Lsi10G009240.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G009240.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ethylene-responsive transcription factor
Location	chr10 : 13706294 .. 13706863 (-)
Sequence length	570

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGTTCAGTTGAGATAAGACAGCATAAAGAGGTTAAACTTAAATCAGTTGGGAAATGCAGTAACAACAGATCTGGTAAACGCAGCTTTGTTGGTGTTAGGCAAAGGCCTTCTGGGAAATGGGTTGCAGAGATCAAAGATACAACTCATGATATCAGAATGTGGTTGGGAACCTTCAACTCAGCGGAGGAGGCTGCAACAGCTTACGATGAAGCCGCCCGCCTCCTCCGCGGTCCTAATGCTCGAACCAACTTCAATCATACTTCCAAATCCACCTCCACCCTCTCTTTCAAAATCAGAAACCTTCTCACCAACAAAATCGCTTTGAAACGAACCTCAATCATTGAAACTCATGCTCCAACCAGCCAAGAGATGCACATGTTTCATAATAGTTCATTCTGCTGTGGAGAATTTGAGATGGGGTTTTGTCACTTCACCCATTCTTGCTGCCATCTTCCTCTTGAGTTCAACTTGGATATGGATGAGCCTGTGTGTGCATTCAATGGAATGACTGAACATTTAGATAATACATATAATGATGCTTTTGATACTGAGCCTCACATATGA

mRNA sequence

ATGGAGGGTTCAGTTGAGATAAGACAGCATAAAGAGGTTAAACTTAAATCAGTTGGGAAATGCAGTAACAACAGATCTGGTAAACGCAGCTTTGTTGGTGTTAGGCAAAGGCCTTCTGGGAAATGGGTTGCAGAGATCAAAGATACAACTCATGATATCAGAATGTGGTTGGGAACCTTCAACTCAGCGGAGGAGGCTGCAACAGCTTACGATGAAGCCGCCCGCCTCCTCCGCGGTCCTAATGCTCGAACCAACTTCAATCATACTTCCAAATCCACCTCCACCCTCTCTTTCAAAATCAGAAACCTTCTCACCAACAAAATCGCTTTGAAACGAACCTCAATCATTGAAACTCATGCTCCAACCAGCCAAGAGATGCACATGTTTCATAATAGTTCATTCTGCTGTGGAGAATTTGAGATGGGGTTTTGTCACTTCACCCATTCTTGCTGCCATCTTCCTCTTGAGTTCAACTTGGATATGGATGAGCCTGTGTGTGCATTCAATGGAATGACTGAACATTTAGATAATACATATAATGATGCTTTTGATACTGAGCCTCACATATGA

Coding sequence (CDS)

ATGGAGGGTTCAGTTGAGATAAGACAGCATAAAGAGGTTAAACTTAAATCAGTTGGGAAATGCAGTAACAACAGATCTGGTAAACGCAGCTTTGTTGGTGTTAGGCAAAGGCCTTCTGGGAAATGGGTTGCAGAGATCAAAGATACAACTCATGATATCAGAATGTGGTTGGGAACCTTCAACTCAGCGGAGGAGGCTGCAACAGCTTACGATGAAGCCGCCCGCCTCCTCCGCGGTCCTAATGCTCGAACCAACTTCAATCATACTTCCAAATCCACCTCCACCCTCTCTTTCAAAATCAGAAACCTTCTCACCAACAAAATCGCTTTGAAACGAACCTCAATCATTGAAACTCATGCTCCAACCAGCCAAGAGATGCACATGTTTCATAATAGTTCATTCTGCTGTGGAGAATTTGAGATGGGGTTTTGTCACTTCACCCATTCTTGCTGCCATCTTCCTCTTGAGTTCAACTTGGATATGGATGAGCCTGTGTGTGCATTCAATGGAATGACTGAACATTTAGATAATACATATAATGATGCTTTTGATACTGAGCCTCACATATGA

Protein sequence

MEGSVEIRQHKEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHAPTSQEMHMFHNSSFCCGEFEMGFCHFTHSCCHLPLEFNLDMDEPVCAFNGMTEHLDNTYNDAFDTEPHI

Homology

[Close]

BLAST of Lsi10G009240.1 vs. Swiss-Prot
Match: RA211_ARATH (Ethylene-responsive transcription factor RAP2-11 OS=Arabidopsis thaliana GN=RAP2-11 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-25
Identity = 63/100 (63.00%), Postives = 72/100 (72.00%), Query Frame = 1


Query: 24  NRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPNAR 83
           ++  K  FVGVRQRPSGKWVAEIKDTT  IRMWLGTF +AEEAA AYDEAA LLRG N R
Sbjct: 15  SKGNKTKFVGVRQRPSGKWVAEIKDTTQKIRMWLGTFETAEEAARAYDEAACLLRGSNTR 74

Query: 84  TNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHAPTS 124
           TNF +   + S LS KIRNLL  K ++K+    + H P S
Sbjct: 75  TNFANHFPNNSQLSLKIRNLLHQKQSMKQQQ-QQQHKPVS 113

[Close]

BLAST of Lsi10G009240.1 vs. Swiss-Prot
Match: CRF2_ARATH (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana GN=CRF2 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.2e-16
Identity = 45/86 (52.33%), Postives = 58/86 (67.44%), Query Frame = 1


Query: 8   RQHKEVKLKS----VGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSA 67
           RQ + VK +S    V   +   +G++ F GVRQRP GKW AEI+D    +R+WLGT+N+A
Sbjct: 95  RQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWGKWAAEIRDPLKRVRLWLGTYNTA 154

Query: 68  EEAATAYDEAARLLRGPNARTNFNHT 90
           EEAA  YD AA  LRGP+A TNF+ T
Sbjct: 155 EEAAMVYDNAAIQLRGPDALTNFSVT 180

[Close]

BLAST of Lsi10G009240.1 vs. Swiss-Prot
Match: ERF1_ORYSJ (Ethylene-responsive transcription factor 1 OS=Oryza sativa subsp. japonica GN=EREBP1 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.4e-15
Identity = 39/65 (60.00%), Postives = 45/65 (69.23%), Query Frame = 1


Query: 22  SNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPN 81
           S  R  K  F G+RQRP GKW AEI+D    +R+WLGTFNSAEEAA AYD  AR +RG  
Sbjct: 111 SAKRKRKNQFRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNSAEEAARAYDAEARRIRGKK 170

Query: 82  ARTNF 87
           A+ NF
Sbjct: 171 AKVNF 175

[Close]

BLAST of Lsi10G009240.1 vs. Swiss-Prot
Match: CRF1_ARATH (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana GN=CRF1 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.5e-15
Identity = 38/58 (65.52%), Postives = 44/58 (75.86%), Query Frame = 1


Query: 29  RSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPNARTNF 87
           R F GVRQRP GKW AEI+D +  +R+WLGTF++AEEAA  YD AA  LRGPNA  NF
Sbjct: 85  RKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNAAIQLRGPNAELNF 142

[Close]

BLAST of Lsi10G009240.1 vs. Swiss-Prot
Match: RAP22_ARATH (Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana GN=RAP2-2 PE=1 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.2e-14
Identity = 50/133 (37.59%), Postives = 70/133 (52.63%), Query Frame = 1


Query: 11  KEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAY 70
           K V+     + S+ R  K  + G+RQRP GKW AEI+D     R WLGTF++AEEAA AY
Sbjct: 108 KTVESAEQAEKSSKRKRKNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAY 167

Query: 71  DEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHAPTSQEMHMFH 130
           D AAR +RG  A+ NF    K+ S +S K  +  TN +        ++     Q  H+  
Sbjct: 168 DAAARRIRGTKAKVNFPE-EKNPSVVSQKRPSAKTNNLQKSVAKPNKSVTLVQQPTHL-- 227

Query: 131 NSSFCCGEFEMGF 144
           +  +C   F+  F
Sbjct: 228 SQQYCNNSFDNSF 237

[Close]

BLAST of Lsi10G009240.1 vs. TrEMBL
Match: A0A0A0L1N6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268100 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.2e-67
Identity = 136/193 (70.47%), Postives = 151/193 (78.24%), Query Frame = 1


Query: 1   MEGSVEIRQHKEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTF 60
           ME SVE ++H++V  K VGKC   RSGKRSFVGVRQRPSGKWVAEIKD THDIRMWLGTF
Sbjct: 1   MESSVEKQEHRDVAFKLVGKCIKKRSGKRSFVGVRQRPSGKWVAEIKDATHDIRMWLGTF 60

Query: 61  NSAEEAATAYDEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHA 120
           N+ EEAA AYDEAA LLRG NARTNF   S S+S LSFKIRNLL +KI LKR+SI ETHA
Sbjct: 61  NTPEEAARAYDEAACLLRGSNARTNFTLASNSSSALSFKIRNLLIHKITLKRSSITETHA 120

Query: 121 -----PTSQEMHMFHNS---SFCCGEFEMGFCHFTHSCCHLPLEFNLDMDEPVCAFNGMT 180
                 T+QEMHMF N+   S C GE E+GFCHFTHSCC LPL FN DMDE + A NG+T
Sbjct: 121 HVAHSETNQEMHMFDNNAVWSSCNGEIEVGFCHFTHSCCDLPLGFNFDMDESLSALNGIT 180

Query: 181 EHLDNTYNDAFDT 186
           +HL N Y+DAFDT
Sbjct: 181 QHLGNAYDDAFDT 193

[Close]

BLAST of Lsi10G009240.1 vs. TrEMBL
Match: K4ASC9_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.3e-25
Identity = 67/105 (63.81%), Postives = 77/105 (73.33%), Query Frame = 1


Query: 28  KRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPNARTNFN 87
           +R FVGVRQRPSGKWVAEIK+TT  IRMWLGTF+SAEEAA AYDEAA LLRG NARTNF+
Sbjct: 16  QRKFVGVRQRPSGKWVAEIKNTTQKIRMWLGTFDSAEEAARAYDEAACLLRGSNARTNFH 75

Query: 88  HTSKSTSTLSFKIRNLLT-----NKIALKRTSIIETHAPTSQEMH 128
           + +  +  LS KIRNL+      NKI  K TS   +  PTS  +H
Sbjct: 76  NHTPVSPALSMKIRNLINHKKSLNKINHKSTSTSSSSIPTSSTIH 120

[Close]

BLAST of Lsi10G009240.1 vs. TrEMBL
Match: B9GY71_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s21090g PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.6e-25
Identity = 66/95 (69.47%), Postives = 72/95 (75.79%), Query Frame = 1


Query: 20  KCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRG 79
           +C +N S K  FVGVRQRPSGKWVAEIKDTT  IRMWLGTF +AEEAA AYDEAA LLRG
Sbjct: 24  RCLSNNS-KNKFVGVRQRPSGKWVAEIKDTTQKIRMWLGTFETAEEAARAYDEAACLLRG 83

Query: 80  PNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTS 115
            N RTNFN    S S +S KIRNLL  K +LK+ S
Sbjct: 84  SNTRTNFNTHVSSNSPISTKIRNLLNRKRSLKQNS 117

[Close]

BLAST of Lsi10G009240.1 vs. TrEMBL
Match: A0A067EKK6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g023169mg PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.7e-24
Identity = 69/121 (57.02%), Postives = 78/121 (64.46%), Query Frame = 1


Query: 16  KSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAAR 75
           K   K S N   K  FVGVRQRPSGKWVAEIKDTT  IRMWLGTF +AEEAA AYDEAA 
Sbjct: 17  KIKAKSSTNPGNKSKFVGVRQRPSGKWVAEIKDTTKKIRMWLGTFETAEEAARAYDEAAC 76

Query: 76  LLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHAPTSQEMHMFHNSSFC 135
           LLRG N RTNF     + S +S KIRNLL +K +LK++S   T +  S        S+  
Sbjct: 77  LLRGSNTRTNFATPVFTNSHISLKIRNLLNHKRSLKQSSPSTTTSTGSNTTAPIKPSTIV 136

Query: 136 C 137
           C
Sbjct: 137 C 137

[Close]

BLAST of Lsi10G009240.1 vs. TrEMBL
Match: A0A078C1K8_BRANA (BnaC03g09750D protein OS=Brassica napus GN=BnaC03g09750D PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.7e-24
Identity = 70/126 (55.56%), Postives = 83/126 (65.87%), Query Frame = 1


Query: 9   QHKEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAAT 68
           Q +++K KS G      + K  FVGVRQRPSGKWVAEIKDTT  IRMWLGTF +AEEAA 
Sbjct: 8   QKQKIKRKSKG------TNKTKFVGVRQRPSGKWVAEIKDTTQKIRMWLGTFETAEEAAR 67

Query: 69  AYDEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHAPTSQEMHM 128
           AYDEAA LLRG N RTNF +   + S LS KIRNLL  K +LK     + + P S  +  
Sbjct: 68  AYDEAACLLRGSNTRTNFANHFPNNSQLSLKIRNLLHQKQSLKHQQQQQQNKPASSFVE- 125

Query: 129 FHNSSF 135
            HN ++
Sbjct: 128 -HNINY 125

[Close]

BLAST of Lsi10G009240.1 vs. TAIR10
Match: AT5G19790.1 (AT5G19790.1 related to AP2 11)

HSP 1 Score: 116.7 bits (291), Expect = 1.6e-26
Identity = 63/100 (63.00%), Postives = 72/100 (72.00%), Query Frame = 1


Query: 24  NRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPNAR 83
           ++  K  FVGVRQRPSGKWVAEIKDTT  IRMWLGTF +AEEAA AYDEAA LLRG N R
Sbjct: 15  SKGNKTKFVGVRQRPSGKWVAEIKDTTQKIRMWLGTFETAEEAARAYDEAACLLRGSNTR 74

Query: 84  TNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHAPTS 124
           TNF +   + S LS KIRNLL  K ++K+    + H P S
Sbjct: 75  TNFANHFPNNSQLSLKIRNLLHQKQSMKQQQ-QQQHKPVS 113

[Close]

BLAST of Lsi10G009240.1 vs. TAIR10
Match: AT4G23750.1 (AT4G23750.1 cytokinin response factor 2)

HSP 1 Score: 85.9 bits (211), Expect = 2.9e-17
Identity = 45/86 (52.33%), Postives = 58/86 (67.44%), Query Frame = 1


Query: 8   RQHKEVKLKS----VGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSA 67
           RQ + VK +S    V   +   +G++ F GVRQRP GKW AEI+D    +R+WLGT+N+A
Sbjct: 95  RQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWGKWAAEIRDPLKRVRLWLGTYNTA 154

Query: 68  EEAATAYDEAARLLRGPNARTNFNHT 90
           EEAA  YD AA  LRGP+A TNF+ T
Sbjct: 155 EEAAMVYDNAAIQLRGPDALTNFSVT 180

[Close]

BLAST of Lsi10G009240.1 vs. TAIR10
Match: AT4G11140.1 (AT4G11140.1 cytokinin response factor 1)

HSP 1 Score: 82.0 bits (201), Expect = 4.2e-16
Identity = 38/58 (65.52%), Postives = 44/58 (75.86%), Query Frame = 1


Query: 29  RSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPNARTNF 87
           R F GVRQRP GKW AEI+D +  +R+WLGTF++AEEAA  YD AA  LRGPNA  NF
Sbjct: 85  RKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNAAIQLRGPNAELNF 142

[Close]

BLAST of Lsi10G009240.1 vs. TAIR10
Match: AT3G14230.1 (AT3G14230.1 related to AP2 2)

HSP 1 Score: 80.5 bits (197), Expect = 1.2e-15
Identity = 50/133 (37.59%), Postives = 70/133 (52.63%), Query Frame = 1


Query: 11  KEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAY 70
           K V+     + S+ R  K  + G+RQRP GKW AEI+D     R WLGTF++AEEAA AY
Sbjct: 108 KTVESAEQAEKSSKRKRKNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAY 167

Query: 71  DEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHAPTSQEMHMFH 130
           D AAR +RG  A+ NF    K+ S +S K  +  TN +        ++     Q  H+  
Sbjct: 168 DAAARRIRGTKAKVNFPE-EKNPSVVSQKRPSAKTNNLQKSVAKPNKSVTLVQQPTHL-- 227

Query: 131 NSSFCCGEFEMGF 144
           +  +C   F+  F
Sbjct: 228 SQQYCNNSFDNSF 237

[Close]

BLAST of Lsi10G009240.1 vs. TAIR10
Match: AT1G53910.1 (AT1G53910.1 related to AP2 12)

HSP 1 Score: 79.7 bits (195), Expect = 2.1e-15
Identity = 40/88 (45.45%), Postives = 53/88 (60.23%), Query Frame = 1


Query: 22  SNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRGPN 81
           S NR  K  + G+RQRP GKW AEI+D     R+WLGTF +AEEAA AYD AAR +RG  
Sbjct: 116 SANRKRKNQYRGIRQRPWGKWAAEIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSK 175

Query: 82  ARTNFNHTSKSTSTLSFKIRNLLTNKIA 110
           A+ NF   +   ++    ++  L   +A
Sbjct: 176 AKVNFPEENMKANSQKRSVKANLQKPVA 203

[Close]

BLAST of Lsi10G009240.1 vs. NCBI nr
Match: gi|659098050|ref|XP_008449953.1| (PREDICTED: ethylene-responsive transcription factor RAP2-11 [Cucumis melo])

HSP 1 Score: 268.9 bits (686), Expect = 6.9e-69
Identity = 139/193 (72.02%), Postives = 153/193 (79.27%), Query Frame = 1


Query: 1   MEGSVEIRQHKEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTF 60
           MEGSVE ++ ++V  K VGKC   RSGKRSFVGVRQRPSGKWVAEIKD THDIRMWLGTF
Sbjct: 1   MEGSVEKQERRDVAFKLVGKCIKKRSGKRSFVGVRQRPSGKWVAEIKDATHDIRMWLGTF 60

Query: 61  NSAEEAATAYDEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHA 120
           N+AEEAA AYDEAA LLRG NARTNF   S S+STLSFKIRNLL +KI LKR+SI ETHA
Sbjct: 61  NTAEEAARAYDEAACLLRGSNARTNFTLASNSSSTLSFKIRNLLIHKITLKRSSITETHA 120

Query: 121 -----PTSQEMHMFHNSSF---CCGEFEMGFCHFTHSCCHLPLEFNLDMDEPVCAFNGMT 180
                 T+QE HMF N +    C GE E+GFCHFTHSCCHLPL FN DMDE + A NG+T
Sbjct: 121 HVAHSETNQETHMFDNDTVWNSCNGEIEVGFCHFTHSCCHLPLGFNFDMDESLSAPNGIT 180

Query: 181 EHLDNTYNDAFDT 186
           EHL NTY+DAFDT
Sbjct: 181 EHLGNTYDDAFDT 193

[Close]

BLAST of Lsi10G009240.1 vs. NCBI nr
Match: gi|449463881|ref|XP_004149659.1| (PREDICTED: ethylene-responsive transcription factor ERF087 [Cucumis sativus])

HSP 1 Score: 264.2 bits (674), Expect = 1.7e-67
Identity = 136/193 (70.47%), Postives = 151/193 (78.24%), Query Frame = 1


Query: 1   MEGSVEIRQHKEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTF 60
           ME SVE ++H++V  K VGKC   RSGKRSFVGVRQRPSGKWVAEIKD THDIRMWLGTF
Sbjct: 1   MESSVEKQEHRDVAFKLVGKCIKKRSGKRSFVGVRQRPSGKWVAEIKDATHDIRMWLGTF 60

Query: 61  NSAEEAATAYDEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTSIIETHA 120
           N+ EEAA AYDEAA LLRG NARTNF   S S+S LSFKIRNLL +KI LKR+SI ETHA
Sbjct: 61  NTPEEAARAYDEAACLLRGSNARTNFTLASNSSSALSFKIRNLLIHKITLKRSSITETHA 120

Query: 121 -----PTSQEMHMFHNS---SFCCGEFEMGFCHFTHSCCHLPLEFNLDMDEPVCAFNGMT 180
                 T+QEMHMF N+   S C GE E+GFCHFTHSCC LPL FN DMDE + A NG+T
Sbjct: 121 HVAHSETNQEMHMFDNNAVWSSCNGEIEVGFCHFTHSCCDLPLGFNFDMDESLSALNGIT 180

Query: 181 EHLDNTYNDAFDT 186
           +HL N Y+DAFDT
Sbjct: 181 QHLGNAYDDAFDT 193

[Close]

BLAST of Lsi10G009240.1 vs. NCBI nr
Match: gi|971562154|ref|XP_015167123.1| (PREDICTED: ethylene-responsive transcription factor RAP2-11 [Solanum tuberosum])

HSP 1 Score: 126.3 bits (316), Expect = 5.6e-26
Identity = 71/123 (57.72%), Postives = 85/123 (69.11%), Query Frame = 1


Query: 17  SVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARL 76
           S+ KCS     +R FVGVRQRPSGKWVAEIK+TT  IRMWLGTF+SAEEAA AYDEAA L
Sbjct: 9   SIKKCSPKVQKQRKFVGVRQRPSGKWVAEIKNTTQKIRMWLGTFDSAEEAARAYDEAACL 68

Query: 77  LRGPNARTNF-NHTSKSTSTLSFKIRNLLTNKIALKR----TSIIETHAPTSQEMHMFHN 135
           LRG NARTNF NH       +S KIRNL+ +K +L +    TS I T   T QE  + ++
Sbjct: 69  LRGSNARTNFHNHAPPINPAVSMKIRNLINHKKSLNKSTSSTSTISTSTTTHQETALSND 128

[Close]

BLAST of Lsi10G009240.1 vs. NCBI nr
Match: gi|224075978|ref|XP_002304856.1| (hypothetical protein POPTR_0003s21090g [Populus trichocarpa])

HSP 1 Score: 122.5 bits (306), Expect = 8.0e-25
Identity = 66/95 (69.47%), Postives = 72/95 (75.79%), Query Frame = 1


Query: 20  KCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTFNSAEEAATAYDEAARLLRG 79
           +C +N S K  FVGVRQRPSGKWVAEIKDTT  IRMWLGTF +AEEAA AYDEAA LLRG
Sbjct: 24  RCLSNNS-KNKFVGVRQRPSGKWVAEIKDTTQKIRMWLGTFETAEEAARAYDEAACLLRG 83

Query: 80  PNARTNFNHTSKSTSTLSFKIRNLLTNKIALKRTS 115
            N RTNFN    S S +S KIRNLL  K +LK+ S
Sbjct: 84  SNTRTNFNTHVSSNSPISTKIRNLLNRKRSLKQNS 117

[Close]

BLAST of Lsi10G009240.1 vs. NCBI nr
Match: gi|645215039|ref|XP_008221743.1| (PREDICTED: ethylene-responsive transcription factor RAP2-11 [Prunus mume])

HSP 1 Score: 122.1 bits (305), Expect = 1.0e-24
Identity = 69/110 (62.73%), Postives = 78/110 (70.91%), Query Frame = 1


Query: 1   MEGSVEIRQHKEVKLKSVGKCSNNRSGKRSFVGVRQRPSGKWVAEIKDTTHDIRMWLGTF 60
           ME  V +  +K+ KLK+  KC         FVGVRQRPSGKWVAEIKDTT  IRMWLGTF
Sbjct: 13  MENGVSVSANKQSKLKT--KCK--------FVGVRQRPSGKWVAEIKDTTKKIRMWLGTF 72

Query: 61  NSAEEAATAYDEAARLLRGPNARTNFNHTSKSTSTLSFKIRNLLTNKIAL 111
            +AEEAA AYDEAA LLRG N RTNF+  + S S LS KIRNLL +K +L
Sbjct: 73  ETAEEAARAYDEAAYLLRGSNTRTNFSTHAPSNSPLSLKIRNLLNHKKSL 112

The following BLAST results are available for this feature:

BLAST of Lsi10G009240.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RA211_ARATH	2.8e-25	63.00	Ethylene-responsive transcription factor RAP2-11 OS=Arabidopsis thaliana GN=RAP2...	[more]
CRF2_ARATH	5.2e-16	52.33	Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana GN=CRF2 PE...	[more]
ERF1_ORYSJ	4.4e-15	60.00	Ethylene-responsive transcription factor 1 OS=Oryza sativa subsp. japonica GN=ER...	[more]
CRF1_ARATH	7.5e-15	65.52	Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana GN=CRF1 PE...	[more]
RAP22_ARATH	2.2e-14	37.59	Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana GN=RAP2-...	[more]

BLAST of Lsi10G009240.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1N6_CUCSA	1.2e-67	70.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268100 PE=4 SV=1	[more]
K4ASC9_SOLLC	4.3e-25	63.81	Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1	[more]
B9GY71_POPTR	5.6e-25	69.47	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s21090g PE=4 SV=1	[more]
A0A067EKK6_CITSI	4.7e-24	57.02	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g023169mg PE=4 SV=1	[more]
A0A078C1K8_BRANA	4.7e-24	55.56	BnaC03g09750D protein OS=Brassica napus GN=BnaC03g09750D PE=4 SV=1	[more]

BLAST of Lsi10G009240.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19790.1	1.6e-26	63.00	related to AP2 11	[more]
AT4G23750.1	2.9e-17	52.33	cytokinin response factor 2	[more]
AT4G11140.1	4.2e-16	65.52	cytokinin response factor 1	[more]
AT3G14230.1	1.2e-15	37.59	related to AP2 2	[more]
AT1G53910.1	2.1e-15	45.45	related to AP2 12	[more]

BLAST of Lsi10G009240.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659098050\|ref\|XP_008449953.1\|	6.9e-69	72.02	PREDICTED: ethylene-responsive transcription factor RAP2-11 [Cucumis melo]	[more]
gi\|449463881\|ref\|XP_004149659.1\|	1.7e-67	70.47	PREDICTED: ethylene-responsive transcription factor ERF087 [Cucumis sativus]	[more]
gi\|971562154\|ref\|XP_015167123.1\|	5.6e-26	57.72	PREDICTED: ethylene-responsive transcription factor RAP2-11 [Solanum tuberosum]	[more]
gi\|224075978\|ref\|XP_002304856.1\|	8.0e-25	69.47	hypothetical protein POPTR_0003s21090g [Populus trichocarpa]	[more]
gi\|645215039\|ref\|XP_008221743.1\|	1.0e-24	62.73	PREDICTED: ethylene-responsive transcription factor RAP2-11 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR017392	AP2/ERF-transcript_factor
IPR016177	DNA-bd_dom_sf
IPR001471	AP2/ERF_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi10G009240	Lsi10G009240	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi10G009240.1.CDS.1	Lsi10G009240.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi10G009240.1	Lsi10G009240.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 31..42 score: 4.6E-7coord: 53..69 score: 4.
IPR001471	AP2/ERF domain	GENE3D	G3DSA:3.30.730.10		coord: 30..88 score: 1.7
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 31..79 score: 1.3
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 30..93 score: 9.0
IPR001471	AP2/ERF domain	PROFILE	PS51032	AP2_ERF	coord: 30..87 score: 19
IPR016177	DNA-binding domain	unknown	SSF54171	DNA-binding domain	coord: 31..88 score: 1.37
IPR017392	AP2/ERF transcription factor ERF/PTI6	PANTHER	PTHR31194	SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR	coord: 28..112 score: 2.4
None	No IPR available	PANTHER	PTHR31194:SF5	SUBFAMILY NOT NAMED	coord: 28..112 score: 2.4