CmaCh20G008960 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh20G008960
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Carboxylesterase 1
Location	Cma_Chr20 : 4314016 .. 4314527 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCGCGGCCCATTCAGGGGGAGCGGGGCCGGATGAGTGGCTGAATGAAATTGCGGATTTGAACCGGGTTTATTTGGGTGGAGATAGCGCCGGAGGAAACATTGCACATAGGATGGCGCTGCGGGTGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCGCATTTTTTTGGGGGAAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCAAAGGATATATTTGTGGTGAAGAATCTGTGGTTTGTGGTTTCGAAGGATATTAAGGAGCTGGATCACCCGATTGTGAACCCGGAATACGACCCGGATTTGGGGTGGCTTCCGGCGGAGAAAGTGGCGGTTTATGTGGCGGAGAAGGACAATCTAAAGGAGAGGGGACTACAGTACGCGGAATGTCTGAGAAATTCAGGTTGGGACGGGGTCGTTGAGGTGGTCGAAACCGACGATGAAGGCCATGTCTTCCATTTGTTCAATCCGACCAGCGACTCAGCG

mRNA sequence

ATGGGGGGAGCGGGGCCGGATGAGTGGCTGAATGAAATTGCGGATTTGAACCGGGTTTATTTGGGTGGAGATAGCGCCGGAGGAAACATTGCACATAGGATGGCGCTGCGGGTGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCGCATTTTTTTGGGGGAAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCAAAGGATATATTTGTGGTGAAGAATCTGTGGATATTAAGGAGCTGGATCACCCGATTGTGAACCCGGAATACGACCCGGATTTGGGGTGGCTTCCGGCGGAGAAAGTGGCGGTTTATGTGGCGGAGAAGGACAATCTAAAGGAGAGGGGACTACAGTACGCGGAATGTCTGAGAAATTCAGGTTGGGACGGGGTCGTTGAGGTGGTCGAAACCGACGATGAAGGCCATGTCTTCCATTTGTTCAATCCGACCAGCGACTCAGCG

Coding sequence (CDS)

ATGGGGGGAGCGGGGCCGGATGAGTGGCTGAATGAAATTGCGGATTTGAACCGGGTTTATTTGGGTGGAGATAGCGCCGGAGGAAACATTGCACATAGGATGGCGCTGCGGGTGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCGCATTTTTTTGGGGGAAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCAAAGGATATATTTGTGGTGAAGAATCTGTGGATATTAAGGAGCTGGATCACCCGATTGTGAACCCGGAATACGACCCGGATTTGGGGTGGCTTCCGGCGGAGAAAGTGGCGGTTTATGTGGCGGAGAAGGACAATCTAAAGGAGAGGGGACTACAGTACGCGGAATGTCTGAGAAATTCAGGTTGGGACGGGGTCGTTGAGGTGGTCGAAACCGACGATGAAGGCCATGTCTTCCATTTGTTCAATCCGACCAGCGACTCAGCG

Protein sequence

MGGAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGKIDRRRKGVGSKGYICGEESVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA

Homology

[Close]

BLAST of CmaCh20G008960 vs. Swiss-Prot
Match: CXE2_ARATH (Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.8e-28
Identity = 72/164 (43.90%), Postives = 98/164 (59.76%), Query Frame = 1


Query: 7   DEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGKI--- 66
           + W+N+ ADL+ ++L GDSAG NI+H +A R A +    +KIKG+ +IHP+F+G +    
Sbjct: 140 EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQTLKIKGIGMIHPYFWGTQPIGA 199

Query: 67  ---DRRRKGV--GSKGYICGEESVDIKELDHPIVNPEYD--PDLGWLPAEKVAVYVAEKD 126
              D  RK +  G   ++C  E    K  D P +NP  D  PDLG L  E+V + VAEKD
Sbjct: 200 EIKDEARKQMVDGWWEFVCPSE----KGSDDPWINPFADGSPDLGGLGCERVMITVAEKD 259

Query: 127 NLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
            L ERG  Y E L  S W G VE++ET ++ HVFH+F P  D A
Sbjct: 260 ILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEA 298

[Close]

BLAST of CmaCh20G008960 vs. Swiss-Prot
Match: CXE5_ARATH (Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.6e-27
Identity = 69/166 (41.57%), Postives = 92/166 (55.42%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG-- 62
           G+GP +W+N+ AD  +V+LGGDSAGGNI+H MA++   E    +KIKG+ ++HP F+G  
Sbjct: 141 GSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKLDLKIKGIAVVHPAFWGTD 200

Query: 63  --GKIDRRRKGVGSKGYICGEESV---DIKELDHPIVNPE-YDPDLGWLPAEKVAVYVAE 122
              + D + K   S      E+      +   D P+ N      D   L  +KV V VA 
Sbjct: 201 PVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSGSDFSGLGCDKVLVAVAG 260

Query: 123 KDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           KD    +GL YA  L    W+G VEVVE + E HVFHL NP SD A
Sbjct: 261 KDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKSDKA 306

[Close]

BLAST of CmaCh20G008960 vs. Swiss-Prot
Match: CXE7_ARATH (Probable carboxylesterase 7 OS=Arabidopsis thaliana GN=CXE7 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.8e-26
Identity = 69/166 (41.57%), Postives = 92/166 (55.42%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG-G 62
           G GP+ W+N+  D  +V+L GDSAGGNI+H + +R   E L    I G+ LIHP+F+   
Sbjct: 137 GTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSLISGIILIHPYFWSKT 196

Query: 63  KIDR-------RRKGVGSKGYICGEESVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAE 122
            ID        + KGV     +    S   + +D P +N     D   L   +V V VA 
Sbjct: 197 PIDEFEVRDVGKTKGVEGSWRVASPNSK--QGVDDPWLN-VVGSDPSGLGCGRVLVMVAG 256

Query: 123 KDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
            D    +G  YAE L+ SGW+G VEV+ET +EGHVFHL NP SD+A
Sbjct: 257 DDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNA 299

[Close]

BLAST of CmaCh20G008960 vs. Swiss-Prot
Match: CXE12_ARATH (Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 3.6e-26
Identity = 72/172 (41.86%), Postives = 93/172 (54.07%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATE----GLAGVKIKGLQLIHPHF 62
           G+G ++WLN+ AD +RV+L GDSAG NI H MA+R A E    GL    I G+ L+HP+F
Sbjct: 140 GSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPGLNDTGISGIILLHPYF 199

Query: 63  FGG--------KIDRRRKGVGSKGYICGEESVDIKELDHPIVN--PEYDPDLGWLPAEKV 122
           +          K +  R  + +   +    S D    D P++N       DL  L   KV
Sbjct: 200 WSKTPIDEKDTKDETLRMKIEAFWMMASPNSKD--GTDDPLLNVVQSESVDLSGLGCGKV 259

Query: 123 AVYVAEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
            V VAEKD L  +G  YA  L  SGW G VEVVE++ E HVFHL  P  D+A
Sbjct: 260 LVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLKPECDNA 309

[Close]

BLAST of CmaCh20G008960 vs. Swiss-Prot
Match: CXE4_ARATH (Probable carboxylesterase 4 OS=Arabidopsis thaliana GN=CXE4 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 6.8e-25
Identity = 70/168 (41.67%), Postives = 93/168 (55.36%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG-G 62
           G+G ++W+N+ AD  RV+L GDSAGGNI+H MA+R   E L   +IKG  ++HP  +G  
Sbjct: 197 GSGEEDWINKYADFERVFLAGDSAGGNISHHMAMRAGKEKLK-PRIKGTVIVHPAIWGKD 256

Query: 63  KIDRR-------RKGVGSK-GYICGEESVDIKELDHPIVN-PEYDPDLGWLPAEKVAVYV 122
            +D         R GV      I    SVD    D P  N      +   +  +KV V V
Sbjct: 257 PVDEHDVQDREIRDGVAEVWEKIVSPNSVD--GADDPWFNVVGSGSNFSGMGCDKVLVEV 316

Query: 123 AEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           A KD    +GL YA  L+ SGW G VEV+E +DE H FHL NP+S++A
Sbjct: 317 AGKDVFWRQGLAYAAKLKKSGWKGEVEVIEEEDEEHCFHLLNPSSENA 361

[Close]

BLAST of CmaCh20G008960 vs. TrEMBL
Match: A0A0A0LKY5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234510 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 2.9e-59
Identity = 115/163 (70.55%), Postives = 125/163 (76.69%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGK 62
           G GPDEWLNEIADLN+VYL GDSAGGN+AHRMALR  TEGL GVKIKGLQLIHPHF+GG+
Sbjct: 139 GKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGVKIKGLQLIHPHFWGGE 198

Query: 63  IDRRRKGVGSKGYICGEE-----SVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAEKDN 122
           +         K     E      S DIK LD PIVNPE+DPDLG LPAE+V +YVAEKDN
Sbjct: 199 LLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPDLGRLPAERVGIYVAEKDN 258

Query: 123 LKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           LKERG  YAECL+ SGW G VEVVET+ EGHVFHLFNPT D A
Sbjct: 259 LKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDMA 301

[Close]

BLAST of CmaCh20G008960 vs. TrEMBL
Match: A0A0A0LIH8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234520 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 7.5e-47
Identity = 94/169 (55.62%), Postives = 121/169 (71.60%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGK 62
           G GP+EWLN+IAD NRVY  GDSAG N+A++MA+RV  EG+AG+ +KGL L+HP+F+G K
Sbjct: 139 GTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVAGLNLKGLMLVHPYFWGEK 198

Query: 63  IDRRRKGVGSKGYICGEESVDIKEL-----------DHPIVNPEYDPDLGWLPAEKVAVY 122
           +      +G +  +  EE   I++L           D PIVNPE++P+LG + AE+VAVY
Sbjct: 199 L------IGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPNLGKVTAERVAVY 258

Query: 123 VAEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           VAEKD LK+RG  Y+ECL+ SGW G VEV ET  +GHVFHLFNPTSD A
Sbjct: 259 VAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNPTSDDA 301

[Close]

BLAST of CmaCh20G008960 vs. TrEMBL
Match: M5W2V0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007279mg PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 8.3e-38
Identity = 81/166 (48.80%), Postives = 106/166 (63.86%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGK 62
           G GP+EWLN  AD  RV+  GDSAG NIAH MALR+  EGL GVK+KG+ L+HP+F+G +
Sbjct: 199 GQGPEEWLNSYADFERVFFAGDSAGANIAHHMALRLGHEGLVGVKLKGIALVHPYFWGSE 258

Query: 63  --------IDRRRKGVGSKGYICGEESVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAE 122
                   ++ R +  G   + C   S      D P++NP  DP L  L A++V V VAE
Sbjct: 259 PIEGETHVVENRARAEGIWRFACPSSS----GADDPLINPGKDPKLAKLGADRVLVCVAE 318

Query: 123 KDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           +D L++RG  Y+E L+ S W G VEVVET +E HVFHL NPT ++A
Sbjct: 319 QDVLRQRGWYYSELLKKSEWGGAVEVVETKEEDHVFHLNNPTGENA 360

[Close]

BLAST of CmaCh20G008960 vs. TrEMBL
Match: U5G0L2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s10680g PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.4e-37
Identity = 79/168 (47.02%), Postives = 111/168 (66.07%), Query Frame = 1


Query: 1   MGGAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG 60
           + G GP+EWLN+ ADL++V+L GDSAGGNIAH +A+R   E + GV + G+ LI+P+F+G
Sbjct: 144 VNGDGPEEWLNKHADLSKVFLAGDSAGGNIAHHVAMRFGQEKIIGVNVAGIVLINPYFWG 203

Query: 61  --------GKIDRRRKGVGSKGYICGEESVDIKELDHPIVNPEYDPDLGWLPAEKVAVYV 120
                    +++R  KG+ +  ++   ++      D P++NP YDP+L  L   KV V V
Sbjct: 204 EERIGNEVNELERELKGMSATWHLACPKT---SGCDDPLINPTYDPNLSSLGCSKVFVSV 263

Query: 121 AEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           AEKD L++RGL Y E L+ SGW GV+E +E   EGHVFHLF P SD+A
Sbjct: 264 AEKDLLRDRGLLYCETLKKSGWVGVIETMEVKGEGHVFHLFKPASDNA 308

[Close]

BLAST of CmaCh20G008960 vs. TrEMBL
Match: B9HRY8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s10700g PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.9e-37
Identity = 79/168 (47.02%), Postives = 111/168 (66.07%), Query Frame = 1


Query: 1   MGGAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG 60
           + G GP+EWLN+ ADL++V+L GDSAGGNIAH +A+R   E + GV + G+ LI+P+F+G
Sbjct: 144 VNGDGPEEWLNKHADLSKVFLAGDSAGGNIAHHVAMRFGQEKIIGVNVAGIVLINPYFWG 203

Query: 61  --------GKIDRRRKGVGSKGYICGEESVDIKELDHPIVNPEYDPDLGWLPAEKVAVYV 120
                    +++R  KG+ +  ++   ++      D P++NP YDP+L  L   KV V V
Sbjct: 204 EEPIGNEVNELERVLKGISATWHLACPKT---SGCDDPLINPTYDPNLSSLGCSKVFVSV 263

Query: 121 AEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           AEKD L++RGL Y E L+ SGW GV+E +E   EGHVFHLF P SD+A
Sbjct: 264 AEKDLLRDRGLLYCETLKKSGWVGVIETMEVKGEGHVFHLFKPASDNA 308

[Close]

BLAST of CmaCh20G008960 vs. TAIR10
Match: AT1G47480.1 (AT1G47480.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 125.9 bits (315), Expect = 2.2e-29
Identity = 72/164 (43.90%), Postives = 98/164 (59.76%), Query Frame = 1


Query: 7   DEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGKI--- 66
           + W+N+ ADL+ ++L GDSAG NI+H +A R A +    +KIKG+ +IHP+F+G +    
Sbjct: 140 EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQTLKIKGIGMIHPYFWGTQPIGA 199

Query: 67  ---DRRRKGV--GSKGYICGEESVDIKELDHPIVNPEYD--PDLGWLPAEKVAVYVAEKD 126
              D  RK +  G   ++C  E    K  D P +NP  D  PDLG L  E+V + VAEKD
Sbjct: 200 EIKDEARKQMVDGWWEFVCPSE----KGSDDPWINPFADGSPDLGGLGCERVMITVAEKD 259

Query: 127 NLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
            L ERG  Y E L  S W G VE++ET ++ HVFH+F P  D A
Sbjct: 260 ILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEA 298

[Close]

BLAST of CmaCh20G008960 vs. TAIR10
Match: AT1G49660.1 (AT1G49660.1 carboxyesterase 5)

HSP 1 Score: 122.1 bits (305), Expect = 3.1e-28
Identity = 69/166 (41.57%), Postives = 92/166 (55.42%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG-- 62
           G+GP +W+N+ AD  +V+LGGDSAGGNI+H MA++   E    +KIKG+ ++HP F+G  
Sbjct: 141 GSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKLDLKIKGIAVVHPAFWGTD 200

Query: 63  --GKIDRRRKGVGSKGYICGEESV---DIKELDHPIVNPE-YDPDLGWLPAEKVAVYVAE 122
              + D + K   S      E+      +   D P+ N      D   L  +KV V VA 
Sbjct: 201 PVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSGSDFSGLGCDKVLVAVAG 260

Query: 123 KDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           KD    +GL YA  L    W+G VEVVE + E HVFHL NP SD A
Sbjct: 261 KDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKSDKA 306

[Close]

BLAST of CmaCh20G008960 vs. TAIR10
Match: AT2G03550.1 (AT2G03550.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 119.8 bits (299), Expect = 1.6e-27
Identity = 69/166 (41.57%), Postives = 92/166 (55.42%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG-G 62
           G GP+ W+N+  D  +V+L GDSAGGNI+H + +R   E L    I G+ LIHP+F+   
Sbjct: 137 GTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSLISGIILIHPYFWSKT 196

Query: 63  KIDR-------RRKGVGSKGYICGEESVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAE 122
            ID        + KGV     +    S   + +D P +N     D   L   +V V VA 
Sbjct: 197 PIDEFEVRDVGKTKGVEGSWRVASPNSK--QGVDDPWLN-VVGSDPSGLGCGRVLVMVAG 256

Query: 123 KDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
            D    +G  YAE L+ SGW+G VEV+ET +EGHVFHL NP SD+A
Sbjct: 257 DDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNA 299

[Close]

BLAST of CmaCh20G008960 vs. TAIR10
Match: AT3G48690.1 (AT3G48690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 119.4 bits (298), Expect = 2.0e-27
Identity = 72/172 (41.86%), Postives = 93/172 (54.07%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATE----GLAGVKIKGLQLIHPHF 62
           G+G ++WLN+ AD +RV+L GDSAG NI H MA+R A E    GL    I G+ L+HP+F
Sbjct: 140 GSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPGLNDTGISGIILLHPYF 199

Query: 63  FGG--------KIDRRRKGVGSKGYICGEESVDIKELDHPIVN--PEYDPDLGWLPAEKV 122
           +          K +  R  + +   +    S D    D P++N       DL  L   KV
Sbjct: 200 WSKTPIDEKDTKDETLRMKIEAFWMMASPNSKD--GTDDPLLNVVQSESVDLSGLGCGKV 259

Query: 123 AVYVAEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
            V VAEKD L  +G  YA  L  SGW G VEVVE++ E HVFHL  P  D+A
Sbjct: 260 LVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLKPECDNA 309

[Close]

BLAST of CmaCh20G008960 vs. TAIR10
Match: AT1G49650.1 (AT1G49650.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 115.2 bits (287), Expect = 3.8e-26
Identity = 70/168 (41.67%), Postives = 93/168 (55.36%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFG-G 62
           G+G ++W+N+ AD  RV+L GDSAGGNI+H MA+R   E L   +IKG  ++HP  +G  
Sbjct: 197 GSGEEDWINKYADFERVFLAGDSAGGNISHHMAMRAGKEKLK-PRIKGTVIVHPAIWGKD 256

Query: 63  KIDRR-------RKGVGSK-GYICGEESVDIKELDHPIVN-PEYDPDLGWLPAEKVAVYV 122
            +D         R GV      I    SVD    D P  N      +   +  +KV V V
Sbjct: 257 PVDEHDVQDREIRDGVAEVWEKIVSPNSVD--GADDPWFNVVGSGSNFSGMGCDKVLVEV 316

Query: 123 AEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           A KD    +GL YA  L+ SGW G VEV+E +DE H FHL NP+S++A
Sbjct: 317 AGKDVFWRQGLAYAAKLKKSGWKGEVEVIEEEDEEHCFHLLNPSSENA 361

[Close]

BLAST of CmaCh20G008960 vs. NCBI nr
Match: gi|449455336|ref|XP_004145409.1| (PREDICTED: probable carboxylesterase 12 [Cucumis sativus])

HSP 1 Score: 236.1 bits (601), Expect = 4.2e-59
Identity = 115/163 (70.55%), Postives = 125/163 (76.69%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGK 62
           G GPDEWLNEIADLN+VYL GDSAGGN+AHRMALR  TEGL GVKIKGLQLIHPHF+GG+
Sbjct: 139 GKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGVKIKGLQLIHPHFWGGE 198

Query: 63  IDRRRKGVGSKGYICGEE-----SVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAEKDN 122
           +         K     E      S DIK LD PIVNPE+DPDLG LPAE+V +YVAEKDN
Sbjct: 199 LLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPDLGRLPAERVGIYVAEKDN 258

Query: 123 LKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           LKERG  YAECL+ SGW G VEVVET+ EGHVFHLFNPT D A
Sbjct: 259 LKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDMA 301

[Close]

BLAST of CmaCh20G008960 vs. NCBI nr
Match: gi|659118607|ref|XP_008459207.1| (PREDICTED: probable carboxylesterase 12 [Cucumis melo])

HSP 1 Score: 232.3 bits (591), Expect = 6.1e-58
Identity = 114/163 (69.94%), Postives = 123/163 (75.46%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGK 62
           G G DEWLNEIADLN+VYL GDSAGGN+AHRMALR  TEGL GVKIKGLQLIHPHF+GGK
Sbjct: 139 GKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGVKIKGLQLIHPHFWGGK 198

Query: 63  IDRRRKGVGSKGYICGEE-----SVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAEKDN 122
           +               E      S DIK LD PIVNPE+DPDLG +PAE+V VYVAEKDN
Sbjct: 199 LIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPEHDPDLGRMPAERVGVYVAEKDN 258

Query: 123 LKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           LKERG  YAECL+ SGW G VEVVET+ EGHVFHLFNPT D A
Sbjct: 259 LKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDIA 301

[Close]

BLAST of CmaCh20G008960 vs. NCBI nr
Match: gi|659118611|ref|XP_008459209.1| (PREDICTED: probable carboxylesterase 7 [Cucumis melo])

HSP 1 Score: 200.7 bits (509), Expect = 2.0e-48
Identity = 99/166 (59.64%), Postives = 122/166 (73.49%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGK 62
           G GP+EWLNEIADLNRVY  GDSAG NIA++MA+RV  EG+AG+ +KGL LIHP+F+G K
Sbjct: 210 GTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIEGVAGLNLKGLMLIHPYFWGEK 269

Query: 63  I--DRRRKGVGSKGYICGEESV------DIKELDHPIVNPEYDPDLGWLPAEKVAVYVAE 122
           +  D  +     + +I   E +       I  LD P+VNPE++P LG L AE+VAVYVAE
Sbjct: 270 LIGDEEKLKPEDRWFI---EKLWYLACPTISGLDDPVVNPEFEPKLGGLAAERVAVYVAE 329

Query: 123 KDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           KD+LK+RG  Y+ECL+ SGW G VEVVET  +GHVFHLFNPTSD A
Sbjct: 330 KDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPTSDDA 372

[Close]

BLAST of CmaCh20G008960 vs. NCBI nr
Match: gi|449474681|ref|XP_004154254.1| (PREDICTED: probable carboxylesterase 13 [Cucumis sativus])

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-46
Identity = 94/169 (55.62%), Postives = 121/169 (71.60%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGGK 62
           G GP+EWLN+IAD NRVY  GDSAG N+A++MA+RV  EG+AG+ +KGL L+HP+F+G K
Sbjct: 139 GTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVAGLNLKGLMLVHPYFWGEK 198

Query: 63  IDRRRKGVGSKGYICGEESVDIKEL-----------DHPIVNPEYDPDLGWLPAEKVAVY 122
           +      +G +  +  EE   I++L           D PIVNPE++P+LG + AE+VAVY
Sbjct: 199 L------IGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPNLGKVTAERVAVY 258

Query: 123 VAEKDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           VAEKD LK+RG  Y+ECL+ SGW G VEV ET  +GHVFHLFNPTSD A
Sbjct: 259 VAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNPTSDDA 301

[Close]

BLAST of CmaCh20G008960 vs. NCBI nr
Match: gi|1009115549|ref|XP_015874288.1| (PREDICTED: probable carboxylesterase 12 [Ziziphus jujuba])

HSP 1 Score: 166.8 bits (421), Expect = 3.1e-38
Identity = 81/166 (48.80%), Postives = 107/166 (64.46%), Query Frame = 1


Query: 3   GAGPDEWLNEIADLNRVYLGGDSAGGNIAHRMALRVATEGLAGVKIKGLQLIHPHFFGG- 62
           G GP++WLN   D  RV+ GGDSAG NIAH MALRV ++GL G K+ G+ LIHP+F+G  
Sbjct: 246 GKGPEDWLNSYVDYQRVFFGGDSAGANIAHHMALRVGSDGLGGPKLVGIALIHPYFWGST 305

Query: 63  -------KIDRRRKGVGSKGYICGEESVDIKELDHPIVNPEYDPDLGWLPAEKVAVYVAE 122
                  ++++RR   G   + C   S     LD P++NP+ DP+LG L  E+V V VAE
Sbjct: 306 PIGSESTQVEKRRMAGGIWMFACPTTS----GLDDPLINPDKDPNLGKLGGERVLVCVAE 365

Query: 123 KDNLKERGLQYAECLRNSGWDGVVEVVETDDEGHVFHLFNPTSDSA 161
           KD LK+RG  Y E L+ +GW G VEV E  DE HV+H+F PT ++A
Sbjct: 366 KDVLKDRGWYYGELLKRNGWGGTVEVDEAKDEEHVYHMFKPTCENA 407

The following BLAST results are available for this feature:

BLAST of CmaCh20G008960 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CXE2_ARATH	3.8e-28	43.90	Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1	[more]
CXE5_ARATH	5.6e-27	41.57	Probable carboxylesterase 5 OS=Arabidopsis thaliana GN=CXE5 PE=2 SV=1	[more]
CXE7_ARATH	2.8e-26	41.57	Probable carboxylesterase 7 OS=Arabidopsis thaliana GN=CXE7 PE=2 SV=1	[more]
CXE12_ARATH	3.6e-26	41.86	Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1	[more]
CXE4_ARATH	6.8e-25	41.67	Probable carboxylesterase 4 OS=Arabidopsis thaliana GN=CXE4 PE=2 SV=1	[more]

BLAST of CmaCh20G008960 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKY5_CUCSA	2.9e-59	70.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234510 PE=4 SV=1	[more]
A0A0A0LIH8_CUCSA	7.5e-47	55.62	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234520 PE=4 SV=1	[more]
M5W2V0_PRUPE	8.3e-38	48.80	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007279mg PE=4 SV=1	[more]
U5G0L2_POPTR	1.4e-37	47.02	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s10680g PE=4 SV=1	[more]
B9HRY8_POPTR	1.9e-37	47.02	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0009s10700g PE=4 SV=1	[more]

BLAST of CmaCh20G008960 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G47480.1	2.2e-29	43.90	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	3.1e-28	41.57	carboxyesterase 5	[more]
AT2G03550.1	1.6e-27	41.57	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48690.1	2.0e-27	41.86	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49650.1	3.8e-26	41.67	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of CmaCh20G008960 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455336\|ref\|XP_004145409.1\|	4.2e-59	70.55	PREDICTED: probable carboxylesterase 12 [Cucumis sativus]	[more]
gi\|659118607\|ref\|XP_008459207.1\|	6.1e-58	69.94	PREDICTED: probable carboxylesterase 12 [Cucumis melo]	[more]
gi\|659118611\|ref\|XP_008459209.1\|	2.0e-48	59.64	PREDICTED: probable carboxylesterase 7 [Cucumis melo]	[more]
gi\|449474681\|ref\|XP_004154254.1\|	1.1e-46	55.62	PREDICTED: probable carboxylesterase 13 [Cucumis sativus]	[more]
gi\|1009115549\|ref\|XP_015874288.1\|	3.1e-38	48.80	PREDICTED: probable carboxylesterase 12 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G008960.1	CmaCh20G008960.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 9..151 score: 3.0
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 7..160 score: 2.0
None	No IPR available	PANTHER	PTHR23024:SF157	CARBOXYLESTERASE 12-RELATED	coord: 7..160 score: 2.0

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh20G008960	CmaCh02G004450	Cucurbita maxima (Rimu)	cmacmaB469

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh20G008960	Cucumber (Chinese Long) v2	cmacuB542
CmaCh20G008960	Melon (DHL92) v3.5.1	cmameB495
CmaCh20G008960	Watermelon (Charleston Gray)	cmawcgB474
CmaCh20G008960	Watermelon (97103) v1	cmawmB509
CmaCh20G008960	Cucurbita pepo (Zucchini)	cmacpeB565
CmaCh20G008960	Cucurbita pepo (Zucchini)	cmacpeB585
CmaCh20G008960	Bottle gourd (USVL1VR-Ls)	cmalsiB507
CmaCh20G008960	Cucumber (Gy14) v2	cgybcmaB218
CmaCh20G008960	Melon (DHL92) v3.6.1	cmamedB575
CmaCh20G008960	Silver-seed gourd	carcmaB1104
CmaCh20G008960	Silver-seed gourd	carcmaB1459
CmaCh20G008960	Cucumber (Chinese Long) v3	cmacucB0651
CmaCh20G008960	Watermelon (97103) v2	cmawmbB598
CmaCh20G008960	Wax gourd	cmawgoB0699
CmaCh20G008960	Cucumber (Gy14) v1	cgycmaB0160
CmaCh20G008960	Cucurbita moschata (Rifu)	cmacmoB551
CmaCh20G008960	Cucurbita moschata (Rifu)	cmacmoB554
CmaCh20G008960	Wild cucumber (PI 183967)	cmacpiB547