Carg25150-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25150-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cytochrome P450, putative
Location	Cucurbita_argyrosperma_scaffold_200 : 162507 .. 165434 (+)
Sequence length	687

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTCCGATTCGGCTCACGCCTTGTCGTACTCATATCCTCCCCCTCAGCCGTTCAAGAATGCTTCACAAAAAACGACATCATTCTCGCAAATCGCCCATTGCTCGACACCGGAAAGTACTTAGCATACAACCACACAACCCTCGCCGTCTCACCTTACGGCGAGCACTGGCGCAACCTCCGCCGGATTTGCTCCCTCGAGATATTCTCAACCAACCGCCTCAATATGTTCGTGGGTATCCGAGAAAATGAAGTGAAACGGTTGCTGAGGAAGTTATGTGGGGATTGGTCGGATGAGTTTAAGGAAGTGGAAGTGGAGCCAATGTTGTCGGATCTTACTTTCAACGTTGTTATGGGAATGGTGGGTGGAAGGAGGTTTTATGATGAGGGGTATTCGAAGAAGTTTAGAGATTTGGTGACACAAATTATGGCGCATGGTGGGTCATCGAATCCGGGTGATTTCATACCCTTGTGGAATTGGGTTGATCCAACTGGTTTTAAGAAGAGGATAATGAATCTTGCACGAAGAGGAGATGAACTTCTTCAAGAACTAATTGATGAAGCTCGAGATGGAAAGGATGGAGGGAACACTATGATTCATCATTTACTTTGCTTGCAGAAAGATGAGCCGGAGTATTATAGTGACCTTATTATCAAAGGACTTATTCAAGTACTTCTCTCTCCCTCTCTACTCTTTTCTTTATGTTTTTTTTTTCTTTTTAAGTTCACAAGTTCATGAATGTTGTGGGCATATATTGACATTTATTTGATTCATAACTCTGATTTAGTTCATAACCCTCTCTCATTTTGATTCATGAATCATCTGTGCCTATATAAAGAGATGTATTGAGTTTGATTGAGTTTGAATGTTATTTTGTATTTGTTGAGTGACATTTGGGAGGAACTTTGTTGAGAGATAGTGTGAGCGTGTGAGATATACACTTCGTAAATATTTTGATTATTGAGATTGGTTGCTATCCGTGGATATAAGGGAATTTCTTCGCTCTAACCTAAGTAAATTTTTTGGAGGAACTTTGTTAAGAGATTTTGTAGTGTGAGAGTGTGAAATATAGACTTTATAAACACTTTCATTATTGAGAATGATGGCTCTTTGGGGATGTAGGGAAATTTTTTTGTCTCCGAACCACGAAATTTTTTGGAGGAATTTAGTTGAGAGATTTTATAGCGTGAGAGTGTGAAATATACACGTTTTAAACACTTTGATTATTGAGATTGGTCGTAAATTTGTTGGAGGAACTTAGTTAAGAGATTTTACAACGTGAGAGTGAAATATACATGTTGTAAGCACCTTGATTATTGAGATTGGTCGTTATTCATGGATATAGGGGAATGTCTTCTCTCCGAACAACGTAAATTTTTTGGAGGAACTTTGTTGAGAGATTTTATAGCGTGAGAGTATGAGATATAGACTTTGTAAACACTTCGATTATTGAGATTGATTGCTGTCCGTGGATGTAAGAAAATTTTTTCTTTCTGAATCACGTGAATTTTTGTCTCTTTGGTTAATTTATGTTTATGGTGTATGAGTACAACCAATCTTATTTTTGCTTCCACTACAACATGTTTGTCCATTTATAATCTAAATGTTTGGTTCTTCGTTGTAGGTTATATTCTTAGCGGGGATTGACACGTCAGCTGTGACATTAGAATGGGCGCTATCGCATTTGCTCAACAATCCAGACGTGTTAAAGAAAGCAAGAGCTGAGATAGATGATTTGGTAGGGAAAGAGCGGCTAGTGAATGAAAGTGATCTTCCAAATTTAAAGTATCTTCAAGGAATAATCTCAGAGACATTGCGGTTGAACCCAGCAGCTCCTCTTCTTGTGCCACACTGTGCATTTCAAGACTGCAAAATAGAAGGATATGATGTTCCTCGTGACACAATTGTATTGATCAATGCTTGGGCTATACATAGAGACCCAAATCTATGGGAAGATCCCACGGCGTTTAAACCAGAAAGACATGGTGACACGAGTGGAGCTGATTCTTACAAGCTATTGCCATTTGGGTTGGGGCGGAGAGCTTGTCCTGGTGTGGGAATGGCGCAACGTGTGGTTGGCTTAGCTTTAGCTTCGTTGATACAAGGCTTTGAATGGGAAAGAGTGAGCAGCTCGTTGGTTGATATGAGTGAAGGTCAAGGGCTCACTATGCCCAAAGCTCAACCTTTGGTAGCCAAGTGTAAGCCACGACCAATCATGAAAGCCATTATGAACGTAGAAATTGATAATATTTGAAATAACTTCCAAAACCAATAAAACAAATTATAATATTTGTTCTTATTGATTGATAGTGATTGATAGGCATAATGAGATGTTCTATCCTGCATGCTTTCTAGGGAATCAGATCAACATAATAAAGCCATTCACACGTTAAAGTTCAAACAGATTATTCAAAACAATTAGAATCAAAATCATAGTAGTAAAGACCATAATTCAAGCATACGAGCTAAATTGGTCAAATTTCCCTTTTACTAAGTTTAAATCTTTAATTCCTTGCTTAAAGTAGTCATATAATATGTCCATAGTCATTTTTTCCAAGTCCTAGTCGATTCCTACAAATATTTTTTCAAGTTATCCAAAAGGTTATTTATATGCTTGGAAATGTTCTGGGGTTCAACTCTCTCAGGTTTTCCGAACTTCAAATCCTAAAATGATTACATGCTTGGACATGTTCTGGGGCTCAGCTCTCTCAGGTTTTCCAAACTCCAAATCCTAAAATGAGTAAAAAAAAACAAGAACGGTTCTCGATTGAGTTGAGGTCCAAATAAACTAAAAATTATCATTGATTTAAGAAAAGTAAGATTCGACTTGACATGACTCCCAATCACTCCTAGTTGACCAGCTAAGCCAACATACAACCTCAATTGTCTATGTCTTATTCTTATCTTTAGTCATTTCATGCATAA

mRNA sequence

ATGTCTCTCCGATTCGGCTCACGCCTTGTCGTACTCATATCCTCCCCCTCAGCCGTTCAAGAATGCTTCACAAAAAACGACATCATTCTCGCAAATCGCCCATTGCTCGACACCGGAAAGTACTTAGCATACAACCACACAACCCTCGCCGTCTCACCTTACGGCGAGCACTGGCGCAACCTCCGCCGGATTTGCTCCCTCGAGATATTCTCAACCAACCGCCTCAATATGTTCGTGGGTATCCGAGAAAATGAAGTGAAACGGTTGCTGAGGAAGTTATGTGGGGATTGGTCGGATGAGTTTAAGGAAGTGGAAGTGGAGCCAATGTTGTCGGATCTTACTTTCAACGTTGTTATGGGAATGGTGGGTGGAAGGAGGTTTTATGATGAGGGGTATTCGAAGAAGTTTAGAGATTTGGTGACACAAATTATGGCGCATGGTGGGTCATCGAATCCGGGTGATTTCATACCCTTGTGGAATTGGGTTGATCCAACTGGTTTTAAGAAGAGGATAATGAATCTTGCACGAAGAGGAGATGAACTTCTTCAAGAACTAATTGATGAAGCTCGAGATGGAAAGGATGGAGGGAACACTATGATTCATCATTTACTTTGCTTGCAGAAAGATGAGCCGGAGTATTATAGTGACCTTATTATCAAAGGACTTATTCAATCATTTCATGCATAA

Coding sequence (CDS)

ATGTCTCTCCGATTCGGCTCACGCCTTGTCGTACTCATATCCTCCCCCTCAGCCGTTCAAGAATGCTTCACAAAAAACGACATCATTCTCGCAAATCGCCCATTGCTCGACACCGGAAAGTACTTAGCATACAACCACACAACCCTCGCCGTCTCACCTTACGGCGAGCACTGGCGCAACCTCCGCCGGATTTGCTCCCTCGAGATATTCTCAACCAACCGCCTCAATATGTTCGTGGGTATCCGAGAAAATGAAGTGAAACGGTTGCTGAGGAAGTTATGTGGGGATTGGTCGGATGAGTTTAAGGAAGTGGAAGTGGAGCCAATGTTGTCGGATCTTACTTTCAACGTTGTTATGGGAATGGTGGGTGGAAGGAGGTTTTATGATGAGGGGTATTCGAAGAAGTTTAGAGATTTGGTGACACAAATTATGGCGCATGGTGGGTCATCGAATCCGGGTGATTTCATACCCTTGTGGAATTGGGTTGATCCAACTGGTTTTAAGAAGAGGATAATGAATCTTGCACGAAGAGGAGATGAACTTCTTCAAGAACTAATTGATGAAGCTCGAGATGGAAAGGATGGAGGGAACACTATGATTCATCATTTACTTTGCTTGCAGAAAGATGAGCCGGAGTATTATAGTGACCTTATTATCAAAGGACTTATTCAATCATTTCATGCATAA

Protein sequence

MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQSFHA

Homology

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BLAST of Carg25150-RA vs. NCBI nr
Match: XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])

HSP 1 Score: 462.2 bits (1188), Expect = 1.0e-126
Identity = 219/224 (97.77%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SLRFGSRLVV+ISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN
Sbjct: 66  ISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 125

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWS+EF+EVE+EPMLSDLTFNVVMG
Sbjct: 126 LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTFNVVMG 185

Query: 121 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 180
           MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE
Sbjct: 186 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 245

Query: 181 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ
Sbjct: 246 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 289

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BLAST of Carg25150-RA vs. NCBI nr
Match: XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])

HSP 1 Score: 454.9 bits (1169), Expect = 1.6e-124
Identity = 214/224 (95.54%), Postives = 223/224 (99.55%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLA+SPYGEHWRN
Sbjct: 66  ISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYGEHWRN 125

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRICSLEIFST+RLN+F+GIR+NEVKRLLRKLCGDW +EF+EVEVEPMLSDLTFNVVMG
Sbjct: 126 LRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTFNVVMG 185

Query: 121 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 180
           MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE
Sbjct: 186 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 245

Query: 181 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           LLQELIDEARDGKDGGN+MIHHLLCLQKDEPEYYSDLIIKGLIQ
Sbjct: 246 LLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQ 289

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BLAST of Carg25150-RA vs. NCBI nr
Match: XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 452.2 bits (1162), Expect = 1.0e-123
Identity = 214/224 (95.54%), Postives = 221/224 (98.66%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SLRFGSRLVV+ISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLA+SPYGEHWRN
Sbjct: 66  ISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYGEHWRN 125

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCG W +E +EVE+EPMLSDLTFNVVMG
Sbjct: 126 LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGGWLEEGREVELEPMLSDLTFNVVMG 185

Query: 121 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 180
           MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE
Sbjct: 186 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 245

Query: 181 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           +LQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ
Sbjct: 246 VLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 289

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BLAST of Carg25150-RA vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 354.8 bits (909), Expect = 2.3e-94
Identity = 163/223 (73.09%), Postives = 197/223 (88.34%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGSR+VV++SSPSAVQECFTKNDI+LANRPLL +GK LAYNHTT+AV+PYGEHWRNL
Sbjct: 67  SLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +LEIFST RLNMF+GIRE+EV+ L+ KLC +  +EF  VE+EPML DLTFN+VM M
Sbjct: 127 RRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRM 186

Query: 122 VGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDEL 181
           V G+++Y+E  SKKFR++VTQ++AH G++NPGDFIP+WNW+DPTGF+KRI+ L +R DEL
Sbjct: 187 VAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDEL 246

Query: 182 LQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           LQELIDE R+ + G NTMI HLLCLQK+EPEYYSD IIKGLIQ
Sbjct: 247 LQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQ 289

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BLAST of Carg25150-RA vs. NCBI nr
Match: XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 342.0 bits (876), Expect = 1.5e-90
Identity = 159/228 (69.74%), Postives = 195/228 (85.53%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLR+GSRLVV++SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLAVSPYGEHWRNL
Sbjct: 147 SLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRNL 206

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +LEIFST+RLN+F+GIRE+EVK LLRKL GD   EF+ VE++ ML D TFN+V+ M
Sbjct: 207 RRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVRM 266

Query: 122 VGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLAR 181
           + G+++Y     DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KRIM +  
Sbjct: 267 IAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIGE 326

Query: 182 RGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           R DELLQEL+DE R+ KDGGNTMI HLL LQ  +PEY+SD IIKGL Q
Sbjct: 327 RSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQ 374

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BLAST of Carg25150-RA vs. TAIR10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 228.8 bits (582), Expect = 3.4e-60
Identity = 109/225 (48.44%), Postives = 162/225 (72.00%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLR G+RLV + SS S  +ECFTKND++LANRP     K++AY++TT+  + YG+HWRNL
Sbjct: 69  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI S+EIFS +RLN F+ IR++E++RL+ +L  ++S EF +V+++ MLSDLTFN ++ M
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188

Query: 122 VGGRRFYDEGY-----SKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLAR 181
           V G+R+Y +G      +K+ R L+  ++A  G+ N  D++P+   V  + ++ R+  LA 
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLAG 248

Query: 182 RGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKG 222
           R DE LQ L+DE R+ K+ GNTMI HLL LQ+ +P+Y++D IIKG
Sbjct: 249 RLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKG 291

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BLAST of Carg25150-RA vs. TAIR10
Match: AT4G37400.1 (cytochrome P450, family 81, subfamily F, polypeptide 3)

HSP 1 Score: 228.8 bits (582), Expect = 3.4e-60
Identity = 117/228 (51.32%), Postives = 166/228 (72.81%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFT-KNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 61
           SL+FGSR  V+ISS S   +CFT +NDIIL+NRP   T KY+AYN+TT+  +PYG+HWRN
Sbjct: 67  SLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRN 126

Query: 62  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 121
           LRRICSLEI S+NRL  F+ IR++E+ R+L +L  D +   KE+E+EP+LSDLTFN ++ 
Sbjct: 127 LRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVN---KEIELEPLLSDLTFNNIVR 186

Query: 122 MVGGRRFY-DEGYSKK----FRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 181
           MV G+R+Y DE ++++    F+ LV  I    G+ +PGD++P       + F+K++  LA
Sbjct: 187 MVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALA 246

Query: 182 RRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
              DE+LQ L++E +  KD GNTM++HLL LQ++EPEYY+D+ IKGL+
Sbjct: 247 EAMDEILQRLLEECKRDKD-GNTMVNHLLSLQQNEPEYYTDVTIKGLM 289

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BLAST of Carg25150-RA vs. TAIR10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6)

HSP 1 Score: 227.3 bits (578), Expect = 9.8e-60
Identity = 107/226 (47.35%), Postives = 158/226 (69.91%), Query Frame = 0


Query: 3   LRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNLR 62
           LR G+RL V++SS S  +ECFTKNDI+ ANRP L  GK++ YN TT+  +PYG+HWRNLR
Sbjct: 86  LRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYGDHWRNLR 145

Query: 63  RICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGMV 122
           RI +LEIF++++LN F+ +R++E+++LL +L  +    F +VE+  +L +LT N V  MV
Sbjct: 146 RIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFELTINNVFRMV 205

Query: 123 GGRRFYDEG-----YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARR 182
            G+RFY EG      +++ R L+ +I+   G+ N  D++P+  W   T F+KR+ NLA R
Sbjct: 206 AGKRFYGEGTEQDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRWF--TNFEKRVKNLAIR 265

Query: 183 GDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
            D+ LQ L+DE R  K+ G TMI HLL LQ+ +P+YY+D+ +KG+I
Sbjct: 266 IDKFLQSLVDEKRADKEKGTTMIDHLLSLQESQPDYYTDVTLKGII 309

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BLAST of Carg25150-RA vs. TAIR10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1)

HSP 1 Score: 226.5 bits (576), Expect = 1.7e-59
Identity = 119/232 (51.29%), Postives = 153/232 (65.95%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECF-TKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 61
           SLR GSRL V+ISSP+A +ECF TKNDI+LANRP    GKY+AY++T++  +PYG+HWRN
Sbjct: 68  SLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRN 127

Query: 62  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 121
           LRRI +LE+FSTNRLN    IR +EVK LL+KL     +   +VE+  +L+ LT NV+M 
Sbjct: 128 LRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIMR 187

Query: 122 MVGGRRFYDE------GYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 181
           M+ G+RF++E      G S +FR+LV +I+    + NP DF+P   W D  G  KR   +
Sbjct: 188 MMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKRI 247

Query: 182 ARRGDELLQELIDEARDGKDG---GNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
             R D LLQ  +DE R  KD     NTMI HLL  Q+ EP  YSD  IKGLI
Sbjct: 248 GERMDSLLQGFLDEHRANKDRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLI 299

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BLAST of Carg25150-RA vs. TAIR10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7)

HSP 1 Score: 224.6 bits (571), Expect = 6.4e-59
Identity = 106/227 (46.70%), Postives = 154/227 (67.84%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLR G+ L V++SS S  +ECFTKNDI+LANRP    GK++ YN TT+  +PYG+HWRNL
Sbjct: 114 SLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSAPYGDHWRNL 173

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +LEIFS+++LN F+ +R++E++ LL +L  +    F +VE+  +  DLT N ++ M
Sbjct: 174 RRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYDLTINNILRM 233

Query: 122 VGGRRFYDEG-----YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLAR 181
           V G+RFY EG      +++   L+ +I+   G  N  D+IP+  W+  T F+K +  LA 
Sbjct: 234 VAGKRFYGEGTEQDEVARRVTQLIDEIVYRAGVGNAADYIPILRWI--TDFEKGVKELAS 293

Query: 182 RGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
           R DE LQ L+DE R  K  GNTM+ HLL LQ+ +P+YY+D+ +KG+I
Sbjct: 294 RVDEFLQSLVDERRVHKQKGNTMMDHLLSLQETQPDYYTDVTLKGII 338

[Close]

BLAST of Carg25150-RA vs. Swiss-Prot
Match: sp|Q6WNQ8|C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.3e-65
Identity = 120/229 (52.40%), Postives = 163/229 (71.18%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL FGSRLVV++SS +  QECFTKNDI+LANRP   TGKY+ YN+TT+A SPYG+HWRNL
Sbjct: 68  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI S+EI S++RLN F+ IR +E+ RL++KL     + F EVE+ PM S++TFN +M M
Sbjct: 128 RRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRM 187

Query: 122 VGGRRFY-------DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 181
           V G+R+Y       D   ++ FR ++ ++++ GG++N GDF+    W D  G +KR+  +
Sbjct: 188 VSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKI 247

Query: 182 ARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
           ++R D  LQ LIDE R GK   NTMI HLL  Q+ +PEYY+D IIKGL+
Sbjct: 248 SKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLM 296

[Close]

BLAST of Carg25150-RA vs. Swiss-Prot
Match: sp|Q6WNQ9|C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 7.7e-62
Identity = 119/235 (50.64%), Postives = 165/235 (70.21%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL FGSRLVV++SSPS   ECFTKNDIILANRP   TGKY+ YN+TTL  + YG+HWRNL
Sbjct: 66  SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 125

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDE---FKEVEVEPMLSDLTFNVV 121
           RRI ++++ S NRLN F+G+R +E  RL++KL  D   E   F +VE+ P L+++TFN +
Sbjct: 126 RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 185

Query: 122 MGMVGGRRFY-DEG------YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRI 181
           M M+ G+R+Y D+G       +K+FR++++++M+  G++N GDF+PL   VD    +KR 
Sbjct: 186 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 245

Query: 182 MNLARRGDELLQELIDEARDGK--DGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
             +A+R +  L+ LI+E R G     G TMI HLL L + +PEYYSD +IKGLIQ
Sbjct: 246 KRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 300

[Close]

BLAST of Carg25150-RA vs. Swiss-Prot
Match: sp|P93147|C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 1.2e-59
Identity = 110/228 (48.25%), Postives = 158/228 (69.30%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL FGSRLVV++SS S  Q+CFTKND++LANRP   +GKY+ YN+TTL  + YGEHWRNL
Sbjct: 68  SLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +L++ S +R+N F GIR +E +RL+ +L  D S  F E+E+   L D+TFN +M M
Sbjct: 128 RRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRM 187

Query: 122 VGGRRFYDEG-------YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 181
           + G+R+Y E         + +FRD+V++++   G++N  DF+PL  ++D    +KR+ ++
Sbjct: 188 ISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDI 247

Query: 182 ARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGL 223
           + + D  L+ LI+E R  K+  NTMI HLL LQ  +PEYY+D IIKGL
Sbjct: 248 SGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGL 295

[Close]

BLAST of Carg25150-RA vs. Swiss-Prot
Match: sp|Q0WTF4|C81F3_ARATH (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 6.1e-59
Identity = 117/228 (51.32%), Postives = 166/228 (72.81%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFT-KNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 61
           SL+FGSR  V+ISS S   +CFT +NDIIL+NRP   T KY+AYN+TT+  +PYG+HWRN
Sbjct: 67  SLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRN 126

Query: 62  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 121
           LRRICSLEI S+NRL  F+ IR++E+ R+L +L  D +   KE+E+EP+LSDLTFN ++ 
Sbjct: 127 LRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVN---KEIELEPLLSDLTFNNIVR 186

Query: 122 MVGGRRFY-DEGYSKK----FRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 181
           MV G+R+Y DE ++++    F+ LV  I    G+ +PGD++P       + F+K++  LA
Sbjct: 187 MVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALA 246

Query: 182 RRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
              DE+LQ L++E +  KD GNTM++HLL LQ++EPEYY+D+ IKGL+
Sbjct: 247 EAMDEILQRLLEECKRDKD-GNTMVNHLLSLQQNEPEYYTDVTIKGLM 289

[Close]

BLAST of Carg25150-RA vs. Swiss-Prot
Match: sp|Q6WNR0|C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 8.0e-59
Identity = 109/229 (47.60%), Postives = 156/229 (68.12%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SL FGSRLVV++SS S  QECFTKND++LANRP   +GKY+ YN+TTL  + YGEHWRN
Sbjct: 67  ISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 126

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRI SL++ S +R+N F  IR +E +RL++KL  D S +F EVE+     D+TFN +M 
Sbjct: 127 LRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMR 186

Query: 121 MVGGRRFYDEG-------YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 180
           M+ G+R+Y +         + +FRD+V++++   G++N  DF+PL  ++D    +KR+  
Sbjct: 187 MISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKR 246

Query: 181 LARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGL 223
           +  + D  L  L+ E R  K+  NTMI HLL +Q+ +PEYY+D IIKGL
Sbjct: 247 IGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGL 295

[Close]

BLAST of Carg25150-RA vs. TrEMBL
Match: tr|A0A0A0LQI5|A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.3e-90
Identity = 165/236 (69.92%), Postives = 195/236 (82.63%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL+FGSRLV+L+SSPSAVQECFTKNDIILANRP L++GKYL+YN+TT+AVSPYGEHWRNL
Sbjct: 68  SLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDW--SDEFKEVEVEPMLSDLTFNVVM 121
           RRIC+LEIFST RLN F  IRE EVKRLL KLCG++   DEF+ VE+E ML DLT N+VM
Sbjct: 128 RRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLDLTSNIVM 187

Query: 122 GMVGGRRF-----------YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFK 181
            MVGG++F            DEGY K+ ++LVTQIMAH GS+NPGDFIPLWNW+DP+G+ 
Sbjct: 188 RMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGYN 247

Query: 182 KRIMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           KRIM + RR DE+LQ L+DE R+ +D GNTMI HLL LQK +P+YYSDLIIKGLIQ
Sbjct: 248 KRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQ 303

[Close]

BLAST of Carg25150-RA vs. TrEMBL
Match: tr|A0A1S3C323|A0A1S3C323_CUCME (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 6.1e-88
Identity = 156/227 (68.72%), Postives = 186/227 (81.94%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGSRLVV++SSPSAVQECFTKNDIILANRPLL++GKYL YNH+++ +SPYGE WRNL
Sbjct: 67  SLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYGEQWRNL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDW----SDEFKEVEVEPMLSDLTFNV 121
           RRI SLE+FST RLNMF+GIRE+EVKRLLRKLCG+      DEF+ VE+ P+L DLT N+
Sbjct: 127 RRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTLNI 186

Query: 122 VMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARR 181
           VM MV G+ FY+E  S KFR++VTQIM H G+SNPGDFIPLWNW+DPTGF K++  L + 
Sbjct: 187 VMRMVSGKMFYEED-SNKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKT 246

Query: 182 GDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
            DE LQ+LID  R+  DGGNTMIHHLL LQ  EPE+Y+D   K +IQ
Sbjct: 247 SDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQ 292

[Close]

BLAST of Carg25150-RA vs. TrEMBL
Match: tr|A0A1S3C1S2|A0A1S3C1S2_CUCME (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.0e-87
Identity = 161/234 (68.80%), Postives = 192/234 (82.05%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL+FGSRLVVL+SSPSA+QECFTKNDIILANRP L++GKYLAYN+TT+ VS YG+HWRNL
Sbjct: 68  SLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDW--SDEFKEVEVEPMLSDLTFNVVM 121
           RRI +LEIFST RLN F   RE EVKRLLRKL G++   DEF+ VE+EPML DLTFN+VM
Sbjct: 128 RRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFNIVM 187

Query: 122 GMVGGRRF---------YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 181
            MVGG++F          DEGY K+F++LVTQIMAHGGS+NPGDFIPL NW+DP+G+ K 
Sbjct: 188 RMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYTKN 247

Query: 182 IMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           IM + R+ DE+LQ L+DE R+ +D GNTMI HLL LQK +PEYYSD IIKGL+Q
Sbjct: 248 IMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 301

[Close]

BLAST of Carg25150-RA vs. TrEMBL
Match: tr|A0A0A0LNA4|A0A0A0LNA4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.9e-82
Identity = 154/226 (68.14%), Postives = 187/226 (82.74%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGSRLVV+ISSPS VQECFTKNDIILANRPLLDTGK+LAYNHTT+AV+PYGEHWRNL
Sbjct: 67  SLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPYGEHWRNL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDE-FKEVEVEPMLSDLTFNVVMG 121
           RRI +LE+FST+R+N+F  +RE EVK L+ +LCG  S E F+ VE+E ML DL +NV+MG
Sbjct: 127 RRIGALELFSTSRINLFSRMREEEVKALMVRLCGSSSLEGFRAVEMESMLLDLMYNVIMG 186

Query: 122 MVGGRR--FYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRG 181
           M+GG++    DEG SK+FR++VT+IMA GG+SNPGDFI +WNW+DP+G KK+I+ L +  
Sbjct: 187 MMGGKKGCEEDEGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKKKILKLGQTM 246

Query: 182 DELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           D LLQEL+D  R+    GNTMIH LL LQK EPE +SD IIKGLIQ
Sbjct: 247 DVLLQELVDGMRNESGEGNTMIHRLLQLQKIEPENHSDQIIKGLIQ 292

[Close]

BLAST of Carg25150-RA vs. TrEMBL
Match: tr|A0A0A0LTD0|A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-78
Identity = 146/231 (63.20%), Postives = 181/231 (78.35%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGS LVV+ISSPSAV+ECFTKNDIILANRP L+TGK+L+YN T LAV+PYGE WR L
Sbjct: 67  SLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWRRL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDE----FKEVEVEPMLSDLTFNV 121
           RRI + EIFST++LN F  IR++EVKRLL KLC   S E    F  VE+EP+L D T N+
Sbjct: 127 RRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTSNI 186

Query: 122 VMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIM 181
           VM MVGG+R++     DEG ++KFRD+V ++M + G++NPGDFIPLWNW+DPTG +K+IM
Sbjct: 187 VMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKIM 246

Query: 182 NLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
            +    DE+ Q LIDE R+ ++ GNTMIHHLL LQ  +PEY+SD IIKGLI
Sbjct: 247 KVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLI 297

The following BLAST results are available for this feature:

BLAST of Carg25150-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022947035.1	1.0e-126	97.77	cytochrome P450 81F3 [Cucurbita moschata]	[more]
XP_023007415.1	1.6e-124	95.54	cytochrome P450 81F3-like [Cucurbita maxima]	[more]
XP_023532689.1	1.0e-123	95.54	cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690....	[more]
XP_022149455.1	2.3e-94	73.09	cytochrome P450 81E8-like [Momordica charantia]	[more]
XP_022149460.1	1.5e-90	69.74	isoflavone 2'-hydroxylase-like [Momordica charantia]	[more]

BLAST of Carg25150-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37370.1	3.4e-60	48.44	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37400.1	3.4e-60	51.32	cytochrome P450, family 81, subfamily F, polypeptide 3	[more]
AT2G23220.1	9.8e-60	47.35	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]
AT4G37310.1	1.7e-59	51.29	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT2G23190.1	6.4e-59	46.70	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]

BLAST of Carg25150-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q6WNQ8\|C81E8_MEDTR	3.3e-65	52.40	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
sp\|Q6WNQ9\|C81E9_MEDTR	7.7e-62	50.64	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
sp\|P93147\|C81E1_GLYEC	1.2e-59	48.25	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
sp\|Q0WTF4\|C81F3_ARATH	6.1e-59	51.32	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]
sp\|Q6WNR0\|C81E7_MEDTR	8.0e-59	47.60	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Carg25150-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LQI5\|A0A0A0LQI5_CUCSA	1.3e-90	69.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1	[more]
tr\|A0A1S3C323\|A0A1S3C323_CUCME	6.1e-88	68.72	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1	[more]
tr\|A0A1S3C1S2\|A0A1S3C1S2_CUCME	1.0e-87	68.80	LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L...	[more]
tr\|A0A0A0LNA4\|A0A0A0LNA4_CUCSA	2.9e-82	68.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1	[more]
tr\|A0A0A0LTD0\|A0A0A0LTD0_CUCSA	2.0e-78	63.20	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0020037	heme binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR036396	Cyt_P450_sf
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg25150	Carg25150	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg25150-RA.exon.1	Carg25150-RA.exon.1	exon
Carg25150-RA.exon.2	Carg25150-RA.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg25150-RA.CDS.1	Carg25150-RA.CDS.1	CDS
Carg25150-RA.CDS.2	Carg25150-RA.CDS.2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg25150-RA	Carg25150-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..209 e-value: 1.2E-15 score: 57.2
IPR036396	Cytochrome P450 superfamily	GENE3D	G3DSA:1.10.630.10		coord: 1..227 e-value: 3.2E-39 score: 136.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	SSF48264	Cytochrome P450	coord: 2..226
None	No IPR available	PANTHER	PTHR24298:SF318	SUBFAMILY NOT NAMED	coord: 1..224
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..224