Bhi05G000788 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi05G000788
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cysteine proteinase inhibitor
Location	chr5 : 33545934 .. 33546542 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACAACTCACGAAAACCAGACAAACCACACCCCAAACTTTGTGCCTAAAATTAATTAGGTCATCAAAATGAGTTCAACATTTCCTTGTGGTTGTTCCAAAAAGTGGATTAAAATCACTGATCTTGAGACTCCTTGTGTTGAAGACAATGCAAAATTTGCCATAGAAGAGTTCAACATTAAACATAATGGGAGCTTGAGATACAAGAAGGTTATTGAAGGTTGGTGTAAGGAGTTGGATGAAAGCCACATAAGGTATCGTCTCCTTGTTCAAGCGAACGATTGTCTTAGACGTCTACTCGAATTTGAGGCTATTGTGTTCGCACACTATTCTGAAAATGGAGAGACTACTAGGATTCTTGAATCTTTTGAGCAACGTTTCTAAAATAAGTCATCAAACATCCTTTTGTGTGTTCTAGGTTACAACCTTGGTCGCCTATATTGGATGTGTTTTTGTCTTTTCACTATCTATATATTATATTTGAATAATCATGTAATGAGTATAAGATTTAATATATATATATATACCTACCGTTGTATTGCATCATTTTCTCATCGAAACGAATGATAGTTATGTTGTGGGATGATCACTATCCTAAAAGAAATTTGAC

mRNA sequence

AAACAACTCACGAAAACCAGACAAACCACACCCCAAACTTTGTGCCTAAAATTAATTAGGTCATCAAAATGAGTTCAACATTTCCTTGTGGTTGTTCCAAAAAGTGGATTAAAATCACTGATCTTGAGACTCCTTGTGTTGAAGACAATGCAAAATTTGCCATAGAAGAGTTCAACATTAAACATAATGGGAGCTTGAGATACAAGAAGGTTATTGAAGGTTGGTGTAAGGAGTTGGATGAAAGCCACATAAGGTATCGTCTCCTTGTTCAAGCGAACGATTGTCTTAGACGTCTACTCGAATTTGAGGCTATTGTGTTCGCACACTATTCTGAAAATGGAGAGACTACTAGGATTCTTGAATCTTTTGAGCAACGTTTCTAAAATAAGTCATCAAACATCCTTTTGTGTGTTCTAGGTTACAACCTTGGTCGCCTATATTGGATGTGTTTTTGTCTTTTCACTATCTATATATTATATTTGAATAATCATGTAATGAGTATAAGATTTAATATATATATATATACCTACCGTTGTATTGCATCATTTTCTCATCGAAACGAATGATAGTTATGTTGTGGGATGATCACTATCCTAAAAGAAATTTGAC

Coding sequence (CDS)

ATGAGTTCAACATTTCCTTGTGGTTGTTCCAAAAAGTGGATTAAAATCACTGATCTTGAGACTCCTTGTGTTGAAGACAATGCAAAATTTGCCATAGAAGAGTTCAACATTAAACATAATGGGAGCTTGAGATACAAGAAGGTTATTGAAGGTTGGTGTAAGGAGTTGGATGAAAGCCACATAAGGTATCGTCTCCTTGTTCAAGCGAACGATTGTCTTAGACGTCTACTCGAATTTGAGGCTATTGTGTTCGCACACTATTCTGAAAATGGAGAGACTACTAGGATTCTTGAATCTTTTGAGCAACGTTTCTAA

Protein sequence

MSSTFPCGCSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDESHIRYRLLVQANDCLRRLLEFEAIVFAHYSENGETTRILESFEQRF

Homology

[Close]

BLAST of Bhi05G000788 vs. TrEMBL
Match: tr|E5GCR7|E5GCR7_CUCME (Phloem filament protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-20
Identity = 54/103 (52.43%), Postives = 75/103 (72.82%), Query Frame = 0


Query: 3   STFPCGCSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDE--SH 62
           S+ P GCSKKWIK+  +  PC++  A+  +EEF  K++ SL YK V+EGW +ELD+  + 
Sbjct: 2   SSLPSGCSKKWIKLPSVLIPCLQAIAEHGVEEFKKKYDVSLIYKNVVEGWYQELDDHGNT 61

Query: 63  IRYRLLVQANDCLRRLLEFEAIVFAHYSENGETTRI-LESFEQ 103
           IRYRL VQA DCLRRLL+FEAI+   +++N E + I LESF++
Sbjct: 62  IRYRLHVQAYDCLRRLLKFEAILLQQHAQNNEESTITLESFDR 104

[Close]

BLAST of Bhi05G000788 vs. TrEMBL
Match: tr|A0A0A0K471|A0A0A0K471_CUCSA (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G067340 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-20
Identity = 54/103 (52.43%), Postives = 73/103 (70.87%), Query Frame = 0


Query: 3   STFPCGCSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDE--SH 62
           S+ P  CSK+WIK  ++  PC++  A+  IEEF  KH  SL YK V+EGW KELD+  + 
Sbjct: 2   SSLPSICSKRWIKFPNVLIPCLQAIAEHGIEEFKKKHEVSLIYKNVVEGWYKELDDHGNT 61

Query: 63  IRYRLLVQANDCLRRLLEFEAIVFAHYSENGETTRI-LESFEQ 103
           IRYRL +QA DC+RRLL+FEA++   +++N E + I LESFEQ
Sbjct: 62  IRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQ 104

[Close]

BLAST of Bhi05G000788 vs. TrEMBL
Match: tr|P94012|P94012_CUCMA (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=PP1 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.5e-12
Identity = 35/74 (47.30%), Postives = 55/74 (74.32%), Query Frame = 0


Query: 11 KKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDESHIRYRLLVQAN 70
          +KWIKI D+   CV++ AK A+E+FN++H  SL+Y  + EGW  EL +++++YRL ++A 
Sbjct: 15 EKWIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHIRAI 74

Query: 71 DCLRRLLEFEAIVF 85
          D L+R L +EA+VF
Sbjct: 75 DFLQRSLLYEALVF 88

[Close]

BLAST of Bhi05G000788 vs. TrEMBL
Match: tr|Q7DMD5|Q7DMD5_CUCMA (Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=cPC7 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-10
Identity = 32/91 (35.16%), Postives = 63/91 (69.23%), Query Frame = 0


Query: 11  KKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDESHIRYRLLVQAN 70
           +KWIKI++L+   +++ +KFA+++FN+K   SLR+  + +GW  E+ + +I++R+ ++A 
Sbjct: 325 EKWIKISNLQFAFLQEVSKFALDDFNVKSGDSLRFDGIYDGWYMEMGQDNIKFRIHLRAK 384

Query: 71  DCLRRLLEFEAIVFAHYSENGETTRILESFE 102
           DCL R+  +EA V+     + +  +I+ESF+
Sbjct: 385 DCLSRVHHYEAYVYVKQFLS-KRIKIVESFK 414

[Close]

BLAST of Bhi05G000788 vs. TrEMBL
Match: tr|E5GCR6|E5GCR6_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.2e-08
Identity = 36/95 (37.89%), Postives = 57/95 (60.00%), Query Frame = 0


Query: 10  SKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDESHIRYRLLVQA 69
           SKKWI++ +++ P V + AKFA++ FN K+  +L ++ V E W  E++     Y + + A
Sbjct: 41  SKKWIQLHNVKAPDVLEAAKFAVDAFNKKYENNLEFQYVTEAWVHEVNNEK-EYSIELVA 100

Query: 70  NDCLRRLLEFEAIVFAHYSENGETTRILESFEQRF 105
            DC  RLLEF A+V     ++ +   IL SFE+ F
Sbjct: 101 KDCFHRLLEFHAVVIEKTVDH-KKIWILVSFERIF 133

[Close]

BLAST of Bhi05G000788 vs. NCBI nr
Match: ADN34265.1 (phloem filament protein [Cucumis melo subsp. melo])

HSP 1 Score: 108.2 bits (269), Expect = 1.7e-20
Identity = 54/103 (52.43%), Postives = 75/103 (72.82%), Query Frame = 0


Query: 3   STFPCGCSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDE--SH 62
           S+ P GCSKKWIK+  +  PC++  A+  +EEF  K++ SL YK V+EGW +ELD+  + 
Sbjct: 2   SSLPSGCSKKWIKLPSVLIPCLQAIAEHGVEEFKKKYDVSLIYKNVVEGWYQELDDHGNT 61

Query: 63  IRYRLLVQANDCLRRLLEFEAIVFAHYSENGETTRI-LESFEQ 103
           IRYRL VQA DCLRRLL+FEAI+   +++N E + I LESF++
Sbjct: 62  IRYRLHVQAYDCLRRLLKFEAILLQQHAQNNEESTITLESFDR 104

[Close]

BLAST of Bhi05G000788 vs. NCBI nr
Match: KGN43779.1 (Phloem filament protein [Cucumis sativus])

HSP 1 Score: 107.8 bits (268), Expect = 2.2e-20
Identity = 54/103 (52.43%), Postives = 73/103 (70.87%), Query Frame = 0


Query: 3   STFPCGCSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDE--SH 62
           S+ P  CSK+WIK  ++  PC++  A+  IEEF  KH  SL YK V+EGW KELD+  + 
Sbjct: 2   SSLPSICSKRWIKFPNVLIPCLQAIAEHGIEEFKKKHEVSLIYKNVVEGWYKELDDHGNT 61

Query: 63  IRYRLLVQANDCLRRLLEFEAIVFAHYSENGETTRI-LESFEQ 103
           IRYRL +QA DC+RRLL+FEA++   +++N E + I LESFEQ
Sbjct: 62  IRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQ 104

[Close]

BLAST of Bhi05G000788 vs. NCBI nr
Match: XP_022927698.1 (uncharacterized protein LOC111434515 [Cucurbita moschata])

HSP 1 Score: 83.2 bits (204), Expect = 5.8e-13
Identity = 35/74 (47.30%), Postives = 57/74 (77.03%), Query Frame = 0


Query: 11 KKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDESHIRYRLLVQAN 70
          +KWIKI D+ + CV++ AK A+E+FN++H  SL+Y+ + EGW  EL +++++YRL ++A 
Sbjct: 15 EKWIKIPDVSSHCVQEVAKLAVEQFNVQHGHSLKYESIHEGWFCELGQNNLKYRLHIRAI 74

Query: 71 DCLRRLLEFEAIVF 85
          D L+R L +EA+VF
Sbjct: 75 DFLQRSLLYEALVF 88

[Close]

BLAST of Bhi05G000788 vs. NCBI nr
Match: XP_022927869.1 (uncharacterized protein LOC111434636 isoform X1 [Cucurbita moschata])

HSP 1 Score: 82.4 bits (202), Expect = 9.9e-13
Identity = 37/81 (45.68%), Postives = 58/81 (71.60%), Query Frame = 0


Query: 7  CG---CSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDESHIRY 66
          CG     +KWIKI D+   CV++ AK A+E+FN++H  SL+Y+ + EGW  EL +++++Y
Sbjct: 8  CGQVPAEEKWIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYESIHEGWYCELGQNNLKY 67

Query: 67 RLLVQANDCLRRLLEFEAIVF 85
          RL ++A D L+R L +EA+VF
Sbjct: 68 RLHIRAIDFLQRSLLYEALVF 88

[Close]

BLAST of Bhi05G000788 vs. NCBI nr
Match: XP_022927870.1 (uncharacterized protein LOC111434636 isoform X2 [Cucurbita moschata])

HSP 1 Score: 82.4 bits (202), Expect = 9.9e-13
Identity = 37/81 (45.68%), Postives = 58/81 (71.60%), Query Frame = 0


Query: 7  CG---CSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDESHIRY 66
          CG     +KWIKI D+   CV++ AK A+E+FN++H  SL+Y+ + EGW  EL +++++Y
Sbjct: 8  CGQVPAEEKWIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYESIHEGWYCELGQNNLKY 67

Query: 67 RLLVQANDCLRRLLEFEAIVF 85
          RL ++A D L+R L +EA+VF
Sbjct: 68 RLHIRAIDFLQRSLLYEALVF 88

The following BLAST results are available for this feature:

BLAST of Bhi05G000788 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Bhi05G000788 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Bhi05G000788 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|E5GCR7\|E5GCR7_CUCME	1.1e-20	52.43	Phloem filament protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
tr\|A0A0A0K471\|A0A0A0K471_CUCSA	1.4e-20	52.43	Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G067340 PE=4 SV=1	[more]
tr\|P94012\|P94012_CUCMA	1.5e-12	47.30	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=PP1 PE=3 SV=1	[more]
tr\|Q7DMD5\|Q7DMD5_CUCMA	1.8e-10	35.16	Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=cPC7 PE=...	[more]
tr\|E5GCR6\|E5GCR6_CUCME	2.2e-08	37.89	Cysteine proteinase inhibitor OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

BLAST of Bhi05G000788 vs. NCBI nr


Match Name	E-value	Identity	Description
ADN34265.1	1.7e-20	52.43	phloem filament protein [Cucumis melo subsp. melo]	[more]
KGN43779.1	2.2e-20	52.43	Phloem filament protein [Cucumis sativus]	[more]
XP_022927698.1	5.8e-13	47.30	uncharacterized protein LOC111434515 [Cucurbita moschata]	[more]
XP_022927869.1	9.9e-13	45.68	uncharacterized protein LOC111434636 isoform X1 [Cucurbita moschata]	[more]
XP_022927870.1	9.9e-13	45.68	uncharacterized protein LOC111434636 isoform X2 [Cucurbita moschata]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027214	Cystatin
IPR009994	PP1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0030414	peptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000788	Bhi05M000788	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 11..103 e-value: 3.9E-11 score: 44.8
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 6..101
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 13..101
IPR009994	Phloem filament PP1	PFAM	PF07430	PP1	coord: 23..100 e-value: 7.7E-20 score: 70.6
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 6..101

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi05G000788	Csa7G067340	Cucumber (Chinese Long) v2	cuwgoB661
Bhi05G000788	MELO3C018579	Melon (DHL92) v3.5.1	mewgoB220
Bhi05G000788	ClCG09G016270	Watermelon (Charleston Gray)	wcgwgoB033
Bhi05G000788	CSPI07G06150	Wild cucumber (PI 183967)	cpiwgoB672
Bhi05G000788	CsGy7G005810	Cucumber (Gy14) v2	cgybwgoB632
Bhi05G000788	MELO3C018579.2	Melon (DHL92) v3.6.1	medwgoB220
Bhi05G000788	CsaV3_7G007130	Cucumber (Chinese Long) v3	cucwgoB686
Bhi05G000788	Cla012940	Watermelon (97103) v1	wgowmB243
Bhi05G000788	Cla97C09G178040	Watermelon (97103) v2	wgowmbB263

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi05G000788	Wax gourd	wgowgoB002
Bhi05G000788	Cucumber (Gy14) v1	cgywgoB445
Bhi05G000788	Cucurbita maxima (Rimu)	cmawgoB0116
Bhi05G000788	Cucurbita maxima (Rimu)	cmawgoB0642
Bhi05G000788	Cucurbita moschata (Rifu)	cmowgoB0120
Bhi05G000788	Cucurbita moschata (Rifu)	cmowgoB0642
Bhi05G000788	Cucurbita pepo (Zucchini)	cpewgoB0317
Bhi05G000788	Cucurbita pepo (Zucchini)	cpewgoB0856
Bhi05G000788	Wild cucumber (PI 183967)	cpiwgoB387
Bhi05G000788	Bottle gourd (USVL1VR-Ls)	lsiwgoB320
Bhi05G000788	Melon (DHL92) v3.6.1	medwgoB592
Bhi05G000788	Melon (DHL92) v3.5.1	mewgoB586
Bhi05G000788	Silver-seed gourd	carwgoB0377
Bhi05G000788	Silver-seed gourd	carwgoB0867