Carg25150 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25150
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cytochrome P450, putative
Location	Cucurbita_argyrosperma_scaffold_200 : 162507 .. 165434 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTCCGATTCGGCTCACGCCTTGTCGTACTCATATCCTCCCCCTCAGCCGTTCAAGAATGCTTCACAAAAAACGACATCATTCTCGCAAATCGCCCATTGCTCGACACCGGAAAGTACTTAGCATACAACCACACAACCCTCGCCGTCTCACCTTACGGCGAGCACTGGCGCAACCTCCGCCGGATTTGCTCCCTCGAGATATTCTCAACCAACCGCCTCAATATGTTCGTGGGTATCCGAGAAAATGAAGTGAAACGGTTGCTGAGGAAGTTATGTGGGGATTGGTCGGATGAGTTTAAGGAAGTGGAAGTGGAGCCAATGTTGTCGGATCTTACTTTCAACGTTGTTATGGGAATGGTGGGTGGAAGGAGGTTTTATGATGAGGGGTATTCGAAGAAGTTTAGAGATTTGGTGACACAAATTATGGCGCATGGTGGGTCATCGAATCCGGGTGATTTCATACCCTTGTGGAATTGGGTTGATCCAACTGGTTTTAAGAAGAGGATAATGAATCTTGCACGAAGAGGAGATGAACTTCTTCAAGAACTAATTGATGAAGCTCGAGATGGAAAGGATGGAGGGAACACTATGATTCATCATTTACTTTGCTTGCAGAAAGATGAGCCGGAGTATTATAGTGACCTTATTATCAAAGGACTTATTCAAGTACTTCTCTCTCCCTCTCTACTCTTTTCTTTATGTTTTTTTTTTCTTTTTAAGTTCACAAGTTCATGAATGTTGTGGGCATATATTGACATTTATTTGATTCATAACTCTGATTTAGTTCATAACCCTCTCTCATTTTGATTCATGAATCATCTGTGCCTATATAAAGAGATGTATTGAGTTTGATTGAGTTTGAATGTTATTTTGTATTTGTTGAGTGACATTTGGGAGGAACTTTGTTGAGAGATAGTGTGAGCGTGTGAGATATACACTTCGTAAATATTTTGATTATTGAGATTGGTTGCTATCCGTGGATATAAGGGAATTTCTTCGCTCTAACCTAAGTAAATTTTTTGGAGGAACTTTGTTAAGAGATTTTGTAGTGTGAGAGTGTGAAATATAGACTTTATAAACACTTTCATTATTGAGAATGATGGCTCTTTGGGGATGTAGGGAAATTTTTTTGTCTCCGAACCACGAAATTTTTTGGAGGAATTTAGTTGAGAGATTTTATAGCGTGAGAGTGTGAAATATACACGTTTTAAACACTTTGATTATTGAGATTGGTCGTAAATTTGTTGGAGGAACTTAGTTAAGAGATTTTACAACGTGAGAGTGAAATATACATGTTGTAAGCACCTTGATTATTGAGATTGGTCGTTATTCATGGATATAGGGGAATGTCTTCTCTCCGAACAACGTAAATTTTTTGGAGGAACTTTGTTGAGAGATTTTATAGCGTGAGAGTATGAGATATAGACTTTGTAAACACTTCGATTATTGAGATTGATTGCTGTCCGTGGATGTAAGAAAATTTTTTCTTTCTGAATCACGTGAATTTTTGTCTCTTTGGTTAATTTATGTTTATGGTGTATGAGTACAACCAATCTTATTTTTGCTTCCACTACAACATGTTTGTCCATTTATAATCTAAATGTTTGGTTCTTCGTTGTAGGTTATATTCTTAGCGGGGATTGACACGTCAGCTGTGACATTAGAATGGGCGCTATCGCATTTGCTCAACAATCCAGACGTGTTAAAGAAAGCAAGAGCTGAGATAGATGATTTGGTAGGGAAAGAGCGGCTAGTGAATGAAAGTGATCTTCCAAATTTAAAGTATCTTCAAGGAATAATCTCAGAGACATTGCGGTTGAACCCAGCAGCTCCTCTTCTTGTGCCACACTGTGCATTTCAAGACTGCAAAATAGAAGGATATGATGTTCCTCGTGACACAATTGTATTGATCAATGCTTGGGCTATACATAGAGACCCAAATCTATGGGAAGATCCCACGGCGTTTAAACCAGAAAGACATGGTGACACGAGTGGAGCTGATTCTTACAAGCTATTGCCATTTGGGTTGGGGCGGAGAGCTTGTCCTGGTGTGGGAATGGCGCAACGTGTGGTTGGCTTAGCTTTAGCTTCGTTGATACAAGGCTTTGAATGGGAAAGAGTGAGCAGCTCGTTGGTTGATATGAGTGAAGGTCAAGGGCTCACTATGCCCAAAGCTCAACCTTTGGTAGCCAAGTGTAAGCCACGACCAATCATGAAAGCCATTATGAACGTAGAAATTGATAATATTTGAAATAACTTCCAAAACCAATAAAACAAATTATAATATTTGTTCTTATTGATTGATAGTGATTGATAGGCATAATGAGATGTTCTATCCTGCATGCTTTCTAGGGAATCAGATCAACATAATAAAGCCATTCACACGTTAAAGTTCAAACAGATTATTCAAAACAATTAGAATCAAAATCATAGTAGTAAAGACCATAATTCAAGCATACGAGCTAAATTGGTCAAATTTCCCTTTTACTAAGTTTAAATCTTTAATTCCTTGCTTAAAGTAGTCATATAATATGTCCATAGTCATTTTTTCCAAGTCCTAGTCGATTCCTACAAATATTTTTTCAAGTTATCCAAAAGGTTATTTATATGCTTGGAAATGTTCTGGGGTTCAACTCTCTCAGGTTTTCCGAACTTCAAATCCTAAAATGATTACATGCTTGGACATGTTCTGGGGCTCAGCTCTCTCAGGTTTTCCAAACTCCAAATCCTAAAATGAGTAAAAAAAAACAAGAACGGTTCTCGATTGAGTTGAGGTCCAAATAAACTAAAAATTATCATTGATTTAAGAAAAGTAAGATTCGACTTGACATGACTCCCAATCACTCCTAGTTGACCAGCTAAGCCAACATACAACCTCAATTGTCTATGTCTTATTCTTATCTTTAGTCATTTCATGCATAA

mRNA sequence

ATGTCTCTCCGATTCGGCTCACGCCTTGTCGTACTCATATCCTCCCCCTCAGCCGTTCAAGAATGCTTCACAAAAAACGACATCATTCTCGCAAATCGCCCATTGCTCGACACCGGAAAGTACTTAGCATACAACCACACAACCCTCGCCGTCTCACCTTACGGCGAGCACTGGCGCAACCTCCGCCGGATTTGCTCCCTCGAGATATTCTCAACCAACCGCCTCAATATGTTCGTGGGTATCCGAGAAAATGAAGTGAAACGGTTGCTGAGGAAGTTATGTGGGGATTGGTCGGATGAGTTTAAGGAAGTGGAAGTGGAGCCAATGTTGTCGGATCTTACTTTCAACGTTGTTATGGGAATGGTGGGTGGAAGGAGGTTTTATGATGAGGGGTATTCGAAGAAGTTTAGAGATTTGGTGACACAAATTATGGCGCATGGTGGGTCATCGAATCCGGGTGATTTCATACCCTTGTGGAATTGGGTTGATCCAACTGGTTTTAAGAAGAGGATAATGAATCTTGCACGAAGAGGAGATGAACTTCTTCAAGAACTAATTGATGAAGCTCGAGATGGAAAGGATGGAGGGAACACTATGATTCATCATTTACTTTGCTTGCAGAAAGATGAGCCGGAGTATTATAGTGACCTTATTATCAAAGGACTTATTCAATCATTTCATGCATAA

Coding sequence (CDS)

ATGTCTCTCCGATTCGGCTCACGCCTTGTCGTACTCATATCCTCCCCCTCAGCCGTTCAAGAATGCTTCACAAAAAACGACATCATTCTCGCAAATCGCCCATTGCTCGACACCGGAAAGTACTTAGCATACAACCACACAACCCTCGCCGTCTCACCTTACGGCGAGCACTGGCGCAACCTCCGCCGGATTTGCTCCCTCGAGATATTCTCAACCAACCGCCTCAATATGTTCGTGGGTATCCGAGAAAATGAAGTGAAACGGTTGCTGAGGAAGTTATGTGGGGATTGGTCGGATGAGTTTAAGGAAGTGGAAGTGGAGCCAATGTTGTCGGATCTTACTTTCAACGTTGTTATGGGAATGGTGGGTGGAAGGAGGTTTTATGATGAGGGGTATTCGAAGAAGTTTAGAGATTTGGTGACACAAATTATGGCGCATGGTGGGTCATCGAATCCGGGTGATTTCATACCCTTGTGGAATTGGGTTGATCCAACTGGTTTTAAGAAGAGGATAATGAATCTTGCACGAAGAGGAGATGAACTTCTTCAAGAACTAATTGATGAAGCTCGAGATGGAAAGGATGGAGGGAACACTATGATTCATCATTTACTTTGCTTGCAGAAAGATGAGCCGGAGTATTATAGTGACCTTATTATCAAAGGACTTATTCAATCATTTCATGCATAA

Protein sequence

MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQSFHA

Homology

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BLAST of Carg25150 vs. NCBI nr
Match: XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])

HSP 1 Score: 462.2 bits (1188), Expect = 1.0e-126
Identity = 219/224 (97.77%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SLRFGSRLVV+ISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN
Sbjct: 66  ISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 125

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWS+EF+EVE+EPMLSDLTFNVVMG
Sbjct: 126 LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTFNVVMG 185

Query: 121 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 180
           MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE
Sbjct: 186 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 245

Query: 181 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ
Sbjct: 246 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 289

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BLAST of Carg25150 vs. NCBI nr
Match: XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])

HSP 1 Score: 454.9 bits (1169), Expect = 1.6e-124
Identity = 214/224 (95.54%), Postives = 223/224 (99.55%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLA+SPYGEHWRN
Sbjct: 66  ISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYGEHWRN 125

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRICSLEIFST+RLN+F+GIR+NEVKRLLRKLCGDW +EF+EVEVEPMLSDLTFNVVMG
Sbjct: 126 LRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTFNVVMG 185

Query: 121 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 180
           MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE
Sbjct: 186 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 245

Query: 181 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           LLQELIDEARDGKDGGN+MIHHLLCLQKDEPEYYSDLIIKGLIQ
Sbjct: 246 LLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQ 289

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BLAST of Carg25150 vs. NCBI nr
Match: XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 452.2 bits (1162), Expect = 1.0e-123
Identity = 214/224 (95.54%), Postives = 221/224 (98.66%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SLRFGSRLVV+ISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLA+SPYGEHWRN
Sbjct: 66  ISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYGEHWRN 125

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCG W +E +EVE+EPMLSDLTFNVVMG
Sbjct: 126 LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGGWLEEGREVELEPMLSDLTFNVVMG 185

Query: 121 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 180
           MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE
Sbjct: 186 MVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDE 245

Query: 181 LLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           +LQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ
Sbjct: 246 VLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 289

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BLAST of Carg25150 vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 354.8 bits (909), Expect = 2.3e-94
Identity = 163/223 (73.09%), Postives = 197/223 (88.34%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGSR+VV++SSPSAVQECFTKNDI+LANRPLL +GK LAYNHTT+AV+PYGEHWRNL
Sbjct: 67  SLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +LEIFST RLNMF+GIRE+EV+ L+ KLC +  +EF  VE+EPML DLTFN+VM M
Sbjct: 127 RRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRM 186

Query: 122 VGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDEL 181
           V G+++Y+E  SKKFR++VTQ++AH G++NPGDFIP+WNW+DPTGF+KRI+ L +R DEL
Sbjct: 187 VAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDEL 246

Query: 182 LQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           LQELIDE R+ + G NTMI HLLCLQK+EPEYYSD IIKGLIQ
Sbjct: 247 LQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQ 289

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BLAST of Carg25150 vs. NCBI nr
Match: XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 342.0 bits (876), Expect = 1.5e-90
Identity = 159/228 (69.74%), Postives = 195/228 (85.53%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLR+GSRLVV++SSPSAVQECFTKNDI+LANRPLL +GK+LAYNHTTLAVSPYGEHWRNL
Sbjct: 147 SLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRNL 206

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +LEIFST+RLN+F+GIRE+EVK LLRKL GD   EF+ VE++ ML D TFN+V+ M
Sbjct: 207 RRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVRM 266

Query: 122 VGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLAR 181
           + G+++Y     DEG SKKFR++V +I+A+GG++N GDF+PLWNW+DPTG++KRIM +  
Sbjct: 267 IAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIGE 326

Query: 182 RGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           R DELLQEL+DE R+ KDGGNTMI HLL LQ  +PEY+SD IIKGL Q
Sbjct: 327 RSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQ 374

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BLAST of Carg25150 vs. TAIR10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 228.8 bits (582), Expect = 3.4e-60
Identity = 109/225 (48.44%), Postives = 162/225 (72.00%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLR G+RLV + SS S  +ECFTKND++LANRP     K++AY++TT+  + YG+HWRNL
Sbjct: 69  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI S+EIFS +RLN F+ IR++E++RL+ +L  ++S EF +V+++ MLSDLTFN ++ M
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188

Query: 122 VGGRRFYDEGY-----SKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLAR 181
           V G+R+Y +G      +K+ R L+  ++A  G+ N  D++P+   V  + ++ R+  LA 
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLAG 248

Query: 182 RGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKG 222
           R DE LQ L+DE R+ K+ GNTMI HLL LQ+ +P+Y++D IIKG
Sbjct: 249 RLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKG 291

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BLAST of Carg25150 vs. TAIR10
Match: AT4G37400.1 (cytochrome P450, family 81, subfamily F, polypeptide 3)

HSP 1 Score: 228.8 bits (582), Expect = 3.4e-60
Identity = 117/228 (51.32%), Postives = 166/228 (72.81%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFT-KNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 61
           SL+FGSR  V+ISS S   +CFT +NDIIL+NRP   T KY+AYN+TT+  +PYG+HWRN
Sbjct: 67  SLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRN 126

Query: 62  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 121
           LRRICSLEI S+NRL  F+ IR++E+ R+L +L  D +   KE+E+EP+LSDLTFN ++ 
Sbjct: 127 LRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVN---KEIELEPLLSDLTFNNIVR 186

Query: 122 MVGGRRFY-DEGYSKK----FRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 181
           MV G+R+Y DE ++++    F+ LV  I    G+ +PGD++P       + F+K++  LA
Sbjct: 187 MVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALA 246

Query: 182 RRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
              DE+LQ L++E +  KD GNTM++HLL LQ++EPEYY+D+ IKGL+
Sbjct: 247 EAMDEILQRLLEECKRDKD-GNTMVNHLLSLQQNEPEYYTDVTIKGLM 289

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BLAST of Carg25150 vs. TAIR10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6)

HSP 1 Score: 227.3 bits (578), Expect = 9.8e-60
Identity = 107/226 (47.35%), Postives = 158/226 (69.91%), Query Frame = 0


Query: 3   LRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNLR 62
           LR G+RL V++SS S  +ECFTKNDI+ ANRP L  GK++ YN TT+  +PYG+HWRNLR
Sbjct: 86  LRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYGDHWRNLR 145

Query: 63  RICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGMV 122
           RI +LEIF++++LN F+ +R++E+++LL +L  +    F +VE+  +L +LT N V  MV
Sbjct: 146 RIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFELTINNVFRMV 205

Query: 123 GGRRFYDEG-----YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARR 182
            G+RFY EG      +++ R L+ +I+   G+ N  D++P+  W   T F+KR+ NLA R
Sbjct: 206 AGKRFYGEGTEQDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRWF--TNFEKRVKNLAIR 265

Query: 183 GDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
            D+ LQ L+DE R  K+ G TMI HLL LQ+ +P+YY+D+ +KG+I
Sbjct: 266 IDKFLQSLVDEKRADKEKGTTMIDHLLSLQESQPDYYTDVTLKGII 309

[Close]

BLAST of Carg25150 vs. TAIR10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1)

HSP 1 Score: 226.5 bits (576), Expect = 1.7e-59
Identity = 119/232 (51.29%), Postives = 153/232 (65.95%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECF-TKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 61
           SLR GSRL V+ISSP+A +ECF TKNDI+LANRP    GKY+AY++T++  +PYG+HWRN
Sbjct: 68  SLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRN 127

Query: 62  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 121
           LRRI +LE+FSTNRLN    IR +EVK LL+KL     +   +VE+  +L+ LT NV+M 
Sbjct: 128 LRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIMR 187

Query: 122 MVGGRRFYDE------GYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 181
           M+ G+RF++E      G S +FR+LV +I+    + NP DF+P   W D  G  KR   +
Sbjct: 188 MMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKRI 247

Query: 182 ARRGDELLQELIDEARDGKDG---GNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
             R D LLQ  +DE R  KD     NTMI HLL  Q+ EP  YSD  IKGLI
Sbjct: 248 GERMDSLLQGFLDEHRANKDRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLI 299

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BLAST of Carg25150 vs. TAIR10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7)

HSP 1 Score: 224.6 bits (571), Expect = 6.4e-59
Identity = 106/227 (46.70%), Postives = 154/227 (67.84%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLR G+ L V++SS S  +ECFTKNDI+LANRP    GK++ YN TT+  +PYG+HWRNL
Sbjct: 114 SLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSAPYGDHWRNL 173

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +LEIFS+++LN F+ +R++E++ LL +L  +    F +VE+  +  DLT N ++ M
Sbjct: 174 RRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYDLTINNILRM 233

Query: 122 VGGRRFYDEG-----YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLAR 181
           V G+RFY EG      +++   L+ +I+   G  N  D+IP+  W+  T F+K +  LA 
Sbjct: 234 VAGKRFYGEGTEQDEVARRVTQLIDEIVYRAGVGNAADYIPILRWI--TDFEKGVKELAS 293

Query: 182 RGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
           R DE LQ L+DE R  K  GNTM+ HLL LQ+ +P+YY+D+ +KG+I
Sbjct: 294 RVDEFLQSLVDERRVHKQKGNTMMDHLLSLQETQPDYYTDVTLKGII 338

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BLAST of Carg25150 vs. Swiss-Prot
Match: sp|Q6WNQ8|C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.3e-65
Identity = 120/229 (52.40%), Postives = 163/229 (71.18%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL FGSRLVV++SS +  QECFTKNDI+LANRP   TGKY+ YN+TT+A SPYG+HWRNL
Sbjct: 68  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI S+EI S++RLN F+ IR +E+ RL++KL     + F EVE+ PM S++TFN +M M
Sbjct: 128 RRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRM 187

Query: 122 VGGRRFY-------DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 181
           V G+R+Y       D   ++ FR ++ ++++ GG++N GDF+    W D  G +KR+  +
Sbjct: 188 VSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKI 247

Query: 182 ARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
           ++R D  LQ LIDE R GK   NTMI HLL  Q+ +PEYY+D IIKGL+
Sbjct: 248 SKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLM 296

[Close]

BLAST of Carg25150 vs. Swiss-Prot
Match: sp|Q6WNQ9|C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 7.7e-62
Identity = 119/235 (50.64%), Postives = 165/235 (70.21%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL FGSRLVV++SSPS   ECFTKNDIILANRP   TGKY+ YN+TTL  + YG+HWRNL
Sbjct: 66  SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 125

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDE---FKEVEVEPMLSDLTFNVV 121
           RRI ++++ S NRLN F+G+R +E  RL++KL  D   E   F +VE+ P L+++TFN +
Sbjct: 126 RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 185

Query: 122 MGMVGGRRFY-DEG------YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRI 181
           M M+ G+R+Y D+G       +K+FR++++++M+  G++N GDF+PL   VD    +KR 
Sbjct: 186 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 245

Query: 182 MNLARRGDELLQELIDEARDGK--DGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
             +A+R +  L+ LI+E R G     G TMI HLL L + +PEYYSD +IKGLIQ
Sbjct: 246 KRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 300

[Close]

BLAST of Carg25150 vs. Swiss-Prot
Match: sp|P93147|C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 1.2e-59
Identity = 110/228 (48.25%), Postives = 158/228 (69.30%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL FGSRLVV++SS S  Q+CFTKND++LANRP   +GKY+ YN+TTL  + YGEHWRNL
Sbjct: 68  SLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMGM 121
           RRI +L++ S +R+N F GIR +E +RL+ +L  D S  F E+E+   L D+TFN +M M
Sbjct: 128 RRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRM 187

Query: 122 VGGRRFYDEG-------YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 181
           + G+R+Y E         + +FRD+V++++   G++N  DF+PL  ++D    +KR+ ++
Sbjct: 188 ISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDI 247

Query: 182 ARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGL 223
           + + D  L+ LI+E R  K+  NTMI HLL LQ  +PEYY+D IIKGL
Sbjct: 248 SGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGL 295

[Close]

BLAST of Carg25150 vs. Swiss-Prot
Match: sp|Q0WTF4|C81F3_ARATH (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 6.1e-59
Identity = 117/228 (51.32%), Postives = 166/228 (72.81%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFT-KNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 61
           SL+FGSR  V+ISS S   +CFT +NDIIL+NRP   T KY+AYN+TT+  +PYG+HWRN
Sbjct: 67  SLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRN 126

Query: 62  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 121
           LRRICSLEI S+NRL  F+ IR++E+ R+L +L  D +   KE+E+EP+LSDLTFN ++ 
Sbjct: 127 LRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVN---KEIELEPLLSDLTFNNIVR 186

Query: 122 MVGGRRFY-DEGYSKK----FRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLA 181
           MV G+R+Y DE ++++    F+ LV  I    G+ +PGD++P       + F+K++  LA
Sbjct: 187 MVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALA 246

Query: 182 RRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
              DE+LQ L++E +  KD GNTM++HLL LQ++EPEYY+D+ IKGL+
Sbjct: 247 EAMDEILQRLLEECKRDKD-GNTMVNHLLSLQQNEPEYYTDVTIKGLM 289

[Close]

BLAST of Carg25150 vs. Swiss-Prot
Match: sp|Q6WNR0|C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 8.0e-59
Identity = 109/229 (47.60%), Postives = 156/229 (68.12%), Query Frame = 0


Query: 1   MSLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRN 60
           +SL FGSRLVV++SS S  QECFTKND++LANRP   +GKY+ YN+TTL  + YGEHWRN
Sbjct: 67  ISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 126

Query: 61  LRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDEFKEVEVEPMLSDLTFNVVMG 120
           LRRI SL++ S +R+N F  IR +E +RL++KL  D S +F EVE+     D+TFN +M 
Sbjct: 127 LRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMR 186

Query: 121 MVGGRRFYDEG-------YSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 180
           M+ G+R+Y +         + +FRD+V++++   G++N  DF+PL  ++D    +KR+  
Sbjct: 187 MISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKR 246

Query: 181 LARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGL 223
           +  + D  L  L+ E R  K+  NTMI HLL +Q+ +PEYY+D IIKGL
Sbjct: 247 IGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGL 295

[Close]

BLAST of Carg25150 vs. TrEMBL
Match: tr|A0A0A0LQI5|A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.3e-90
Identity = 165/236 (69.92%), Postives = 195/236 (82.63%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL+FGSRLV+L+SSPSAVQECFTKNDIILANRP L++GKYL+YN+TT+AVSPYGEHWRNL
Sbjct: 68  SLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDW--SDEFKEVEVEPMLSDLTFNVVM 121
           RRIC+LEIFST RLN F  IRE EVKRLL KLCG++   DEF+ VE+E ML DLT N+VM
Sbjct: 128 RRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLDLTSNIVM 187

Query: 122 GMVGGRRF-----------YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFK 181
            MVGG++F            DEGY K+ ++LVTQIMAH GS+NPGDFIPLWNW+DP+G+ 
Sbjct: 188 RMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGYN 247

Query: 182 KRIMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           KRIM + RR DE+LQ L+DE R+ +D GNTMI HLL LQK +P+YYSDLIIKGLIQ
Sbjct: 248 KRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQ 303

[Close]

BLAST of Carg25150 vs. TrEMBL
Match: tr|A0A1S3C323|A0A1S3C323_CUCME (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 6.1e-88
Identity = 156/227 (68.72%), Postives = 186/227 (81.94%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGSRLVV++SSPSAVQECFTKNDIILANRPLL++GKYL YNH+++ +SPYGE WRNL
Sbjct: 67  SLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYGEQWRNL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDW----SDEFKEVEVEPMLSDLTFNV 121
           RRI SLE+FST RLNMF+GIRE+EVKRLLRKLCG+      DEF+ VE+ P+L DLT N+
Sbjct: 127 RRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTLNI 186

Query: 122 VMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARR 181
           VM MV G+ FY+E  S KFR++VTQIM H G+SNPGDFIPLWNW+DPTGF K++  L + 
Sbjct: 187 VMRMVSGKMFYEED-SNKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKT 246

Query: 182 GDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
            DE LQ+LID  R+  DGGNTMIHHLL LQ  EPE+Y+D   K +IQ
Sbjct: 247 SDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQ 292

[Close]

BLAST of Carg25150 vs. TrEMBL
Match: tr|A0A1S3C1S2|A0A1S3C1S2_CUCME (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.0e-87
Identity = 161/234 (68.80%), Postives = 192/234 (82.05%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SL+FGSRLVVL+SSPSA+QECFTKNDIILANRP L++GKYLAYN+TT+ VS YG+HWRNL
Sbjct: 68  SLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRNL 127

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDW--SDEFKEVEVEPMLSDLTFNVVM 121
           RRI +LEIFST RLN F   RE EVKRLLRKL G++   DEF+ VE+EPML DLTFN+VM
Sbjct: 128 RRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFNIVM 187

Query: 122 GMVGGRRF---------YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKR 181
            MVGG++F          DEGY K+F++LVTQIMAHGGS+NPGDFIPL NW+DP+G+ K 
Sbjct: 188 RMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYTKN 247

Query: 182 IMNLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           IM + R+ DE+LQ L+DE R+ +D GNTMI HLL LQK +PEYYSD IIKGL+Q
Sbjct: 248 IMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 301

[Close]

BLAST of Carg25150 vs. TrEMBL
Match: tr|A0A0A0LNA4|A0A0A0LNA4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.9e-82
Identity = 154/226 (68.14%), Postives = 187/226 (82.74%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGSRLVV+ISSPS VQECFTKNDIILANRPLLDTGK+LAYNHTT+AV+PYGEHWRNL
Sbjct: 67  SLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPYGEHWRNL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDE-FKEVEVEPMLSDLTFNVVMG 121
           RRI +LE+FST+R+N+F  +RE EVK L+ +LCG  S E F+ VE+E ML DL +NV+MG
Sbjct: 127 RRIGALELFSTSRINLFSRMREEEVKALMVRLCGSSSLEGFRAVEMESMLLDLMYNVIMG 186

Query: 122 MVGGRR--FYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRG 181
           M+GG++    DEG SK+FR++VT+IMA GG+SNPGDFI +WNW+DP+G KK+I+ L +  
Sbjct: 187 MMGGKKGCEEDEGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKKKILKLGQTM 246

Query: 182 DELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQ 225
           D LLQEL+D  R+    GNTMIH LL LQK EPE +SD IIKGLIQ
Sbjct: 247 DVLLQELVDGMRNESGEGNTMIHRLLQLQKIEPENHSDQIIKGLIQ 292

[Close]

BLAST of Carg25150 vs. TrEMBL
Match: tr|A0A0A0LTD0|A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-78
Identity = 146/231 (63.20%), Postives = 181/231 (78.35%), Query Frame = 0


Query: 2   SLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNL 61
           SLRFGS LVV+ISSPSAV+ECFTKNDIILANRP L+TGK+L+YN T LAV+PYGE WR L
Sbjct: 67  SLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYGELWRRL 126

Query: 62  RRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSDE----FKEVEVEPMLSDLTFNV 121
           RRI + EIFST++LN F  IR++EVKRLL KLC   S E    F  VE+EP+L D T N+
Sbjct: 127 RRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTSNI 186

Query: 122 VMGMVGGRRFY-----DEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIM 181
           VM MVGG+R++     DEG ++KFRD+V ++M + G++NPGDFIPLWNW+DPTG +K+IM
Sbjct: 187 VMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKIM 246

Query: 182 NLARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLI 224
            +    DE+ Q LIDE R+ ++ GNTMIHHLL LQ  +PEY+SD IIKGLI
Sbjct: 247 KVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLI 297

The following BLAST results are available for this feature:

BLAST of Carg25150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022947035.1	1.0e-126	97.77	cytochrome P450 81F3 [Cucurbita moschata]	[more]
XP_023007415.1	1.6e-124	95.54	cytochrome P450 81F3-like [Cucurbita maxima]	[more]
XP_023532689.1	1.0e-123	95.54	cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690....	[more]
XP_022149455.1	2.3e-94	73.09	cytochrome P450 81E8-like [Momordica charantia]	[more]
XP_022149460.1	1.5e-90	69.74	isoflavone 2'-hydroxylase-like [Momordica charantia]	[more]

BLAST of Carg25150 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37370.1	3.4e-60	48.44	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37400.1	3.4e-60	51.32	cytochrome P450, family 81, subfamily F, polypeptide 3	[more]
AT2G23220.1	9.8e-60	47.35	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]
AT4G37310.1	1.7e-59	51.29	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT2G23190.1	6.4e-59	46.70	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]

BLAST of Carg25150 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q6WNQ8\|C81E8_MEDTR	3.3e-65	52.40	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
sp\|Q6WNQ9\|C81E9_MEDTR	7.7e-62	50.64	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
sp\|P93147\|C81E1_GLYEC	1.2e-59	48.25	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
sp\|Q0WTF4\|C81F3_ARATH	6.1e-59	51.32	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]
sp\|Q6WNR0\|C81E7_MEDTR	8.0e-59	47.60	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Carg25150 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LQI5\|A0A0A0LQI5_CUCSA	1.3e-90	69.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1	[more]
tr\|A0A1S3C323\|A0A1S3C323_CUCME	6.1e-88	68.72	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1	[more]
tr\|A0A1S3C1S2\|A0A1S3C1S2_CUCME	1.0e-87	68.80	LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L...	[more]
tr\|A0A0A0LNA4\|A0A0A0LNA4_CUCSA	2.9e-82	68.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1	[more]
tr\|A0A0A0LTD0\|A0A0A0LTD0_CUCSA	2.0e-78	63.20	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0020037	heme binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR036396	Cyt_P450_sf
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25150-RA	Carg25150-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..209 e-value: 1.2E-15 score: 57.2
IPR036396	Cytochrome P450 superfamily	GENE3D	G3DSA:1.10.630.10		coord: 1..227 e-value: 3.2E-39 score: 136.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	SSF48264	Cytochrome P450	coord: 2..226
None	No IPR available	PANTHER	PTHR24298:SF318	SUBFAMILY NOT NAMED	coord: 1..224
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..224

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg25150	Csa2G423650	Cucumber (Chinese Long) v2	carcuB0442
Carg25150	MELO3C019221	Melon (DHL92) v3.5.1	carmeB0416
Carg25150	CSPI02G26790	Wild cucumber (PI 183967)	carcpiB0447
Carg25150	CmoCh20G009940	Cucurbita moschata (Rifu)	carcmoB0555
Carg25150	Lsi10G001660	Bottle gourd (USVL1VR-Ls)	carlsiB374
Carg25150	Cp4.1LG05g11670	Cucurbita pepo (Zucchini)	carcpeB0577
Carg25150	Cp4.1LG16g01420	Cucurbita pepo (Zucchini)	carcpeB0576
Carg25150	MELO3C019210.2	Melon (DHL92) v3.6.1	carmedB0420
Carg25150	Cla97C02G034880	Watermelon (97103) v2	carwmbB0436
Carg25150	Bhi10G001162	Wax gourd	carwgoB0497

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg25150	Cucurbita maxima (Rimu)	carcmaB0572
Carg25150	Cucurbita maxima (Rimu)	carcmaB0573
Carg25150	Cucurbita moschata (Rifu)	carcmoB0556