTGAAAAATAGCTGCAAATGTAAGTAATTTGGAAAAGGTAAAGACGGACCAATTCACCTTGACAAAAAAATTATTTGTAAAAACCCTTGCTTATTACTGTACTGTG

mRNA sequence

ATGATCATGTCAAAGAGAATGACACTTGTTTTTATTGGGATTCTCTTGGTTAGCTTCGACATGATTATGATCACAAATGCAAGAAAATTCATTGATTACGGTTCTATTGTTGCAGGAGATGTTAGCCCTGGATGTAGCCCAACGCACCCTGAACTTTGTAGAGTAAAGAGCGCAAATCCATACCAAAGAGGTTGCAATCGAATCGATCGTTGTAGAGAGGGAAATGATATTATTGACGCTGAAGAAGAACATATTGAAGGGGATGCATCGATTAGCCCTTCAATAAGCCCTAATACAGAATTAAATCTTGATTGA

Coding sequence (CDS)

ATGATCATGTCAAAGAGAATGACACTTGTTTTTATTGGGATTCTCTTGGTTAGCTTCGACATGATTATGATCACAAATGCAAGAAAATTCATTGATTACGGTTCTATTGTTGCAGGAGATGTTAGCCCTGGATGTAGCCCAACGCACCCTGAACTTTGTAGAGTAAAGAGCGCAAATCCATACCAAAGAGGTTGCAATCGAATCGATCGTTGTAGAGAGGGAAATGATATTATTGACGCTGAAGAAGAACATATTGAAGGGGATGCATCGATTAGCCCTTCAATAAGCCCTAATACAGAATTAAATCTTGATTGA

Homology

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BLAST of CSPI03G23470 vs. Swiss-Prot
Match: RLF9_ARATH (Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-07
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--ARKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + LV F  +  T   A ++I Y +I  GD +  C   HP  C+ K A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYQRGCNRIDRCREG 75
          NPYQRGC +I+RCR G
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of CSPI03G23470 vs. TrEMBL
Match: A0A0A0L8B1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 2.6e-48
Identity = 99/100 (99.00%), Postives = 99/100 (99.00%), Query Frame = 1


Query: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60
           MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP
Sbjct: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNTE 101
           YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPN E
Sbjct: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIE 100

[Close]

BLAST of CSPI03G23470 vs. TrEMBL
Match: A0A067F0E4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041712mg PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.7e-07
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN---ARKFIDYGSIVAGDVSPGCSPTHPELCRVKS 60
          M+MSKRM +VF  I++++  ++M+       K I YG+I AG  +PGCSP HPELC+++ 
Sbjct: 1  MMMSKRM-MVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEP 60

Query: 61 ANPYQRGCNRIDRCR 73
           NPY RGC    +CR
Sbjct: 61 GNPYHRGCENEAQCR 73

[Close]

BLAST of CSPI03G23470 vs. TrEMBL
Match: V4UF46_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018043mg PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.7e-07
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN---ARKFIDYGSIVAGDVSPGCSPTHPELCRVKS 60
          M+MSKRM +VF  I++++  ++M+       K I YG+I AG  +PGCSP HPELC+++ 
Sbjct: 1  MMMSKRM-MVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEP 60

Query: 61 ANPYQRGCNRIDRCR 73
           NPY RGC    +CR
Sbjct: 61 GNPYHRGCENEAQCR 73

[Close]

BLAST of CSPI03G23470 vs. TAIR10
Match: AT1G61566.1 (AT1G61566.1 ralf-like 9)

HSP 1 Score: 57.4 bits (137), Expect = 6.2e-09
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--ARKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + LV F  +  T   A ++I Y +I  GD +  C   HP  C+ K A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYQRGCNRIDRCREG 75
          NPYQRGC +I+RCR G
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of CSPI03G23470 vs. TAIR10
Match: AT2G22055.1 (AT2G22055.1 RALF-like 15)

HSP 1 Score: 49.3 bits (116), Expect = 1.7e-06
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--ARKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + L+ F  +  T   A ++I Y  +  GD +  C   HP  C+ + A
Sbjct: 1  MGMSKSIKVI-VSLALILFLALAATKVEATRYISYRGMNHGDHAIHCDKAHPNTCKKQVA 60

Query: 61 NPYQRGCNRIDRCR 73
          NPY+RGC  I+RCR
Sbjct: 61 NPYRRGCGTIERCR 73

[Close]

BLAST of CSPI03G23470 vs. NCBI nr
Match: gi|700202865|gb|KGN57998.1| (hypothetical protein Csa_3G426350 [Cucumis sativus])

HSP 1 Score: 199.1 bits (505), Expect = 3.8e-48
Identity = 99/100 (99.00%), Postives = 99/100 (99.00%), Query Frame = 1


Query: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60
           MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP
Sbjct: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNTE 101
           YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPN E
Sbjct: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIE 100

[Close]

BLAST of CSPI03G23470 vs. NCBI nr
Match: gi|567910725|ref|XP_006447676.1| (hypothetical protein CICLE_v10018043mg [Citrus clementina])

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-07
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN---ARKFIDYGSIVAGDVSPGCSPTHPELCRVKS 60
          M+MSKRM +VF  I++++  ++M+       K I YG+I AG  +PGCSP HPELC+++ 
Sbjct: 1  MMMSKRM-MVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEP 60

Query: 61 ANPYQRGCNRIDRCR 73
           NPY RGC    +CR
Sbjct: 61 GNPYHRGCENEAQCR 73

The following BLAST results are available for this feature:

BLAST of CSPI03G23470 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF9_ARATH	1.1e-07	42.11	Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1	[more]

BLAST of CSPI03G23470 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8B1_CUCSA	2.6e-48	99.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1	[more]
A0A067F0E4_CITSI	1.7e-07	46.67	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041712mg PE=4 SV=1	[more]
V4UF46_9ROSI	1.7e-07	46.67	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018043mg PE=4 SV=1	[more]

BLAST of CSPI03G23470 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G61566.1	6.2e-09	42.11	ralf-like 9	[more]
AT2G22055.1	1.7e-06	36.49	RALF-like 15	[more]

BLAST of CSPI03G23470 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202865\|gb\|KGN57998.1\|	3.8e-48	99.00	hypothetical protein Csa_3G426350 [Cucumis sativus]	[more]
gi\|567910725\|ref\|XP_006447676.1\|	2.5e-07	46.67	hypothetical protein CICLE_v10018043mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G23470.1	CSPI03G23470.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 26..72 score: 2.
None	No IPR available	PANTHER	PTHR34270	FAMILY NOT NAMED	coord: 1..84 score: 3.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G23470	Csa3G426350	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G23470	CsaV3_3G023270	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G23470	Cucsa.017030	Cucumber (Gy14) v1	cgycpiB369

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G23470	Melon (DHL92) v3.5.1	cpimeB201
CSPI03G23470	Watermelon (Charleston Gray)	cpiwcgB242
CSPI03G23470	Watermelon (97103) v1	cpiwmB247
CSPI03G23470	Cucurbita pepo (Zucchini)	cpecpiB549
CSPI03G23470	Cucurbita pepo (Zucchini)	cpecpiB779
CSPI03G23470	Bottle gourd (USVL1VR-Ls)	cpilsiB194
CSPI03G23470	Melon (DHL92) v3.6.1	cpimedB195
CSPI03G23470	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G23470	Silver-seed gourd	carcpiB0939
CSPI03G23470	Silver-seed gourd	carcpiB1053
CSPI03G23470	Watermelon (97103) v2	cpiwmbB225
CSPI03G23470	Cucurbita maxima (Rimu)	cmacpiB015
CSPI03G23470	Cucurbita maxima (Rimu)	cmacpiB460
CSPI03G23470	Cucurbita moschata (Rifu)	cmocpiB006
CSPI03G23470	Cucurbita moschata (Rifu)	cmocpiB448