Bhi10G001824 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi10G001824
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Rapid ALkalinization Factor
Location	chr10 : 57370209 .. 57371675 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGAATTGACATTGATATTTTAAACCTTGAAAGAGAAGAGAACGCGTAGGGAAGGAGTGAAGAGGTGGGCATTGATTAATAACAGCTGTACTCCAATCACACATTAACGACACTCTCTGCTCCATCTTCATGTGACTTAACTTCATCTCTCTCTCTCCCCCTTCTCTCTAAACCCCCCATCCCCTTCCTTCCTTCTCTCTCATTCTCAATTCCCAATTCCCAATGGCTTCCAAATTCCTCCTCCTCCTCTTCACTATCCTTCTCTTCATTTCTTCCTCTTCCGCCCATCTCTCCGTCGACACTTCCCTCAAGCTCATGGCCGACGCCCTCGAATGGCCCACCACCACTTCCCTCATCGAATCCGCCACCGCCACCGACATCGACGACGACGAACTCGACCTTGAACAAGACCCCCGCAGATCTCTCTTCTGGAGGCGAGTCCACTACTACATTTCTTACGGCGCCCTCGCCGCCAACCGGATCCCTTGCCCGCCCCGCTCCGGCCGCCCTTACTACACTCACAACTGCTACAAGGCTCGCGGCCCTGTCAATCCTTACTCCCGCGGCTGCTCCGCCATTACCCGTTGCCGCCGCTGATCTCTCTACACTTTAAACGCCGTCGTATTTCCACTTTCCCTAATTTGTTGATCTCATCTGTATTTTGTCGGTTTTTAGGGCTTGGAGTTGGAACAACAGAGACGACATGTAGTTTGGGGTTTTTTTTTTTTATTTAATTCTTATTTTTATAATACTTTTTTGATTGTTCCGCAATCTAGGGCTCGGAAAACGGCGTCGGTTTATTGGCTGAGGTATTATTATTTATTACTGCTATTTAATTATGATGATGATGTGTCCCTTGCTATCTGAACTGGATTATATAAATTCAATATTGTTGTTTATTAGTACTATTATTACGTAATTATATTTTAATCATATTTGATTATGGGTTGATGCTCGAATCACGCGGAAATGTCGGTGGACGGTGTTGTTTTTTTAATTCGTGGTAAATTATTTTTTTTACTGTGCGGCAGCAGTTAATTTCTCAGATTTTTTTTTTTTAAAAGGATAGACATTACTTCCACCCTAATTTTCTTCTTTTATTATCTATTTTTTTTACTAATAATTCTAAAAAATAAGCTAAATTTTAAAAACTAACAAAATGTTACTTTCAAAATTTTATTTTTGTTTTTAAAATTTAGCTAGAAATTTAATCATAATAAAGGTACAAATTATTGTAAAAAAATAGAAAATAGGTTTAATTTTCAAAAACAAAAAAATATAAAATAAAATGGTTATTAAAGAAGTCTCAATATATGTATATGGTGTTAGCAGAGCTGGAAATTAATAGATGGATGATGTGAACTCTGCTGTGGAATTTTGAGTGGGTTTACTTTGTTCATAACTTTTTTCTTAAAAGGGGCATATTCCTCTAACTTTTAACTTTTAAAGGTCTTCTTTAATTTAGT

mRNA sequence

GTTGAATTGACATTGATATTTTAAACCTTGAAAGAGAAGAGAACGCGTAGGGAAGGAGTGAAGAGGTGGGCATTGATTAATAACAGCTGTACTCCAATCACACATTAACGACACTCTCTGCTCCATCTTCATGTGACTTAACTTCATCTCTCTCTCTCCCCCTTCTCTCTAAACCCCCCATCCCCTTCCTTCCTTCTCTCTCATTCTCAATTCCCAATTCCCAATGGCTTCCAAATTCCTCCTCCTCCTCTTCACTATCCTTCTCTTCATTTCTTCCTCTTCCGCCCATCTCTCCGTCGACACTTCCCTCAAGCTCATGGCCGACGCCCTCGAATGGCCCACCACCACTTCCCTCATCGAATCCGCCACCGCCACCGACATCGACGACGACGAACTCGACCTTGAACAAGACCCCCGCAGATCTCTCTTCTGGAGGCGAGTCCACTACTACATTTCTTACGGCGCCCTCGCCGCCAACCGGATCCCTTGCCCGCCCCGCTCCGGCCGCCCTTACTACACTCACAACTGCTACAAGGCTCGCGGCCCTGTCAATCCTTACTCCCGCGGCTGCTCCGCCATTACCCGTTGCCGCCGCTGATCTCTCTACACTTTAAACGCCGTCGTATTTCCACTTTCCCTAATTTGTTGATCTCATCTGTATTTTGTCGGTTTTTAGGGCTTGGAGTTGGAACAACAGAGACGACATGTAGTTTGGGGTTTTTTTTTTTTATTTAATTCTTATTTTTATAATACTTTTTTGATTGTTCCGCAATCTAGGGCTCGGAAAACGGCGTCGGTTTATTGGCTGAGGTATTATTATTTATTACTGCTATTTAATTATGATGATGATGTGTCCCTTGCTATCTGAACTGGATTATATAAATTCAATATTGTTGTTTATTAGTACTATTATTACGTAATTATATTTTAATCATATTTGATTATGGGTTGATGCTCGAATCACGCGGAAATGTCGGTGGACGGTGTTGTTTTTTTAATTCGTGGTAAATTATTTTTTTTACTGTGCGGCAGCAGTTAATTTCTCAGATTTTTTTTTTTTAAAAGGATAGACATTACTTCCACCCTAATTTTCTTCTTTTATTATCTATTTTTTTTACTAATAATTCTAAAAAATAAGCTAAATTTTAAAAACTAACAAAATGTTACTTTCAAAATTTTATTTTTGTTTTTAAAATTTAGCTAGAAATTTAATCATAATAAAGGTACAAATTATTGTAAAAAAATAGAAAATAGGTTTAATTTTCAAAAACAAAAAAATATAAAATAAAATGGTTATTAAAGAAGTCTCAATATATGTATATGGTGTTAGCAGAGCTGGAAATTAATAGATGGATGATGTGAACTCTGCTGTGGAATTTTGAGTGGGTTTACTTTGTTCATAACTTTTTTCTTAAAAGGGGCATATTCCTCTAACTTTTAACTTTTAAAGGTCTTCTTTAATTTAGT

Coding sequence (CDS)

ATGGCTTCCAAATTCCTCCTCCTCCTCTTCACTATCCTTCTCTTCATTTCTTCCTCTTCCGCCCATCTCTCCGTCGACACTTCCCTCAAGCTCATGGCCGACGCCCTCGAATGGCCCACCACCACTTCCCTCATCGAATCCGCCACCGCCACCGACATCGACGACGACGAACTCGACCTTGAACAAGACCCCCGCAGATCTCTCTTCTGGAGGCGAGTCCACTACTACATTTCTTACGGCGCCCTCGCCGCCAACCGGATCCCTTGCCCGCCCCGCTCCGGCCGCCCTTACTACACTCACAACTGCTACAAGGCTCGCGGCCCTGTCAATCCTTACTCCCGCGGCTGCTCCGCCATTACCCGTTGCCGCCGCTGA

Protein sequence

MASKFLLLLFTILLFISSSSAHLSVDTSLKLMADALEWPTTTSLIESATATDIDDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSAITRCRR

Homology

[Close]

BLAST of Bhi10G001824 vs. TAIR10
Match: AT5G67070.1 (ralf-like 34)

HSP 1 Score: 134.4 bits (337), Expect = 4.7e-32
Identity = 74/127 (58.27%), Postives = 90/127 (70.87%), Query Frame = 0


Query: 6   LLLLFTILLFIS-SSSAHLSVDTSLKLMADALEWPTTTS----LI---ESATATDIDDDE 65
           LLL+ ++L FIS   S  LS + SL L+ D  +WP + S    +I   ES   T+ DD  
Sbjct: 8   LLLILSLLTFISLQRSESLSDNPSLTLLPDGFDWPISHSDEFDIIDGEESFEVTEEDDGV 67

Query: 66  LDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCS 125
            D     RRSL+WRR  YYISYGAL+ANR+PCPPRSGR YYTHNC++ARGPV+PYSRGCS
Sbjct: 68  TD-----RRSLYWRRTKYYISYGALSANRVPCPPRSGRSYYTHNCFRARGPVHPYSRGCS 127

[Close]

BLAST of Bhi10G001824 vs. TAIR10
Match: AT3G16570.1 (rapid alkalinization factor 23)

HSP 1 Score: 61.6 bits (148), Expect = 3.9e-10
Identity = 40/71 (56.34%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 54  DDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYS 113
           ++ E+D E + RR L  RR   YISYGAL  N IPC  R G  YY  NC +     NPYS
Sbjct: 75  EEFEMDSEIN-RRILATRR---YISYGALRRNTIPC-SRRGASYY--NCRRG-AQANPYS 134

Query: 114 RGCSAITRCRR 125
           RGCSAITRCRR
Sbjct: 135 RGCSAITRCRR 137

[Close]

BLAST of Bhi10G001824 vs. TAIR10
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 60.8 bits (146), Expect = 6.6e-10
Identity = 32/71 (45.07%), Postives = 44/71 (61.97%), Query Frame = 0


Query: 54  DDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYS 113
           +++E++ + D  R +  ++   YISYGA+  N +PC  R G  YY  NC +     NPYS
Sbjct: 55  EEEEMEFDSDISRRILAQK--KYISYGAMRRNSVPC-SRRGASYY--NCQRG-AQANPYS 114

Query: 114 RGCSAITRCRR 125
           RGCS ITRCRR
Sbjct: 115 RGCSTITRCRR 119

[Close]

BLAST of Bhi10G001824 vs. TAIR10
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 60.1 bits (144), Expect = 1.1e-09
Identity = 39/82 (47.56%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 43  SLIESATATDIDDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNC 102
           ++ E + +T  ++ E+D E + RR L   +   YISYGAL  N +PC  R G  YY  NC
Sbjct: 43  TIAECSLSTAEEEFEMDSEIN-RRILATTK---YISYGALRRNTVPC-SRRGASYY--NC 102

Query: 103 YKARGPVNPYSRGCSAITRCRR 125
            +     NPYSRGCSAITRCRR
Sbjct: 103 RRG-AQANPYSRGCSAITRCRR 116

[Close]

BLAST of Bhi10G001824 vs. TAIR10
Match: AT2G33775.1 (ralf-like 19)

HSP 1 Score: 56.2 bits (134), Expect = 1.6e-08
Identity = 50/127 (39.37%), Postives = 60/127 (47.24%), Query Frame = 0


Query: 1   MASKFLLLLFTILLFISSSSAHLSVDTSLKLMADALEWPTTTSLIESATATDIDDDELDL 60
           M  K LL+L  + L + + SA+ +             W  T S +        +D ELD 
Sbjct: 1   MGIKILLILGLLTLAVVAESANAT-------------WTLTKSCVNGQGCIG-EDGELDY 60

Query: 61  EQDP---RRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCS 120
             D    RR L  RR   YISYGAL  N +PC  R GR YY  +C K R   NPY RGCS
Sbjct: 61  LMDSETNRRQLAARR--SYISYGALRKNNVPC-SRRGRSYY--DC-KKRKRANPYRRGCS 107

Query: 121 AITRCRR 125
            IT C R
Sbjct: 121 VITHCYR 107

[Close]

BLAST of Bhi10G001824 vs. Swiss-Prot
Match: sp|Q9FHA6|RLF34_ARATH (Protein RALF-like 34 OS=Arabidopsis thaliana OX=3702 GN=RALFL34 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 8.5e-31
Identity = 74/127 (58.27%), Postives = 90/127 (70.87%), Query Frame = 0


Query: 6   LLLLFTILLFIS-SSSAHLSVDTSLKLMADALEWPTTTS----LI---ESATATDIDDDE 65
           LLL+ ++L FIS   S  LS + SL L+ D  +WP + S    +I   ES   T+ DD  
Sbjct: 8   LLLILSLLTFISLQRSESLSDNPSLTLLPDGFDWPISHSDEFDIIDGEESFEVTEEDDGV 67

Query: 66  LDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCS 125
            D     RRSL+WRR  YYISYGAL+ANR+PCPPRSGR YYTHNC++ARGPV+PYSRGCS
Sbjct: 68  TD-----RRSLYWRRTKYYISYGALSANRVPCPPRSGRSYYTHNCFRARGPVHPYSRGCS 127

[Close]

BLAST of Bhi10G001824 vs. Swiss-Prot
Match: sp|Q9LUS7|RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 7.0e-09
Identity = 40/71 (56.34%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 54  DDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYS 113
           ++ E+D E + RR L  RR   YISYGAL  N IPC  R G  YY  NC +     NPYS
Sbjct: 75  EEFEMDSEIN-RRILATRR---YISYGALRRNTIPC-SRRGASYY--NCRRG-AQANPYS 134

Query: 114 RGCSAITRCRR 125
           RGCSAITRCRR
Sbjct: 135 RGCSAITRCRR 137

[Close]

BLAST of Bhi10G001824 vs. Swiss-Prot
Match: sp|Q945T0|RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 9.2e-09
Identity = 34/70 (48.57%), Postives = 42/70 (60.00%), Query Frame = 0


Query: 54  DDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYS 113
           +++E +L+ +  R +       YISYGAL  N +PC  R G  YY  NC K     NPYS
Sbjct: 51  EEEEFELDSESNRRIL--ATKKYISYGALQKNSVPC-SRRGASYY--NC-KPGAQANPYS 110

Query: 114 RGCSAITRCR 124
           RGCSAITRCR
Sbjct: 111 RGCSAITRCR 114

[Close]

BLAST of Bhi10G001824 vs. Swiss-Prot
Match: sp|Q9MA62|RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.2e-08
Identity = 32/71 (45.07%), Postives = 44/71 (61.97%), Query Frame = 0


Query: 54  DDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYS 113
           +++E++ + D  R +  ++   YISYGA+  N +PC  R G  YY  NC +     NPYS
Sbjct: 55  EEEEMEFDSDISRRILAQK--KYISYGAMRRNSVPC-SRRGASYY--NCQRG-AQANPYS 114

Query: 114 RGCSAITRCRR 125
           RGCS ITRCRR
Sbjct: 115 RGCSTITRCRR 119

[Close]

BLAST of Bhi10G001824 vs. Swiss-Prot
Match: sp|Q8L9P8|RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-08
Identity = 39/82 (47.56%), Postives = 50/82 (60.98%), Query Frame = 0


Query: 43  SLIESATATDIDDDELDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNC 102
           ++ E + +T  ++ E+D E + RR L   +   YISYGAL  N +PC  R G  YY  NC
Sbjct: 43  TIAECSLSTAEEEFEMDSEIN-RRILATTK---YISYGALRRNTVPC-SRRGASYY--NC 102

Query: 103 YKARGPVNPYSRGCSAITRCRR 125
            +     NPYSRGCSAITRCRR
Sbjct: 103 RRG-AQANPYSRGCSAITRCRR 116

[Close]

BLAST of Bhi10G001824 vs. TrEMBL
Match: tr|A0A1S3C7S7|A0A1S3C7S7_CUCME (protein RALF-like 34 OS=Cucumis melo OX=3656 GN=LOC103497450 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 6.9e-54
Identity = 113/126 (89.68%), Postives = 119/126 (94.44%), Query Frame = 0


Query: 1   MASKFLL-LLFT-ILLFISSSSAHLSVDTSLKLMADALEWPTTTSLIESATATDIDDDEL 60
           MASK LL LLFT +LLF+SSSSAHLSVDTSLKLMADALEWPTTTSLI+S T  D+DDD L
Sbjct: 1   MASKSLLFLLFTLLLLFLSSSSAHLSVDTSLKLMADALEWPTTTSLIQSPTDDDLDDD-L 60

Query: 61  DLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA 120
           DL+QDPRRSLFWRRVHYYISYGAL+ANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA
Sbjct: 61  DLQQDPRRSLFWRRVHYYISYGALSANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA 120

Query: 121 ITRCRR 125
           ITRCRR
Sbjct: 121 ITRCRR 125

[Close]

BLAST of Bhi10G001824 vs. TrEMBL
Match: tr|A0A0A0LMS2|A0A0A0LMS2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292820 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.4e-53
Identity = 111/126 (88.10%), Postives = 118/126 (93.65%), Query Frame = 0


Query: 1   MASKFLLL-LFT-ILLFISSSSAHLSVDTSLKLMADALEWPTTTSLIESATATDIDDDEL 60
           MASK LL  LFT +LLF+SSSSAHLSVDTSLKLMADALEWPTTTSLI+S T  D+DDD L
Sbjct: 1   MASKSLLFPLFTLLLLFLSSSSAHLSVDTSLKLMADALEWPTTTSLIQSPTEDDLDDD-L 60

Query: 61  DLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA 120
           DL+QDPRRSLFWRRVHYYISYGAL+ANRIPCPPRSGRPYYTHNCYKARGPVNPY+RGCSA
Sbjct: 61  DLQQDPRRSLFWRRVHYYISYGALSANRIPCPPRSGRPYYTHNCYKARGPVNPYTRGCSA 120

Query: 121 ITRCRR 125
           ITRCRR
Sbjct: 121 ITRCRR 125

[Close]

BLAST of Bhi10G001824 vs. TrEMBL
Match: tr|A0A2H3XI67|A0A2H3XI67_PHODC (protein RALF-like 34 OS=Phoenix dactylifera OX=42345 GN=LOC103703194 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 9.4e-35
Identity = 78/129 (60.47%), Postives = 100/129 (77.52%), Query Frame = 0


Query: 7   LLLFTILLFISSSSAHLSV------DTSLKLMADALEWPTTTSLIESATA----TDIDDD 66
           L  F++LLF+S S+ +++V      DTSL+LM DA+EWPTTTSL  S+++     ++D++
Sbjct: 6   LFSFSLLLFLSFSTYNVAVVEAQADDTSLRLMTDAMEWPTTTSLYSSSSSYPFGHEVDEE 65

Query: 67  EL-DLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRG 125
           EL +     RR+LFW R+ YYISYGAL+ANR+PCPPRSGR YYTHNCY+ARGPV PY RG
Sbjct: 66  ELEEAANRNRRALFWYRIRYYISYGALSANRVPCPPRSGRSYYTHNCYRARGPVRPYHRG 125

[Close]

BLAST of Bhi10G001824 vs. TrEMBL
Match: tr|I1LHE0|I1LHE0_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100305899 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.7e-34
Identity = 80/124 (64.52%), Postives = 94/124 (75.81%), Query Frame = 0


Query: 7   LLLFTILLFISSSSAHLSV-----DTSLKLMADALEWPTTTSLIESATATDIDDDELDLE 66
           LL   +LLF  + ++H++V     DT+  LM+DALEWPTT SL       D DD + D+E
Sbjct: 4   LLTLALLLFCIAFTSHVAVQAHMEDTAFNLMSDALEWPTTMSLY------DEDDAQEDVE 63

Query: 67  QD-PRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSAIT 125
               RRSLFWRR+ YYISYGAL+ANRIPCPPRSGR YYTHNCY+ARGPV+PYSRGCSAIT
Sbjct: 64  NAYSRRSLFWRRMKYYISYGALSANRIPCPPRSGRSYYTHNCYRARGPVHPYSRGCSAIT 121

[Close]

BLAST of Bhi10G001824 vs. TrEMBL
Match: tr|A0A1S3VP27|A0A1S3VP27_VIGRR (protein RALF-like 34 OS=Vigna radiata var. radiata OX=3916 GN=LOC106777124 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.0e-33
Identity = 84/130 (64.62%), Postives = 94/130 (72.31%), Query Frame = 0


Query: 1   MASKFLLL---LFTILLFISSSSAHLSV-DTSLKLMADALEWPTTTSLIESATATDID-D 60
           MAS   LL   LF   LF      H  + DT L LM+DAL+WPTT SL +     D+D D
Sbjct: 1   MASSLPLLTLALFLCFLFTHHGGVHAQIDDTGLNLMSDALDWPTTMSLYD-----DLDED 60

Query: 61  DELDLEQD-PRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSR 120
           +E D+E    RRSLFWRR+ YYISYGAL+ANRIPCPPRSGR YYTHNCYKARGPV+PYSR
Sbjct: 61  EEEDVESGFSRRSLFWRRMKYYISYGALSANRIPCPPRSGRSYYTHNCYKARGPVHPYSR 120

Query: 121 GCSAITRCRR 125
           GCS ITRCRR
Sbjct: 121 GCSVITRCRR 125

[Close]

BLAST of Bhi10G001824 vs. NCBI nr
Match: XP_008457868.1 (PREDICTED: protein RALF-like 34 [Cucumis melo])

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-53
Identity = 113/126 (89.68%), Postives = 119/126 (94.44%), Query Frame = 0


Query: 1   MASKFLL-LLFT-ILLFISSSSAHLSVDTSLKLMADALEWPTTTSLIESATATDIDDDEL 60
           MASK LL LLFT +LLF+SSSSAHLSVDTSLKLMADALEWPTTTSLI+S T  D+DDD L
Sbjct: 1   MASKSLLFLLFTLLLLFLSSSSAHLSVDTSLKLMADALEWPTTTSLIQSPTDDDLDDD-L 60

Query: 61  DLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA 120
           DL+QDPRRSLFWRRVHYYISYGAL+ANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA
Sbjct: 61  DLQQDPRRSLFWRRVHYYISYGALSANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA 120

Query: 121 ITRCRR 125
           ITRCRR
Sbjct: 121 ITRCRR 125

[Close]

BLAST of Bhi10G001824 vs. NCBI nr
Match: XP_004148071.1 (PREDICTED: protein RALF-like 34 [Cucumis sativus] >KGN62052.1 hypothetical protein Csa_2G292820 [Cucumis sativus])

HSP 1 Score: 216.5 bits (550), Expect = 5.2e-53
Identity = 111/126 (88.10%), Postives = 118/126 (93.65%), Query Frame = 0


Query: 1   MASKFLLL-LFT-ILLFISSSSAHLSVDTSLKLMADALEWPTTTSLIESATATDIDDDEL 60
           MASK LL  LFT +LLF+SSSSAHLSVDTSLKLMADALEWPTTTSLI+S T  D+DDD L
Sbjct: 1   MASKSLLFPLFTLLLLFLSSSSAHLSVDTSLKLMADALEWPTTTSLIQSPTEDDLDDD-L 60

Query: 61  DLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSA 120
           DL+QDPRRSLFWRRVHYYISYGAL+ANRIPCPPRSGRPYYTHNCYKARGPVNPY+RGCSA
Sbjct: 61  DLQQDPRRSLFWRRVHYYISYGALSANRIPCPPRSGRPYYTHNCYKARGPVNPYTRGCSA 120

Query: 121 ITRCRR 125
           ITRCRR
Sbjct: 121 ITRCRR 125

[Close]

BLAST of Bhi10G001824 vs. NCBI nr
Match: XP_023513191.1 (protein RALF-like 34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 184.5 bits (467), Expect = 2.2e-43
Identity = 96/125 (76.80%), Postives = 101/125 (80.80%), Query Frame = 0


Query: 1   MASKFLLLLFTI-LLFISSSSAHLSVDTSLKLMADALEWPTTTSLIESATATDIDDDELD 60
           MASK L   FTI LL +S++SAHL VDTSLKLMADALEWPTT S+ +             
Sbjct: 1   MASKLLFPFFTILLLLLSAASAHLPVDTSLKLMADALEWPTTISIPD----XXXXXXXXX 60

Query: 61  LEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSAI 120
           L QDPRRSLFW RVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSAI
Sbjct: 61  LHQDPRRSLFWSRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCSAI 120

Query: 121 TRCRR 125
           TRCRR
Sbjct: 121 TRCRR 121

[Close]

BLAST of Bhi10G001824 vs. NCBI nr
Match: XP_022928024.1 (protein RALF-like 34 [Cucurbita moschata])

HSP 1 Score: 183.3 bits (464), Expect = 4.9e-43
Identity = 96/127 (75.59%), Postives = 100/127 (78.74%), Query Frame = 0


Query: 1   MASKFLLLLFTI-LLFISSSSAHLSVDTSLKLMADALEWPTTTSL--IESATATDIDDDE 60
           MASK L   FTI LL +S++SAHL VDTSLKLMADALEWPTT S+               
Sbjct: 1   MASKLLFPFFTILLLLLSAASAHLPVDTSLKLMADALEWPTTISIPXXXXXXXXXXXXXX 60

Query: 61  LDLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCS 120
             L QDPRRSLFW RVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCS
Sbjct: 61  XXLHQDPRRSLFWSRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRGCS 120

Query: 121 AITRCRR 125
           AITRCRR
Sbjct: 121 AITRCRR 127

[Close]

BLAST of Bhi10G001824 vs. NCBI nr
Match: XP_008784189.1 (PREDICTED: protein RALF-like 34 [Phoenix dactylifera])

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-34
Identity = 78/129 (60.47%), Postives = 100/129 (77.52%), Query Frame = 0


Query: 7   LLLFTILLFISSSSAHLSV------DTSLKLMADALEWPTTTSLIESATA----TDIDDD 66
           L  F++LLF+S S+ +++V      DTSL+LM DA+EWPTTTSL  S+++     ++D++
Sbjct: 6   LFSFSLLLFLSFSTYNVAVVEAQADDTSLRLMTDAMEWPTTTSLYSSSSSYPFGHEVDEE 65

Query: 67  EL-DLEQDPRRSLFWRRVHYYISYGALAANRIPCPPRSGRPYYTHNCYKARGPVNPYSRG 125
           EL +     RR+LFW R+ YYISYGAL+ANR+PCPPRSGR YYTHNCY+ARGPV PY RG
Sbjct: 66  ELEEAANRNRRALFWYRIRYYISYGALSANRVPCPPRSGRSYYTHNCYRARGPVRPYHRG 125

The following BLAST results are available for this feature:

BLAST of Bhi10G001824 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G67070.1	4.7e-32	58.27	ralf-like 34	[more]
AT3G16570.1	3.9e-10	56.34	rapid alkalinization factor 23	[more]
AT3G05490.1	6.6e-10	45.07	ralf-like 22	[more]
AT4G15800.1	1.1e-09	47.56	ralf-like 33	[more]
AT2G33775.1	1.6e-08	39.37	ralf-like 19	[more]

BLAST of Bhi10G001824 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FHA6\|RLF34_ARATH	8.5e-31	58.27	Protein RALF-like 34 OS=Arabidopsis thaliana OX=3702 GN=RALFL34 PE=2 SV=1	[more]
sp\|Q9LUS7\|RLF23_ARATH	7.0e-09	56.34	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]
sp\|Q945T0\|RALF_TOBAC	9.2e-09	48.57	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
sp\|Q9MA62\|RLF22_ARATH	1.2e-08	45.07	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
sp\|Q8L9P8\|RLF33_ARATH	2.0e-08	47.56	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]

BLAST of Bhi10G001824 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C7S7\|A0A1S3C7S7_CUCME	6.9e-54	89.68	protein RALF-like 34 OS=Cucumis melo OX=3656 GN=LOC103497450 PE=4 SV=1	[more]
tr\|A0A0A0LMS2\|A0A0A0LMS2_CUCSA	3.4e-53	88.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292820 PE=4 SV=1	[more]
tr\|A0A2H3XI67\|A0A2H3XI67_PHODC	9.4e-35	60.47	protein RALF-like 34 OS=Phoenix dactylifera OX=42345 GN=LOC103703194 PE=4 SV=1	[more]
tr\|I1LHE0\|I1LHE0_SOYBN	4.7e-34	64.52	Uncharacterized protein OS=Glycine max OX=3847 GN=100305899 PE=4 SV=1	[more]
tr\|A0A1S3VP27\|A0A1S3VP27_VIGRR	1.0e-33	64.62	protein RALF-like 34 OS=Vigna radiata var. radiata OX=3916 GN=LOC106777124 PE=4 ...	[more]

BLAST of Bhi10G001824 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008457868.1	1.1e-53	89.68	PREDICTED: protein RALF-like 34 [Cucumis melo]	[more]
XP_004148071.1	5.2e-53	88.10	PREDICTED: protein RALF-like 34 [Cucumis sativus] >KGN62052.1 hypothetical prote...	[more]
XP_023513191.1	2.2e-43	76.80	protein RALF-like 34 [Cucurbita pepo subsp. pepo]	[more]
XP_022928024.1	4.9e-43	75.59	protein RALF-like 34 [Cucurbita moschata]	[more]
XP_008784189.1	1.4e-34	60.47	PREDICTED: protein RALF-like 34 [Phoenix dactylifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
biological_process	GO:0006949	syncytium formation
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
biological_process	GO:0008150	biological_process
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M001824	Bhi10M001824	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 65..124 e-value: 8.0E-21 score: 74.6
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 5..124
None	No IPR available	PANTHER	PTHR33136:SF7	PROTEIN RALF-LIKE 26-RELATED	coord: 5..124

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi10G001824	Csa2G292820	Cucumber (Chinese Long) v2	cuwgoB175
Bhi10G001824	MELO3C020932	Melon (DHL92) v3.5.1	mewgoB129
Bhi10G001824	CSPI02G14730	Wild cucumber (PI 183967)	cpiwgoB172
Bhi10G001824	Cucsa.366420	Cucumber (Gy14) v1	cgywgoB741
Bhi10G001824	CmaCh20G011300	Cucurbita maxima (Rimu)	cmawgoB0694
Bhi10G001824	CmoCh20G011640	Cucurbita moschata (Rifu)	cmowgoB0689
Bhi10G001824	Lsi10G006340	Bottle gourd (USVL1VR-Ls)	lsiwgoB072
Bhi10G001824	Cp4.1LG16g00300	Cucurbita pepo (Zucchini)	cpewgoB0356
Bhi10G001824	CsGy2G014760	Cucumber (Gy14) v2	cgybwgoB169
Bhi10G001824	MELO3C020932.2	Melon (DHL92) v3.6.1	medwgoB132
Bhi10G001824	Carg20939	Silver-seed gourd	carwgoB1163
Bhi10G001824	CsaV3_2G017290	Cucumber (Chinese Long) v3	cucwgoB181
Bhi10G001824	Cla020140	Watermelon (97103) v1	wgowmB380
Bhi10G001824	Cla97C02G038920	Watermelon (97103) v2	wgowmbB354

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi10G001824	Cucumber (Gy14) v2	cgybwgoB174
Bhi10G001824	Cucurbita maxima (Rimu)	cmawgoB0750
Bhi10G001824	Cucurbita moschata (Rifu)	cmowgoB0741
Bhi10G001824	Cucurbita pepo (Zucchini)	cpewgoB0962
Bhi10G001824	Watermelon (Charleston Gray)	wcgwgoB284
Bhi10G001824	Watermelon (Charleston Gray)	wcgwgoB296
Bhi10G001824	Watermelon (97103) v2	wgowmbB366
Bhi10G001824	Watermelon (97103) v1	wgowmB394
Bhi10G001824	Silver-seed gourd	carwgoB1242