Carg27183-RA (mRNA) Silver-seed gourd

NameCarg27183-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionE3 ubiquitin-protein ligase RHY1A
LocationCucurbita_argyrosperma_scaffold_486 : 8900 .. 9626 (+)
Sequence length500
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTCCAATCACTGAACTTAACTACCAATTCCATATCAAACCGATAAAGCCCTAGATCAGTGCAGGAACTTCAGCTTTCAAGGTTTCGCCAAGTTTCTCCCATGGCCGGAATGCTTCCAGGTGTAGAGTGTGCTCGAAGAAGACGGTTACATCAAACCAAAGCATGCTCGGATTCACCGCCCATCGTCGCCACTCGGACCTTAACCAGGAAATCCGTTCTCTGCTTGTACACAAGCAACCACGAAACGCTCCTCGCTTCAAATCCCTCTCTGGTTCGTAAATCGCATCTCGACCTGTCAATGAATTAACAAATTATTCATTCGATTGAGGATGATGAACAGAGCTGATTCGATTTATCGTGTTTTTTTTTTCTTTTGCAGCAAGGAAGCCTGCTAGTCGAGGCACAAGAAAACGATAATCTGGACGGAATCGCGAGAGAGGCGAAGAGGAGATTGGACGAGCGATTGAGGAAATCAGAGGATAAAAGGTACTGAAACAACGGCGTCCGGAACAGCCGCGATGAATTTACATTTCGTTAATTTATTAATCATAAAACCTAAACGAAACACATCCAAAATTACTCTTGTAATCTGCGATTGGATGCAGAGGGATAGCGACGAGAAAGTTCAGTTGGACGAATTGGATCGCCGGCGAGCAAAACAATTGTGGTCAGTGCTGGTGATGAAGACAACTTCATTCCAGTGGCTCCTTCTTCTTCTTCTTCTT

mRNA sequence

TCTTTCCAATCACTGAACTTAACTACCAATTCCATATCAAACCGATAAAGCCCTAGATCAGTGCAGGAACTTCAGCTTTCAAGGTTTCGCCAAGTTTCTCCCATGGCCGGAATGCTTCCAGGTGTAGAGTGTGCTCGAAGAAGACGGTTACATCAAACCAAAGCATGCTCGGATTCACCGCCCATCGTCGCCACTCGGACCTTAACCAGGAAATCCGTTCTCTGCTTGTACACAAGCAACCACGAAACGCTCCTCGCTTCAAATCCCTCTCTGCAAGGAAGCCTGCTAGTCGAGGCACAAGAAAACGATAATCTGGACGGAATCGCGAGAGAGGCGAAGAGGAGATTGGACGAGCGATTGAGGAAATCAGAGGATAAAAGAGGGATAGCGACGAGAAAGTTCAGTTGGACGAATTGGATCGCCGGCGAGCAAAACAATTGTGGTCAGTGCTGGTGATGAAGACAACTTCATTCCAGTGGCTCCTTCTTCTTCTTCTTCTT

Coding sequence (CDS)

ATGGCCGGAATGCTTCCAGGTGTAGAGTGTGCTCGAAGAAGACGGTTACATCAAACCAAAGCATGCTCGGATTCACCGCCCATCGTCGCCACTCGGACCTTAACCAGGAAATCCGTTCTCTGCTTGTACACAAGCAACCACGAAACGCTCCTCGCTTCAAATCCCTCTCTGCAAGGAAGCCTGCTAGTCGAGGCACAAGAAAACGATAATCTGGACGGAATCGCGAGAGAGGCGAAGAGGAGATTGGACGAGCGATTGAGGAAATCAGAGGATAAAAGAGGGATAGCGACGAGAAAGTTCAGTTGGACGAATTGGATCGCCGGCGAGCAAAACAATTGTGGTCAGTGCTGGTGA

Protein sequence

MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGSLLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW
BLAST of Carg27183-RA vs. NCBI nr
Match: XP_022933256.1 (uncharacterized protein LOC111440241 [Cucurbita moschata])

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-59
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS
Sbjct: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW 118
           LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW
Sbjct: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW 117

BLAST of Carg27183-RA vs. NCBI nr
Match: XP_023531367.1 (uncharacterized protein LOC111793636 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235.7 bits (600), Expect = 7.8e-59
Identity = 115/117 (98.29%), Postives = 116/117 (99.15%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRRLHQTKACSDSPPI ATRTLTRKSVLCLYTSNHETLLASNPSLQGS
Sbjct: 1   MAGMLPGVECARRRRLHQTKACSDSPPIAATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW 118
           LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATR+FSWTNWIAGEQNNCGQCW
Sbjct: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRRFSWTNWIAGEQNNCGQCW 117

BLAST of Carg27183-RA vs. NCBI nr
Match: XP_023005576.1 (uncharacterized protein LOC111498516 isoform X1 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 3.0e-58
Identity = 113/117 (96.58%), Postives = 116/117 (99.15%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRRLHQTKACSDSPPI ATRTLTRKS+LCLYTSNHETLLASNPSLQGS
Sbjct: 32  MAGMLPGVECARRRRLHQTKACSDSPPIAATRTLTRKSILCLYTSNHETLLASNPSLQGS 91

Query: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW 118
           LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRG+ATRKFSWTNWIAGE+NNCGQCW
Sbjct: 92  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGMATRKFSWTNWIAGEKNNCGQCW 148

BLAST of Carg27183-RA vs. NCBI nr
Match: XP_023005584.1 (uncharacterized protein LOC111498516 isoform X2 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 3.0e-58
Identity = 113/117 (96.58%), Postives = 116/117 (99.15%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRRLHQTKACSDSPPI ATRTLTRKS+LCLYTSNHETLLASNPSLQGS
Sbjct: 21  MAGMLPGVECARRRRLHQTKACSDSPPIAATRTLTRKSILCLYTSNHETLLASNPSLQGS 80

Query: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW 118
           LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRG+ATRKFSWTNWIAGE+NNCGQCW
Sbjct: 81  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGMATRKFSWTNWIAGEKNNCGQCW 137

BLAST of Carg27183-RA vs. NCBI nr
Match: XP_023005591.1 (uncharacterized protein LOC111498516 isoform X3 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 3.0e-58
Identity = 113/117 (96.58%), Postives = 116/117 (99.15%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRRLHQTKACSDSPPI ATRTLTRKS+LCLYTSNHETLLASNPSLQGS
Sbjct: 1   MAGMLPGVECARRRRLHQTKACSDSPPIAATRTLTRKSILCLYTSNHETLLASNPSLQGS 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGIATRKFSWTNWIAGEQNNCGQCW 118
           LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRG+ATRKFSWTNWIAGE+NNCGQCW
Sbjct: 61  LLVEAQENDNLDGIAREAKRRLDERLRKSEDKRGMATRKFSWTNWIAGEKNNCGQCW 117

BLAST of Carg27183-RA vs. TAIR10
Match: AT2G15580.1 (RING/U-box superfamily protein)

HSP 1 Score: 65.1 bits (157), Expect = 3.3e-11
Identity = 44/100 (44.00%), Postives = 58/100 (58.00%), Query Frame = 0

Query: 1  MAGMLPGVECARRRRLHQTKACSDSPPIVATRTL----------TRKSVLCLYTSNHETL 60
          MAGMLPGVECARRRR H       +PPI ++ T           TR+    LYT+NHE+ 
Sbjct: 1  MAGMLPGVECARRRRFH-----GGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESH 60

Query: 61 LASNPSLQGSLLVEAQENDN---LDGIAREAKRRLDERLR 88
           A     + S+  ++   DN   LDG A+EAK+RL++RLR
Sbjct: 61 QAHVSFSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLR 95

BLAST of Carg27183-RA vs. TrEMBL
Match: tr|A0A1S3B9W3|A0A1S3B9W3_CUCME (uncharacterized protein LOC103487385 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487385 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 7.5e-34
Identity = 85/124 (68.55%), Postives = 93/124 (75.00%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRR HQ K  SDSP + A + LTR+SVLCLYTSNHE LL SNPS+Q  
Sbjct: 1   MAGMLPGVECARRRRCHQNKIWSDSPQVDA-QVLTRRSVLCLYTSNHEALLVSNPSMQRR 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERL----RKSEDK-RGIATRKFSW--TNWIAGEQNNC 118
           LL+EA   D LDGIAREAKRRLDERL    RKSED  R +  RKFSW   NWI  E++ C
Sbjct: 61  LLMEAGGEDELDGIAREAKRRLDERLWRTQRKSEDNGREMMRRKFSWRKLNWIGVEEDKC 120

BLAST of Carg27183-RA vs. TrEMBL
Match: tr|A0A1S3B9Y1|A0A1S3B9Y1_CUCME (uncharacterized protein LOC103487385 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487385 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.2e-32
Identity = 84/124 (67.74%), Postives = 93/124 (75.00%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRR HQ K  SDSP + A + LTR+SVLCLYTSNHE LL SNPS++  
Sbjct: 1   MAGMLPGVECARRRRCHQNKIWSDSPQVDA-QVLTRRSVLCLYTSNHEALLVSNPSMR-R 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERL----RKSEDK-RGIATRKFSW--TNWIAGEQNNC 118
           LL+EA   D LDGIAREAKRRLDERL    RKSED  R +  RKFSW   NWI  E++ C
Sbjct: 61  LLMEAGGEDELDGIAREAKRRLDERLWRTQRKSEDNGREMMRRKFSWRKLNWIGVEEDKC 120

BLAST of Carg27183-RA vs. TrEMBL
Match: tr|A0A0A0LWJ8|A0A0A0LWJ8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G265120 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.5e-31
Identity = 80/123 (65.04%), Postives = 92/123 (74.80%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAG LPGVECARRRR HQ    SDS P++A + LTR+SVLCLYTSN+E LL SNPS++  
Sbjct: 1   MAGTLPGVECARRRRCHQNSIWSDS-PLIAAQVLTRRSVLCLYTSNNEALLFSNPSMERR 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERL----RKSEDK-RGIATRKFSW--TNWIAGEQNNC 117
           LL+EA+  D LDGIAREAKRRLDERL    RKSED  R +  RKFSW   NWI  E++ C
Sbjct: 61  LLMEAEGEDELDGIAREAKRRLDERLLRTQRKSEDNGREMMRRKFSWRKLNWIGVEEDKC 120

BLAST of Carg27183-RA vs. TrEMBL
Match: tr|A0A2I4HNL2|A0A2I4HNL2_9ROSI (probable E3 ubiquitin-protein ligase RHY1A OS=Juglans regia OX=51240 GN=LOC109019824 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 4.0e-19
Identity = 67/146 (45.89%), Postives = 79/146 (54.11%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRR HQ+ ACSDSP  VA    TR+S  CLY SNHE    S  S + S
Sbjct: 1   MAGMLPGVECARRRRFHQSGACSDSPSTVAALGGTRRSSFCLYRSNHEPQHTSTSSQKRS 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERL---RKSEDK----------RGIA----------- 117
           LL +   ++NL  +AREAK RLDERL   RKS+ K          R I            
Sbjct: 61  LLNQVYRDENLGEVAREAKERLDERLRTQRKSKTKSTQSLSCVEGRSIVLEELNTEMFGF 120

BLAST of Carg27183-RA vs. TrEMBL
Match: tr|A0A2I4FQA0|A0A2I4FQA0_9ROSI (probable E3 ubiquitin-protein ligase RHY1A OS=Juglans regia OX=51240 GN=LOC109001127 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.9e-19
Identity = 66/147 (44.90%), Postives = 83/147 (56.46%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRLHQTKACSDSPPIVATRTLTRKSVLCLYTSNHETLLASNPSLQGS 60
           MAGMLPGVECARRRR HQ+ AC+DS   VA    TR+S  CLY SNHE    ++ SL+ S
Sbjct: 1   MAGMLPGVECARRRRFHQSGACADSQITVAAHGGTRRSSFCLYRSNHEPQHHNSTSLKRS 60

Query: 61  LLVEAQENDNLDGIAREAKRRLDERLR-------KSED---------------------- 117
            L +A +++ L  +AREAK RLDERLR       KS++                      
Sbjct: 61  SLNQAHQDEKLGEVAREAKERLDERLRTQRKTNTKSKESMKCVEGRSMVLGELQTEVFGT 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022933256.11.2e-59100.00uncharacterized protein LOC111440241 [Cucurbita moschata][more]
XP_023531367.17.8e-5998.29uncharacterized protein LOC111793636 [Cucurbita pepo subsp. pepo][more]
XP_023005576.13.0e-5896.58uncharacterized protein LOC111498516 isoform X1 [Cucurbita maxima][more]
XP_023005584.13.0e-5896.58uncharacterized protein LOC111498516 isoform X2 [Cucurbita maxima][more]
XP_023005591.13.0e-5896.58uncharacterized protein LOC111498516 isoform X3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G15580.13.3e-1144.00RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
tr|A0A1S3B9W3|A0A1S3B9W3_CUCME7.5e-3468.55uncharacterized protein LOC103487385 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
tr|A0A1S3B9Y1|A0A1S3B9Y1_CUCME3.2e-3267.74uncharacterized protein LOC103487385 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
tr|A0A0A0LWJ8|A0A0A0LWJ8_CUCSA3.5e-3165.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G265120 PE=4 SV=1[more]
tr|A0A2I4HNL2|A0A2I4HNL2_9ROSI4.0e-1945.89probable E3 ubiquitin-protein ligase RHY1A OS=Juglans regia OX=51240 GN=LOC10901... [more]
tr|A0A2I4FQA0|A0A2I4FQA0_9ROSI8.9e-1944.90probable E3 ubiquitin-protein ligase RHY1A OS=Juglans regia OX=51240 GN=LOC10900... [more]
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg27183Carg27183gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg27183-RACarg27183-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27183-RA.exon.1Carg27183-RA.exon.1exon
Carg27183-RA.exon.2Carg27183-RA.exon.2exon
Carg27183-RA.exon.3Carg27183-RA.exon.3exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27183-RA.five_prime_UTR.1Carg27183-RA.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27183-RA.CDS.1Carg27183-RA.CDS.1CDS
Carg27183-RA.CDS.2Carg27183-RA.CDS.2CDS
Carg27183-RA.CDS.3Carg27183-RA.CDS.3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27183-RA.three_prime_UTR.1Carg27183-RA.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR14155RING FINGER DOMAIN-CONTAININGcoord: 2..98
NoneNo IPR availablePANTHERPTHR14155:SF215SUBFAMILY NOT NAMEDcoord: 2..98