CSPI01G02460 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G02460
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Invertase/pectin methylesterase inhibitor family protein
Location	Chr1 : 1556646 .. 1557278 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAAAACATCCTTCTTCCTAAAACTATTAGCCAAAGAAATAATAAAAAAAAAAGTTATGATGATCATGATCTCTACTTCCTTTCTCTTCTCCTTCACTATTGTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACACCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGACTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAAAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGTAAGTTCAATTATGGAAGCTCCTACAACTTGTGAAAATGGGTTTAAAGAAAAAGAAGGAATCATTTCGCCATTGACAAAGAATAACAGTGATGTGTTTCAATTGGCAGCTTTGACTCTCTCCATCATCAATATGAATCTGCATGATATCCAATAA

mRNA sequence

ATGATGATCATGATCTCTACTTCCTTTCTCTTCTCCTTCACTATTGTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACACCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGACTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAAAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGTAAGTTCAATTATGGAAGCTCCTACAACTTGTGAAAATGGGTTTAAAGAAAAAGAAGGAATCATTTCGCCATTGACAAAGAATAACAGTGATGTGTTTCAATTGGCAGCTTTGACTCTCTCCATCATCAATATGAATCTGCATGATATCCAATAA

Coding sequence (CDS)

ATGATGATCATGATCTCTACTTCCTTTCTCTTCTCCTTCACTATTGTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACACCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGACTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAAAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGTAAGTTCAATTATGGAAGCTCCTACAACTTGTGAAAATGGGTTTAAAGAAAAAGAAGGAATCATTTCGCCATTGACAAAGAATAACAGTGATGTGTTTCAATTGGCAGCTTTGACTCTCTCCATCATCAATATGAATCTGCATGATATCCAATAA

Homology

[Close]

BLAST of CSPI01G02460 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.5e-29
Identity = 65/153 (42.48%), Postives = 106/153 (69.28%), Query Frame = 1


Query: 33  NIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIK 92
           +I+Q TCKK A  +PN+ Y FCV SL +D +S  A+L  LG+IS +L   + +  +  I 
Sbjct: 25  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIG 84

Query: 93  KLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCE 152
           ++L++K ++  +K  L DCV LY+DA  +++EA  ++K+++Y  AN+K+S+ ++   TCE
Sbjct: 85  RILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCE 144

Query: 153 NGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           +GFKEK+GI+SP+TK N D  QL A++L+I  +
Sbjct: 145 DGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of CSPI01G02460 vs. Swiss-Prot
Match: PMEI_ACTDE (Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 6.3e-09
Identity = 56/187 (29.95%), Postives = 93/187 (49.73%), Query Frame = 1


Query: 1   MMIMISTSFLFSFTIVFILIFSSFSQTYS--NPHNIIQETCKKSAASTPNLTYKFCVTSL 60
           M     +S LF   ++ IL  S  SQ  S    +++I E C K+    P+L    C+ +L
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTR--NPSL----CLQAL 60

Query: 61  ESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDA 120
           ESD RS   +L  LG  S+D+ + +   T + I  L  N+  +  +KG  + C E Y+DA
Sbjct: 61  ESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIASLT-NQATDPKLKGRYETCSENYADA 120

Query: 121 VPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAAL 180
           + +L +AK+   + +Y   NI  S+  +   TCE+ F+    I + L + +  +  L  +
Sbjct: 121 IDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDI 180

Query: 181 TLSIINM 186
            L I N+
Sbjct: 181 VLVISNL 180

[Close]

BLAST of CSPI01G02460 vs. Swiss-Prot
Match: CVIF2_ARATH (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.3e-09
Identity = 48/161 (29.81%), Postives = 78/161 (48.45%), Query Frame = 1


Query: 5   ISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRS 64
           +++S +F   +      S+     SN   II+ TCK     T N  YKFCV++L+SD RS
Sbjct: 1   MASSLIFLLLVTLTFSASTLISAKSNTTTIIESTCK-----TTNY-YKFCVSALKSDPRS 60

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
             A+   L  I + +   N TST   I   L     +  +K  L+DC E Y+ A  +L+ 
Sbjct: 61  PTADTKGLASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADSLRL 120

Query: 125 AKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPL 166
             ++  +  Y  A++ V +  + P  C N F+  +G+  P+
Sbjct: 121 TIQDLDDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPV 155

[Close]

BLAST of CSPI01G02460 vs. Swiss-Prot
Match: PMEI2_ARATH (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.5e-07
Identity = 37/133 (27.82%), Postives = 68/133 (51.13%), Query Frame = 1


Query: 53  FCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCV 112
           FC + ++S+ ++  A+L  L  I+    + + +   R+I+ L++       +K     CV
Sbjct: 42  FCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTAT-NPTMKKAYTSCV 101

Query: 113 ELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDV 172
           + Y  A+ +L +AK+   + + K  NIKVS+ ME P+TCE    + + +     KN+ D 
Sbjct: 102 QHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFK-VDPSAVKNSGDF 161

Query: 173 FQLAALTLSIINM 186
             +  + L I NM
Sbjct: 162 QNICGIVLVISNM 172

[Close]

BLAST of CSPI01G02460 vs. TrEMBL
Match: A0A0A0LUJ3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G009900 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 1.9e-97
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 1


Query: 4   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 63
           MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR
Sbjct: 1   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 60

Query: 64  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 123
           SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK
Sbjct: 61  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 120

Query: 124 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 183
           EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII
Sbjct: 121 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 180

Query: 184 NMNLHDIQ 192
           NMNLHDIQ
Sbjct: 181 NMNLHDIQ 188

[Close]

BLAST of CSPI01G02460 vs. TrEMBL
Match: B9MVC0_POPTR (Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa GN=POPTR_0014s04360g PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.7e-48
Identity = 95/176 (53.98%), Postives = 140/176 (79.55%), Query Frame = 1


Query: 11  FSFTIVFILIFSSFSQTYSN-PHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANL 70
           FS+ ++ + +   F   ++N   N+I ETCKK AA+ PNL+Y FCVTSL++  RS+  NL
Sbjct: 4   FSYCLLLLFLPLLFCTFHANIAQNLIPETCKKCAANDPNLSYNFCVTSLQASNRSQCDNL 63

Query: 71  HKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREY 130
             LG++S+ L+++NVT+TR  +K LL+NKKM+ FI+ CL DC++LYSDA+PTLK+A  +Y
Sbjct: 64  RGLGMMSIKLIKYNVTNTRHYVKNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMIDY 123

Query: 131 KNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           K+++YKDANI+VSS+++A TTCE+GF++KEG +SPLTK N+D FQL+A+ L++INM
Sbjct: 124 KSKHYKDANIEVSSVIDAATTCEDGFEDKEGAVSPLTKRNNDTFQLSAIALALINM 179

[Close]

BLAST of CSPI01G02460 vs. TrEMBL
Match: A0A067HCB9_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g036528mg PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 5.3e-47
Identity = 92/154 (59.74%), Postives = 123/154 (79.87%), Query Frame = 1


Query: 32  HNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREI 91
           HNI+ ETCKK A + PN+++ FCVTSL++D +S+ ANL +LG+ISM L R NVT TR  I
Sbjct: 17  HNIVHETCKKCAQNDPNISHNFCVTSLQADAKSQCANLGELGMISMKLTRENVTKTRSYI 76

Query: 92  KKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTC 151
           K LL+ KK++ F++ CL DC +LYSDAVPTLK+A ++YK + Y+DANI VSS+++A TTC
Sbjct: 77  KDLLKKKKLDPFMRACLHDCFDLYSDAVPTLKQAMKDYKAKQYEDANIGVSSVVDACTTC 136

Query: 152 ENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           E+GFKE +G +SPLTK N D FQL+ ++LSIINM
Sbjct: 137 EDGFKENQGAVSPLTKRNYDAFQLSVISLSIINM 170

[Close]

BLAST of CSPI01G02460 vs. TrEMBL
Match: V4U3U3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023652mg PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 7.6e-46
Identity = 98/176 (55.68%), Postives = 129/176 (73.30%), Query Frame = 1


Query: 10  LFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANL 69
           LF     F L   SF       HNI+ ETCKK A + PN+++ FCVTSL++D +S+ ANL
Sbjct: 12  LFLSLFFFFLFVCSFHGITG--HNIVHETCKKCAQNDPNISHNFCVTSLQADAKSQCANL 71

Query: 70  HKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREY 129
            +LG+ISM L R NVT TR  IK LL+ KK++ F++ CL DC +LYSDAVPTLK+A ++Y
Sbjct: 72  GELGMISMKLTRENVTKTRSYIKDLLKKKKLDPFMRACLHDCFDLYSDAVPTLKQAMKDY 131

Query: 130 KNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           K + Y+DANI VSS+++A TTCE+GFKE +G +SPLTK N D FQL+ ++LSIINM
Sbjct: 132 KAKQYEDANIGVSSVVDACTTCEDGFKENQGAVSPLTKRNYDAFQLSVISLSIINM 185

[Close]

BLAST of CSPI01G02460 vs. TrEMBL
Match: A0A0D2PD74_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G168200 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 7.1e-44
Identity = 98/186 (52.69%), Postives = 137/186 (73.66%), Query Frame = 1


Query: 5   ISTSFLFSFTIVFILIFSSFSQTY---SNPHNIIQETCKKSAASTPNLTYKFCVTSLESD 64
           +S+SF+    +     F S S ++   +   ++I++TCKK A S PNL+Y FC+TSL++ 
Sbjct: 5   LSSSFVIPLFLCIFFFFVSISTSHGLTATNDSLIRKTCKKCAQSDPNLSYNFCITSLQAA 64

Query: 65  TRSRYAN-LHKLGLISMDLLRHNVTSTRREIKKLLRN-KKMEEFIKGCLKDCVELYSDAV 124
             S  AN L +LG IS+ LL  NVT+TR  IK+LL+N KKM+ F++ CL DC +LYSDA+
Sbjct: 65  PHSHCANDLRQLGKISITLLGRNVTNTRSHIKELLKNQKKMDPFVRSCLHDCFDLYSDAI 124

Query: 125 PTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALT 184
           PT K+A ++YK ++Y DANI VSS+M+A TTCE+GFKEKEG++SPLTK N+D F L+A++
Sbjct: 125 PTTKQALQDYKAKHYDDANIDVSSVMDATTTCEDGFKEKEGLVSPLTKRNNDAFMLSAIS 184

Query: 185 LSIINM 186
           LSIINM
Sbjct: 185 LSIINM 190

[Close]

BLAST of CSPI01G02460 vs. TAIR10
Match: AT5G46970.1 (AT5G46970.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-24
Identity = 66/168 (39.29%), Postives = 104/168 (61.90%), Query Frame = 1


Query: 8   SFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA 67
           +FLF F + F+L+       ++   ++IQ+ CKK+A   P + Y FCV SLE + +S+ A
Sbjct: 2   TFLFYFAMFFLLL-----NAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTA 61

Query: 68  -NLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAK 127
            +L +L ++S        TS +  + K+L+  + E + +  L+DC+ELYSDA  +LKEA 
Sbjct: 62  RSLDRLVMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEAL 121

Query: 128 REYKNRNYKDANIKVSSIMEAPTTCENGFKE-KEGIISPLTKNNSDVF 174
              K+R+YK AN+ +S+ M AP +CE GFKE K+ + SP TK+N  +F
Sbjct: 122 TIIKSRDYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of CSPI01G02460 vs. TAIR10
Match: AT5G46940.1 (AT5G46940.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 106.7 bits (265), Expect = 1.6e-23
Identity = 57/172 (33.14%), Postives = 104/172 (60.47%), Query Frame = 1


Query: 15  IVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA-NLHKLG 74
           +++++ F   S   +N   +I+ +CKK+ A++P   Y  CVTSLE++ +++ A +L  L 
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLV 63

Query: 75  LISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRN 134
           + S        T+ +  + K+++ KK+ +     L+DC++LY+DA+ +L EA    K+RN
Sbjct: 64  MASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRN 123

Query: 135 YKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           Y      +S+ M+ P+TCE GFKE++   SP+TK N +++Q+  + L+  NM
Sbjct: 124 YPTVKTVLSAAMDTPSTCETGFKERKA-PSPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of CSPI01G02460 vs. TAIR10
Match: AT5G46960.1 (AT5G46960.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 95.5 bits (236), Expect = 3.8e-20
Identity = 58/172 (33.72%), Postives = 99/172 (57.56%), Query Frame = 1


Query: 15  IVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA-NLHKLG 74
           +V ++IFS F   ++    +IQ++CKK+ A  P L+Y FCV SL  D +S+ A  L  L 
Sbjct: 4   LVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLV 63

Query: 75  LISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRN 134
           L S       +T+ +  + + L++++ ++ ++  LK C+  Y+DA   L  A    K+R+
Sbjct: 64  LASTKTAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRD 123

Query: 135 YKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           Y+ ANI +S+ ++ P  CE+ FKE +   SP+T  NS +F+   + L+  NM
Sbjct: 124 YQGANINLSAALDVPGNCEDDFKEAKK-TSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of CSPI01G02460 vs. TAIR10
Match: AT5G46930.1 (AT5G46930.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-19
Identity = 57/175 (32.57%), Postives = 101/175 (57.71%), Query Frame = 1


Query: 13  FTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA-NLHK 72
           + ++F L+ + F+   S    +I+++CKK+    P L Y FCV SLE + +S+ A +L  
Sbjct: 6   YLVMFFLLLNCFATAQS----LIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEG 65

Query: 73  LGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKN 132
           L  +S        TS +  + K+L+  K E  ++  L DC+ELY++A+ +L ++    K+
Sbjct: 66  LVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAIDSLNQSLDTVKS 125

Query: 133 RNYKDANIKVSSIMEAPTTCENGF-KEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           R+YK A + +S+ M+AP +CE  F K K+ + SP TK N  +F +  + ++  +M
Sbjct: 126 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of CSPI01G02460 vs. TAIR10
Match: AT5G38610.1 (AT5G38610.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 91.3 bits (225), Expect = 7.1e-19
Identity = 67/185 (36.22%), Postives = 96/185 (51.89%), Query Frame = 1


Query: 16  VFILIFSSF--SQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYA--NLHK 75
           VF +IF  F  SQ  S   ++I +TCK  AA +    Y FCV+SL +   +  +  NL  
Sbjct: 6   VFYIIFLFFLVSQVKSTS-DMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSS 65

Query: 76  LGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKN 135
           L L+ M     N T+T   I++LL +     F   CL+DC+ELY DA   L+EA R +  
Sbjct: 66  LALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFIT 125

Query: 136 R-NYKDANIKVSSIMEAPTTCENGFKEKE-------GI---ISPLTKNNSDVFQLAALTL 186
           R      N+ VS+ ME+  TCENGF+E++       G+    SP+   N  +F+   + L
Sbjct: 126 RKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQIAL 185

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: gi|778664693|ref|XP_011660336.1| (PREDICTED: putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 367.1 bits (941), Expect = 1.9e-98
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 1


Query: 1   MMIMISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLES 60
           MMIMISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLES
Sbjct: 1   MMIMISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLES 60

Query: 61  DTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVP 120
           DTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVP
Sbjct: 61  DTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVP 120

Query: 121 TLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTL 180
           TLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTL
Sbjct: 121 TLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTL 180

Query: 181 SIINMNLHDIQ 192
           SIINMNLHDIQ
Sbjct: 181 SIINMNLHDIQ 191

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: gi|700208590|gb|KGN63686.1| (hypothetical protein Csa_1G009900 [Cucumis sativus])

HSP 1 Score: 363.2 bits (931), Expect = 2.7e-97
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 1


Query: 4   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 63
           MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR
Sbjct: 1   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 60

Query: 64  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 123
           SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK
Sbjct: 61  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 120

Query: 124 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 183
           EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII
Sbjct: 121 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 180

Query: 184 NMNLHDIQ 192
           NMNLHDIQ
Sbjct: 181 NMNLHDIQ 188

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: gi|659107450|ref|XP_008453678.1| (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 267.7 bits (683), Expect = 1.6e-68
Identity = 134/142 (94.37%), Postives = 139/142 (97.89%), Query Frame = 1


Query: 50  TYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLK 109
           +YKFCVTSLESD RSR ANLHKLGLIS+DLLRHNVTSTRREIKKLLRNKKMEEFIKGCLK
Sbjct: 59  SYKFCVTSLESDRRSRSANLHKLGLISIDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLK 118

Query: 110 DCVELYSDAVPTLKEAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNN 169
           DCVELYSDAVPTLKEAKREYKNRNYKD+NIKVSSIMEAPTTCENGFKEKEGI+SPLT NN
Sbjct: 119 DCVELYSDAVPTLKEAKREYKNRNYKDSNIKVSSIMEAPTTCENGFKEKEGIVSPLTNNN 178

Query: 170 SDVFQLAALTLSIINMNLHDIQ 192
           S+VFQLAALTLSIINMNLHDIQ
Sbjct: 179 SNVFQLAALTLSIINMNLHDIQ 200

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: gi|743787742|ref|XP_011031708.1| (PREDICTED: putative invertase inhibitor [Populus euphratica])

HSP 1 Score: 200.7 bits (509), Expect = 2.4e-48
Identity = 96/176 (54.55%), Postives = 139/176 (78.98%), Query Frame = 1


Query: 11  FSFTIVFILIFSSFSQTYSNP-HNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANL 70
           FS+ ++ + +   FS  ++N   N+I ETCKK AA+ PNL+Y FCVTSL++  RS+  NL
Sbjct: 4   FSYCLLLLFLPLLFSTFHANTAQNLIPETCKKCAANDPNLSYNFCVTSLQASNRSQCDNL 63

Query: 71  HKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREY 130
             LG++S+ L++ NVT+TR  +K LL+NKKM+ FI+ CL DC++LYSDA+PTLK+A  +Y
Sbjct: 64  RGLGMMSIKLIKDNVTNTRNYVKSLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMMDY 123

Query: 131 KNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           K+++YKDANI+V S+++A TTCE+GF+EKEG +SPLTK N+D FQL+A+ L++INM
Sbjct: 124 KSKHYKDANIEVGSVIDAATTCEDGFEEKEGAVSPLTKRNNDTFQLSAIALALINM 179

[Close]

BLAST of CSPI01G02460 vs. NCBI nr
Match: gi|566202453|ref|XP_006375099.1| (invertase/pectin methylesterase inhibitor family protein [Populus trichocarpa])

HSP 1 Score: 199.5 bits (506), Expect = 5.3e-48
Identity = 95/176 (53.98%), Postives = 140/176 (79.55%), Query Frame = 1


Query: 11  FSFTIVFILIFSSFSQTYSN-PHNIIQETCKKSAASTPNLTYKFCVTSLESDTRSRYANL 70
           FS+ ++ + +   F   ++N   N+I ETCKK AA+ PNL+Y FCVTSL++  RS+  NL
Sbjct: 4   FSYCLLLLFLPLLFCTFHANIAQNLIPETCKKCAANDPNLSYNFCVTSLQASNRSQCDNL 63

Query: 71  HKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREY 130
             LG++S+ L+++NVT+TR  +K LL+NKKM+ FI+ CL DC++LYSDA+PTLK+A  +Y
Sbjct: 64  RGLGMMSIKLIKYNVTNTRHYVKNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMIDY 123

Query: 131 KNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSIINM 186
           K+++YKDANI+VSS+++A TTCE+GF++KEG +SPLTK N+D FQL+A+ L++INM
Sbjct: 124 KSKHYKDANIEVSSVIDAATTCEDGFEDKEGAVSPLTKRNNDTFQLSAIALALINM 179

The following BLAST results are available for this feature:

BLAST of CSPI01G02460 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLA1_PLAAC	5.5e-29	42.48	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]
PMEI_ACTDE	6.3e-09	29.95	Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2	[more]
CVIF2_ARATH	6.3e-09	29.81	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VI...	[more]
PMEI2_ARATH	1.5e-07	27.82	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1	[more]

BLAST of CSPI01G02460 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUJ3_CUCSA	1.9e-97	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G009900 PE=4 SV=1	[more]
B9MVC0_POPTR	3.7e-48	53.98	Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa ...	[more]
A0A067HCB9_CITSI	5.3e-47	59.74	Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g036528mg PE=4 S...	[more]
V4U3U3_9ROSI	7.6e-46	55.68	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023652mg PE=4 SV=1	[more]
A0A0D2PD74_GOSRA	7.1e-44	52.69	Uncharacterized protein OS=Gossypium raimondii GN=B456_004G168200 PE=4 SV=1	[more]

BLAST of CSPI01G02460 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G46970.1	1.1e-24	39.29	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	1.6e-23	33.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	3.8e-20	33.72	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	1.4e-19	32.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	7.1e-19	36.22	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of CSPI01G02460 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778664693\|ref\|XP_011660336.1\|	1.9e-98	100.00	PREDICTED: putative invertase inhibitor [Cucumis sativus]	[more]
gi\|700208590\|gb\|KGN63686.1\|	2.7e-97	100.00	hypothetical protein Csa_1G009900 [Cucumis sativus]	[more]
gi\|659107450\|ref\|XP_008453678.1\|	1.6e-68	94.37	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
gi\|743787742\|ref\|XP_011031708.1\|	2.4e-48	54.55	PREDICTED: putative invertase inhibitor [Populus euphratica]	[more]
gi\|566202453\|ref\|XP_006375099.1\|	5.3e-48	53.98	invertase/pectin methylesterase inhibitor family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G02460.1	CSPI01G02460.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 31..185 score: 1.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..182 score: 9.9
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..182 score: 3.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 8..185 score: 3.4
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..185 score: 5.23
None	No IPR available	unknown	Coil	Coil	coord: 77..97 scor
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 1..185 score: 1.7
None	No IPR available	PANTHER	PTHR31707:SF53	SUBFAMILY NOT NAMED	coord: 1..185 score: 1.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G02460	Csa1G009900	Cucumber (Chinese Long) v2	cpicuB000
CSPI01G02460	MELO3C017187	Melon (DHL92) v3.5.1	cpimeB055
CSPI01G02460	ClCG06G016040	Watermelon (Charleston Gray)	cpiwcgB083
CSPI01G02460	Lsi06G014520	Bottle gourd (USVL1VR-Ls)	cpilsiB070
CSPI01G02460	MELO3C017187.2	Melon (DHL92) v3.6.1	cpimedB049
CSPI01G02460	CsaV3_1G002510	Cucumber (Chinese Long) v3	cpicucB001
CSPI01G02460	Cla97C06G125610	Watermelon (97103) v2	cpiwmbB079
CSPI01G02460	Bhi02G000209	Wax gourd	cpiwgoB098
CSPI01G02460	Cucsa.197850	Cucumber (Gy14) v1	cgycpiB310
CSPI01G02460	CmaCh11G001320	Cucurbita maxima (Rimu)	cmacpiB098
CSPI01G02460	CmoCh11G001800	Cucurbita moschata (Rifu)	cmocpiB079
CSPI01G02460	Cp4.1LG04g14920	Cucurbita pepo (Zucchini)	cpecpiB656
CSPI01G02460	Carg18201	Silver-seed gourd	carcpiB0645

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G02460	Wax gourd	cpiwgoB053
CSPI01G02460	Wax gourd	cpiwgoB071
CSPI01G02460	Wild cucumber (PI 183967)	cpicpiB000
CSPI01G02460	Wild cucumber (PI 183967)	cpicpiB029
CSPI01G02460	Cucumber (Gy14) v1	cgycpiB138
CSPI01G02460	Cucumber (Gy14) v1	cgycpiB427
CSPI01G02460	Cucurbita maxima (Rimu)	cmacpiB049
CSPI01G02460	Cucurbita maxima (Rimu)	cmacpiB432
CSPI01G02460	Cucurbita maxima (Rimu)	cmacpiB693
CSPI01G02460	Cucurbita moschata (Rifu)	cmocpiB040
CSPI01G02460	Cucurbita moschata (Rifu)	cmocpiB422
CSPI01G02460	Cucumber (Chinese Long) v2	cpicuB005
CSPI01G02460	Cucumber (Chinese Long) v2	cpicuB037
CSPI01G02460	Melon (DHL92) v3.5.1	cpimeB005
CSPI01G02460	Melon (DHL92) v3.5.1	cpimeB026
CSPI01G02460	Watermelon (Charleston Gray)	cpiwcgB060
CSPI01G02460	Watermelon (Charleston Gray)	cpiwcgB066
CSPI01G02460	Watermelon (97103) v1	cpiwmB039
CSPI01G02460	Watermelon (97103) v1	cpiwmB067
CSPI01G02460	Watermelon (97103) v1	cpiwmB099
CSPI01G02460	Cucurbita pepo (Zucchini)	cpecpiB361
CSPI01G02460	Cucurbita pepo (Zucchini)	cpecpiB538
CSPI01G02460	Bottle gourd (USVL1VR-Ls)	cpilsiB024
CSPI01G02460	Bottle gourd (USVL1VR-Ls)	cpilsiB055
CSPI01G02460	Melon (DHL92) v3.6.1	cpimedB005
CSPI01G02460	Melon (DHL92) v3.6.1	cpimedB028
CSPI01G02460	Cucumber (Gy14) v2	cgybcpiB001
CSPI01G02460	Cucumber (Gy14) v2	cgybcpiB004
CSPI01G02460	Silver-seed gourd	carcpiB0057
CSPI01G02460	Silver-seed gourd	carcpiB0499
CSPI01G02460	Cucumber (Chinese Long) v3	cpicucB003
CSPI01G02460	Cucumber (Chinese Long) v3	cpicucB044
CSPI01G02460	Watermelon (97103) v2	cpiwmbB056
CSPI01G02460	Watermelon (97103) v2	cpiwmbB063