CSPI01G29460 (gene) Wild cucumber (PI 183967)

NameCSPI01G29460
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionE3 ubiquitin-protein ligase RING1-like protein
LocationChr1 : 23927942 .. 23931835 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCGGAAGAACTGAATTTTACAGAATTATAAGTTTTTAACAATTTGGGTGAATCAGTCAATCGGAAAACTGCCTCTGGGACCCCACGGAGCAAAATTTTGTTGCTCAGGGTCCTCTCGTTCTCTCTCTCTCTCCGCTCTTGATCCAAATCTTCATTCACTGTTCTTGTTTCTCCTTCTTCCCCAACCCTCCCACTTCCCCTACTTCAGCTTCGGTACGTCTTTCTTCACTTCAATTTTAAATCAGGGTTCCATTTTTCAATTTCTGCGTCTTTCTAATTTTGTTTTGATACCGTATTCATATACTTCTTGTTGATTTTATATCTGTCGTAATTCTGCTTAATTTCCATGTTTTTTCCTAGTCTTTTCTTGTAATTCTTTCGATATATCTCAATCTGACGAGGGTTTGAACGCCTCGTTTGAGTGATGATTCTGTAACGAGGGAAATCTTCCTCTCGGTTATAGGAGACAACTCAAAAATAAATTCGGATTAGGTTGGGTCTCAATTCAATATATGTATTTTTGGGTTGATCCAATCCGACTCGAATTTCATATGTATTAATAAAACATTATGTATGCATTTCTTTTAGAATGGATGAATTTTTAATGTCTTTTTTAGAAATGTTTTCTTTTGTTTAAGTTTACAATAGATATCATGGATATTTGAGCATTTAAGTTTTATAAGATTTTAGTTTAAATTCATGTATTTTAGATTAATAAGAAAAAAAAAAAAAAAGACCCAACAACCCAACCTGAATTTTGAGGGTTGGATTGGAGAGAAGACCCTGTTGGAACTGGGAGTCTTTTGAATTGCCAACTCAACCAACATGAATTTTCGGGTTGGTCCAAAAATTTCCCACAACTCAACCTAAGTACTACCTAAACTGTCTCCTTCGCTTGTGAGAGATAAAGTATGTGGGGATTGTACAGATGATACATCAAGAAGTTTGTCAGAAGAATTGGGGACAGTGATTGTACTCGTATTAATTCCTGGCTATCAAGTTGAAATCATGGGGTTATTAGGAGGCTATTCAAGTTTAGTTGTGTTTATATAAGCCACATGAATGTGTTTGGGATTTTTCATTTTTTATTATTTATGTTACATTTTTAGTCTATATGTTTCCATTTTTGTTCATAGTTTTCTTTTATCTATCAAGGCTTACCCAAGAGTCTTACCAATAATCAAATGTCACATATCCAATCTAGTAAGATGTATTTTTCAACCATAATTGTACTTAAAATCTGACCTTGTTAAGACATAAAGTCAATAGAATATTCTTTGCAATTATGTATTTATTTACCTTGATCTGACTTTTGCCAATATGGCATGATTTGGATTATTTTCCGTATGTAATAGTTCTCAATAGTACTATTCTAGTTCATTATAAAAACCATTTTCTTCTCTTGGCAGATGCACAAGCCGAGAGAACGTTATTAAAACCAACCCCTTACTGTAAACCTTTTCTGACCCCAACGGGTGTTTTTCAGTTAATATGATCTAAGTATGCTACAAGGGAGTTGGGTTATCTTTTACTACATTGGGGGATTTTTGGTACCCTCTAATTAAACAAACATTTTTTGAAGTACACAGAATCAAAACGATATATAGGTGAAAATATAGAGAAGAGTACAATGGAATTAAATCTGCTTTTCTCTTGAATTGTGATTGTCCATTATCCAATCGAGGAGTATATTCCTAGATTCCTTTGCAGACTAATTTGATGTGGCTTGCTTGGCTGGGCTAAAGTTTATACAACATCAATTCTTTTTTAGATTACGGGTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGTGTTTAACCTTTTATCGTTGATGCTATCTTCACCCTTACTTTTGTGTGTGTGTGTGTGTGTGTTTAATATATAATGAATGAAATCATCACCAGATATTTGTCGATTTTAAGGAAATATATTGATTTAAACTTAGACCCTATTTTCTAACCATTTGATTTCTTAAATTTGCGCTTGTTTCACACCAAAACTTTAGAATGGTTTTCATCATTCCTAAACAAACATGTGAATTCTTAGGCAATTTCCAACAGCAGGAACAAGTTTCCAAAATCTACTTTTCTTAGTTTTAGAAAATGGGCTTGATTTTGGAAACAAATTATGTGACAAGGAAACTCTAATGTACAAGTAGTTCTTTATAAGCTTAATCTCCAAAAACCGAACGGTTATCTAACAGGTTAGATCATTATGTGCTTTCCAAAAGTTGTATTGGGATGAATTGTTGTGGACAAATGTTTGATTTTGTCTTCAAAATTGATTTTCTTAACCAATGGGCATTTCATTGCAGAATTCATACATTTAGGAATAAGCCTGGGTTTTTTTTTTCAACTGTAGCAATTGAGAGAAGGATGTCATCTTTTGGTGAAGACACCCATTGGTGCCATCAATGCCACCATTCATTCTGGCTTGATGGAGAAGACATAGTTTGCCCTCATTGTTATGGTGGTTTCGTCGAAGAACTAAACGATGAACATGATGAAACAGTTCAAAATGACTTTAATCCAGGCATAGAAGAAGATCTTTCAACCCAGGTGCCTCCAATTTTCGAAGCTATGTTTGCATTAATGGGACGAAGAAGTCCTTATCCAAGATTTGGTCTTCTCGAAGCTGTTGATACTTTCACCAGAGAGAGAATGGCTGGAAGAAATCCTAACTTTGACGTCAGGAGAAGATCTGGTTCTGTTCCAGGGCAGAATTTGGATTTTTTCAACTCTTTTTGGTCATTCCATGATCACATGTCGGGTTCGACCTTCGCTAATGTCACTCCAGAAGGTAGATCAAGTCAGCATACGGGACTGGAAGAATTAGCCGCACAACTTTCCTTAAATGAACAAAGAGAACCAGTCCCAACTCCTGCTTCTCACTCTTGTATAGAAGCAATGCCGACAATCAAGATTAACCAAATGCACCTCGGCACCGACTCTCATTGTCCAGTCTGTAAAGAAAAATTTGAGCTCGAATCTGAAGCCAAAGCTTTGCCATGTAATCATATCTATCACAATGATTGCATTCTTCCTTGGCTGGTCCAACATAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGAAAGTGGACACAGTTGGGGAGGGAGTGGTGACAACAACAGTGAAGACCTAAATGAGAGAGAGATTACTCAACGAAACCTGAGAAGGAGGCATCCGATCCCAGATTTCTTTGCTCCTCGTCGAATTGTCACTCGTTTCAGCAGTGGGATTCATATAAATGGCCTATCTTCAGATCGAGATCACGATAACGAGATGGGTTACCCTGAATGACGACCTTTCCATTACCATGTCTACAACTCTTCTCTACTTTAGAAGCTGCTGAGCATGATATGGGAAAGAAAGCTGTGTTCTACAACGAATGAGGTAATAAGAAATAAGAATCAGCTCTAGAGGAGTTGTAATATCTAGAGCTGTTTTTTTCTTCCACCTTTTTTTTCCCTTCCTTTTGTGTAAAATGATATGAAAAAAAATCTCATATCTCCTACAATGACTTGTACTGGGAAAAAAGGGGCCCTAGAGATGAAATCAAATGTAATTATAGTTGATATGTCCCATGGATCAATTGAAGCCATTTGCCTTTTCTGATTTGTGAGCTTCAATGATGAGAAGAAGATTTGAAAAGGAGCAATAGATTTTATTGTGTTTATATAATGTTGTGCATTTTGGCATTTGTTATAATCCAAAGACATTAAATTTCCGTCTGTCTATCCCCCCTTATTTCCACCCGT

mRNA sequence

ATGTCATCTTTTGGTGAAGACACCCATTGGTGCCATCAATGCCACCATTCATTCTGGCTTGATGGAGAAGACATAGTTTGCCCTCATTGTTATGGTGGTTTCGTCGAAGAACTAAACGATGAACATGATGAAACAGTTCAAAATGACTTTAATCCAGGCATAGAAGAAGATCTTTCAACCCAGGTGCCTCCAATTTTCGAAGCTATGTTTGCATTAATGGGACGAAGAAGTCCTTATCCAAGATTTGGTCTTCTCGAAGCTGTTGATACTTTCACCAGAGAGAGAATGGCTGGAAGAAATCCTAACTTTGACGTCAGGAGAAGATCTGGTTCTGTTCCAGGGCAGAATTTGGATTTTTTCAACTCTTTTTGGTCATTCCATGATCACATGTCGGGTTCGACCTTCGCTAATGTCACTCCAGAAGGTAGATCAAGTCAGCATACGGGACTGGAAGAATTAGCCGCACAACTTTCCTTAAATGAACAAAGAGAACCAGTCCCAACTCCTGCTTCTCACTCTTGTATAGAAGCAATGCCGACAATCAAGATTAACCAAATGCACCTCGGCACCGACTCTCATTGTCCAGTCTGTAAAGAAAAATTTGAGCTCGAATCTGAAGCCAAAGCTTTGCCATGTAATCATATCTATCACAATGATTGCATTCTTCCTTGGCTGGTCCAACATAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGAAAGTGGACACAGTTGGGGAGGGAGTGGTGACAACAACAGTGAAGACCTAAATGAGAGAGAGATTACTCAACGAAACCTGAGAAGGAGGCATCCGATCCCAGATTTCTTTGCTCCTCGTCGAATTGTCACTCGTTTCAGCAGTGGGATTCATATAAATGGCCTATCTTCAGATCGAGATCACGATAACGAGATGGGTTACCCTGAATGA

Coding sequence (CDS)

ATGTCATCTTTTGGTGAAGACACCCATTGGTGCCATCAATGCCACCATTCATTCTGGCTTGATGGAGAAGACATAGTTTGCCCTCATTGTTATGGTGGTTTCGTCGAAGAACTAAACGATGAACATGATGAAACAGTTCAAAATGACTTTAATCCAGGCATAGAAGAAGATCTTTCAACCCAGGTGCCTCCAATTTTCGAAGCTATGTTTGCATTAATGGGACGAAGAAGTCCTTATCCAAGATTTGGTCTTCTCGAAGCTGTTGATACTTTCACCAGAGAGAGAATGGCTGGAAGAAATCCTAACTTTGACGTCAGGAGAAGATCTGGTTCTGTTCCAGGGCAGAATTTGGATTTTTTCAACTCTTTTTGGTCATTCCATGATCACATGTCGGGTTCGACCTTCGCTAATGTCACTCCAGAAGGTAGATCAAGTCAGCATACGGGACTGGAAGAATTAGCCGCACAACTTTCCTTAAATGAACAAAGAGAACCAGTCCCAACTCCTGCTTCTCACTCTTGTATAGAAGCAATGCCGACAATCAAGATTAACCAAATGCACCTCGGCACCGACTCTCATTGTCCAGTCTGTAAAGAAAAATTTGAGCTCGAATCTGAAGCCAAAGCTTTGCCATGTAATCATATCTATCACAATGATTGCATTCTTCCTTGGCTGGTCCAACATAATACTTGCCCGGTTTGTCGACTCGAGCTGCCTCAGCAGGAAAGTGGACACAGTTGGGGAGGGAGTGGTGACAACAACAGTGAAGACCTAAATGAGAGAGAGATTACTCAACGAAACCTGAGAAGGAGGCATCCGATCCCAGATTTCTTTGCTCCTCGTCGAATTGTCACTCGTTTCAGCAGTGGGATTCATATAAATGGCCTATCTTCAGATCGAGATCACGATAACGAGATGGGTTACCCTGAATGA
BLAST of CSPI01G29460 vs. Swiss-Prot
Match: RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.0e-40
Identity = 119/331 (35.95%), Postives = 163/331 (49.24%), Query Frame = 1

Query: 3   SFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLSTQV 62
           S   +THWCH+C  +  L G++ VC +C GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSF---------HDHMSGSTFANVTP-----EGRSSQH---TGLEELAAQLSLNEQREP 182
              F          D+   + F   +P      G +  +    GLEEL  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPTPASHSCIEAMPTIKINQMHL-GTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPW 242
            P PA  S I+A+PTIKI Q HL  +DS+CPVCK++FEL SEAK +PCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELP--------QQESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPD 302
           LVQHN+CPVCR ELP        Q  +  +      ++S   N RE       RR+P   
Sbjct: 242 LVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFSS 301

Query: 303 FFAPRRIVTRFSS----GIHINGLSSDRDHD 304
           F+  R   +  SS    G   N  +SD +H+
Sbjct: 302 FWPFRSSGSSSSSTQNRGGTRNSDTSDENHN 308

BLAST of CSPI01G29460 vs. Swiss-Prot
Match: RDUF2_ARATH (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.0e-29
Identity = 88/273 (32.23%), Postives = 127/273 (46.52%), Query Frame = 1

Query: 8   THWCHQCHH--SFWLDGE-----DIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 67
           ++WC+ C    S W D        + CPHC GGF+E++ND      +             
Sbjct: 17  SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATE------------L 76

Query: 68  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFD--------VRRRSGSV 127
            +P   E       RRS               R R +GR P+F+           R    
Sbjct: 77  TIPASTEVRSINNNRRS-------------VIRRRRSGRRPSFNPVIVLQGGAGEREEGE 136

Query: 128 PGQNLDFFNSFWSFHDHMSGSTFANVTPEGRSS--QHTGLEELAAQLSLNEQ------RE 187
            G       +F  ++D  SGS    + P+  S     +G E L  QLS  E       R 
Sbjct: 137 EGDAARDRRAFEFYYDDGSGSGLRPL-PDSVSEILMGSGFERLLEQLSQIEASATGIGRS 196

Query: 188 PVPTPASHSCIEAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPW 247
             P PAS S IE++P ++I+  H+G++++C VC E FE E+EA+ +PC H++H+DCI+PW
Sbjct: 197 GNP-PASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPW 256

Query: 248 LVQHNTCPVCRLELPQQESGHSWGGSGDNNSED 258
           L   N+CPVCR ELP + +  S     +NN ED
Sbjct: 257 LSIRNSCPVCRFELPSEPNRRS-----NNNEED 257

BLAST of CSPI01G29460 vs. Swiss-Prot
Match: RHC2A_ARATH (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana GN=RHC2A PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.8e-28
Identity = 86/256 (33.59%), Postives = 123/256 (48.05%), Query Frame = 1

Query: 8   THWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGI--EEDLSTQVPPI 67
           ++WC+ C    W+  + I CP C GGF+E + +  D T  + F      +    T+ PP 
Sbjct: 5   SYWCYSCSRFVWVS-DSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRFPPP 64

Query: 68  FEAMF----ALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRR------SGSVPGQ 127
             +      ++    SP P         T      + R+PN  +  R      S  V  +
Sbjct: 65  SSSSSTPSASMHADNSPTP---------TIVTRTRSNRSPNPVIVLRGSAAAPSSDVVSE 124

Query: 128 NLDFFNSFWSFHDHMSGSTFANVTPEGRS----SQHTGLEELAAQLSLNEQREPVPT--- 187
            LD  ++F  ++D  + S    + P        S    L +  +Q+ LN  R        
Sbjct: 125 GLDR-SAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHP 184

Query: 188 PASHSCIEAMPTIKINQMHL--GTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLV 243
           PAS S IEA+P I+I+  HL   + SHC VCKE F L+S A+ +PCNHIYH DCILPWL 
Sbjct: 185 PASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLA 244

BLAST of CSPI01G29460 vs. Swiss-Prot
Match: R126B_XENLA (E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis GN=rnf126-b PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.2e-25
Identity = 79/277 (28.52%), Postives = 121/277 (43.68%), Query Frame = 1

Query: 9   HWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFN--------PGIEEDLST 68
           ++CH C         +  CP C  GF+EEL +    +  N  N        P  E   S 
Sbjct: 11  YFCHSCTAEITPRLPEYTCPRCDSGFIEELPETSRNSESNSSNNSGTDQNRPSFENIESA 70

Query: 69  Q--VPPIF-EAMFALMGRRSPYPRFGLLEAVDTF----------TRERMAGRNPNFDVRR 128
           Q  +P  + +  F +      +P FG    V+            +R+R   R P   +  
Sbjct: 71  QFTLPSGYGQVTFGIFNEGLDFPMFGTSGPVEETRDGESRREHQSRQRYGARQPRARMST 130

Query: 129 RSGSVPGQNLDFFNSFWS--FHDHMSGSTFANV----------TPEGRSSQHTGLEELAA 188
           R G+   + +           +  ++ +  +N+           P   +    GL+ +  
Sbjct: 131 RRGAGRNEGVPTLEGIIQQLVNGIIAPTAMSNLGVGPWGVLHSNPMDYAWGANGLDTIIT 190

Query: 189 QLSLNEQREPVPTPASHSCIEAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHI 248
           QL LN+     P PA    I+A+PTI+I + H+G    CPVCKE + +    + LPCNH+
Sbjct: 191 QL-LNQFENTGPPPADTDKIQALPTIQITEEHVGFGLECPVCKEDYTVGESVRQLPCNHL 250

Query: 249 YHNDCILPWLVQHNTCPVCRLELPQQESGHSWGGSGD 253
           +HNDCI+PWL QH+TCPVCR  L  Q +  +  G  D
Sbjct: 251 FHNDCIIPWLEQHDTCPVCRKSLSGQNTATNPPGLTD 286

BLAST of CSPI01G29460 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.9e-24
Identity = 89/304 (29.28%), Postives = 133/304 (43.75%), Query Frame = 1

Query: 10  WCHQCHHSFWLD----GEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEE----DLSTQ 69
           +C+QC+ +  +       D  CP C  GF+EE ++ +        NP        DL   
Sbjct: 24  FCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFLT 83

Query: 70  VPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFN 129
           +   F ++  L+        F    +  T +   +   NP+     RSG   G    F +
Sbjct: 84  LSDPFASLLPLL--------FPSSSSTTTSSSASIDPNNPSLSGPTRSGR--GDPFAF-D 143

Query: 130 SFWSFHDHM-----SGSTFANVTPEGRSSQH-------------TGLEELAAQLSLNEQR 189
            F    +H+     SG+    V     S Q               GLE+L  QL+ N+  
Sbjct: 144 PFTFIQNHLNDLRSSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPN 203

Query: 190 EPVPTPASHSCIEAMPTIKINQMHLGTD-SHCPVCKEKFELESEAKALPCNHIYHNDCIL 249
                PAS S IEA+P + I + +L ++ + C VC + FE  +EAK +PC H+YH DC+L
Sbjct: 204 RYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLL 263

Query: 250 PWLVQHNTCPVCRLELPQQE----------SGHSWGGSGDNNSEDLN-EREITQRNLRRR 276
           PWL  HN+CPVCR ELP  +           G S G  GDN+ +  + +    +R+ R  
Sbjct: 264 PWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRIS 316

BLAST of CSPI01G29460 vs. TrEMBL
Match: A0A0A0LZS8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600760 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 7.7e-189
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 1

Query: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60
           MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST
Sbjct: 59  MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 118

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 119 QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 178

Query: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 179 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 238

Query: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ
Sbjct: 239 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 298

Query: 241 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 300
           QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR
Sbjct: 299 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 358

Query: 301 DHDNEMGYPE 311
           DHDNEMGYPE
Sbjct: 359 DHDNEMGYPE 368

BLAST of CSPI01G29460 vs. TrEMBL
Match: W9QUU8_9ROSA (E3 ubiquitin-protein ligase RING1-like protein OS=Morus notabilis GN=L484_022532 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-66
Identity = 156/329 (47.42%), Postives = 196/329 (59.57%), Query Frame = 1

Query: 3   SFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEEL--NDEHDETVQNDFNPGIEEDLST 62
           S G DTHWC+QC  +F + G D+VCP+C GGFVEE+   + H  + +++F    + ++S 
Sbjct: 2   SSGGDTHWCYQCSRTFRIRGGDVVCPYCDGGFVEEICETEMHGLSRRDEFIS--DSEVSH 61

Query: 63  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 122
           Q+P IF+ MFALMGR  P  RF     +DT  R RMAGRNPNFDVR RSG      +   
Sbjct: 62  QMPDIFDLMFALMGRSGPDQRF-----IDTI-RHRMAGRNPNFDVRARSGPEHNWGVSGS 121

Query: 123 NSFWSFHDHMSGSTFANVTP-EGRSSQH-------TGLEELAAQLSLNEQREPVPTPASH 182
                F     G  F++ +P  GR            GLEEL  QL++N++R P   PAS 
Sbjct: 122 APLLIFQGQFPGLAFSHGSPGHGRRRADFGDYFVGPGLEELIGQLTMNDRRGP--PPASR 181

Query: 183 SCIEAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCP 242
           S IEAMPTIKI Q HL TD HCPVCKEKFEL SEA+ +PCNHIYH+DCI+PWLVQHN+CP
Sbjct: 182 SSIEAMPTIKITQAHLRTDLHCPVCKEKFELGSEAREMPCNHIYHSDCIVPWLVQHNSCP 241

Query: 243 VCRLELP-----QQESGHSW-GGSGDNNS----EDLNEREITQRNLRRRHPIPDFFAPRR 302
           VCR+ELP           SW GGS   +S    +    RE  ++N  RR+P+  F  P R
Sbjct: 242 VCRVELPPLGTSTSRGSRSWVGGSSSGSSSSGIDGSTSRETGRQNYGRRNPL-SFLWPFR 301

Query: 303 IV---TRFSSGIHINGLSSDRDHDNEMGY 309
                TR  S    N  S+ R+ +NEMGY
Sbjct: 302 SSNPNTRRDSETGGNSSSTTRERNNEMGY 319

BLAST of CSPI01G29460 vs. TrEMBL
Match: A0A151QZ96_CAJCA (RING finger protein 126 family OS=Cajanus cajan GN=KK1_043322 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.7e-66
Identity = 139/287 (48.43%), Postives = 184/287 (64.11%), Query Frame = 1

Query: 8   THWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLSTQVPPIFE 67
           THWC++C     LDG D+VCP+C  GF++EL++    T  + F+    E    Q+P IF+
Sbjct: 6   THWCYECRQPIVLDGRDVVCPYCDQGFIQELDELRGTTPHHPFSSQSGE--FHQMPDIFD 65

Query: 68  AMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPG--QNLDFFNS--F 127
           A+ A MG+R    RFGL++AVD F R RMAGRN NFDVR RSGS+P   Q+   F+S  +
Sbjct: 66  AIHAFMGQRGTDQRFGLMDAVDNFMRHRMAGRNSNFDVRGRSGSLPAPEQSWGVFSSGPY 125

Query: 128 WSFHDHMSGSTFANVTPEGRSSQ--------HTGLEELAAQLSLNEQREPVPTPASHSCI 187
             FH  + G T AN +P G   +          GLEEL  QL++N++R P   PA+ S I
Sbjct: 126 LIFHGQVPGFTLANGSPRGGPRRVDFGDYFMGPGLEELIEQLTMNDRRGP--PPAARSSI 185

Query: 188 EAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCR 247
           +AMP ++I Q HL +DSHCPVCKEKFEL +EA+ +PCNHIYH+DCI+PWLVQHN+CPVCR
Sbjct: 186 DAMPIVRITQAHLRSDSHCPVCKEKFELGTEAREMPCNHIYHSDCIVPWLVQHNSCPVCR 245

Query: 248 LELPQQ-----ESGHSWGGS--GDNNSEDLNE-REITQRNLRRRHPI 275
           +ELP Q         SWGG   G+N+S   N+     + N  RR+P+
Sbjct: 246 VELPPQGQVNSRGTRSWGGRNVGNNSSSSSNDSSRARESNHGRRNPL 288

BLAST of CSPI01G29460 vs. TrEMBL
Match: I1M784_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_14G039000 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.8e-66
Identity = 146/323 (45.20%), Postives = 189/323 (58.51%), Query Frame = 1

Query: 8   THWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLSTQVPPIFE 67
           THWC+ C     LDG D VCP+C GGFV+EL++       ++     +     Q+P IF+
Sbjct: 6   THWCYACRQPIVLDGRDPVCPYCDGGFVQELDELRGIAPNHNHTFSSQSGDFHQMPDIFD 65

Query: 68  AMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGS--VPGQNLDFFNS--F 127
           A+ A MG+R    RFGL++AVD F R RMAGRN NFDVR RSGS  VP Q+   ++S  +
Sbjct: 66  AIHAFMGQRGSDQRFGLMDAVDNFMRHRMAGRNSNFDVRGRSGSLPVPEQSWGVYSSGPY 125

Query: 128 WSFHDHMSGSTFANVTPEGRSSQ--------HTGLEELAAQLSLNEQREPVPTPASHSCI 187
             FH  + G T +  +P G   +          GLEEL  QL++N+QR P P  A+ S I
Sbjct: 126 LIFHGQVPGFTLSAGSPRGGPRRVDFGDYFMGPGLEELIEQLTMNDQRGPAP--AARSSI 185

Query: 188 EAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCR 247
           +AMPTIKI Q HL +DSHCPVCKEKFEL +EA+ +PCNHIYH+DCI+PWLVQHN+CPVCR
Sbjct: 186 DAMPTIKITQAHLRSDSHCPVCKEKFELGTEAREMPCNHIYHSDCIVPWLVQHNSCPVCR 245

Query: 248 LELPQQ-----ESGHSWGGSGDNNS------EDLNEREITQRNLRRRHPIPDFFAPRRIV 307
           +ELP Q         SWGG   +NS      +    RE + +N  RR+P    +  R   
Sbjct: 246 VELPPQGQASSRGTRSWGGRNASNSSSSGSNDSSRGRENSHQNHGRRNPFSFLWPFRSSN 305

BLAST of CSPI01G29460 vs. TrEMBL
Match: A0A0B2R254_GLYSO (E3 ubiquitin-protein ligase RING1-like OS=Glycine soja GN=glysoja_038229 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.8e-66
Identity = 146/323 (45.20%), Postives = 189/323 (58.51%), Query Frame = 1

Query: 8   THWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLSTQVPPIFE 67
           THWC+ C     LDG D VCP+C GGFV+EL++       ++     +     Q+P IF+
Sbjct: 6   THWCYACRQPIVLDGRDPVCPYCDGGFVQELDELRGIAPNHNHTFSSQSGDFHQMPDIFD 65

Query: 68  AMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGS--VPGQNLDFFNS--F 127
           A+ A MG+R    RFGL++AVD F R RMAGRN NFDVR RSGS  VP Q+   ++S  +
Sbjct: 66  AIHAFMGQRGSDQRFGLMDAVDNFMRHRMAGRNSNFDVRGRSGSLPVPEQSWGVYSSGPY 125

Query: 128 WSFHDHMSGSTFANVTPEGRSSQ--------HTGLEELAAQLSLNEQREPVPTPASHSCI 187
             FH  + G T +  +P G   +          GLEEL  QL++N+QR P P  A+ S I
Sbjct: 126 LIFHGQVPGFTLSAGSPRGGPRRVDFGDYFMGPGLEELIEQLTMNDQRGPAP--AARSSI 185

Query: 188 EAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCR 247
           +AMPTIKI Q HL +DSHCPVCKEKFEL +EA+ +PCNHIYH+DCI+PWLVQHN+CPVCR
Sbjct: 186 DAMPTIKITQAHLRSDSHCPVCKEKFELGTEAREMPCNHIYHSDCIVPWLVQHNSCPVCR 245

Query: 248 LELPQQ-----ESGHSWGGSGDNNS------EDLNEREITQRNLRRRHPIPDFFAPRRIV 307
           +ELP Q         SWGG   +NS      +    RE + +N  RR+P    +  R   
Sbjct: 246 VELPPQGQASSRGTRSWGGRNASNSSSSGSNDSSRGRENSHQNHGRRNPFSFLWPFRSSN 305

BLAST of CSPI01G29460 vs. TAIR10
Match: AT3G56580.2 (AT3G56580.2 RING/U-box superfamily protein)

HSP 1 Score: 174.1 bits (440), Expect = 1.3e-43
Identity = 112/293 (38.23%), Postives = 142/293 (48.46%), Query Frame = 1

Query: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60
           MSS   +THWCH+C  + WL   D VC +C GGFVEE+ D        D    +E D   
Sbjct: 1   MSSI-RNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRD----VERD--- 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
                              P F L+EA   F R R+A R+ + ++  R GS   ++    
Sbjct: 61  -------------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNL 120

Query: 121 NSFWSF----------HDHMSGSTFANVTPEGRSSQH----------TGLEELAAQLSLN 180
                F           D+ S   F N    G                GLEEL  QLS  
Sbjct: 121 APLLIFGGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSG 180

Query: 181 EQREPVPTPASHSCIEAMPTIKINQMHL-GTDSHCPVCKEKFELESEAKALPCNHIYHND 240
                 P PA  S I+A+PTIKI Q HL  +DSHCPVCK++FEL+SEAK +PC+HIYH+D
Sbjct: 181 THHRG-PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSD 240

Query: 241 CILPWLVQHNTCPVCRLELPQQESGHSWGGSGDNNSEDLNEREITQRNLRRRH 273
           CI+PWLVQHN+CPVCR ELP +       GS  +     N     + N RRR+
Sbjct: 241 CIVPWLVQHNSCPVCRKELPSR-------GSSSSTQSSQNRSTNGRENSRRRN 257

BLAST of CSPI01G29460 vs. TAIR10
Match: AT2G40830.2 (AT2G40830.2 RING-H2 finger C1A)

HSP 1 Score: 168.7 bits (426), Expect = 5.7e-42
Identity = 119/331 (35.95%), Postives = 163/331 (49.24%), Query Frame = 1

Query: 3   SFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLSTQV 62
           S   +THWCH+C  +  L G++ VC +C GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSF---------HDHMSGSTFANVTP-----EGRSSQH---TGLEELAAQLSLNEQREP 182
              F          D+   + F   +P      G +  +    GLEEL  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPTPASHSCIEAMPTIKINQMHL-GTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPW 242
            P PA  S I+A+PTIKI Q HL  +DS+CPVCK++FEL SEAK +PCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELP--------QQESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPD 302
           LVQHN+CPVCR ELP        Q  +  +      ++S   N RE       RR+P   
Sbjct: 242 LVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFSS 301

Query: 303 FFAPRRIVTRFSS----GIHINGLSSDRDHD 304
           F+  R   +  SS    G   N  +SD +H+
Sbjct: 302 FWPFRSSGSSSSSTQNRGGTRNSDTSDENHN 308

BLAST of CSPI01G29460 vs. TAIR10
Match: AT5G59550.1 (AT5G59550.1 zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 130.2 bits (326), Expect = 2.2e-30
Identity = 88/273 (32.23%), Postives = 127/273 (46.52%), Query Frame = 1

Query: 8   THWCHQCHH--SFWLDGE-----DIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 67
           ++WC+ C    S W D        + CPHC GGF+E++ND      +             
Sbjct: 17  SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATE------------L 76

Query: 68  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFD--------VRRRSGSV 127
            +P   E       RRS               R R +GR P+F+           R    
Sbjct: 77  TIPASTEVRSINNNRRS-------------VIRRRRSGRRPSFNPVIVLQGGAGEREEGE 136

Query: 128 PGQNLDFFNSFWSFHDHMSGSTFANVTPEGRSS--QHTGLEELAAQLSLNEQ------RE 187
            G       +F  ++D  SGS    + P+  S     +G E L  QLS  E       R 
Sbjct: 137 EGDAARDRRAFEFYYDDGSGSGLRPL-PDSVSEILMGSGFERLLEQLSQIEASATGIGRS 196

Query: 188 PVPTPASHSCIEAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPW 247
             P PAS S IE++P ++I+  H+G++++C VC E FE E+EA+ +PC H++H+DCI+PW
Sbjct: 197 GNP-PASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPW 256

Query: 248 LVQHNTCPVCRLELPQQESGHSWGGSGDNNSED 258
           L   N+CPVCR ELP + +  S     +NN ED
Sbjct: 257 LSIRNSCPVCRFELPSEPNRRS-----NNNEED 257

BLAST of CSPI01G29460 vs. TAIR10
Match: AT2G39720.1 (AT2G39720.1 RING-H2 finger C2A)

HSP 1 Score: 125.6 bits (314), Expect = 5.5e-29
Identity = 86/256 (33.59%), Postives = 123/256 (48.05%), Query Frame = 1

Query: 8   THWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGI--EEDLSTQVPPI 67
           ++WC+ C    W+  + I CP C GGF+E + +  D T  + F      +    T+ PP 
Sbjct: 5   SYWCYSCSRFVWVS-DSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRFPPP 64

Query: 68  FEAMF----ALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRR------SGSVPGQ 127
             +      ++    SP P         T      + R+PN  +  R      S  V  +
Sbjct: 65  SSSSSTPSASMHADNSPTP---------TIVTRTRSNRSPNPVIVLRGSAAAPSSDVVSE 124

Query: 128 NLDFFNSFWSFHDHMSGSTFANVTPEGRS----SQHTGLEELAAQLSLNEQREPVPT--- 187
            LD  ++F  ++D  + S    + P        S    L +  +Q+ LN  R        
Sbjct: 125 GLDR-SAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHP 184

Query: 188 PASHSCIEAMPTIKINQMHL--GTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLV 243
           PAS S IEA+P I+I+  HL   + SHC VCKE F L+S A+ +PCNHIYH DCILPWL 
Sbjct: 185 PASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLA 244

BLAST of CSPI01G29460 vs. TAIR10
Match: AT3G10815.1 (AT3G10815.1 RING/U-box superfamily protein)

HSP 1 Score: 115.9 bits (289), Expect = 4.4e-26
Identity = 51/98 (52.04%), Postives = 68/98 (69.39%), Query Frame = 1

Query: 146 QHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPTIKINQMHLGTDSHCPVCKEKFELES 205
           +    EEL  +L   + R   P PAS + I ++  IKI Q HLG D +CPVC+++FE+ S
Sbjct: 74  EQLSFEELFNRLPALQDRRG-PPPASLAAINSLQKIKIRQKHLGLDPYCPVCQDQFEIGS 133

Query: 206 EAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQQES 244
           +A+ +PC HIYH++CILPWLVQ NTCPVCR ELPQ  +
Sbjct: 134 DARKMPCKHIYHSECILPWLVQRNTCPVCRKELPQDRN 170

BLAST of CSPI01G29460 vs. NCBI nr
Match: gi|449452702|ref|XP_004144098.1| (PREDICTED: E3 ubiquitin-protein ligase RNF126-B-like [Cucumis sativus])

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-188
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 1

Query: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60
           MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST
Sbjct: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 300
           QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR
Sbjct: 241 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 300

Query: 301 DHDNEMGYPE 311
           DHDNEMGYPE
Sbjct: 301 DHDNEMGYPE 310

BLAST of CSPI01G29460 vs. NCBI nr
Match: gi|700211291|gb|KGN66387.1| (hypothetical protein Csa_1G600760 [Cucumis sativus])

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-188
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 1

Query: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60
           MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST
Sbjct: 59  MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 118

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 119 QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 178

Query: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 179 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 238

Query: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ
Sbjct: 239 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 298

Query: 241 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 300
           QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR
Sbjct: 299 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 358

Query: 301 DHDNEMGYPE 311
           DHDNEMGYPE
Sbjct: 359 DHDNEMGYPE 368

BLAST of CSPI01G29460 vs. NCBI nr
Match: gi|659100327|ref|XP_008451039.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 637.9 bits (1644), Expect = 9.4e-180
Identity = 293/310 (94.52%), Postives = 303/310 (97.74%), Query Frame = 1

Query: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60
           MSS GEDTHWC+QC+HSFWLDGED+VCPHC GGFVEELND+HDET+QNDFNPGIEEDLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTF NVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFELESEAKALPCNHIYH DCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 300
           QESGHSWGG+G +NSEDLNERE+TQRNLRRRHP PDFFAPRRIVTRF SGIHINGLSSDR
Sbjct: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300

Query: 301 DHDNEMGYPE 311
           DHDNEMGYPE
Sbjct: 301 DHDNEMGYPE 310

BLAST of CSPI01G29460 vs. NCBI nr
Match: gi|1012202077|ref|XP_015973474.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Arachis duranensis])

HSP 1 Score: 265.8 bits (678), Expect = 9.7e-68
Identity = 143/289 (49.48%), Postives = 178/289 (61.59%), Query Frame = 1

Query: 8   THWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLSTQVPPIFE 67
           THWC++C     L G D+VCP+C GGFV+ELN+      Q+ F P  +     Q+P  F+
Sbjct: 6   THWCYECRQPIVLGGRDVVCPYCDGGFVQELNELQGFAPQHAF-PSSQMGEFHQMPDFFD 65

Query: 68  AMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDF----FNSF 127
           A+ A MGRR P PRFG ++AVD F R+RMAGR+PNFDVR RSG  PG    +       +
Sbjct: 66  ALHAFMGRRGPDPRFGFIDAVDNFMRQRMAGRHPNFDVRGRSGPAPGPEQSWGVVSSGPY 125

Query: 128 WSFHDHMSGSTFANV-TPEGRSS--------QHTGLEELAAQLSLNEQREPVPTPASHSC 187
             FH  + G T AN  +P G              GLEEL  QL++NE+R P   PA+ S 
Sbjct: 126 LIFHGQVPGFTLANGGSPRGGPRGVDFGDYFMGPGLEELIEQLTMNERRGP--PPAARSS 185

Query: 188 IEAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVC 247
           I+AMPTIKI   HL  DSHCPVCKEKFEL SEA+ +PCNHIYH DCI+PWLVQHN+CPVC
Sbjct: 186 IDAMPTIKITSAHLRQDSHCPVCKEKFELGSEAREMPCNHIYHTDCIVPWLVQHNSCPVC 245

Query: 248 RLELPQQ-----ESGHSWGGSGDNNSEDLN----EREITQRNLRRRHPI 275
           R+ELP Q         S GG   N+S D +     RE + ++  RRHP+
Sbjct: 246 RVELPPQGHVSSRGSRSGGGRNANSSGDSDSSSRSRESSHQSQGRRHPL 291

BLAST of CSPI01G29460 vs. NCBI nr
Match: gi|1021488476|ref|XP_016187219.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Arachis ipaensis])

HSP 1 Score: 261.9 bits (668), Expect = 1.4e-66
Identity = 141/289 (48.79%), Postives = 178/289 (61.59%), Query Frame = 1

Query: 8   THWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLSTQVPPIFE 67
           THWC++C     L G D+VCP+C GGFV+ELN+      Q+ F P  +     Q+P  F+
Sbjct: 6   THWCYECRQPIVLGGRDVVCPYCDGGFVQELNELQGFAPQHAF-PSSQMGEFHQMPDFFD 65

Query: 68  AMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDF----FNSF 127
           A+ A MGRR P PRFG ++AVD F R+RMAGR+PNFDVR RSG  PG    +       +
Sbjct: 66  ALHAFMGRRGPDPRFGFIDAVDNFMRQRMAGRHPNFDVRGRSGPAPGPEQSWGVVSSGPY 125

Query: 128 WSFHDHMSGSTFANV-TPEGRSS--------QHTGLEELAAQLSLNEQREPVPTPASHSC 187
             FH  + G T AN  +P G              GLEEL  QL++NE+R P   PA+ + 
Sbjct: 126 LIFHGQVPGFTLANGGSPRGGPRGVDFGDYFMGPGLEELIEQLTMNERRGP--PPAARTS 185

Query: 188 IEAMPTIKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVC 247
           I+AMPTIKI   HL  DSHCPVCKEKFEL SEA+ +PCNHIYH+DCI+PWLVQHN+CPVC
Sbjct: 186 IDAMPTIKITSAHLRQDSHCPVCKEKFELGSEAREMPCNHIYHSDCIVPWLVQHNSCPVC 245

Query: 248 RLELPQQ-----ESGHSWGGSGDNNSED----LNEREITQRNLRRRHPI 275
           R+ELP Q         S GG   N+S D       RE + ++  RR+P+
Sbjct: 246 RVELPPQGHASSRGSRSGGGRNANSSGDSESSSRSRESSHQSQGRRNPL 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RHC1A_ARATH1.0e-4035.95Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2... [more]
RDUF2_ARATH4.0e-2932.23E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1[more]
RHC2A_ARATH9.8e-2833.59Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana GN=RHC2A PE=2... [more]
R126B_XENLA1.2e-2528.52E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis GN=rnf126-b PE=2 SV=1[more]
RING1_GOSHI2.9e-2429.28E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LZS8_CUCSA7.7e-189100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600760 PE=4 SV=1[more]
W9QUU8_9ROSA1.7e-6647.42E3 ubiquitin-protein ligase RING1-like protein OS=Morus notabilis GN=L484_022532... [more]
A0A151QZ96_CAJCA3.7e-6648.43RING finger protein 126 family OS=Cajanus cajan GN=KK1_043322 PE=4 SV=1[more]
I1M784_SOYBN4.8e-6645.20Uncharacterized protein OS=Glycine max GN=GLYMA_14G039000 PE=4 SV=1[more]
A0A0B2R254_GLYSO4.8e-6645.20E3 ubiquitin-protein ligase RING1-like OS=Glycine soja GN=glysoja_038229 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT3G56580.21.3e-4338.23 RING/U-box superfamily protein[more]
AT2G40830.25.7e-4235.95 RING-H2 finger C1A[more]
AT5G59550.12.2e-3032.23 zinc finger (C3HC4-type RING finger) family protein[more]
AT2G39720.15.5e-2933.59 RING-H2 finger C2A[more]
AT3G10815.14.4e-2652.04 RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449452702|ref|XP_004144098.1|1.1e-188100.00PREDICTED: E3 ubiquitin-protein ligase RNF126-B-like [Cucumis sativus][more]
gi|700211291|gb|KGN66387.1|1.1e-188100.00hypothetical protein Csa_1G600760 [Cucumis sativus][more]
gi|659100327|ref|XP_008451039.1|9.4e-18094.52PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo][more]
gi|1012202077|ref|XP_015973474.1|9.7e-6849.48PREDICTED: E3 ubiquitin-protein ligase RING1-like [Arachis duranensis][more]
gi|1021488476|ref|XP_016187219.1|1.4e-6648.79PREDICTED: E3 ubiquitin-protein ligase RING1-like [Arachis ipaensis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0005515protein binding
GO:0008270zinc ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
cellular_component GO:0005575 cellular_component
cellular_component GO:0005618 cell wall
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0061630 ubiquitin protein ligase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G29460.1CSPI01G29460.1mRNA
CSPI01G29460.2CSPI01G29460.2mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 193..235
score: 8.8
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 194..234
score: 1.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 194..235
score: 12
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 191..239
score: 3.8
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 6..271
score: 1.8
NoneNo IPR availablePANTHERPTHR22763:SF44E3 UBIQUITIN LIGASEcoord: 6..271
score: 1.8
NoneNo IPR availablePFAMPF14369zinc_ribbon_9coord: 8..38
score: 6.
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 174..242
score: 7.07