CmaCh02G004450 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh02G004450
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Carboxylesterase 1
Location	Cma_Chr02 : 2275085 .. 2276053 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGTGTGGAGTCTAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCGGGCTCTGGGAAAGGAGATCAGAAGCTTCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTCTACCACCAGCACCTCAACCCTTGGGTGGCTGAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGGCGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGACTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCACATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATACATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAACCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGACAATCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTCGATGTGGTGGAGACCGACGGCGAAGGGCACGTCTTCCATTTGTTCAATCCAACCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCCGTTGTTGA

mRNA sequence

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGTGTGGAGTCTAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCGGGCTCTGGGAAAGGAGATCAGAAGCTTCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTCTACCACCAGCACCTCAACCCTTGGGTGGCTGAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGGCGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGACTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCACATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATACATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAACCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGACAATCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTCGATGTGGTGGAGACCGACGGCGAAGGGCACGTCTTCCATTTGTTCAATCCAACCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCCGTTGTTGA

Coding sequence (CDS)

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGTGTGGAGTCTAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCGGGCTCTGGGAAAGGAGATCAGAAGCTTCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTCTACCACCAGCACCTCAACCCTTGGGTGGCTGAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGGCGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGACTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCACATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATACATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAACCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGACAATCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTCGATGTGGTGGAGACCGACGGCGAAGGGCACGTCTTCCATTTGTTCAATCCAACCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCCGTTGTTGA

Protein sequence

MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTCDSAADLVGQLAAFISGKCRC

Homology

[Close]

BLAST of CmaCh02G004450 vs. Swiss-Prot
Match: CXE12_ARATH (Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.0e-85
Identity = 160/321 (49.84%), Postives = 209/321 (65.11%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA++ +P   IYK  RI+RL+G    PP  +P+ GV SKDVV S +++++VRIY P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
           A   + D KLPLLVYFHGGGF IETAFSP YH  L   V+ +N VAVSV+YRRAPEH + 
Sbjct: 64  AA--ETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE--- 184
           +PF+DSWTALKW+  H  G+G ++WLN+ AD ++V+L+GDSAG NI H MA+R A E   
Sbjct: 124 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 183

Query: 185 -GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVN- 244
            GL    I G+ L+HP+FW    I E+   D      +E  W   S + KD  DDP++N 
Sbjct: 184 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 243

Query: 245 -PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHL 304
                 DL  L   KV + VAEKD L  +G  YA  L KSGW G V+VVE++GE HVFHL
Sbjct: 244 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 303

Query: 305 FNPTCDSAADLVGQLAAFISG 319
             P CD+A +++ + + FI G
Sbjct: 304 LKPECDNAIEVMHKFSGFIKG 322

[Close]

BLAST of CmaCh02G004450 vs. Swiss-Prot
Match: CXE13_ARATH (Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.9e-79
Identity = 154/324 (47.53%), Postives = 207/324 (63.89%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP-KS 64
           EIA + +P  IIYK  RI+RL+G    PP  +P+ GV SKDVV S ++++++RIY P K+
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQL 124
           A    +   KLPLLVYFHGGGF +ETAFSP YH  L   V+ ++ VAVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMAL-----RM 184
           P  ++DSWTALKW+ +H  G+G ++WLN+ AD ++V+LAGDSAG NI H M +     ++
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 ATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIV 244
           + E L    I G+ L+HP+FW    + +++  D      +E++W   S + KD  DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTV-DVVETDGEGHV 304
           N       DL  L   KV + VAEKD L  +G  Y E L KS W G V DVVET GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPTCDSAADLVGQLAAFISG 319
           FHL +P  + A +LV + A FI G
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIKG 327

[Close]

BLAST of CmaCh02G004450 vs. Swiss-Prot
Match: CXE2_ARATH (Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.5e-79
Identity = 149/315 (47.30%), Postives = 204/315 (64.76%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           +++L   PW +++ D  ++RL G ++ PPGLDP TGV SKD++I  +  ++ RIYRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
               +  QK+PL++YFHGG F I +   P YH  LN  V +AN++AVSVNYR APEH LP
Sbjct: 67  ----QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLP 126

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +EDSWTALK I A +     + W+N+ ADL+ ++L GDSAG NI+H +A R A +   
Sbjct: 127 TAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQ 186

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNP--EHE 244
            +KIKG+ +IHP+FWG + IG E + D     +V+  W +V    K  DDP +NP  +  
Sbjct: 187 TLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 246

Query: 245 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 304
           PDLG L  E+V I VAEKD L ERG+ Y E L KS W G V+++ET  + HVFH+F P C
Sbjct: 247 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 306

Query: 305 DSAADLVGQLAAFIS 318
           D A ++V  LA FI+
Sbjct: 307 DEAMEMVRCLALFIN 310

[Close]

BLAST of CmaCh02G004450 vs. Swiss-Prot
Match: CXE7_ARATH (Probable carboxylesterase 7 OS=Arabidopsis thaliana GN=CXE7 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.2e-78
Identity = 149/313 (47.60%), Postives = 195/313 (62.30%), Query Frame = 1


Query: 6   IALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAA 65
           IA + +P F +YK  RI+RL+G    PP L P+ GV SKD++ S E ++++RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  GSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLPI 125
                 +KLP+L+YFHGGGF IETAFSP YH  L   VA AN +A+SVNYRRAPE  +PI
Sbjct: 65  V-----KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPI 124

Query: 126 PFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAG 185
           P+EDSW +LKW+  H  GTGP+ W+N+  D  +V+LAGDSAGGNI+H + +R   E L  
Sbjct: 125 PYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCD 184

Query: 186 VKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVNPEHEPD 245
             I G+ LIHP+FW    I E +  D      VE  W   S + K  +DDP +N      
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 246 LGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTCDS 305
            G L   +V + VA  D    +G  YAE L+KSGW G V+V+ET  EGHVFHL NP  D+
Sbjct: 245 SG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDN 304

Query: 306 AADLVGQLAAFIS 318
           A  +V +L  FI+
Sbjct: 305 ARQVVKKLEEFIN 311

[Close]

BLAST of CmaCh02G004450 vs. Swiss-Prot
Match: CXE5_ARATH (Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-74
Identity = 141/317 (44.48%), Postives = 192/317 (60.57%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA    P+  IYKD R++RLIG D  P  LDP   V SKDV+ S E++++VR++ P  +
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
                G+ KLPLL+Y HGG + IE+ FSP YH +L   V  AN +AVSV YRRAPE  +P
Sbjct: 64  TKLTAGN-KLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +ED W+A++WI AHSNG+GP +W+N+ AD  +V+L GDSAGGNI+H MA++   E   
Sbjct: 124 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 183

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVS--KDIKDLDDPIVNPEHE 244
            +KIKG+ ++HP FWG   + E    D ++   +  +W  ++    +   DDP+ N    
Sbjct: 184 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 243

Query: 245 -PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 304
             D   L  +KV + VA KD    +G  YA  L K  W GTV+VVE +GE HVFHL NP 
Sbjct: 244 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 303

Query: 305 CDSAADLVGQLAAFISG 319
            D A   + +   FI G
Sbjct: 304 SDKALKFLKKFVEFIIG 319

[Close]

BLAST of CmaCh02G004450 vs. TrEMBL
Match: A0A0A0LKY5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234510 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.2e-157
Identity = 263/321 (81.93%), Postives = 288/321 (89.72%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALN AP+FI+YKD RIDRLIGNDIDPPGLDPKTGVE+KDV IS +  VAVR+YR
Sbjct: 1   MASDDIALNVAPFFILYKDGRIDRLIGNDIDPPGLDPKTGVETKDVDISPD--VAVRVYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PKS     K  +KLPLLVYFHGGGFCIETAFSPFY+QH++ WVAEANI AVSVNYRRAPE
Sbjct: 61  PKSP--DEKQSEKLPLLVYFHGGGFCIETAFSPFYNQHISAWVAEANIAAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+WTA+KWIA+HS G GPDEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTAMKWIASHSEGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGG+L+GEE +WDPK + VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGELLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGRL AE+V IYVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRLPAERVGIYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           TCD A +LV QLAAFI   CR
Sbjct: 301 TCDMAGELVKQLAAFIKSGCR 317

[Close]

BLAST of CmaCh02G004450 vs. TrEMBL
Match: A0A0A0LIH8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234520 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 9.9e-123
Identity = 204/318 (64.15%), Postives = 250/318 (78.62%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MAS EIA N +P  I+YKD R +RL+GN++  P LDP T VESKD+VIS E  V+ RIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PK  A       KLPLL+Y HGGGFCIE+AFSP YH HLN  VAEAN++A+SV YRRAPE
Sbjct: 61  PKPTAEP----HKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           H LPI +EDSWTALKW+AAHS GTGP+EWLN+IAD N+VY AGDSAG N+A++MA+R+  
Sbjct: 121 HPLPIAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGM 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EG+AG+ +KGL L+HP+FWG KLIG+E++  P+    +E LWY     I  LDDPIVNPE
Sbjct: 181 EGVAGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
            EP+LG++ AE+VA+YVAEKD LK+RGR Y+ECL+KSGWGG V+V ET G+GHVFHLFNP
Sbjct: 241 FEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISG 319
           T D A   VG+LAAF++G
Sbjct: 301 TSDDAVQFVGKLAAFLNG 314

[Close]

BLAST of CmaCh02G004450 vs. TrEMBL
Match: V7CGY0_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G295200g PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.7e-102
Identity = 175/315 (55.56%), Postives = 231/315 (73.33%), Query Frame = 1


Query: 3   SDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPK 62
           S E+A++ +P+  IYKD  I+R+ GNDI PP LDP T VESKDVVIS++D V+ RI+ PK
Sbjct: 5   STEVAMDLSPFVKIYKDGHIERVTGNDIVPPCLDPATNVESKDVVISKDDDVSARIFIPK 64

Query: 63  SAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQ 122
               +    QKLPLLVYFHGGGFCIET +SP YH+ +N  V++ANIVAVSV+YRRAPEH 
Sbjct: 65  LTDQT----QKLPLLVYFHGGGFCIETPYSPPYHKFINSLVSKANIVAVSVHYRRAPEHP 124

Query: 123 LPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEG 182
           +PI  EDSWT+LKW+ +H +G GP+EWLN  AD  +V+  GDSAG NI+H MALR+   G
Sbjct: 125 VPIAHEDSWTSLKWVVSHFDGNGPEEWLNRHADFGKVFCGGDSAGANISHHMALRIGIHG 184

Query: 183 LAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEHE 242
           L G+ ++G+ L+HP+FWG + IG E +   + + +VENLW + S      DDP++NP  +
Sbjct: 185 LPGMNLQGIVLVHPYFWGVERIGSEAQ---EHVPIVENLWRFTSPASTGSDDPLLNPAKD 244

Query: 243 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 302
           PDLG+L  ++V + VAEKD LK+RG HY E L KS W G  +V+E +GEGHVFHLFNP C
Sbjct: 245 PDLGKLGCKRVLVCVAEKDFLKDRGWHYKELLEKSEWEGVAEVLEANGEGHVFHLFNPDC 304

Query: 303 DSAADLVGQLAAFIS 318
           D+A  L+ Q+A+FI+
Sbjct: 305 DNAVSLLDQIASFIN 312

[Close]

BLAST of CmaCh02G004450 vs. TrEMBL
Match: G7JMF3_MEDTR (CXE carboxylesterase OS=Medicago truncatula GN=MTR_4g086510 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.6e-99
Identity = 165/316 (52.22%), Postives = 229/316 (72.47%), Query Frame = 1


Query: 2   ASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP 61
           +S+E+ L+ +P   IYKD  I+RLIG+DI PP  DP T VESKD++IS++ +++ RI+ P
Sbjct: 4   SSNEVVLDLSPMIKIYKDGHIERLIGSDIVPPSFDPTTNVESKDILISKDQNISARIFIP 63

Query: 62  KSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEH 121
           K        +QKLPLLVYFHGGGFC+ET FSP YH  LN  V++AN++AVSV+YRRAPEH
Sbjct: 64  K-LNNDQFPNQKLPLLVYFHGGGFCVETPFSPPYHNFLNTIVSKANVIAVSVDYRRAPEH 123

Query: 122 QLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE 181
            LPI +EDSWT+LKW+ +H +G G DEW+N  AD  +++ AGDSAG NIA+ MA+R+ T+
Sbjct: 124 PLPIAYEDSWTSLKWVVSHLHGNGSDEWINRYADFGKMFFAGDSAGANIANHMAIRVGTQ 183

Query: 182 GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEH 241
           GL G+ ++G+ L+H  FWG + +G E     + + + +NLW +V       DDP +NP  
Sbjct: 184 GLQGINLEGIVLVHTFFWGVERVGSEATEKSEHLSLADNLWRFVCPTSSGSDDPFLNPGK 243

Query: 242 EPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 301
           + +LGRL  ++V + VAE D+LK+RG +Y E L K G+GG V+V+ET GEGHVFHLFNP 
Sbjct: 244 DKNLGRLGCKRVLVCVAENDSLKDRGWYYKELLEKIGYGGVVEVIETKGEGHVFHLFNPN 303

Query: 302 CDSAADLVGQLAAFIS 318
           CD+A  L+ Q+A+FI+
Sbjct: 304 CDNAISLLNQIASFIN 318

[Close]

BLAST of CmaCh02G004450 vs. TrEMBL
Match: B9N4W8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s14970g PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.2e-98
Identity = 168/317 (53.00%), Postives = 221/317 (69.72%), Query Frame = 1


Query: 2   ASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP 61
           A  + AL+ +P+ I+YKD  I+RL+GN+I PP LDPK+ V SKD V S+E  ++ R+Y P
Sbjct: 4   AEPDAALDLSPFIIVYKDGSIERLVGNEIVPPSLDPKSSVLSKDAVYSKEAKLSSRLYLP 63

Query: 62  KSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEH 121
                    D+KLPLL+YF+GGGFC+E+AFSP YH +LN  VAEA ++AVSV+YRR PEH
Sbjct: 64  PGV----DPDKKLPLLIYFYGGGFCVESAFSPAYHNYLNILVAEAKVIAVSVDYRRVPEH 123

Query: 122 QLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE 181
            +P+P++DSWTALKW+A+H NG GP++WLN  AD  +VYLAGDSAGGNIAH MA+R   E
Sbjct: 124 PIPVPYDDSWTALKWVASHVNGDGPEKWLNNHADFGKVYLAGDSAGGNIAHHMAMRYGQE 183

Query: 182 GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEH 241
            L GVK  G+ LIHP+FWG + IG E     + +  +   W+         DDP++NP  
Sbjct: 184 RLFGVKAVGVVLIHPYFWGKEPIGNEVHELERVLKGIAATWHLACPTTSGCDDPLINPTT 243

Query: 242 EPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 301
           +P L  L   KV + VAEKD L++R   Y E L+K GWGG V+ +E +GEGHVFHLFNPT
Sbjct: 244 DPKLASLGCSKVLVAVAEKDLLRDRDLLYCEALKKCGWGGAVETMEAEGEGHVFHLFNPT 303

Query: 302 CDSAADLVGQLAAFISG 319
           C +A  ++ + AAFISG
Sbjct: 304 CGNAVAMLKKTAAFISG 316

[Close]

BLAST of CmaCh02G004450 vs. TAIR10
Match: AT3G48690.1 (AT3G48690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 316.2 bits (809), Expect = 2.3e-86
Identity = 160/321 (49.84%), Postives = 209/321 (65.11%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA++ +P   IYK  RI+RL+G    PP  +P+ GV SKDVV S +++++VRIY P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
           A   + D KLPLLVYFHGGGF IETAFSP YH  L   V+ +N VAVSV+YRRAPEH + 
Sbjct: 64  AA--ETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE--- 184
           +PF+DSWTALKW+  H  G+G ++WLN+ AD ++V+L+GDSAG NI H MA+R A E   
Sbjct: 124 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 183

Query: 185 -GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVN- 244
            GL    I G+ L+HP+FW    I E+   D      +E  W   S + KD  DDP++N 
Sbjct: 184 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 243

Query: 245 -PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHL 304
                 DL  L   KV + VAEKD L  +G  YA  L KSGW G V+VVE++GE HVFHL
Sbjct: 244 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 303

Query: 305 FNPTCDSAADLVGQLAAFISG 319
             P CD+A +++ + + FI G
Sbjct: 304 LKPECDNAIEVMHKFSGFIKG 322

[Close]

BLAST of CmaCh02G004450 vs. TAIR10
Match: AT3G48700.1 (AT3G48700.1 carboxyesterase 13)

HSP 1 Score: 297.4 bits (760), Expect = 1.1e-80
Identity = 154/324 (47.53%), Postives = 207/324 (63.89%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRP-KS 64
           EIA + +P  IIYK  RI+RL+G    PP  +P+ GV SKDVV S ++++++RIY P K+
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQL 124
           A    +   KLPLLVYFHGGGF +ETAFSP YH  L   V+ ++ VAVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMAL-----RM 184
           P  ++DSWTALKW+ +H  G+G ++WLN+ AD ++V+LAGDSAG NI H M +     ++
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 ATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIV 244
           + E L    I G+ L+HP+FW    + +++  D      +E++W   S + KD  DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTV-DVVETDGEGHV 304
           N       DL  L   KV + VAEKD L  +G  Y E L KS W G V DVVET GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPTCDSAADLVGQLAAFISG 319
           FHL +P  + A +LV + A FI G
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIKG 327

[Close]

BLAST of CmaCh02G004450 vs. TAIR10
Match: AT1G47480.1 (AT1G47480.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 297.0 bits (759), Expect = 1.4e-80
Identity = 149/315 (47.30%), Postives = 204/315 (64.76%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           +++L   PW +++ D  ++RL G ++ PPGLDP TGV SKD++I  +  ++ RIYRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
               +  QK+PL++YFHGG F I +   P YH  LN  V +AN++AVSVNYR APEH LP
Sbjct: 67  ----QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLP 126

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +EDSWTALK I A +     + W+N+ ADL+ ++L GDSAG NI+H +A R A +   
Sbjct: 127 TAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQ 186

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNP--EHE 244
            +KIKG+ +IHP+FWG + IG E + D     +V+  W +V    K  DDP +NP  +  
Sbjct: 187 TLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 246

Query: 245 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 304
           PDLG L  E+V I VAEKD L ERG+ Y E L KS W G V+++ET  + HVFH+F P C
Sbjct: 247 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 306

Query: 305 DSAADLVGQLAAFIS 318
           D A ++V  LA FI+
Sbjct: 307 DEAMEMVRCLALFIN 310

[Close]

BLAST of CmaCh02G004450 vs. TAIR10
Match: AT2G03550.1 (AT2G03550.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 292.4 bits (747), Expect = 3.5e-79
Identity = 149/313 (47.60%), Postives = 195/313 (62.30%), Query Frame = 1


Query: 6   IALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAA 65
           IA + +P F +YK  RI+RL+G    PP L P+ GV SKD++ S E ++++RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  GSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLPI 125
                 +KLP+L+YFHGGGF IETAFSP YH  L   VA AN +A+SVNYRRAPE  +PI
Sbjct: 65  V-----KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPI 124

Query: 126 PFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAG 185
           P+EDSW +LKW+  H  GTGP+ W+N+  D  +V+LAGDSAGGNI+H + +R   E L  
Sbjct: 125 PYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCD 184

Query: 186 VKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKD-LDDPIVNPEHEPD 245
             I G+ LIHP+FW    I E +  D      VE  W   S + K  +DDP +N      
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 246 LGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTCDS 305
            G L   +V + VA  D    +G  YAE L+KSGW G V+V+ET  EGHVFHL NP  D+
Sbjct: 245 SG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDN 304

Query: 306 AADLVGQLAAFIS 318
           A  +V +L  FI+
Sbjct: 305 ARQVVKKLEEFIN 311

[Close]

BLAST of CmaCh02G004450 vs. TAIR10
Match: AT1G49660.1 (AT1G49660.1 carboxyesterase 5)

HSP 1 Score: 281.6 bits (719), Expect = 6.2e-76
Identity = 141/317 (44.48%), Postives = 192/317 (60.57%), Query Frame = 1


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA    P+  IYKD R++RLIG D  P  LDP   V SKDV+ S E++++VR++ P  +
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQLP 124
                G+ KLPLL+Y HGG + IE+ FSP YH +L   V  AN +AVSV YRRAPE  +P
Sbjct: 64  TKLTAGN-KLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 123

Query: 125 IPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +ED W+A++WI AHSNG+GP +W+N+ AD  +V+L GDSAGGNI+H MA++   E   
Sbjct: 124 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 183

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVS--KDIKDLDDPIVNPEHE 244
            +KIKG+ ++HP FWG   + E    D ++   +  +W  ++    +   DDP+ N    
Sbjct: 184 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 243

Query: 245 -PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPT 304
             D   L  +KV + VA KD    +G  YA  L K  W GTV+VVE +GE HVFHL NP 
Sbjct: 244 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 303

Query: 305 CDSAADLVGQLAAFISG 319
            D A   + +   FI G
Sbjct: 304 SDKALKFLKKFVEFIIG 319

[Close]

BLAST of CmaCh02G004450 vs. NCBI nr
Match: gi|659118607|ref|XP_008459207.1| (PREDICTED: probable carboxylesterase 12 [Cucumis melo])

HSP 1 Score: 564.3 bits (1453), Expect = 1.4e-157
Identity = 263/321 (81.93%), Postives = 289/321 (90.03%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALNAAP+FI+YKD RIDRLIGNDIDPPGLDP TGVESKDV IS +  V VR+YR
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPD--VTVRVYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PKSAA   K  +KLPLLVYFHGGGFCIETAFSPFYHQH++ WVAEANI+AVSVNYRRAPE
Sbjct: 61  PKSAAE--KISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+WT++KWIA+HS G G DEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGGKLIGEE +WDP  + VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGR+ AE+V +YVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRMPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           TCD A +LV QLAAFI+  CR
Sbjct: 301 TCDIAGELVKQLAAFINSGCR 317

[Close]

BLAST of CmaCh02G004450 vs. NCBI nr
Match: gi|449455336|ref|XP_004145409.1| (PREDICTED: probable carboxylesterase 12 [Cucumis sativus])

HSP 1 Score: 563.9 bits (1452), Expect = 1.8e-157
Identity = 263/321 (81.93%), Postives = 288/321 (89.72%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALN AP+FI+YKD RIDRLIGNDIDPPGLDPKTGVE+KDV IS +  VAVR+YR
Sbjct: 1   MASDDIALNVAPFFILYKDGRIDRLIGNDIDPPGLDPKTGVETKDVDISPD--VAVRVYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PKS     K  +KLPLLVYFHGGGFCIETAFSPFY+QH++ WVAEANI AVSVNYRRAPE
Sbjct: 61  PKSP--DEKQSEKLPLLVYFHGGGFCIETAFSPFYNQHISAWVAEANIAAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+WTA+KWIA+HS G GPDEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTAMKWIASHSEGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGG+L+GEE +WDPK + VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGELLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGRL AE+V IYVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRLPAERVGIYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           TCD A +LV QLAAFI   CR
Sbjct: 301 TCDMAGELVKQLAAFIKSGCR 317

[Close]

BLAST of CmaCh02G004450 vs. NCBI nr
Match: gi|659118611|ref|XP_008459209.1| (PREDICTED: probable carboxylesterase 7 [Cucumis melo])

HSP 1 Score: 456.1 bits (1172), Expect = 5.2e-125
Identity = 211/321 (65.73%), Postives = 254/321 (79.13%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MAS EIA N +P  I+YKD R +RL+GN++  P LDP TGVESKD++IS E  V+ RIYR
Sbjct: 72  MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 131

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PK  A       KLPLL+YFHGGGFCIE+AFSP YH HLN  VAEAN++A+SV YRRAPE
Sbjct: 132 PKPTAEP----HKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPE 191

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           + LPI +ED WTALKW+AAHS GTGP+EWLNEIADLN+VY AGDSAG NIA++MA+R+  
Sbjct: 192 YPLPIAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGI 251

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EG+AG+ +KGL LIHP+FWG KLIG+E++  P+    +E LWY     I  LDDP+VNPE
Sbjct: 252 EGVAGLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPE 311

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
            EP LG LAAE+VA+YVAEKD+LK+RG  Y+ECL+KSGWGG V+VVET G+GHVFHLFNP
Sbjct: 312 FEPKLGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNP 371

Query: 301 TCDSAADLVGQLAAFISGKCR 322
           T D A D VG+LAAFI+G  R
Sbjct: 372 TSDDAVDFVGKLAAFINGGSR 388

[Close]

BLAST of CmaCh02G004450 vs. NCBI nr
Match: gi|449474681|ref|XP_004154254.1| (PREDICTED: probable carboxylesterase 13 [Cucumis sativus])

HSP 1 Score: 448.0 bits (1151), Expect = 1.4e-122
Identity = 204/318 (64.15%), Postives = 250/318 (78.62%), Query Frame = 1


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MAS EIA N +P  I+YKD R +RL+GN++  P LDP T VESKD+VIS E  V+ RIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120
           PK  A       KLPLL+Y HGGGFCIE+AFSP YH HLN  VAEAN++A+SV YRRAPE
Sbjct: 61  PKPTAEP----HKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPE 120

Query: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           H LPI +EDSWTALKW+AAHS GTGP+EWLN+IAD N+VY AGDSAG N+A++MA+R+  
Sbjct: 121 HPLPIAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGM 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240
           EG+AG+ +KGL L+HP+FWG KLIG+E++  P+    +E LWY     I  LDDPIVNPE
Sbjct: 181 EGVAGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
            EP+LG++ AE+VA+YVAEKD LK+RGR Y+ECL+KSGWGG V+V ET G+GHVFHLFNP
Sbjct: 241 FEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNP 300

Query: 301 TCDSAADLVGQLAAFISG 319
           T D A   VG+LAAF++G
Sbjct: 301 TSDDAVQFVGKLAAFLNG 314

[Close]

BLAST of CmaCh02G004450 vs. NCBI nr
Match: gi|593787011|ref|XP_007156546.1| (hypothetical protein PHAVU_003G295200g [Phaseolus vulgaris])

HSP 1 Score: 379.0 bits (972), Expect = 8.1e-102
Identity = 175/315 (55.56%), Postives = 231/315 (73.33%), Query Frame = 1


Query: 3   SDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPK 62
           S E+A++ +P+  IYKD  I+R+ GNDI PP LDP T VESKDVVIS++D V+ RI+ PK
Sbjct: 5   STEVAMDLSPFVKIYKDGHIERVTGNDIVPPCLDPATNVESKDVVISKDDDVSARIFIPK 64

Query: 63  SAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPEHQ 122
               +    QKLPLLVYFHGGGFCIET +SP YH+ +N  V++ANIVAVSV+YRRAPEH 
Sbjct: 65  LTDQT----QKLPLLVYFHGGGFCIETPYSPPYHKFINSLVSKANIVAVSVHYRRAPEHP 124

Query: 123 LPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEG 182
           +PI  EDSWT+LKW+ +H +G GP+EWLN  AD  +V+  GDSAG NI+H MALR+   G
Sbjct: 125 VPIAHEDSWTSLKWVVSHFDGNGPEEWLNRHADFGKVFCGGDSAGANISHHMALRIGIHG 184

Query: 183 LAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPEHE 242
           L G+ ++G+ L+HP+FWG + IG E +   + + +VENLW + S      DDP++NP  +
Sbjct: 185 LPGMNLQGIVLVHPYFWGVERIGSEAQ---EHVPIVENLWRFTSPASTGSDDPLLNPAKD 244

Query: 243 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPTC 302
           PDLG+L  ++V + VAEKD LK+RG HY E L KS W G  +V+E +GEGHVFHLFNP C
Sbjct: 245 PDLGKLGCKRVLVCVAEKDFLKDRGWHYKELLEKSEWEGVAEVLEANGEGHVFHLFNPDC 304

Query: 303 DSAADLVGQLAAFIS 318
           D+A  L+ Q+A+FI+
Sbjct: 305 DNAVSLLDQIASFIN 312

The following BLAST results are available for this feature:

BLAST of CmaCh02G004450 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CXE12_ARATH	4.0e-85	49.84	Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1	[more]
CXE13_ARATH	1.9e-79	47.53	Probable carboxylesterase 13 OS=Arabidopsis thaliana GN=CXE13 PE=1 SV=1	[more]
CXE2_ARATH	2.5e-79	47.30	Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1	[more]
CXE7_ARATH	6.2e-78	47.60	Probable carboxylesterase 7 OS=Arabidopsis thaliana GN=CXE7 PE=2 SV=1	[more]
CXE5_ARATH	1.1e-74	44.48	Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1	[more]

BLAST of CmaCh02G004450 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKY5_CUCSA	1.2e-157	81.93	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234510 PE=4 SV=1	[more]
A0A0A0LIH8_CUCSA	9.9e-123	64.15	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234520 PE=4 SV=1	[more]
V7CGY0_PHAVU	5.7e-102	55.56	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_003G295200g PE=4 SV=1	[more]
G7JMF3_MEDTR	2.6e-99	52.22	CXE carboxylesterase OS=Medicago truncatula GN=MTR_4g086510 PE=4 SV=1	[more]
B9N4W8_POPTR	2.2e-98	53.00	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s14970g PE=4 SV=1	[more]

BLAST of CmaCh02G004450 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G48690.1	2.3e-86	49.84	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48700.1	1.1e-80	47.53	carboxyesterase 13	[more]
AT1G47480.1	1.4e-80	47.30	alpha/beta-Hydrolases superfamily protein	[more]
AT2G03550.1	3.5e-79	47.60	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	6.2e-76	44.48	carboxyesterase 5	[more]

BLAST of CmaCh02G004450 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659118607\|ref\|XP_008459207.1\|	1.4e-157	81.93	PREDICTED: probable carboxylesterase 12 [Cucumis melo]	[more]
gi\|449455336\|ref\|XP_004145409.1\|	1.8e-157	81.93	PREDICTED: probable carboxylesterase 12 [Cucumis sativus]	[more]
gi\|659118611\|ref\|XP_008459209.1\|	5.2e-125	65.73	PREDICTED: probable carboxylesterase 7 [Cucumis melo]	[more]
gi\|449474681\|ref\|XP_004154254.1\|	1.4e-122	64.15	PREDICTED: probable carboxylesterase 13 [Cucumis sativus]	[more]
gi\|593787011\|ref\|XP_007156546.1\|	8.1e-102	55.56	hypothetical protein PHAVU_003G295200g [Phaseolus vulgaris]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G004450.1	CmaCh02G004450.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 77..296 score: 2.8
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 3..316 score: 1.4E
None	No IPR available	PANTHER	PTHR23024:SF157	CARBOXYLESTERASE 12-RELATED	coord: 3..316 score: 1.4E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh02G004450	Cucsa.085210	Cucumber (Gy14) v1	cgycmaB0161
CmaCh02G004450	CmoCh20G009010	Cucurbita moschata (Rifu)	cmacmoB606
CmaCh02G004450	CmoCh02G004540	Cucurbita moschata (Rifu)	cmacmoB619
CmaCh02G004450	Lsi10G002900	Bottle gourd (USVL1VR-Ls)	cmalsiB548
CmaCh02G004450	Cp4.1LG16g02130	Cucurbita pepo (Zucchini)	cmacpeB626
CmaCh02G004450	Cla97C09G178820	Watermelon (97103) v2	cmawmbB663
CmaCh02G004450	Bhi10G001754	Wax gourd	cmawgoB0762

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh02G004450	CmaCh20G008960	Cucurbita maxima (Rimu)	cmacmaB469

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh02G004450	Cucumber (Chinese Long) v3	cmacucB0701
CmaCh02G004450	Cucurbita maxima (Rimu)	cmacmaB510
CmaCh02G004450	Cucumber (Gy14) v1	cgycmaB0254
CmaCh02G004450	Wild cucumber (PI 183967)	cmacpiB593
CmaCh02G004450	Cucumber (Chinese Long) v2	cmacuB588
CmaCh02G004450	Melon (DHL92) v3.5.1	cmameB534
CmaCh02G004450	Watermelon (Charleston Gray)	cmawcgB510
CmaCh02G004450	Watermelon (97103) v1	cmawmB548
CmaCh02G004450	Cucurbita pepo (Zucchini)	cmacpeB646
CmaCh02G004450	Cucumber (Gy14) v2	cgybcmaB229
CmaCh02G004450	Melon (DHL92) v3.6.1	cmamedB614
CmaCh02G004450	Silver-seed gourd	carcmaB1105
CmaCh02G004450	Silver-seed gourd	carcmaB1460