Carg08554-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg08554-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Ribosomal protein S6 family protein
Location	Cucurbita_argyrosperma_scaffold_022 : 1002896 .. 1005308 (-)
Sequence length	1195

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTAAATCAACCCAGCCGAAGTGCTTCGTACCCTCATGAACGTGATATCGGCCTGCACAAAGGTCTACTACGGGTAGAGTCTTATCCATATCTATCTTCTTGCCACTGAATTTCCGAGTCTGCCTCAACAGCAGAACTCATTCAAATTAACATGGCCTTCACTTCCACTCTTACCGCTACTTCTTCAACCTCTCCCCTCTTCTCCAAACCCCTTTCCCTTCCTCAATTCACCCCTCACATTTCCTTGCCCTTTACCCTCAGATCCTCCAAATCCCTCTTTTCTCCCGACGGAATCGGCCCTGCTTTGGGACTCGGATTATCCGTGAAAGCCCAAACTCTTGAATTTTCAGGCTCGTTCTTCCAAGGTGGGTTCGGGACTGAGGATGAACCCCCTGCTTCTCCGGGGACAGGCTTCGCTGTTGACCTTGAAGATAAGGAGGAGCCTCAATGCCCTCCTGGACTTCGGAAGTACGAATCTATGGTGGTTCTGAGGCCTGACATGTCTGAAGATGAGAGACTCGCTCTCACCCAGAAATACGAAGAGGTAGTAACATTTGAAACGTTGGTACTTTCATGTTACCTTTCCCTTCCTCCTAAATTCTCTTTTGTCTATGAGTGAAAGAAAATGCATACGAAGGAAAAGTGAAAAGTTATAGGAGAACTTTTGTATCTGTGAGTTAAGTCAATTTGTTGCTGCGTTTCAATTTTGTTGAAGATTATATACTCCTTGTGGTCTTCCTGAGAAACCACTTGCAAATGAATGAGTTAAGAGCTTTACTGAATCAGAACATTTAGACTGTAGTTTTTACAAGCTAGTTGCTTTTATGGGAATGCTAACGAGTGCGTGTAAGTAGAATTTAGCTGGCGAGTTTAGAATTTAGAATTAGTTTGTAGTCTAAATTATGGGTTTTGTTAGTCTTGATTTGTATAATATAAATTAATGTTGCTTGTCAGATTAGCTAGCTTATCGTTTGGAATAGACAAATCAACATTTTGATCTCATGTTGCACAAAACTTATCCCGAACCAACTTTTTGTGCTTGAGAATATAGTTTGGTGTTGAACGACTTTGATGTGAAAGGCACTAGCTGATTTGTTGTAAAGATGATAGTGGCAGCGTGAGACCATATTTATGAGAGAACATTCAAGAAAGGCAGGAAAGAGATGAAAGTTACCATACAATTTTGGAAAAGTTGCATGATCCTAATTGCCACATAGTTGCTTGAATGGGCAGTTCATTTCTAAAAGGGAACTGTTTGCTTGTCATGTTCTTTACAAGTATGCACACCTTATCATGAAGACATTGGAATACAAAGTTTGTGCAAAAATTTTTTTATTGCTCTTTTGATGCATGTGTAATGTTATGATAAAAAAAAGTAATATCGATTTCATCTTTGTGAAAAATTTATTTTAGCTATCCTTGATATAAGTGGAGAATTCTGGTTGTCATGTTAAAGGTTTATGTGCTGTAAGCATAAACCAATGATTTTTTTGTAGTTTTTGTTTGTTTTTTATTTTCCCGTTCAACAAGTGGAGGTGGATATTTGAGCCTCTATCTCTTGGTGAAAAACGTATTCCTTAACTAGTTTGATTGTCTTCAAGTTGGCAATGATGATCTTAGTTGATTGAATAATTTTGGTCTTCGCTCTATATAAGCAAATGATTCTTTGGTCAAAGGGGCAATCATTGGTGGATGAGAGTGAAGCTAGTTTTGTTTTCTTTCCGCATAGTGTTTGATAAAGAATGGCCCATTTTTTTTGCAGTTGCTTGTTGCTGGGGGTGGCATGTATGTAGAGGTATTCCACAAAGGGGTGGTTCCACTAGCATATAGCATCAAGAAGAAAAACAAAGCTGGTGAAACAAACACTTACTTGGATGGAATCTCTCTCCTCTTCACCTACTTCACAAAACCCGAATCAATGGGAGTTCTCGAGGCAGCGCTTCAAGCAGACGACGACGTTGTTCGATCAATGAGTTTCAAGGTAAGGAAACGAAAGTACTAAGCATGGGATTGTAATAAAATTTGTTGGTCTTTTTTCACATCAATTTTATTCAAAGCAATGGAAGAGATGTTGTAGTTGCAGTTTGTGGTTTTCCTTTCCAGAATGAGTGCCTGCTATTGTGACAACTCTTTATGGCCATTTAGGCTTTTATCCATAACCATCTGATTGGTGCTGTGTTTATGAGAGGGCTTCCAATGGAAGTGCTGTTTGAAATAGGAAAAAGGTTGGATAGAGAAAAGAAAAGGAATGTAGATTAGATTTAGATTTAGATTTAGACGTTGATCTTTCATAGCTGGAAAATCCACTTAGTGACCGAGAACCTTTTTTAATGATCCACCAATCACTGTCCCGTTCTGTCAATCGATCAACACACTCCGTTCAAAGTGCATTTGGATTGAAAAGGAAAAAAGA

mRNA sequence

CCTAAATCAACCCAGCCGAAGTGCTTCGTACCCTCATGAACGTGATATCGGCCTGCACAAAGGTCTACTACGGGTAGAGTCTTATCCATATCTATCTTCTTGCCACTGAATTTCCGAGTCTGCCTCAACAGCAGAACTCATTCAAATTAACATGGCCTTCACTTCCACTCTTACCGCTACTTCTTCAACCTCTCCCCTCTTCTCCAAACCCCTTTCCCTTCCTCAATTCACCCCTCACATTTCCTTGCCCTTTACCCTCAGATCCTCCAAATCCCTCTTTTCTCCCGACGGAATCGGCCCTGCTTTGGGACTCGGATTATCCGTGAAAGCCCAAACTCTTGAATTTTCAGGCTCGTTCTTCCAAGGTGGGTTCGGGACTGAGGATGAACCCCCTGCTTCTCCGGGGACAGGCTTCGCTGTTGACCTTGAAGATAAGGAGGAGCCTCAATGCCCTCCTGGACTTCGGAAGTACGAATCTATGGTGGTTCTGAGGCCTGACATGTCTGAAGATGAGAGACTCGCTCTCACCCAGAAATACGAAGAGTTGCTTGTTGCTGGGGGTGGCATGTATGTAGAGGTATTCCACAAAGGGGTGGTTCCACTAGCATATAGCATCAAGAAGAAAAACAAAGCTGGTGAAACAAACACTTACTTGGATGGAATCTCTCTCCTCTTCACCTACTTCACAAAACCCGAATCAATGGGAGTTCTCGAGGCAGCGCTTCAAGCAGACGACGACGTTGTTCGATCAATGAGTTTCAAGGTAAGGAAACGAAAGTACTAAGCATGGGATTGTAATAAAATTTGTTGGTCTTTTTTCACATCAATTTTATTCAAAGCAATGGAAGAGATGTTGTAGTTGCAGTTTGTGGTTTTCCTTTCCAGAATGAGTGCCTGCTATTGTGACAACTCTTTATGGCCATTTAGGCTTTTATCCATAACCATCTGATTGGTGCTGTGTTTATGAGAGGGCTTCCAATGGAAGTGCTGTTTGAAATAGGAAAAAGGTTGGATAGAGAAAAGAAAAGGAATGTAGATTAGATTTAGATTTAGATTTAGACGTTGATCTTTCATAGCTGGAAAATCCACTTAGTGACCGAGAACCTTTTTTAATGATCCACCAATCACTGTCCCGTTCTGTCAATCGATCAACACACTCCGTTCAAAGTGCATTTGGATTGAAAAGGAAAAAAGA

Coding sequence (CDS)

ATGGCCTTCACTTCCACTCTTACCGCTACTTCTTCAACCTCTCCCCTCTTCTCCAAACCCCTTTCCCTTCCTCAATTCACCCCTCACATTTCCTTGCCCTTTACCCTCAGATCCTCCAAATCCCTCTTTTCTCCCGACGGAATCGGCCCTGCTTTGGGACTCGGATTATCCGTGAAAGCCCAAACTCTTGAATTTTCAGGCTCGTTCTTCCAAGGTGGGTTCGGGACTGAGGATGAACCCCCTGCTTCTCCGGGGACAGGCTTCGCTGTTGACCTTGAAGATAAGGAGGAGCCTCAATGCCCTCCTGGACTTCGGAAGTACGAATCTATGGTGGTTCTGAGGCCTGACATGTCTGAAGATGAGAGACTCGCTCTCACCCAGAAATACGAAGAGTTGCTTGTTGCTGGGGGTGGCATGTATGTAGAGGTATTCCACAAAGGGGTGGTTCCACTAGCATATAGCATCAAGAAGAAAAACAAAGCTGGTGAAACAAACACTTACTTGGATGGAATCTCTCTCCTCTTCACCTACTTCACAAAACCCGAATCAATGGGAGTTCTCGAGGCAGCGCTTCAAGCAGACGACGACGTTGTTCGATCAATGAGTTTCAAGGTAAGGAAACGAAAGTACTAA

Protein sequence

MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKAQTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSEDERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTKPESMGVLEAALQADDDVVRSMSFKVRKRKY

Homology

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BLAST of Carg08554-RA vs. NCBI nr
Match: XP_023534494.1 (30S ribosomal protein S6 alpha, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.9 bits (1047), Expect = 2.1e-110
Identity = 208/210 (99.05%), Postives = 208/210 (99.05%), Query Frame = 0


Query: 1   MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 60
           MAFTSTLTATSSTSPL SKPLSLPQFTPHIS PFTLRSSKSLFSPDGIGPALGLGLSVKA
Sbjct: 1   MAFTSTLTATSSTSPLSSKPLSLPQFTPHISFPFTLRSSKSLFSPDGIGPALGLGLSVKA 60

Query: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120
           QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED
Sbjct: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120

Query: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180
           ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK
Sbjct: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180

Query: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESMGVLEAALQADDDVVRSMSFKVRKRKY
Sbjct: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 210

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BLAST of Carg08554-RA vs. NCBI nr
Match: XP_022958683.1 (30S ribosomal protein S6 alpha, chloroplastic [Cucurbita moschata])

HSP 1 Score: 405.2 bits (1040), Expect = 1.3e-109
Identity = 207/210 (98.57%), Postives = 207/210 (98.57%), Query Frame = 0


Query: 1   MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 60
           MAFTSTLTATSSTSPL SKPLSLPQFTPHIS PFTLRSSKSLFSPDGI PALGLGLSVKA
Sbjct: 1   MAFTSTLTATSSTSPLSSKPLSLPQFTPHISFPFTLRSSKSLFSPDGIDPALGLGLSVKA 60

Query: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120
           QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED
Sbjct: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120

Query: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180
           ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK
Sbjct: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180

Query: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESMGVLEAALQADDDVVRSMSFKVRKRKY
Sbjct: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 210

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BLAST of Carg08554-RA vs. NCBI nr
Match: XP_022996136.1 (30S ribosomal protein S6 alpha, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 399.4 bits (1025), Expect = 7.4e-108
Identity = 202/210 (96.19%), Postives = 204/210 (97.14%), Query Frame = 0


Query: 1   MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 60
           MAFTSTLTATSS SPL SKP SLPQFTPHIS PFTLRSSKSLFSPDGIGPALGLGLSVKA
Sbjct: 1   MAFTSTLTATSSASPLSSKPHSLPQFTPHISFPFTLRSSKSLFSPDGIGPALGLGLSVKA 60

Query: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120
           QTLEFSGSFFQGGFGTEDEPP SPGTGFA+DLEDKEEPQCPPGLRKYESMVVLRPDMSED
Sbjct: 61  QTLEFSGSFFQGGFGTEDEPPTSPGTGFAIDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120

Query: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180
           ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK
Sbjct: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180

Query: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESMG+LEA LQADDDVVRSMSFKVRKRKY
Sbjct: 181 PESMGILEATLQADDDVVRSMSFKVRKRKY 210

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BLAST of Carg08554-RA vs. NCBI nr
Match: XP_022156008.1 (30S ribosomal protein S6 alpha, chloroplastic [Momordica charantia])

HSP 1 Score: 321.6 bits (823), Expect = 1.9e-84
Identity = 168/210 (80.00%), Postives = 181/210 (86.19%), Query Frame = 0


Query: 1   MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 60
           MAF STLTATSSTSPL SK L LPQFT  IS    LR SKSLF P GI P        KA
Sbjct: 1   MAFASTLTATSSTSPLSSKSLFLPQFTHQIS----LRPSKSLFFPHGIAPP-------KA 60

Query: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120
           QTLEFSGSFF+ GFG+ED+PP+ PGTGFA  +EDKEEPQCPPGLRKYE+MVVLRPDMSED
Sbjct: 61  QTLEFSGSFFESGFGSEDDPPSPPGTGFAAAIEDKEEPQCPPGLRKYETMVVLRPDMSED 120

Query: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180
           +RLALTQKYEELLVAGGGMY+EVF++GV+PLAYSIKKKNKAGETNTYLDGI LLFTYFTK
Sbjct: 121 DRLALTQKYEELLVAGGGMYLEVFNRGVIPLAYSIKKKNKAGETNTYLDGIYLLFTYFTK 180

Query: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESMG+LE  LQA+DDVVRSMSFKVRKRKY
Sbjct: 181 PESMGILEETLQAEDDVVRSMSFKVRKRKY 199

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BLAST of Carg08554-RA vs. NCBI nr
Match: XP_023549667.1 (30S ribosomal protein S6 alpha, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.0 bits (811), Expect = 4.8e-83
Identity = 165/210 (78.57%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 1   MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 60
           MAFTSTL   SS+S             P +  PFTL  SK LF P+ I P+LGL LSVKA
Sbjct: 38  MAFTSTLITASSSS-------------PSLLFPFTLTPSKPLFPPNPIAPSLGLRLSVKA 97

Query: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120
           QTLEFSGSFF+GGFG EDEPPA PGTGFA  LEDKEE QCPPGLRKYESMV+LRPDMSED
Sbjct: 98  QTLEFSGSFFEGGFGAEDEPPAPPGTGFADALEDKEEXQCPPGLRKYESMVILRPDMSED 157

Query: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180
           ERLALTQKYEELLVAGGGMYVEVF++G+VPLAYSIKKKNKAGETNTY+DGI+LLFTYFTK
Sbjct: 158 ERLALTQKYEELLVAGGGMYVEVFNRGLVPLAYSIKKKNKAGETNTYMDGINLLFTYFTK 217

Query: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESM VLEAALQADDDV+RSMSFKVR RKY
Sbjct: 218 PESMRVLEAALQADDDVIRSMSFKVRNRKY 234

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BLAST of Carg08554-RA vs. TAIR10
Match: AT1G64510.1 (Translation elongation factor EF1B/ribosomal protein S6 family protein)

HSP 1 Score: 245.0 bits (624), Expect = 4.2e-65
Identity = 126/193 (65.28%), Postives = 149/193 (77.20%), Query Frame = 0


Query: 20  PLSLPQFTPHISLPFTLRS--SKSLFSPDGIGPALGLGLSVKAQTLEFSGSFFQGGFGTE 79
           PL      P +S   +LR   SKS      I    G    VK+Q L+FSG+FF+GGFG++
Sbjct: 14  PLPNVSSQPLLSFSHSLRPFISKSKPMCASIQKRDGSQFVVKSQALDFSGTFFEGGFGSD 73

Query: 80  DEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSEDERLALTQKYEELLVAGG 139
           D+P +  G+G +  LEDK EPQCPPGLR+YE+M VLRPDMSEDERL LTQKYEELLVAGG
Sbjct: 74  DDPTSPSGSGVSTALEDKPEPQCPPGLRQYETMAVLRPDMSEDERLGLTQKYEELLVAGG 133

Query: 140 GMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTKPESMGVLEAALQADDDV 199
           GMYVEVF++GV+PLAYSI+KKNKAGETNTYLDGI LLFTYFTKPES+  LE  L ADDD+
Sbjct: 134 GMYVEVFNRGVIPLAYSIRKKNKAGETNTYLDGIYLLFTYFTKPESIVPLETVLTADDDI 193

Query: 200 VRSMSFKVRKRKY 211
           +RS SFK++KRKY
Sbjct: 194 IRSSSFKIKKRKY 206

[Close]

BLAST of Carg08554-RA vs. Swiss-Prot
Match: sp|Q8VY91|RR6_ARATH (30S ribosomal protein S6 alpha, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPS6 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 7.6e-64
Identity = 126/193 (65.28%), Postives = 149/193 (77.20%), Query Frame = 0


Query: 20  PLSLPQFTPHISLPFTLRS--SKSLFSPDGIGPALGLGLSVKAQTLEFSGSFFQGGFGTE 79
           PL      P +S   +LR   SKS      I    G    VK+Q L+FSG+FF+GGFG++
Sbjct: 14  PLPNVSSQPLLSFSHSLRPFISKSKPMCASIQKRDGSQFVVKSQALDFSGTFFEGGFGSD 73

Query: 80  DEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSEDERLALTQKYEELLVAGG 139
           D+P +  G+G +  LEDK EPQCPPGLR+YE+M VLRPDMSEDERL LTQKYEELLVAGG
Sbjct: 74  DDPTSPSGSGVSTALEDKPEPQCPPGLRQYETMAVLRPDMSEDERLGLTQKYEELLVAGG 133

Query: 140 GMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTKPESMGVLEAALQADDDV 199
           GMYVEVF++GV+PLAYSI+KKNKAGETNTYLDGI LLFTYFTKPES+  LE  L ADDD+
Sbjct: 134 GMYVEVFNRGVIPLAYSIRKKNKAGETNTYLDGIYLLFTYFTKPESIVPLETVLTADDDI 193

Query: 200 VRSMSFKVRKRKY 211
           +RS SFK++KRKY
Sbjct: 194 IRSSSFKIKKRKY 206

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BLAST of Carg08554-RA vs. Swiss-Prot
Match: sp|P82403|RR6_SPIOL (30S ribosomal protein S6 alpha, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPS6 PE=1 SV=3)

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-60
Identity = 131/211 (62.09%), Postives = 152/211 (72.04%), Query Frame = 0


Query: 2   AFTSTLTATSSTSPLFSKPLSLPQFTPH-ISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 61
           + TSTL ++S T+ L S P   P+  P  I+    L                     VKA
Sbjct: 5   SLTSTLPSSSPTTSLHSIP--KPKLKPSTINFTHNLNPFNXXXXXXXXXXXXXXXPYVKA 64

Query: 62  QTLEFSGSFFQGGFGTEDE-PPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSE 121
             L+FSGSFF+GGFG  DE PP++P  G AV  E+K EPQCPPGLR+YE+M VLRPDM+E
Sbjct: 65  IALDFSGSFFEGGFGGLDEDPPSTPPAGLAV--EEKPEPQCPPGLRQYETMAVLRPDMTE 124

Query: 122 DERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFT 181
           DERL LTQKYEELLVAGG MYVEVF++GV+PLAYSIK+KNKAGETN YLDGI LLFTYFT
Sbjct: 125 DERLTLTQKYEELLVAGGAMYVEVFNRGVIPLAYSIKRKNKAGETNNYLDGIYLLFTYFT 184

Query: 182 KPESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           KPES+  LEAAL  DDDV+RS SFK+RKRKY
Sbjct: 185 KPESISPLEAALVTDDDVIRSSSFKIRKRKY 211

[Close]

BLAST of Carg08554-RA vs. Swiss-Prot
Match: sp|Q6B922|RR6_GRATL (30S ribosomal protein S6, chloroplastic OS=Gracilaria tenuistipitata var. liui OX=285951 GN=rps6 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 34/101 (33.66%), Postives = 54/101 (53.47%), Query Frame = 0


Query: 104 LRKYESMVVLRPDMSEDERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGE 163
           L KYE++ +L+P+++E E L L  +Y+ L+   GG  + V HKG   L Y+IK       
Sbjct: 3   LNKYETIYILQPNITESENLILINEYKALIKKYGGNNIVVQHKGRRHLNYNIK------- 62

Query: 164 TNTYLDGISLLFTYFTKPESMGVLEAALQADDDVVRSMSFK 205
             +Y DGI +   Y      + +LE A++    V+R M+ K
Sbjct: 63  --SYYDGIYVQINYTASSNLVRILEKAMRLSQHVIRYMTIK 94

[Close]

BLAST of Carg08554-RA vs. Swiss-Prot
Match: sp|Q10XV0|RS6_TRIEI (30S ribosomal protein S6 OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=rpsF PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 7.7e-08
Identity = 30/98 (30.61%), Postives = 53/98 (54.08%), Query Frame = 0


Query: 107 YESMVVLRPDMSEDERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNT 166
           YE+M +LRPDM+E++      KY+ +L   G   +++ H+G + LAY         E N 
Sbjct: 6   YETMYILRPDMTEEKVEQAITKYKSMLEEQGAYDIKIQHRGKLRLAY---------EINN 65

Query: 167 YLDGISLLFTYFTKPESMGVLEAALQADDDVVRSMSFK 205
             +GI +   Y   P  + +LE A++  ++V+R ++ K
Sbjct: 66  QREGIYIQMNYQGPPTQIAILERAMRLSEEVIRYLTVK 94

[Close]

BLAST of Carg08554-RA vs. Swiss-Prot
Match: sp|A5GX14|RS6_SYNR3 (30S ribosomal protein S6 OS=Synechococcus sp. (strain RCC307) OX=316278 GN=rpsF PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.0e-07
Identity = 32/98 (32.65%), Postives = 55/98 (56.12%), Query Frame = 0


Query: 107 YESMVVLRPDMSEDERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNT 166
           YE+M +LRPD++E+E  +   KY +L+V  GG  ++   +G   LAY+I K         
Sbjct: 7   YETMYILRPDIAEEEVESHANKYRDLVVEAGGEVIDSQMRGKRRLAYNIGK--------- 66

Query: 167 YLDGISLLFTYFTKPESMGVLEAALQADDDVVRSMSFK 205
           + +GI +   Y    + +G LE A++  +DV+R ++ K
Sbjct: 67  HREGIYIQLNYSGDGKQVGPLEKAMRLSEDVIRYLTVK 95

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BLAST of Carg08554-RA vs. TrEMBL
Match: tr|A0A1S4DT54|A0A1S4DT54_CUCME (30S ribosomal protein S6 alpha, chloroplastic OS=Cucumis melo OX=3656 GN=LOC107990347 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.9e-81
Identity = 163/210 (77.62%), Postives = 175/210 (83.33%), Query Frame = 0


Query: 1   MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 60
           MAFTSTLTATSSTSPL SK  SLP FT  IS PFTL+ SK LFSPDGIG ALG+GLSVKA
Sbjct: 1   MAFTSTLTATSSTSPLSSKSPSLPPFTNQISFPFTLKLSKPLFSPDGIGSALGIGLSVKA 60

Query: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120
           QTLEFSGSFF+GG G++DEPPA P                   LRKYE+MVVLRPDMSED
Sbjct: 61  QTLEFSGSFFEGGLGSQDEPPAPPXXXXXXXXXXXXXXXXXXXLRKYETMVVLRPDMSED 120

Query: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180
           ERLA T+KYEELLVAGG MYVEVF+KG++PLAYSIKKKNKAGETNTYLDGI+LLFTYFTK
Sbjct: 121 ERLAFTEKYEELLVAGGAMYVEVFNKGLIPLAYSIKKKNKAGETNTYLDGINLLFTYFTK 180

Query: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESM VLEA LQADDDVVRSMSFKVRKRK+
Sbjct: 181 PESMAVLEATLQADDDVVRSMSFKVRKRKF 210

[Close]

BLAST of Carg08554-RA vs. TrEMBL
Match: tr|A0A0A0KK24|A0A0A0KK24_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148810 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.1e-79
Identity = 162/210 (77.14%), Postives = 172/210 (81.90%), Query Frame = 0


Query: 1   MAFTSTLTATSSTSPLFSKPLSLPQFTPHISLPFTLRSSKSLFSPDGIGPALGLGLSVKA 60
           MAFTSTLTATSSTSPL SK   L  FT  IS PF L+ SK LFSP GIGPALGLGLSVKA
Sbjct: 1   MAFTSTLTATSSTSPLSSKSPFLTPFTNQISFPFALKLSKPLFSPYGIGPALGLGLSVKA 60

Query: 61  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 120
           QTLEFSGSFF+GG G++DEPPA P                   LRKYESMVVLRPDMSED
Sbjct: 61  QTLEFSGSFFEGGLGSQDEPPAPPXXXXXXXXXXXXXXXXXXXLRKYESMVVLRPDMSED 120

Query: 121 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 180
           ERLA T+KYEELLVAGG MYVEVF+KG++PLAYSIKKKNKAGETNTYLDGI+LLFTYFTK
Sbjct: 121 ERLAFTEKYEELLVAGGAMYVEVFNKGLIPLAYSIKKKNKAGETNTYLDGINLLFTYFTK 180

Query: 181 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESM VLEA LQADDDVVRSMSFKVRKRK+
Sbjct: 181 PESMAVLEATLQADDDVVRSMSFKVRKRKF 210

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BLAST of Carg08554-RA vs. TrEMBL
Match: tr|A0A2P5VQH8|A0A2P5VQH8_GOSBA (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA39614 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.7e-68
Identity = 146/210 (69.52%), Postives = 165/210 (78.57%), Query Frame = 0


Query: 11  SSTSPLFSKPLS---LPQFTPHISLPFT------LRSSKSLFS-PDGIGPALGLGLSVKA 70
           S TS L + P     LPQF+P   L FT      L   KSL S      P+    L+VKA
Sbjct: 3   SPTSALTNSPFCPHLLPQFSPLPVLSFTHSLKPILPKLKSLISLKRDNHPST---LTVKA 62

Query: 71  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 130
           QTL+FSGSFF+GGFG++D+PP+ PG+G    LEDKEEPQCPPGLR+YE+M VLRPDMSED
Sbjct: 63  QTLDFSGSFFEGGFGSDDDPPSQPGSGITA-LEDKEEPQCPPGLRQYETMAVLRPDMSED 122

Query: 131 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 190
           ERLALTQKYEELLVAGGGMY+EVF++GV+PLAYSIKKKNKAGETNTYLDGI LLFTYFTK
Sbjct: 123 ERLALTQKYEELLVAGGGMYIEVFNRGVIPLAYSIKKKNKAGETNTYLDGIYLLFTYFTK 182

Query: 191 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESM  LEAAL  DDDV+RS SFK+RKRKY
Sbjct: 183 PESMTALEAALNTDDDVIRSTSFKIRKRKY 208

[Close]

BLAST of Carg08554-RA vs. TrEMBL
Match: tr|A0A1U8NDZ2|A0A1U8NDZ2_GOSHI (30S ribosomal protein S6 alpha, chloroplastic-like OS=Gossypium hirsutum OX=3635 GN=LOC107947179 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.7e-68
Identity = 146/210 (69.52%), Postives = 165/210 (78.57%), Query Frame = 0


Query: 11  SSTSPLFSKPLS---LPQFTPHISLPFT------LRSSKSLFS-PDGIGPALGLGLSVKA 70
           S TS L + P     LPQF+P   L FT      L   KSL S      P+    L+VKA
Sbjct: 3   SPTSALTNSPFCPHLLPQFSPLPVLSFTHSLKPILPKLKSLISLKRDNHPST---LTVKA 62

Query: 71  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 130
           QTL+FSGSFF+GGFG++D+PP+ PG+G    LEDKEEPQCPPGLR+YE+M VLRPDMSED
Sbjct: 63  QTLDFSGSFFEGGFGSDDDPPSQPGSGITA-LEDKEEPQCPPGLRQYETMAVLRPDMSED 122

Query: 131 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 190
           ERLALTQKYEELLVAGGGMY+EVF++GV+PLAYSIKKKNKAGETNTYLDGI LLFTYFTK
Sbjct: 123 ERLALTQKYEELLVAGGGMYIEVFNRGVIPLAYSIKKKNKAGETNTYLDGIYLLFTYFTK 182

Query: 191 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESM  LEAAL  DDDV+RS SFK+RKRKY
Sbjct: 183 PESMTALEAALNTDDDVIRSTSFKIRKRKY 208

[Close]

BLAST of Carg08554-RA vs. TrEMBL
Match: tr|A0A0B0PSF5|A0A0B0PSF5_GOSAR (30S ribosomal S6 alpha, chloroplastic-like protein OS=Gossypium arboreum OX=29729 GN=F383_05526 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.9e-68
Identity = 144/210 (68.57%), Postives = 165/210 (78.57%), Query Frame = 0


Query: 11  SSTSPLFSKPLS---LPQFTPHISLPFTLRSSKSLFSPDGIGPALGL-------GLSVKA 70
           S TS L + P     LPQF+P   L FT  S KS+  P  +   + L        L+VKA
Sbjct: 3   SPTSALTNSPFCPHLLPQFSPLPVLSFT-HSLKSI--PPKLKSLISLKRDNHPSTLTVKA 62

Query: 71  QTLEFSGSFFQGGFGTEDEPPASPGTGFAVDLEDKEEPQCPPGLRKYESMVVLRPDMSED 130
           QTL+FSGSFF+GGFG++D+PP+ PG+G    LEDKEEPQCPPGLR+YE+M VLRPDMSED
Sbjct: 63  QTLDFSGSFFEGGFGSDDDPPSQPGSGITA-LEDKEEPQCPPGLRQYETMAVLRPDMSED 122

Query: 131 ERLALTQKYEELLVAGGGMYVEVFHKGVVPLAYSIKKKNKAGETNTYLDGISLLFTYFTK 190
           ERLALTQKYEELLVAGGGMY+EVF++GV+PLAYSIKKKNKAGETN YLDGI LLFTYFTK
Sbjct: 123 ERLALTQKYEELLVAGGGMYIEVFNRGVIPLAYSIKKKNKAGETNAYLDGIYLLFTYFTK 182

Query: 191 PESMGVLEAALQADDDVVRSMSFKVRKRKY 211
           PESM  LEAAL  DDDV+RS SFK+RKRKY
Sbjct: 183 PESMTALEAALNTDDDVIRSTSFKIRKRKY 208

The following BLAST results are available for this feature:

BLAST of Carg08554-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023534494.1	2.1e-110	99.05	30S ribosomal protein S6 alpha, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022958683.1	1.3e-109	98.57	30S ribosomal protein S6 alpha, chloroplastic [Cucurbita moschata]	[more]
XP_022996136.1	7.4e-108	96.19	30S ribosomal protein S6 alpha, chloroplastic-like [Cucurbita maxima]	[more]
XP_022156008.1	1.9e-84	80.00	30S ribosomal protein S6 alpha, chloroplastic [Momordica charantia]	[more]
XP_023549667.1	4.8e-83	78.57	30S ribosomal protein S6 alpha, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg08554-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G64510.1	4.2e-65	65.28	Translation elongation factor EF1B/ribosomal protein S6 family protein	[more]

BLAST of Carg08554-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8VY91\|RR6_ARATH	7.6e-64	65.28	30S ribosomal protein S6 alpha, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
sp\|P82403\|RR6_SPIOL	1.3e-60	62.09	30S ribosomal protein S6 alpha, chloroplastic OS=Spinacia oleracea OX=3562 GN=RP...	[more]
sp\|Q6B922\|RR6_GRATL	2.0e-08	33.66	30S ribosomal protein S6, chloroplastic OS=Gracilaria tenuistipitata var. liui O...	[more]
sp\|Q10XV0\|RS6_TRIEI	7.7e-08	30.61	30S ribosomal protein S6 OS=Trichodesmium erythraeum (strain IMS101) OX=203124 G...	[more]
sp\|A5GX14\|RS6_SYNR3	1.0e-07	32.65	30S ribosomal protein S6 OS=Synechococcus sp. (strain RCC307) OX=316278 GN=rpsF ...	[more]

BLAST of Carg08554-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4DT54\|A0A1S4DT54_CUCME	3.9e-81	77.62	30S ribosomal protein S6 alpha, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1079...	[more]
tr\|A0A0A0KK24\|A0A0A0KK24_CUCSA	2.1e-79	77.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148810 PE=3 SV=1	[more]
tr\|A0A2P5VQH8\|A0A2P5VQH8_GOSBA	1.7e-68	69.52	Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA39614 PE=3 SV...	[more]
tr\|A0A1U8NDZ2\|A0A1U8NDZ2_GOSHI	1.7e-68	69.52	30S ribosomal protein S6 alpha, chloroplastic-like OS=Gossypium hirsutum OX=3635...	[more]
tr\|A0A0B0PSF5\|A0A0B0PSF5_GOSAR	2.9e-68	68.57	30S ribosomal S6 alpha, chloroplastic-like protein OS=Gossypium arboreum OX=2972...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome
GO:0019843	rRNA binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR035980	Ribosomal_S6_sf
IPR020814	Ribosomal_S6_plastid/chlpt
IPR014717	Transl_elong_EF1B/ribosomal_S6
IPR000529	Ribosomal_S6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
biological_process	GO:0042254	ribosome biogenesis
cellular_component	GO:0005840	ribosome
molecular_function	GO:0019843	rRNA binding
molecular_function	GO:0003735	structural constituent of ribosome

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg08554	Carg08554	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg08554-RA	Carg08554-RA-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08554-RA.three_prime_UTR.1	Carg08554-RA.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08554-RA.CDS.2	Carg08554-RA.CDS.2	CDS
Carg08554-RA.CDS.1	Carg08554-RA.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08554-RA.five_prime_UTR.1	Carg08554-RA.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08554-RA.exon.2	Carg08554-RA.exon.2	exon
Carg08554-RA.exon.1	Carg08554-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000529	Ribosomal protein S6	PFAM	PF01250	Ribosomal_S6	coord: 106..204 e-value: 1.1E-15 score: 57.5
IPR000529	Ribosomal protein S6	PANTHER	PTHR21011	MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6	coord: 55..210
IPR014717	Translation elongation factor EF1B/ribosomal protein S6	GENE3D	G3DSA:3.30.70.60		coord: 104..210 e-value: 8.3E-16 score: 59.8
None	No IPR available	PANTHER	PTHR21011:SF7	30S RIBOSOMAL PROTEIN S6 ALPHA, CHLOROPLASTIC	coord: 55..210
None	No IPR available	CDD	cd15487	bS6_chloro_cyano	coord: 104..205 e-value: 3.37706E-33 score: 115.383
IPR020814	Ribosomal protein S6, plastid/chloroplast	HAMAP	MF_00360	Ribosomal_S6	coord: 104..207 score: 13.645
IPR035980	Ribosomal protein S6 superfamily	SUPERFAMILY	SSF54995	Ribosomal protein S6	coord: 104..208