Cp4.1LG05g08730 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG05g08730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionSubtilisin-like protease
LocationCp4.1LG05 : 5735179 .. 5738761 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTATAGCTACAAACGTAGCTTTTCTGGGTTCTCCGCGAAGCTTAATGCAAGCCAAGCAATGACGTTATCGAGTAATTAATGGATCGAATTTGGTTAAGAATTCGATGAAATTTAAGTTAATATGAAATGGGTATTAGTTTATGAGTTTGATATTGAAAGGAATGCATGCAATATTGCCATGCAGAGATGGAAGGAGTGATATCCATATTCAAGAGTAAAACTATGGAGCTCCACACAACAAGAAGTTGGGACTTCTTGGGGCTTCCCATTCCTTCGTATAGCGGTAGTAGGACGTTTGATACACCACGTTCGAGTAAGCTGGCCGATGGCGACGACGTTGTTGTTGGCATCTTCGATTCAGGTCTTTGGATTAATTTGCATTATATTCGTTAATCATATGTCTCGTGTCTATGTAAGTTAAATTACAAATTTATTCTCTATGATATATGATCAAATTTGATTGTTAGGCATATGGCCTGAATCGGAAAGTTTCGAGGATGATCGGTGGATGTCCCCGGTTCCATGCAGCTGGAAGGGCAAATGCGTGAAAGCGTATAGATTCAACCCTTCATTAGCTTGCAATCGCAAGCTAATTGGCGCTCGTTACTATCTCAAAGGTTTCGAGGCCGAATACGGAGGACTCAACACAAGCGGGAACCCTGAGTTTGAATCGCCTCGAGACTTTTTGGGGCACGGAACTCATACAGCTTCAACGGCCGTGGGTGGAATTGTAGAGGACGTGAGCTTCACGGGGTCGTCACTCGGCAAAGGCATTGCTCGAGGAGGAGCGCCAAGAGCTAGGCTGGCTGTGTACAAGGTGTGCTGGGGAAAGGACTACGAAGGGAAGTGCACGGACGCAGACGTGATGGCTGCGTTTGACGACGCTTTATGCGACGGAGTCGACGTTATCTCGGCGTCGTTCGGAGGGTCGCCGCCGTTGGCTCCATTTTTTGAGTCTAGTAGTGCGATTGGGTCGTTCCATGGCATGCAGAAGGGAGTGAGTGTTGTGTTCTCAGCTGGTAACGATGGACCTTATCGTTCACTCGTTCAAAATGTGTCTCCGTGGAGCATTTGTGTTGCTGCTTCAACTATGGATCGAACCTTTCCCACTCAAATACTCATCCAAAACAGGATCTCTATTATGGTTACCTCTCTTCTCTCTCTCTCAAACAAAATATTATTATGACCTTATTTATCTTTTGGTTGATTATTATGTTCATTCTCTTCATCAGGGTGAGAGCTTGATCACTACAAACATAATTAACGCAAAACTAGCGAATGCGATCAACTATTTTATAGACGGGTGAGTTTGGATATCATTAGAGAAGCTCACGTCATTTTAAATTGAATCTCCTGTCGATGTCTTAAACAGAGATTAGACATATATAACTCAGTGTCAAAACATCCCCTCTTTAATAATAATACAATAGTTTGTTTTGATATTTTGTTTGGTTTGGTGTAAAATTCAGAGTTTGCGAGCGGCGAAGTATAAGGAAAGGTAGGAAATCGGGGGCAGGGAAAGTGGTGGTTTGTTTCTCCACGGTAGGCCCAGTTTCGATGGGGGATGCACAAGAAGCTCTGGACGCCATCAATGCCTCGGCTTTGATATTTGGTGCCCCGCCCACGCTGCAGCTCCCTGACCTTGATCTCCTCCCCACCGTTCGAATTGACATTACTCACGCAACTCAAATTAGAAACTTTCTTGCTGAACTGCCCAGGTCTCATTTACTTCTTCTTTTTCCTTCTTTCACTTATTTACCATCCATTCTAATTCTTATTTGTACTCGTGTTTGTTTCGAAGGCTGCCAGTAGTAGAGATCAGGGCTGCAAGAAGCGTGATTGGGAAGTCGGTGGCTCCGAGTGTGGCATACTTCTCGTCTAGAGGACCGAGCTCACTTTCGCCTGACATTCTTAAGGTACAAAATTTGATCCAGTTCCACAAATTTTATAGACAGTTGTGCAAATGGCAATTTGACACGAGTTTAAGAAAGTGCAAATATGACGGTCGTCTATAAAGCACGAGACCTAATGAATCTTGATTGATATTTATATGTTTGATATATCGTATCAATATGATTAACAACGGTATATCAATGATATGAGTGTTTCTTTCTTTCTTACTTTTAAGTGGTTGTTATATGATTATTTGGTCTATCGTTGATATGTCATATATTAATAAATTAAAAATAATAAGTCGGTGATAACTTGGTATCAATAACAAAAAAGTTAATAGCAAATAATAATGAGTTACTAATAACCATACACCTCTATTGTCTGGAGGCTAGCATATGTTGTCCTTTTTGGGATTTTGCTTTCGGACTTCTTTCGAAGTTTTAAAAATGCATTTACTAGAGAGAGGTTTCCACACCCTTATAAATTAATTATCCTCTTTGGACTTTCCCTTTTGAGTTTCCCCTCAAGATTTTTAAAACGCGTTTACTAGGGAGAGGTTTCCACACCCTTATAAAAGTGTCTGTTTCTCTCTTCAACCGATGTAGAATCTCACAACATCTATCACTAATAGACAGAAATAGTCCATCAACGATAAACATGAAATTTTTTTATCAAGTGATCTATCGATAATAGAAAACTATCACCGACAGACGTGTTAAATGGTGTATGATGGTCATTTTGAACACTAATGAGGGTATTATGTATGGTGCAGCCAGACATAAGTGCCCCAGGAGTGAACATATTGGCGGCATGGCCTCCAGAAACTGCTCCAACAGTGCGGCCAACATCGACAGAAGGAGAAAGCCAAAGGCAAGTCAAATGGAATTTCCAATCAGGGACTTCAATGTCATGCCCTCACATCTCTGGAGTCGTTGCCCTCATCAAATCTCTGCATCCAAGCTGGTCTCCTGCAGCCATTAGATCTGCCCTCATCACCACAGGTTAACCATTCCCGTCCTTTTCATTTTAGTCTCTAAACGTCCTTTTCATTTTAGTCTCTAAACTTTCAAATAACCAGTCTATTAACTAACTCGTGGCTCATAATTCATAATTCAGCCACCAAGAGAGACTCCACTCGCAACACCATCCTAGCAGGTGGATCCACGAAACCATCCGATCCATTCGACATTGGAGGTGGCCAAGTGAACCCATTGAAGGCAGTAAATCCAGGACTAGTGTACGACATGACAGCCAATGATTACATTATTTTTCTCTGCAATATTGGGTACACAGAGCAGCAAATCAGGATGATTGTGAACCCTTCTCCACAAACACTTGTTTGTTGTCCACCATTTCTATCCACCTCCATTGCCAATCTCAATTACCCTTCCATTACACTTGCAAACCTCAAATCCACAACCACAATCAAAAGGACTGTTCGTAATGTAGCAGAAAACAAAAATGCCATCTACTTTCTTAAGCTGAATCCTCCCAATGGAGTGCAAGTATTGGTATGGCCTCGGATTCTTCTTTTCTCTTGGTTTAGGCAGCACGTTTCTTACTACATCACCATAACCCCGCTCAAGAAAGCTCGTGGGAGGTATGATTTTGGGGAGATTGAATGGTCAGATGGGTTCCATAGGGTCACAAGTCCTCTGGTTGTTCGTGTTAGCAGCGCTAGCTAG

mRNA sequence

ATGTTGTATAGCTACAAACGTAGCTTTTCTGGGTTCTCCGCGAAGCTTAATGCAAGCCAAGCAATGACGTTATCGAAGATGGAAGGAGTGATATCCATATTCAAGAGTAAAACTATGGAGCTCCACACAACAAGAAGTTGGGACTTCTTGGGGCTTCCCATTCCTTCGTATAGCGGTAGTAGGACGTTTGATACACCACGTTCGAGTAAGCTGGCCGATGGCGACGACGTTGTTGTTGGCATCTTCGATTCAGGCATATGGCCTGAATCGGAAAGTTTCGAGGATGATCGGTGGATGTCCCCGGTTCCATGCAGCTGGAAGGGCAAATGCGTGAAAGCGTATAGATTCAACCCTTCATTAGCTTGCAATCGCAAGCTAATTGGCGCTCGTTACTATCTCAAAGGTTTCGAGGCCGAATACGGAGGACTCAACACAAGCGGGAACCCTGAGTTTGAATCGCCTCGAGACTTTTTGGGGCACGGAACTCATACAGCTTCAACGGCCGTGGGTGGAATTGTAGAGGACGTGAGCTTCACGGGGTCGTCACTCGGCAAAGGCATTGCTCGAGGAGGAGCGCCAAGAGCTAGGCTGGCTGTGTACAAGGTGTGCTGGGGAAAGGACTACGAAGGGAAGTGCACGGACGCAGACGTGATGGCTGCGTTTGACGACGCTTTATGCGACGGAGTCGACGTTATCTCGGCGTCGTTCGGAGGGTCGCCGCCGTTGGCTCCATTTTTTGAGTCTAGTAGTGCGATTGGGTCGTTCCATGGCATGCAGAAGGGAGTGAGTGTTGTGTTCTCAGCTGGTAACGATGGACCTTATCGTTCACTCGTTCAAAATGTGTCTCCGTGGAGCATTTGTGTTGCTGCTTCAACTATGGATCGAACCTTTCCCACTCAAATACTCATCCAAAACAGGATCTCTATTATGGGTGAGAGCTTGATCACTACAAACATAATTAACGCAAAACTAGCGAATGCGATCAACTATTTTATAGACGGAGTTTGCGAGCGGCGAAGTATAAGGAAAGGTAGGAAATCGGGGGCAGGGAAAGTGGTGGTTTGTTTCTCCACGGTAGGCCCAGTTTCGATGGGGGATGCACAAGAAGCTCTGGACGCCATCAATGCCTCGGCTTTGATATTTGGTGCCCCGCCCACGCTGCAGCTCCCTGACCTTGATCTCCTCCCCACCGTTCGAATTGACATTACTCACGCAACTCAAATTAGAAACTTTCTTGCTGAACTGCCCAGGCTGCCAGTAGTAGAGATCAGGGCTGCAAGAAGCGTGATTGGGAAGTCGGTGGCTCCGAGTGTGGCATACTTCTCGTCTAGAGGACCGAGCTCACTTTCGCCTGACATTCTTAAGCCAGACATAAGTGCCCCAGGAGTGAACATATTGGCGGCATGGCCTCCAGAAACTGCTCCAACAGTGCGGCCAACATCGACAGAAGGAGAAAGCCAAAGGCAAGTCAAATGGAATTTCCAATCAGGGACTTCAATGTCATGCCCTCACATCTCTGGAGTCGTTGCCCTCATCAAATCTCTGCATCCAAGCTGGTCTCCTGCAGCCATTAGATCTGCCCTCATCACCACAGCCACCAAGAGAGACTCCACTCGCAACACCATCCTAGCAGGTGGATCCACGAAACCATCCGATCCATTCGACATTGGAGGTGGCCAAGTGAACCCATTGAAGGCAGTAAATCCAGGACTAGTGTACGACATGACAGCCAATGATTACATTATTTTTCTCTGCAATATTGGGTACACAGAGCAGCAAATCAGGATGATTGTGAACCCTTCTCCACAAACACTTGTTTGTTGTCCACCATTTCTATCCACCTCCATTGCCAATCTCAATTACCCTTCCATTACACTTGCAAACCTCAAATCCACAACCACAATCAAAAGGACTGTTCGTAATGTAGCAGAAAACAAAAATGCCATCTACTTTCTTAAGCTGAATCCTCCCAATGGAGTGCAAGTATTGGTATGGCCTCGGATTCTTCTTTTCTCTTGGTTTAGGCAGCACGTTTCTTACTACATCACCATAACCCCGCTCAAGAAAGCTCGTGGGAGGTATGATTTTGGGGAGATTGAATGGTCAGATGGGTTCCATAGGGTCACAAGTCCTCTGGTTGTTCGTGTTAGCAGCGCTAGCTAG

Coding sequence (CDS)

ATGTTGTATAGCTACAAACGTAGCTTTTCTGGGTTCTCCGCGAAGCTTAATGCAAGCCAAGCAATGACGTTATCGAAGATGGAAGGAGTGATATCCATATTCAAGAGTAAAACTATGGAGCTCCACACAACAAGAAGTTGGGACTTCTTGGGGCTTCCCATTCCTTCGTATAGCGGTAGTAGGACGTTTGATACACCACGTTCGAGTAAGCTGGCCGATGGCGACGACGTTGTTGTTGGCATCTTCGATTCAGGCATATGGCCTGAATCGGAAAGTTTCGAGGATGATCGGTGGATGTCCCCGGTTCCATGCAGCTGGAAGGGCAAATGCGTGAAAGCGTATAGATTCAACCCTTCATTAGCTTGCAATCGCAAGCTAATTGGCGCTCGTTACTATCTCAAAGGTTTCGAGGCCGAATACGGAGGACTCAACACAAGCGGGAACCCTGAGTTTGAATCGCCTCGAGACTTTTTGGGGCACGGAACTCATACAGCTTCAACGGCCGTGGGTGGAATTGTAGAGGACGTGAGCTTCACGGGGTCGTCACTCGGCAAAGGCATTGCTCGAGGAGGAGCGCCAAGAGCTAGGCTGGCTGTGTACAAGGTGTGCTGGGGAAAGGACTACGAAGGGAAGTGCACGGACGCAGACGTGATGGCTGCGTTTGACGACGCTTTATGCGACGGAGTCGACGTTATCTCGGCGTCGTTCGGAGGGTCGCCGCCGTTGGCTCCATTTTTTGAGTCTAGTAGTGCGATTGGGTCGTTCCATGGCATGCAGAAGGGAGTGAGTGTTGTGTTCTCAGCTGGTAACGATGGACCTTATCGTTCACTCGTTCAAAATGTGTCTCCGTGGAGCATTTGTGTTGCTGCTTCAACTATGGATCGAACCTTTCCCACTCAAATACTCATCCAAAACAGGATCTCTATTATGGGTGAGAGCTTGATCACTACAAACATAATTAACGCAAAACTAGCGAATGCGATCAACTATTTTATAGACGGAGTTTGCGAGCGGCGAAGTATAAGGAAAGGTAGGAAATCGGGGGCAGGGAAAGTGGTGGTTTGTTTCTCCACGGTAGGCCCAGTTTCGATGGGGGATGCACAAGAAGCTCTGGACGCCATCAATGCCTCGGCTTTGATATTTGGTGCCCCGCCCACGCTGCAGCTCCCTGACCTTGATCTCCTCCCCACCGTTCGAATTGACATTACTCACGCAACTCAAATTAGAAACTTTCTTGCTGAACTGCCCAGGCTGCCAGTAGTAGAGATCAGGGCTGCAAGAAGCGTGATTGGGAAGTCGGTGGCTCCGAGTGTGGCATACTTCTCGTCTAGAGGACCGAGCTCACTTTCGCCTGACATTCTTAAGCCAGACATAAGTGCCCCAGGAGTGAACATATTGGCGGCATGGCCTCCAGAAACTGCTCCAACAGTGCGGCCAACATCGACAGAAGGAGAAAGCCAAAGGCAAGTCAAATGGAATTTCCAATCAGGGACTTCAATGTCATGCCCTCACATCTCTGGAGTCGTTGCCCTCATCAAATCTCTGCATCCAAGCTGGTCTCCTGCAGCCATTAGATCTGCCCTCATCACCACAGCCACCAAGAGAGACTCCACTCGCAACACCATCCTAGCAGGTGGATCCACGAAACCATCCGATCCATTCGACATTGGAGGTGGCCAAGTGAACCCATTGAAGGCAGTAAATCCAGGACTAGTGTACGACATGACAGCCAATGATTACATTATTTTTCTCTGCAATATTGGGTACACAGAGCAGCAAATCAGGATGATTGTGAACCCTTCTCCACAAACACTTGTTTGTTGTCCACCATTTCTATCCACCTCCATTGCCAATCTCAATTACCCTTCCATTACACTTGCAAACCTCAAATCCACAACCACAATCAAAAGGACTGTTCGTAATGTAGCAGAAAACAAAAATGCCATCTACTTTCTTAAGCTGAATCCTCCCAATGGAGTGCAAGTATTGGTATGGCCTCGGATTCTTCTTTTCTCTTGGTTTAGGCAGCACGTTTCTTACTACATCACCATAACCCCGCTCAAGAAAGCTCGTGGGAGGTATGATTTTGGGGAGATTGAATGGTCAGATGGGTTCCATAGGGTCACAAGTCCTCTGGTTGTTCGTGTTAGCAGCGCTAGCTAG

Protein sequence

MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS
BLAST of Cp4.1LG05g08730 vs. Swiss-Prot
Match: SBT3I_ARATH (Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana GN=SBT3.18 PE=3 SV=2)

HSP 1 Score: 825.1 bits (2130), Expect = 6.0e-238
Identity = 419/723 (57.95%), Postives = 547/723 (75.66%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSY   F GFSAKLN++QA +L+K+  VI++FKSK+++LHTTRSWDFLGL + +   +
Sbjct: 68  MLYSYNNGFLGFSAKLNSTQAASLAKLNQVITVFKSKSLKLHTTRSWDFLGLAVDN---A 127

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
           R    P+   LA G D+VVGIFD+GIWPESESF +     P+P SW GKCV    F+PS+
Sbjct: 128 RRTPPPQ---LAYGSDIVVGIFDTGIWPESESFRETPEAKPIPSSWNGKCVGGEDFDPSV 187

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVS-FT 180
            CNRKLIGAR+YL+GFE  YG ++ + +PE+ SPRD+LGHGTHTASTAVG +V +VS F 
Sbjct: 188 HCNRKLIGARFYLRGFEETYGTIDFTRDPEYRSPRDYLGHGTHTASTAVGSVVRNVSGFF 247

Query: 181 GSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGS 240
           G  LG+G ARGGAP ARLAV+K CWGKD EG CT+AD++AAFDDA+ DGV VISASFG S
Sbjct: 248 G--LGRGTARGGAPLARLAVFKTCWGKDLEGVCTEADILAAFDDAIHDGVHVISASFGYS 307

Query: 241 PPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPT 300
           PPL+PFFESS+ IG+FH  ++G+SVVFS GNDGP   +VQNV+PW++ VAAST+DR+FPT
Sbjct: 308 PPLSPFFESSADIGAFHAAERGISVVFSTGNDGPDPGVVQNVAPWAVSVAASTVDRSFPT 367

Query: 301 QILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTV 360
           +I+I    ++ G+SLI+   I   LA A  YF  GVC+  +  K  K     +++CFST+
Sbjct: 368 RIVIDGSFTLTGQSLISQE-ITGTLALATTYFNGGVCKWENWMK--KLANETIILCFSTL 427

Query: 361 GPVS-MGDAQEALDAINASALIFGAPPTLQL-PDLDLLPTVRIDITHATQIRNFLAELPR 420
           GPV  + +AQ A    NA ALIF A PT QL  ++D++PTVR+DI H T+IRN+LA  P 
Sbjct: 428 GPVQFIEEAQAAAIRANALALIFAASPTRQLAEEVDMIPTVRVDILHGTRIRNYLARSPT 487

Query: 421 LPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTV 480
           +P+V+I  +++VIG++ APSVAYFSSRGPSSLSPDILKPDI+APG+ ILAAWPP T PT+
Sbjct: 488 VPMVKIGPSKTVIGETTAPSVAYFSSRGPSSLSPDILKPDITAPGIGILAAWPPRTPPTL 547

Query: 481 RPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDS 540
            P        R ++WNFQSGTSMSCPH++GV+AL++S HP WSP+AIRSA++TTA  RD+
Sbjct: 548 LP-----GDHRSIEWNFQSGTSMSCPHVAGVMALLQSAHPDWSPSAIRSAIMTTAYTRDT 607

Query: 541 TRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIR-M 600
           + + IL+GGS K +DPFDIG G +NPLKA++PGLVY+   +DY++F+CNIGYT+Q+I+ M
Sbjct: 608 SYDLILSGGSMKSTDPFDIGAGHINPLKAMDPGLVYNTRTDDYVLFMCNIGYTDQEIKSM 667

Query: 601 IVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNP 660
           +++P P T  C P     + A+ NYPSIT+ +L+ T TIKRTV NV  NKN +YF+ +  
Sbjct: 668 VLHPEPST-TCLPSHSYRTNADFNYPSITIPSLRLTRTIKRTVSNVGPNKNTVYFVDIIR 727

Query: 661 PNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRV 720
           P GV+VL+WPRIL+FS  +Q  SYY+T  P +   GRY FGEI W++G HRV SP+VV +
Sbjct: 728 PVGVEVLIWPRILVFSKCQQEHSYYVTFKPTEIFSGRYVFGEIMWTNGLHRVRSPVVVFL 773

BLAST of Cp4.1LG05g08730 vs. Swiss-Prot
Match: SBT39_ARATH (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 8.6e-152
Identity = 309/718 (43.04%), Postives = 432/718 (60.17%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           ++YSY+  FSGF+AKL  SQA  +S++  V+ +  +   E+ TTR+WD+LG+  P  S S
Sbjct: 67  IVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDS 126

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
                    K   G +V+VG+ DSG+WPESE F +D+   P+P  WKG C     FN S+
Sbjct: 127 LL------QKANMGYNVIVGVIDSGVWPESEMF-NDKGFGPIPSRWKGGCESGELFNASI 186

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CNRKLIGA+Y++ G  AE+G +N + NPE+ SPRDF GHGTH AST  G  + +VS+ G
Sbjct: 187 HCNRKLIGAKYFVDGLVAEFGVVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVG 246

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSP 240
             LG+G ARGGAP   +AVYK CW     G C+ ADV+ A D+A+ DGVD++S S G S 
Sbjct: 247 --LGRGTARGGAPGVHIAVYKACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSV 306

Query: 241 PLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQ 300
           PL P  E +S +G+FH + KG+ VV +AGN GP    + NV+PW + VAA+T DR+FPT 
Sbjct: 307 PLFPETEHTS-VGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTA 366

Query: 301 ILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVG 360
           I + N I+I+G+++     +           + G CE+ S      +  GKVV+CF+   
Sbjct: 367 ITLGNNITILGQAIYGGPELGFVGLTYPESPLSGDCEKLSANP-NSTMEGKVVLCFAAST 426

Query: 361 PVSMGDAQEALDAINAS--ALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
           P +   A      INA    LI    PT  L      P V ID    T I  F     R 
Sbjct: 427 PSNAAIAA----VINAGGLGLIMAKNPTHSLTPTRKFPWVSIDFELGTDIL-FYIRSTRS 486

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+V+I+A++++ G+SV+  VA FSSRGP+S+SP ILKPDI+APGVNILAA  P +     
Sbjct: 487 PIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNS----- 546

Query: 481 PTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDST 540
                  S     +   SGTSM+ P +SGVV L+KSLHP WSP+AI+SA++TTA + D +
Sbjct: 547 -------SINDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPS 606

Query: 541 RNTILAGGST-KPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMI 600
              I A GS+ K +DPFD GGG +NP KAV PGL+YDMT +DY++++C++ Y++  I  +
Sbjct: 607 GEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRV 666

Query: 601 VNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPP 660
           +    +  VC  P    S+ +LN PSIT+ NL+   T+ RTV NV    N++Y + ++PP
Sbjct: 667 LG---KITVCPNP--KPSVLDLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPP 726

Query: 661 NGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 716
            G+ V V P  L+F +     S+ + ++   K    Y FG + W+D  H V  P+ VR
Sbjct: 727 TGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVR 745

BLAST of Cp4.1LG05g08730 vs. Swiss-Prot
Match: SBT35_ARATH (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 5.2e-149
Identity = 311/728 (42.72%), Postives = 437/728 (60.03%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           M+YSY+  FSGF+AKL  SQA  L+    V+ +      EL TTR+WD+LGL + + +  
Sbjct: 67  MVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPNNL 126

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
              DT        GD V++G  D+G+WPESESF D+  + P+P  WKG C    +F  S 
Sbjct: 127 LN-DTNM------GDQVIIGFIDTGVWPESESFNDNG-VGPIPSHWKGGCESGEKFI-ST 186

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CNRKLIGA+Y++ GF AE  G NT+ + ++ S RDF+GHGTHTAS A G  V ++S+ G
Sbjct: 187 NCNRKLIGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKG 246

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYEG--KCTDADVMAAFDDALCDGVDVISASFGG 240
             L  G  RGGAPRAR+A+YK CW  D  G   C+ +D++ A D+++ DGVDV+S S G 
Sbjct: 247 --LAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGA 306

Query: 241 SPPLAP--FFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRT 300
             PL P        A G+FH + KG+ VV + GN GP    V N +PW I VAA+T+DR+
Sbjct: 307 QIPLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRS 366

Query: 301 FPTQILIQNRISIMGESLIT------TNIINAKLANAINYFIDGVCERRSIRKGRKSGAG 360
           FPT I + NR  I+G++L T      T+++  + A   N    GVCER ++   R + AG
Sbjct: 367 FPTPITLGNRKVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNLNPNR-TMAG 426

Query: 361 KVVVCFST-VGPVSMGDAQEALDAINASALIFGAPPTLQL-PDLDLLPTVRIDITHATQI 420
           KVV+CF+T     ++  A   + A     +I    P   L P  D  P V ID    T +
Sbjct: 427 KVVLCFTTNTLFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDV 486

Query: 421 RNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAA 480
             ++    R PVV+I+ +R+++G+ V   VA FSSRGP+S+SP ILKPDI APGV+ILAA
Sbjct: 487 LLYIRS-TRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAA 546

Query: 481 WPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSAL 540
             P++  +V              ++  +GTSM+ P ++GVVAL+K+LHP+WSPAA RSA+
Sbjct: 547 TSPDSNSSVG------------GFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAI 606

Query: 541 ITTATKRDSTRNTILA-GGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNI 600
           +TTA + D     I A G S K +DPFD GGG VNP KA +PGL+YDM   DYI++LC+ 
Sbjct: 607 VTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSA 666

Query: 601 GYTEQQIRMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKN 660
           GY +  I  +V       VC  P   TS+ ++N PSIT+ +LK   T+ RTV NV    +
Sbjct: 667 GYNDSSITQLVG---NVTVCSTP--KTSVLDVNLPSITIPDLKDEVTLTRTVTNVG-TVD 726

Query: 661 AIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHR 716
           ++Y + + PP G+QV+V P  L+F+   ++VS+ + ++   K    + FG + W+D  H 
Sbjct: 727 SVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHN 763

BLAST of Cp4.1LG05g08730 vs. Swiss-Prot
Match: SBT3A_ARATH (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 520.4 bits (1339), Expect = 3.2e-146
Identity = 301/718 (41.92%), Postives = 429/718 (59.75%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           ++YSY+  FSGF+AKL  SQA  +S++  V+ +  +   E+ TTR+WD+LG+  P  S S
Sbjct: 67  IVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDS 126

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
                    K   G +V+VG+ D+G+WPESE F +D+   P+P  WKG C     FN S+
Sbjct: 127 LL------QKANMGYNVIVGVIDTGVWPESEMF-NDKGYGPIPSRWKGGCESGELFNGSI 186

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CNRKLIGA+Y++    A++G LN + NP++ SPRDF GHGTH AST  G  + +VS+ G
Sbjct: 187 HCNRKLIGAKYFIDANNAQFGVLNKTENPDYLSPRDFNGHGTHVASTIGGSFLPNVSYLG 246

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSP 240
             LG+G ARGGAP   +AVYK CW    +  C+ ADV+ A D+A+ DGVD++S S   S 
Sbjct: 247 --LGRGTARGGAPGVHIAVYKACW---VQRGCSGADVLKAMDEAIHDGVDILSLSLQTSV 306

Query: 241 PLAPFFESS--SAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFP 300
           PL P  ++   +++G+FH + KG+ VV +A N GP    + NV+PW + VAA+T DR+FP
Sbjct: 307 PLFPETDARELTSVGAFHAVAKGIPVVAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFP 366

Query: 301 TQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFST 360
           T I + N I+I+G+++   + +           + G CE+ S    + +  GKVV+CF+ 
Sbjct: 367 TAITLGNNITILGQAIFGGSELGFVGLTYPESPLSGDCEKLSANP-KSAMEGKVVLCFAA 426

Query: 361 VGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
             P +   A  A+       LI    PT  L  L   P V +D    T I  F     R 
Sbjct: 427 STPSNA--AITAVINAGGLGLIMARNPTHLLRPLRNFPYVSVDFELGTDIL-FYIRSTRS 486

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+V I+A+R++ G+SV+  VA FSSRGP+S+SP ILKPDI+APGVNILAA  P       
Sbjct: 487 PIVNIQASRTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISP------- 546

Query: 481 PTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDST 540
                  S     +   SGTSM+ P +SGVV L+KSLHP WSP+AI+SA++TTA + D +
Sbjct: 547 ------NSINDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPS 606

Query: 541 RNTILAGGST-KPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMI 600
              I A GS+ K +DPFD GGG +NP KAV PGL+YDMT +DY++++C++ Y++  I  +
Sbjct: 607 GEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRV 666

Query: 601 VNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPP 660
           +    +  VC  P    S+ +LN PSIT+ NL+   T+ RTV NV    N++Y + ++PP
Sbjct: 667 LG---KITVCPNP--KPSVLDLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPP 726

Query: 661 NGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 716
            GV V V P  L+F       S+ + ++   K    Y FG + W+D  H V  P+ VR
Sbjct: 727 TGVNVAVTPTELVFDSTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDTLHNVAIPVSVR 748

BLAST of Cp4.1LG05g08730 vs. Swiss-Prot
Match: SBT33_ARATH (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.8e-142
Identity = 308/728 (42.31%), Postives = 423/728 (58.10%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           M+YSY+  FSGF+AKL  SQA  ++ +  V+ +      EL TTR+W++LGL     S +
Sbjct: 70  MVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGL-----SSA 129

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
              +    + +  GD V++G+ D+G+WPESESF D+  + P+P  WKG C     F  S 
Sbjct: 130 NPKNLLNDTNM--GDQVIIGVIDTGVWPESESFNDNG-VGPIPRKWKGGCESGENFR-ST 189

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CNRKLIGA+Y++ GF AE  G NT+ + ++ S RDF GHGTH AS A G  V +VS+ G
Sbjct: 190 DCNRKLIGAKYFINGFLAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKG 249

Query: 181 SSLGKGIARGGAPRARLAVYKVCW-GKDYEG-KCTDADVMAAFDDALCDGVDVISASFGG 240
             L  G  RGGAPRAR+A+YK CW  ++ +G  C+D+D+M A D+A+ DGVDV+S S  G
Sbjct: 250 --LAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVG 309

Query: 241 SPPL--APFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRT 300
             PL          A G FH + KG+ VV + GNDGP    V N++PW + VAA+T+DR+
Sbjct: 310 QIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRS 369

Query: 301 FPTQILIQNRISIMGESLIT------TNIINAKLANAINYFIDGVCERRSIRKGRKSGAG 360
           FPT I + N   I+G++  T      T+++  + A   N    GVCE  ++     + A 
Sbjct: 370 FPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNP-NYTMAM 429

Query: 361 KVVVCFSTVGP-VSMGDAQEALDAINASALIFGAPPTLQL-PDLDLLPTVRIDITHATQI 420
           KVV+CF+      ++  A   + A     LI    P   L P  D  P V +D    T I
Sbjct: 430 KVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDI 489

Query: 421 RNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAA 480
            +++    R PVV+I+ +R++ G+ V   V  FSSRGP+S+SP ILKPDI+APGV ILAA
Sbjct: 490 LSYIRS-TRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 549

Query: 481 WPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSAL 540
             P     V              +   SGTSM+ P ISGV+AL+K+LHP WSPAA RSA+
Sbjct: 550 TSPNDTLNVG------------GFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAI 609

Query: 541 ITTATKRDSTRNTILA-GGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNI 600
           +TTA + D     I A G S K SDPFD GGG VNP KA  PGL+YDM   DYI++LC+ 
Sbjct: 610 VTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSA 669

Query: 601 GYTEQQIRMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKN 660
           GY +  I  +V    Q  VC  P    S+ ++N PSIT+ NLK   T+ RTV NV    +
Sbjct: 670 GYNDSSISQLVG---QITVCSNP--KPSVLDVNLPSITIPNLKDEVTLTRTVTNVG-LVD 729

Query: 661 AIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHR 716
           ++Y + + PP GV+V+V P  L+F+     VS+ + ++   K    Y FG + W+D  H 
Sbjct: 730 SVYKVSVEPPLGVRVVVTPETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHN 766

BLAST of Cp4.1LG05g08730 vs. TrEMBL
Match: A0A0A0LVP7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G153520 PE=4 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 553/725 (76.28%), Postives = 618/725 (85.24%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSYK+SFSGFSAKLNASQA+ LSKME VIS+F+S+TM+LHTTRSWDFLGLPIPSY+ +
Sbjct: 21  MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMKLHTTRSWDFLGLPIPSYTNN 80

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
           R+             DVVV IFDSG+WPES+SFE+   +  +PC+WKGKCVK YRFNP+ 
Sbjct: 81  RSARFSLHLPSYGDHDVVVAIFDSGVWPESKSFEESEGIGRIPCNWKGKCVKGYRFNPAS 140

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSG-NPEFESPRDFLGHGTHTASTAVGGIVEDVSF- 180
           ACNRKLIGARYYLKGFEA+YG LNT+  NPEF SPRDFLGHGTHTASTAVG +V +V F 
Sbjct: 141 ACNRKLIGARYYLKGFEAQYGALNTTADNPEFRSPRDFLGHGTHTASTAVGAVVHNVGFP 200

Query: 181 TGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGG 240
           T SSL KG ARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG 
Sbjct: 201 TSSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGE 260

Query: 241 SPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFP 300
            PPL P FES+SAIGSFH MQ+GVSVVFSAGNDG + SLVQNVSPWSICVAASTMDRTFP
Sbjct: 261 RPPLIPLFESASAIGSFHAMQRGVSVVFSAGNDGSHPSLVQNVSPWSICVAASTMDRTFP 320

Query: 301 TQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFST 360
           T I I N  SIMGESLIT NIIN KLA+AINYF DG+CER +IRKG KSG GKVVVCFST
Sbjct: 321 TTIFILNHFSIMGESLITRNIINVKLADAINYFNDGICERENIRKGGKSGKGKVVVCFST 380

Query: 361 VGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
           +G VS+  AQEA+ AINASALIFGAPPT +LPDLDL+PTVRIDI  ATQIRNFLAELPRL
Sbjct: 381 IGQVSIATAQEAVKAINASALIFGAPPTTELPDLDLIPTVRIDIHQATQIRNFLAELPRL 440

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+VEI  ARSVIGKSVAP+VAYFSSRGPSS+ PDILKPDISAPGVNILAAWPPETAPTVR
Sbjct: 441 PMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVR 500

Query: 481 PT----STEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATK 540
           P+      E E +  VKWNFQSGTSMSCPH+SGVVALIKS+HP+WSPAAIRSA+ITTATK
Sbjct: 501 PSGKINEEEEEEEEGVKWNFQSGTSMSCPHVSGVVALIKSVHPNWSPAAIRSAIITTATK 560

Query: 541 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 600
            DS+ NTILAGGS K SDPFDIG GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI
Sbjct: 561 IDSSGNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQI 620

Query: 601 -RMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLK 660
             +I+NPSP    CC    +T IAN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL+
Sbjct: 621 SNLILNPSPH--FCCRQSTAT-IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLR 680

Query: 661 LNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLV 719
           + PP GV+V VWPR+L FS +RQ +SYYITITPL+K+RGRY FGEI+W + FH VTSPLV
Sbjct: 681 VLPPYGVRVQVWPRVLFFSCYRQQISYYITITPLRKSRGRYGFGEIQWFNRFHTVTSPLV 740

BLAST of Cp4.1LG05g08730 vs. TrEMBL
Match: A0A067EU78_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g003970mg PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 1.6e-258
Identity = 448/720 (62.22%), Postives = 566/720 (78.61%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           +LY YK SFSGFSAKLN+SQA +L++ME VISIF+S+ ++LHTTRSWDF+GL + + +G 
Sbjct: 67  LLYGYKYSFSGFSAKLNSSQAASLAEMEEVISIFESQVLKLHTTRSWDFMGLILDN-TGE 126

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
            T       +LA GDD+VVGIFD+G+WPESESF+++  MSP+P SWKG CV+  +F+P  
Sbjct: 127 VT-----PVQLAYGDDIVVGIFDTGVWPESESFQEEPSMSPIPSSWKGTCVRGEKFDPQK 186

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
           ACNRKLIGARYY+KGFE EYG LN S N E+ S RDFLGHGTHTASTA G I ++  F G
Sbjct: 187 ACNRKLIGARYYVKGFEEEYGPLNASTNREYRSARDFLGHGTHTASTAAGSIAKNAGFFG 246

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSP 240
             LG+GIARGGAPRARLAVYK+CWGKD +GKCT+AD++AAFDDAL DGVDVISASFG SP
Sbjct: 247 --LGRGIARGGAPRARLAVYKICWGKDSDGKCTEADILAAFDDALHDGVDVISASFGESP 306

Query: 241 PLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQ 300
           PL PFF S++ IGSF+ MQ GV+VVFS GNDGP  SLVQNV+PWSICVAAS++DRTFPT+
Sbjct: 307 PLRPFFASNADIGSFNAMQHGVTVVFSGGNDGPEPSLVQNVAPWSICVAASSIDRTFPTE 366

Query: 301 ILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVG 360
           I++ +  SI+GES I+T  + AKL  A  YF DG+C+  +   GRK+  G+VV+CFST+G
Sbjct: 367 IVVNSDFSIVGESFISTE-VKAKLVEAFTYFADGICKCEN-WMGRKA-TGRVVLCFSTMG 426

Query: 361 PVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPV 420
            V   +A+ A    NAS LIF  P T  + ++D++PTVRIDI   TQ+R++LA+ PRLP+
Sbjct: 427 SVKTEEAEAAAKKANASGLIFAEPMTELIAEVDIIPTVRIDIAQGTQLRDYLAQFPRLPI 486

Query: 421 VEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVRPT 480
           V+++ +++ IGK  AP+VAYFSSRGPSS+SPDILKPDI+APG+ +LAAWPP T PT+ P+
Sbjct: 487 VQLKPSKTSIGKVPAPTVAYFSSRGPSSISPDILKPDITAPGIGVLAAWPPNTPPTLLPS 546

Query: 481 STEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDSTRN 540
                  R VKWNFQSGTSMSCPH+SGVVALIKS HP+WSPAAIRSAL+TTA  RD++ +
Sbjct: 547 -----DGRSVKWNFQSGTSMSCPHVSGVVALIKSAHPNWSPAAIRSALMTTAYTRDTSHD 606

Query: 541 TILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNP 600
           +ILAGGS K SDPFDIG G +NP+KA++PGL+YD+   DYI+FL NIGYT+ QI  I  P
Sbjct: 607 SILAGGSMKVSDPFDIGAGHINPMKAMDPGLIYDLKPTDYIVFLRNIGYTQDQINKIFLP 666

Query: 601 SPQTL--VCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPPN 660
           SP       CP       + +NYPSIT++NL+ST TIKRTV+NV + KNAIYF  +  P 
Sbjct: 667 SPDETERTSCPQAHKIPNSFINYPSITVSNLQSTMTIKRTVKNVGQKKNAIYFASVVKPG 726

Query: 661 GVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 719
           GV+V+VWPR+L+FSWF++ VSYY+++ PLK ++GR+DFG+I WSDGFH V SPLVV V++
Sbjct: 727 GVEVVVWPRVLVFSWFKEEVSYYVSLKPLKMSQGRFDFGQIVWSDGFHYVRSPLVVFVNN 770

BLAST of Cp4.1LG05g08730 vs. TrEMBL
Match: M5WNJ5_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa017229mg PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 3.4e-256
Identity = 444/719 (61.75%), Postives = 563/719 (78.30%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSYK SFSGFSAKLN+SQA TL+ M+ VIS+F+SKT+ LHTTRSWDFLGL + S S +
Sbjct: 42  MLYSYKHSFSGFSAKLNSSQATTLANMDEVISVFRSKTLRLHTTRSWDFLGLTLSSSSST 101

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
            T  TP   +LA G+D++VGIFD+GIWPES+SF ++  M P+P +WKG+CVK   F P+ 
Sbjct: 102 STAGTPL--QLAYGEDIIVGIFDTGIWPESDSFREEPRMRPIPSTWKGRCVKGEMFEPAK 161

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVED-VSFT 180
           ACNRKLIGARYYL+GFE EYG LNTSGNPE+ S RDFLGHGTHTASTAVG IV++  SF 
Sbjct: 162 ACNRKLIGARYYLRGFEEEYGPLNTSGNPEYRSARDFLGHGTHTASTAVGSIVKNNASFF 221

Query: 181 GSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGS 240
           G  L +G ARGGAPRARLAVYKVCWGKDY+GKC++AD++A FDDAL D V VISASFG  
Sbjct: 222 G--LAQGTARGGAPRARLAVYKVCWGKDYDGKCSEADILAGFDDALHDRVHVISASFGSK 281

Query: 241 PPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPT 300
           PPL P F S +AIGSFH MQ GV+VVFSAGN+GP  S V NV+PW ICVAAS++DR FPT
Sbjct: 282 PPLLPLFVSEAAIGSFHAMQLGVTVVFSAGNEGPDPSQVTNVAPWGICVAASSVDRMFPT 341

Query: 301 QILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTV 360
           +I + N++S MGES I T  I  KLA+A  YF++GVC   +  +  K   G+V++CFS++
Sbjct: 342 RITLDNKLSFMGESFIITP-IKGKLADATTYFVNGVCRPENWIQS-KFAVGRVILCFSSI 401

Query: 361 GPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLP 420
           GP  + +A+ A    NASA+IF    T Q+  +D++P+V +++   T+I+++LA+ P  P
Sbjct: 402 GPYEIDEAEAAAKKANASAVIFVEAMTRQVA-VDIIPSVHVNLEQGTKIKHYLAQSPTKP 461

Query: 421 VVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVRP 480
           VV+I ++++VIGKS+AP VAYFSSRGPSS++PDILKPDISAPGVNILAAWPP+T+PT+  
Sbjct: 462 VVQIESSKTVIGKSLAPRVAYFSSRGPSSITPDILKPDISAPGVNILAAWPPQTSPTLTL 521

Query: 481 TSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDSTR 540
                + +R V WNFQSGTSMSCPH+SGVVALIKS HP+WSPAAIRSA+ITTA  RD++ 
Sbjct: 522 -----DDKRSVSWNFQSGTSMSCPHVSGVVALIKSAHPTWSPAAIRSAIITTAYTRDTSF 581

Query: 541 NTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVN 600
           + I+A GS K SDP D G G ++P+KA++PGLVYDM  +DYI+FLCNIGYTE QI  IV 
Sbjct: 582 DRIVADGSMKASDPLDFGAGHIDPIKAMDPGLVYDMKTSDYILFLCNIGYTEDQISQIVL 641

Query: 601 PSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPPNG 660
               T   CP  L +++ NLNYPSIT ANL+ST TIKR+VRNV +NKN IYF  ++ P+G
Sbjct: 642 CPSGTDTSCPQVLQSNV-NLNYPSITFANLQSTVTIKRSVRNVGKNKNVIYFCTISEPDG 701

Query: 661 VQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 719
           V+V++WPR+L+FSWF+   +Y++T+ P K+++GRYDFGEI WSDG H+V SPLVV V++
Sbjct: 702 VEVVIWPRVLIFSWFKVESTYFVTLKPKKESQGRYDFGEIVWSDGLHKVRSPLVVSVNT 747

BLAST of Cp4.1LG05g08730 vs. TrEMBL
Match: A0A061GYM5_THECC (Subtilisin-like serine endopeptidase family protein OS=Theobroma cacao GN=TCM_039861 PE=4 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 7.1e-254
Identity = 442/721 (61.30%), Postives = 560/721 (77.67%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           +LYSY+ SFSGFSAKLN+SQA TL+KME V+S+F+SK ++LHTTRSWDFLGLP+  Y   
Sbjct: 67  ILYSYRNSFSGFSAKLNSSQATTLAKMENVVSVFRSKILKLHTTRSWDFLGLPL--YDSE 126

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
            T       +L  G DVVVGIFD+GIWPESESF++   M P+P SWKG CVK  +F P+ 
Sbjct: 127 VT-----PLQLTYGHDVVVGIFDTGIWPESESFQEKPGMGPIPSSWKGVCVKGEKFEPAR 186

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
           ACNRKLIGARYYLKGFE  +G LNTSGNPE+ S RDFLGHGTHTASTA G IV+   F G
Sbjct: 187 ACNRKLIGARYYLKGFEQAFGPLNTSGNPEYRSARDFLGHGTHTASTAAGSIVKSAGFFG 246

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSP 240
             +G+GIARGGAP AR+AVYK+CWGK+  G+CT+AD++AAFDDAL DGV VISASFG +P
Sbjct: 247 --IGEGIARGGAPWARVAVYKICWGKNRVGRCTEADILAAFDDALHDGVHVISASFGATP 306

Query: 241 PLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQ 300
           PL+PFF SS+ IGSFH MQ G++ VFSAGNDGP  SLVQNV+PWS+CVAAST+DR+FPT+
Sbjct: 307 PLSPFFASSADIGSFHAMQLGITAVFSAGNDGPDPSLVQNVAPWSVCVAASTIDRSFPTE 366

Query: 301 ILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVG 360
           I++ +  SIMGES+I+   I  KLANA+ YF++GVC   + +  +K   GK+V+CF   G
Sbjct: 367 IVLDSNFSIMGESIISKE-IKGKLANAVTYFVNGVCNLGNWK--QKLATGKIVLCFYIPG 426

Query: 361 PVSMGDAQEALDAINASALIFGAPPTLQLPDL-DLLPTVRIDITHATQIRNFLAELPRLP 420
            V    AQEA+   N   LIF  P T  + ++ D +PTV +DI+  T I N+LAE P+LP
Sbjct: 427 LV-FEMAQEAVKTANGLGLIFAEPLTKPIAEVEDTIPTVHVDISQGTIIGNYLAESPKLP 486

Query: 421 VVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVRP 480
           +V I  ++++IGKS AP+V YFSSRGPSS+SPDILKPDI+APG+NILAAWP +T PT+ P
Sbjct: 487 LVRIAPSKTIIGKSPAPTVVYFSSRGPSSISPDILKPDITAPGINILAAWPCQTPPTLLP 546

Query: 481 TSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDSTR 540
                   R VKWNFQSGTSMSCPH+SGVVAL+KS+HP WSPAAIRSA++T+A  RD++ 
Sbjct: 547 -----GDNRSVKWNFQSGTSMSCPHVSGVVALVKSVHPDWSPAAIRSAIMTSAYTRDTSH 606

Query: 541 NTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVN 600
           ++ILAGGS   S PFD+G G +NPL+A++PGL+YDM  +DYI+FLCN+GYT+++I+ +V 
Sbjct: 607 DSILAGGSVAGSSPFDLGAGHINPLRAIDPGLIYDMKTSDYILFLCNMGYTQERIKRMVL 666

Query: 601 PSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPPNG 660
           PSP     C   + T+ AN+NYPSI+++NL+ST TIKRT+RNV   KNAIYF     P+G
Sbjct: 667 PSPGVDTSCEHVVKTN-ANVNYPSISISNLQSTMTIKRTLRNVGWKKNAIYFGTTKEPDG 726

Query: 661 VQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSA 720
           V V++WPR+L+FS  +Q +SYY+T+ PLKK++GRYDFGEI WSDGFH V SPLVV V++A
Sbjct: 727 VAVVIWPRVLIFSPLKQDISYYVTLKPLKKSQGRYDFGEIVWSDGFHYVRSPLVVLVNTA 768

BLAST of Cp4.1LG05g08730 vs. TrEMBL
Match: V4TYX3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014478mg PE=4 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.5e-248
Identity = 430/694 (61.96%), Postives = 543/694 (78.24%), Query Frame = 1

Query: 27  MEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSSKLADGDDVVVGIFDSGI 86
           ME VISIF+S+ ++LHTTRSWDF+GL + + +G  T       +LA GDD+VVGIFD+G+
Sbjct: 1   MEEVISIFESQVLKLHTTRSWDFMGLILDN-TGEVT-----PVQLAYGDDIVVGIFDTGV 60

Query: 87  WPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEYGGLNTS 146
           WPESESF+++  MSP+P SWKG CV+  +F+P  ACNRKLIGARYY+KGFE EYG LN S
Sbjct: 61  WPESESFQEEPSMSPIPSSWKGTCVRGEKFDPQKACNRKLIGARYYVKGFEEEYGPLNAS 120

Query: 147 GNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTGSSLGKGIARGGAPRARLAVYKVCWGK 206
            N E+ S RDFLGHGTHTASTA G I ++  F G  LG+GIARGGAPRARLAVYK+CWGK
Sbjct: 121 TNREYRSARDFLGHGTHTASTAAGSIAKNAGFFG--LGRGIARGGAPRARLAVYKICWGK 180

Query: 207 DYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQKGVSVVF 266
           D +GKCT+AD++AAFDDAL DGVDVISASFG SPPL PFF S++ IGSF+ MQ GV+VVF
Sbjct: 181 DSDGKCTEADILAAFDDALHDGVDVISASFGESPPLRPFFASNADIGSFNAMQHGVTVVF 240

Query: 267 SAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTNIINAKLAN 326
           S GNDGP  SLVQNV+PWSICVAAS++DRTFPT+I++ +  SI+GES I+T  + AKL  
Sbjct: 241 SGGNDGPEPSLVQNVAPWSICVAASSIDRTFPTEIVVNSDFSIVGESFISTE-VKAKLVE 300

Query: 327 AINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVGPVSMGDAQEALDAINASALIFGAPPT 386
           A  YF DG+C+  +   GRK+  G+VV+CFST+G V   +A+ A    NAS LIF  P T
Sbjct: 301 AFTYFADGICKCEN-WMGRKA-TGRVVLCFSTMGSVKTEEAEAAAKKANASGLIFAEPMT 360

Query: 387 LQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGP 446
             + ++D++PTVRIDI   TQ+R++LA+ PRLP+V+++ +++ IGK  AP+VAYFSSRGP
Sbjct: 361 ELIAEVDIIPTVRIDIAQGTQLRDYLAQFPRLPIVQLKPSKTSIGKVPAPTVAYFSSRGP 420

Query: 447 SSLSPDILKPDISAPGVNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHIS 506
           SS+SPDILKPDI+APG+ +LAAWPP T PT+ P+       R VKWNFQSGTSMSCPH+S
Sbjct: 421 SSISPDILKPDITAPGIGVLAAWPPNTPPTLLPS-----DGRSVKWNFQSGTSMSCPHVS 480

Query: 507 GVVALIKSLHPSWSPAAIRSALITTATKRDSTRNTILAGGSTKPSDPFDIGGGQVNPLKA 566
           GVVALIKS HP+WSPAAIRSAL+TTA  RD++ ++ILAGGS K SDPFDIG G +NP+KA
Sbjct: 481 GVVALIKSAHPNWSPAAIRSALMTTAYTRDTSHDSILAGGSMKVSDPFDIGAGHINPMKA 540

Query: 567 VNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTL--VCCPPFLSTSIANLNYPSI 626
           ++PGL+YD+   DYI+FL NIGYT+ QI  I  PSP       CP       + +NYPSI
Sbjct: 541 MDPGLIYDLKPTDYIVFLRNIGYTQDQINKIFLPSPDETERTSCPQAHKIPNSFINYPSI 600

Query: 627 TLANLKSTTTIKRTVRNVAENKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITI 686
           T++NL+ST TIKRTV+NV + KNAIYF  +  P GV+V+VWPR+L+FSWF++ VSYY+++
Sbjct: 601 TVSNLQSTMTIKRTVKNVGQKKNAIYFASVVKPGGVEVVVWPRVLVFSWFKEEVSYYVSL 660

Query: 687 TPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 719
            PLK ++GR+DFG+I WSDGFH V SPLVV V++
Sbjct: 661 KPLKMSQGRFDFGQIVWSDGFHYVRSPLVVFVNN 678

BLAST of Cp4.1LG05g08730 vs. TAIR10
Match: AT4G26330.1 (AT4G26330.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 815.5 bits (2105), Expect = 2.7e-236
Identity = 419/737 (56.85%), Postives = 547/737 (74.22%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSY   F GFSAKLN++QA +L+K+  VI++FKSK+++LHTTRSWDFLGL + +   +
Sbjct: 21  MLYSYNNGFLGFSAKLNSTQAASLAKLNQVITVFKSKSLKLHTTRSWDFLGLAVDN---A 80

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSG--------------IWPESESFEDDRWMSPVPCSW 120
           R    P+   LA G D+VVGIFD+G              IWPESESF +     P+P SW
Sbjct: 81  RRTPPPQ---LAYGSDIVVGIFDTGLFISLKLLLLSILGIWPESESFRETPEAKPIPSSW 140

Query: 121 KGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTAS 180
            GKCV    F+PS+ CNRKLIGAR+YL+GFE  YG ++ + +PE+ SPRD+LGHGTHTAS
Sbjct: 141 NGKCVGGEDFDPSVHCNRKLIGARFYLRGFEETYGTIDFTRDPEYRSPRDYLGHGTHTAS 200

Query: 181 TAVGGIVEDVS-FTGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDAL 240
           TAVG +V +VS F G  LG+G ARGGAP ARLAV+K CWGKD EG CT+AD++AAFDDA+
Sbjct: 201 TAVGSVVRNVSGFFG--LGRGTARGGAPLARLAVFKTCWGKDLEGVCTEADILAAFDDAI 260

Query: 241 CDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWS 300
            DGV VISASFG SPPL+PFFESS+ IG+FH  ++G+SVVFS GNDGP   +VQNV+PW+
Sbjct: 261 HDGVHVISASFGYSPPLSPFFESSADIGAFHAAERGISVVFSTGNDGPDPGVVQNVAPWA 320

Query: 301 ICVAASTMDRTFPTQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGR 360
           + VAAST+DR+FPT+I+I    ++ G+SLI+   I   LA A  YF  GVC+  +  K  
Sbjct: 321 VSVAASTVDRSFPTRIVIDGSFTLTGQSLISQE-ITGTLALATTYFNGGVCKWENWMK-- 380

Query: 361 KSGAGKVVVCFSTVGPVS-MGDAQEALDAINASALIFGAPPTLQL-PDLDLLPTVRIDIT 420
           K     +++CFST+GPV  + +AQ A    NA ALIF A PT QL  ++D++PTVR+DI 
Sbjct: 381 KLANETIILCFSTLGPVQFIEEAQAAAIRANALALIFAASPTRQLAEEVDMIPTVRVDIL 440

Query: 421 HATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGV 480
           H T+IRN+LA  P +P+V+I  +++VIG++ APSVAYFSSRGPSSLSPDILKPDI+APG+
Sbjct: 441 HGTRIRNYLARSPTVPMVKIGPSKTVIGETTAPSVAYFSSRGPSSLSPDILKPDITAPGI 500

Query: 481 NILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAA 540
            ILAAWPP T PT+ P        R ++WNFQSGTSMSCPH++GV+AL++S HP WSP+A
Sbjct: 501 GILAAWPPRTPPTLLP-----GDHRSIEWNFQSGTSMSCPHVAGVMALLQSAHPDWSPSA 560

Query: 541 IRSALITTATKRDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIF 600
           IRSA++TTA  RD++ + IL+GGS K +DPFDIG G +NPLKA++PGLVY+   +DY++F
Sbjct: 561 IRSAIMTTAYTRDTSYDLILSGGSMKSTDPFDIGAGHINPLKAMDPGLVYNTRTDDYVLF 620

Query: 601 LCNIGYTEQQIR-MIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNV 660
           +CNIGYT+Q+I+ M+++P P T  C P     + A+ NYPSIT+ +L+ T TIKRTV NV
Sbjct: 621 MCNIGYTDQEIKSMVLHPEPST-TCLPSHSYRTNADFNYPSITIPSLRLTRTIKRTVSNV 680

Query: 661 AENKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWS 720
             NKN +YF+ +  P GV+VL+WPRIL+FS  +Q  SYY+T  P +   GRY FGEI W+
Sbjct: 681 GPNKNTVYFVDIIRPVGVEVLIWPRILVFSKCQQEHSYYVTFKPTEIFSGRYVFGEIMWT 740

BLAST of Cp4.1LG05g08730 vs. TAIR10
Match: AT4G10520.1 (AT4G10520.1 Subtilase family protein)

HSP 1 Score: 538.9 bits (1387), Expect = 4.8e-153
Identity = 309/718 (43.04%), Postives = 432/718 (60.17%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           ++YSY+  FSGF+AKL  SQA  +S++  V+ +  +   E+ TTR+WD+LG+  P  S S
Sbjct: 67  IVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDS 126

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
                    K   G +V+VG+ DSG+WPESE F +D+   P+P  WKG C     FN S+
Sbjct: 127 LL------QKANMGYNVIVGVIDSGVWPESEMF-NDKGFGPIPSRWKGGCESGELFNASI 186

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CNRKLIGA+Y++ G  AE+G +N + NPE+ SPRDF GHGTH AST  G  + +VS+ G
Sbjct: 187 HCNRKLIGAKYFVDGLVAEFGVVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVG 246

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSP 240
             LG+G ARGGAP   +AVYK CW     G C+ ADV+ A D+A+ DGVD++S S G S 
Sbjct: 247 --LGRGTARGGAPGVHIAVYKACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSV 306

Query: 241 PLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQ 300
           PL P  E +S +G+FH + KG+ VV +AGN GP    + NV+PW + VAA+T DR+FPT 
Sbjct: 307 PLFPETEHTS-VGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTA 366

Query: 301 ILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVG 360
           I + N I+I+G+++     +           + G CE+ S      +  GKVV+CF+   
Sbjct: 367 ITLGNNITILGQAIYGGPELGFVGLTYPESPLSGDCEKLSANP-NSTMEGKVVLCFAAST 426

Query: 361 PVSMGDAQEALDAINAS--ALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
           P +   A      INA    LI    PT  L      P V ID    T I  F     R 
Sbjct: 427 PSNAAIAA----VINAGGLGLIMAKNPTHSLTPTRKFPWVSIDFELGTDIL-FYIRSTRS 486

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+V+I+A++++ G+SV+  VA FSSRGP+S+SP ILKPDI+APGVNILAA  P +     
Sbjct: 487 PIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNS----- 546

Query: 481 PTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDST 540
                  S     +   SGTSM+ P +SGVV L+KSLHP WSP+AI+SA++TTA + D +
Sbjct: 547 -------SINDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPS 606

Query: 541 RNTILAGGST-KPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMI 600
              I A GS+ K +DPFD GGG +NP KAV PGL+YDMT +DY++++C++ Y++  I  +
Sbjct: 607 GEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRV 666

Query: 601 VNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPP 660
           +    +  VC  P    S+ +LN PSIT+ NL+   T+ RTV NV    N++Y + ++PP
Sbjct: 667 LG---KITVCPNP--KPSVLDLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPP 726

Query: 661 NGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 716
            G+ V V P  L+F +     S+ + ++   K    Y FG + W+D  H V  P+ VR
Sbjct: 727 TGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVR 745

BLAST of Cp4.1LG05g08730 vs. TAIR10
Match: AT1G32940.1 (AT1G32940.1 Subtilase family protein)

HSP 1 Score: 529.6 bits (1363), Expect = 2.9e-150
Identity = 311/728 (42.72%), Postives = 437/728 (60.03%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           M+YSY+  FSGF+AKL  SQA  L+    V+ +      EL TTR+WD+LGL + + +  
Sbjct: 67  MVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPNNL 126

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
              DT        GD V++G  D+G+WPESESF D+  + P+P  WKG C    +F  S 
Sbjct: 127 LN-DTNM------GDQVIIGFIDTGVWPESESFNDNG-VGPIPSHWKGGCESGEKFI-ST 186

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CNRKLIGA+Y++ GF AE  G NT+ + ++ S RDF+GHGTHTAS A G  V ++S+ G
Sbjct: 187 NCNRKLIGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKG 246

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYEG--KCTDADVMAAFDDALCDGVDVISASFGG 240
             L  G  RGGAPRAR+A+YK CW  D  G   C+ +D++ A D+++ DGVDV+S S G 
Sbjct: 247 --LAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGA 306

Query: 241 SPPLAP--FFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRT 300
             PL P        A G+FH + KG+ VV + GN GP    V N +PW I VAA+T+DR+
Sbjct: 307 QIPLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRS 366

Query: 301 FPTQILIQNRISIMGESLIT------TNIINAKLANAINYFIDGVCERRSIRKGRKSGAG 360
           FPT I + NR  I+G++L T      T+++  + A   N    GVCER ++   R + AG
Sbjct: 367 FPTPITLGNRKVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNLNPNR-TMAG 426

Query: 361 KVVVCFST-VGPVSMGDAQEALDAINASALIFGAPPTLQL-PDLDLLPTVRIDITHATQI 420
           KVV+CF+T     ++  A   + A     +I    P   L P  D  P V ID    T +
Sbjct: 427 KVVLCFTTNTLFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDV 486

Query: 421 RNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAA 480
             ++    R PVV+I+ +R+++G+ V   VA FSSRGP+S+SP ILKPDI APGV+ILAA
Sbjct: 487 LLYIRS-TRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAA 546

Query: 481 WPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSAL 540
             P++  +V              ++  +GTSM+ P ++GVVAL+K+LHP+WSPAA RSA+
Sbjct: 547 TSPDSNSSVG------------GFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAI 606

Query: 541 ITTATKRDSTRNTILA-GGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNI 600
           +TTA + D     I A G S K +DPFD GGG VNP KA +PGL+YDM   DYI++LC+ 
Sbjct: 607 VTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSA 666

Query: 601 GYTEQQIRMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKN 660
           GY +  I  +V       VC  P   TS+ ++N PSIT+ +LK   T+ RTV NV    +
Sbjct: 667 GYNDSSITQLVG---NVTVCSTP--KTSVLDVNLPSITIPDLKDEVTLTRTVTNVG-TVD 726

Query: 661 AIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHR 716
           ++Y + + PP G+QV+V P  L+F+   ++VS+ + ++   K    + FG + W+D  H 
Sbjct: 727 SVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHN 763

BLAST of Cp4.1LG05g08730 vs. TAIR10
Match: AT1G32960.1 (AT1G32960.1 Subtilase family protein)

HSP 1 Score: 507.3 bits (1305), Expect = 1.6e-143
Identity = 308/728 (42.31%), Postives = 423/728 (58.10%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           M+YSY+  FSGF+AKL  SQA  ++ +  V+ +      EL TTR+W++LGL     S +
Sbjct: 70  MVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGL-----SSA 129

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
              +    + +  GD V++G+ D+G+WPESESF D+  + P+P  WKG C     F  S 
Sbjct: 130 NPKNLLNDTNM--GDQVIIGVIDTGVWPESESFNDNG-VGPIPRKWKGGCESGENFR-ST 189

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CNRKLIGA+Y++ GF AE  G NT+ + ++ S RDF GHGTH AS A G  V +VS+ G
Sbjct: 190 DCNRKLIGAKYFINGFLAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKG 249

Query: 181 SSLGKGIARGGAPRARLAVYKVCW-GKDYEG-KCTDADVMAAFDDALCDGVDVISASFGG 240
             L  G  RGGAPRAR+A+YK CW  ++ +G  C+D+D+M A D+A+ DGVDV+S S  G
Sbjct: 250 --LAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVG 309

Query: 241 SPPL--APFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRT 300
             PL          A G FH + KG+ VV + GNDGP    V N++PW + VAA+T+DR+
Sbjct: 310 QIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRS 369

Query: 301 FPTQILIQNRISIMGESLIT------TNIINAKLANAINYFIDGVCERRSIRKGRKSGAG 360
           FPT I + N   I+G++  T      T+++  + A   N    GVCE  ++     + A 
Sbjct: 370 FPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNP-NYTMAM 429

Query: 361 KVVVCFSTVGP-VSMGDAQEALDAINASALIFGAPPTLQL-PDLDLLPTVRIDITHATQI 420
           KVV+CF+      ++  A   + A     LI    P   L P  D  P V +D    T I
Sbjct: 430 KVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDI 489

Query: 421 RNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAA 480
            +++    R PVV+I+ +R++ G+ V   V  FSSRGP+S+SP ILKPDI+APGV ILAA
Sbjct: 490 LSYIRS-TRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 549

Query: 481 WPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSAL 540
             P     V              +   SGTSM+ P ISGV+AL+K+LHP WSPAA RSA+
Sbjct: 550 TSPNDTLNVG------------GFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAI 609

Query: 541 ITTATKRDSTRNTILA-GGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNI 600
           +TTA + D     I A G S K SDPFD GGG VNP KA  PGL+YDM   DYI++LC+ 
Sbjct: 610 VTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSA 669

Query: 601 GYTEQQIRMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKN 660
           GY +  I  +V    Q  VC  P    S+ ++N PSIT+ NLK   T+ RTV NV    +
Sbjct: 670 GYNDSSISQLVG---QITVCSNP--KPSVLDVNLPSITIPNLKDEVTLTRTVTNVG-LVD 729

Query: 661 AIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHR 716
           ++Y + + PP GV+V+V P  L+F+     VS+ + ++   K    Y FG + W+D  H 
Sbjct: 730 SVYKVSVEPPLGVRVVVTPETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHN 766

BLAST of Cp4.1LG05g08730 vs. TAIR10
Match: AT4G10510.1 (AT4G10510.1 Subtilase family protein)

HSP 1 Score: 504.2 bits (1297), Expect = 1.3e-142
Identity = 306/728 (42.03%), Postives = 424/728 (58.24%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           M++S++  FSGF+AKL  SQA  ++ +  V+ +   +  +  TTR+WD+LGL       S
Sbjct: 60  MVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDYLGL-------S 119

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
            T      ++   G+ +++GI DSG+WPESE F D+  + PVP  WKG C     FN S 
Sbjct: 120 PTNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNE-IGPVPSHWKGGCESGEDFNSS- 179

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTG 180
            CN+KLIGA+Y++  F A +   N+S + +F SPR + GHGTH A+ A G  V + S+ G
Sbjct: 180 HCNKKLIGAKYFINAFLATHESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYKG 239

Query: 181 SSLGKGIARGGAPRARLAVYKVCWGKDYE-GKCTDADVMAAFDDALCDGVDVISASFGGS 240
             L  G  RGGAPRAR+AVYK CW  D +   C+ AD++ A D+A+ DGVDV+S S  G 
Sbjct: 240 --LAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSL-GF 299

Query: 241 PPLAPFFE--SSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTF 300
            PL P  +     A G+FH + KG++VV +AGN GP    V N +PW + VAA+T+DR+F
Sbjct: 300 EPLYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSF 359

Query: 301 PTQILIQNRISIMGESLIT------TNIINAKLANAINYFIDGVCERRSIRKGRKSGAGK 360
            T + + N   I+G+++ T      T+++  +     N    G CER  I   R + AGK
Sbjct: 360 VTPMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERLLINSNR-TMAGK 419

Query: 361 VVVCFSTVGPVSMGDAQEALDAINASAL---IFGAPPTLQLPDLDLLPTVRIDITHATQI 420
           VV+CF T  P S+   + A     A  L   I G P  +  P LD  P V +D    T I
Sbjct: 420 VVLCF-TESPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGTYI 479

Query: 421 RNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAA 480
             F       PVV+I+ +R++IG+ V   VA FSSRGP+ +S  ILKPDI+APGV+ILAA
Sbjct: 480 L-FYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILAA 539

Query: 481 WPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSAL 540
                       +T   +     + F SGTSM+ P ISG+VAL+K+LHP WSPAAIRSA+
Sbjct: 540 ------------TTTNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAI 599

Query: 541 ITTATKRDSTRNTILAGGS-TKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNI 600
           +TTA + D     I A GS  KP+DPFD GGG VNP KA  PGLVYD+   DY++++C++
Sbjct: 600 VTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSV 659

Query: 601 GYTEQQIRMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKN 660
           GY E  I  +V    +  VC  P    S+ + N PSIT+ NLK   T+ RT+ NV     
Sbjct: 660 GYNETSISQLVG---KGTVCSYP--KPSVLDFNLPSITIPNLKEEVTLPRTLTNVGP-LE 719

Query: 661 AIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHR 716
           ++Y + + PP G QV V P  L+F+   + VS+ ++++   K    Y FG + WSD  H 
Sbjct: 720 SVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHN 754

BLAST of Cp4.1LG05g08730 vs. NCBI nr
Match: gi|659071746|ref|XP_008461720.1| (PREDICTED: subtilisin-like protease isoform X2 [Cucumis melo])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 555/721 (76.98%), Postives = 622/721 (86.27%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSYK+SFSGFSAKLNASQA+ LSKME VIS+F+S+TMELHTTRSWDFLGLPIPSY+ +
Sbjct: 1   MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMELHTTRSWDFLGLPIPSYTNN 60

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
           R+   P         DVVVGIFDSG+WPES+SF+++  +  +PC+WKGKCVK YRFNP+ 
Sbjct: 61  RSTLFPLRLPSYGDHDVVVGIFDSGVWPESKSFDENEGIGRIPCNWKGKCVKGYRFNPAS 120

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSG-NPEFESPRDFLGHGTHTASTAVGGIVEDVSF- 180
           ACNRKLIGARYYL GFEA+YG LNT+  NPEF SPRDFLGHGTHTASTAVG +V +V+F 
Sbjct: 121 ACNRKLIGARYYLNGFEAQYGALNTTAENPEFRSPRDFLGHGTHTASTAVGAVVHNVAFP 180

Query: 181 TGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGG 240
           T S L KG ARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG 
Sbjct: 181 TSSLLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGE 240

Query: 241 SPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFP 300
           +PPL P FES+SAIGSFH MQ+GVSVVFSAGNDGP+ SLVQNVSPWSICVAASTMDRTFP
Sbjct: 241 TPPLTPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFP 300

Query: 301 TQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFST 360
           T I I N  SIMGESLITTNIIN KLA+AINYF DG+CER++IRKG KSG GKVVVCFST
Sbjct: 301 TPIFILNHFSIMGESLITTNIINVKLADAINYFNDGICERKNIRKGGKSGKGKVVVCFST 360

Query: 361 VGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
           VG VS+  AQEAL AINASALIFGAPPT +LPDLDL+PTVRIDI  ATQIRN LAELPRL
Sbjct: 361 VGQVSIATAQEALKAINASALIFGAPPTTELPDLDLIPTVRIDIDQATQIRNLLAELPRL 420

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+VEI  ARSVIGKSVAP+VAYFSSRGPSS+ PDILKPDISAPGVNILAAWPPETAPTVR
Sbjct: 421 PMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVR 480

Query: 481 PTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDST 540
           P+    E +  VKWNFQSGTSMSCPHISGVVALIKS+HP+WSPAAIRSA+ITTATK D++
Sbjct: 481 PSGKFNEEEEGVKWNFQSGTSMSCPHISGVVALIKSVHPNWSPAAIRSAIITTATKVDTS 540

Query: 541 RNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI-RMI 600
            NTILAGGS K SDPFDIG GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI  +I
Sbjct: 541 GNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQINNLI 600

Query: 601 VNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPP 660
           +NPSP    CC    +T IAN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL++ PP
Sbjct: 601 LNPSPH--FCCRQSTAT-IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLRVLPP 660

Query: 661 NGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVS 719
            GV+V VWPR+L FS FRQ +SYY+TITPL+K+RGRY FGEI+W + FH VTSPLVVR++
Sbjct: 661 YGVRVQVWPRVLFFSCFRQQISYYVTITPLRKSRGRYGFGEIQWFNRFHTVTSPLVVRLA 718

BLAST of Cp4.1LG05g08730 vs. NCBI nr
Match: gi|659071744|ref|XP_008461718.1| (PREDICTED: subtilisin-like protease isoform X1 [Cucumis melo])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 555/722 (76.87%), Postives = 622/722 (86.15%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSYK+SFSGFSAKLNASQA+ LSKME VIS+F+S+TMELHTTRSWDFLGLPIPSY+ +
Sbjct: 1   MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMELHTTRSWDFLGLPIPSYTNN 60

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
           R+   P         DVVVGIFDSG+WPES+SF+++  +  +PC+WKGKCVK YRFNP+ 
Sbjct: 61  RSTLFPLRLPSYGDHDVVVGIFDSGVWPESKSFDENEGIGRIPCNWKGKCVKGYRFNPAS 120

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSG-NPEFESPRDFLGHGTHTASTAVGGIVEDVSF- 180
           ACNRKLIGARYYL GFEA+YG LNT+  NPEF SPRDFLGHGTHTASTAVG +V +V+F 
Sbjct: 121 ACNRKLIGARYYLNGFEAQYGALNTTAENPEFRSPRDFLGHGTHTASTAVGAVVHNVAFP 180

Query: 181 TGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGG 240
           T S L KG ARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG 
Sbjct: 181 TSSLLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGE 240

Query: 241 SPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFP 300
           +PPL P FES+SAIGSFH MQ+GVSVVFSAGNDGP+ SLVQNVSPWSICVAASTMDRTFP
Sbjct: 241 TPPLTPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFP 300

Query: 301 TQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFST 360
           T I I N  SIMGESLITTNIIN KLA+AINYF DG+CER++IRKG KSG GKVVVCFST
Sbjct: 301 TPIFILNHFSIMGESLITTNIINVKLADAINYFNDGICERKNIRKGGKSGKGKVVVCFST 360

Query: 361 VGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
           VG VS+  AQEAL AINASALIFGAPPT +LPDLDL+PTVRIDI  ATQIRN LAELPRL
Sbjct: 361 VGQVSIATAQEALKAINASALIFGAPPTTELPDLDLIPTVRIDIDQATQIRNLLAELPRL 420

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+VEI  ARSVIGKSVAP+VAYFSSRGPSS+ PDILKPDISAPGVNILAAWPPETAPTVR
Sbjct: 421 PMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVR 480

Query: 481 PTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITT-ATKRDS 540
           P+    E +  VKWNFQSGTSMSCPHISGVVALIKS+HP+WSPAAIRSA+ITT ATK D+
Sbjct: 481 PSGKFNEEEEGVKWNFQSGTSMSCPHISGVVALIKSVHPNWSPAAIRSAIITTAATKVDT 540

Query: 541 TRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI-RM 600
           + NTILAGGS K SDPFDIG GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI  +
Sbjct: 541 SGNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQINNL 600

Query: 601 IVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNP 660
           I+NPSP    CC    +T IAN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL++ P
Sbjct: 601 ILNPSPH--FCCRQSTAT-IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLRVLP 660

Query: 661 PNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRV 719
           P GV+V VWPR+L FS FRQ +SYY+TITPL+K+RGRY FGEI+W + FH VTSPLVVR+
Sbjct: 661 PYGVRVQVWPRVLFFSCFRQQISYYVTITPLRKSRGRYGFGEIQWFNRFHTVTSPLVVRL 719

BLAST of Cp4.1LG05g08730 vs. NCBI nr
Match: gi|778664986|ref|XP_011648457.1| (PREDICTED: subtilisin-like protease SBT3.5 [Cucumis sativus])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 553/725 (76.28%), Postives = 618/725 (85.24%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSYK+SFSGFSAKLNASQA+ LSKME VIS+F+S+TM+LHTTRSWDFLGLPIPSY+ +
Sbjct: 84  MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMKLHTTRSWDFLGLPIPSYTNN 143

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
           R+             DVVV IFDSG+WPES+SFE+   +  +PC+WKGKCVK YRFNP+ 
Sbjct: 144 RSARFSLHLPSYGDHDVVVAIFDSGVWPESKSFEESEGIGRIPCNWKGKCVKGYRFNPAS 203

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSG-NPEFESPRDFLGHGTHTASTAVGGIVEDVSF- 180
           ACNRKLIGARYYLKGFEA+YG LNT+  NPEF SPRDFLGHGTHTASTAVG +V +V F 
Sbjct: 204 ACNRKLIGARYYLKGFEAQYGALNTTADNPEFRSPRDFLGHGTHTASTAVGAVVHNVGFP 263

Query: 181 TGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGG 240
           T SSL KG ARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG 
Sbjct: 264 TSSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGE 323

Query: 241 SPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFP 300
            PPL P FES+SAIGSFH MQ+GVSVVFSAGNDG + SLVQNVSPWSICVAASTMDRTFP
Sbjct: 324 RPPLIPLFESASAIGSFHAMQRGVSVVFSAGNDGSHPSLVQNVSPWSICVAASTMDRTFP 383

Query: 301 TQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFST 360
           T I I N  SIMGESLIT NIIN KLA+AINYF DG+CER +IRKG KSG GKVVVCFST
Sbjct: 384 TTIFILNHFSIMGESLITRNIINVKLADAINYFNDGICERENIRKGGKSGKGKVVVCFST 443

Query: 361 VGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
           +G VS+  AQEA+ AINASALIFGAPPT +LPDLDL+PTVRIDI  ATQIRNFLAELPRL
Sbjct: 444 IGQVSIATAQEAVKAINASALIFGAPPTTELPDLDLIPTVRIDIHQATQIRNFLAELPRL 503

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+VEI  ARSVIGKSVAP+VAYFSSRGPSS+ PDILKPDISAPGVNILAAWPPETAPTVR
Sbjct: 504 PMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVR 563

Query: 481 PT----STEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATK 540
           P+      E E +  VKWNFQSGTSMSCPH+SGVVALIKS+HP+WSPAAIRSA+ITTATK
Sbjct: 564 PSGKINEEEEEEEEGVKWNFQSGTSMSCPHVSGVVALIKSVHPNWSPAAIRSAIITTATK 623

Query: 541 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 600
            DS+ NTILAGGS K SDPFDIG GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI
Sbjct: 624 IDSSGNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQI 683

Query: 601 -RMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLK 660
             +I+NPSP    CC    +T IAN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL+
Sbjct: 684 SNLILNPSPH--FCCRQSTAT-IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLR 743

Query: 661 LNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLV 719
           + PP GV+V VWPR+L FS +RQ +SYYITITPL+K+RGRY FGEI+W + FH VTSPLV
Sbjct: 744 VLPPYGVRVQVWPRVLFFSCYRQQISYYITITPLRKSRGRYGFGEIQWFNRFHTVTSPLV 803

BLAST of Cp4.1LG05g08730 vs. NCBI nr
Match: gi|700209811|gb|KGN64907.1| (hypothetical protein Csa_1G153520 [Cucumis sativus])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 553/725 (76.28%), Postives = 618/725 (85.24%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSYK+SFSGFSAKLNASQA+ LSKME VIS+F+S+TM+LHTTRSWDFLGLPIPSY+ +
Sbjct: 21  MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMKLHTTRSWDFLGLPIPSYTNN 80

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
           R+             DVVV IFDSG+WPES+SFE+   +  +PC+WKGKCVK YRFNP+ 
Sbjct: 81  RSARFSLHLPSYGDHDVVVAIFDSGVWPESKSFEESEGIGRIPCNWKGKCVKGYRFNPAS 140

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSG-NPEFESPRDFLGHGTHTASTAVGGIVEDVSF- 180
           ACNRKLIGARYYLKGFEA+YG LNT+  NPEF SPRDFLGHGTHTASTAVG +V +V F 
Sbjct: 141 ACNRKLIGARYYLKGFEAQYGALNTTADNPEFRSPRDFLGHGTHTASTAVGAVVHNVGFP 200

Query: 181 TGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGG 240
           T SSL KG ARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG 
Sbjct: 201 TSSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGE 260

Query: 241 SPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFP 300
            PPL P FES+SAIGSFH MQ+GVSVVFSAGNDG + SLVQNVSPWSICVAASTMDRTFP
Sbjct: 261 RPPLIPLFESASAIGSFHAMQRGVSVVFSAGNDGSHPSLVQNVSPWSICVAASTMDRTFP 320

Query: 301 TQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFST 360
           T I I N  SIMGESLIT NIIN KLA+AINYF DG+CER +IRKG KSG GKVVVCFST
Sbjct: 321 TTIFILNHFSIMGESLITRNIINVKLADAINYFNDGICERENIRKGGKSGKGKVVVCFST 380

Query: 361 VGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRL 420
           +G VS+  AQEA+ AINASALIFGAPPT +LPDLDL+PTVRIDI  ATQIRNFLAELPRL
Sbjct: 381 IGQVSIATAQEAVKAINASALIFGAPPTTELPDLDLIPTVRIDIHQATQIRNFLAELPRL 440

Query: 421 PVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVR 480
           P+VEI  ARSVIGKSVAP+VAYFSSRGPSS+ PDILKPDISAPGVNILAAWPPETAPTVR
Sbjct: 441 PMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVR 500

Query: 481 PT----STEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATK 540
           P+      E E +  VKWNFQSGTSMSCPH+SGVVALIKS+HP+WSPAAIRSA+ITTATK
Sbjct: 501 PSGKINEEEEEEEEGVKWNFQSGTSMSCPHVSGVVALIKSVHPNWSPAAIRSAIITTATK 560

Query: 541 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 600
            DS+ NTILAGGS K SDPFDIG GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI
Sbjct: 561 IDSSGNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQI 620

Query: 601 -RMIVNPSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLK 660
             +I+NPSP    CC    +T IAN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL+
Sbjct: 621 SNLILNPSPH--FCCRQSTAT-IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLR 680

Query: 661 LNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLV 719
           + PP GV+V VWPR+L FS +RQ +SYYITITPL+K+RGRY FGEI+W + FH VTSPLV
Sbjct: 681 VLPPYGVRVQVWPRVLFFSCYRQQISYYITITPLRKSRGRYGFGEIQWFNRFHTVTSPLV 740

BLAST of Cp4.1LG05g08730 vs. NCBI nr
Match: gi|764567503|ref|XP_011462200.1| (PREDICTED: subtilisin-like protease SBT3.5, partial [Fragaria vesca subsp. vesca])

HSP 1 Score: 904.4 bits (2336), Expect = 1.2e-259
Identity = 451/720 (62.64%), Postives = 567/720 (78.75%), Query Frame = 1

Query: 1   MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 60
           MLYSYK SFSGFSAK+N+SQA+TL+K+EGVIS+F+SKT++LHTTRSWDFLGLP+ S S S
Sbjct: 51  MLYSYKHSFSGFSAKINSSQAVTLAKVEGVISVFRSKTVQLHTTRSWDFLGLPMIS-STS 110

Query: 61  RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 120
              D     +LA G DV+VG+FD+GIWPESESF ++  M P+P +WKG+CVK   F P+ 
Sbjct: 111 TASDAASPLQLAYGQDVIVGVFDTGIWPESESFREEPHMRPIPSTWKGECVKGEMFEPAK 170

Query: 121 ACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIV-EDVSFT 180
           ACNRKLIGARYYL+GFE EYG LNTSGNPEF S RDFLGHGTHTASTAVG +V ++ SF 
Sbjct: 171 ACNRKLIGARYYLEGFEEEYGPLNTSGNPEFRSARDFLGHGTHTASTAVGSVVKQNASFY 230

Query: 181 GSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGS 240
           G    +G ARGGAP+ARLAVYKVCWGK+Y  +CT AD++AAFDDAL DGV VISAS G  
Sbjct: 231 G--FAEGTARGGAPKARLAVYKVCWGKNYSSRCTGADILAAFDDALHDGVHVISASIGSR 290

Query: 241 PPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPT 300
           PPL P F S  AIGSFH MQ GVSVVFSAGN+GP  S V NV+PWS+CVAAS++DR FPT
Sbjct: 291 PPLIPLFASEGAIGSFHAMQLGVSVVFSAGNEGPDPSQVTNVAPWSLCVAASSIDRMFPT 350

Query: 301 QILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTV 360
           ++L+ N++SIMGESLITT  I AKLA+A  YF +G+C  R+ +K  K  A  V++CFST+
Sbjct: 351 RVLLDNKLSIMGESLITTP-IKAKLADASVYFYNGICTSRNWKKS-KYAANIVIICFSTI 410

Query: 361 GPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLP 420
           GP  +G+A+ A    NAS LIF  P + ++   D++P+V ++I   T+I N+L+E P  P
Sbjct: 411 GPYEIGEAEVAAKLANASGLIFVEPMSREIGP-DIIPSVHVNIEQGTRILNYLSESPTKP 470

Query: 421 VVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETAPTVRP 480
           VV+I A++++IGK+ AP VAYFSSRGP+SL+PDILKPDISAPG+NILAAWP  T+PT+R 
Sbjct: 471 VVQIEASKTIIGKAPAPRVAYFSSRGPNSLTPDILKPDISAPGINILAAWPNRTSPTLR- 530

Query: 481 TSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDSTR 540
                +  R V WNFQSGTSMSCPH+SG+VALIKS HP WSPAAI+SA++TTA  +D++ 
Sbjct: 531 ----RDDNRLVNWNFQSGTSMSCPHVSGIVALIKSSHPDWSPAAIKSAIMTTAYTKDTSF 590

Query: 541 NTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVN 600
           +TILA G  K SDPFD+G G ++P+KA++PGLVYDM   DY+ FLCNIGYTE+QI MIV 
Sbjct: 591 DTILADGPMKASDPFDLGAGHIDPIKAMDPGLVYDMKTRDYVQFLCNIGYTEEQINMIV- 650

Query: 601 PSPQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPPNG 660
           P P       P +  S +N+NYPSIT++NL+ST TIKRT+RNV ENKNA+YF  ++ P+G
Sbjct: 651 PCPSGTDTSCPRVPKSNSNVNYPSITVSNLQSTVTIKRTMRNVGENKNAVYFGTISEPDG 710

Query: 661 VQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSA 720
           V+V++WPR+L+FSWF+   +YY+T+ P KK++GRYDFGEI WSDGFH+V SPLVV V++A
Sbjct: 711 VEVVIWPRVLVFSWFKAENTYYVTLKPQKKSQGRYDFGEIVWSDGFHKVRSPLVVCVNTA 758

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT3I_ARATH6.0e-23857.95Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana GN=SBT3.18 PE=3 SV=2[more]
SBT39_ARATH8.6e-15243.04Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana GN=SBT3.9 PE=3 SV=1[more]
SBT35_ARATH5.2e-14942.72Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana GN=SBT3.5 PE=1 SV=1[more]
SBT3A_ARATH3.2e-14641.92Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana GN=SBT3.10 PE=3 SV=2[more]
SBT33_ARATH2.8e-14242.31Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana GN=SBT3.3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVP7_CUCSA0.0e+0076.28Uncharacterized protein OS=Cucumis sativus GN=Csa_1G153520 PE=4 SV=1[more]
A0A067EU78_CITSI1.6e-25862.22Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g003970mg PE=4 SV=1[more]
M5WNJ5_PRUPE3.4e-25661.75Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa017229mg PE=4 S... [more]
A0A061GYM5_THECC7.1e-25461.30Subtilisin-like serine endopeptidase family protein OS=Theobroma cacao GN=TCM_03... [more]
V4TYX3_9ROSI1.5e-24861.96Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014478mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G26330.12.7e-23656.85 Subtilisin-like serine endopeptidase family protein[more]
AT4G10520.14.8e-15343.04 Subtilase family protein[more]
AT1G32940.12.9e-15042.72 Subtilase family protein[more]
AT1G32960.11.6e-14342.31 Subtilase family protein[more]
AT4G10510.11.3e-14242.03 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|659071746|ref|XP_008461720.1|0.0e+0076.98PREDICTED: subtilisin-like protease isoform X2 [Cucumis melo][more]
gi|659071744|ref|XP_008461718.1|0.0e+0076.87PREDICTED: subtilisin-like protease isoform X1 [Cucumis melo][more]
gi|778664986|ref|XP_011648457.1|0.0e+0076.28PREDICTED: subtilisin-like protease SBT3.5 [Cucumis sativus][more]
gi|700209811|gb|KGN64907.1|0.0e+0076.28hypothetical protein Csa_1G153520 [Cucumis sativus][more]
gi|764567503|ref|XP_011462200.1|1.2e-25962.64PREDICTED: subtilisin-like protease SBT3.5, partial [Fragaria vesca subsp. vesca... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR023828Peptidase_S8_Ser-AS
IPR015500Peptidase_S8_subtilisin-rel
IPR010259S8pro/Inhibitor_I9
IPR000209Peptidase_S8/S53_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009567 double fertilization forming a zygote and endosperm
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g08730.1Cp4.1LG05g08730.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 18..95
score: 2.2E-77coord: 135..293
score: 2.2E-77coord: 435..571
score: 2.2
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 74..534
score: 5.1
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 122..332
score: 1.02E-75coord: 42..94
score: 1.02E-75coord: 438..571
score: 1.02
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 1..42
score: 4.
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 156..169
score: 3.7E-13coord: 496..512
score: 3.7E-13coord: 74..93
score: 3.7
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..720
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 497..507
scor
NoneNo IPR availablePANTHERPTHR10795:SF352SUBTILISIN PROTEASE-LIKEcoord: 1..720
score:

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG05g08730CmaCh02G008620Cucurbita maxima (Rimu)cmacpeB646
Cp4.1LG05g08730CmoCh02G008620Cucurbita moschata (Rifu)cmocpeB595
Cp4.1LG05g08730Carg20247Silver-seed gourdcarcpeB0429
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG05g08730Cucumber (Chinese Long) v3cpecucB0907
Cp4.1LG05g08730Cucumber (Chinese Long) v3cpecucB0924
Cp4.1LG05g08730Wax gourdcpewgoB0907
Cp4.1LG05g08730Wax gourdcpewgoB0943
Cp4.1LG05g08730Cucurbita pepo (Zucchini)cpecpeB308
Cp4.1LG05g08730Cucurbita pepo (Zucchini)cpecpeB401
Cp4.1LG05g08730Cucurbita pepo (Zucchini)cpecpeB402
Cp4.1LG05g08730Cucurbita pepo (Zucchini)cpecpeB458
Cp4.1LG05g08730Cucumber (Gy14) v1cgycpeB0460
Cp4.1LG05g08730Cucurbita maxima (Rimu)cmacpeB500
Cp4.1LG05g08730Cucurbita maxima (Rimu)cmacpeB584
Cp4.1LG05g08730Cucurbita maxima (Rimu)cmacpeB751
Cp4.1LG05g08730Cucurbita maxima (Rimu)cmacpeB752
Cp4.1LG05g08730Cucurbita moschata (Rifu)cmocpeB459
Cp4.1LG05g08730Cucurbita moschata (Rifu)cmocpeB533
Cp4.1LG05g08730Cucurbita moschata (Rifu)cmocpeB705
Cp4.1LG05g08730Cucurbita moschata (Rifu)cmocpeB706
Cp4.1LG05g08730Wild cucumber (PI 183967)cpecpiB727
Cp4.1LG05g08730Wild cucumber (PI 183967)cpecpiB747
Cp4.1LG05g08730Cucumber (Chinese Long) v2cpecuB726
Cp4.1LG05g08730Cucumber (Chinese Long) v2cpecuB734
Cp4.1LG05g08730Cucumber (Chinese Long) v2cpecuB745
Cp4.1LG05g08730Bottle gourd (USVL1VR-Ls)cpelsiB615
Cp4.1LG05g08730Bottle gourd (USVL1VR-Ls)cpelsiB588
Cp4.1LG05g08730Bottle gourd (USVL1VR-Ls)cpelsiB614
Cp4.1LG05g08730Watermelon (Charleston Gray)cpewcgB632
Cp4.1LG05g08730Watermelon (Charleston Gray)cpewcgB658
Cp4.1LG05g08730Watermelon (97103) v1cpewmB690
Cp4.1LG05g08730Watermelon (97103) v1cpewmB744
Cp4.1LG05g08730Melon (DHL92) v3.5.1cpemeB654
Cp4.1LG05g08730Melon (DHL92) v3.5.1cpemeB668
Cp4.1LG05g08730Melon (DHL92) v3.5.1cpemeB688
Cp4.1LG05g08730Cucumber (Gy14) v2cgybcpeB138
Cp4.1LG05g08730Cucumber (Gy14) v2cgybcpeB434
Cp4.1LG05g08730Cucumber (Gy14) v2cgybcpeB560
Cp4.1LG05g08730Cucumber (Gy14) v2cgybcpeB848
Cp4.1LG05g08730Melon (DHL92) v3.6.1cpemedB798
Cp4.1LG05g08730Melon (DHL92) v3.6.1cpemedB820
Cp4.1LG05g08730Silver-seed gourdcarcpeB0475
Cp4.1LG05g08730Silver-seed gourdcarcpeB0624