Carg20247 (gene) Silver-seed gourd

BLAST of Carg20247 vs. NCBI nr
Match: XP_022947798.1 (subtilisin-like protease SBT3.18 [Cucurbita moschata])

HSP 1 Score: 1449.9 bits (3752), Expect = 0.0e+00
Identity = 746/774 (96.38%), Postives = 755/774 (97.55%), Query Frame = 0

Query: 10  HITSVEEGNETFFITRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYK 69
           H+TS+         + VYIVYLGHTRLEDATLTSESHLRLLSKVF+SEEDGKRAMLYSYK
Sbjct: 16  HLTSIS--------SHVYIVYLGHTRLEDATLTSESHLRLLSKVFSSEEDGKRAMLYSYK 75

Query: 70  RSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTP 129
            SFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSG RTFDTP
Sbjct: 76  HSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGRRTFDTP 135

Query: 130 RSRKLADGADVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKL 189
           RSRKLADG DVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKL
Sbjct: 136 RSRKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKL 195

Query: 190 IGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKG 249
           IGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVG IVEDVSFTGSSLGKG
Sbjct: 196 IGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTGSSLGKG 255

Query: 250 IARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFF 309
           IARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFF
Sbjct: 256 IARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFF 315

Query: 310 ESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNR 369
           ESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNV PWSICVAASTMDRTFPTQILIQNR
Sbjct: 316 ESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTFPTQILIQNR 375

Query: 370 ISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGD 429
           ISIMGESLITTNIINAKLANAINYFIDGVCERRSIRK RKSGAGKVVVCFST GPVSMGD
Sbjct: 376 ISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVGPVSMGD 435

Query: 430 AQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAA 489
           AQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVV+IRAA
Sbjct: 436 AQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVQIRAA 495

Query: 490 RSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGES 549
           RSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPT+TEGES
Sbjct: 496 RSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTATEGES 555

Query: 550 QRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGG 609
           QRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSAL+TTATK+DSTRNTILAGG
Sbjct: 556 QRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDSTRNTILAGG 615

Query: 610 STKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLV 669
           STKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPS QTLV
Sbjct: 616 STKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSSQTLV 675

Query: 670 CCPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWP 729
           CCPPFLSTS+ANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWP
Sbjct: 676 CCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWP 735

Query: 730 RILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS 784
           RILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS
Sbjct: 736 RILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS 781

BLAST of Carg20247 vs. NCBI nr
Match: XP_023533046.1 (subtilisin-like protease SBT3.18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 748/777 (96.27%), Postives = 757/777 (97.43%), Query Frame = 0

Query: 10  HITSVEEGNETFFITRVYIVYLGHTRLEDATLTSESHLRLLSKVFA---SEEDGKRAMLY 69
           H+TS+         + VYIVYLGHTRLEDATLTSESHLRLLSKVF+   SEEDGKRAMLY
Sbjct: 16  HLTSIS--------SHVYIVYLGHTRLEDATLTSESHLRLLSKVFSSPCSEEDGKRAMLY 75

Query: 70  SYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTF 129
           SYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTF
Sbjct: 76  SYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTF 135

Query: 130 DTPRSRKLADGADVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACN 189
           DTPRS KLADG DVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACN
Sbjct: 136 DTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACN 195

Query: 190 RKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSL 249
           RKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVG IVEDVSFTGSSL
Sbjct: 196 RKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTGSSL 255

Query: 250 GKGIARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLA 309
           GKGIARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLA
Sbjct: 256 GKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLA 315

Query: 310 PFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILI 369
           PFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILI
Sbjct: 316 PFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILI 375

Query: 370 QNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVS 429
           QNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRK RKSGAGKVVVCFST GPVS
Sbjct: 376 QNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVGPVS 435

Query: 430 MGDAQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEI 489
           MGDAQ+AL+AINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEI
Sbjct: 436 MGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEI 495

Query: 490 RAARSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTE 549
           RAARSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTE
Sbjct: 496 RAARSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTE 555

Query: 550 GESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTIL 609
           GESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSAL+TTATK+DSTRNTIL
Sbjct: 556 GESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATKRDSTRNTIL 615

Query: 610 AGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQ 669
           AGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQ
Sbjct: 616 AGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQ 675

Query: 670 TLVCCPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVL 729
           TLVCCPPFLSTS+ANLNYPSITLANLKSTTTIKRTVRNVA NKNAIYFLKLNPPNGVQVL
Sbjct: 676 TLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYFLKLNPPNGVQVL 735

Query: 730 VWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS 784
           VWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS
Sbjct: 736 VWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS 784

BLAST of Carg20247 vs. NCBI nr
Match: XP_023007061.1 (subtilisin-like protease SBT3.18 [Cucurbita maxima])

HSP 1 Score: 1426.0 bits (3690), Expect = 0.0e+00
Identity = 734/774 (94.83%), Postives = 747/774 (96.51%), Query Frame = 0

Query: 10  HITSVEEGNETFFITRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYK 69
           H+TS+         + VYIVYLGHT LEDATLTSESHLRLLSKVFASEEDGKRAMLYSYK
Sbjct: 16  HLTSIS--------SHVYIVYLGHTHLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYK 75

Query: 70  RSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTP 129
           RSFSGFSAKLNASQAMTLSKMEGVISIF S+TMELHTTRSWDFLGLPIPSYSGSRT DTP
Sbjct: 76  RSFSGFSAKLNASQAMTLSKMEGVISIFMSRTMELHTTRSWDFLGLPIPSYSGSRTPDTP 135

Query: 130 RSRKLADGADVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKL 189
           RSRKLADG DVVVGIFDSGIWPESESFEDD+WM PVPCSWKGKCVKAYRFNP+LACNRKL
Sbjct: 136 RSRKLADGDDVVVGIFDSGIWPESESFEDDQWMFPVPCSWKGKCVKAYRFNPALACNRKL 195

Query: 190 IGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKG 249
           IGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVG IVEDVSFTGSSLGKG
Sbjct: 196 IGARYYLKGFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGGIVEDVSFTGSSLGKG 255

Query: 250 IARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFF 309
           IARGGAP ARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPF 
Sbjct: 256 IARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFI 315

Query: 310 ESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNR 369
           ESSSAIGSFH MQKGVSVVFSAGNDGPYRSLVQNV PWSICVAASTMDRTFPTQILIQNR
Sbjct: 316 ESSSAIGSFHAMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTFPTQILIQNR 375

Query: 370 ISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGD 429
           ISI+GESLITTNIINAKLANAINYFIDGVCERRSIRK RKSGAGKVVVCFST GPVSMGD
Sbjct: 376 ISIVGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVVCFSTVGPVSMGD 435

Query: 430 AQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAA 489
           AQ+AL AINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAA
Sbjct: 436 AQEALKAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAA 495

Query: 490 RSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGES 549
           RSVIGKS APSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGES
Sbjct: 496 RSVIGKSAAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGES 555

Query: 550 QRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGG 609
           QRQVKWNFQSGTSMSCPHISGVVAL+KSLHPSWSPAAIRSAL+TTATK+DSTRNTILAGG
Sbjct: 556 QRQVKWNFQSGTSMSCPHISGVVALVKSLHPSWSPAAIRSALITTATKRDSTRNTILAGG 615

Query: 610 STKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLV 669
           STKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMI+NPS QTLV
Sbjct: 616 STKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIMNPSQQTLV 675

Query: 670 CCPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWP 729
           CCPP LSTS+ANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWP
Sbjct: 676 CCPPILSTSIANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWP 735

Query: 730 RILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSAS 784
           RILLFSWFRQHVSYYITITPLKKA GRYDFGEIEWSDGFHRVTSPLVVRVSSAS
Sbjct: 736 RILLFSWFRQHVSYYITITPLKKAHGRYDFGEIEWSDGFHRVTSPLVVRVSSAS 781

BLAST of Carg20247 vs. NCBI nr
Match: XP_008461718.2 (PREDICTED: subtilisin-like protease SBT3.18 [Cucumis melo])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 571/763 (74.84%), Postives = 643/763 (84.27%), Query Frame = 0

Query: 24  TRVYIVYLGHTRL-EDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNAS 83
           +RVYIVYLGH  L +DATLTS+ HLRLLSKVFASEEDGKRAMLYSYK+SFSGFSAKLNAS
Sbjct: 29  SRVYIVYLGHNHLNDDATLTSKYHLRLLSKVFASEEDGKRAMLYSYKKSFSGFSAKLNAS 88

Query: 84  QAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVV 143
           QA+ LSKME VIS+F+S+TMELHTTRSWDFLGLPIPSY+ +R+   P         DVVV
Sbjct: 89  QAIALSKMEDVISVFESRTMELHTTRSWDFLGLPIPSYTNNRSTLFPLRLPSYGDHDVVV 148

Query: 144 GIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAE 203
           GIFDSG+WPES+SF+++  +  +PC+WKGKCVK YRFNP+ ACNRKLIGARYYL GFEA+
Sbjct: 149 GIFDSGVWPESKSFDENEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLNGFEAQ 208

Query: 204 YGGLNTSG-NPEFESPRDFLGHGTHTASTAVGAIVEDVSF-TGSSLGKGIARGGAPWARL 263
           YG LNT+  NPEF SPRDFLGHGTHTASTAVGA+V +V+F T S L KG ARGGAPWARL
Sbjct: 209 YGALNTTAENPEFRSPRDFLGHGTHTASTAVGAVVHNVAFPTSSLLAKGTARGGAPWARL 268

Query: 264 AVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHG 323
           AVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG +PPL P FES+SAIGSFH 
Sbjct: 269 AVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGETPPLTPLFESASAIGSFHA 328

Query: 324 MQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITT 383
           MQ+GVSVVFSAGNDGP+ SLVQNVSPWSICVAASTMDRTFPT I I N  SIMGESLITT
Sbjct: 329 MQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIFILNHFSIMGESLITT 388

Query: 384 NIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINAS 443
           NIIN KLA+AINYF DG+CER++IRK  KSG GKVVVCFST G VS+  AQ+AL AINAS
Sbjct: 389 NIINVKLADAINYFNDGICERKNIRKGGKSGKGKVVVCFSTVGQVSIATAQEALKAINAS 448

Query: 444 ALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPS 503
           ALIFGAPPT +LPDLDL+PTVRIDI  ATQIRN LAELPRLP+VEI  ARSVIGKSVAP+
Sbjct: 449 ALIFGAPPTTELPDLDLIPTVRIDIDQATQIRNLLAELPRLPMVEIGVARSVIGKSVAPT 508

Query: 504 VAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSG 563
           VAYFSSRGP                 NILAAWPPETAPTVRP+    E +  VKWNFQSG
Sbjct: 509 VAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVRPSGKFNEEEEGVKWNFQSG 568

Query: 564 TSMSCPHISGVVALIKSLHPSWSPAAIRSALVTT-ATKKDSTRNTILAGGSTKPSDPFDI 623
           TSMSCPHISGVVALIKS+HP+WSPAAIRSA++TT ATK D++ NTILAGGS K SDPFDI
Sbjct: 569 TSMSCPHISGVVALIKSVHPNWSPAAIRSAIITTAATKVDTSGNTILAGGSMKASDPFDI 628

Query: 624 GGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI-RMIVNPSPQTLVCCPPFLSTS 683
           G GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI  +I+NPSP    CC    +T 
Sbjct: 629 GAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQINNLILNPSPH--FCCRQSTAT- 688

Query: 684 VANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFR 743
           +AN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL++ PP GV+V VWPR+L FS FR
Sbjct: 689 IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLRVLPPYGVRVQVWPRVLFFSCFR 748

Query: 744 QHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 782
           Q +SYY+TITPL+K+RGRY FGEI+W + FH VTSPLVVR+++
Sbjct: 749 QQISYYVTITPLRKSRGRYGFGEIQWFNRFHTVTSPLVVRLAT 788

BLAST of Carg20247 vs. NCBI nr
Match: XP_011648457.1 (PREDICTED: subtilisin-like protease SBT3.5 [Cucumis sativus])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 568/766 (74.15%), Postives = 636/766 (83.03%), Query Frame = 0

Query: 24  TRVYIVYLGHTRL-EDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNAS 83
           + VYIVYLGH RL +DATLTS+ HL LLSKVFASEEDGKRAMLYSYK+SFSGFSAKLNAS
Sbjct: 43  SHVYIVYLGHNRLNDDATLTSKYHLHLLSKVFASEEDGKRAMLYSYKKSFSGFSAKLNAS 102

Query: 84  QAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVV 143
           QA+ LSKME VIS+F+S+TM+LHTTRSWDFLGLPIPSY+ +R+             DVVV
Sbjct: 103 QAIALSKMEDVISVFESRTMKLHTTRSWDFLGLPIPSYTNNRSARFSLHLPSYGDHDVVV 162

Query: 144 GIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAE 203
            IFDSG+WPES+SFE+   +  +PC+WKGKCVK YRFNP+ ACNRKLIGARYYLKGFEA+
Sbjct: 163 AIFDSGVWPESKSFEESEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQ 222

Query: 204 YGGLNTSG-NPEFESPRDFLGHGTHTASTAVGAIVEDVSF-TGSSLGKGIARGGAPWARL 263
           YG LNT+  NPEF SPRDFLGHGTHTASTAVGA+V +V F T SSL KG ARGGAPWARL
Sbjct: 223 YGALNTTADNPEFRSPRDFLGHGTHTASTAVGAVVHNVGFPTSSSLAKGTARGGAPWARL 282

Query: 264 AVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHG 323
           AVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG  PPL P FES+SAIGSFH 
Sbjct: 283 AVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGERPPLIPLFESASAIGSFHA 342

Query: 324 MQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITT 383
           MQ+GVSVVFSAGNDG + SLVQNVSPWSICVAASTMDRTFPT I I N  SIMGESLIT 
Sbjct: 343 MQRGVSVVFSAGNDGSHPSLVQNVSPWSICVAASTMDRTFPTTIFILNHFSIMGESLITR 402

Query: 384 NIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINAS 443
           NIIN KLA+AINYF DG+CER +IRK  KSG GKVVVCFST G VS+  AQ+A+ AINAS
Sbjct: 403 NIINVKLADAINYFNDGICERENIRKGGKSGKGKVVVCFSTIGQVSIATAQEAVKAINAS 462

Query: 444 ALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPS 503
           ALIFGAPPT +LPDLDL+PTVRIDI  ATQIRNFLAELPRLP+VEI  ARSVIGKSVAP+
Sbjct: 463 ALIFGAPPTTELPDLDLIPTVRIDIHQATQIRNFLAELPRLPMVEIGVARSVIGKSVAPT 522

Query: 504 VAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQ----VKWN 563
           VAYFSSRGP                 NILAAWPPETAPTVRP+    E        VKWN
Sbjct: 523 VAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVRPSGKINEXXXXXXXGVKWN 582

Query: 564 FQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGSTKPSDP 623
           FQSGTSMSCPH+SGVVALIKS+HP+WSPAAIRSA++TTATK DS+ NTILAGGS K SDP
Sbjct: 583 FQSGTSMSCPHVSGVVALIKSVHPNWSPAAIRSAIITTATKIDSSGNTILAGGSMKASDP 642

Query: 624 FDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI-RMIVNPSPQTLVCCPPFL 683
           FDIG GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI  +I+NPSP    CC    
Sbjct: 643 FDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQISNLILNPSPH--FCCRQST 702

Query: 684 STSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFS 743
           +T +AN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL++ PP GV+V VWPR+L FS
Sbjct: 703 AT-IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLRVLPPYGVRVQVWPRVLFFS 762

Query: 744 WFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 782
            +RQ +SYYITITPL+K+RGRY FGEI+W + FH VTSPLVVR+++
Sbjct: 763 CYRQQISYYITITPLRKSRGRYGFGEIQWFNRFHTVTSPLVVRLAT 805

BLAST of Carg20247 vs. TAIR10
Match: AT4G26330.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 785.4 bits (2027), Expect = 3.2e-227
Identity = 422/745 (56.64%), Postives = 554/745 (74.36%), Query Frame = 0

Query: 56  SEEDGKRAMLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGL 115
           S++D +++MLYSY   F GFSAKLN++QA +L+K+  VI++FKSK+++LHTTRSWDFLGL
Sbjct: 13  SKDDAEQSMLYSYNNGFLGFSAKLNSTQAASLAKLNQVITVFKSKSLKLHTTRSWDFLGL 72

Query: 116 PIPSYSGSRTFDTPRSRKLADGADVVVGIFDS--------------GIWPESESFEDDRW 175
            +     +R    P   +LA G+D+VVGIFD+              GIWPESESF +   
Sbjct: 73  AV---DNARRTPPP---QLAYGSDIVVGIFDTGLFISLKLLLLSILGIWPESESFRETPE 132

Query: 176 MSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEYGGLNTSGNPEFESPRDFL 235
             P+P SW GKCV    F+PS+ CNRKLIGAR+YL+GFE  YG ++ + +PE+ SPRD+L
Sbjct: 133 AKPIPSSWNGKCVGGEDFDPSVHCNRKLIGARFYLRGFEETYGTIDFTRDPEYRSPRDYL 192

Query: 236 GHGTHTASTAVGAIVEDVS-FTGSSLGKGIARGGAPWARLAVYKVCWGKDYEGKCTDADV 295
           GHGTHTASTAVG++V +VS F G  LG+G ARGGAP ARLAV+K CWGKD EG CT+AD+
Sbjct: 193 GHGTHTASTAVGSVVRNVSGFFG--LGRGTARGGAPLARLAVFKTCWGKDLEGVCTEADI 252

Query: 296 MAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSL 355
           +AAFDDA+ DGV VISASFG SPPL+PFFESS+ IG+FH  ++G+SVVFS GNDGP   +
Sbjct: 253 LAAFDDAIHDGVHVISASFGYSPPLSPFFESSADIGAFHAAERGISVVFSTGNDGPDPGV 312

Query: 356 VQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCE 415
           VQNV+PW++ VAAST+DR+FPT+I+I    ++ G+SLI+   I   LA A  YF  GVC+
Sbjct: 313 VQNVAPWAVSVAASTVDRSFPTRIVIDGSFTLTGQSLISQE-ITGTLALATTYFNGGVCK 372

Query: 416 RRSIRKDRKSGAGKVVVCFSTAGPVS-MGDAQQALNAINASALIFGAPPTLQL-PDLDLL 475
             +  K  K     +++CFST GPV  + +AQ A    NA ALIF A PT QL  ++D++
Sbjct: 373 WENWMK--KLANETIILCFSTLGPVQFIEEAQAAAIRANALALIFAASPTRQLAEEVDMI 432

Query: 476 PTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPXXXXXXXXX 535
           PTVR+DI H T+IRN+LA  P +P+V+I  +++VIG++ APSVAYFSSRGPXXXXXXXXX
Sbjct: 433 PTVRVDILHGTRIRNYLARSPTVPMVKIGPSKTVIGETTAPSVAYFSSRGPXXXXXXXXX 492

Query: 536 XXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSL 595
           XXXXXXXX ILAAWPP T PT+ P        R ++WNFQSGTSMSCPH++GV+AL++S 
Sbjct: 493 XXXXXXXXGILAAWPPRTPPTLLP-----GDHRSIEWNFQSGTSMSCPHVAGVMALLQSA 552

Query: 596 HPSWSPAAIRSALVTTATKKDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDM 655
           HP WSP+AIRSA++TTA  +D++ + IL+GGS K +DPFDIG G +NPLKA++PGLVY+ 
Sbjct: 553 HPDWSPSAIRSAIMTTAYTRDTSYDLILSGGSMKSTDPFDIGAGHINPLKAMDPGLVYNT 612

Query: 656 TANDYIIFLCNIGYTEQQIR-MIVNPSPQTLVCCPPFLSTSVANLNYPSITLANLKSTTT 715
             +DY++F+CNIGYT+Q+I+ M+++P P T  C P     + A+ NYPSIT+ +L+ T T
Sbjct: 613 RTDDYVLFMCNIGYTDQEIKSMVLHPEPST-TCLPSHSYRTNADFNYPSITIPSLRLTRT 672

Query: 716 IKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRY 775
           IKRTV NV  NKN +YF+ +  P GV+VL+WPRIL+FS  +Q  SYY+T  P +   GRY
Sbjct: 673 IKRTVSNVGPNKNTVYFVDIIRPVGVEVLIWPRILVFSKCQQEHSYYVTFKPTEIFSGRY 732

Query: 776 DFGEIEWSDGFHRVTSPLVVRVSSA 783
            FGEI W++G HRV SP+VV +S+A
Sbjct: 733 VFGEIMWTNGLHRVRSPVVVFLSNA 740

BLAST of Carg20247 vs. TAIR10
Match: AT4G10520.1 (Subtilase family protein)

HSP 1 Score: 533.1 bits (1372), Expect = 2.9e-151
Identity = 309/756 (40.87%), Postives = 443/756 (58.60%), Query Frame = 0

Query: 24  TRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQ 83
           ++VY+VYLG    ++    +ESH ++L  +  S+E    +++YSY+  FSGF+AKL  SQ
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQ 86

Query: 84  AMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVG 143
           A  +S++  V+ +  +   E+ TTR+WD+LG+  P  S S        +K   G +V+VG
Sbjct: 87  AQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDSLL------QKANMGYNVIVG 146

Query: 144 IFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEY 203
           + DSG+WPESE F +D+   P+P  WKG C     FN S+ CNRKLIGA+Y++ G  AE+
Sbjct: 147 VIDSGVWPESEMF-NDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEF 206

Query: 204 GGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAVY 263
           G +N + NPE+ SPRDF GHGTH AST  G+ + +VS+ G  LG+G ARGGAP   +AVY
Sbjct: 207 GVVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVG--LGRGTARGGAPGVHIAVY 266

Query: 264 KVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQK 323
           K CW     G C+ ADV+ A D+A+ DGVD++S S G S PL P  E +S +G+FH + K
Sbjct: 267 KACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEHTS-VGAFHAVAK 326

Query: 324 GVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTNII 383
           G+ VV +AGN GP    + NV+PW + VAA+T DR+FPT I + N I+I+G+++     +
Sbjct: 327 GIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPEL 386

Query: 384 NAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINASALI 443
                      + G CE+ S   +  +  GKVV+CF+ + P +   A   +NA     LI
Sbjct: 387 GFVGLTYPESPLSGDCEKLSANPN-STMEGKVVLCFAASTPSNAAIA-AVINA-GGLGLI 446

Query: 444 FGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVAY 503
               PT  L      P V ID    T I  F     R P+V+I+A++++ G+SV+  VA 
Sbjct: 447 MAKNPTHSLTPTRKFPWVSIDFELGTDIL-FYIRSTRSPIVKIQASKTLFGQSVSTKVAT 506

Query: 504 FSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGTSM 563
           FSSRGP                 NILAA  P +            S     +   SGTSM
Sbjct: 507 FSSRGPNSVSPAILKPDIAAPGVNILAAISPNS------------SINDGGFAMMSGTSM 566

Query: 564 SCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGST-KPSDPFDIGGG 623
           + P +SGVV L+KSLHP WSP+AI+SA+VTTA + D +   I A GS+ K +DPFD GGG
Sbjct: 567 ATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGG 626

Query: 624 QVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVCCPPFLSTSVANL 683
            +NP KAV PGL+YDMT +DY++++C++ Y++  I  ++    +  VC  P    SV +L
Sbjct: 627 LINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLG---KITVCPNP--KPSVLDL 686

Query: 684 NYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHVS 743
           N PSIT+ NL+   T+ RTV NV    N++Y + ++PP G+ V V P  L+F +     S
Sbjct: 687 NLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRS 745

Query: 744 YYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
           + + ++   K    Y FG + W+D  H V  P+ VR
Sbjct: 747 FTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVR 745

BLAST of Carg20247 vs. TAIR10
Match: AT1G32940.1 (Subtilase family protein)

HSP 1 Score: 524.6 bits (1350), Expect = 1.0e-148
Identity = 313/768 (40.76%), Postives = 447/768 (58.20%), Query Frame = 0

Query: 24  TRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQ 83
           ++V+IVYLG  + +D    SESH ++LS +  S+ D   +M+YSY+  FSGF+AKL  SQ
Sbjct: 27  SKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLTESQ 86

Query: 84  AMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVG 143
           A  L+    V+ +      EL TTR+WD+LGL + +   +   DT        G  V++G
Sbjct: 87  AKKLADSPEVVHVMADSFYELATTRTWDYLGLSVAN-PNNLLNDTNM------GDQVIIG 146

Query: 144 IFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEY 203
             D+G+WPESESF +D  + P+P  WKG C    +F  S  CNRKLIGA+Y++ GF AE 
Sbjct: 147 FIDTGVWPESESF-NDNGVGPIPSHWKGGCESGEKF-ISTNCNRKLIGAKYFINGFLAEN 206

Query: 204 GGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAVY 263
            G NT+ + ++ S RDF+GHGTHTAS A G+ V ++S+ G  L  G  RGGAP AR+A+Y
Sbjct: 207 EGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKG--LAGGNLRGGAPRARIAIY 266

Query: 264 KVCWGKDYEG--KCTDADVMAAFDDALCDGVDVISASFGGSPPLAP--FFESSSAIGSFH 323
           K CW  D  G   C+ +D++ A D+++ DGVDV+S S G   PL P        A G+FH
Sbjct: 267 KACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFH 326

Query: 324 GMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLIT 383
            + KG+ VV + GN GP    V N +PW I VAA+T+DR+FPT I + NR  I+G++L T
Sbjct: 327 AVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYT 386

Query: 384 ------TNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGP-VSMGDAQQ 443
                 T+++  + A   N    GVCER ++  +R + AGKVV+CF+T     ++  A  
Sbjct: 387 GQELGFTSLVYPENAGFTNETFSGVCERLNLNPNR-TMAGKVVLCFTTNTLFTAVSRAAS 446

Query: 444 ALNAINASALIFGAPPTLQL-PDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARS 503
            + A     +I    P   L P  D  P V ID    T +  ++    R PVV+I+ +R+
Sbjct: 447 YVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRS-TRSPVVKIQPSRT 506

Query: 504 VIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQR 563
           ++G+ V   VA FSSRGP                 +ILAA  P++  +V           
Sbjct: 507 LVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVG---------- 566

Query: 564 QVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILA-GGS 623
              ++  +GTSM+ P ++GVVAL+K+LHP+WSPAA RSA+VTTA + D     I A G S
Sbjct: 567 --GFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSS 626

Query: 624 TKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVC 683
            K +DPFD GGG VNP KA +PGL+YDM   DYI++LC+ GY +  I  +V       VC
Sbjct: 627 RKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVG---NVTVC 686

Query: 684 CPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPR 743
             P   TSV ++N PSIT+ +LK   T+ RTV NV    +++Y + + PP G+QV+V P 
Sbjct: 687 STP--KTSVLDVNLPSITIPDLKDEVTLTRTVTNV-GTVDSVYKVVVEPPLGIQVVVAPE 746

Query: 744 ILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
            L+F+   ++VS+ + ++   K    + FG + W+D  H VT P+ VR
Sbjct: 747 TLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVR 763

BLAST of Carg20247 vs. TAIR10
Match: AT4G10510.1 (Subtilase family protein)

HSP 1 Score: 502.7 bits (1293), Expect = 4.2e-142
Identity = 311/769 (40.44%), Postives = 433/769 (56.31%), Query Frame = 0

Query: 23  ITRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNAS 82
           I  V+IVYLG  + +D    +ESH R+L  +  S+E+   +M++S++  FSGF+AKL  S
Sbjct: 19  IIYVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTES 78

Query: 83  QAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVV 142
           QA  ++ +  V+ +   +  +  TTR+WD+LGL       S T       +   G  +++
Sbjct: 79  QAKKIADLPEVVHVIPDRFYKPATTRTWDYLGL-------SPTNPKNLLNQTNMGEQMII 138

Query: 143 GIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAE 202
           GI DSG+WPESE F D+  + PVP  WKG C     FN S  CN+KLIGA+Y++  F A 
Sbjct: 139 GIIDSGVWPESEVFNDNE-IGPVPSHWKGGCESGEDFNSS-HCNKKLIGAKYFINAFLAT 198

Query: 203 YGGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAV 262
           +   N+S + +F SPR + GHGTH A+ A G+ V + S+ G  L  G  RGGAP AR+AV
Sbjct: 199 HESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYKG--LAGGTVRGGAPRARIAV 258

Query: 263 YKVCWGKDYE-GKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFE--SSSAIGSFH 322
           YK CW  D +   C+ AD++ A D+A+ DGVDV+S S  G  PL P  +     A G+FH
Sbjct: 259 YKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSL-GFEPLYPETDVRDGIATGAFH 318

Query: 323 GMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLIT 382
            + KG++VV +AGN GP    V N +PW + VAA+T+DR+F T + + N   I+G+++ T
Sbjct: 319 AVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYT 378

Query: 383 ------TNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQA 442
                 T+++  +     N    G CER  I  +R + AGKVV+CF T  P S+   + A
Sbjct: 379 GTEVGFTSLVYPENPGNSNESFSGTCERLLINSNR-TMAGKVVLCF-TESPYSISVTRAA 438

Query: 443 LNAINASAL---IFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAAR 502
                A  L   I G P  +  P LD  P V +D    T I  F       PVV+I+ +R
Sbjct: 439 HYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGTYIL-FYIRSNGSPVVKIQPSR 498

Query: 503 SVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQ 562
           ++IG+ V   VA FSSRGP                 +ILAA            +T   + 
Sbjct: 499 TLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILAA------------TTTNTTF 558

Query: 563 RQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGS 622
               + F SGTSM+ P ISG+VAL+K+LHP WSPAAIRSA+VTTA + D     I A GS
Sbjct: 559 NDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGS 618

Query: 623 -TKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLV 682
             KP+DPFD GGG VNP KA  PGLVYD+   DY++++C++GY E  I  +V    +  V
Sbjct: 619 PRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG---KGTV 678

Query: 683 CCPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWP 742
           C  P    SV + N PSIT+ NLK   T+ RT+ NV     ++Y + + PP G QV V P
Sbjct: 679 CSYP--KPSVLDFNLPSITIPNLKEEVTLPRTLTNVGP-LESVYRVAVEPPLGTQVTVTP 738

Query: 743 RILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
             L+F+   + VS+ ++++   K    Y FG + WSD  H VT PL VR
Sbjct: 739 ETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVR 754

BLAST of Carg20247 vs. TAIR10
Match: AT4G10530.1 (Subtilase family protein)

HSP 1 Score: 502.3 bits (1292), Expect = 5.4e-142
Identity = 299/761 (39.29%), Postives = 438/761 (57.56%), Query Frame = 0

Query: 24  TRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQ 83
           ++VY+VYLG    ++    +ESH ++L  +  S+E    +++YSY+  FSGF+AKL  SQ
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQ 86

Query: 84  AMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVG 143
           A  +S++  V+ +  +   E+ TTR+WD+LG+  P  S S        +K   G +V+VG
Sbjct: 87  AQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDSLL------QKANMGYNVIVG 146

Query: 144 IFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEY 203
           + D+G+WPESE F +D+   P+P  WKG C     FN S+ CNRKLIGA+Y++    A++
Sbjct: 147 VIDTGVWPESEMF-NDKGYGPIPSRWKGGCESGELFNGSIHCNRKLIGAKYFIDANNAQF 206

Query: 204 GGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAVY 263
           G LN + NP++ SPRDF GHGTH AST  G+ + +VS+ G  LG+G ARGGAP   +AVY
Sbjct: 207 GVLNKTENPDYLSPRDFNGHGTHVASTIGGSFLPNVSYLG--LGRGTARGGAPGVHIAVY 266

Query: 264 KVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESS--SAIGSFHGM 323
           K CW    +  C+ ADV+ A D+A+ DGVD++S S   S PL P  ++   +++G+FH +
Sbjct: 267 KACW---VQRGCSGADVLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAV 326

Query: 324 QKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTN 383
            KG+ VV +A N GP    + NV+PW + VAA+T DR+FPT I + N I+I+G+++   +
Sbjct: 327 AKGIPVVAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIFGGS 386

Query: 384 IINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINASA 443
            +           + G CE+ S    + +  GKVV+CF+ + P S       +NA     
Sbjct: 387 ELGFVGLTYPESPLSGDCEKLS-ANPKSAMEGKVVLCFAASTP-SNAAITAVINA-GGLG 446

Query: 444 LIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSV 503
           LI    PT  L  L   P V +D    T I  F     R P+V I+A+R++ G+SV+  V
Sbjct: 447 LIMARNPTHLLRPLRNFPYVSVDFELGTDIL-FYIRSTRSPIVNIQASRTLFGQSVSTKV 506

Query: 504 AYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVK---WNFQ 563
           A FSSRGP                             +V P   +   Q  +    +   
Sbjct: 507 ATFSSRGPN----------------------------SVSPAILKLFLQIAINDGGFAMM 566

Query: 564 SGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGST-KPSDPF 623
           SGTSM+ P +SGVV L+KSLHP WSP+AI+SA+VTTA + D +   I A GS+ K +DPF
Sbjct: 567 SGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPF 626

Query: 624 DIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVCCPPFLST 683
           D GGG +NP KAV PGL+YDMT +DY++++C++ Y++  I  ++    +  VC  P    
Sbjct: 627 DYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLG---KITVCPNP--KP 686

Query: 684 SVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWF 743
           SV +LN PSIT+ NL+   T+ RTV NV    N++Y + ++PP GV V V P  L+F   
Sbjct: 687 SVLDLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPPTGVNVAVTPTELVFDST 736

Query: 744 RQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
               S+ + ++   K    Y FG + W+D  H V  P+ VR
Sbjct: 747 TTKRSFTVRVSTTHKVNTGYYFGSLTWTDTLHNVAIPVSVR 736

BLAST of Carg20247 vs. Swiss-Prot
Match: sp|Q9STQ2|SBT3I_ARATH (Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana OX=3702 GN=SBT3.18 PE=3 SV=2)

HSP 1 Score: 830.9 bits (2145), Expect = 1.2e-239
Identity = 441/761 (57.95%), Postives = 576/761 (75.69%), Query Frame = 0

Query: 26  VYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQAM 85
           VY+VYLG  RL++A L S  HL LLSKVF S++D +++MLYSY   F GFSAKLN++QA 
Sbjct: 30  VYVVYLGANRLKNAALASSHHLHLLSKVFTSKDDAEQSMLYSYNNGFLGFSAKLNSTQAA 89

Query: 86  TLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVGIF 145
           +L+K+  VI++FKSK+++LHTTRSWDFLGL +     +R    P   +LA G+D+VVGIF
Sbjct: 90  SLAKLNQVITVFKSKSLKLHTTRSWDFLGLAV---DNARRTPPP---QLAYGSDIVVGIF 149

Query: 146 DSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEYGG 205
           D+GIWPESESF +     P+P SW GKCV    F+PS+ CNRKLIGAR+YL+GFE  YG 
Sbjct: 150 DTGIWPESESFRETPEAKPIPSSWNGKCVGGEDFDPSVHCNRKLIGARFYLRGFEETYGT 209

Query: 206 LNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVS-FTGSSLGKGIARGGAPWARLAVYK 265
           ++ + +PE+ SPRD+LGHGTHTASTAVG++V +VS F G  LG+G ARGGAP ARLAV+K
Sbjct: 210 IDFTRDPEYRSPRDYLGHGTHTASTAVGSVVRNVSGFFG--LGRGTARGGAPLARLAVFK 269

Query: 266 VCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQKG 325
            CWGKD EG CT+AD++AAFDDA+ DGV VISASFG SPPL+PFFESS+ IG+FH  ++G
Sbjct: 270 TCWGKDLEGVCTEADILAAFDDAIHDGVHVISASFGYSPPLSPFFESSADIGAFHAAERG 329

Query: 326 VSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTNIIN 385
           +SVVFS GNDGP   +VQNV+PW++ VAAST+DR+FPT+I+I    ++ G+SLI+   I 
Sbjct: 330 ISVVFSTGNDGPDPGVVQNVAPWAVSVAASTVDRSFPTRIVIDGSFTLTGQSLISQE-IT 389

Query: 386 AKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVS-MGDAQQALNAINASALI 445
             LA A  YF  GVC+  +  K  K     +++CFST GPV  + +AQ A    NA ALI
Sbjct: 390 GTLALATTYFNGGVCKWENWMK--KLANETIILCFSTLGPVQFIEEAQAAAIRANALALI 449

Query: 446 FGAPPTLQL-PDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVA 505
           F A PT QL  ++D++PTVR+DI H T+IRN+LA  P +P+V+I  +++VIG++ APSVA
Sbjct: 450 FAASPTRQLAEEVDMIPTVRVDILHGTRIRNYLARSPTVPMVKIGPSKTVIGETTAPSVA 509

Query: 506 YFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGTS 565
           YFSSRGPXXXXXXXXXXXXXXXXX ILAAWPP T PT+ P        R ++WNFQSGTS
Sbjct: 510 YFSSRGPXXXXXXXXXXXXXXXXXGILAAWPPRTPPTLLP-----GDHRSIEWNFQSGTS 569

Query: 566 MSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGSTKPSDPFDIGGG 625
           MSCPH++GV+AL++S HP WSP+AIRSA++TTA  +D++ + IL+GGS K +DPFDIG G
Sbjct: 570 MSCPHVAGVMALLQSAHPDWSPSAIRSAIMTTAYTRDTSYDLILSGGSMKSTDPFDIGAG 629

Query: 626 QVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIR-MIVNPSPQTLVCCPPFLSTSVAN 685
            +NPLKA++PGLVY+   +DY++F+CNIGYT+Q+I+ M+++P P T  C P     + A+
Sbjct: 630 HINPLKAMDPGLVYNTRTDDYVLFMCNIGYTDQEIKSMVLHPEPST-TCLPSHSYRTNAD 689

Query: 686 LNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHV 745
            NYPSIT+ +L+ T TIKRTV NV  NKN +YF+ +  P GV+VL+WPRIL+FS  +Q  
Sbjct: 690 FNYPSITIPSLRLTRTIKRTVSNVGPNKNTVYFVDIIRPVGVEVLIWPRILVFSKCQQEH 749

Query: 746 SYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSSA 783
           SYY+T  P +   GRY FGEI W++G HRV SP+VV +S+A
Sbjct: 750 SYYVTFKPTEIFSGRYVFGEIMWTNGLHRVRSPVVVFLSNA 773

BLAST of Carg20247 vs. Swiss-Prot
Match: sp|Q9ZSB0|SBT39_ARATH (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 5.2e-150
Identity = 309/756 (40.87%), Postives = 443/756 (58.60%), Query Frame = 0

Query: 24  TRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQ 83
           ++VY+VYLG    ++    +ESH ++L  +  S+E    +++YSY+  FSGF+AKL  SQ
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQ 86

Query: 84  AMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVG 143
           A  +S++  V+ +  +   E+ TTR+WD+LG+  P  S S        +K   G +V+VG
Sbjct: 87  AQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDSLL------QKANMGYNVIVG 146

Query: 144 IFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEY 203
           + DSG+WPESE F +D+   P+P  WKG C     FN S+ CNRKLIGA+Y++ G  AE+
Sbjct: 147 VIDSGVWPESEMF-NDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEF 206

Query: 204 GGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAVY 263
           G +N + NPE+ SPRDF GHGTH AST  G+ + +VS+ G  LG+G ARGGAP   +AVY
Sbjct: 207 GVVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVG--LGRGTARGGAPGVHIAVY 266

Query: 264 KVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQK 323
           K CW     G C+ ADV+ A D+A+ DGVD++S S G S PL P  E +S +G+FH + K
Sbjct: 267 KACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEHTS-VGAFHAVAK 326

Query: 324 GVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTNII 383
           G+ VV +AGN GP    + NV+PW + VAA+T DR+FPT I + N I+I+G+++     +
Sbjct: 327 GIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPEL 386

Query: 384 NAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINASALI 443
                      + G CE+ S   +  +  GKVV+CF+ + P +   A   +NA     LI
Sbjct: 387 GFVGLTYPESPLSGDCEKLSANPN-STMEGKVVLCFAASTPSNAAIA-AVINA-GGLGLI 446

Query: 444 FGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVAY 503
               PT  L      P V ID    T I  F     R P+V+I+A++++ G+SV+  VA 
Sbjct: 447 MAKNPTHSLTPTRKFPWVSIDFELGTDIL-FYIRSTRSPIVKIQASKTLFGQSVSTKVAT 506

Query: 504 FSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGTSM 563
           FSSRGP                 NILAA  P +            S     +   SGTSM
Sbjct: 507 FSSRGPNSVSPAILKPDIAAPGVNILAAISPNS------------SINDGGFAMMSGTSM 566

Query: 564 SCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGST-KPSDPFDIGGG 623
           + P +SGVV L+KSLHP WSP+AI+SA+VTTA + D +   I A GS+ K +DPFD GGG
Sbjct: 567 ATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGG 626

Query: 624 QVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVCCPPFLSTSVANL 683
            +NP KAV PGL+YDMT +DY++++C++ Y++  I  ++    +  VC  P    SV +L
Sbjct: 627 LINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLG---KITVCPNP--KPSVLDL 686

Query: 684 NYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHVS 743
           N PSIT+ NL+   T+ RTV NV    N++Y + ++PP G+ V V P  L+F +     S
Sbjct: 687 NLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRS 745

Query: 744 YYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
           + + ++   K    Y FG + W+D  H V  P+ VR
Sbjct: 747 FTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVR 745

BLAST of Carg20247 vs. Swiss-Prot
Match: sp|Q9MAP7|SBT35_ARATH (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.8e-147
Identity = 313/768 (40.76%), Postives = 447/768 (58.20%), Query Frame = 0

Query: 24  TRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQ 83
           ++V+IVYLG  + +D    SESH ++LS +  S+ D   +M+YSY+  FSGF+AKL  SQ
Sbjct: 27  SKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLTESQ 86

Query: 84  AMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVG 143
           A  L+    V+ +      EL TTR+WD+LGL + +   +   DT        G  V++G
Sbjct: 87  AKKLADSPEVVHVMADSFYELATTRTWDYLGLSVAN-PNNLLNDTNM------GDQVIIG 146

Query: 144 IFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEY 203
             D+G+WPESESF +D  + P+P  WKG C    +F  S  CNRKLIGA+Y++ GF AE 
Sbjct: 147 FIDTGVWPESESF-NDNGVGPIPSHWKGGCESGEKF-ISTNCNRKLIGAKYFINGFLAEN 206

Query: 204 GGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAVY 263
            G NT+ + ++ S RDF+GHGTHTAS A G+ V ++S+ G  L  G  RGGAP AR+A+Y
Sbjct: 207 EGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKG--LAGGNLRGGAPRARIAIY 266

Query: 264 KVCWGKDYEG--KCTDADVMAAFDDALCDGVDVISASFGGSPPLAP--FFESSSAIGSFH 323
           K CW  D  G   C+ +D++ A D+++ DGVDV+S S G   PL P        A G+FH
Sbjct: 267 KACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFH 326

Query: 324 GMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLIT 383
            + KG+ VV + GN GP    V N +PW I VAA+T+DR+FPT I + NR  I+G++L T
Sbjct: 327 AVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYT 386

Query: 384 ------TNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGP-VSMGDAQQ 443
                 T+++  + A   N    GVCER ++  +R + AGKVV+CF+T     ++  A  
Sbjct: 387 GQELGFTSLVYPENAGFTNETFSGVCERLNLNPNR-TMAGKVVLCFTTNTLFTAVSRAAS 446

Query: 444 ALNAINASALIFGAPPTLQL-PDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARS 503
            + A     +I    P   L P  D  P V ID    T +  ++    R PVV+I+ +R+
Sbjct: 447 YVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRS-TRSPVVKIQPSRT 506

Query: 504 VIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQR 563
           ++G+ V   VA FSSRGP                 +ILAA  P++  +V           
Sbjct: 507 LVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVG---------- 566

Query: 564 QVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILA-GGS 623
              ++  +GTSM+ P ++GVVAL+K+LHP+WSPAA RSA+VTTA + D     I A G S
Sbjct: 567 --GFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSS 626

Query: 624 TKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVC 683
            K +DPFD GGG VNP KA +PGL+YDM   DYI++LC+ GY +  I  +V       VC
Sbjct: 627 RKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVG---NVTVC 686

Query: 684 CPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPR 743
             P   TSV ++N PSIT+ +LK   T+ RTV NV    +++Y + + PP G+QV+V P 
Sbjct: 687 STP--KTSVLDVNLPSITIPDLKDEVTLTRTVTNV-GTVDSVYKVVVEPPLGIQVVVAPE 746

Query: 744 ILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
            L+F+   ++VS+ + ++   K    + FG + W+D  H VT P+ VR
Sbjct: 747 TLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVR 763

BLAST of Carg20247 vs. Swiss-Prot
Match: sp|Q9ZSB1|SBT3A_ARATH (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 514.2 bits (1323), Expect = 2.5e-144
Identity = 303/758 (39.97%), Postives = 439/758 (57.92%), Query Frame = 0

Query: 24  TRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQ 83
           ++VY+VYLG    ++    +ESH ++L  +  S+E    +++YSY+  FSGF+AKL  SQ
Sbjct: 27  SKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQ 86

Query: 84  AMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVG 143
           A  +S++  V+ +  +   E+ TTR+WD+LG+  P  S S        +K   G +V+VG
Sbjct: 87  AQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS-PGNSDSLL------QKANMGYNVIVG 146

Query: 144 IFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEY 203
           + D+G+WPESE F +D+   P+P  WKG C     FN S+ CNRKLIGA+Y++    A++
Sbjct: 147 VIDTGVWPESEMF-NDKGYGPIPSRWKGGCESGELFNGSIHCNRKLIGAKYFIDANNAQF 206

Query: 204 GGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAVY 263
           G LN + NP++ SPRDF GHGTH AST  G+ + +VS+ G  LG+G ARGGAP   +AVY
Sbjct: 207 GVLNKTENPDYLSPRDFNGHGTHVASTIGGSFLPNVSYLG--LGRGTARGGAPGVHIAVY 266

Query: 264 KVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESS--SAIGSFHGM 323
           K CW    +  C+ ADV+ A D+A+ DGVD++S S   S PL P  ++   +++G+FH +
Sbjct: 267 KACW---VQRGCSGADVLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAV 326

Query: 324 QKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTN 383
            KG+ VV +A N GP    + NV+PW + VAA+T DR+FPT I + N I+I+G+++   +
Sbjct: 327 AKGIPVVAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIFGGS 386

Query: 384 IINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINASA 443
            +           + G CE+ S    + +  GKVV+CF+ + P S       +NA     
Sbjct: 387 ELGFVGLTYPESPLSGDCEKLS-ANPKSAMEGKVVLCFAASTP-SNAAITAVINA-GGLG 446

Query: 444 LIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSV 503
           LI    PT  L  L   P V +D    T I  F     R P+V I+A+R++ G+SV+  V
Sbjct: 447 LIMARNPTHLLRPLRNFPYVSVDFELGTDIL-FYIRSTRSPIVNIQASRTLFGQSVSTKV 506

Query: 504 AYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSGT 563
           A FSSRGP                 NILAA  P              S     +   SGT
Sbjct: 507 ATFSSRGPNSVSPAILKPDIAAPGVNILAAISP-------------NSINDGGFAMMSGT 566

Query: 564 SMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGST-KPSDPFDIG 623
           SM+ P +SGVV L+KSLHP WSP+AI+SA+VTTA + D +   I A GS+ K +DPFD G
Sbjct: 567 SMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYG 626

Query: 624 GGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVCCPPFLSTSVA 683
           GG +NP KAV PGL+YDMT +DY++++C++ Y++  I  ++    +  VC  P    SV 
Sbjct: 627 GGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLG---KITVCPNP--KPSVL 686

Query: 684 NLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQH 743
           +LN PSIT+ NL+   T+ RTV NV    N++Y + ++PP GV V V P  L+F      
Sbjct: 687 DLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPPTGVNVAVTPTELVFDSTTTK 746

Query: 744 VSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
            S+ + ++   K    Y FG + W+D  H V  P+ VR
Sbjct: 747 RSFTVRVSTTHKVNTGYYFGSLTWTDTLHNVAIPVSVR 748

BLAST of Carg20247 vs. Swiss-Prot
Match: sp|Q9SZY2|SBT37_ARATH (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 503.4 bits (1295), Expect = 4.4e-141
Identity = 310/768 (40.36%), Postives = 434/768 (56.51%), Query Frame = 0

Query: 24  TRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNASQ 83
           ++V+IVYLG  + +D    +ESH R+L  +  S+E+   +M++S++  FSGF+AKL  SQ
Sbjct: 32  SKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQ 91

Query: 84  AMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVG 143
           A  ++ +  V+ +   +  +  TTR+WD+LGL       S T       +   G  +++G
Sbjct: 92  AKKIADLPEVVHVIPDRFYKPATTRTWDYLGL-------SPTNPKNLLNQTNMGEQMIIG 151

Query: 144 IFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEY 203
           I DSG+WPESE F D+  + PVP  WKG C     FN S  CN+KLIGA+Y++  F A +
Sbjct: 152 IIDSGVWPESEVFNDNE-IGPVPSHWKGGCESGEDFNSS-HCNKKLIGAKYFINAFLATH 211

Query: 204 GGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLAVY 263
              N+S + +F SPR + GHGTH A+ A G+ V + S+ G  L  G  RGGAP AR+AVY
Sbjct: 212 ESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYKG--LAGGTVRGGAPRARIAVY 271

Query: 264 KVCWGKDYE-GKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFE--SSSAIGSFHG 323
           K CW  D +   C+ AD++ A D+A+ DGVDV+S S  G  PL P  +     A G+FH 
Sbjct: 272 KTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSL-GFEPLYPETDVRDGIATGAFHA 331

Query: 324 MQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLIT- 383
           + KG++VV +AGN GP    V N +PW + VAA+T+DR+F T + + N   I+G+++ T 
Sbjct: 332 VLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTG 391

Query: 384 -----TNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQAL 443
                T+++  +     N    G CER  I  +R + AGKVV+CF T  P S+   + A 
Sbjct: 392 TEVGFTSLVYPENPGNSNESFSGTCERLLINSNR-TMAGKVVLCF-TESPYSISVTRAAH 451

Query: 444 NAINASAL---IFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARS 503
               A  L   I G P  +  P LD  P V +D    T I  F       PVV+I+ +R+
Sbjct: 452 YVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGTYIL-FYIRSNGSPVVKIQPSRT 511

Query: 504 VIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQR 563
           +IG+ V   VA FSSRGP                 +ILAA            +T   +  
Sbjct: 512 LIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILAA------------TTTNTTFN 571

Query: 564 QVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGS- 623
              + F SGTSM+ P ISG+VAL+K+LHP WSPAAIRSA+VTTA + D     I A GS 
Sbjct: 572 DRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSP 631

Query: 624 TKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTLVC 683
            KP+DPFD GGG VNP KA  PGLVYD+   DY++++C++GY E  I  +V    +  VC
Sbjct: 632 RKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG---KGTVC 691

Query: 684 CPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPR 743
             P    SV + N PSIT+ NLK   T+ RT+ NV     ++Y + + PP G QV V P 
Sbjct: 692 SYP--KPSVLDFNLPSITIPNLKEEVTLPRTLTNVGP-LESVYRVAVEPPLGTQVTVTPE 751

Query: 744 ILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVR 779
            L+F+   + VS+ ++++   K    Y FG + WSD  H VT PL VR
Sbjct: 752 TLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVR 766

BLAST of Carg20247 vs. TrEMBL
Match: tr|A0A1S3CFU3|A0A1S3CFU3_CUCME (subtilisin-like protease SBT3.18 OS=Cucumis melo OX=3656 GN=LOC103500248 PE=4 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 571/763 (74.84%), Postives = 643/763 (84.27%), Query Frame = 0

Query: 24  TRVYIVYLGHTRL-EDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNAS 83
           +RVYIVYLGH  L +DATLTS+ HLRLLSKVFASEEDGKRAMLYSYK+SFSGFSAKLNAS
Sbjct: 29  SRVYIVYLGHNHLNDDATLTSKYHLRLLSKVFASEEDGKRAMLYSYKKSFSGFSAKLNAS 88

Query: 84  QAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVV 143
           QA+ LSKME VIS+F+S+TMELHTTRSWDFLGLPIPSY+ +R+   P         DVVV
Sbjct: 89  QAIALSKMEDVISVFESRTMELHTTRSWDFLGLPIPSYTNNRSTLFPLRLPSYGDHDVVV 148

Query: 144 GIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAE 203
           GIFDSG+WPES+SF+++  +  +PC+WKGKCVK YRFNP+ ACNRKLIGARYYL GFEA+
Sbjct: 149 GIFDSGVWPESKSFDENEGIGRIPCNWKGKCVKGYRFNPASACNRKLIGARYYLNGFEAQ 208

Query: 204 YGGLNTSG-NPEFESPRDFLGHGTHTASTAVGAIVEDVSF-TGSSLGKGIARGGAPWARL 263
           YG LNT+  NPEF SPRDFLGHGTHTASTAVGA+V +V+F T S L KG ARGGAPWARL
Sbjct: 209 YGALNTTAENPEFRSPRDFLGHGTHTASTAVGAVVHNVAFPTSSLLAKGTARGGAPWARL 268

Query: 264 AVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHG 323
           AVYKVCWGKDYEGKCTDADVMAAFDDAL DGVDVISASFG +PPL P FES+SAIGSFH 
Sbjct: 269 AVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASFGETPPLTPLFESASAIGSFHA 328

Query: 324 MQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITT 383
           MQ+GVSVVFSAGNDGP+ SLVQNVSPWSICVAASTMDRTFPT I I N  SIMGESLITT
Sbjct: 329 MQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRTFPTPIFILNHFSIMGESLITT 388

Query: 384 NIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINAS 443
           NIIN KLA+AINYF DG+CER++IRK  KSG GKVVVCFST G VS+  AQ+AL AINAS
Sbjct: 389 NIINVKLADAINYFNDGICERKNIRKGGKSGKGKVVVCFSTVGQVSIATAQEALKAINAS 448

Query: 444 ALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPS 503
           ALIFGAPPT +LPDLDL+PTVRIDI  ATQIRN LAELPRLP+VEI  ARSVIGKSVAP+
Sbjct: 449 ALIFGAPPTTELPDLDLIPTVRIDIDQATQIRNLLAELPRLPMVEIGVARSVIGKSVAPT 508

Query: 504 VAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSG 563
           VAYFSSRGP                 NILAAWPPETAPTVRP+    E +  VKWNFQSG
Sbjct: 509 VAYFSSRGPSSILPDILKPDISAPGVNILAAWPPETAPTVRPSGKFNEEEEGVKWNFQSG 568

Query: 564 TSMSCPHISGVVALIKSLHPSWSPAAIRSALVTT-ATKKDSTRNTILAGGSTKPSDPFDI 623
           TSMSCPHISGVVALIKS+HP+WSPAAIRSA++TT ATK D++ NTILAGGS K SDPFDI
Sbjct: 569 TSMSCPHISGVVALIKSVHPNWSPAAIRSAIITTAATKVDTSGNTILAGGSMKASDPFDI 628

Query: 624 GGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI-RMIVNPSPQTLVCCPPFLSTS 683
           G GQVNP+ A+NPGL+YD+T NDYI FLCNIGYT+QQI  +I+NPSP    CC    +T 
Sbjct: 629 GAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQQINNLILNPSPH--FCCRQSTAT- 688

Query: 684 VANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFR 743
           +AN NYPSITLANL+STTTI+R VRNV+ NKNAIYFL++ PP GV+V VWPR+L FS FR
Sbjct: 689 IANFNYPSITLANLRSTTTIRRIVRNVSLNKNAIYFLRVLPPYGVRVQVWPRVLFFSCFR 748

Query: 744 QHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 782
           Q +SYY+TITPL+K+RGRY FGEI+W + FH VTSPLVVR+++
Sbjct: 749 QQISYYVTITPLRKSRGRYGFGEIQWFNRFHTVTSPLVVRLAT 788

BLAST of Carg20247 vs. TrEMBL
Match: tr|A0A0A0LVP7|A0A0A0LVP7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G153520 PE=4 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 6.3e-310
Identity = 547/741 (73.82%), Postives = 612/741 (82.59%), Query Frame = 0

Query: 51  SKVFA---SEEDGKRAMLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTT 110
           SKV A   SEEDGKRAMLYSYK+SFSGFSAKLNASQA+ LSKME VIS+F+S+TM+LHTT
Sbjct: 5   SKVLAIACSEEDGKRAMLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMKLHTT 64

Query: 111 RSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVVGIFDSGIWPESESFEDDRWMSPVPC 170
           RSWDFLGLPIPSY+ +R+             DVVV IFDSG+WPES+SFE+   +  +PC
Sbjct: 65  RSWDFLGLPIPSYTNNRSARFSLHLPSYGDHDVVVAIFDSGVWPESKSFEESEGIGRIPC 124

Query: 171 SWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAEYGGLNTSG-NPEFESPRDFLGHGTH 230
           +WKGKCVK YRFNP+ ACNRKLIGARYYLKGFEA+YG LNT+  NPEF SPRDFLGHGTH
Sbjct: 125 NWKGKCVKGYRFNPASACNRKLIGARYYLKGFEAQYGALNTTADNPEFRSPRDFLGHGTH 184

Query: 231 TASTAVGAIVEDVSF-TGSSLGKGIARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFD 290
           TASTAVGA+V +V F T SSL KG ARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFD
Sbjct: 185 TASTAVGAVVHNVGFPTSSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFD 244

Query: 291 DALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVS 350
           DAL DGVDVISASFG  PPL P FES+SAIGSFH MQ+GVSVVFSAGNDG + SLVQNVS
Sbjct: 245 DALHDGVDVISASFGERPPLIPLFESASAIGSFHAMQRGVSVVFSAGNDGSHPSLVQNVS 304

Query: 351 PWSICVAASTMDRTFPTQILIQNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIR 410
           PWSICVAASTMDRTFPT I I N  SIMGESLIT NIIN KLA+AINYF DG+CER +IR
Sbjct: 305 PWSICVAASTMDRTFPTTIFILNHFSIMGESLITRNIINVKLADAINYFNDGICERENIR 364

Query: 411 KDRKSGAGKVVVCFSTAGPVSMGDAQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDI 470
           K  KSG GKVVVCFST G VS+  AQ+A+ AINASALIFGAPPT +LPDLDL+PTVRIDI
Sbjct: 365 KGGKSGKGKVVVCFSTIGQVSIATAQEAVKAINASALIFGAPPTTELPDLDLIPTVRIDI 424

Query: 471 THATQIRNFLAELPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPXXXXXXXXXXXXXXXX 530
             ATQIRNFLAELPRLP+VEI  ARSVIGKSVAP+VAYFSSRGP                
Sbjct: 425 HQATQIRNFLAELPRLPMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPG 484

Query: 531 XNILAAWPPETAPTVRPTSTEGESQRQ----VKWNFQSGTSMSCPHISGVVALIKSLHPS 590
            NILAAWPPETAPTVRP+    E        VKWNFQSGTSMSCPH+SGVVALIKS+HP+
Sbjct: 485 VNILAAWPPETAPTVRPSGKINEXXXXXXXGVKWNFQSGTSMSCPHVSGVVALIKSVHPN 544

Query: 591 WSPAAIRSALVTTATKKDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTAN 650
           WSPAAIRSA++TTATK DS+ NTILAGGS K SDPFDIG GQVNP+ A+NPGL+YD+T N
Sbjct: 545 WSPAAIRSAIITTATKIDSSGNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTN 604

Query: 651 DYIIFLCNIGYTEQQI-RMIVNPSPQTLVCCPPFLSTSVANLNYPSITLANLKSTTTIKR 710
           DYI FLCNIGYT+QQI  +I+NPSP    CC    +T +AN NYPSITLANL+STTTI+R
Sbjct: 605 DYITFLCNIGYTDQQISNLILNPSPH--FCCRQSTAT-IANFNYPSITLANLRSTTTIRR 664

Query: 711 TVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFG 770
            VRNV+ NKNAIYFL++ PP GV+V VWPR+L FS +RQ +SYYITITPL+K+RGRY FG
Sbjct: 665 IVRNVSLNKNAIYFLRVLPPYGVRVQVWPRVLFFSCYRQQISYYITITPLRKSRGRYGFG 724

Query: 771 EIEWSDGFHRVTSPLVVRVSS 782
           EI+W + FH VTSPLVVR+++
Sbjct: 725 EIQWFNRFHTVTSPLVVRLAT 742

BLAST of Carg20247 vs. TrEMBL
Match: tr|A0A2C9W5W8|A0A2C9W5W8_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_03G086500 PE=4 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 1.4e-261
Identity = 466/762 (61.15%), Postives = 583/762 (76.51%), Query Frame = 0

Query: 23  ITRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSAKLNAS 82
           I+ VYIVYLG  R  D  +TS SH++LLS VFASEE+ K+++LYSYK SFSGFSA LN++
Sbjct: 25  ISHVYIVYLGLNRFHDPIVTSNSHIQLLSNVFASEEEAKQSLLYSYKHSFSGFSAMLNST 84

Query: 83  QAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADGADVVV 142
           QA TL+K++ VIS+F+SK++ELHTTRSWDFLGL +     S T  TP   +L+ G DVVV
Sbjct: 85  QAATLAKIKEVISVFRSKSLELHTTRSWDFLGLTM----DSTTAATP--LQLSYGDDVVV 144

Query: 143 GIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLKGFEAE 202
           GIFD+GIWPES+SF+++  + P+P +WKG+CV+   F P  ACNRKLIGARYYLKGFE +
Sbjct: 145 GIFDTGIWPESDSFKEEPHLRPIPPTWKGQCVEGEGFEPRKACNRKLIGARYYLKGFEQQ 204

Query: 203 YGGLNT-SGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPWARLA 262
           +G LNT  GN E+ S RDFLGHGTH ASTAVG+ + + SF G   GKG ARGGAP ARLA
Sbjct: 205 FGPLNTRGGNSEYRSARDFLGHGTHIASTAVGSTIRNASFFG--FGKGNARGGAPRARLA 264

Query: 263 VYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGSFHGM 322
           VYK+CW K+++GKC ++D++AAFDDAL DGV++ISASFG  PPLAPFF SSS IG+FH M
Sbjct: 265 VYKICWSKNFDGKCAESDILAAFDDALRDGVNIISASFGAPPPLAPFFASSSDIGAFHAM 324

Query: 323 QKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESLITTN 382
           Q GV+VVFSAGNDGP  SLV NVSPWSICVAAST DRTFP QI++ + +SIMGES IT  
Sbjct: 325 QFGVNVVFSAGNDGPNPSLVGNVSPWSICVAASTTDRTFPAQIVLDSNLSIMGESFITRE 384

Query: 383 IINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSM-GDAQQALNAINAS 442
            I  KLANA+ YF +G+C   +  K  +   GKV++CFS  GPV + G AQ A+   N S
Sbjct: 385 -IKGKLANAVMYFTNGICMMENWTK--RLATGKVILCFSNIGPVPLSGIAQVAVKTANGS 444

Query: 443 ALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSVAPS 502
            LIF  PPT Q+ D+D++PTVR+DI+  TQI N+LA+LP+L VV I  +R+VIGKS AP 
Sbjct: 445 GLIFVEPPTNQIADIDIIPTVRVDISQGTQILNYLAQLPKLSVVRILPSRTVIGKSPAPV 504

Query: 503 VAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNFQSG 562
           VA FSSRGP                 NILAAWPP+T PT+ P+       R V+WNFQSG
Sbjct: 505 VAPFSSRGPSSISPDFLKPDLTAPGINILAAWPPKTPPTLLPS-----DDRFVEWNFQSG 564

Query: 563 TSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGSTKPSDPFDIG 622
           TSMS PH+SGV ALIKS HP WSPAAIRSAL+TTAT KD+  ++IL GGS + SDPFD+G
Sbjct: 565 TSMSSPHVSGVAALIKSAHPHWSPAAIRSALMTTATTKDTALDSILVGGSMEVSDPFDMG 624

Query: 623 GGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQT-LVCCPPFLSTSV 682
            G +NPLKA++PGLVYDM   DYI+FLCNIGYT++QI+M++ PSP T  + C     T++
Sbjct: 625 SGHINPLKAMDPGLVYDMKTRDYIVFLCNIGYTQEQIKMMILPSPGTDSISCSNIPKTNM 684

Query: 683 ANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFSWFRQ 742
            NLNYPSIT+++L+S+TTIKRTVRNV + KNAIYF+++  PNGV+V++WPR+L+FS F++
Sbjct: 685 -NLNYPSITVSDLQSSTTIKRTVRNVGSKKNAIYFVRVAKPNGVEVVIWPRVLIFSCFKE 744

Query: 743 HVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 782
            VSYY+T+ PLKK++GRYDFGEI WSDG H V SPLVV V++
Sbjct: 745 EVSYYVTLKPLKKSQGRYDFGEIVWSDGLHDVRSPLVVLVNN 769

BLAST of Carg20247 vs. TrEMBL
Match: tr|A0A2H5PYV9|A0A2H5PYV9_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_180440 PE=4 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.5e-260
Identity = 471/766 (61.49%), Postives = 589/766 (76.89%), Query Frame = 0

Query: 18  NETFFITRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSA 77
           + T   + VYIVYLGH R  D  L S+SHL+LLS VFASEED KR++LYSYK SFSGFSA
Sbjct: 21  HSTSTASHVYIVYLGHNRHCDPNLISKSHLQLLSSVFASEEDAKRSLLYSYKYSFSGFSA 80

Query: 78  KLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADG 137
           KLN+SQA +L++ME VISIF+S+ ++LHTTRSWDF+GL I   +G  T       +LA G
Sbjct: 81  KLNSSQAASLAEMEEVISIFESQVLKLHTTRSWDFMGL-ILDNTGEVT-----PVQLAYG 140

Query: 138 ADVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLK 197
            D+VVGIFD+G+WPESESF+++  MSP+P SWKG CV+  +F+P  ACNRKLIGARYY+K
Sbjct: 141 DDIVVGIFDTGVWPESESFQEEPSMSPIPSSWKGTCVRGEKFDPQKACNRKLIGARYYVK 200

Query: 198 GFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPW 257
           GFE EYG LN S N E+ S RDFLGHGTHTASTA G+I ++  F G  LG+GIARGGAP 
Sbjct: 201 GFEEEYGPLNASTNREYRSARDFLGHGTHTASTAAGSIAKNAGFFG--LGRGIARGGAPR 260

Query: 258 ARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGS 317
           ARLAVYK+CWGKD +GKCT+AD++AAFDDAL DGVDVISASFG SPPL PFF S++ IGS
Sbjct: 261 ARLAVYKICWGKDSDGKCTEADILAAFDDALHDGVDVISASFGESPPLRPFFASNADIGS 320

Query: 318 FHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESL 377
           F+ MQ GV+VVFS GNDGP  SLVQNV+PWSICVAAS++DRTFPT+I++ +  SI+GES 
Sbjct: 321 FNAMQHGVTVVFSGGNDGPEPSLVQNVAPWSICVAASSIDRTFPTEIVVNSDFSIVGESF 380

Query: 378 ITTNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAI 437
           I+T  + AKL  A  YF DG+C+  +     +   G+VV+CFST G V   +A+ A    
Sbjct: 381 ISTE-VKAKLVEAFTYFADGICKCENWM--GRKATGRVVLCFSTMGSVKTEEAEAAAKKA 440

Query: 438 NASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSV 497
           NAS LIF  P T  + ++D++PTVRIDI   TQ+R++LA+ PRLP+V+++ +++ IGK  
Sbjct: 441 NASGLIFAEPMTELIAEVDIIPTVRIDIAQGTQLRDYLAQFPRLPIVQLKPSKTSIGKVP 500

Query: 498 APSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNF 557
           AP+VAYFSSRGP   XXXXXXXXXXXXX  +LAAWPP T PT+ P+       R VKWNF
Sbjct: 501 APTVAYFSSRGPSSIXXXXXXXXXXXXXIGVLAAWPPNTPPTLLPS-----DGRSVKWNF 560

Query: 558 QSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGSTKPSDPF 617
           QSGTSMSCPH+SGVVALIKS HP+WSPAAIRSAL+TTA  +D++ ++ILAGGS K SDPF
Sbjct: 561 QSGTSMSCPHVSGVVALIKSAHPNWSPAAIRSALMTTAYTRDTSHDSILAGGSMKVSDPF 620

Query: 618 DIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTL--VCCPPFL 677
           DIG G +NP+KA++PGL+YD+   DYI+FL NIGYT+ QI  I  PSP       CP   
Sbjct: 621 DIGAGHINPMKAMDPGLIYDLKPTDYIVFLRNIGYTQDQINKIFLPSPDETERTSCPQAH 680

Query: 678 STSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFS 737
               + +NYPSIT++NL+ST TIKRTV+NV   KNAIYF  +  P GV+V+VWPR+L+FS
Sbjct: 681 KIPNSFINYPSITVSNLQSTMTIKRTVKNVGQKKNAIYFASVVKPGGVEVVVWPRVLVFS 740

Query: 738 WFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 782
           WF++ VSYY+++ PLK ++GR+DFG+I WSDGFH V SPLVV V++
Sbjct: 741 WFKEEVSYYVSLKPLKMSQGRFDFGQIVWSDGFHYVRSPLVVFVNN 770

BLAST of Carg20247 vs. TrEMBL
Match: tr|A0A067EU78|A0A067EU78_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g003970mg PE=4 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.0e-259
Identity = 470/766 (61.36%), Postives = 588/766 (76.76%), Query Frame = 0

Query: 18  NETFFITRVYIVYLGHTRLEDATLTSESHLRLLSKVFASEEDGKRAMLYSYKRSFSGFSA 77
           + T   + VYIVYLGH R  D  L S+SHL+LLS VFASEED KR++LY YK SFSGFSA
Sbjct: 21  HSTSTASHVYIVYLGHNRHCDPNLISKSHLQLLSSVFASEEDAKRSLLYGYKYSFSGFSA 80

Query: 78  KLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGSRTFDTPRSRKLADG 137
           KLN+SQA +L++ME VISIF+S+ ++LHTTRSWDF+GL I   +G  T       +LA G
Sbjct: 81  KLNSSQAASLAEMEEVISIFESQVLKLHTTRSWDFMGL-ILDNTGEVT-----PVQLAYG 140

Query: 138 ADVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSLACNRKLIGARYYLK 197
            D+VVGIFD+G+WPESESF+++  MSP+P SWKG CV+  +F+P  ACNRKLIGARYY+K
Sbjct: 141 DDIVVGIFDTGVWPESESFQEEPSMSPIPSSWKGTCVRGEKFDPQKACNRKLIGARYYVK 200

Query: 198 GFEAEYGGLNTSGNPEFESPRDFLGHGTHTASTAVGAIVEDVSFTGSSLGKGIARGGAPW 257
           GFE EYG LN S N E+ S RDFLGHGTHTASTA G+I ++  F G  LG+GIARGGAP 
Sbjct: 201 GFEEEYGPLNASTNREYRSARDFLGHGTHTASTAAGSIAKNAGFFG--LGRGIARGGAPR 260

Query: 258 ARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFGGSPPLAPFFESSSAIGS 317
           ARLAVYK+CWGKD +GKCT+AD++AAFDDAL DGVDVISASFG SPPL PFF S++ IGS
Sbjct: 261 ARLAVYKICWGKDSDGKCTEADILAAFDDALHDGVDVISASFGESPPLRPFFASNADIGS 320

Query: 318 FHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTFPTQILIQNRISIMGESL 377
           F+ MQ GV+VVFS GNDGP  SLVQNV+PWSICVAAS++DRTFPT+I++ +  SI+GES 
Sbjct: 321 FNAMQHGVTVVFSGGNDGPEPSLVQNVAPWSICVAASSIDRTFPTEIVVNSDFSIVGESF 380

Query: 378 ITTNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVVCFSTAGPVSMGDAQQALNAI 437
           I+T  + AKL  A  YF DG+C+  +     +   G+VV+CFST G V   +A+ A    
Sbjct: 381 ISTE-VKAKLVEAFTYFADGICKCENWM--GRKATGRVVLCFSTMGSVKTEEAEAAAKKA 440

Query: 438 NASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAELPRLPVVEIRAARSVIGKSV 497
           NAS LIF  P T  + ++D++PTVRIDI   TQ+R++LA+ PRLP+V+++ +++ IGK  
Sbjct: 441 NASGLIFAEPMTELIAEVDIIPTVRIDIAQGTQLRDYLAQFPRLPIVQLKPSKTSIGKVP 500

Query: 498 APSVAYFSSRGPXXXXXXXXXXXXXXXXXNILAAWPPETAPTVRPTSTEGESQRQVKWNF 557
           AP+VAYFSSRGP   XXXXXXXXXXXXX  +LAAWPP T PT+ P+       R VKWNF
Sbjct: 501 APTVAYFSSRGPSSIXXXXXXXXXXXXXIGVLAAWPPNTPPTLLPS-----DGRSVKWNF 560

Query: 558 QSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATKKDSTRNTILAGGSTKPSDPF 617
           QSGTSMSCPH+SGVVALIKS HP+WSPAAIRSAL+TTA  +D++ ++ILAGGS K SDPF
Sbjct: 561 QSGTSMSCPHVSGVVALIKSAHPNWSPAAIRSALMTTAYTRDTSHDSILAGGSMKVSDPF 620

Query: 618 DIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQIRMIVNPSPQTL--VCCPPFL 677
           DIG G +NP+KA++PGL+YD+   DYI+FL NIGYT+ QI  I  PSP       CP   
Sbjct: 621 DIGAGHINPMKAMDPGLIYDLKPTDYIVFLRNIGYTQDQINKIFLPSPDETERTSCPQAH 680

Query: 678 STSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYFLKLNPPNGVQVLVWPRILLFS 737
               + +NYPSIT++NL+ST TIKRTV+NV   KNAIYF  +  P GV+V+VWPR+L+FS
Sbjct: 681 KIPNSFINYPSITVSNLQSTMTIKRTVKNVGQKKNAIYFASVVKPGGVEVVVWPRVLVFS 740

Query: 738 WFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSPLVVRVSS 782
           WF++ VSYY+++ PLK ++GR+DFG+I WSDGFH V SPLVV V++
Sbjct: 741 WFKEEVSYYVSLKPLKMSQGRFDFGQIVWSDGFHYVRSPLVVFVNN 770