Lsi05G018600.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G018600.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Chaperone protein dnaJ 20
Location	chr05 : 25829776 .. 25831793 (+)
Sequence length	1273

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTAATTCTAAACCGTACAAGTGACTCAGCCTTGGACGTCCCGTATAAATGTAGAAGATCCAGAGAACGGAAGATAGACGGAGAAAAACAGAGAAATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGTATTAGTTCAGTCTACCAATTTTAACTTTGTGTTTTTTTCGTTATTTTAGGGGTAATAATTATGTTTGATTGTTTTTTCGTTTTTTTAGGGGTAATTATGTTTGATTGTTAACAGATTTTGAAATCTCTCGAGTTTCTTTTGCGTTTGCCCTTATTTTTGAAGTGATTTCGTGTTTAATTTCTCAGATGTTGTACAGAGAGATTAGAATGAGTGAAGGATTCGATCTTAATGTTTCTTTGATTTTGGATTTTTGGAGTGACGAAACGAAACGTCGTTTCATTCTGGGTCCTGATTCTCTTTTTCTTTCCTTTTTTGTTCGTCTGACTCTATCCTATACGGATGCAGTAGGATTGGTGGAGACGGATCCTGTTTCTGTTTAACAAGGACCTACAATTGGTTAATCAGTGATTTGTTCTGTAATTAAGAAACGGAATGGGATTGGTCAATCTGCTGTTTTCTGGATATCTTACCCCAAATATGGAATTCCGTTACCATAAACTTTAATTTTCATTACTCTTCTGTTTATCTATATGCAACTTTTTCACATAATATTTCTTTCTGTCTTCATTTAATTGTTGTATTCTAGCAAATCTGTATGAAATCCTTTTTCACCCACTGATTCAAACAAAACATTTGAGTTAATCCCACCTGGGAGTCCTGTTATCTCATTAGAACACATGCCAATTTTTTACTTCCCATTTGAATATCATACACTAAATCTCCAAATTTGATTTTGAATTCAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATTGAATTATATAGATTAAGAAAACCATGTTTGACTTTCAGCATCAGTAAATATAATACGGCCTATTTAGTAACAATAATTTTGTTGTTAGTTTTCTCAATTTCCCCTTTGTAATCTGTGCTATACTATATATAAAAAGGGTTATGAGTGTAAAATCTTTGTTTCCCATCTTAGACCTCACAATTTTTCATAAATCCTACTTTGTCTTTCGTCACAACGTGTCGAGGTGATGGTTATTCTTTTATGTACCCGTGTAAGCTAGACCATATTTGAGTAGTACATTATGATCTAACAGCCCATCCAACTAGGATTGAACTCATGACCTCTTAGCCCTTTGTCAGGATCATTCCTCCTATCTAATAAGCCGACCAATGATAGTTTACAAAGAAAATCTAATAAGTTAAAAAATCAGAACCAAATTTGTAATTATATGGTCCCCAATTTTAGTTTTTTGAACTGAGACACAAAAAAATTGAAAGAGACCGTCAAGAGAGTGTTTAAGACGTCGAGTTGAGTTTTGAAATATGAAGTTAATATGATGGAGTTAGGTAGTTTGTGTTTGG

mRNA sequence

TTTTAATTCTAAACCGTACAAGTGACTCAGCCTTGGACGTCCCGTATAAATGTAGAAGATCCAGAGAACGGAAGATAGACGGAGAAAAACAGAGAAATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGATTGAATTATATAGATTAAGAAAACCATGTTTGACTTTCAGCATCAGTAAATATAATACGGCCTATTTAGTAACAATAATTTTGTTGTTAGTTTTCTCAATTTCCCCTTTGTAATCTGTGCTATACTATATATAAAAAGGGTTATGAGTGTAAAATCTTTGTTTCCCATCTTAGACCTCACAATTTTTCATAAATCCTACTTTGTCTTTCGTCACAACGTGTCGAGGTGATGGTTATTCTTTTATGTACCCGTGTAAGCTAGACCATATTTGAGTAGTACATTATGATCTAACAGCCCATCCAACTAGGATTGAACTCATGACCTCTTAGCCCTTTGTCAGGATCATTCCTCCTATCTAATAAGCCGACCAATGATAGTTTACAAAGAAAATCTAATAAGTTAAAAAATCAGAACCAAATTTGTAATTATATGGTCCCCAATTTTAGTTTTTTGAACTGAGACACAAAAAAATTGAAAGAGACCGTCAAGAGAGTGTTTAAGACGTCGAGTTGAGTTTTGAAATATGAAGTTAATATGATGGAGTTAGGTAGTTTGTGTTTGG

Coding sequence (CDS)

ATGCAGTGCTCCGATTTTACCTTATCAGGGAGCGATTCACGCTTCTACATCCCTTCGAACCCTAGCACTACCGGCCGCCGACCAATTTCCGGCCACCGATCTCGGATATTCTTTCCCAACACCCTGCCGTGCAATTCGACGCGCTTGCCGTCTCTTTCAATCAGAGCCAAGGCCTCGTACAACGAAGGAATCCTCTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGTATATCTCAGTCCGGATCCTTGGCGGAAATCAAGCACGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGTGCGGAGGAGTATACGAAGACGTTCATTCGTGTTCAGGAGGCGTACGAGACATTGTCGGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGGTTGGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGACGCCGATGAGGAAGTTCCAGAGAGAAGTGGATGGAAAAATTGCTGGCAAGCTCAGATCTCAGAATTGAAGAGAAGAAGCATGGACAAGGATTCGAGACAAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

Protein sequence

MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNEQV

Homology

[Close]

BLAST of Lsi05G018600.1 vs. Swiss-Prot
Match: DNJ20_ARATH (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 7.8e-42
Identity = 95/158 (60.13%), Postives = 114/158 (72.15%), Query Frame = 1


Query: 47  TRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSP 106
           TR  S  I+++ ++++ +  SE  + SFYDLLG+++S +L EIK AYKQLARKYHPDVSP
Sbjct: 42  TRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 101

Query: 107 PGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARR--RYDADEEVPERS 166
           P R EEYT  FIRVQEAYETLSDPRRR LYDRD+  G   +FS RR  RYD  E V E+S
Sbjct: 102 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYD-QEVVEEKS 161

Query: 167 GWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
            WK  WQ Q+S L+RRS  KD+   MSW ARMRRQ  E
Sbjct: 162 EWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

[Close]

BLAST of Lsi05G018600.1 vs. Swiss-Prot
Match: DNAJ_THEAB (Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-12
Identity = 35/70 (50.00%), Postives = 50/70 (71.43%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +A+  +Y++LG+S++ S  EI+ AYKQL +K+HPD +   R E   K F  +QEAYE LS
Sbjct: 1   MAKKDYYEILGVSRNASQEEIRQAYKQLIKKWHPDRNHQNRKEAEEK-FKEIQEAYEVLS 60

Query: 129 DPRRRALYDR 139
           DP +RA+YDR
Sbjct: 61  DPEKRAMYDR 69

[Close]

BLAST of Lsi05G018600.1 vs. Swiss-Prot
Match: DNAJ_LACRD (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/79 (49.37%), Postives = 53/79 (67.09%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE  +YD+LG+S+  S  +IK AY++LA KYHPDV+    AEE    F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]

BLAST of Lsi05G018600.1 vs. Swiss-Prot
Match: DNAJ_LACRJ (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/79 (49.37%), Postives = 53/79 (67.09%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE  +YD+LG+S+  S  +IK AY++LA KYHPDV+    AEE    F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQVA 148
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]

BLAST of Lsi05G018600.1 vs. Swiss-Prot
Match: DNAJ_LACF3 (Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-12
Identity = 39/77 (50.65%), Postives = 51/77 (66.23%), Query Frame = 1


Query: 69  VAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLS 128
           +AE   YD+LG+ +  S AEIK AY++LA KYHPDV+    AE   K F ++ EAYETLS
Sbjct: 1   MAEEDLYDVLGVKKDASEAEIKRAYRKLAAKYHPDVNHEPGAE---KKFKKINEAYETLS 60

Query: 129 DPRRRALYDRDMVGGLQ 146
           D ++RA YD+    G Q
Sbjct: 61  DDQKRAQYDQFGTTGSQ 74

[Close]

BLAST of Lsi05G018600.1 vs. TrEMBL
Match: A0A0A0L4L9_CUCSA (Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus GN=Csa_3G129700 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 2.6e-92
Identity = 172/203 (84.73%), Postives = 186/203 (91.63%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSD T SGSDSRFYIPSNP+TT RRPISG+RSR+ FP+T P NSTRLPSLSIRAKAS+
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPG  EE TK FIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYDADEEV ++SGW+N W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDKDSRQNMSWGARMRRQMNEQ 204
           SM+KD R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]

BLAST of Lsi05G018600.1 vs. TrEMBL
Match: W9S291_9ROSA (Chaperone protein dnaJ 20 OS=Morus notabilis GN=L484_011075 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 3.7e-59
Identity = 125/203 (61.58%), Postives = 151/203 (74.38%), Query Frame = 1


Query: 1   MQCSDFTLS-GSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C+   +S GSDSRF+ P+N ST          S + FP   P  STR+ +     +AS
Sbjct: 1   MRCNGLAISGGSDSRFFFPTNSSTIYTASARNPSSTVLFPGR-PVFSTRIRA---TPRAS 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
               ++   VAE SFY+LLGI +SGSL EIK AYKQLARKYHPDVSPPGR EEYT+TFIR
Sbjct: 61  AAAAVVGDRVAELSFYELLGIPESGSLGEIKQAYKQLARKYHPDVSPPGRVEEYTRTFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPR RA+YDRDM  GL +AFSARRRY +DE + E+S WK+ WQ+Q+SELKR
Sbjct: 121 VQEAYETLSDPRLRAIYDRDMAKGLHLAFSARRRYQSDEGMEEKSEWKSHWQSQLSELKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS++KDSR NMSWGARMRR+M +
Sbjct: 181 RSVNKDSRGNMSWGARMRRRMED 199

[Close]

BLAST of Lsi05G018600.1 vs. TrEMBL
Match: A0A061EJK6_THECC (Chaperone protein dnaJ 20 OS=Theobroma cacao GN=TCM_020242 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 6.8e-53
Identity = 115/200 (57.50%), Postives = 140/200 (70.00%), Query Frame = 1


Query: 3   CSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNE 62
           C    + G+D+RF++P       RRP+               + TR+   S++ KA  NE
Sbjct: 5   CGLAAIPGTDARFFVPDTSFIRLRRPV---------------DPTRVQFGSLKIKARINE 64

Query: 63  GILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQE 122
                   E SFY+LLGI ++G+L EIK AYKQLARKYHPDVSPP R EEYT+ FIRVQE
Sbjct: 65  AR-----KEVSFYELLGIPETGTLPEIKQAYKQLARKYHPDVSPPDRVEEYTQRFIRVQE 124

Query: 123 AYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRRSM 182
           AYETLSDPRRRA YDRD+  G+ +AFSARRRY  DEE+ ERS WKN WQ+Q+SELKRRSM
Sbjct: 125 AYETLSDPRRRASYDRDLALGIHLAFSARRRYQYDEELEERSEWKNRWQSQLSELKRRSM 184

Query: 183 DKDSRQNMSWGARMRRQMNE 203
           ++D+R NMSWGARMRRQ +E
Sbjct: 185 NRDARGNMSWGARMRRQRDE 184

[Close]

BLAST of Lsi05G018600.1 vs. TrEMBL
Match: B9RWC0_RICCO (Chaperone protein dnaJ 20, chloroplast, putative OS=Ricinus communis GN=RCOM_1017840 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.6e-52
Identity = 114/199 (57.29%), Postives = 138/199 (69.35%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           M C++  L G     Y     S++   PI+  +   F     P   TR P  S + KA+ 
Sbjct: 1   MHCTNLMLPGGSDTVYFFHPSSSSSTTPITKSKPGPFL-RLKPV--TRFPMSSFKTKAAV 60

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           ++ I + ++   SFY+LLGI +SGSL +IK AYKQLARKYHPDVSPP R  EYT+ FI+V
Sbjct: 61  SDRIATKDL---SFYELLGIPESGSLIDIKQAYKQLARKYHPDVSPPDRVHEYTQRFIQV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSDPRRRA YDRDM  GL +AFSARRRY +DEEV   S WKN W+AQ+SELKRR
Sbjct: 121 QEAYETLSDPRRRATYDRDMARGLHLAFSARRRYQSDEEVEVSSDWKNRWEAQLSELKRR 180

Query: 181 SMDKDSRQNMSWGARMRRQ 200
           SM+KDS +NMSW ARMRRQ
Sbjct: 181 SMNKDSEENMSWAARMRRQ 193

[Close]

BLAST of Lsi05G018600.1 vs. TrEMBL
Match: A0A059B1U7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H02689 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 3.8e-51
Identity = 116/203 (57.14%), Postives = 138/203 (67.98%), Query Frame = 1


Query: 1   MQCSDFTLSGSD-SRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C    L G D SR  +P  P         GHR+  F P  +   + R  +  I   A 
Sbjct: 1   MRCYGVALPGGDPSRLCLPPPPPPFPSARTHGHRA--FLPTRVRFGAVRCAAGRIDDGAV 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
            +    S+  AE SFYDLLGI +SG+L EIK AYKQLARKYHPDVSPPGR EEYT+ FIR
Sbjct: 61  ADAARCSA--AEASFYDLLGIPESGTLPEIKQAYKQLARKYHPDVSPPGRVEEYTQRFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPRRRA+YDRD+  G+ +AFSARR Y  DE++ ++S WK+ WQ Q+S LKR
Sbjct: 121 VQEAYETLSDPRRRAVYDRDLARGIHLAFSARRPYRGDEDLEDKSEWKSRWQVQLSGLKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS  KDSR NMSWGARMR+QM E
Sbjct: 181 RSAHKDSRGNMSWGARMRKQMEE 199

[Close]

BLAST of Lsi05G018600.1 vs. TAIR10
Match: AT4G13830.2 (AT4G13830.2 DNAJ-like 20)

HSP 1 Score: 171.8 bits (434), Expect = 4.4e-43
Identity = 95/158 (60.13%), Postives = 114/158 (72.15%), Query Frame = 1


Query: 47  TRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSP 106
           TR  S  I+++ ++++ +  SE  + SFYDLLG+++S +L EIK AYKQLARKYHPDVSP
Sbjct: 42  TRFSSTRIQSRLTHDDPVKQSE--DLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSP 101

Query: 107 PGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARR--RYDADEEVPERS 166
           P R EEYT  FIRVQEAYETLSDPRRR LYDRD+  G   +FS RR  RYD  E V E+S
Sbjct: 102 PDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYD-QEVVEEKS 161

Query: 167 GWKNCWQAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
            WK  WQ Q+S L+RRS  KD+   MSW ARMRRQ  E
Sbjct: 162 EWKAKWQTQLSGLRRRSNQKDN-NTMSWAARMRRQQQE 195

[Close]

BLAST of Lsi05G018600.1 vs. TAIR10
Match: AT4G39960.1 (AT4G39960.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 71.6 bits (174), Expect = 6.2e-13
Identity = 43/119 (36.13%), Postives = 63/119 (52.94%), Query Frame = 1


Query: 29  ISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAE 88
           +   RS +F   +LP     L    +        G   +  A+  FY +LG+S++ + AE
Sbjct: 45  LGASRSSMFAQGSLPF----LSLTGVSPNTHSRRGARFTVRADTDFYSVLGVSKNATKAE 104

Query: 89  IKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVA 148
           IK AY++LAR YHPDV+    AE+    F  +  AYE LSD  +R+LYDR    G++ A
Sbjct: 105 IKSAYRKLARSYHPDVNKDAGAED---KFKEISNAYEILSDDEKRSLYDRYGEAGVKGA 156

[Close]

BLAST of Lsi05G018600.1 vs. TAIR10
Match: AT2G22360.1 (AT2G22360.1 DNAJ heat shock family protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-12
Identity = 36/78 (46.15%), Postives = 51/78 (65.38%), Query Frame = 1


Query: 70  AEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSD 129
           A+  +Y +LG+S++ + AEIK AY++LAR YHPDV+    AEE    F  +  AYE LSD
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 130 PRRRALYDRDMVGGLQVA 148
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]

BLAST of Lsi05G018600.1 vs. TAIR10
Match: AT3G17830.1 (AT3G17830.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 67.0 bits (162), Expect = 1.5e-11
Identity = 46/134 (34.33%), Postives = 71/134 (52.99%), Query Frame = 1


Query: 17  IPS-NPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASYNEGILSSEVAEGSFY 76
           IPS NP+ + R       +  + P TLP  S R+    I     Y   +++   A    Y
Sbjct: 10  IPSINPAHSFRHQFPQPNASFYLPPTLPIFS-RVRRFGI--SGGYRRRVITM-AAGTDHY 69

Query: 77  DLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRVQEAYETLSDPRRRAL 136
             L ++++ +L EIK +Y++LARKYHPD++    AE+    F ++  AYE LSD  +R+ 
Sbjct: 70  STLNVNRNATLQEIKSSYRKLARKYHPDMNKNPGAED---KFKQISAAYEVLSDEEKRSA 129

Query: 137 YDRDMVGGLQVAFS 150
           YDR    GL+  F+
Sbjct: 130 YDRFGEAGLEGDFN 136

[Close]

BLAST of Lsi05G018600.1 vs. TAIR10
Match: AT1G80030.1 (AT1G80030.1 Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 65.5 bits (158), Expect = 4.4e-11
Identity = 34/68 (50.00%), Postives = 47/68 (69.12%), Query Frame = 1


Query: 72  GSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVS-PPGRAEEYTKTFIRVQEAYETLSDP 131
           G +Y  LG+S+S +  EIK AY++LAR+YHPDV+  PG  E+    F  +  AYE LSD 
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGATEK----FKEISAAYEVLSDE 133

Query: 132 RRRALYDR 139
           ++RALYD+
Sbjct: 134 QKRALYDQ 137

[Close]

BLAST of Lsi05G018600.1 vs. NCBI nr
Match: gi|659075782|ref|XP_008438327.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo])

HSP 1 Score: 359.8 bits (922), Expect = 3.2e-96
Identity = 178/204 (87.25%), Postives = 191/204 (93.63%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSDFT SGSDSRFYIPSNP+ + RRPISG+RSR+FFP+T P NSTRLPSLSIRAKAS+
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGISQSGSLAEIK AYKQLARKYHPDVSPPG AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSDPRRRALYDRDM+GGLQVAFSARRRYDA EEV E+SGW+N W+AQISELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMDKDSRQNMSWGARMRRQMNEQV 205
           SM+KD RQNMSWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

[Close]

BLAST of Lsi05G018600.1 vs. NCBI nr
Match: gi|449432390|ref|XP_004133982.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 346.3 bits (887), Expect = 3.7e-92
Identity = 172/203 (84.73%), Postives = 186/203 (91.63%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQCSD T SGSDSRFYIPSNP+TT RRPISG+RSR+ FP+T P NSTRLPSLSIRAKAS+
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
           NEG +SSEVAEGSFYDLLGIS+SGSL EIK AYKQLARKYHPDVSPPG  EE TK FIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYDADEEV ++SGW+N W+AQISELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDKDSRQNMSWGARMRRQMNEQ 204
           SM+KD R NMSWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]

BLAST of Lsi05G018600.1 vs. NCBI nr
Match: gi|703148387|ref|XP_010109333.1| (Chaperone protein dnaJ 20 [Morus notabilis])

HSP 1 Score: 236.1 bits (601), Expect = 5.4e-59
Identity = 125/203 (61.58%), Postives = 151/203 (74.38%), Query Frame = 1


Query: 1   MQCSDFTLS-GSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKAS 60
           M+C+   +S GSDSRF+ P+N ST          S + FP   P  STR+ +     +AS
Sbjct: 1   MRCNGLAISGGSDSRFFFPTNSSTIYTASARNPSSTVLFPGR-PVFSTRIRA---TPRAS 60

Query: 61  YNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIR 120
               ++   VAE SFY+LLGI +SGSL EIK AYKQLARKYHPDVSPPGR EEYT+TFIR
Sbjct: 61  AAAAVVGDRVAELSFYELLGIPESGSLGEIKQAYKQLARKYHPDVSPPGRVEEYTRTFIR 120

Query: 121 VQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKR 180
           VQEAYETLSDPR RA+YDRDM  GL +AFSARRRY +DE + E+S WK+ WQ+Q+SELKR
Sbjct: 121 VQEAYETLSDPRLRAIYDRDMAKGLHLAFSARRRYQSDEGMEEKSEWKSHWQSQLSELKR 180

Query: 181 RSMDKDSRQNMSWGARMRRQMNE 203
           RS++KDSR NMSWGARMRR+M +
Sbjct: 181 RSVNKDSRGNMSWGARMRRRMED 199

[Close]

BLAST of Lsi05G018600.1 vs. NCBI nr
Match: gi|1009139653|ref|XP_015887231.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 218.8 bits (556), Expect = 8.9e-54
Identity = 122/212 (57.55%), Postives = 144/212 (67.92%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYI----PSNPSTTGRR------PISGHRSRIFFPNTLPCNSTRLP 60
           M+    T+SGSDSRF+     P N S           P   HR  IF   T     TR  
Sbjct: 1   MRSHGLTISGSDSRFFSFPVKPVNKSIPDPNHNVLFFPAHHHRPIIFTTTT-----TRDS 60

Query: 61  SLSIRAKASYNEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRA 120
             ++R KA+ N+G + SE AE SFY+LLGI + GSL +IK AYKQLARKYHPDVSPPGR 
Sbjct: 61  IGTVRVKATVNDGFVFSEAAELSFYELLGIPEWGSLVDIKQAYKQLARKYHPDVSPPGRV 120

Query: 121 EEYTKTFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCW 180
           EEYT+ FIRVQEAYETLSDPRRRALYDRDM  G+ +AFSAR+RY  DE + ++  WK  W
Sbjct: 121 EEYTQRFIRVQEAYETLSDPRRRALYDRDMAKGIHLAFSARKRYQKDEGMEDKGEWKYRW 180

Query: 181 QAQISELKRRSMDKDSRQNMSWGARMRRQMNE 203
           Q+Q+SELKRRS +KD   NMSWGARMRR+  E
Sbjct: 181 QSQLSELKRRSTNKDMTGNMSWGARMRRRREE 207

[Close]

BLAST of Lsi05G018600.1 vs. NCBI nr
Match: gi|470121999|ref|XP_004297043.1| (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 216.1 bits (549), Expect = 5.8e-53
Identity = 120/199 (60.30%), Postives = 142/199 (71.36%), Query Frame = 1


Query: 1   MQCSDFTLSGSDSRFYIPSNPSTTGRRPISGHRSRIFFPNTLPCNSTRLPSLSIRAKASY 60
           MQ    T+ G+DSR YI      +  RP    +     P++LP  S+R    SI+AKA+ 
Sbjct: 1   MQTYGLTIPGADSRLYITP---ISAARPAPDPK-----PSSLPVFSSRTRFGSIKAKAAT 60

Query: 61  NEGILSSEVAEGSFYDLLGISQSGSLAEIKHAYKQLARKYHPDVSPPGRAEEYTKTFIRV 120
             G +++E  E SFY+LLGI +SGS+ EIK AYKQLARKYHPDVSPPGR EEYT+ FIRV
Sbjct: 61  MNGAVTAEEKELSFYELLGIPESGSIKEIKQAYKQLARKYHPDVSPPGRVEEYTEKFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDADEEVPERSGWKNCWQAQISELKRR 180
           QEAYETLSD RRRALYD+DM  GL +AFSA RRY  DE +  +  WKN WQ+Q+SELKRR
Sbjct: 121 QEAYETLSDSRRRALYDQDMARGLHLAFSA-RRYQYDEGMEVKGDWKNRWQSQLSELKRR 180

Query: 181 SMDKDSRQNMSWGARMRRQ 200
           S  KD RQNMSWGARMRRQ
Sbjct: 181 SSYKDDRQNMSWGARMRRQ 190

The following BLAST results are available for this feature:

BLAST of Lsi05G018600.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DNJ20_ARATH	7.8e-42	60.13	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana GN=ATJ20 PE=1 S...	[more]
DNAJ_THEAB	2.9e-12	50.00	Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) GN=dnaJ PE=3 SV=...	[more]
DNAJ_LACRD	3.8e-12	49.37	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) GN=dnaJ PE=3 ...	[more]
DNAJ_LACRJ	3.8e-12	49.37	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) GN=dnaJ PE=3 S...	[more]
DNAJ_LACF3	3.8e-12	50.65	Chaperone protein DnaJ OS=Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)...	[more]

BLAST of Lsi05G018600.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4L9_CUCSA	2.6e-92	84.73	Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus GN=Csa_3G129700 PE=4 S...	[more]
W9S291_9ROSA	3.7e-59	61.58	Chaperone protein dnaJ 20 OS=Morus notabilis GN=L484_011075 PE=4 SV=1	[more]
A0A061EJK6_THECC	6.8e-53	57.50	Chaperone protein dnaJ 20 OS=Theobroma cacao GN=TCM_020242 PE=4 SV=1	[more]
B9RWC0_RICCO	7.6e-52	57.29	Chaperone protein dnaJ 20, chloroplast, putative OS=Ricinus communis GN=RCOM_101...	[more]
A0A059B1U7_EUCGR	3.8e-51	57.14	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H02689 PE=4 SV=1	[more]

BLAST of Lsi05G018600.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G13830.2	4.4e-43	60.13	DNAJ-like 20	[more]
AT4G39960.1	6.2e-13	36.13	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT2G22360.1	1.4e-12	46.15	DNAJ heat shock family protein	[more]
AT3G17830.1	1.5e-11	34.33	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.1	4.4e-11	50.00	Molecular chaperone Hsp40/DnaJ family protein	[more]

BLAST of Lsi05G018600.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659075782\|ref\|XP_008438327.1\|	3.2e-96	87.25	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo]	[more]
gi\|449432390\|ref\|XP_004133982.1\|	3.7e-92	84.73	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis sativus]	[more]
gi\|703148387\|ref\|XP_010109333.1\|	5.4e-59	61.58	Chaperone protein dnaJ 20 [Morus notabilis]	[more]
gi\|1009139653\|ref\|XP_015887231.1\|	8.9e-54	57.55	PREDICTED: chaperone protein dnaJ 20, chloroplastic [Ziziphus jujuba]	[more]
gi\|470121999\|ref\|XP_004297043.1\|	5.8e-53	60.30	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Fragaria vesca subsp. ...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR018253	DnaJ_domain_CS
IPR001623	DnaJ_domain

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi05G018600	Lsi05G018600	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G018600.1.five_prime_UTR.1	Lsi05G018600.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G018600.1.CDS.1	Lsi05G018600.1.CDS.1	CDS
Lsi05G018600.1.CDS.2	Lsi05G018600.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G018600.1.three_prime_UTR.1	Lsi05G018600.1.three_prime_UTR.1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi05G018600.1	Lsi05G018600.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 112..132 score: 7.7E-16coord: 132..151 score: 7.7E-16coord: 75..93 score: 7.7E-16coord: 93..108 score: 7.7
IPR001623	DnaJ domain	GENE3D	G3DSA:1.10.287.110		coord: 67..144 score: 2.1
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 74..137 score: 2.2
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 72..132 score: 4.2
IPR001623	DnaJ domain	PROFILE	PS50076	DNAJ_2	coord: 73..140 score: 19
IPR001623	DnaJ domain	unknown	SSF46565	Chaperone J-domain	coord: 73..146 score: 1.96
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 117..136 scor
None	No IPR available	PANTHER	PTHR24078	DNAJ HOMOLOG SUBFAMILY C MEMBER	coord: 74..138 score: 1.0
None	No IPR available	PANTHER	PTHR24078:SF222	SUBFAMILY NOT NAMED	coord: 74..138 score: 1.0