CmaCh02G004450.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh02G004450.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Carboxylesterase 1
Location	Cma_Chr02 : 2275085 .. 2276053 (+)
Sequence length	969

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGTGTGGAGTCTAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCGGGCTCTGGGAAAGGAGATCAGAAGCTTCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTCTACCACCAGCACCTCAACCCTTGGGTGGCTGAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGGCGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGACTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCACATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATACATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAACCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGACAATCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTCGATGTGGTGGAGACCGACGGCGAAGGGCACGTCTTCCATTTGTTCAATCCAACCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCCGTTGTTGA

mRNA sequence

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGTGTGGAGTCTAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCGGGCTCTGGGAAAGGAGATCAGAAGCTTCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTCTACCACCAGCACCTCAACCCTTGGGTGGCTGAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGGCGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGACTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCACATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATACATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAACCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGACAATCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTCGATGTGGTGGAGACCGACGGCGAAGGGCACGTCTTCCATTTGTTCAATCCAACCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCCGTTGTTGA

Coding sequence (CDS)

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGTGTGGAGTCTAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCGGGCTCTGGGAAAGGAGATCAGAAGCTTCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTCTACCACCAGCACCTCAACCCTTGGGTGGCTGAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGGCGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGACTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCACATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATACATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAACCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGACAATCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTCGATGTGGTGGAGACCGACGGCGAAGGGCACGTCTTCCATTTGTTCAATCCAACCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCCGTTGTTGA

Protein sequence

MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTCDSAADLVGQLAAFISGKCRC

Homology

[Close]

BLAST of CmaCh02G004450.1 vs. Swiss-Prot
Match: CXE12_ARATH (Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.0e-85
Identity = 160/321 (49.84%), Postives = 209/321 (65.11%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA++ +P   IYK  RI+RL+G    PP  +P+ GV SKDVV S +++++VRIY P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
           A   + D KLPLLVYFHGGGF IETAFSP YH  L   V+ +N VAVSV+YRRAPEH + 
Sbjct: 64  AA--ETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE--- 184
           +PF+DSWTALKW+  H  G+G ++WLN+ AD ++V+L+GDSAG NI H MA+R A E   
Sbjct: 124 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 183

Query: 185 -GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVN- 244
            GL    I G+ L+HP+FW    I E+   D      +E  W   S + KD  DDP++N 
Sbjct: 184 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 243

Query: 245 -PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHL 304
                 DL  L   KV + VAEKD L  +G  YA  L KSGW G V+VVE++GE HVFHL
Sbjct: 244 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 303

Query: 305 FNPTCDSAADLVGQLAAFISG 319
             P CD+A +++ + + FI G
Sbjct: 304 LKPECDNAIEVMHKFSGFIKG 322

[Close]

BLAST of CmaCh02G004450.1 vs. Swiss-Prot
Match: CXE13_ARATH (Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.9e-79
Identity = 154/324 (47.53%), Postives = 207/324 (63.89%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP-KS 64
           EIA + +P  IIYK  RI+RL+G    PP  +P+ GV SKDVV S ++++++RIY P K+
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQL 124
           A    +   KLPLLVYFHGGGF +ETAFSP YH  L   V+ ++ VAVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMAL-----RM 184
           P  ++DSWTALKW+ +H  G+G ++WLN+ AD ++V+LAGDSAG NI H M +     ++
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 ATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIV 244
           + E L    I G+ L+HP+FW    + +++  D      +E++W   S + KD  DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTV-DVVETDGEGHV 304
           N       DL  L   KV + VAEKD L  +G  Y E L KS W G V DVVET GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPTCDSAADLVGQLAAFISG 319
           FHL +P  + A +LV + A FI G
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIKG 327

[Close]

BLAST of CmaCh02G004450.1 vs. Swiss-Prot
Match: CXE2_ARATH (Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.5e-79
Identity = 149/315 (47.30%), Postives = 204/315 (64.76%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           +++L   PW +++ D  ++RL G ++ PPGLDP TGV SKD++I  +  ++ RIYRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
               +  QK+PL++YFHGG F I +   P YH  LN  V +AN++AVSVNYR APEH LP
Sbjct: 67  ----QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLP 126

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +EDSWTALK I A +     + W+N+ ADL+ ++L GDSAG NI+H +A R A +   
Sbjct: 127 TAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQ 186

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNP--EHE 244
            +KIKG+ +IHP+FWG + IG E + D     +V+  W +V    K  DDP +NP  +  
Sbjct: 187 TLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 246

Query: 245 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 304
           PDLG L  E+V I VAEKD L ERG+ Y E L KS W G V+++ET  + HVFH+F P C
Sbjct: 247 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 306

Query: 305 DSAADLVGQLAAFIS 318
           D A ++V  LA FI+
Sbjct: 307 DEAMEMVRCLALFIN 310

[Close]

BLAST of CmaCh02G004450.1 vs. Swiss-Prot
Match: CXE7_ARATH (Probable carboxylesterase 7 OS=Arabidopsis thaliana GN=CXE7 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.2e-78
Identity = 149/313 (47.60%), Postives = 195/313 (62.30%), Query Frame = 1


Query: 6   IALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAA 65
           IA + +P F +YK  RI+RL+G    PP L P+ GV SKD++ S E ++++RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  GSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLPI 125
                 +KLP+L+YFHGGGF IETAFSP YH  L   VA AN +A+SVNYRRAPE  +PI
Sbjct: 65  V-----KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPI 124

Query: 126 PFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAG 185
           P+EDSW +LKW+  H  GTGP+ W+N+  D  +V+LAGDSAGGNI+H + +R   E L  
Sbjct: 125 PYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCD 184

Query: 186 VKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVNPEHEPD 245
             I G+ LIHP+FW    I E +  D      VE  W   S + K  +DDP +N      
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 246 LGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTCDS 305
            G L   +V + VA  D    +G  YAE L+KSGW G V+V+ET  EGHVFHL NP  D+
Sbjct: 245 SG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDN 304

Query: 306 AADLVGQLAAFIS 318
           A  +V +L  FI+
Sbjct: 305 ARQVVKKLEEFIN 311

[Close]

BLAST of CmaCh02G004450.1 vs. Swiss-Prot
Match: CXE5_ARATH (Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-74
Identity = 141/317 (44.48%), Postives = 192/317 (60.57%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA    P+  IYKD R++RLIG D  P  LDP   V SKDV+ S E++++VR++ P  +
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
                G+ KLPLL+Y HGG + IE+ FSP YH +L   V  AN +AVSV YRRAPE  +P
Sbjct: 64  TKLTAGN-KLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +ED W+A++WI AHSNG+GP +W+N+ AD  +V+L GDSAGGNI+H MA++   E   
Sbjct: 124 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 183

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVS--KDIKDLDDPIVNPEHE 244
            +KIKG+ ++HP FWG   + E    D ++   +  +W  ++    +   DDP+ N    
Sbjct: 184 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 243

Query: 245 -PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 304
             D   L  +KV + VA KD    +G  YA  L K  W GTV+VVE +GE HVFHL NP 
Sbjct: 244 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 303

Query: 305 CDSAADLVGQLAAFISG 319
            D A   + +   FI G
Sbjct: 304 SDKALKFLKKFVEFIIG 319

[Close]

BLAST of CmaCh02G004450.1 vs. TrEMBL
Match: A0A0A0LKY5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234510 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.2e-157
Identity = 263/321 (81.93%), Postives = 288/321 (89.72%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALN AP+FI+YKD RIDRLIGNDIDPPGLDPKTGVE+KDV IS +  VAVR+YR
Sbjct: 1   MASDDIALNVAPFFILYKDGRIDRLIGNDIDPPGLDPKTGVETKDVDISPD--VAVRVYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PKS     K  +KLPLLVYFHGGGFCIETAFSPFY+QH++ WVAEANI AVSVNYRRAPE
Sbjct: 61  PKSP--DEKQSEKLPLLVYFHGGGFCIETAFSPFYNQHISAWVAEANIAAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+WTA+KWIA+HS G GPDEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTAMKWIASHSEGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGG+L+GEE +WDPK + VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGELLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGRL AE+V IYVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRLPAERVGIYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           TCD A +LV QLAAFI   CR
Sbjct: 301 TCDMAGELVKQLAAFIKSGCR 317

[Close]

BLAST of CmaCh02G004450.1 vs. TrEMBL
Match: A0A0A0LIH8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234520 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 9.9e-123
Identity = 204/318 (64.15%), Postives = 250/318 (78.62%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MAS EIA N +P  I+YKD R +RL+GN++  P LDP T VESKD+VIS E  V+ RIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PK  A       KLPLL+Y HGGGFCIE+AFSP YH HLN  VAEAN++A+SV YRRAPE
Sbjct: 61  PKPTAEP----HKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           H LPI +EDSWTALKW+AAHS GTGP+EWLN+IAD N+VY AGDSAG N+A++MA+R+  
Sbjct: 121 HPLPIAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGM 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EG+AG+ +KGL L+HP+FWG KLIG+E++  P+    +E LWY     I  LDDPIVNPE
Sbjct: 181 EGVAGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
            EP+LG++ AE+VA+YVAEKD LK+RGR Y+ECL+KSGWGG V+V ET G+GHVFHLFNP
Sbjct: 241 FEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISG 319
           T D A   VG+LAAF++G
Sbjct: 301 TSDDAVQFVGKLAAFLNG 314

[Close]

BLAST of CmaCh02G004450.1 vs. TrEMBL
Match: V7CGY0_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G295200g PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.7e-102
Identity = 175/315 (55.56%), Postives = 231/315 (73.33%), Query Frame = 1


Query: 3   SDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPK 62
           S E+A++ +P+  IYKD  I+R+ GNDI PP LDP T VESKDVVIS++D V+ RI+ PK
Sbjct: 5   STEVAMDLSPFVKIYKDGHIERVTGNDIVPPCLDPATNVESKDVVISKDDDVSARIFIPK 64

Query: 63  SAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQ 122
               +    QKLPLLVYFHGGGFCIET +SP YH+ +N  V++ANIVAVSV+YRRAPEH 
Sbjct: 65  LTDQT----QKLPLLVYFHGGGFCIETPYSPPYHKFINSLVSKANIVAVSVHYRRAPEHP 124

Query: 123 LPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEG 182
           +PI  EDSWT+LKW+ +H +G GP+EWLN  AD  +V+  GDSAG NI+H MALR+   G
Sbjct: 125 VPIAHEDSWTSLKWVVSHFDGNGPEEWLNRHADFGKVFCGGDSAGANISHHMALRIGIHG 184

Query: 183 LAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEHE 242
           L G+ ++G+ L+HP+FWG + IG E +   + + +VENLW + S      DDP++NP  +
Sbjct: 185 LPGMNLQGIVLVHPYFWGVERIGSEAQ---EHVPIVENLWRFTSPASTGSDDPLLNPAKD 244

Query: 243 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 302
           PDLG+L  ++V + VAEKD LK+RG HY E L KS W G  +V+E +GEGHVFHLFNP C
Sbjct: 245 PDLGKLGCKRVLVCVAEKDFLKDRGWHYKELLEKSEWEGVAEVLEANGEGHVFHLFNPDC 304

Query: 303 DSAADLVGQLAAFIS 318
           D+A  L+ Q+A+FI+
Sbjct: 305 DNAVSLLDQIASFIN 312

[Close]

BLAST of CmaCh02G004450.1 vs. TrEMBL
Match: G7JMF3_MEDTR (CXE carboxylesterase OS=Medicago truncatula GN=MTR_4g086510 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.6e-99
Identity = 165/316 (52.22%), Postives = 229/316 (72.47%), Query Frame = 1


Query: 2   ASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP 61
           +S+E+ L+ +P   IYKD  I+RLIG+DI PP  DP T VESKD++IS++ +++ RI+ P
Sbjct: 4   SSNEVVLDLSPMIKIYKDGHIERLIGSDIVPPSFDPTTNVESKDILISKDQNISARIFIP 63

Query: 62  KSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEH 121
           K        +QKLPLLVYFHGGGFC+ET FSP YH  LN  V++AN++AVSV+YRRAPEH
Sbjct: 64  K-LNNDQFPNQKLPLLVYFHGGGFCVETPFSPPYHNFLNTIVSKANVIAVSVDYRRAPEH 123

Query: 122 QLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE 181
            LPI +EDSWT+LKW+ +H +G G DEW+N  AD  +++ AGDSAG NIA+ MA+R+ T+
Sbjct: 124 PLPIAYEDSWTSLKWVVSHLHGNGSDEWINRYADFGKMFFAGDSAGANIANHMAIRVGTQ 183

Query: 182 GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEH 241
           GL G+ ++G+ L+H  FWG + +G E     + + + +NLW +V       DDP +NP  
Sbjct: 184 GLQGINLEGIVLVHTFFWGVERVGSEATEKSEHLSLADNLWRFVCPTSSGSDDPFLNPGK 243

Query: 242 EPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 301
           + +LGRL  ++V + VAE D+LK+RG +Y E L K G+GG V+V+ET GEGHVFHLFNP 
Sbjct: 244 DKNLGRLGCKRVLVCVAENDSLKDRGWYYKELLEKIGYGGVVEVIETKGEGHVFHLFNPN 303

Query: 302 CDSAADLVGQLAAFIS 318
           CD+A  L+ Q+A+FI+
Sbjct: 304 CDNAISLLNQIASFIN 318

[Close]

BLAST of CmaCh02G004450.1 vs. TrEMBL
Match: B9N4W8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s14970g PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.2e-98
Identity = 168/317 (53.00%), Postives = 221/317 (69.72%), Query Frame = 1


Query: 2   ASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP 61
           A  + AL+ +P+ I+YKD  I+RL+GN+I PP LDPK+ V SKD V S+E  ++ R+Y P
Sbjct: 4   AEPDAALDLSPFIIVYKDGSIERLVGNEIVPPSLDPKSSVLSKDAVYSKEAKLSSRLYLP 63

Query: 62  KSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEH 121
                    D+KLPLL+YF+GGGFC+E+AFSP YH +LN  VAEA ++AVSV+YRR PEH
Sbjct: 64  PGV----DPDKKLPLLIYFYGGGFCVESAFSPAYHNYLNILVAEAKVIAVSVDYRRVPEH 123

Query: 122 QLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE 181
            +P+P++DSWTALKW+A+H NG GP++WLN  AD  +VYLAGDSAGGNIAH MA+R   E
Sbjct: 124 PIPVPYDDSWTALKWVASHVNGDGPEKWLNNHADFGKVYLAGDSAGGNIAHHMAMRYGQE 183

Query: 182 GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEH 241
            L GVK  G+ LIHP+FWG + IG E     + +  +   W+         DDP++NP  
Sbjct: 184 RLFGVKAVGVVLIHPYFWGKEPIGNEVHELERVLKGIAATWHLACPTTSGCDDPLINPTT 243

Query: 242 EPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 301
           +P L  L   KV + VAEKD L++R   Y E L+K GWGG V+ +E +GEGHVFHLFNPT
Sbjct: 244 DPKLASLGCSKVLVAVAEKDLLRDRDLLYCEALKKCGWGGAVETMEAEGEGHVFHLFNPT 303

Query: 302 CDSAADLVGQLAAFISG 319
           C +A  ++ + AAFISG
Sbjct: 304 CGNAVAMLKKTAAFISG 316

[Close]

BLAST of CmaCh02G004450.1 vs. TAIR10
Match: AT3G48690.1 (AT3G48690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 316.2 bits (809), Expect = 2.3e-86
Identity = 160/321 (49.84%), Postives = 209/321 (65.11%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA++ +P   IYK  RI+RL+G    PP  +P+ GV SKDVV S +++++VRIY P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
           A   + D KLPLLVYFHGGGF IETAFSP YH  L   V+ +N VAVSV+YRRAPEH + 
Sbjct: 64  AA--ETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE--- 184
           +PF+DSWTALKW+  H  G+G ++WLN+ AD ++V+L+GDSAG NI H MA+R A E   
Sbjct: 124 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 183

Query: 185 -GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVN- 244
            GL    I G+ L+HP+FW    I E+   D      +E  W   S + KD  DDP++N 
Sbjct: 184 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 243

Query: 245 -PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHL 304
                 DL  L   KV + VAEKD L  +G  YA  L KSGW G V+VVE++GE HVFHL
Sbjct: 244 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 303

Query: 305 FNPTCDSAADLVGQLAAFISG 319
             P CD+A +++ + + FI G
Sbjct: 304 LKPECDNAIEVMHKFSGFIKG 322

[Close]

BLAST of CmaCh02G004450.1 vs. TAIR10
Match: AT3G48700.1 (AT3G48700.1 carboxyesterase 13)

HSP 1 Score: 297.4 bits (760), Expect = 1.1e-80
Identity = 154/324 (47.53%), Postives = 207/324 (63.89%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP-KS 64
           EIA + +P  IIYK  RI+RL+G    PP  +P+ GV SKDVV S ++++++RIY P K+
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQL 124
           A    +   KLPLLVYFHGGGF +ETAFSP YH  L   V+ ++ VAVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMAL-----RM 184
           P  ++DSWTALKW+ +H  G+G ++WLN+ AD ++V+LAGDSAG NI H M +     ++
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 ATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIV 244
           + E L    I G+ L+HP+FW    + +++  D      +E++W   S + KD  DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTV-DVVETDGEGHV 304
           N       DL  L   KV + VAEKD L  +G  Y E L KS W G V DVVET GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPTCDSAADLVGQLAAFISG 319
           FHL +P  + A +LV + A FI G
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIKG 327

[Close]

BLAST of CmaCh02G004450.1 vs. TAIR10
Match: AT1G47480.1 (AT1G47480.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 297.0 bits (759), Expect = 1.4e-80
Identity = 149/315 (47.30%), Postives = 204/315 (64.76%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           +++L   PW +++ D  ++RL G ++ PPGLDP TGV SKD++I  +  ++ RIYRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
               +  QK+PL++YFHGG F I +   P YH  LN  V +AN++AVSVNYR APEH LP
Sbjct: 67  ----QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLP 126

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +EDSWTALK I A +     + W+N+ ADL+ ++L GDSAG NI+H +A R A +   
Sbjct: 127 TAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQ 186

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNP--EHE 244
            +KIKG+ +IHP+FWG + IG E + D     +V+  W +V    K  DDP +NP  +  
Sbjct: 187 TLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 246

Query: 245 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 304
           PDLG L  E+V I VAEKD L ERG+ Y E L KS W G V+++ET  + HVFH+F P C
Sbjct: 247 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 306

Query: 305 DSAADLVGQLAAFIS 318
           D A ++V  LA FI+
Sbjct: 307 DEAMEMVRCLALFIN 310

[Close]

BLAST of CmaCh02G004450.1 vs. TAIR10
Match: AT2G03550.1 (AT2G03550.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 292.4 bits (747), Expect = 3.5e-79
Identity = 149/313 (47.60%), Postives = 195/313 (62.30%), Query Frame = 1


Query: 6   IALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAA 65
           IA + +P F +YK  RI+RL+G    PP L P+ GV SKD++ S E ++++RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  GSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLPI 125
                 +KLP+L+YFHGGGF IETAFSP YH  L   VA AN +A+SVNYRRAPE  +PI
Sbjct: 65  V-----KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPI 124

Query: 126 PFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAG 185
           P+EDSW +LKW+  H  GTGP+ W+N+  D  +V+LAGDSAGGNI+H + +R   E L  
Sbjct: 125 PYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCD 184

Query: 186 VKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVNPEHEPD 245
             I G+ LIHP+FW    I E +  D      VE  W   S + K  +DDP +N      
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 246 LGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTCDS 305
            G L   +V + VA  D    +G  YAE L+KSGW G V+V+ET  EGHVFHL NP  D+
Sbjct: 245 SG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDN 304

Query: 306 AADLVGQLAAFIS 318
           A  +V +L  FI+
Sbjct: 305 ARQVVKKLEEFIN 311

[Close]

BLAST of CmaCh02G004450.1 vs. TAIR10
Match: AT1G49660.1 (AT1G49660.1 carboxyesterase 5)

HSP 1 Score: 281.6 bits (719), Expect = 6.2e-76
Identity = 141/317 (44.48%), Postives = 192/317 (60.57%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA    P+  IYKD R++RLIG D  P  LDP   V SKDV+ S E++++VR++ P  +
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
                G+ KLPLL+Y HGG + IE+ FSP YH +L   V  AN +AVSV YRRAPE  +P
Sbjct: 64  TKLTAGN-KLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +ED W+A++WI AHSNG+GP +W+N+ AD  +V+L GDSAGGNI+H MA++   E   
Sbjct: 124 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 183

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVS--KDIKDLDDPIVNPEHE 244
            +KIKG+ ++HP FWG   + E    D ++   +  +W  ++    +   DDP+ N    
Sbjct: 184 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 243

Query: 245 -PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 304
             D   L  +KV + VA KD    +G  YA  L K  W GTV+VVE +GE HVFHL NP 
Sbjct: 244 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 303

Query: 305 CDSAADLVGQLAAFISG 319
            D A   + +   FI G
Sbjct: 304 SDKALKFLKKFVEFIIG 319

[Close]

BLAST of CmaCh02G004450.1 vs. NCBI nr
Match: gi|659118607|ref|XP_008459207.1| (PREDICTED: probable carboxylesterase 12 [Cucumis melo])

HSP 1 Score: 564.3 bits (1453), Expect = 1.4e-157
Identity = 263/321 (81.93%), Postives = 289/321 (90.03%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALNAAP+FI+YKD RIDRLIGNDIDPPGLDP TGVESKDV IS +  V VR+YR
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPD--VTVRVYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PKSAA   K  +KLPLLVYFHGGGFCIETAFSPFYHQH++ WVAEANI+AVSVNYRRAPE
Sbjct: 61  PKSAAE--KISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+WT++KWIA+HS G G DEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGGKLIGEE +WDP  + VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGR+ AE+V +YVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRMPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           TCD A +LV QLAAFI+  CR
Sbjct: 301 TCDIAGELVKQLAAFINSGCR 317

[Close]

BLAST of CmaCh02G004450.1 vs. NCBI nr
Match: gi|449455336|ref|XP_004145409.1| (PREDICTED: probable carboxylesterase 12 [Cucumis sativus])

HSP 1 Score: 563.9 bits (1452), Expect = 1.8e-157
Identity = 263/321 (81.93%), Postives = 288/321 (89.72%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALN AP+FI+YKD RIDRLIGNDIDPPGLDPKTGVE+KDV IS +  VAVR+YR
Sbjct: 1   MASDDIALNVAPFFILYKDGRIDRLIGNDIDPPGLDPKTGVETKDVDISPD--VAVRVYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PKS     K  +KLPLLVYFHGGGFCIETAFSPFY+QH++ WVAEANI AVSVNYRRAPE
Sbjct: 61  PKSP--DEKQSEKLPLLVYFHGGGFCIETAFSPFYNQHISAWVAEANIAAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+WTA+KWIA+HS G GPDEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTAMKWIASHSEGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGG+L+GEE +WDPK + VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGELLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGRL AE+V IYVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRLPAERVGIYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           TCD A +LV QLAAFI   CR
Sbjct: 301 TCDMAGELVKQLAAFIKSGCR 317

[Close]

BLAST of CmaCh02G004450.1 vs. NCBI nr
Match: gi|659118611|ref|XP_008459209.1| (PREDICTED: probable carboxylesterase 7 [Cucumis melo])

HSP 1 Score: 456.1 bits (1172), Expect = 5.2e-125
Identity = 211/321 (65.73%), Postives = 254/321 (79.13%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MAS EIA N +P  I+YKD R +RL+GN++  P LDP TGVESKD++IS E  V+ RIYR
Sbjct: 72  MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 131

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PK  A       KLPLL+YFHGGGFCIE+AFSP YH HLN  VAEAN++A+SV YRRAPE
Sbjct: 132 PKPTAEP----HKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPE 191

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           + LPI +ED WTALKW+AAHS GTGP+EWLNEIADLN+VY AGDSAG NIA++MA+R+  
Sbjct: 192 YPLPIAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGI 251

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EG+AG+ +KGL LIHP+FWG KLIG+E++  P+    +E LWY     I  LDDP+VNPE
Sbjct: 252 EGVAGLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPE 311

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
            EP LG LAAE+VA+YVAEKD+LK+RG  Y+ECL+KSGWGG V+VVET G+GHVFHLFNP
Sbjct: 312 FEPKLGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNP 371

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           T D A D VG+LAAFI+G  R
Sbjct: 372 TSDDAVDFVGKLAAFINGGSR 388

[Close]

BLAST of CmaCh02G004450.1 vs. NCBI nr
Match: gi|449474681|ref|XP_004154254.1| (PREDICTED: probable carboxylesterase 13 [Cucumis sativus])

HSP 1 Score: 448.0 bits (1151), Expect = 1.4e-122
Identity = 204/318 (64.15%), Postives = 250/318 (78.62%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MAS EIA N +P  I+YKD R +RL+GN++  P LDP T VESKD+VIS E  V+ RIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PK  A       KLPLL+Y HGGGFCIE+AFSP YH HLN  VAEAN++A+SV YRRAPE
Sbjct: 61  PKPTAEP----HKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           H LPI +EDSWTALKW+AAHS GTGP+EWLN+IAD N+VY AGDSAG N+A++MA+R+  
Sbjct: 121 HPLPIAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGM 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EG+AG+ +KGL L+HP+FWG KLIG+E++  P+    +E LWY     I  LDDPIVNPE
Sbjct: 181 EGVAGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
            EP+LG++ AE+VA+YVAEKD LK+RGR Y+ECL+KSGWGG V+V ET G+GHVFHLFNP
Sbjct: 241 FEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISG 319
           T D A   VG+LAAF++G
Sbjct: 301 TSDDAVQFVGKLAAFLNG 314

[Close]

BLAST of CmaCh02G004450.1 vs. NCBI nr
Match: gi|593787011|ref|XP_007156546.1| (hypothetical protein PHAVU_003G295200g [Phaseolus vulgaris])

HSP 1 Score: 379.0 bits (972), Expect = 8.1e-102
Identity = 175/315 (55.56%), Postives = 231/315 (73.33%), Query Frame = 1


Query: 3   SDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPK 62
           S E+A++ +P+  IYKD  I+R+ GNDI PP LDP T VESKDVVIS++D V+ RI+ PK
Sbjct: 5   STEVAMDLSPFVKIYKDGHIERVTGNDIVPPCLDPATNVESKDVVISKDDDVSARIFIPK 64

Query: 63  SAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQ 122
               +    QKLPLLVYFHGGGFCIET +SP YH+ +N  V++ANIVAVSV+YRRAPEH 
Sbjct: 65  LTDQT----QKLPLLVYFHGGGFCIETPYSPPYHKFINSLVSKANIVAVSVHYRRAPEHP 124

Query: 123 LPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEG 182
           +PI  EDSWT+LKW+ +H +G GP+EWLN  AD  +V+  GDSAG NI+H MALR+   G
Sbjct: 125 VPIAHEDSWTSLKWVVSHFDGNGPEEWLNRHADFGKVFCGGDSAGANISHHMALRIGIHG 184

Query: 183 LAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEHE 242
           L G+ ++G+ L+HP+FWG + IG E +   + + +VENLW + S      DDP++NP  +
Sbjct: 185 LPGMNLQGIVLVHPYFWGVERIGSEAQ---EHVPIVENLWRFTSPASTGSDDPLLNPAKD 244

Query: 243 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 302
           PDLG+L  ++V + VAEKD LK+RG HY E L KS W G  +V+E +GEGHVFHLFNP C
Sbjct: 245 PDLGKLGCKRVLVCVAEKDFLKDRGWHYKELLEKSEWEGVAEVLEANGEGHVFHLFNPDC 304

Query: 303 DSAADLVGQLAAFIS 318
           D+A  L+ Q+A+FI+
Sbjct: 305 DNAVSLLDQIASFIN 312

The following BLAST results are available for this feature:

BLAST of CmaCh02G004450.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CXE12_ARATH	4.0e-85	49.84	Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1	[more]
CXE13_ARATH	1.9e-79	47.53	Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1	[more]
CXE2_ARATH	2.5e-79	47.30	Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1	[more]
CXE7_ARATH	6.2e-78	47.60	Probable carboxylesterase 7 OS=Arabidopsis thaliana GN=CXE7 PE=2 SV=1	[more]
CXE5_ARATH	1.1e-74	44.48	Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1	[more]

BLAST of CmaCh02G004450.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKY5_CUCSA	1.2e-157	81.93	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234510 PE=4 SV=1	[more]
A0A0A0LIH8_CUCSA	9.9e-123	64.15	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234520 PE=4 SV=1	[more]
V7CGY0_PHAVU	5.7e-102	55.56	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G295200g PE=4 SV=1	[more]
G7JMF3_MEDTR	2.6e-99	52.22	CXE carboxylesterase OS=Medicago truncatula GN=MTR_4g086510 PE=4 SV=1	[more]
B9N4W8_POPTR	2.2e-98	53.00	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s14970g PE=4 SV=1	[more]

BLAST of CmaCh02G004450.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G48690.1	2.3e-86	49.84	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48700.1	1.1e-80	47.53	carboxyesterase 13	[more]
AT1G47480.1	1.4e-80	47.30	alpha/beta-Hydrolases superfamily protein	[more]
AT2G03550.1	3.5e-79	47.60	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	6.2e-76	44.48	carboxyesterase 5	[more]

BLAST of CmaCh02G004450.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659118607\|ref\|XP_008459207.1\|	1.4e-157	81.93	PREDICTED: probable carboxylesterase 12 [Cucumis melo]	[more]
gi\|449455336\|ref\|XP_004145409.1\|	1.8e-157	81.93	PREDICTED: probable carboxylesterase 12 [Cucumis sativus]	[more]
gi\|659118611\|ref\|XP_008459209.1\|	5.2e-125	65.73	PREDICTED: probable carboxylesterase 7 [Cucumis melo]	[more]
gi\|449474681\|ref\|XP_004154254.1\|	1.4e-122	64.15	PREDICTED: probable carboxylesterase 13 [Cucumis sativus]	[more]
gi\|593787011\|ref\|XP_007156546.1\|	8.1e-102	55.56	hypothetical protein PHAVU_003G295200g [Phaseolus vulgaris]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh02G004450	CmaCh02G004450	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh02G004450.1	CmaCh02G004450.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh02G004450.1.exon.1	CmaCh02G004450.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh02G004450.1.CDS.1	CmaCh02G004450.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 77..296 score: 2.8
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 3..316 score: 1.4E
None	No IPR available	PANTHER	PTHR23024:SF157	CARBOXYLESTERASE 12-RELATED	coord: 3..316 score: 1.4E